##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939066_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 379863 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.64413749167463 34.0 31.0 34.0 31.0 34.0 2 32.74806443375638 34.0 33.0 34.0 31.0 34.0 3 32.75404816999813 34.0 31.0 34.0 31.0 34.0 4 36.17651100528349 37.0 37.0 37.0 35.0 37.0 5 36.21484851117376 37.0 37.0 37.0 35.0 37.0 6 36.3365634452421 37.0 37.0 37.0 35.0 37.0 7 36.30532060242772 37.0 37.0 37.0 35.0 37.0 8 36.31759608069225 37.0 37.0 37.0 35.0 37.0 9 38.109994919220874 39.0 39.0 39.0 37.0 39.0 10-11 38.06069030150344 39.0 39.0 39.0 37.0 39.0 12-13 38.00249432032075 39.0 39.0 39.0 37.0 39.0 14-15 39.355051689688125 41.0 39.5 41.0 37.0 41.0 16-17 39.29859449327784 41.0 39.0 41.0 37.0 41.0 18-19 39.266783024406166 41.0 39.0 41.0 36.5 41.0 20-21 39.26531933881426 41.0 39.0 41.0 36.0 41.0 22-23 39.238308284829 41.0 39.0 41.0 35.5 41.0 24-25 39.17990696645896 41.0 39.0 41.0 35.0 41.0 26-27 39.035568086389034 41.0 39.0 41.0 35.0 41.0 28-29 38.86024566751698 41.0 39.0 41.0 35.0 41.0 30-31 38.51757080842303 40.0 37.5 41.0 35.0 41.0 32-33 38.13768253291318 40.0 36.5 41.0 35.0 41.0 34-35 37.814164580388194 40.0 35.0 41.0 35.0 41.0 36-37 37.55518831789355 40.0 35.0 41.0 35.0 41.0 38-39 37.3466947294156 40.0 35.0 41.0 35.0 41.0 40-41 37.12282191211042 40.0 35.0 41.0 34.5 41.0 42-43 36.831518205247676 40.0 35.0 41.0 33.5 41.0 44-45 36.42444512890174 40.0 35.0 41.0 33.0 41.0 46-47 35.91337008342481 40.0 35.0 41.0 31.0 41.0 48-49 35.58858588491114 40.0 35.0 41.0 28.5 41.0 50-51 34.448287935387235 38.5 34.0 40.0 24.5 40.5 52-53 34.6003243274549 38.5 35.0 40.0 24.0 40.5 54-55 35.06010588027789 39.5 35.0 41.0 23.5 41.0 56-57 34.95268425721905 40.0 35.0 41.0 21.5 41.0 58-59 34.75997135809489 39.0 35.0 41.0 18.5 41.0 60-61 34.50353416889773 39.0 35.0 41.0 7.0 41.0 62-63 34.151865277744875 39.0 35.0 41.0 2.0 41.0 64-65 33.81631930459139 38.0 35.0 40.5 2.0 41.0 66-67 33.467215812016434 37.5 35.0 40.0 2.0 41.0 68-69 33.07038721854985 37.0 35.0 40.0 2.0 41.0 70-71 32.64713857364366 36.5 34.5 39.0 2.0 41.0 72-73 32.18432829730719 36.0 34.0 39.0 2.0 41.0 74-75 31.726571158549266 35.0 34.0 38.5 2.0 40.0 76-77 31.27959290586343 35.0 33.0 37.0 2.0 39.5 78-79 30.822293826984993 35.0 33.0 37.0 2.0 39.0 80-81 30.351179240936865 35.0 33.0 36.5 2.0 39.0 82-83 29.960030326723057 35.0 32.5 36.0 2.0 37.5 84-85 29.510422178522255 35.0 32.0 35.5 2.0 37.0 86-87 29.088192321968712 35.0 31.0 35.0 2.0 37.0 88-89 28.652585274164636 35.0 31.0 35.0 2.0 36.0 90-91 28.231586124471193 35.0 30.0 35.0 2.0 36.0 92-93 27.823997072628817 34.0 29.0 35.0 2.0 36.0 94-95 27.38804911244317 34.0 29.0 35.0 2.0 35.0 96-97 26.903158507145996 34.0 26.5 35.0 2.0 35.0 98-99 26.406530775569088 34.0 25.0 35.0 2.0 35.0 100-101 23.950576918520625 31.5 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.09823254054906272 1101 2 0.0826063600239948 1101 3 0.03934387866576827 1101 4 0.09201679510449168 1101 5 -0.008435893188007526 1101 6 0.0405093298572794 1101 7 -0.10681461538025161 1101 8 -0.09823640432448144 1101 9 -0.012803076596270557 1101 10-11 0.1788283917773228 1101 12-13 0.21684029375624903 1101 14-15 0.402788842442277 1101 16-17 0.41032161852550786 1101 18-19 0.4116541154312543 1101 20-21 0.24794338224717194 1101 22-23 0.34394560207176994 1101 24-25 0.2656707154098612 1101 26-27 0.23855687710239692 1101 28-29 0.4000058399090278 1101 30-31 0.4000763382198542 