##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939063_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2833916 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.44655452031747 34.0 31.0 34.0 31.0 34.0 2 32.66214877222896 34.0 31.0 34.0 31.0 34.0 3 32.74938989017318 34.0 31.0 34.0 31.0 34.0 4 36.232417968634216 37.0 35.0 37.0 35.0 37.0 5 36.18617983031254 37.0 35.0 37.0 35.0 37.0 6 36.22137812129929 37.0 37.0 37.0 35.0 37.0 7 36.16747003086895 37.0 37.0 37.0 35.0 37.0 8 36.154328850961 37.0 37.0 37.0 35.0 37.0 9 37.88885873822654 39.0 38.0 39.0 35.0 39.0 10-11 37.8205858959828 39.0 38.0 39.0 35.0 39.0 12-13 37.74760384570326 39.0 38.0 39.0 35.0 39.0 14-15 39.09496223600135 40.0 38.0 41.0 36.0 41.0 16-17 39.03320123108801 40.0 38.0 41.0 36.0 41.0 18-19 39.04031629730733 40.0 38.5 41.0 35.5 41.0 20-21 39.10969273612909 40.0 39.0 41.0 36.0 41.0 22-23 39.06806235611782 40.0 39.0 41.0 35.0 41.0 24-25 39.000412679839485 40.0 38.5 41.0 35.0 41.0 26-27 38.86214111498012 40.0 38.0 41.0 35.0 41.0 28-29 38.698209297664434 40.0 38.0 41.0 34.5 41.0 30-31 38.49755250332049 40.0 38.0 41.0 34.0 41.0 32-33 38.36423909530134 40.0 38.0 41.0 34.0 41.0 34-35 38.31301351204482 40.0 38.0 41.0 34.0 41.0 36-37 38.202422901737386 40.0 38.0 41.0 34.0 41.0 38-39 38.06844468925684 40.0 38.0 41.0 33.0 41.0 40-41 37.89001561796468 40.0 37.0 41.0 33.0 41.0 42-43 37.714094736752955 40.0 37.0 41.0 33.0 41.0 44-45 37.492465196568986 40.0 37.0 41.0 33.0 41.0 46-47 37.19917404044439 40.0 36.5 41.0 32.0 41.0 48-49 36.889633637694274 40.0 36.0 41.0 31.0 41.0 50-51 36.61941903006299 39.0 36.0 41.0 31.0 41.0 52-53 36.37005772224724 39.0 35.0 41.0 30.0 41.0 54-55 36.10010917754796 39.0 35.0 40.5 30.0 41.0 56-57 35.8050584420992 38.5 35.0 40.0 29.0 41.0 58-59 35.47943746391918 38.0 35.0 40.0 28.5 41.0 60-61 35.09260613229185 38.0 34.0 40.0 28.0 41.0 62-63 34.67123284529252 37.0 34.0 40.0 27.0 41.0 64-65 34.23358190574456 36.5 34.0 39.0 26.0 41.0 66-67 33.85211841141375 36.0 33.0 39.0 26.0 40.0 68-69 33.37275152121658 35.5 33.0 38.5 26.0 40.0 70-71 32.91149825894628 35.0 32.5 38.0 25.0 40.0 72-73 32.43041942668731 35.0 32.0 37.0 23.5 39.0 74-75 31.919384695947233 35.0 31.5 36.5 22.5 39.0 76-77 29.956983199219735 33.0 29.5 35.0 20.0 37.0 78-79 30.961421757031612 34.0 31.0 35.0 20.0 37.0 80-81 31.001822919239665 34.0 31.0 35.0 20.0 37.0 82-83 30.720204656736474 34.0 31.0 35.0 19.0 37.0 84-85 30.34270793488586 34.0 31.0 35.0 17.5 36.0 86-87 29.782716918920674 34.0 30.0 35.0 9.5 36.0 88-89 29.24097309165127 34.0 29.0 35.0 4.5 35.0 90-91 28.77314376996354 34.0 29.0 35.0 2.0 35.0 92-93 28.463823910094725 34.0 29.0 35.0 2.0 35.0 94-95 28.10053685430337 34.0 29.0 35.0 2.0 35.0 96-97 27.498066280016765 33.0 27.0 35.0 2.0 35.0 98-99 26.647525897027293 33.0 26.0 35.0 2.0 35.0 100-101 22.946060504263357 28.5 11.