##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939066_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 379863 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9435112132532 33.0 31.0 34.0 30.0 34.0 2 32.18413217396798 33.0 31.0 34.0 30.0 34.0 3 32.220371555007986 34.0 31.0 34.0 30.0 34.0 4 35.89025253841517 37.0 35.0 37.0 35.0 37.0 5 35.85943616514375 37.0 35.0 37.0 35.0 37.0 6 35.93553202075485 37.0 35.0 37.0 35.0 37.0 7 35.8763159349555 37.0 35.0 37.0 35.0 37.0 8 35.86029963434186 37.0 35.0 37.0 35.0 37.0 9 37.467713359816564 39.0 37.0 39.0 35.0 39.0 10-11 37.359326915229964 39.0 37.0 39.0 34.0 39.0 12-13 37.246423052521564 39.0 37.0 39.0 33.5 39.0 14-15 38.32500401460527 40.0 38.0 41.0 33.5 41.0 16-17 38.42144536319673 40.0 38.0 41.0 34.0 41.0 18-19 38.509875928953335 40.0 38.0 41.0 34.0 41.0 20-21 38.49395703187729 40.0 38.0 41.0 34.0 41.0 22-23 38.35129243964272 40.0 37.5 41.0 34.0 41.0 24-25 38.19394887104035 40.0 37.0 41.0 34.0 41.0 26-27 37.91569855447885 40.0 37.0 41.0 33.0 41.0 28-29 37.644736391804415 39.5 36.0 41.0 33.0 41.0 30-31 37.2135282983602 39.0 35.5 40.5 33.0 41.0 32-33 36.69669723031724 38.0 35.0 40.0 31.5 41.0 34-35 36.4086802347162 38.0 35.0 40.0 31.0 41.0 36-37 36.09913310851544 38.0 35.0 40.0 30.5 41.0 38-39 35.83427841090077 38.0 35.0 40.0 30.0 41.0 40-41 35.470340622803484 38.0 35.0 40.0 29.5 41.0 42-43 35.09038653409255 38.0 35.0 40.0 27.5 41.0 44-45 34.54059094989509 38.0 34.0 40.0 24.5 41.0 46-47 33.869194946599166 37.5 33.0 40.0 22.0 41.0 48-49 33.21579753753326 37.0 33.0 40.0 18.0 41.0 50-51 32.837629619099516 37.0 33.0 40.0 14.0 41.0 52-53 32.48189478838424 36.0 32.5 40.0 9.0 41.0 54-55 32.15199953667506 36.0 32.0 39.0 7.5 40.5 56-57 31.801600840302953 35.5 31.0 39.0 2.0 40.0 58-59 31.342445829154194 35.0 31.0 39.0 2.0 40.0 60-61 30.83756380589844 35.0 30.0 38.5 2.0 40.0 62-63 30.29506558943619 35.0 28.5 38.0 2.0 40.0 64-65 29.676686068398343 35.0 28.0 38.0 2.0 40.0 66-67 29.11589178203721 34.0 27.5 37.5 2.0 39.5 68-69 28.515518752813513 34.0 26.0 37.0 2.0 39.0 70-71 27.90153950239955 33.5 26.0 36.0 2.0 39.0 72-73 27.157627881631008 33.0 24.5 35.5 2.0 38.5 74-75 26.481911899816513 32.5 22.0 35.0 2.0 38.0 76-77 24.67387321218439 30.0 20.0 33.5 2.0 36.0 78-79 25.58160442054109 31.5 20.0 35.0 2.0 36.0 80-81 25.518384259588323 32.0 20.0 35.0 2.0 36.0 82-83 24.92671700060285 31.5 16.5 35.0 2.0 36.0 84-85 24.185931506885375 31.0 7.5 34.5 2.0 35.5 86-87 23.199457435970338 30.5 2.0 34.0 2.0 35.0 88-89 22.208505434854146 29.0 2.0 34.0 2.0 35.0 90-91 21.337039932817884 29.0 2.0 34.0 2.0 35.0 92-93 20.53633941710564 28.0 2.0 33.5 2.0 35.0 94-95 19.617416015774108 26.5 2.0 33.0 2.0 35.0 96-97 18.41610922885356 25.0 2.0 33.0 2.0 35.0 98-99 16.805213985041974 11.5 2.0 32.5 2.0 34.0 100-101 12.077789887406777 2.0 2.0 25.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20842622370659925 1101 2 0.16340807656707312 1101 3 0.22138473989092944 1101 4 0.3184685861655012 1101 5 0.12770345912510805 1101 6 0.1348115248912478 1101 7 0.14347626687953863 1101 8 0.1407992759722987 1101 9 0.08484656564764492 1101 10-11 0.2580491658213404 1101 12-13 0.33989649229194185 1101 14-15 0.4272319074049946 1101 16-17 0.566030553576006 1101 18-19 0.5646102860872517 1101 20-21 0.4233881658915166 1101 22-23 0.4597327159778857 1101 24-25 0.4121945240829703 1101 26-27 