##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939064_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 933546 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.266182919749 33.0 31.0 34.0 31.0 34.0 2 32.25306733679969 34.0 31.0 34.0 31.0 34.0 3 32.3328812934767 34.0 31.0 34.0 30.0 34.0 4 35.705067559606064 37.0 35.0 37.0 35.0 37.0 5 35.80698326595583 37.0 35.0 37.0 35.0 37.0 6 36.00355847489036 37.0 37.0 37.0 35.0 37.0 7 35.982150852769976 37.0 37.0 37.0 35.0 37.0 8 36.003282109290815 37.0 37.0 37.0 35.0 37.0 9 37.61922069185664 39.0 38.0 39.0 35.0 39.0 10-11 37.5461905465826 39.0 37.5 39.0 35.0 39.0 12-13 37.49584112620053 39.0 38.0 39.0 35.0 39.0 14-15 38.49247492892691 40.0 38.0 41.0 34.0 41.0 16-17 38.57434234627967 40.0 38.0 41.0 34.5 41.0 18-19 38.64908531556024 40.0 38.0 41.0 35.0 41.0 20-21 38.628290946562885 40.0 38.0 41.0 35.0 41.0 22-23 38.68115015221532 40.0 38.0 41.0 35.0 41.0 24-25 38.53231174468103 40.0 38.0 41.0 34.5 41.0 26-27 38.267803086296766 40.0 37.5 41.0 34.5 41.0 28-29 38.166082871117226 40.0 38.0 41.0 34.0 41.0 30-31 37.9759037047987 40.0 37.0 41.0 34.0 41.0 32-33 37.604142698913606 40.0 35.5 41.0 34.0 41.0 34-35 37.13129079873943 39.0 35.0 41.0 33.0 41.0 36-37 36.73516837949067 39.0 35.0 41.0 32.0 41.0 38-39 36.49522144597053 39.0 35.0 41.0 31.5 41.0 40-41 36.478303158066126 39.0 35.0 41.0 31.5 41.0 42-43 36.22074220231247 39.0 35.0 41.0 31.0 41.0 44-45 35.847489036426694 39.0 35.0 41.0 30.0 41.0 46-47 35.41092565336898 39.0 35.0 41.0 27.5 41.0 48-49 35.08117007624691 38.5 35.0 41.0 25.5 41.0 50-51 33.795214697508214 37.0 34.0 39.0 23.0 40.5 52-53 34.078040610746555 37.5 34.0 39.5 23.5 40.5 54-55 34.70787995449608 38.0 35.0 40.0 24.0 41.0 56-57 34.527598532905714 38.0 35.0 40.5 23.0 41.0 58-59 34.36415452479042 38.0 35.0 40.0 22.5 41.0 60-61 33.82295944709741 38.0 34.0 40.0 22.0 41.0 62-63 33.58817294487899 37.0 34.0 40.0 21.0 41.0 64-65 33.190489809821905 36.5 33.5 39.5 18.5 41.0 66-67 32.582759178444334 36.0 33.0 39.0 13.0 40.5 68-69 32.11670019474134 35.5 33.0 38.5 9.0 40.0 70-71 31.411718865487078 35.0 31.5 37.5 4.5 39.0 72-73 30.888039796646336 35.0 31.5 37.0 2.0 39.0 74-75 30.53877634310468 35.0 31.0 36.5 2.0 38.0 76-77 30.102342037778534 35.0 31.0 36.0 2.0 37.0 78-79 29.641920162477263 34.5 30.5 35.0 2.0 37.0 80-81 29.068487251833332 34.0 29.0 35.0 2.0 36.0 82-83 28.647673494396635 34.0 29.0 35.0 2.0 36.0 84-85 28.27481773795828 34.0 29.0 35.0 2.0 35.5 86-87 28.073344537923145 34.0 29.0 35.0 2.0 35.0 88-89 27.488633125737778 34.0 26.5 35.0 2.0 35.0 90-91 26.91231015932798 34.0 25.0 35.0 2.0 35.0 92-93 26.461547154612628 33.0 25.0 35.0 2.0 35.0 94-95 25.875526754975116 33.0 23.0 35.0 2.0 35.0 96-97 25.13316430042012 33.0 18.5 35.0 2.0 35.0 98-99 24.5315067495335 33.0 2.0 35.0 2.0 35.0 100-101 21.677923744518214 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04383079760008002 1101 2 0.1274297887483371 1101 3 0.09826845766989578 1101 4 0.10004240227574002 1101 5 0.03959891850148267 1101 6 0.011142805614440476 1101 7 0.06824626224821628 1101 8 0.14978090514576792 1101 9 0.19240646103013148 1101 10-11 0.24659209811726157 1101 12-13 0.17222324895111996 1101 14-15 0.31857001334132207 1101 16-17 0.3731940375952476 1101 18-19 0.35319401309714493 1101 20-21 0.35797013020939517 1101 22-23 0.36094609238951847 1101 24-25 0.40330946006933033 1101 26-27 0.30502673648570067 1101 28-29 0.37242321113604504 1101 30-31 0.5856602909367155 1101 32-33 0.48712378860790295 1101 34-35 0.7032336836206383 1101 36-37 0.7340989128944884 1101 38-39 0.5798382355943446 1101 40-41 0.5820382190545388 1101 42-43 0.6766775300569634 1101 44-45 0.7977478160518316 1101 46-47 1.0547960871266255 1101 48-49 1.1503221592405595 1101 50-51 1.3117790600847954 1101 52-53 1.2078794072995862 1101 54-55 