##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939065_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 948053 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.35143815799328 33.0 31.0 34.0 31.0 34.0 2 32.50698958813484 34.0 31.0 34.0 31.0 34.0 3 32.619289216953064 34.0 31.0 34.0 31.0 34.0 4 36.003140119803426 37.0 35.0 37.0 35.0 37.0 5 36.02263164612105 37.0 35.0 37.0 35.0 37.0 6 36.16568377506321 37.0 36.0 37.0 35.0 37.0 7 36.140945706621885 37.0 36.0 37.0 35.0 37.0 8 36.17512417554715 37.0 37.0 37.0 35.0 37.0 9 37.820810650881334 39.0 38.0 39.0 35.0 39.0 10-11 37.74161623875459 39.0 37.5 39.0 35.0 39.0 12-13 37.71355240688021 39.0 37.0 39.0 35.0 39.0 14-15 38.7042786637456 40.0 38.0 41.0 34.5 41.0 16-17 38.78860991948763 40.0 38.0 41.0 35.0 41.0 18-19 39.00223510710899 40.0 38.5 41.0 35.0 41.0 20-21 39.069285683395336 40.0 39.0 41.0 35.0 41.0 22-23 39.00465058388086 40.0 38.5 41.0 35.0 41.0 24-25 38.83665101001738 40.0 38.0 41.0 35.0 41.0 26-27 38.5249099997574 40.0 37.5 41.0 34.5 41.0 28-29 38.39366839195699 40.0 38.0 41.0 34.0 41.0 30-31 38.21605490410346 40.0 37.0 41.0 34.0 41.0 32-33 37.86295228220363 40.0 35.5 41.0 34.0 41.0 34-35 37.558846393608796 40.0 35.0 41.0 33.0 41.0 36-37 37.33706765339068 39.5 35.0 41.0 33.0 41.0 38-39 37.11392875714755 39.0 35.0 41.0 33.0 41.0 40-41 37.086706122969915 40.0 35.0 41.0 33.0 41.0 42-43 36.84967981747856 39.5 35.0 41.0 32.5 41.0 44-45 36.51204521266216 39.0 35.0 41.0 31.0 41.0 46-47 36.078154385883494 39.0 35.0 41.0 30.0 41.0 48-49 35.6075994696499 39.0 35.0 41.0 28.0 41.0 50-51 35.29926280492757 39.0 35.0 40.0 27.0 41.0 52-53 35.194436386995235 38.0 35.0 40.0 27.0 41.0 54-55 35.10176646242351 38.5 35.0 40.0 27.0 41.0 56-57 34.75012209233028 38.0 34.5 40.0 25.5 41.0 58-59 34.51972516304468 38.0 35.0 40.0 25.5 41.0 60-61 33.87284255205142 37.0 34.0 40.0 22.5 41.0 62-63 33.62469661506266 37.0 33.5 40.0 22.0 41.0 64-65 33.233762774865966 36.0 33.0 39.0 22.0 40.5 66-67 32.66423765338014 36.0 33.0 39.0 20.5 40.0 68-69 32.13530783616528 35.0 32.5 38.0 19.0 39.5 70-71 31.549996150004272 35.0 31.5 37.0 15.5 39.0 72-73 30.993430219618524 35.0 31.5 36.5 10.0 38.5 74-75 30.608211777189673 35.0 31.0 36.0 6.5 37.0 76-77 28.658345577726138 32.5 28.0 34.5 2.0 36.0 78-79 29.629700027319146 34.0 29.5 35.0 2.0 36.0 80-81 29.583357681479832 34.0 30.0 35.0 2.0 36.0 82-83 29.292115525186883 34.0 29.5 35.0 2.0 36.0 84-85 28.91657111996903 34.0 29.0 35.0 2.0 35.0 86-87 28.581062451149883 34.0 29.0 35.0 2.0 35.0 88-89 27.92971120812866 34.0 28.0 35.0 2.0 35.0 90-91 27.32862403262265 33.0 26.0 35.0 2.0 35.0 92-93 27.157467989658805 33.0 26.0 35.0 2.0 35.0 94-95 26.828363498665162 33.0 25.0 35.0 2.0 35.0 96-97 26.128460117736033 33.0 25.0 35.0 2.0 35.0 98-99 25.510802138699 33.0 21.5 35.0 2.0 35.0 100-101 22.041075235245287 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17661287823011662 1101 2 0.2738013646597821 1101 3 0.3116455515909422 1101 4 0.23073704910534332 1101 5 0.28023694393738907 1101 6 0.31431427227347797 1101 7 0.2988499323329137 1101 8 0.3317714105687486 1101 9 0.2888743011709778 1101 10-11 0.3973774104356309 1101 12-13 0.3953447742580991 1101 14-15 0.5383488944217305 1101 16-17 0.5253048133411866 1101 18-19 0.4259450579219859 1101 20-21 0.526550969798123 1101 22-23 0.5472782655899877 1101 24-25 0.5707552582499389 1101 26-27 0.6212959623140932 1101 28-29 0.548614866593617 1101 30-31 0.6855344078990981 1101 32-33 0.7195915100105381 1101 34-35 0.8525763710394116 1101 36-37 0.8730514296956926 1101 38-39 0.9526975672070606 1101 40-41 0.8581310848464767 1101 42-43 0.8445908164255584 1101 44-45 0.8960927514496362 1101 46-47 0.7403167396814254 1101 48-49 0.7748233635010067 1101 50-51 0.6681587721529709 1101 52-53 0.8366888341780552 1101 54-55 0.8332665473983951 1101 56-57 0.945483016789801 1101 58-59 0.9910288660408568 1101 60-61 