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1060185225050958 1101 2 0.11422413997637193 1101 3 0.18293347650620717 1101 4 0.09911633718594715 1101 5 0.06946903328347531 1101 6 0.1194432481593779 1101 7 0.11202767401623248 1101 8 0.17944433953214656 1101 9 0.20305551381572684 1101 10-11 0.2002253999781871 1101 12-13 0.17764249704423918 1101 14-15 0.22291100410998865 1101 16-17 0.22346158605854782 1101 18-19 0.2067352889727232 1101 20-21 0.29699533008852086 1101 22-23 0.2619745098790247 1101 24-25 0.20840978981965463 1101 26-27 0.18399884442577275 1101 28-29 0.17232070508662645 1101 30-31 0.2316532326412002 1101 32-33 0.19881451839305697 1101 34-35 0.324180382521142 1101 36-37 0.31977548964287195 1101 38-39 0.3481655159336299 1101 40-41 0.21279634114475243 1101 42-43 0.2989646462747402 1101 44-45 0.37566894520136174 1101 46-47 0.2620313914744159 1101 48-49 0.29270366096073275 1101 50-51 0.31686028437275127 1101 52-53 0.21559425997541126 1101 54-55 0.2628413845168396 1101 56-57 0.24074624421985646 1101 58-59 0.2429595269414122 1101 60-61 0.2978957867471834 1101 62-63 0.25016691452466944 1101 64-65 0.22671861753488542 1101 66-67 0.25447390592912456 1101 68-69 0.2609679995769838 1101 70-71 0.22215497039872645 1101 72-73 0.22879156511519483 1101 74-75 0.26796206681511947 1101 76-77 0.3136842263256483 1101 78-79 0.41858968714798195 1101 80-81 0.377615035049633 1101 82-83 0.41195110626064846 1101 84-85 0.40786712342595166 1101 86-87 0.3472878702004287 1101 88-89 0.44585176774931057 1101 90-91 0.5460178869852008 1101 92-93 0.29175604990983217 1101 94-95 0.08802277277087 1101 96-97 0.3170876145845689 1101 98-99 0.17142592784095712 1101 100-101 0.17480134379162493 1102 1 0.1402195235241166 1102 2 0.0961474530471591 1102 3 0.17383670811963725 1102 4 0.10928082704877795 1102 5 0.10833872400910138 1102 6 0.08828002162497484 1102 7 0.11776440665671828 1102 8 0.132404803459778 1102 9 0.23297373499785579 1102 10-11 0.18089189412926743 1102 12-13 0.2004216267298844 1102 14-15 0.24729219819645465 1102 16-17 0.32443351316481994 1102 18-19 0.2111490627887136 1102 20-21 0.20135067898254277 1102 22-23 0.2790144959920511 1102 24-25 0.28351193297758925 1102 26-27 0.25981365912152654 1102 28-29 0.20054275515043685 1102 30-31 0.24221383865310742 1102 32-33 0.3294672702309924 1102 34-35 0.2726729070242442 1102 36-37 0.20551289734434874 1102 38-39 0.23458561925011168 1102 40-41 0.3428400915264831 1102 42-43 0.41115889989837484 1102 44-45 0.42478614221952427 1102 46-47 0.32504664785294324 1102 48-49 0.3353766774868703 1102 50-51 0.38158569527595176 1102 52-53 0.40085944643690397 1102 54-55 0.39798063995670674 1102 56-57 0.2573307248890089 1102 58-59 0.18389016281672355 1102 60-61 0.2743599458301347 1102 62-63 0.23690638598404945 1102 64-65 0.17998374291942554 1102 66-67 0.19474900935811235 1102 68-69 0.25866475574816405 1102 70-71 0.1395281473322072 1102 72-73 0.11300437970165689 1102 74-75 0.24708821642792245 1102 76-77 0.1440186547579323 1102 78-79 0.09422689651011495 1102 80-81 0.05561624373027385 1102 82-83 0.08232211656979516 1102 84-85 0.19558671916085402 1102 86-87 0.32055955332036845 1102 88-89 0.2244814260720105 1102 90-91 0.4584050955436858 1102 92-93 0.3934139675716821 1102 94-95 0.3010060441164555 1102 96-97 0.21775279075941611 1102 98-99 -0.09214553058011177 1102 100-101 -0.3713943660350161 1103 1 0.08917584463969064 1103 2 0.08744754758912876 1103 3 0.06909120939526758 1103 4 0.04123167513854753 1103 5 0.1034598380926326 1103 6 0.07348569067357147 1103 7 0.1473611225934235 1103 8 0.12054183107520089 1103 9 0.2209506112470052 1103 10-11 0.1708579618649324 1103 12-13 0.16259492723579427 1103 14-15 0.21178304695533257 1103 16-17 0.1506547215425087 1103 18-19 0.12355029245382809 1103 20-21 0.14373165585012515 