1.3069916277351723 1101 56-57 1.4146075653697352 1101 58-59 1.2782158247374937 1101 60-61 1.0995660365827646 1101 62-63 0.9817891090744695 1101 64-65 0.8847495694261056 1101 66-67 1.1441167766097493 1101 68-69 1.3159092720493533 1101 70-71 1.3382764994672804 1101 72-73 1.4029231089950933 1101 74-75 1.1103634746461388 1101 76-77 1.4397751840438282 1101 78-79 1.1849172897910982 1101 80-81 1.231239234838096 1101 82-83 1.2400493156998138 1101 84-85 1.2924768029671512 1101 86-87 1.120691463597371 1101 88-89 1.117644702618957 1101 90-91 1.521307209904549 1101 92-93 1.205413714171204 1101 94-95 1.2884091032360736 1101 96-97 1.1607531515888887 1101 98-99 1.1295583670402856 1101 100-101 0.827283274858857 1102 1 0.11371560638633582 1102 2 0.1624226150814252 1102 3 0.2834728269601854 1102 4 0.02324062008935357 1102 5 0.11672615139125497 1102 6 0.19593463175959158 1102 7 0.14559199021115177 1102 8 0.2549916306563631 1102 9 0.335086799682216 1102 10-11 0.27769403141667937 1102 12-13 0.3544290407512918 1102 14-15 0.3137730533259244 1102 16-17 0.24324218290822586 1102 18-19 0.254977421834198 1102 20-21 0.2691327837480273 1102 22-23 0.2724288488500122 1102 24-25 0.28755290941897016 1102 26-27 0.18672998961758935 1102 28-29 0.14328987260772408 1102 30-31 0.25780690571463083 1102 32-33 0.2454735854686021 1102 34-35 0.33952877114854374 1102 36-37 0.3733841742558539 1102 38-39 0.20620936857533678 1102 40-41 0.34355619447613606 1102 42-43 0.18798885192160242 1102 44-45 0.45264019576875825 1102 46-47 0.6218167769293217 1102 48-49 0.6975148031379064 1102 50-51 0.6031399482443476 1102 52-53 0.5630492604800637 1102 54-55 0.5766282994593155 1102 56-57 0.6741422778195272 1102 58-59 0.6107187239990353 1102 60-61 0.5773651036240821 1102 62-63 0.5896248313381989 1102 64-65 0.641109227595912 1102 66-67 0.582847243837783 1102 68-69 0.5081207630566809 1102 70-71 0.3532174370403318 1102 72-73 0.31235002544899615 1102 74-75 0.3194212413820239 1102 76-77 -0.1435539474611751 1102 78-79 -0.046433850126728515 1102 80-81 0.21081223740350197 1102 82-83 -0.029996843294316733 1102 84-85 0.1466527327553635 1102 86-87 0.1827203813439091 1102 88-89 0.02047092875066525 1102 90-91 0.30910418367138703 1102 92-93 -0.10406087393502261 1102 94-95 -0.14492239657217354 1102 96-97 -0.26591042864388115 1102 98-99 -0.0493417318172753 1102 100-101 -0.41627150246716127 1103 1 0.220187317474263 1103 2 0.3123695585510262 1103 3 0.2950889466604565 1103 4 0.36381760244731964 1103 5 0.2769050022875845 1103 6 0.2829740180822711 1103 7 0.14191952981088463 1103 8 0.30337088703500825 1103 9 0.3492105073555578 1103 10-11 0.3475811899578858 1103 12-13 0.36024080566639327 1103 14-15 0.41689983175710665 1103 16-17 0.4833881921086416 1103 18-19 0.5046839806884122 1103 20-21 0.5637462120891072 1103 22-23 0.5649163109101494 1103 24-25 0.6752553541690318 1103 26-27 0.7091834243610151 1103 28-29 0.7347494014420235 1103 30-31 0.6993239371572884 1103 32-33 0.5772806790377984 1103 34-35 0.5978799875954763 1103 36-37 0.5727433462182958 1103 38-39 0.5351191013364058 1103 40-41 0.5302885050139423 1103 42-43 0.5043028458037924 1103 44-45 0.3211681882756423 1103 46-47 0.3607890215466085 1103 48-49 0.33401532481104823 1103 50-51 0.21392509597270504 1103 52-53 0.3964517550262059 1103 54-55 0.2991441274968736 1103 56-57 0.5755349376412084 1103 58-59 0.49426038078552637 1103 60-61 0.3092643792097789 1103 62-63 0.07444814419981327 1103 64-65 0.14111737873884778 1103 66-67 0.0648680455545616 1103 68-69 0.15217368916879082 1103 70-71 0.1566140924040802 1103 72-73 0.17084099694667287 1103 74-75 0.2077631803462623 1103 76-77 0.514144732942885 1103 78-79 0.43832944068233815 1103 80-81 0.4195933895897852 1103 82-83 0.27484923348648493 1103 84-85 0.24407445214263745 1103 86-87 0.14897761363073414 1103 88-89 