1.0733856063206133 1101 62-63 0.8144374620108508 1101 64-65 0.8643295263733322 1101 66-67 0.919707399185306 1101 68-69 0.9396036215607708 1101 70-71 1.0271249421813309 1101 72-73 0.6479792284795494 1101 74-75 0.6574558233653889 1101 76-77 0.6855123143749502 1101 78-79 0.9351596527673287 1101 80-81 0.8584661353776788 1101 82-83 0.8684269676414509 1101 84-85 0.9578035024406617 1101 86-87 1.2642146116707274 1101 88-89 1.340355750585335 1101 90-91 1.3510348033910091 1101 92-93 1.4382659022124997 1101 94-95 1.2253381563938177 1101 96-97 1.2725165008789574 1101 98-99 1.5789416980956226 1101 100-101 1.2601446625652493 1102 1 0.22751599809875245 1102 2 0.12022993608835719 1102 3 0.25368167638568195 1102 4 0.11431668785738935 1102 5 0.19838144311636796 1102 6 0.20635039706822056 1102 7 0.2598105234659229 1102 8 0.13595860268697635 1102 9 0.16588579542384707 1102 10-11 0.2112320902385889 1102 12-13 0.2767897660479193 1102 14-15 0.3241863328453718 1102 16-17 0.3544304291047382 1102 18-19 0.31820696268389526 1102 20-21 0.3980205921298179 1102 22-23 0.5054063444077244 1102 24-25 0.2809769331267802 1102 26-27 0.4258936634635191 1102 28-29 0.358405506987701 1102 30-31 0.4540073143030412 1102 32-33 0.42269085319608735 1102 34-35 0.4391116748160897 1102 36-37 0.4421400996464371 1102 38-39 0.46160561318406934 1102 40-41 0.5318995577528796 1102 42-43 0.545801818067595 1102 44-45 0.3712364296105619 1102 46-47 0.06381838171754595 1102 48-49 -0.010459887730512207 1102 50-51 0.0294682959624879 1102 52-53 0.015691297232244494 1102 54-55 -0.03695102238814485 1102 56-57 -0.14285852672416155 1102 58-59 -0.10581021441891636 1102 60-61 -0.3481455923657606 1102 62-63 -0.29354693864596015 1102 64-65 -0.3535686673869236 1102 66-67 -0.28002576994441597 1102 68-69 -0.34210820109932527 1102 70-71 -0.48902858819634076 1102 72-73 -0.6403623150344089 1102 74-75 -0.5771911388677822 1102 76-77 -0.4201116593524681 1102 78-79 -0.4692122684149318 1102 80-81 -0.4083236839983435 1102 82-83 -0.5907684346574023 1102 84-85 -0.6072621790043335 1102 86-87 -0.5810029581158069 1102 88-89 -0.4779119013030062 1102 90-91 -0.18771724915414367 1102 92-93 -0.2299196478696004 1102 94-95 -0.3648712032120933 1102 96-97 -0.2924670787597954 1102 98-99 -0.01771265330011218 1102 100-101 -0.1624128086991199 1103 1 0.0615176689425283 1103 2 0.04949058771742898 1103 3 0.08592896126454974 1103 4 0.030231474824809368 1103 5 0.08672730777801974 1103 6 0.004345384536890151 1103 7 0.002187298737432286 1103 8 0.027395528334430708 1103 9 0.04199272942552312 1103 10-11 0.1287237360197082 1103 12-13 -0.043260359265367754 1103 14-15 0.11863077728982319 1103 16-17 0.13917353687416067 1103 18-19 0.1339024514056959 1103 20-21 0.19607823624009058 1103 22-23 0.2817012483342083 1103 24-25 0.15844142769653757 1103 26-27 0.21881346296226667 1103 28-29 0.2356089322174526 1103 30-31 0.31137991246511376 1103 32-33 0.3687748130958397 1103 34-35 0.23428711341258435 1103 36-37 0.40502648226966187 1103 38-39 0.5636941679042025 1103 40-41 0.4967074107186278 1103 42-43 0.38265436610435444 1103 44-45 0.4405242324509899 1103 46-47 0.4885573123775302 1103 48-49 0.8015597446838498 1103 50-51 0.9236725566141146 1103 52-53 0.857096894558893 1103 54-55 0.790022661822384 1103 56-57 0.7044681232340722 1103 58-59 0.7824521080539384 1103 60-61 0.7568564961889592 1103 62-63 0.6956656762245501 1103 64-65 0.7781355932647003 1103 66-67 0.8181885157698687 1103 68-69 0.8620375800201359 1103 70-71 0.7619634752957225 1103 72-73 0.7608490467032709 1103 74-75 0.6949057700712338 1103 76-77 0.6892868368881331 1103 78-79 0.7150713573160594 1103 80-81 0.44393195509939787 1103 82-83 0.43646631889314236 1103 84-85 0.2713301351142974 1103 86-87 0.6816014696202011 1103 88-89 0.8232576893402701 1103 90-91 0.7348600273705017 1103 92-93 0.7077369937344109 1103 94-95 0.5387106681329357 1103 96-97 0.42148446677069984 1103 