0.2993555363114524 1101 28-29 0.3429158231165985 1101 30-31 0.45326405705652206 1101 32-33 0.2668045731173123 1101 34-35 0.33834862806008914 1101 36-37 0.5162621265667653 1101 38-39 0.4640946570708948 1101 40-41 0.7224166999522907 1101 42-43 0.6542161378780165 1101 44-45 0.6860830212636273 1101 46-47 0.48343343156617635 1101 48-49 0.027819007421349795 1101 50-51 -0.106578790899718 1101 52-53 -0.12003462546947219 1101 54-55 0.0528533651286196 1101 56-57 -0.053302703989331235 1101 58-59 -0.18393265190291785 1101 60-61 0.011153777557378675 1101 62-63 0.20019810472074084 1101 64-65 -0.3138707009225179 1101 66-67 -0.3241756004288199 1101 68-69 0.07650664200357227 1101 70-71 -0.044648146101938124 1101 72-73 -0.014466236283489309 1101 74-75 -0.3898683971778958 1101 76-77 0.012045664877955886 1101 78-79 0.02250111967282109 1101 80-81 0.09812819515514803 1101 82-83 -0.34424784969876754 1101 84-85 -0.4143388047438279 1101 86-87 -0.3443000113552266 1101 88-89 -0.3058885280943855 1101 90-91 -0.09870081391795793 1101 92-93 -0.5588538425068101 1101 94-95 -0.489945595661748 1101 96-97 -0.894851743526349 1101 98-99 -2.265372393836916 1101 100-101 -2.047500232022017 1102 1 0.013053121991312366 1102 2 0.057568461140434124 1102 3 0.005171476480022363 1102 4 -0.08822867156420955 1102 5 0.13510540384268666 1102 6 0.06483446818852912 1102 7 0.1735756878344148 1102 8 0.13290568917015833 1102 9 0.4756747094256397 1102 10-11 0.31055927179752274 1102 12-13 0.12214001900449745 1102 14-15 0.19894609947316866 1102 16-17 0.22861385959588887 1102 18-19 0.3259726809304695 1102 20-21 0.43670347234841955 1102 22-23 0.41648186926096287 1102 24-25 0.22176515666244967 1102 26-27 0.04360605526699146 1102 28-29 0.3777541274976741 1102 30-31 0.1804163568489443 1102 32-33 0.403112341062247 1102 34-35 0.3990527687789722 1102 36-37 0.4353596902071004 1102 38-39 0.5296605917371338 1102 40-41 1.0298022566003837 1102 42-43 0.7406358909644339 1102 44-45 0.6773427175380746 1102 46-47 0.9153410028542837 1102 48-49 1.1397222085575933 1102 50-51 1.1306658283508 1102 52-53 1.4354253330140878 1102 54-55 1.7612959522585143 1102 56-57 1.865521725159578 1102 58-59 1.4204730074437464 1102 60-61 1.515933631157285 1102 62-63 1.434957200099305 1102 64-65 0.9742452173556408 1102 66-67 0.9054485665109802 1102 68-69 0.757130481181985 1102 70-71 0.11854971252364521 1102 72-73 0.8293281480713688 1102 74-75 0.481785705302169 1102 76-77 0.6355980520734121 1102 78-79 0.9895201298334229 1102 80-81 1.0463555523058083 1102 82-83 0.7902708873273454 1102 84-85 0.48725083799626034 1102 86-87 0.8730877303687983 1102 88-89 0.22557656531760983 1102 90-91 0.20100966352113403 1102 92-93 0.377464850817784 1102 94-95 -0.37268895849360817 1102 96-97 -0.5705318484099919 1102 98-99 -0.26537239383691613 1102 100-101 -0.7119681660327242 1103 1 0.034367573697924314 1103 2 0.315230044292214 1103 3 0.09649319035075621 1103 4 0.1531293531271487 1103 5 0.2305665512646229 1103 6 0.3257277143758941 1103 7 0.3345052448424042 1103 8 0.21424192736913739 1103 9 0.3890370914154744 1103 10-11 0.19736028850050502 1103 12-13 0.35275004079098693 1103 14-15 0.12928379010497082 1103 16-17 0.24246643766415588 1103 18-19 0.3672217731599545 1103 20-21 0.3470520562264099 1103 22-23 0.4233627697693123 1103 24-25 0.3029198407583138 1103 26-27 0.5044266798131076 1103 28-29 0.4105064877010136 1103 30-31 