1101 32-33 0.437755254444987 1101 34-35 0.6239678867505631 1101 36-37 0.49703073993620706 1101 38-39 0.31636538451432017 1101 40-41 0.17785242059972717 1101 42-43 0.21871024717511034 1101 44-45 0.26952817183128985 1101 46-47 0.5663907337364051 1101 48-49 0.454817482735244 1101 50-51 0.3416546848268851 1101 52-53 0.41862656835151313 1101 54-55 0.48760409088671963 1101 56-57 0.49716939124303394 1101 58-59 0.3158996024676668 1101 60-61 0.24050091043186228 1101 62-63 0.35148134800176933 1101 64-65 0.4596830738022817 1101 66-67 0.42949463686863254 1101 68-69 0.5107970814760634 1101 70-71 0.3484287341088077 1101 72-73 0.23872046475447206 1101 74-75 0.3825482547811596 1101 76-77 0.5282433500761456 1101 78-79 0.13914389754119583 1101 80-81 -0.023117117561120892 1101 82-83 0.06739746511366818 1101 84-85 0.1509714532857096 1101 86-87 0.20763948111480346 1101 88-89 0.4852837894237858 1101 90-91 0.34975547120436445 1101 92-93 0.6985189807035965 1101 94-95 0.4625811773869124 1101 96-97 0.40182459526841896 1101 98-99 0.16096719931778836 1101 100-101 0.40317643080243215 1102 1 0.08326477041904923 1102 2 0.08421785352339128 1102 3 0.06119463797965352 1102 4 0.023924366392591878 1102 5 -0.05992174482135226 1102 6 0.03370828102082868 1102 7 0.08121116851573618 1102 8 -0.010833367068940447 1102 9 0.1402614923974994 1102 10-11 0.043831669391217076 1102 12-13 0.01761599571189265 1102 14-15 0.09838045175069965 1102 16-17 0.12378988029760052 1102 18-19 0.026466198901154314 1102 20-21 0.07095223180468935 1102 22-23 0.20438753367889007 1102 24-25 0.2630349675676129 1102 26-27 0.14418891031554892 1102 28-29 0.040246744530456624 1102 30-31 0.4102779114745303 1102 32-33 0.4500872767327664 1102 34-35 0.0870127900609532 1102 36-37 0.1367663457485051 1102 38-39 0.2051177061575018 1102 40-41 0.21225370979453118 1102 42-43 0.041882977427484036 1102 44-45 -0.0038931108082849164 1102 46-47 0.2586910724231828 1102 48-49 0.3828738576942712 1102 50-51 0.5282738260920468 1102 52-53 0.7296857839092539 1102 54-55 0.606444908105118 1102 56-57 0.692364955538892 1102 58-59 0.5840493894227592 1102 60-61 0.5129389089023064 1102 62-63 0.7085364119152473 1102 64-65 0.7798513246490018 1102 66-67 0.6190499739165958 1102 68-69 0.7423195331312584 1102 70-71 0.6396037586909102 1102 72-73 0.9672247802794303 1102 74-75 0.9664688272710471 1102 76-77 0.910850145662291 1102 78-79 0.6445246470222372 1102 80-81 0.43798853086016365 1102 82-83 0.38964153809520496 1102 84-85 0.443935508786641 1102 86-87 0.42876916537132814 1102 88-89 0.5597402517858541 1102 90-91 0.4789890558214296 1102 92-93 0.4353129280432668 1102 94-95 0.5573315324617489 1102 96-97 0.7914782607332924 1102 98-99 0.8326322271775055 1102 100-101 0.291478080534759 1103 1 0.05486970869065999 1103 2 0.16838633573109263 1103 3 0.28991649709367096 1103 4 0.09712698077167659 1103 5 0.1967957569941916 1103 6 0.20407865139119963 1103 7 0.15731864854478772 1103 8 0.1922893635047771 1103 9 0.1368845860794181 1103 10-11 0.22662034041518098 1103 12-13 0.2691047393575019 1103 14-15 0.4005591009881684 1103 16-17 0.289113046601166 1103 18-19 0.3273013477755171 1103 20-21 0.5332071337654796 1103 22-23 0.5172234087696737 1103 24-25 0.45985777076297296 1103 26-27 0.4818069204825761 1103 28-29 0.4874689667526866 1103 30-31 0.4679576500366167 1103 32-33 0.5625600436172888 1103 34-35 0.44474699485397196 1103 36-37 0.4836436151747918 1103 38-39 0.6894132762373886 1103 40-41 0.8695884955606843 