98-99 0.7523479147867747 1103 100-101 0.5173595388363843 1104 1 0.1218127865815859 1104 2 0.1566917301082853 1104 3 0.16425753463596493 1104 4 0.08463783957389381 1104 5 0.13487092262910494 1104 6 0.037025923092585344 1104 7 0.03497663642273352 1104 8 -0.0715164803695032 1104 9 -0.039817416093313795 1104 10-11 0.11019388425852128 1104 12-13 -0.006571252778222458 1104 14-15 0.3050002863337511 1104 16-17 0.23943873475257504 1104 18-19 0.13474904463295445 1104 20-21 0.16366289556281544 1104 22-23 0.2515026899226811 1104 24-25 0.16021281352545458 1104 26-27 0.2004230100858848 1104 28-29 0.1650084970209278 1104 30-31 0.19071827775819372 1104 32-33 0.20509672252056532 1104 34-35 0.3784527600874057 1104 36-37 0.23906700994543684 1104 38-39 0.12018811595261525 1104 40-41 0.2245905876532106 1104 42-43 0.14794744375973323 1104 44-45 0.1754861527556315 1104 46-47 0.44370487119706326 1104 48-49 0.1570235009737999 1104 50-51 0.33360727713635896 1104 52-53 0.1477095696574935 1104 54-55 0.16992882257309816 1104 56-57 0.27995431934449755 1104 58-59 0.3478165851381192 1104 60-61 0.27205097463313166 1104 62-63 0.14757986091106545 1104 64-65 0.1254734145621299 1104 66-67 0.12787843825049094 1104 68-69 0.14090674906678657 1104 70-71 0.47914693782802686 1104 72-73 0.19922793967212726 1104 74-75 0.12186755437695851 1104 76-77 0.2919592155091024 1104 78-79 0.06712494088739263 1104 80-81 0.1857516205420744 1104 82-83 0.4393256960181269 1104 84-85 0.3540888730443932 1104 86-87 0.28493004299161484 1104 88-89 0.36201057931715397 1104 90-91 0.32709448949480446 1104 92-93 0.44809807084579134 1104 94-95 0.46274507585767566 1104 96-97 0.5490888459356675 1104 98-99 0.36469321602436366 1104 100-101 0.3599985977239122 1105 1 0.1673457761638275 1105 2 0.15777033686404707 1105 3 0.15595489336478607 1105 4 0.13857870035469944 1105 5 0.1800987454219083 1105 6 0.19983240525611023 1105 7 0.21751899146850917 1105 8 0.2217698502629304 1105 9 0.13557982688258363 1105 10-11 0.19407792087853437 1105 12-13 0.23615735923903713 1105 14-15 0.26287411565080276 1105 16-17 0.3341088389302058 1105 18-19 0.2917336810635547 1105 20-21 0.23229122345927067 1105 22-23 0.14082974453700814 1105 24-25 0.13201119298457087 1105 26-27 0.11006733293130821 1105 28-29 0.18170059425337826 1105 30-31 0.16255611844007944 1105 32-33 0.08446902600367423 1105 34-35 0.23620820573614765 1105 36-37 0.19280267236783288 1105 38-39 0.17477087929051294 1105 40-41 0.14204823260744348 1105 42-43 0.24428544228651816 1105 44-45 0.3074813121789717 1105 46-47 0.18276095874836784 1105 48-49 0.20501360936062696 1105 50-51 0.343982947977139 1105 52-53 0.39462633278492376 1105 54-55 0.5239275598139699 1105 56-57 0.6117363881648785 1105 58-59 0.44550415748368977 1105 60-61 0.5097864265956673 1105 62-63 0.4431181119896763 1105 64-65 0.20496936320993342 1105 66-67 -0.03245250987115611 1105 68-69 0.07923148862271745 1105 70-71 0.14546020732186804 1105 72-73 0.028195107934781305 1105 74-75 0.07780778377820141 1105 76-77 0.15504455698021857 1105 78-79 0.5103691795488636 1105 80-81 0.3131587551311519 1105 82-83 0.3166276006798121 1105 84-85 0.5420321541135316 1105 86-87 0.49891246959373703 1105 88-89 0.5294787472641147 1105 90-91 0.4097078747415708 1105 92-93 0.4449780034986368 1105 94-95 0.22930300493270295 1105 96-97 0.30111189128976434 1105 98-99 0.501305338512001 1105 100-101 0.49720526930131115 1106 1 0.09111434632936977 1106 2 0.079744598155159 1106 3 0.1257322748907299 1106 4 0.08143027313782625 1106 5 0.03548614229571001 1106 6 0.06579318625853858 1106 7 0.08454394043315006 1106 8 0.12298228153282764 1106 9 0.05319435221648661 1106 10-11 0.1696902210210922 1106 12-13 0.22884663709491093 1106 14-15 0.1638680013415339 1106 16-17 0.1584428957818531 1106 18-19 0.05690397277223269 1106 20-21 0.226896500341617 1106 22-23 0.17086423996990874 1106 24-25 0.2361164971851153 1106 26-27 