0.16589202859912433 1103 32-33 0.4622626678596333 1103 34-35 0.6521391885320611 1103 36-37 0.46571553624922046 1103 38-39 0.23365478200583567 1103 40-41 0.5789671077260152 1103 42-43 0.6355886868976199 1103 44-45 0.8616382876179571 1103 46-47 1.1336416564490506 1103 48-49 0.8724745687609357 1103 50-51 0.9262903744655446 1103 52-53 1.3212459575602011 1103 54-55 1.247552306652132 1103 56-57 0.9706778616882694 1103 58-59 1.0417874591503562 1103 60-61 1.2363403123482861 1103 62-63 0.9455781151319833 1103 64-65 1.0510607583868712 1103 66-67 0.586530628965594 1103 68-69 0.3701297549655713 1103 70-71 0.08959183307556984 1103 72-73 0.19535937537710168 1103 74-75 -0.10485191270799987 1103 76-77 0.3864513563580907 1103 78-79 0.19567481392928698 1103 80-81 0.012495712073420151 1103 82-83 0.31583370504702657 1103 84-85 0.2716190297173924 1103 86-87 -0.03497327180985366 1103 88-89 -0.35857376147977504 1103 90-91 0.17559208908395263 1103 92-93 -0.2470631085140944 1103 94-95 -0.2609242526112574 1103 96-97 -1.1300634388239388 1103 98-99 -1.59184298207221 1103 100-101 -1.490090896606434 1104 1 0.1456280778996053 1104 2 0.22001996025860393 1104 3 0.28682932480454326 1104 4 0.19455792455572407 1104 5 0.1782976437015975 1104 6 0.09774452109858345 1104 7 0.09904305996845153 1104 8 0.1198721794699793 1104 9 0.22391104099530423 1104 10-11 0.24525944816436862 1104 12-13 0.4186744105388911 1104 14-15 0.2434434539705208 1104 16-17 0.251374000689367 1104 18-19 0.5196587479498689 1104 20-21 0.5291108797558266 1104 22-23 0.41680814792057674 1104 24-25 0.43737362227091836 1104 26-27 0.4892586125862124 1104 28-29 0.43231321039009174 1104 30-31 0.5110600958260179 1104 32-33 0.40827107122097317 1104 34-35 0.2957106171034951 1104 36-37 0.4732785614593098 1104 38-39 0.7059657063755722 1104 40-41 0.5766982001629941 1104 42-43 0.26516851883039294 1104 44-45 0.7734450103070358 1104 46-47 1.127087034600315 1104 48-49 0.9878527200214364 1104 50-51 0.4360802904319243 1104 52-53 0.5282207474761691 1104 54-55 0.8990649116941469 1104 56-57 0.7386610549655721 1104 58-59 0.6423336776377511 1104 60-61 0.3502898921802178 1104 62-63 0.6251159302580334 1104 64-65 0.201396892840652 1104 66-67 0.35594239367147296 1104 68-69 0.5012221919403608 1104 70-71 0.0755162028234686 1104 72-73 0.10624172831828105 1104 74-75 0.3957363225861208 1104 76-77 0.021409339551365036 1104 78-79 0.4944143097276026 1104 80-81 0.5887982330818211 1104 82-83 0.9349093352991247 1104 84-85 -0.011532230786809095 1104 86-87 -0.5689228516417835 1104 88-89 -0.4031535934125472 1104 90-91 -0.4562566504118486 1104 92-93 -0.33857571355611427 1104 94-95 -0.9612603870650354 1104 96-97 -1.42968528756343 1104 98-99 -1.5453723938369155 1104 100-101 -1.2868976192955106 1105 1 0.07691826918979316 1105 2 0.12760644784508912 1105 3 0.16566530364051602 1105 4 0.1487133987111946 1105 5 0.03562772103167333 1105 6 -0.04200313864907912 1105 7 0.005009685935078778 1105 8 0.07996171955952036 1105 9 -0.08866527158100723 1105 10-11 -0.08129732839240944 1105 12-13 -0.01639274452826811 1105 14-15 0.05934495987202837 1105 16-17 0.21377530309067083 1105 18-19 0.02658996488109011 1105 20-21 -0.09356718292223576 1105 22-23 0.10107351218593408 1105 24-25 0.06079649569378631 1105 26-27 -0.044831741504140155 1105 28-29 0.06163351971039788 1105 30-31 0.12854076330668107 1105 32-33 0.14136020431011076 1105 