1103 22-23 0.13821077775437374 1103 24-25 0.18300843195101635 1103 26-27 0.20869811347311895 1103 28-29 0.27905874364680017 1103 30-31 0.2789651697370914 1103 32-33 0.33621252361709253 1103 34-35 0.37626389096698176 1103 36-37 0.3188539088566671 1103 38-39 0.33974171881779114 1103 40-41 0.3789280490111011 1103 42-43 0.33936353803063213 1103 44-45 0.45851225426476816 1103 46-47 0.5276169069078449 1103 48-49 0.47363965780157713 1103 50-51 0.4829833489500146 1103 52-53 0.5316327947713901 1103 54-55 0.4786747799655515 1103 56-57 0.507741170847062 1103 58-59 0.6306500559582773 1103 60-61 0.6558878951863676 1103 62-63 0.599488614572401 1103 64-65 0.5553508210026692 1103 66-67 0.576601598234376 1103 68-69 0.5946393991713066 1103 70-71 0.38593387982181326 1103 72-73 0.32322473187358014 1103 74-75 0.3107705331150079 1103 76-77 0.20775905342022227 1103 78-79 0.4333633677776554 1103 80-81 0.40244958346553616 1103 82-83 0.4129186788603114 1103 84-85 0.4942428416515554 1103 86-87 0.49065006207510464 1103 88-89 0.40689321271693757 1103 90-91 0.4872555742630702 1103 92-93 0.27407053241470436 1103 94-95 0.15000173706694753 1103 96-97 0.08556363619754848 1103 98-99 -0.014853445465078607 1103 100-101 -0.15343517148796693 1104 1 0.06762401263783602 1104 2 0.07844912901099121 1104 3 0.1056491540717488 1104 4 0.05210295862932668 1104 5 0.12679572880057322 1104 6 0.13312012648560767 1104 7 0.13591336170001256 1104 8 0.0529502370861934 1104 9 0.16220301679656757 1104 10-11 0.1640124071496203 1104 12-13 0.15139161229237175 1104 14-15 0.17419177645284378 1104 16-17 0.1909211645762241 1104 18-19 0.1320294541232343 1104 20-21 0.1836967376159251 1104 22-23 0.21298514590279183 1104 24-25 0.16862762483382454 1104 26-27 0.21966079716615639 1104 28-29 0.22927798206185912 1104 30-31 0.2853026064664803 1104 32-33 0.2173841735021398 1104 34-35 0.26096856302793725 1104 36-37 0.27309796051929425 1104 38-39 0.33534092877825117 1104 40-41 0.30741158690388204 1104 42-43 0.2808861768994291 1104 44-45 0.30182743573155335 1104 46-47 0.3295197095828968 1104 48-49 0.23824110714327418 1104 50-51 0.3773869710225384 1104 52-53 0.3239054646155495 1104 54-55 0.32412741390881905 1104 56-57 0.4402748515927115 1104 58-59 0.5390911621845405 1104 60-61 0.46321794726770094 1104 62-63 0.4575456263259525 1104 64-65 0.36087621500009703 1104 66-67 0.32334338701721066 1104 68-69 0.19143877465921122 1104 70-71 0.13698673690974061 1104 72-73 0.12899899687251803 1104 74-75 0.07773724879293908 1104 76-77 0.16705697169287603 1104 78-79 0.3760772610356504 1104 80-81 0.36896964359740636 1104 82-83 0.3921479447473146 1104 84-85 0.2736917121647835 1104 86-87 0.342790322170476 1104 88-89 0.4731400488050923 1104 90-91 0.49869280658559845 1104 92-93 0.4151150826127967 1104 94-95 0.42744422818174854 1104 96-97 0.3784806850971165 1104 98-99 0.33752912434849947 1104 100-101 0.03027374190859078 1105 1 0.03389105952006588 1105 2 0.04217350671204656 1105 3 0.0771428235542686 1105 4 0.07960375670642605 1105 5 0.1070852362998167 1105 6 0.06678359994965177 1105 7 0.08463880797489054 1105 8 0.10227632021318556 1105 9 0.10877119583052064 1105 10-11 0.0830060162733659 1105 12-13 0.09826918592336398 1105 14-15 0.11080567613925041 1105 16-17 0.15246077826219562 1105 18-19 0.11272940127577868 1105 20-21 0.2103909636510295 