0.054484166206794526 1103 90-91 -0.05333712809945368 1103 92-93 -0.16315517821274383 1103 94-95 -0.3273649828853671 1103 96-97 -0.15298004829672962 1103 98-99 -0.23077802248407053 1103 100-101 0.07756178739340669 1104 1 0.2099960412296511 1104 2 0.26215381407499905 1104 3 0.406499623290145 1104 4 0.3845597290632554 1104 5 0.42505957045482035 1104 6 0.38054610481556495 1104 7 0.4267910572703144 1104 8 0.3423997217283201 1104 9 0.3863397592121629 1104 10-11 0.43227665502619317 1104 12-13 0.3809850811316906 1104 14-15 0.4697944751054166 1104 16-17 0.4558786225661393 1104 18-19 0.5388564158218117 1104 20-21 0.564910888443741 1104 22-23 0.6079069075453347 1104 24-25 0.46763782643456153 1104 26-27 0.6804626200610286 1104 28-29 0.5260381093818793 1104 30-31 0.5009013358462084 1104 32-33 0.5212995717213929 1104 34-35 0.4109601769234317 1104 36-37 0.489382316604285 1104 38-39 0.2584984391469831 1104 40-41 0.4482225152825734 1104 42-43 0.7456737830459303 1104 44-45 0.726760640370621 1104 46-47 0.8078714571384324 1104 48-49 0.7822481110950861 1104 50-51 0.96535408079896 1104 52-53 0.8632334090098936 1104 54-55 0.6972213370870435 1104 56-57 0.7791620521089868 1104 58-59 0.7272966476325067 1104 60-61 0.9100074513194301 1104 62-63 0.869915647867181 1104 64-65 0.8504962466677028 1104 66-67 0.8199013670453468 1104 68-69 0.8100310284921619 1104 70-71 0.7404657759559292 1104 72-73 0.9027584295587552 1104 74-75 0.8837446314785637 1104 76-77 0.9223265485200365 1104 78-79 0.7927764128103298 1104 80-81 0.4260779006835804 1104 82-83 0.47835744495618115 1104 84-85 0.6225047320087747 1104 86-87 0.7761665748483004 1104 88-89 0.6057693213823718 1104 90-91 0.8976203991766099 1104 92-93 1.216294372534481 1104 94-95 1.1722532382111766 1104 96-97 1.2999744763204895 1104 98-99 1.2651914523944505 1104 100-101 1.1733102173008092 1105 1 0.15462337626672706 1105 2 0.21650450398316679 1105 3 0.29147492624151994 1105 4 0.19953369556472467 1105 5 0.18973119925995263 1105 6 0.2122393322875169 1105 7 0.0218317371687462 1105 8 0.10253696695051673 1105 9 0.13972384127563942 1105 10-11 0.27991182056869235 1105 12-13 0.24860085451343394 1105 14-15 0.3954714960767731 1105 16-17 0.28031820002977526 1105 18-19 0.3559700658846765 1105 20-21 0.25466495438799797 1105 22-23 0.35543652082036914 1105 24-25 0.3747443305816418 1105 26-27 0.34925146915946925 1105 28-29 0.3078012959148211 1105 30-31 0.4683635538620905 1105 32-33 0.4791011661879452 1105 34-35 0.47207412155076867 1105 36-37 0.5708675761073962 1105 38-39 0.6952925413117654 1105 40-41 0.6994873696504413 1105 42-43 0.4122677177453511 1105 44-45 0.6984592681911508 1105 46-47 0.8151516997722723 1105 48-49 0.8173149199527501 1105 50-51 0.6151762511390402 1105 52-53 0.5661071854081854 1105 54-55 0.8814332271272178 1105 56-57 0.6579821850448369 1105 58-59 0.5841969566524554 1105 60-61 0.5176632636491405 1105 62-63 0.611500104682122 1105 64-65 0.599942574567045 1105 66-67 0.7059867416467966 1105 68-69 0.46013003488257453 1105 70-71 0.4525058258136916 1105 72-73 0.4803059348977392 1105 74-75 0.5451666414446592 1105 76-77 0.4767159682007609 1105 78-79 0.40261554788177634 1105 80-81 0.4906952532581563 1105 82-83 0.512790121974426 1105 84-85 0.39855732267599464 1105 86-87 0.285441264396443 1105 88-89 0.48259568926611607 1105 90-91 0.5057248768292553 1105 92-93 0.5423929207134996 1105 94-95 0.691031041142093 1105 96-97 0.8168976140866526 1105 98-99 0.7591651543333917 1105 100-101 0.5609686479813334 1106 1 0.19902229064062738 1106 2 0.4350160254562141 1106 3 0.4158961917409627 1106 4 0.41801594770670647 1106 5 0.37947096829832105 1106 6 0.3858250914275345 1106 7 0.36869725789675556 1106 8 0.327767130326599 1106 9 0.35805442506575247 1106 10-11 0.3780904699221068 1106 12-13 0.38770335038910986 1106 14-15 0.42680032370702037 1106 