34-35 0.012894174287048088 1105 36-37 0.28483737301811374 1105 38-39 0.1416026620125308 1105 40-41 0.26579465925945556 1105 42-43 -0.4454989618370888 1105 44-45 0.09424680110883088 1105 46-47 0.028588866102147392 1105 48-49 -0.10980295763423698 1105 50-51 -0.1233702590186212 1105 52-53 -0.284066594811172 1105 54-55 -0.39765466502543134 1105 56-57 -0.5822465559420351 1105 58-59 -0.5487815034774357 1105 60-61 -0.7573332254429026 1105 62-63 -0.8962393810972777 1105 64-65 -0.5178013163575592 1105 66-67 -0.6555675778384966 1105 68-69 -1.1058799651617939 1105 70-71 -0.6836820063747417 1105 72-73 -1.4572340551575031 1105 74-75 -1.9287081218583246 1105 76-77 -2.0162300580880306 1105 78-79 -1.4360496707363737 1105 80-81 -1.0140670497834599 1105 82-83 -1.537601857212067 1105 84-85 -1.9664528657074456 1105 86-87 -2.0250604177793505 1105 88-89 -2.0707197309786842 1105 90-91 -1.4530739072291041 1105 92-93 -1.3591210941014928 1105 94-95 -1.08565192145657 1105 96-97 -1.2557170335951753 1105 98-99 -1.46052908899361 1105 100-101 -1.3545370169349074 1106 1 0.04487866337969493 1106 2 0.11074595557476385 1106 3 -0.11332680322043842 1106 4 0.1441129596845272 1106 5 0.008258611695346474 1106 6 0.07637059447875316 1106 7 0.1425091021230216 1106 8 0.1440876361444552 1106 9 0.17355975293909864 1106 10-11 0.0568909860254081 1106 12-13 0.0010865885248350082 1106 14-15 -0.20572906749708153 1106 16-17 0.029640979612082674 1106 18-19 -0.29748410919298607 1106 20-21 0.0333185300290495 1106 22-23 0.06799472091042702 1106 24-25 0.04841968003828612 1106 26-27 -0.04476168405703618 1106 28-29 -0.42762433839991587 1106 30-31 -0.30103982610997804 1106 32-33 0.06545296037007375 1106 34-35 0.22659274044463018 1106 36-37 -0.011108636042642672 1106 38-39 0.4123513425972831 1106 40-41 0.2869870370092116 1106 42-43 -0.013816649007232229 1106 44-45 0.32159489321102086 1106 46-47 0.3014976513060148 1106 48-49 0.1861196989441538 1106 50-51 0.292981149136061 1106 52-53 0.5574401073512618 1106 54-55 0.5401109694615158 1106 56-57 3.628504378632158E-4 1106 58-59 -0.10985992111322673 1106 60-61 0.3505318906189352 1106 62-63 0.6526256882595973 1106 64-65 0.7095467757446343 1106 66-67 0.7244123390266672 1106 68-69 0.3345945572799387 1106 70-71 -0.13529566290642592 1106 72-73 0.020230799356532714 1106 74-75 -0.03071176484557725 1106 76-77 -0.20777879471873462 1106 78-79 0.06275935266280896 1106 80-81 0.44346646102873777 1106 82-83 -0.1801804383152401 1106 84-85 0.34957627819055404 1106 86-87 0.2912723083894413 1106 88-89 0.027033760217427982 1106 90-91 0.49012117940860733 1106 92-93 0.133055824304936 1106 94-95 0.059005030577427675 1106 96-97 -0.9720194015368904 1106 98-99 -0.8664652900117797 1106 100-101 -1.4354300158606943 1107 1 0.2504030289759207 1107 2 0.28698669215683736 1107 3 0.021271594863250698 1107 4 0.1688827777846882 1107 5 0.16416848518887406 1107 6 0.22713786747822695 1107 7 0.30976713041854964 1107 8 0.4154103399396476 1107 9 0.2137349157506847 1107 10-11 0.3087623835851119 1107 12-13 0.27996599566608893 1107 14-15 0.17829668293334322 1107 16-17 0.3131352531355489 1107 18-19 0.5059405483412647 1107 20-21 0.3074670441393792 1107 22-23 0.3446359365702776 1107 24-25 0.2519528786309948 1107 26-27 0.2920374775568604 1107 28-29 0.32922123387345437 1107 30-31 0.46458260071838 1107 32-33 0.33899514167107014 1107 34-35 -0.0947786196871192 