1103 42-43 1.0435714305865318 1103 44-45 1.1014082690029028 1103 46-47 0.9725073396708197 1103 48-49 1.081348803228046 1103 50-51 0.9896028021269032 1103 52-53 0.7409239828925536 1103 54-55 0.7333875370085323 1103 56-57 0.8623177514720837 1103 58-59 1.0851387140414985 1103 60-61 1.1313303395486471 1103 62-63 0.8003664772747214 1103 64-65 0.7548331692386867 1103 66-67 0.7816679840836187 1103 68-69 0.7520689884689489 1103 70-71 0.7340482031353517 1103 72-73 0.9672429356897467 1103 74-75 0.822314869391608 1103 76-77 0.9261914673761602 1103 78-79 0.8822334342408311 1103 80-81 1.0792557784999595 1103 82-83 1.1353568612615064 1103 84-85 1.1126537368185971 1103 86-87 0.9675128109777198 1103 88-89 1.0927104769129414 1103 90-91 1.0543884748482988 1103 92-93 1.2004363848333917 1103 94-95 0.89440488031941 1103 96-97 1.0220701271094725 1103 98-99 1.134865342645913 1103 100-101 1.0658607965841433 1104 1 0.023441137262082634 1104 2 0.2535123861512574 1104 3 0.10831985843820746 1104 4 -0.09052007805185269 1104 5 -0.052531974098251055 1104 6 -0.07449277718022529 1104 7 -0.1043620237241214 1104 8 -0.10804677094900939 1104 9 -0.013283481147475129 1104 10-11 -0.07333764277809252 1104 12-13 0.008180369609604554 1104 14-15 0.0075338909041420266 1104 16-17 -0.06630712146606044 1104 18-19 -0.051144030375738225 1104 20-21 -0.005238244385786572 1104 22-23 -0.12160012064209269 1104 24-25 -0.08644475024543397 1104 26-27 -0.01785694506364166 1104 28-29 0.0517546810383962 1104 30-31 -0.16742050122388008 1104 32-33 -0.23290214125665898 1104 34-35 -0.2239925009443482 1104 36-37 -0.020305964657133302 1104 38-39 -0.05735142964496731 1104 40-41 -0.3918820926746065 1104 42-43 -0.14134453580002315 1104 44-45 -0.14552450410634066 1104 46-47 -0.1653918199930473 1104 48-49 0.06260930742971738 1104 50-51 0.1854431382613555 1104 52-53 0.17974751230431707 1104 54-55 0.1868329151597905 1104 56-57 0.21904044054770822 1104 58-59 0.05576896614233817 1104 60-61 0.028221095851158395 1104 62-63 -0.009003270624440063 1104 64-65 0.18928695075129554 1104 66-67 0.09271840425169131 1104 68-69 0.25610260191432843 1104 70-71 0.33047677456392677 1104 72-73 0.4006462970342852 1104 74-75 0.5174829366184959 1104 76-77 0.347746089224902 1104 78-79 0.27815780398873 1104 80-81 0.3408104003486976 1104 82-83 0.21930644109344044 1104 84-85 0.4443764258942231 1104 86-87 0.1531430630785593 1104 88-89 0.008928964307902021 1104 90-91 0.11926242442812907 1104 92-93 -0.3595636151666106 1104 94-95 -0.5859312541343691 1104 96-97 -0.3361231502014519 1104 98-99 -0.5908069262616493 1104 100-101 -0.7141392034158578 1105 1 -0.0610847072637597 1105 2 -0.0484981958593238 1105 3 0.017509927628282185 1105 4 0.05740014986837849 1105 5 0.015339413773141075 1105 6 -0.04392704661449898 1105 7 -0.11062168998378752 1105 8 0.07938505648282046 1105 9 -0.06486671273070499 1105 10-11 -0.06906006850051938 1105 12-13 -0.05170974028050779 1105 14-15 -0.10033749696724925 1105 16-17 -0.1268036619626045 1105 18-19 -0.11728973652144248 1105 20-21 -0.16124150038903196 1105 22-23 -0.16467704371901704 1105 24-25 0.034283780483100657 1105 26-27 0.009098691891992416 1105 28-29 -0.1645253352416134 1105 30-31 -0.2325283563317342 1105 32-33 -0.2311805295350453 1105 34-35 -0.12452567147751381 1105 36-37 0.006661882310716294 1105 38-39 -0.12655370884724704 1105 40-41 -0.2102296710221836 1105 42-43 -0.21167827613376744 1105 44-45 -0.6960820946639288 1105 46-47 -0.5462709408721693 