16-17 0.5708043370102516 1106 18-19 0.467105715929911 1106 20-21 0.5467795305148613 1106 22-23 0.5233772903376988 1106 24-25 0.568552536637732 1106 26-27 0.6552450299066379 1106 28-29 0.674941150100338 1106 30-31 0.6971073673898474 1106 32-33 0.46870789281238956 1106 34-35 0.4946208194380546 1106 36-37 0.42186007251525126 1106 38-39 0.6161203312111851 1106 40-41 0.8385286660157334 1106 42-43 0.7706294334603285 1106 44-45 0.8324103617997878 1106 46-47 0.6130083864839975 1106 48-49 0.6849097791938803 1106 50-51 0.6433386693179628 1106 52-53 0.530776441967177 1106 54-55 0.4339663546882875 1106 56-57 0.5968984215410345 1106 58-59 0.5844577197837353 1106 60-61 0.5797222280469896 1106 62-63 0.7105980771157334 1106 64-65 0.5267865424239986 1106 66-67 0.3484631931395299 1106 68-69 0.5071379109934782 1106 70-71 0.5855748482180338 1106 72-73 0.6026025130468504 1106 74-75 0.7061280059276562 1106 76-77 0.616886794660239 1106 78-79 0.6447527412190119 1106 80-81 0.6652469003232397 1106 82-83 0.5881905796403331 1106 84-85 0.6342047070621355 1106 86-87 0.6301940715913794 1106 88-89 0.9092667019849721 1106 90-91 0.8413296876429435 1106 92-93 0.710022370982248 1106 94-95 0.8135870519983541 1106 96-97 0.8727355152095306 1106 98-99 0.5866330911116364 1106 100-101 0.583180078985535 1107 1 0.06129038575429746 1107 2 0.12110173227617338 1107 3 0.0754206234106718 1107 4 0.08746982803072001 1107 5 0.18950493746476837 1107 6 0.17431477002696028 1107 7 0.17986788763283812 1107 8 0.3048510080376019 1107 9 0.2562383811671367 1107 10-11 0.23649783861821305 1107 12-13 0.24394191845809843 1107 14-15 0.23559311179167963 1107 16-17 0.3689948256436466 1107 18-19 0.3754542918379329 1107 20-21 0.3035015059525463 1107 22-23 0.1469401332956295 1107 24-25 0.25614072581985425 1107 26-27 0.16333883706629138 1107 28-29 0.28614444249647164 1107 30-31 0.39344261693256755 1107 32-33 0.4602417313281961 1107 34-35 0.5600963877683398 1107 36-37 0.5005901482926234 1107 38-39 0.41001241378238973 1107 40-41 0.45845942117642835 1107 42-43 0.6363401729429512 1107 44-45 0.6709980283793513 1107 46-47 0.824420719904424 1107 48-49 0.4430689116736275 1107 50-51 0.2986822805016587 1107 52-53 0.5604210722258642 1107 54-55 0.3724560332877118 1107 56-57 0.4406075932159723 1107 58-59 0.5619988747354014 1107 60-61 0.33877631741202663 1107 62-63 0.5179537081583305 1107 64-65 0.5361084656014228 1107 66-67 0.49334822057616634 1107 68-69 0.36600255693024764 1107 70-71 0.3869336148759892 1107 72-73 0.40608435260786635 1107 74-75 0.4487919724810929 1107 76-77 0.5054679287077981 1107 78-79 0.5116197388672994 1107 80-81 0.5340475274463117 1107 82-83 0.48032541110100624 1107 84-85 0.537601623707026 1107 86-87 0.16418936816833352 1107 88-89 0.0898193541929686 1107 90-91 -0.16746310044171508 1107 92-93 -0.07201969852389212 1107 94-95 -0.2125300109752537 1107 96-97 -0.3236585271212782 1107 98-99 -0.30479622689201946 1107 100-101 -0.4478298504631155 1108 1 -0.040270974234033474 1108 2 0.05339415895062416 1108 3 -0.012801079979567476 1108 4 0.0782646137693277 1108 5 -0.002508292750711405 1108 6 0.0014950803533366752 1108 7 -0.07458271794136095 1108 8 0.08731610848023053 1108 9 0.1565594084544557 1108 10-11 0.005431152495354752 1108 12-13 0.15340857539666786 1108 14-15 0.232350006582287 1108 16-17 0.08227368161913517 1108 18-19 0.05341649817092531 1108 20-21 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Count 2 2720.0 3 513.0 4 462.0 5 557.0 6 621.0 7 801.0 8 925.0 9 1206.0 10 1518.0 11 2196.0 12 3639.0 13 6548.0 14 5664.0 15 6276.0 16 8570.0 17 13166.0 18 10705.0 19 6588.0 20 6209.0 21 6388.0 22 6940.0 23 7524.0 24 8624.0 25 9641.0 26 10799.0 27 12622.0 28 14522.0 29 17342.0 30 21049.0 31 25308.0 32 32074.0 33 42477.0 34 61386.0 35 121945.0 36 142235.0 37 165954.0 38 128718.0 39 19097.