1107 36-37 0.10987347339668219 1107 38-39 0.3693316554949533 1107 40-41 0.5591443841160242 1107 42-43 0.36745814701043145 1107 44-45 0.42418865022875707 1107 46-47 0.35149016571577363 1107 48-49 0.02577835602927081 1107 50-51 0.08674565246715105 1107 52-53 0.08522661831765532 1107 54-55 -0.43443802372659945 1107 56-57 -0.5204583188113325 1107 58-59 -0.36375242803740093 1107 60-61 -0.7377531606573591 1107 62-63 -0.9514202732644712 1107 64-65 -1.0221060425800914 1107 66-67 -1.2320028118079769 1107 68-69 -1.3231418002318485 1107 70-71 -0.7783976915013859 1107 72-73 -0.694306216887199 1107 74-75 -1.4527568280988135 1107 76-77 -1.4585123825621373 1107 78-79 -1.282004476965156 1107 80-81 -0.8086968745502716 1107 82-83 -1.3589849895541022 1107 84-85 -1.514702485189943 1107 86-87 -1.567729113872705 1107 88-89 -1.735306235291219 1107 90-91 -0.8747302316251542 1107 92-93 -0.6143878466285209 1107 94-95 -1.5336478626031962 1107 96-97 -1.3380610214186177 1107 98-99 -1.9379751335629436 1107 100-101 -1.0338056427788764 1108 1 -0.1350536532378932 1108 2 -0.10184139554377225 1108 3 -0.016541031380832294 1108 4 0.09776742283224849 1108 5 0.04753548360967841 1108 6 0.04095401937510701 1108 7 -0.029995622528673493 1108 8 0.009424075295335399 1108 9 0.17182371813049713 1108 10-11 0.03535902686984116 1108 12-13 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1.4194696329418104 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 8.0 5 41.0 6 138.0 7 172.0 8 328.0 9 523.0 10 894.0 11 1400.0 12 2045.0 13 2658.0 14 3356.0 15 4463.0 16 6013.0 17 8455.0 18 7695.0 19 5147.0 20 4723.0 21 5054.0 22 5683.0 23 6158.0 24 6857.0 25 7745.0 26 8481.0 27 9575.0 28 11231.0 29 13265.0 30 15364.0 31 18005.0 32 21221.0 33 26739.0 34 36846.0 35 48005.0 36 43978.0 37 37341.0 38 10087.0 39 169.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.816997125221945 25.11546672867168 17.641308024012854 17.426228122093516 2 14.860884055567404 26.199708842398444 40.117621352961464 18.821785749072692 3 16.634681450944157 33.69135714718201 32.078407215232865 17.595554186640975 4 10.774674027215076 22.656852602122342 39.86595167205019 26.702521698612397 5 9.376801636379431 42.292616022092176 34.964184455974916 13.366397885553477 6 23.037252904336565 43.01787749794005 18.567483540118413 15.377386057604978 7 21.68834553510081 38.67631224941624 24.614663707705144 15.020678507777804 8 23.471620031432387 36.163827485172284 22.303040833142475 18.061511650252854 9 25.726117047461848 18.274746421736257 23.629308461208385 32.36982806959351 10-11 21.469845707531398 31.288385549527064 27.81384341196695 19.42792533097459 12-13 24.085262318256845 30.450846752645035 28.938722644742974 16.52516828435515 14-15 21.524292406574503 30.889811154855124 29.61395140070789 17.971945037862483 16-17 16.17117057824696 33.95716786668597 31.161364204762343 18.71029735030472 18-19 15.675256605670992 30.42465309861713 37.19038179554207 16.7097085001698 20-21 16.90083003609196 29.9307908377494 37.6252754282465 15.54310369791214 22-23 17.175139458173078 29.515246286161062 37.650679323861496 15.65893493180436 24-25 16.34117608345125 30.77995327256573 37.4615814933035 15.417289150679522 26-27 15.775610834831735 30.770911599082794 37.89966539335368 15.553812172731796 28-29 15.708426459012856 29.887485751441968 38.79754016579662 15.606547623748563 30-31 15.968388603259594 