FastQCFastQC Report
Fri 27 May 2016
SRR939063_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939063_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2833916
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116680.411727094239914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91860.32414510521836215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66940.23621024758673156No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40370.14245305788880122No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA137850.028.5609911
GGTATCA97650.027.350491
GTACATG107750.025.1402471
TACATGG110500.023.8750422
ACATGGG110350.022.9615143
ATCAACG171650.022.8332563
TCAACGC172850.022.6198374
CAACGCA173400.022.5480885
TATCAAC175150.022.4582562
AACGCAG177450.022.0869466
GAGTACT90600.021.6837512-13
CATGGGG65600.021.1931444
AGTACTT96250.021.1504112-13
ATGGGGA39500.020.781685
AGAGTAC155400.020.29112810-11
GCACCGT6600.020.1300896
ACGCAGA195750.019.9009117
CGCAGAG196750.019.7274138
GTACTTT101300.019.5807414-15
TACTTTT110800.018.43722714-15
Produced by FastQC (version 0.11.5)