1105 22-23 0.15247078185623764 1105 24-25 0.13036798732625243 1105 26-27 0.16314352516806707 1105 28-29 0.14863329671884173 1105 30-31 0.1284236736453508 1105 32-33 0.10852712721902691 1105 34-35 0.23822604818639093 1105 36-37 0.14055769674698837 1105 38-39 0.2826336366337472 1105 40-41 0.18317244276445166 1105 42-43 0.21513110653899048 1105 44-45 0.18249881306508797 1105 46-47 0.24768886258388534 1105 48-49 0.23642542584475024 1105 50-51 0.2089462035450964 1105 52-53 0.1823064668394423 1105 54-55 0.1707832696486662 1105 56-57 0.29637506682762194 1105 58-59 0.3911495751691021 1105 60-61 0.31049415705869166 1105 62-63 0.271249392623929 1105 64-65 0.04638934951610452 1105 66-67 0.053243468658031645 1105 68-69 0.05741144957222133 1105 70-71 0.1809455838515177 1105 72-73 0.08616036403896032 1105 74-75 -0.025894963297954376 1105 76-77 -0.03045235709074845 1105 78-79 0.09203200715770876 1105 80-81 -0.06359775926744149 1105 82-83 0.009750306519020313 1105 84-85 0.04687059857672793 1105 86-87 0.1173858513291286 1105 88-89 0.3473941708621524 1105 90-91 0.4021976840215231 1105 92-93 0.4936413985905226 1105 94-95 0.33969889212645654 1105 96-97 0.18222860719048484 1105 98-99 0.040086682026895915 1105 100-101 -0.15587919559638763 1106 1 0.06134823293049152 1106 2 0.11351213410235772 1106 3 0.10218356312081056 1106 4 0.04106162982306927 1106 5 0.11021269388348998 1106 6 0.10687424769567855 1106 7 0.011809803525551388 1106 8 0.07451343205909211 1106 9 0.133872201237331 1106 10-11 0.10700080398552814 1106 12-13 0.11590097606922001 1106 14-15 0.07297740237784467 1106 16-17 0.04320107337524348 1106 18-19 -0.007375113272267697 1106 20-21 0.14795229935142373 1106 22-23 0.1464677524367417 1106 24-25 0.16383915401208782 1106 26-27 0.20051747088722038 1106 28-29 0.10701336790893379 1106 30-31 0.21633533637281488 1106 32-33 0.20206183334029504 1106 34-35 0.09180530858871805 1106 36-37 0.16287296320193434 1106 38-39 0.22652847359130135 1106 40-41 0.13487934536763646 1106 42-43 0.08510465605975526 1106 44-45 0.08229539463384583 1106 46-47 0.2387573518819579 1106 48-49 0.24759823163412165 1106 50-51 0.25309758058417486 1106 52-53 0.44147568101107737 1106 54-55 0.298839692056184 1106 56-57 0.38934842352599475 1106 58-59 0.3699129399326253 1106 60-61 0.36041018806928804 1106 62-63 0.385217684586749 1106 64-65 0.4833430753950978 1106 66-67 0.38407633549698517 1106 68-69 0.2741503291453711 1106 70-71 0.34002207916937977 1106 72-73 0.23319203798208576 1106 74-75 0.3317588903700788 1106 76-77 0.3754321353626544 1106 78-79 0.38953715698693614 1106 80-81 0.25495417425452516 1106 82-83 0.36838722902266596 1106 84-85 0.44970494631430213 1106 86-87 0.6085040670188739 1106 88-89 0.7954296671591301 1106 90-91 0.8252611947387258 1106 92-93 0.7893276093560111 1106 94-95 0.6338735762011822 1106 96-97 0.6571587249875677 1106 98-99 0.7307823719300046 1106 100-101 0.3413198575225742 1107 1 0.02556649229580188 1107 2 0.008976853688352548 1107 3 -0.026937861119570528 1107 4 0.0036507633742388634 1107 5 -4.854664240596662E-4 1107 6 0.10328276885709187 1107 7 0.04182929333267538 1107 8 0.03629212245589031 1107 9 0.05556623814518247 1107 10-11 0.061936382534561574 1107 12-13 0.02123189554264826 1107 14-15 0.07669970024893757 1107 16-17 0.032270288867621844 1107 18-19 0.10790758888568774 