30.89297983746772 37.59697575178419 15.541655807488489 32-33 15.396077006710316 31.552033233034017 37.638438068461525 15.413451691794148 34-35 15.876751983507956 30.929428320557527 37.77991914580478 15.413900550129734 36-37 15.926544723956832 30.822461578566944 37.00043579180474 16.25055790567148 38-39 15.734486222335734 31.183558693468527 37.50478871088561 15.577166373310128 40-41 15.466960132059427 31.20851751709322 37.87915088105437 15.445371469792985 42-43 15.589614383163127 31.07233968612522 36.86567517830204 16.472370752409613 44-45 15.596822981521798 31.38503579016093 36.33405554253882 16.684085685778445 46-47 16.381327976706043 31.206741768266195 35.669398511482434 16.742531743545324 48-49 16.25617838503103 31.73218551937923 35.099676848250304 16.91195924733943 50-51 16.35240739058841 31.70010648617983 34.67658607150238 17.27090005172938 52-53 16.084088210749663 32.179891171290706 34.82545022810856 16.910570389851078 54-55 16.563866446587323 31.86846310380321 34.389635210589084 17.178035239020385 56-57 16.636019612359735 32.36170982921452 33.267300668004864 17.734969890420878 58-59 16.12016211513123 31.84174951658995 34.486716589421974 17.551371778856847 60-61 16.693234066470712 32.785048894107305 33.28274338787658 17.238973651545404 62-63 16.57441764652457 32.634434573835854 33.93649494089262 16.854652838746958 64-65 16.65852756391609 31.88360775930721 33.99547457111755 17.462390105659157 66-67 16.493222378130486 32.32605771179904 33.31117563899719 17.869544271073284 68-69 16.306345203055976 32.05325316049136 33.880309388541676 17.760092247910997 70-71 16.817554796215468 31.484065265807054 33.85137234048155 17.84700759749593 72-73 16.349447037484566 31.497671528945965 33.354393557677376 18.798487875892096 74-75 16.15345532468285 32.206742957329354 33.01611370415124 18.623688013836567 76-77 15.746827238979973 31.642774904560294 34.26581901627942 18.344578840180315 78-79 15.892085156574993 30.7231358176811 34.23284309853667 19.15193592720724 80-81 15.93282270728333 32.04268409060695 33.12821417336101 18.89627902874871 82-83 16.241513387721362 30.875736778786035 33.32227671555271 19.56047311793989 84-85 15.59970305083675 30.63091167078657 33.42784109007721 20.341544188299466 86-87 15.681981070427288 31.211529543202847 33.36732435338013 19.73916503298973 88-89 15.862341406312963 30.305448501559678 33.60805509937415 20.224154992753213 90-91 15.774510568083185 30.12395815305919 33.43501345533186 20.66651782352576 92-93 16.11225626080982 30.43886875004936 32.88080176274078 20.568073226400045 94-95 15.398861430122743 29.498042054690842 33.670472868472146 21.432623646714273 96-97 15.545209720346545 29.57066100146632 33.32398785878067 21.56014141940647 98-99 15.52971868731276 29.6132542870832 32.94472729570212 21.912299729901914 100-101 15.689340630701068 28.225965677099374 34.30091901553981 21.783774676659743 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7960.0 1 4829.0 2 1810.5 3 1865.0 4 1452.0 5 1260.0 6 2190.5 7 4239.5 8 5991.5 9 6078.5 10 4409.0 11 2725.5 12 2304.0 13 2237.0 14 2097.5 15 1893.0 16 1800.0 17 1738.5 18 1673.0 19 1719.0 20 1757.5 21 1826.0 22 1894.5 23 2021.5 24 2094.0 25 2227.5 26 2570.5 27 2891.0 28 3107.0 29 3710.5 30 4932.5 31 6248.5 32 8429.0 33 11138.5 34 12786.0 35 14988.0 36 19519.5 37 22828.0 38 22640.5 39 21323.0 40 19294.5 41 16341.5 42 13328.5 43 12471.0 44 12564.0 45 11934.5 46 10370.5 47 8844.5 48 9039.5 49 8115.5 50 6266.5 51 4616.0 52 3070.0 53 2168.5 54 1664.0 55 1426.0 56 1246.5 57 1240.5 58 1408.0 59 1502.0 60 1596.5 61 1651.5 62 1405.0 63 898.5 64 514.5 65 365.0 66 275.5 67 202.0 68 149.0 69 114.0 70 79.5 71 67.0 72 66.0 73 64.5 74 67.5 75 56.5 76 39.0 77 32.0 78 26.0 79 19.0 80 14.5 81 9.0 82 4.0 83 4.0 84 3.