1105 48-49 -0.5202600454396347 1105 50-51 -0.5981506753324553 1105 52-53 -0.4062353936785925 1105 54-55 -0.24944303111615795 1105 56-57 -0.1372884157811498 1105 58-59 -0.2767055963322207 1105 60-61 -0.3490031613730977 1105 62-63 -0.40934108096224975 1105 64-65 -0.5240423125779756 1105 66-67 -0.34438782285453584 1105 68-69 -0.40547248966076666 1105 70-71 -0.4668777227905707 1105 72-73 -0.512357365969379 1105 74-75 -0.5426717235361629 1105 76-77 -0.7377728362940239 1105 78-79 -0.791610205779282 1105 80-81 -0.7790105194722194 1105 82-83 -0.9531884714014751 1105 84-85 -1.2317530002352015 1105 86-87 -1.1548015848660889 1105 88-89 -1.2503262249472904 1105 90-91 -0.9040831189174128 1105 92-93 -1.0512730168760136 1105 94-95 -1.2430008512039663 1105 96-97 -1.171959536037832 1105 98-99 -1.645288000742724 1105 100-101 -1.9322873515640033 1106 1 0.2045964846469417 1106 2 0.2559948217484518 1106 3 0.21764070152954673 1106 4 0.21745806402465462 1106 5 0.14464210864960592 1106 6 0.16773985357699672 1106 7 0.08100878033521042 1106 8 0.20784706199400205 1106 9 0.1542262768540894 1106 10-11 0.255959781109496 1106 12-13 0.2162599949023445 1106 14-15 0.300414452965029 1106 16-17 0.14203011506788954 1106 18-19 0.33827048953058636 1106 20-21 0.3332473137719063 1106 22-23 0.3553944148770327 1106 24-25 0.26331325131585004 1106 26-27 0.361604413250177 1106 28-29 0.20304273724448763 1106 30-31 0.25567864007197727 1106 32-33 0.20881526701813158 1106 34-35 0.32820890420788373 1106 36-37 0.4221842773413087 1106 38-39 0.4381676960381 1106 40-41 0.4555496013144662 1106 42-43 0.42424484749427194 1106 44-45 0.28089235772035437 1106 46-47 0.42882363668142176 1106 48-49 0.3101659038387794 1106 50-51 0.342101998526779 1106 52-53 0.5967420478234473 1106 54-55 0.6058883406086579 1106 56-57 0.46728400026668027 1106 58-59 0.5388915266419474 1106 60-61 0.4408128210658404 1106 62-63 0.29619579260740636 1106 64-65 0.3079832817427075 1106 66-67 0.2599627446107817 1106 68-69 0.3069066612429694 1106 70-71 0.3183378206216929 1106 72-73 0.25739883411468867 1106 74-75 0.19964530976447747 1106 76-77 0.26912782224598075 1106 78-79 0.3783841896249527 1106 80-81 0.242683936648465 1106 82-83 0.10801838488735171 1106 84-85 -0.004101325862215788 1106 86-87 0.13014540499893457 1106 88-89 0.31910070513538 1106 90-91 0.5206051254429624 1106 92-93 0.46245824275688463 1106 94-95 0.3181593001201186 1106 96-97 0.4656332900795803 1106 98-99 0.6087403024659075 1106 100-101 0.2472815616114623 1107 1 0.3137738182796994 1107 2 0.3327296072862893 1107 3 0.37923962360454766 1107 4 0.3061922507152701 1107 5 0.3840237988958819 1107 6 0.3198320760487334 1107 7 0.4409608725576817 1107 8 0.32710000008817275 1107 9 0.4782625071108413 1107 10-11 0.355488188924447 1107 12-13 0.4881216611947323 1107 14-15 0.4159096247593297 1107 16-17 0.5610509998646549 1107 18-19 0.5810281809745561 1107 20-21 0.4499476655946495 1107 22-23 0.5652491944263929 1107 24-25 0.479680494372964 1107 26-27 0.6093250510224664 1107 28-29 0.664866227026657 1107 30-31 0.8523250956449999 1107 32-33 0.707000011385226 1107 34-35 0.6820153268443079 1107 36-37 0.7221206498242694 1107 38-39 0.7238814470673631 1107 40-41 0.9955151675993648 1107 42-43 0.890690689248899 1107 44-45 0.7635557307273189 1107 46-47 0.8350778473259339 1107 48-49 0.7343705598759058 1107 50-51 0.4316857997094985 1107 52-53 0.714816005454999 1107 54-55 0.6361675531245652 1107 56-57 0.6928173485711895 1107 