1107 20-21 0.13104024175377305 1107 22-23 0.10451658082634907 1107 24-25 -3.7391851703461043E-4 1107 26-27 0.05589855003820787 1107 28-29 -0.028003557520975164 1107 30-31 0.08485788634575187 1107 32-33 0.16618216415778875 1107 34-35 0.10429646631622091 1107 36-37 0.16373662545677092 1107 38-39 0.0923535985987769 1107 40-41 0.14354183456003966 1107 42-43 0.09045587713418968 1107 44-45 0.134377781043149 1107 46-47 0.12954648983654238 1107 48-49 0.13273999907826806 1107 50-51 0.0670596115925477 1107 52-53 0.24982248908747096 1107 54-55 0.11945904081918712 1107 56-57 0.1966300391498237 1107 58-59 0.0588154877903051 1107 60-61 0.15157918073032306 1107 62-63 0.07741259022212432 1107 64-65 0.028725865931875205 1107 66-67 0.2590847944974044 1107 68-69 0.12635649723911513 1107 70-71 0.29196948627132 1107 72-73 0.21251824094650118 1107 74-75 0.23869745891405003 1107 76-77 0.2653542342159163 1107 78-79 0.509657934628752 1107 80-81 0.41607635166674584 1107 82-83 0.3122802494456991 1107 84-85 0.3541481603859751 1107 86-87 0.34231056858296327 1107 88-89 0.47183241256232833 1107 90-91 0.5875476818012864 1107 92-93 0.5628406251406091 1107 94-95 0.5896132494332065 1107 96-97 0.4919520491511875 1107 98-99 0.4161770516678587 1107 100-101 -0.02360344686487892 1108 1 -0.07846535141045763 1108 2 0.020306670357364 1108 3 -0.010667609143403922 1108 4 0.02211422100388205 1108 5 8.782551170654074E-4 1108 6 0.022268397986650257 1108 7 0.05096952542010058 1108 8 0.07005564344136417 1108 9 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100-101 -0.26525469805202206 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 10.0 4 27.0 5 54.0 6 134.0 7 342.0 8 686.0 9 1314.0 10 2190.0 11 3475.0 12 4907.0 13 7247.0 14 8732.0 15 11494.0 16 15117.0 17 21364.0 18 20703.0 19 14749.0 20 14234.0 21 15719.0 22 16270.0 23 18015.0 24 20941.0 25 24120.0 26 29024.0 27 34653.0 28 42571.0 29 51328.0 30 63175.0 31 77589.0 32 97653.0 33 126536.0 34 175817.0 35 264856.0 36 420886.0 37 673365.0 38 518400.0 39 36219.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.60784071448777 19.16826015670666 14.638098674001501 23.58580045480407 2 15.935864012906523 20.55223231740108 38.98199523203934 24.529908437653056 3 19.436532346054012 24.066733100063658 29.270133624285265 27.226600929597065 4 13.773520457204802 16.683486737080422 35.23844743457463 34.30454537114015 5 14.83816034067347 36.5055280396455 33.09671140570151 15.559600213979524 6 33.20228969383708 34.948248289645846 16.697354473456517 15.152107543060556 7 29.646538570656293 31.881961215505328 20.115592699289607 18.355907514548772 8 26.490622869555768 34.790516020940636 19.414866213395175 19.303994896108424 9 26.626441997575085 17.017053434187886 20.124626135707622 36.2318784325294 10-11 25.83834524382515 26.067392258627287 26.27809010570532 21.816172391842244 12-13 26.40690479181458 23.315475829205948 27.1940488003173 23.083570578662176 14-15 23.254029488446886 24.666046435473223 25.37544247932038 26.704481596759504 16-17 23.23962560665833 28.044336629362483 25.753083818933476 22.962953945045715 18-19 23.047127383848846 26.306893467164684 28.31161132214622 22.334367826840253 20-21 23.521392306617418 25.56910649433505 28.23525820807674 22.674242990970797 22-23 23.33525411480086 25.355850349833943 28.272080047538463 