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36829067321639647 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.948792064507467E-4 16-17 0.0021060224344039823 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.316264021502489E-4 26-27 0.0036855392602069695 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011451496987071654 36-37 0.024877390006397045 38-39 0.016716553073081612 40-41 0.008950595346216925 42-43 0.007897584129014935 44-45 0.002632528043004978 46-47 0.005791561694610952 48-49 0.0027641544451552274 50-51 3.948792064507467E-4 52-53 0.0 54-55 0.0 56-57 1.316264021502489E-4 58-59 0.0025009016408547293 60-61 0.003027407249455725 62-63 9.213848150517424E-4 64-65 0.0025009016408547293 66-67 0.002632528043004978 68-69 0.004475297673108463 70-71 2.632528043004978E-4 72-73 0.0 74-75 0.0 76-77 0.08002885250735134 78-79 0.011846376193522402 80-81 6.581320107512445E-4 82-83 0.0 84-85 0.0 86-87 0.09240173430947474 88-89 0.37368735570455663 90-91 0.3655265187712412 92-93 0.0 94-95 1.316264021502489E-4 96-97 0.0 98-99 2.632528043004978E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 379863.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.427061861776483 #Duplication Level Percentage of deduplicated Percentage of total 1 73.24512361784072 18.624082893043017 2 10.182424317720628 5.1781826605907915 3 4.098852859568476 3.126653556677012 4 2.3232699714250216 2.3629571714012685 5 1.5012216838530668 1.9085828311786093 6 1.1129746966496874 1.6979805877382108 7 0.8303308899656272 1.4779012433429948 8 0.6532902638008862 1.328900156108913 9 0.5114506978092517 1.1704219679200132 >10 4.516088955149708 24.016026830725814 >50 0.6346544084151241 11.256689911889287 >100 0.37375243301445316 16.299823883873923 >500 0.00724727709446308 1.1825315969178363 >1k 0.005176626496045058 2.6501659808931115 >5k 0.004141301196836046 7.719098727699198 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8913 2.346372244730337 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7642 2.011777930464404 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7176 1.8891021236603724 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5591 1.4718464288440833 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3820 1.0056257124279018 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2085 0.5488820969665379 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1500 0.39487920645074676 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1453 0.38250632464862333 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1209 0.31827264039930186 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 994 0.2616732874746948 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 600 0.1579516825802987 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 598 0.1574251769716977 RNA PCR Primer, Index 26 (100% over 22bp) GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 592 0.1558456601458947 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 