58-59 0.6804852088037876 1107 60-61 0.7320958512327636 1107 62-63 0.7395485884753654 1107 64-65 0.7956470290291833 1107 66-67 0.8999395392810428 1107 68-69 0.7692728563174569 1107 70-71 0.989185189436725 1107 72-73 0.7952646923376108 1107 74-75 0.6662109209629143 1107 76-77 0.5073155608491682 1107 78-79 0.5457507589789437 1107 80-81 -0.11359273076676146 1107 82-83 -0.0031201733879697713 1107 84-85 0.12842730652044665 1107 86-87 -0.01899000932848338 1107 88-89 -0.10882054645530914 1107 90-91 0.06333287452597602 1107 92-93 -0.016686902837843576 1107 94-95 0.24976346210829448 1107 96-97 0.13736021574766966 1107 98-99 0.26989757471682196 1107 100-101 -0.10194742259394118 1108 1 -0.2724761358133634 1108 2 -0.1784753580080647 1108 3 -0.23245379150434076 1108 4 -0.09813401907062058 1108 5 -0.07685981783627938 1108 6 0.018689911444823792 1108 7 -0.04681319185893784 1108 8 -0.06478365336953829 1108 9 -0.16993610466337117 1108 10-11 -0.2354479453441627 1108 12-13 -0.33898546723451517 1108 14-15 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-0.5526266469086494 2316 96-97 -0.4919567597473531 2316 98-99 -0.7391861108821338 2316 100-101 -0.2191740062000811 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2204.0 3 308.0 4 293.0 5 220.0 6 252.0 7 237.0 8 281.0 9 362.0 10 412.0 11 617.0 12 1170.0 13 2524.0 14 2176.0 15 2585.0 16 3879.0 17 7271.0 18 5824.0 19 2863.0 20 2528.0 21 2308.0 22 2198.0 23 2241.0 24 2477.0 25 2571.0 26 2808.0 27 3126.0 28 3410.0 29 3940.0 30 4665.0 31 5584.0 32 7089.0 33 9521.0 34 15306.0 35 36631.0 36 41645.0 37 72401.0 38 101980.0 39 23951.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.8386602567489 23.990173807385492 16.681810977864654 18.489354958000952 2 14.094684910712404 25.089340304863907 41.260462566675805 19.555512217747886 3 16.113797783605 33.555851309148885 31.3149468302389 19.015404077007208 4 10.416073631274314 21.33108770598096 40.49799158680457 27.75484707594016 5 9.677249304814243 41.63022707204892 34.941947047272635 13.750576575864205 6 23.069689274763718 42.36463063841445 19.373702677357834 15.191977409464002 7 22.100748686768632 37.944088219642886 25.11999536351614 14.83516773007234 8 23.038340156268937 35.739274292548515 22.786760870596044 18.43562468058651 9 26.20420592876317 18.34818641384379 23.79039374932496 31.657213908068083 10-11 21.248086867414973 30.965351555399838 28.42895264387679 19.357608933308395 12-13 23.825210748155953 30.334430979978926 29.10814014752371 16.73221812434141 14-15 21.512800283440964 30.604248189968665 29.6182635026296 18.26468802396077 16-17 16.308916211713107 33.797572556586566 31.04598707909936 18.847524152600972 18-19 15.81065363118925 30.784064998241472 36.55387079649981 16.851410574069465 20-21 17.048770046431983 30.515098626798824 36.75068330753779 15.685448019231401 22-23 17.283192753705283 29.880427179401387 36.643793660809436 16.192586406083894 24-25 16.44449772868601 31.123062005290947 36.5705266708123 15.86191359521074 26-27 15.84655537447482 31.27770831713927 36.83472042349178 16.041015884894133 28-29 15.82074451337355 30.330943137221322 37.79412520271436 16.054187146690765 30-31 16.10138568159753 31.507625266591226 36.61893163277001 15.772057419041232 32-33 15.230034579285363 32.062967232010536 36.70146550304627 16.00553268565783 34-35 15.798388779015577 31.451815198075618 36.65527739564162 16.09451862726718 36-37 15.906737327420542 31.43244090405911 36.00775557762786 16.65306619089249 