FastQCFastQC Report
Fri 27 May 2016
SRR939066_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939066_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379863
Sequences flagged as poor quality0
Sequence length101
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89132.346372244730337No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76422.011777930464404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71761.8891021236603724No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55911.4718464288440833No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38201.0056257124279018No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20850.5488820969665379No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15000.39487920645074676No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14530.38250632464862333No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12090.31827264039930186No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9940.2616732874746948No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA6000.1579516825802987No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT5980.1574251769716977RNA PCR Primer, Index 26 (100% over 22bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5920.1558456601458947No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA5890.15505590173299322No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT5760.15163361527708674No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA5430.1429462727351703No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG4990.1313631493459484No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC4660.12267580680403198No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG4570.12030653156532749No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG4550.1197800259567265No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT4420.11635773950082003No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT4200.11056617780620909No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT4110.1081969025675046No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA4100.1079336497632041No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4010.10556437452449961No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC3950.10398485769869664No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3820.10056257124279017No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC3800.10003606563418917No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGAAC1006.4028427E-1042.710316
TGGGTAT853.658679E-739.0813336
GGTATCA37100.038.880621
TACCTGG1003.0464435E-837.964722
ACCTGGG1001.3055997E-633.219133
CATGGGT1900.032.4698264
CGCGAGG300.003965560331.64143464-65
GGTACTC1051.9110448E-631.637273
GTATCAA55350.031.4795041
GGATATG650.00577575429.2961851
TGGGATA1153.883597E-628.8862046
ACTAGAT1004.8696882E-528.5637781
AGTACCA1004.975903E-528.4735412
ATGGGAC2700.028.1220177
TACTCCC1205.408163E-627.682615
TCGCTCC350.00822954227.25037890-91
GGGTATG1405.836064E-727.1176597
GTCGGGA700.00842269327.1176592
TGGTACT1257.4272557E-626.5753082
CCCGCCA458.993379E-426.36439192-93
Produced by FastQC (version 0.11.5)