23.03681548782674 24-25 23.64354871833959 25.630102946396878 27.99865697543528 22.72769135982825 26-27 22.9383375007675 26.07924547210572 28.130268840084156 22.85214818704263 28-29 22.80764496901108 26.13519596205392 28.398248219072126 22.658910849862878 30-31 22.836223626410174 25.803750373391154 28.11412633351148 23.24589966668719 32-33 22.70968864285321 25.974940682786645 28.11346560730805 23.201905067052092 34-35 23.601680896172105 25.82780078045389 27.74562108548908 22.824897237884922 36-37 23.326369819919908 25.634053574873185 27.715642279544973 23.323934325661938 38-39 22.987808568961448 26.035339849618488 28.10932845744329 22.867523123976774 40-41 23.12093215966368 25.81201522071949 28.097028910473455 22.970023709143373 42-43 23.080902786539195 25.952122111607316 27.559509451654247 23.407465650199242 44-45 23.133897001220966 25.994223345166596 27.477433287929365 23.394446365683073 46-47 23.19102090233128 26.275671383007797 27.25242737803298 23.280880336627945 48-49 23.414484476985514 26.347979755029222 27.045060692214832 23.19247507577043 50-51 23.152276877893417 26.366665743326323 27.064181740197785 23.416875638582475 52-53 23.622081419606538 26.2905834761277 26.832960938801204 23.254374165464558 54-55 23.105783657666635 26.166671842072947 27.02449543317445 23.703049067085967 56-57 23.14364645952809 26.655112572143985 26.896139476258295 23.305101492069628 58-59 23.386612552873252 26.030588455743676 27.049774303346958 23.53302468803611 60-61 23.399645362601653 26.125296156106803 27.287405043445638 23.187653437845906 62-63 23.126310862067537 26.366459293073603 27.196200685693235 23.311029159165628 64-65 22.97664360792625 26.129810351608295 27.073120855571194 23.82042518489426 66-67 22.93317801680651 26.55288001939457 26.80300502896658 23.71093693483234 68-69 22.887174155036398 26.24047416098247 27.19323891495023 23.6791127690309 70-71 22.952615542917755 26.346384126973483 27.056497293760977 23.644503036347782 72-73 23.07825990608049 26.155962279757055 26.97084176101197 23.79493605315048 74-75 22.91354083889572 26.605569819288927 26.65726507066547 23.823624271149885 76-77 22.992868960271387 26.345099463425992 26.92066070724793 23.74137086905469 78-79 22.92099262978507 26.43202942651778 26.888978164946515 23.75799977875064 80-81 22.70532986379449 26.806933855034536 26.87057617917387 23.617160101997104 82-83 22.997346586228538 26.44736672634304 26.737462006816408 23.817824680612013 84-85 22.74677513377249 26.340830144577325 26.703984874639897 24.208409847010284 86-87 22.883984467795933 26.272774398886572 26.94194498731407 23.901296146003425 88-89 22.659604619854036 26.329448026642105 26.736838375965423 24.27410897753844 90-91 22.574763043647295 26.342560784686718 26.979521848798882 24.103154322867102 92-93 22.811438306569425 26.07665505964185 26.94377673861893 24.168129895169795 94-95 22.526532190791823 25.799512053285984 27.08802942641913 24.585926329503064 96-97 22.736845893958378 25.81206108650734 27.018007417652363 24.433085601881917 98-99 22.364967985419206 25.991957979649698 26.769800255128594 24.873273779802496 100-101 22.42769350908459 25.207051857842828 27.517584918124072 24.84766971494851 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1973.0 1 1346.5 2 998.5 3 1249.5 4 1038.0 5 1054.5 6 1875.0 7 3676.5 8 5538.0 9 6003.5 10 