38-39 15.646078596014265 32.00131703001797 36.38030960783977 15.972294766127998 40-41 15.398912825068479 31.952023647112327 36.814038093425125 15.83502543439407 42-43 15.433176237854193 31.95081377573959 35.73869256361838 16.877317422787836 44-45 15.392112909262353 32.060564943060015 35.57575444986226 16.97156769781537 46-47 16.167507085037645 31.8587991340583 35.011260680454555 16.962433100449502 48-49 16.020742574650257 32.4820201924018 34.42309879570336 17.07413843724459 50-51 16.192350368515925 32.22838068561287 34.20023514132946 17.379033804541745 52-53 15.797318225040039 32.70144060433174 34.389353308443795 17.11188786218443 54-55 16.446969957444782 32.39380370645628 34.014287338963285 17.144938997135657 56-57 16.483654496996362 32.82135755986124 32.98304107792537 17.711946865217023 58-59 15.911470312970923 32.039730757232064 34.2910433950392 17.757755534757802 60-61 16.524151780995666 32.83294577740197 33.40714512208012 17.23575731952225 62-63 16.342213859862305 32.83198840670158 33.902861169987005 16.922936563449113 64-65 16.553411540648845 32.02761502955919 34.003121238973165 17.415852190818796 66-67 16.2974122651542 32.606596581079 33.31172919521499 17.7842619585518 68-69 16.172605149845555 32.26597173074761 34.032634649346186 17.528788470060654 70-71 16.71706549201688 31.810653861925736 33.76443509663082 17.707845549426565 72-73 16.22585359724362 31.86022365801882 33.4163067990186 18.497615945718955 74-75 16.015750551890978 32.66149672568935 33.22615163014508 18.096601092274582 76-77 15.917307809289053 32.091557546793055 34.02027500588467 17.970859638033225 78-79 15.900155206011524 31.363248168331097 34.14862760340237 18.587969022255006 80-81 16.11529054009387 32.586633172473064 33.085740728498706 18.212335558934356 82-83 16.67341045048476 31.333206391520285 33.05604481046001 18.937338347534947 84-85 16.08239134832664 31.00253473190946 33.21027696937563 19.704796950388275 86-87 16.208461371654035 31.676195714837284 33.34421989622946 18.771123017279223 88-89 16.535022817826338 30.83190184698105 33.41505698836429 19.218018346828316 90-91 16.58470776515402 30.665811366672397 33.33342150859047 19.41605935958311 92-93 17.156307082646084 30.758262558016803 32.96240498074815 19.123025378588974 94-95 16.571346187930363 29.910878438613526 33.68379701764178 19.83397835581433 96-97 16.91523666173759 29.972832772088914 33.264908247779935 19.847022318393563 98-99 16.834754085350934 29.984723333619907 33.18144843959765 19.99907414143151 100-101 17.234261443578163 29.324466565815783 33.85349998413185 19.587772006474204 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8949.0 1 5253.5 2 1761.5 3 1925.5 4 1500.0 5 1333.5 6 2239.0 7 4298.0 8 6324.0 9 6273.5 10 4310.5 11 2725.5 12 2400.5 13 2352.5 14 2219.0 15 2014.5 16 1887.5 17 1837.0 18 1788.5 19 1779.0 20 1808.5 21 1854.0 22 1899.5 23 2066.5 24 2173.0 25 2223.5 26 2510.0 27 2842.0 28 2996.5 29 3558.5 30 4862.5 31 6108.0 32 8209.5 33 10956.0 34 12530.0 35 14666.0 36 19689.0 37 23348.0 38 22745.5 39 21113.5 40 18802.5 41 15790.0 42 12636.5 43 12051.5 44 12597.5 45 11967.5 46 10119.5 47 8226.0 48 9356.0 49 8414.0 50 5759.5 51 4201.5 52 2720.5 53 2066.5 54 1758.5 55 1574.0 56 1424.5 57 1398.5 58 1579.0 59 1626.0 60 1722.5 61 1852.5 62 1529.0 63 931.5 64 507.0 65 402.5 66 355.5 67 277.5 68 211.5 69 180.5 70 155.0 71 133.5 72 116.0 73 111.0 74 98.0 75 86.5 76 69.0 77 48.5 78 38.0 79 29.5 80 25.0 81 17.0 82 10.0 83 8.0 