0.18946456932312117 1106 28-29 0.11703221233505445 1106 30-31 0.36350629501497167 1106 32-33 0.2511596995710761 1106 34-35 0.23538865981425516 1106 36-37 0.22366354327196802 1106 38-39 0.2675906636257608 1106 40-41 0.28778398616749 1106 42-43 0.38913397529931615 1106 44-45 0.3101765006993702 1106 46-47 0.5292612493146436 1106 48-49 0.4478022261161172 1106 50-51 0.3437507432992746 1106 52-53 0.4933002826485833 1106 54-55 0.4551775908573745 1106 56-57 0.35000384325127243 1106 58-59 0.32031230923379184 1106 60-61 0.24758450538135435 1106 62-63 0.6295157606379149 1106 64-65 0.837694204391866 1106 66-67 0.8896850397362073 1106 68-69 0.3988848206977522 1106 70-71 0.2742698541224158 1106 72-73 0.33249064612810386 1106 74-75 0.35593310973396797 1106 76-77 0.5831840387291791 1106 78-79 0.7831066119363292 1106 80-81 0.5981657833583007 1106 82-83 0.43728028208505876 1106 84-85 0.36752350914579424 1106 86-87 0.6927549479006068 1106 88-89 0.8087328114668466 1106 90-91 0.927398270160058 1106 92-93 0.7509399517331374 1106 94-95 0.7394784125947105 1106 96-97 0.8660348291383819 1106 98-99 0.7473094312746618 1106 100-101 0.6838792042641337 1107 1 0.03442844049506277 1107 2 0.028947293999259216 1107 3 0.11005648437339488 1107 4 0.10533051274218508 1107 5 0.09968712668011648 1107 6 0.12647328493304855 1107 7 0.07313617630770608 1107 8 0.09840099900034005 1107 9 0.14054017330403212 1107 10-11 0.15825589976240195 1107 12-13 0.05651326835206305 1107 14-15 0.18601428983462398 1107 16-17 0.05037896981133372 1107 18-19 -0.02209641673238849 1107 20-21 0.14160738168434506 1107 22-23 0.20152769617424582 1107 24-25 0.1357004354309268 1107 26-27 0.20941900908563582 1107 28-29 0.08480719669584857 1107 30-31 0.06886906545511806 1107 32-33 0.08065936318072886 1107 34-35 0.022176190945124574 1107 36-37 0.12827681239605226 1107 38-39 0.27957796341448216 1107 40-41 0.2415385746499652 1107 42-43 0.2894078088510028 1107 44-45 0.23354441732177378 1107 46-47 0.03522775191724037 1107 48-49 -0.0764161089286759 1107 50-51 0.09104663697631565 1107 52-53 -0.06021116052505704 1107 54-55 -0.20912844174298328 1107 56-57 -0.1000781887825326 1107 58-59 -0.05819116679986536 1107 60-61 -0.008187835069293214 1107 62-63 0.06630329176878291 1107 64-65 -0.003952691964492772 1107 66-67 9.842146946041908E-4 1107 68-69 0.07914755926933736 1107 70-71 0.14171257923837643 1107 72-73 0.11416917498095458 1107 74-75 0.1140531007635559 1107 76-77 -0.10859092202528231 1107 78-79 0.019900634810870343 1107 80-81 -0.0942683844591734 1107 82-83 -0.22709715969580202 1107 84-85 0.00959649995112244 1107 86-87 -0.18298068469031037 1107 88-89 0.01133541052495346 1107 90-91 -0.08253291735690738 1107 92-93 0.07188526764499059 1107 94-95 -0.12091959030886912 1107 96-97 -0.07841177922062315 1107 98-99 -0.09307747664880495 1107 100-101 -0.2245287841215493 1108 1 -0.004278951549544274 1108 2 -0.017757766963107713 1108 3 0.01837981919222642 1108 4 0.04657467075299593 1108 5 0.06944819075059883 1108 6 0.08469663460680721 1108 7 0.023674953403677534 1108 8 0.0600135594199358 1108 9 -0.06527153467078506 1108 10-11 0.03223747964195667 1108 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-1.0124357964371953 2316 50-51 -1.0894490046855836 2316 52-53 -0.9692727771549059 2316 54-55 -1.0350948657242967 2316 56-57 -1.057045621680487 2316 58-59 -0.9150945198570639 2316 60-61 -0.880242661962825 2316 62-63 -0.9409440927738899 2316 64-65 -1.1576120598918536 2316 66-67 -0.852930109194908 2316 68-69 -0.8305380404907012 2316 70-71 -0.7266060748122882 2316 72-73 -0.8435603990017206 2316 74-75 -0.957185503477529 2316 76-77 -0.6876658999836316 2316 78-79 -0.7574941788122302 2316 80-81 -0.7214118778557683 2316 82-83 -0.7630986685260943 2316 84-85 -0.8001686315261658 2316 86-87 -0.9138467169211033 2316 88-89 -1.1456492921173158 2316 90-91 -1.1379215320507328 2316 92-93 -0.9876894421778566 2316 94-95 -0.686035615722659 2316 96-97 -1.0834786313098057 2316 98-99 -1.4556212190932918 2316 100-101 -1.107302214165447 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 14.0 5 47.0 6 124.0 7 232.0 8 432.0 9 710.0 10 1117.0 11 1819.0 12 2698.0 13 4025.0 14 4510.0 15 6086.0 16 8348.0 17 12504.0 18 12210.0 19 7534.0 20 6265.0 21 6273.0 22 6775.0 23 7395.0 24 8254.0 25 9431.0 26 10949.0 27 12722.0 28 15124.0 29 18308.0 30 22099.0 31 26745.0 32 33330.0 33 42849.0 34 61727.0 35 115915.0 36 137795.0 37 198860.0 38 133434.0 39 11384.