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.259656149241465 17.526365261308076 14.145825760199262 15.068152829251192 2 13.474444851971251 27.39770540959661 34.65747371988323 24.470376018548905 3 30.023079084472453 27.53435587690869 29.564071270615134 12.878493768003723 4 10.552328855281152 16.317056820314804 43.24618561124072 29.88442871316333 5 9.460004462063875 44.06888740153426 32.9798005800683 13.491307556333556 6 23.31262629240343 38.63076255152194 16.773239142203238 21.283372013871396 7 22.36648848693027 38.83063919568252 21.28819017845848 17.514682138928734 8 22.582550646393457 24.670263756832988 20.470609171829487 32.27657642494407 9 21.649437012149562 25.12588564855364 26.406129561713183 26.818547777583618 10-11 24.932690189410852 24.824040389638444 25.12152750791021 25.121741913040495 12-13 34.36260591340817 24.912452420376326 23.63621159793027 17.088730068285233 14-15 25.22144859487476 26.691805088788477 33.849448187514405 14.237298128822355 16-17 16.41377757766772 30.596363714328596 32.82664393988122 20.16321476812247 18-19 15.221294062212035 35.640772592697026 32.199153130677225 16.93878021441372 20-21 20.251398295135957 27.10982553608014 29.05886187299181 23.57991429579209 22-23 16.656721795030567 28.136963667175436 31.459247738814966 23.74706679897903 24-25 23.173174568621164 27.645187891474084 30.617736852207912 18.563900687696844 26-27 21.676546662606004 22.76198789521771 38.94929245788848 16.612172984287803 28-29 15.609719744020861 33.97219100641664 33.68202698761281 16.736062261949698 30-31 17.468158858854675 28.909526020262106 31.879257234819768 21.74305788606345 32-33 18.460442368713505 29.513602417111485 33.20317127943188 18.822783934743136 34-35 22.537647931379187 26.592875646276052 32.01579009598745 18.853686326357312 36-37 20.341606127133826 25.248902695725643 37.77618385278595 16.633307324354583 38-39 19.479065159925273 28.960176157378744 35.60257990879872 15.958178773897266 40-41 17.519411471525714 31.53087158664357 33.862078171204175 17.08763877062654 42-43 15.437053882440857 32.84512768828059 28.874284354484246 22.84353407479431 44-45 18.854641442789955 27.653904863950977 29.828104682498445 23.66334901076062 46-47 22.482224138989302 30.886170956240637 29.969191738087776 16.66241316668228 48-49 20.81641224456829 29.153487871200497 31.351210187653123 18.67888969657809 50-51 14.791282458572832 31.15646075356388 37.18437950112402 16.86787728673927 52-53 18.09305764128984 34.422270598693835 28.88547393835751 18.599197821658812 54-55 14.689772621424984 29.03942768504156 32.524111626614776 23.74668806691868 56-57 20.801385682536385 27.790082875054434 29.75944865509025 21.64908278731894 58-59 25.19102210888839 27.660571863440385 28.459159185180443 18.689246842490785 60-61 19.429939908456014 28.762660725111445 35.87074105014693 15.936658316285609 62-63 18.07296822841048 29.617200230619783 33.678993843140866 18.63083769782887 64-65 16.55306587517662 35.81553485887583 30.60522710824689 17.026172157700657 66-67 18.559678516401878 29.335001354414448 28.667110778177076 23.438209351006602 68-69 15.900301626629323 31.209576645480986 30.62937627922008 22.26074544866961 70-71 22.043560056250307 30.24585003185758 27.78831917470826 19.922270737183855 72-73 19.658106770550052 26.05982700702333 34.84808993653047 19.43397628589615 74-75 16.053710908049094 35.700930150400886 29.85070849112286 18.394650450427164 76-77 18.38438146761053 28.33962910238988 29.94741920130596 23.328570228693632 78-79 18.68241768474675 29.10475517903443 32.39198267653554 19.820844459683276 80-81 21.644621532816856 28.964517490407516 30.24836034158294 19.14250063519269 82-83 18.74495603302866 27.22501193862229 34.51190551179407 19.51812651655498 84-85 17.8227965037781 30.126866169810956 32.4043847049982 19.645952621412743 86-87 