84 5.5 85 4.0 86 1.5 87 1.5 88 3.0 89 1.5 90 0.0 91 0.5 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3377533479175387 2 0.10924991378470658 3 0.11003967219760809 4 0.1208330371739285 5 0.12320231241263298 6 0.06186440901061698 7 0.06976199313963193 8 0.06870898192242993 9 0.06739271790092743 10-11 0.06581320107512445 12-13 0.06923548753103093 14-15 0.06383880504287072 16-17 0.06462856345577221 18-19 0.07463217001919113 20-21 0.07252614758478715 22-23 0.07989722610520109 24-25 0.09134872309227274 26-27 0.09253336071162498 28-29 0.08595204060411253 30-31 0.07963397330090059 32-33 0.0791074676922996 34-35 0.30116120811976954 36-37 0.4771457077946523 38-39 0.45792825308071594 40-41 0.43041833503131394 42-43 0.46042915472157064 44-45 0.47109089329574083 46-47 0.46793185964413486 48-49 0.47385504774089604 50-51 0.4718806517086423 52-53 0.4718806517086423 54-55 0.4649044523946791 56-57 0.43568339111732385 58-59 0.425679784553905 60-61 0.37460874051960835 62-63 0.451083680168903 64-65 0.47596107017530004 66-67 0.4867544351516205 68-69 0.4976794265300911 70-71 0.49438876647633484 72-73 0.48188425827206127 74-75 0.4855697975322682 76-77 0.4638514411774771 78-79 0.43594664392162435 80-81 0.4443707336592403 82-83 0.45727012106996473 84-85 0.4518734385818045 86-87 0.4530580762011568 88-89 0.4631933091667259 90-91 0.48122612626130995 92-93 0.48635955594516966 94-95 0.48438515991291603 96-97 0.48280564308711305 98-99 0.48320052229356375 100-101 0.4599026491129697 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 379863.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.090098272271845 #Duplication Level Percentage of deduplicated Percentage of total 1 72.37167918747639 18.158125429431138 2 10.651781592311243 5.345084938517307 3 4.239937885591975 3.191413746534935 4 2.4121794602761573 2.4208727883473777 5 1.5339740630377303 1.924377999436639 6 1.0397867964913754 1.56530117437076 7 0.8110546858605784 1.4244609240699935 8 0.6526209762034667 1.309945954199277 9 0.5781256557686658 1.3054706565261687 >10 4.654383682377135 24.106059289796587 >50 0.6662609644520922 11.667364286598064 >100 0.3745750619045621 16.597562805537784 >500 0.004196919461115542 0.6981464370049202 >1k 0.0052461493263944265 2.5983051784459135 >5k 0.004196919461115542 7.687508391183137 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9127 2.4027083448506437 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8068 2.1239236250964164 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6672 1.7564227102929215 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5335 1.4044537109431559 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3720 0.9793004319978519 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1950 0.5133429683859708 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1600 0.4212044868807965 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1389 0.36565814517339146 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1211 0.3187991460079029 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 884 0.23271547900164005 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 631 0.16611251951361414 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 570 0.15005409845128376 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 567 0.14926434003838226 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 492 0.12952037971584493 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 484 