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.76730544930346 18.037124610242397 14.595403559203996 14.600166381250146 2 13.875490083360317 28.388180829552777 34.187223710066846 23.54910537702006 3 30.235334944354374 27.60014471764764 29.581574025924713 12.58294631207327 4 10.739483973997235 16.935551071511824 42.69708550049417 29.62787945399677 5 9.392090948501824 44.601936811549564 32.485947515592486 13.520024724356128 6 23.023501850634933 39.82256266263595 16.008387716720478 21.145547770008637 7 22.635654335780806 38.767452874470095 20.848096045263294 17.748796744485805 8 22.94070057264731 25.160407698725706 19.763557522627952 32.13533420599903 9 21.73475533540846 25.44361971324388 25.52367852852108 27.297946422826573 10-11 25.23160624986156 25.347897216716785 24.847977908408076 24.572518625013583 12-13 34.787084688303295 25.144005662130702 23.30344400576761 16.765465643798393 14-15 24.888454779620396 27.423300135225503 33.623579446566936 14.064665638587156 16-17 16.318332129088063 30.905557284277478 32.52960070039608 20.24650988623838 18-19 15.26292056610789 35.86019761563837 31.93789373478789 16.93898808346584 20-21 20.579281959974814 26.953081736991496 29.109712220730277 23.357924082303416 22-23 16.949527083401456 28.2184118398444 31.34307892069325 23.488982156060896 24-25 23.311502488800663 28.014117443544468 30.76039479055363 17.91398527710124 26-27 21.820916634187533 22.969122230021902 38.951652585928194 16.258308549862374 28-29 15.503036222658437 34.493693917956065 33.477189566406096 16.5260802929794 30-31 17.74043236133167 28.914909024167574 31.63614097285255 21.708517641648204 32-33 18.61383277095268 29.709573198966726 33.08507013848382 18.59152389159678 34-35 22.752067597788937 26.57533018270813 32.245453394658 18.42714882484493 36-37 20.581794624661647 25.43998337187029 37.64530490091966 16.332917102548397 38-39 19.236999842276866 29.242976441228087 35.92885473488535 15.591168981609693 40-41 17.63382566874593 31.58784763869944 33.588151458281246 17.19017523427338 42-43 15.513665646582508 32.976409367052014 28.919016710603074 22.590908275762402 44-45 19.260133984091105 27.545165043464138 29.75228449098809 23.442416481456675 46-47 22.692631843204765 31.07387987203058 29.81222291488008 16.421265369884573 48-49 21.13754548810717 29.341754126892038 31.322504087337165 18.198196297663625 50-51 14.89080498264071 31.436688829668007 36.876266742893186 16.796239444798093 52-53 18.200900372553406 34.73960289098066 28.66061144325417 18.398885293211762 54-55 14.88972662920744 29.2646613638689 31.980068624855363 23.865543382068303 56-57 20.991336982215127 28.009035359837476 29.728664958604632 21.270962699342757 58-59 25.678324879709248 27.589738775368122 28.17248241194369 18.559453932978943 60-61 19.24821762088188 29.28133608942567 35.6279612916361 15.842484998056353 62-63 18.175172703327906 29.85475937566257 33.403128438112134 18.56693948289739 64-65 16.645491276952473 36.09098614027232 30.13065452786792 17.132868054907284 66-67 18.849704778656513 29.344131305592985 28.398737365080283 23.407426550670216 68-69 16.18120724309996 31.41333063296694 30.119633615131335 22.28582850880177 70-71 22.197282219453975 30.616220823097446 27.56913379315292 19.617363164295668 72-73 19.808228021007267 26.33950844520296 34.388952938285094 19.463310595504684 74-75 