FastQCFastQC Report
Fri 27 May 2016
SRR939063_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939063_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2833916
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118090.4167025416420247No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87340.3081954440428016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63680.2247067308981635No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34870.12304528433446864No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA132200.029.8079641
GGTATCA92450.028.809891
GTACATG104050.026.6931271
TACATGG110000.025.070972
ATCAACG160200.024.4319613
CAACGCA162350.024.1375985
TCAACGC162400.024.1009884
TATCAAC163750.024.0180442
ACATGGG110950.023.8740083
AACGCAG164900.023.7546846
GCGCGAC1000.001682408723.68129
GAGTACT86250.023.00932912-13
GTATCGA1302.9998415E-421.8611156
ACGCAGA182750.021.3826227
GTACTTT94000.021.18992414-15
GTATAGG7200.021.1010061
AGTACTT91550.021.00471112-13
CGCAGAG184550.020.9683728
CATGGGG66050.020.876664
GTACACG3252.1827873E-1120.4517441
Produced by FastQC (version 0.11.5)