18.00386543130784 31.48409951062994 31.05088048926239 19.46115456879983 88-89 17.42585846534211 30.49583110342503 28.027066869251815 24.051243561981043 90-91 18.980519158817106 26.31518295060215 30.379956101333118 24.324341789247626 92-93 21.52924754183644 30.64128206868102 30.692658737211033 17.136811652271504 94-95 19.496625718636846 29.345100776095485 30.898745710796195 20.259527794471477 96-97 13.749028650698214 29.672210335964344 35.42200583428875 21.156755179048687 98-99 18.585784062942597 31.830225156575636 28.198208871632207 21.38578190884955 100-101 17.63897017216381 27.147578901009446 31.221266324926955 23.992184601899794 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12321.0 1 7690.5 2 4780.5 3 6175.5 4 4636.0 5 3942.0 6 6746.0 7 12461.5 8 16932.0 9 15979.5 10 10495.5 11 6166.0 12 5291.5 13 5079.5 14 4800.5 15 4444.5 16 4146.5 17 3770.0 18 3384.0 19 3319.0 20 3249.5 21 3322.0 22 3662.0 23 3940.5 24 4319.0 25 4912.0 26 5579.0 27 6242.0 28 6684.0 29 7325.0 30 7986.5 31 8519.5 32 9341.5 33 10171.5 34 10991.5 35 11529.0 36 12277.5 37 13057.5 38 12684.5 39 12276.5 40 11963.0 41 11552.0 42 11470.5 43 11363.0 44 11754.0 45 13317.0 46 22219.5 47 66336.5 48 169142.0 49 161341.5 50 61584.0 51 25792.0 52 12260.5 53 7973.0 54 6312.5 55 5265.0 56 4505.0 57 3779.0 58 3164.0 59 2969.5 60 2880.0 61 2751.5 62 2428.5 63 1529.5 64 818.5 65 651.5 66 559.5 67 481.0 68 390.0 69 315.0 70 288.0 71 262.0 72 232.0 73 315.0 74 301.5 75 177.5 76 133.5 77 116.0 78 93.0 79 74.5 80 60.5 81 46.0 82 37.5 83 21.0 84 14.0 85 10.0 86 4.5 87 5.5 88 5.0 89 2.0 90 2.0 91 1.5 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.398909105710913 2 0.11697334678741059 3 0.11783029438292275 4 0.13079162676504424 5 0.13304111420326367 6 0.07401884856236329 7 0.08355239056243613 8 0.0808744293264606 9 0.0808744293264606 10-11 0.07846426421408265 12-13 0.08221340994444838 14-15 0.0761612175511437 16-17 0.07776799429272901 18-19 0.08746221396696038 20-21 0.0860161148995336 22-23 0.0968886375175942 24-25 0.10851098928172795 26-27 0.10947505532667913 28-29 0.10390489595585006 30-31 0.09308593256250898 32-33 0.09238966264115533 34-35 0.3444929333958905 36-37 0.5377881754086034 38-39 0.5174892292399089 40-41 0.4911380906779098 42-43 0.5183461768354211 44-45 0.5279332780602134 46-47 0.5247732838017622 48-49 0.5324858121613718 50-51 0.531146831543384 52-53 0.5301827654984328 54-55 0.5245054876781649 56-57 0.49461944028467797 58-59 0.4853001351834832 60-61 0.4335619241044362 62-63 0.5082234833634336 64-65 0.5358064840939815 66-67 0.5463576513637249 68-69 0.5619433857571025 70-71 0.5565339040604319 72-73 0.5429834202063958 74-75 0.5456613814423713 76-77 0.5227380332624209 78-79 0.49622621702626335 80-81 0.502814001666763 82-83 0.5181854991612626 84-85 0.5111692407230066 86-87 0.5142756757567383 88-89 0.523059388610738 90-91 0.5403054589704203 92-93 0.5472681581839566 94-95 0.5411624065659325 96-97 0.5430369794311153 98-99 0.5442688415996642 100-101 0.5212919341949941 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 933546.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.745588812087757 #Duplication Level Percentage of deduplicated Percentage of total 1 77.85584428344629 21.601562421064337 2 12.446764316528803 6.906856095347494 3 4.216341953263628 3.5095467037922266 4 1.8411922333186586 2.043398505186762 5 1.0062188421134421 1.395906712412731 6 0.5862816994358023 0.9760038576358676 7 0.38055882909572525 0.7391180153629701 8 0.25558839119632176 0.567316032582094 9 0.19424550534991852 0.48505103280315287 >10 1.0354402585954907 5.2651654006241815 >50 0.08050517493327937 1.5554029737417228 >100 0.06502341048814779 3.8710032429994787 >500 0.011224279191406464 2.143243063882809 >1k 0.014707676181842952 10.155866471466329 >5k 0.003870441100484987 