15.93998436796255 36.341586388102776 29.23169907167637 18.486730172258305 76-77 18.499541479580646 28.504906300625542 29.573007814667896 23.422544405125915 78-79 18.66332226502344 29.564728517001175 31.91154071717585 19.860408500799533 80-81 21.58438055596723 29.257608065622847 30.05231410402801 19.10569727438191 82-83 18.70019033876503 27.822369472383475 34.0093550263621 19.468085162489395 84-85 17.337901994930665 30.832822637552965 32.117297239711284 19.71197812780509 86-87 17.790609700532347 31.934610342539127 30.241687041758425 20.0330929151701 88-89 17.068195682380452 31.15674882231335 27.557338089266953 24.217717406039245 90-91 18.858855869745693 26.837736983887638 29.8888251190214 24.414582027345272 92-93 21.020871196019634 31.448769214379364 30.095416606455544 17.434942983145458 94-95 19.063016519118655 30.336225928297257 30.251789720616884 20.348967831967197 96-97 13.037773204662608 30.814521972927672 34.64684991239941 21.500854910010307 98-99 17.848551828745713 32.856929635390046 27.395401356798576 21.899117179065662 100-101 16.823305296600783 28.625419548922938 30.064227112300934 24.487048042175346 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11259.0 1 7192.0 2 4746.0 3 6131.5 4 4793.0 5 4094.5 6 6733.5 7 12676.0 8 17254.0 9 16179.5 10 10718.0 11 6218.0 12 5144.0 13 5050.5 14 4785.5 15 4167.0 16 3569.5 17 3260.0 18 3228.0 19 3250.0 20 3243.5 21 3412.5 22 3637.5 23 3892.5 24 4415.5 25 5126.5 26 5748.0 27 6400.0 28 6974.0 29 7512.5 30 8113.0 31 8680.0 32 9428.0 33 10378.0 34 11315.0 35 11772.5 36 12529.0 37 13272.0 38 12918.0 39 12472.0 40 12141.5 41 11760.0 42 11669.5 43 11676.5 44 12209.0 45 13953.5 46 21636.0 47 70501.0 48 177581.5 49 165032.5 50 61639.5 51 25407.0 52 11630.5 53 6944.5 54 5546.5 55 4807.0 56 4237.0 57 3670.5 58 3078.0 59 2892.5 60 2821.5 61 2640.0 62 2264.0 63 1371.5 64 733.5 65 583.5 66 517.5 67 454.0 68 361.0 69 274.5 70 261.5 71 256.5 72 220.5 73 298.5 74 316.5 75 188.5 76 107.5 77 95.0 78 84.5 79 54.0 80 30.0 81 21.0 82 12.5 83 4.0 84 2.5 85 1.5 86 1.5 87 1.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3412256487770199 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.383553451125622E-4 16-17 0.001898628030289446 18-19 5.2739667508040166E-5 20-21 0.0 22-23 0.0 24-25 6.328760100964819E-4 26-27 8.965743476366827E-4 28-29 0.0 30-31 0.0027952023779261284 32-33 0.0 34-35 0.00980957815649547 36-37 0.027582846106705004 38-39 0.019988333985547224 40-41 0.012868478871961799 42-43 0.00970409882147939 44-45 0.00316438005048241 46-47 0.0014767106902251245 48-49 3.1643800504824095E-4 50-51 1.5821900252412048E-4 52-53 1.0547933501608033E-4 54-55 0.0 56-57 0.0 58-59 0.003955475063103012 60-61 0.009756838488987429 62-63 0.004219173400643213 64-65 0.0019513676977974859 66-67 0.0037445163930708517 68-69 0.0035335577230386907 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.21892235982587474 78-79 1.0547933501608033E-4 80-81 0.00311164038297437 82-83 6.856156776045221E-4 84-85 0.0 86-87 0.10785262005394212 88-89 0.40388037377657154 90-91 0.388691349534256 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.001318491687701004 100-101 0.0011602726851768837 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 948053.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.58889412598418 #Duplication Level Percentage of deduplicated Percentage of total 1 76.59922231281652 20.366886122082036 2 13.16552783860251 7.001136516265988 3 4.378249467495317 3.492384346451389 4 1.9982943525875672 2.125297478940117 5 1.053351413653289 1.4003724607541537 6 0.6268086242418229 0.9999688888331779 7 0.41089634021081983 0.7647695500633906 8 0.2871548296037285 0.6108103489678852 9 0.20410884571651786 0.4884325640039999 >10 1.0842188209124262 5.3001287936169 >50 0.08218719198121065 1.4943211573744068 >100 0.07107008386251419 3.9350633081001436 >500 0.013896385112782106 2.5699579120694813 >1k 0.014690464262083938 10.250483624142396 >5k 0.003176316597207338 5.334795149873446 >10k+ 0.007146712343716511 33.86519177846109 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 61780 6.516513317293443 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 31254 3.2966511365925744 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 22955 2.421278135294124 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 19314 2.0372278765005754 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19015 2.0056895553307674 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 16336 1.723110416822688 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 15500 1.634929692749245 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14212 1.4990723092485336 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 13940 1.4703819301241599 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 13873 1.4633148146780823 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 12920 1.3627930084077577 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 12541 1.3228163404366633 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 12451 1.3133232002852162 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11713 1.2354794510433487 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11639 1.227673980252159 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 10657 1.124093273266368 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 10628 1.1210343725509018 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 10061 1.061227589596784 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 7367 0.7770662610634638 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 6944 0.7324485023516618 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6872 0.724853990230504 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6737 0.7106142800033332 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6563 0.6922608757105352 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5894 0.6216952005847775 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 5149 0.5431130959977976 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5008 0.5282405097605303 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4967 0.523915857024871 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4881 0.514844634213488 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4836 0.5100980641377645 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 4452 0.4695939994915896 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4380 0.46199948737043184 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4275 0.4509241571937434 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4118 0.4343639015962188 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3889 0.41020913387753644 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3837 0.4047242084567002 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3409 0.35957905306981786 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3097 0.32666950054480076 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2978 0.3141174596778872 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2956 0.3117969143075334 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2687 0.2834229731882078 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2630 0.27741065109229124 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2609 0.2751955850569536 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2522 0.2660188829105546 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2416 0.25483807339885006 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2253 0.23764494179122894 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2104 0.22192852087383302 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2079 0.21929153749843097 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2005 