589 0.15505590173299322 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 576 0.15163361527708674 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 543 0.1429462727351703 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 499 0.1313631493459484 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 466 0.12267580680403198 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 457 0.12030653156532749 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 455 0.1197800259567265 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 442 0.11635773950082003 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 420 0.11056617780620909 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 411 0.1081969025675046 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 410 0.1079336497632041 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 401 0.10556437452449961 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 395 0.10398485769869664 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 382 0.10056257124279017 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 380 0.10003606563418917 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0010530112172019912 0.0 22-23 0.0 0.0 0.0 0.0022376488365542313 0.0 24-25 0.0 0.0 0.0 0.004343671270958214 0.0 26-27 0.0 0.0 0.0 0.00842408973761593 0.0 28-29 0.0 0.0 0.0 0.0180328170945841 0.0 30-31 0.0 0.0 0.0 0.04080418466657716 0.0 32-33 0.0 0.0 0.0 0.07897584129014934 0.0 34-35 0.0 0.0 0.0 0.13531194141045588 0.0 36-37 0.0 0.0 0.0 0.21810494836296243 0.0 38-39 0.0 0.0 0.0 0.33327805024443025 0.0 40-41 0.0 0.0 0.0 0.46003427551511994 0.0 42-43 0.0 0.0 0.0 0.5775766526352922 0.0 44-45 0.0 0.0 0.0 0.7184169029360585 0.0 46-47 0.0 0.0 0.0 0.8925586329808378 0.0 48-49 0.0 0.0 0.0 1.0698593966772232 0.0 50-51 0.0 0.0 0.0 1.2571637669370272 0.0 52-53 0.0 0.0 0.0 1.4399928395237231 0.0 54-55 0.0 0.0 0.0 1.6486206869318676 0.0 56-57 0.0 0.0 0.0 1.8980527190065892 0.0 58-59 0.0 0.0 0.0 2.184603396487681 0.0 60-61 0.0 0.0 0.0 2.4885287590526057 0.0 62-63 0.0 0.0 0.0 2.785872801510018 0.0 64-65 0.0 0.0 0.0 3.1107267620168324 0.0 66-67 0.0 0.0 0.0 3.4686189494633592 0.0 68-69 0.0 0.0 0.0 3.8733701360753745 0.0 70-71 0.0 0.0 0.0 4.333272785188344 0.0 72-73 0.0 0.0 0.0 4.787647125411004 0.0 74-75 0.0 0.0 0.0 5.254262721033635 0.0 76-77 0.0 0.0 0.0 5.745492453858365 0.0 78-79 0.0 0.0 0.0 6.336758252317283 0.0 80-81 0.0 0.0 0.0 6.953427946391199 0.0 82-83 0.0 0.0 0.0 7.574046432529623 0.0 84-85 0.0 0.0 0.0 8.208354064491672 0.0 86-87 0.0 0.0 0.0 8.861484271961206 0.0 88-89 0.0 0.0 0.0 9.509875928953335 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGGAAC 100 6.4028427E-10 42.71031 6 TGGGTAT 85 3.658679E-7 39.081333 6 GGTATCA 3710 0.0 38.88062 1 TACCTGG 100 3.0464435E-8 37.96472 2 ACCTGGG 100 1.3055997E-6 33.21913 3 CATGGGT 190 0.0 32.469826 4 CGCGAGG 30 0.0039655603 31.641434 64-65 GGTACTC 105 1.9110448E-6 31.63727 3 GTATCAA 5535 0.0 31.479504 1 GGATATG 65 0.005775754 29.296185 1 TGGGATA 115 3.883597E-6 28.886204 6 ACTAGAT 100 4.8696882E-5 28.563778 1 AGTACCA 100 4.975903E-5 28.473541 2 ATGGGAC 270 0.0 28.122017 7 TACTCCC 120 5.408163E-6 27.68261 5 TCGCTCC 35 0.008229542 27.250378 90-91 GGGTATG 140 5.836064E-7 27.117659 7 GTCGGGA 70 0.008422693 27.117659 2 TGGTACT 125 7.4272557E-6 26.575308 2 CCCGCCA 45 8.993379E-4 26.364391 92-93 >>END_MODULE