7.518962301026286 >10k+ 0.006192705760775979 31.26559717007156 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 56599 6.062797119799131 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 33747 3.614926313218631 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 24846 2.6614649947619076 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 20727 2.2202441015225816 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18510 1.9827624991162727 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 14993 1.606026912439237 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 13785 1.4766278255169 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 13564 1.4529546481908766 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13474 1.4433139877413645 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 12634 1.3533344902125872 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 12049 1.2906701972907602 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 11544 1.2365753803240547 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11498 1.2316479316498599 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 11239 1.2039042532451534 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 11036 1.1821592080090322 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10902 1.1678053357842035 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 9759 1.045368948075403 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 9074 0.9719928102096737 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 7662 0.8207415596017764 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 7088 0.7592555696237786 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6796 0.7279769823875846 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 6771 0.725299021151609 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 6566 0.7033397390166097 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 6137 0.6573859242072699 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5086 0.5448044338468592 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 5078 0.543947486251347 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4989 0.5344139442512742 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4831 0.5174892292399089 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4578 0.4903882615318367 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4570 0.4895313139363245 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 4328 0.46360864917208156 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4274 0.4578242529023744 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4219 0.45193273818322827 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4075 0.4365076814640093 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3869 0.414441280879571 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3269 0.3501702112161586 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2979 0.31910586087884263 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2968 0.31792755793501337 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2825 0.3026096196652334 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2758 0.29543268355281904 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2378 0.2547276727659912 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2288 0.2450870123164793 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2154 0.23073314009165055 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2101 0.22505586227138247 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2091 0.22398467777699224 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2024 0.21680774166457786 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1939 0.2077026734622611 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1924 