0.12741435728144093 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 477 0.12557158765133747 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 474 0.12478182923843598 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 462 0.12162279558683 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 458 0.12056978436962798 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 458 0.12056978436962798 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 457 0.12030653156532749 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 449 0.11820050913092352 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 431 0.11346195865351456 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 409 0.10767039695890361 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 409 0.10767039695890361 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 406 0.10688063854600212 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 402 0.10582762732880013 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 395 0.10398485769869664 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 390 0.10266859367719415 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0010530112172019912 0.0 22-23 0.0 0.0 0.0 0.0022376488365542313 0.0 24-25 0.0 0.0 0.0 0.004343671270958214 0.0 26-27 0.0 0.0 0.0 0.00842408973761593 0.0 28-29 0.0 0.0 0.0 0.018690949105335343 0.0 30-31 0.0 0.0 0.0 0.041857195883779154 0.0 32-33 0.0 0.0 0.0 0.08121349012670358 0.0 34-35 0.0 0.0 0.0 0.13820772225776135 0.0 36-37 0.0 0.0 0.0 0.22192211402531964 0.0 38-39 0.0 0.0 0.0 0.3384114799282899 0.0 40-41 0.0 0.0 0.0 0.46793185964413486 0.0 42-43 0.0 0.0 0.0 0.5879751384051619 0.0 44-45 0.0 0.0 0.0 0.7306581583360316 0.0 46-47 0.0 0.0 0.0 0.9062477788044637 0.0 48-49 0.0 0.0 0.0 1.0857861913374032 0.0 50-51 0.0 0.0 0.0 1.2769077272595646 0.0 52-53 0.0 0.0 0.0 1.461711195878514 0.0 54-55 0.0 0.0 0.0 1.670602296090959 0.0 56-57 0.0 0.0 0.0 1.9251677578495405 0.0 58-59 0.0 0.0 0.0 2.2155356009929896 0.0 60-61 0.0 0.0 0.0 2.526832042078328 0.0 62-63 0.0 0.0 0.0 2.835101075914211 0.0 64-65 0.0 0.0 0.0 3.1727227974295995 0.0 66-67 0.0 0.0 0.0 3.542329734667499 0.0 68-69 0.0 0.0 0.0 3.9634025951461447 0.0 70-71 0.0 0.0 0.0 4.441996193364449 0.0 72-73 0.0 0.0 0.0 4.915982867507497 0.0 74-75 0.0 0.0 0.0 5.406817721125774 0.0 76-77 0.0 0.0 0.0 5.9226615911525995 0.0 78-79 0.0 2.632528043004978E-4 0.0 6.548150254170583 0.0 80-81 0.0 2.632528043004978E-4 0.0 7.208519913758382 0.0 82-83 0.0 2.632528043004978E-4 0.0 7.883105224778406 0.0 84-85 0.0 2.632528043004978E-4 0.0 8.57769774892527 0.0 86-87 0.0 2.632528043004978E-4 0.0 9.298747179904334 0.0 88-89 0.0 2.632528043004978E-4 0.0 10.02624630458876 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCCAG 60 8.396051E-5 39.490143 9 GGTATCA 3225 0.0 38.30027 1 GTATGGT 50 0.0016132685 38.005653 1 GGTATGG 65 1.3253432E-4 36.543896 1 ATGTATT 55 0.0025755712 34.550594 1 GTGGTAC 55 0.0026074222 34.464127 6 ACCTGTG 130 8.447387E-9 32.807194 7 GTATCAA 4880 0.0 32.807133 1 AGAGCGT 30 0.003937037 31.688112 52-53 CTACCTC 75 3.0718982E-4 31.67138 1 GGTACTC 60 0.003993459 31.592115 8 CATATAG 60 0.003993459 31.592115 3 TGGTACT 60 0.003993459 31.592115 7 GGGTATG 80 4.5504124E-4 29.617607 7 TGGGGTC 115 3.9270126E-6 28.844975 6 CTGTGCC 165 2.7030183E-9 28.720104 9 GGTCCTA 150 3.4011464E-8 28.432905 8 ACTACCT 190 4.0381565E-10 27.435257 5 GTATAGG 420 0.0 27.146894 1 GATGGCC 70 0.00848157 27.078955 9 >>END_MODULE