0.21148606670724104 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2001 0.21106414936717674 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1999 0.21085319069714456 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1949 0.20557922394634057 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1922 0.2027312819009064 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1860 0.1961915631299094 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1845 0.1946093731046682 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1750 0.18458883627814057 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1585 0.1671847460004873 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1563 0.16486420063013354 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1557 0.16423132462003706 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 1340 0.14134230892154764 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1167 0.12309438396376574 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1088 0.11476151649749539 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1050 0.11075330176688433 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1042 0.1099094670867557 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 968 0.10210399629556575 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 956 0.10083824427537279 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0547933501608032E-4 0.0 10-11 0.0 0.0 0.0 1.0547933501608032E-4 0.0 12-13 0.0 0.0 0.0 1.0547933501608032E-4 0.0 14-15 0.0 0.0 0.0 2.1095867003216064E-4 0.0 16-17 0.0 0.0 0.0 4.7465700757236143E-4 0.0 18-19 0.0 0.0 0.0 9.493140151447229E-4 0.0 20-21 0.0 0.0 0.0 0.0017931486952733654 0.0 22-23 0.0 0.0 0.0 0.0034808180555306505 0.0 24-25 0.0 0.0 0.0 0.005854103093392458 0.0 26-27 0.0 0.0 0.0 0.007752731123681904 0.0 28-29 0.0 0.0 0.0 0.01629655725998441 0.0 30-31 0.0 0.0 0.0 0.03644311024805575 0.0 32-33 0.0 0.0 0.0 0.0713040304708703 0.0 34-35 0.0 0.0 0.0 0.12061561959088785 0.0 36-37 0.0 0.0 0.0 0.18337582392545565 0.0 38-39 0.0 0.0 0.0 0.2656497052379983 0.0 40-41 0.0 0.0 0.0 0.3621105571102038 0.0 42-43 0.0 0.0 0.0 0.4640035947357374 0.0 44-45 0.0 0.0 0.0 0.5848829126641654 0.0 46-47 0.0 0.0 0.0 0.7513820429870481 0.0 48-49 0.0 0.0 0.0 0.8963106492991426 0.0 50-51 0.0 0.0 0.0 1.0599618375765911 0.0 52-53 0.0 0.0 0.0 1.2114301626596826 0.0 54-55 0.0 0.0 0.0 1.3888464041567297 0.0 56-57 0.0 0.0 0.0 1.6565529564275416 0.0 58-59 0.0 0.0 0.0 1.9064862407481438 0.0 60-61 0.0 0.0 0.0 2.204940019176143 0.0 62-63 0.0 0.0 0.0 2.4784479348728397 0.0 64-65 0.0 0.0 0.0 2.7195736947195988 0.0 66-67 0.0 0.0 0.0 3.0356952617627915 0.0 68-69 0.0 0.0 0.0 3.4239119542894754 0.0 70-71 0.0 0.0 0.0 3.8196704192698085 0.0 72-73 0.0 0.0 0.0 4.18425974075289 0.0 74-75 0.0 0.0 0.0 4.6164613159812795 0.0 76-77 0.0 0.0 0.0 4.978308174753943 0.0 78-79 0.0 0.0 0.0 5.5919869458774984 0.0 80-81 0.0 0.0 0.0 6.228501993032035 0.0 82-83 0.0 0.0 0.0 6.725889797300361 0.0 84-85 0.0 0.0 0.0 7.348903489572841 0.0 86-87 0.0 0.0 0.0 7.971126086832698 0.0 88-89 0.0 0.0 0.0 8.602050729231383 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACCGT 20 5.417746E-4 47.448895 18-19 GTAAGCG 410 0.0 42.819733 94-95 TCGACCG 25 0.00162465 37.959114 18-19 CGTCACT 75 3.0310595E-4 31.749811 1 GTTCGAC 30 0.0039709336 31.6351 16-17 ACCGTCT 45 2.4316081E-5 31.632599 20-21 CTTAACG 30 0.0039724717 31.632597 20-21 TTACTCG 40 4.5231535E-4 29.65556 94-95 CGGACCG 40 4.5231535E-4 29.65556 66-67 GATAGTC 85 6.446794E-4 27.911114 7 GCTTTGC 175 4.822141E-9 27.214123 1 GACCGTC 35 0.0084371045 27.113655 20-21 ATAGGAC 160 6.304799E-8 26.690002 3 GAACTCG 90 9.002786E-4 26.360498 9 GCTTATG 200 6.9667294E-10 26.193592 1 TAGGACA 225 1.03682396E-10 25.306076 4 TCATAGG 95 0.0012077889 25.065641 1 CTTTGCG 190 1.204171E-8 24.973104 2 ATGGGAG 1260 0.0 24.477604 5 CATGGGT 490 0.0 24.208618 4 >>END_MODULE