0.20609589672067577 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1815 0.19441998573182254 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1802 0.19302744588911525 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1799 0.1927060905407982 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1790 0.191742024495847 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1756 0.18809999721492032 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1733 0.18563627287782283 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1712 0.1833867854396034 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1480 0.15853530516975062 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1471 0.15757123912479942 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1266 0.1356119569898002 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1210 0.12961332382121502 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 1118 0.11975842647282513 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1066 0.11418826710199603 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1061 0.11365267485480095 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1036 0.11097471361882541 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1026 0.10990352912443521 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 942 0.10090557937155749 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0711844943902068E-4 0.0 10-11 0.0 0.0 0.0 2.1423689887804136E-4 0.0 12-13 0.0 0.0 0.0 3.2135534831706204E-4 0.0 14-15 0.0 0.0 0.0 5.355922471951034E-4 0.0 16-17 0.0 0.0 0.0 7.498291460731447E-4 0.0 18-19 0.0 0.0 0.0 8.569475955121655E-4 0.0 20-21 0.0 0.0 0.0 0.0016067767415853102 0.0 22-23 0.0 0.0 0.0 0.0033742311573291515 0.0 24-25 0.0 0.0 0.0 0.005784396269707117 0.0 26-27 0.0 0.0 0.0 0.008944390528158227 0.0 28-29 0.0 0.0 0.0 0.01751386648327988 0.0 30-31 0.0 0.0 0.0 0.038509082573327934 0.0 32-33 0.0 0.0 0.0 0.07573274375338762 0.0 34-35 0.0 0.0 0.0 0.13030959374256865 0.0 36-37 0.0 0.0 0.0 0.19200982061944458 0.0 38-39 0.0 0.0 0.0 0.27561577040660024 0.0 40-41 0.0 0.0 0.0 0.3706298350590116 0.0 42-43 0.0 0.0 0.0 0.4714818552058495 0.0 44-45 0.0 0.0 0.0 0.5888301165662967 0.0 46-47 0.0 0.0 0.0 0.7475796586349253 0.0 48-49 0.0 0.0 0.0 0.8934749867708716 0.0 50-51 0.0 0.0 0.0 1.0555987599968293 0.0 52-53 0.0 0.0 0.0 1.214455420514897 0.0 54-55 0.0 0.0 0.0 1.393182553403903 0.0 56-57 0.0 0.0 0.0 1.659746814832906 0.0 58-59 0.0 0.0 0.0 1.9234188781270554 0.0 60-61 0.0 0.0 0.0 2.239900336994642 0.0 62-63 0.0 0.0 0.0 2.527192018390095 0.0 64-65 0.0 0.0 0.0 2.7735108928751235 0.0 66-67 0.0 0.0 0.0 3.0825476195066983 0.0 68-69 0.0 0.0 0.0 3.466727938419746 0.0 70-71 0.0 0.0 0.0 3.863601793591317 0.0 72-73 0.0 0.0 0.0 4.22748316633567 0.0 74-75 0.0 0.0 0.0 4.656653234013107 0.0 76-77 0.0 0.0 0.0 5.018927830015874 0.0 78-79 0.0 0.0 0.0 5.643696186368963 0.0 80-81 0.0 0.0 0.0 6.290423824857051 0.0 82-83 0.0 0.0 0.0 6.786757160332753 0.0 84-85 0.0 0.0 0.0 7.400010283371146 0.0 86-87 0.0 0.0 0.0 8.020226105623076 0.0 88-89 0.0 0.0 0.0 8.645583613448078 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCACGC 35 3.4696532E-6 67.68094 3 GTCGTCA 45 9.6251414E-4 42.221313 1 TCACGCA 60 8.417514E-5 39.48055 4 TATCACG 60 8.417514E-5 39.48055 2 CATTCCG 50 0.0016416103 37.876938 9 GCTTATG 180 0.0 36.94365 1 GTAAGCG 325 0.0 36.584908 94-95 GGACCCG 55 0.002620732 34.433582 9 AGTACTC 300 0.0 33.16366 5 TACCTGG 285 0.0 29.922098 2 ACAGTCG 40 4.461384E-4 29.725233 72-73 TTCGCGA 40 4.4641993E-4 29.722033 48-49 TCGCGAT 40 4.4649033E-4 29.721231 50-51 GGGAATG 440 0.0 27.977285 7 TAAGGGC 70 0.008497858 27.072374 4 AAGTACT 370 0.0 26.889456 4 AAAGTAC 375 0.0 25.26755 3 GTAGGAC 170 1.15094736E-7 25.08176 3 ATGGGGA 730 0.0 24.661821 5 ACTACCT 135 1.36969065E-5 24.565674 4 >>END_MODULE