4920.5 11 3812.5 12 3665.5 13 3809.0 14 3817.5 15 3780.0 16 3823.5 17 3812.0 18 3818.0 19 3879.0 20 4053.5 21 4457.0 22 5171.5 23 6149.5 24 7153.0 25 8446.0 26 10211.5 27 12456.0 28 14440.0 29 17025.0 30 21377.0 31 25827.0 32 30889.5 33 35643.5 34 39665.5 35 43718.5 36 49753.0 37 56994.5 38 62485.0 39 64591.0 40 66239.5 41 74298.5 42 82579.0 43 89215.0 44 94434.5 45 97805.0 46 97657.5 47 101328.0 48 117477.0 49 124136.5 50 120028.0 51 117087.5 52 112195.5 53 113278.0 54 113899.0 55 104913.5 56 95104.0 57 86317.0 58 77854.0 59 69964.5 60 61037.0 61 50053.5 62 40167.0 63 31456.0 64 24146.0 65 18975.0 66 14568.0 67 12519.5 68 10061.5 69 7020.0 70 5393.0 71 4282.0 72 3616.5 73 3140.0 74 2575.5 75 2139.5 76 1479.0 77 810.5 78 465.5 79 300.5 80 187.5 81 129.0 82 76.0 83 33.5 84 22.0 85 15.5 86 10.5 87 5.0 88 2.0 89 2.5 90 1.0 91 0.5 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.33180235405707154 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.351018167087522E-4 16-17 0.0018172733419056883 18-19 3.5286861007877436E-5 20-21 0.0 22-23 0.0 24-25 4.940160541102841E-4 26-27 0.0013409007182993427 28-29 0.0 30-31 0.0032287477822207857 32-33 0.0 34-35 0.01088599662093019 36-37 0.02877643515192405 38-39 0.02313053739066366 40-41 0.014467613013229751 42-43 0.010991857203953822 44-45 0.003387538656756234 46-47 0.0016055521758584234 48-49 9.527452472126908E-4 50-51 8.82171525196936E-5 52-53 1.4114744403150974E-4 54-55 0.0 56-57 0.0 58-59 0.004746082805559515 60-61 0.011203578370001088 62-63 0.004993090832614657 64-65 0.0023465762570238496 66-67 0.004693152514047699 68-69 0.003899198141370457 70-71 5.2930291511816154E-5 72-73 0.0 74-75 0.0 76-77 0.21410302916529633 78-79 3.3522517957483566E-4 80-81 0.003881554710866518 82-83 6.8809378965361E-4 84-85 0.0 86-87 0.10668982425731743 88-89 0.3993061191651411 90-91 0.3874673772969982 92-93 0.0 94-95 0.0 96-97 1.7643430503938718E-5 98-99 0.0017996299114017495 100-101 8.645280946929972E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2833916.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.97328978746254 #Duplication Level Percentage of deduplicated Percentage of total 1 65.67415030485209 23.62515240461816 2 15.836871984371934 11.39408770441517 3 6.615322442958307 7.139247338341316 4 3.4137537257736064 4.912158081611356 5 2.101500995512729 3.7798952150110208 6 1.3091115409091318 2.8255829295141495 7 0.9126214180131045 2.2980996315501274 8 0.6485327406491701 1.8663884972823908 9 0.4784548087131327 1.5490434135638231 >10 2.608514640436366 17.193952739441112 >50 0.22550066182064868 5.670321513411363 >100 0.15908565831701846 11.317788617548263 >500 0.011379721952914796 2.7530218789996295 >1k 0.0049050525659080375 2.703086280700664 >5k 1.962021026363215E-4 0.5604079865466415 >10k+ 9.810105131816075E-5 0.41176576744497556 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11668 0.411727094239914 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9186 0.32414510521836215 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6694 0.23621024758673156 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4037 0.14245305788880122 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 7.057372201575487E-5 0.0 0.0 0.0 0.0 3 7.057372201575487E-5 0.0 0.0 0.0 0.0 4 7.057372201575487E-5 0.0 0.0 0.0 0.0 5 1.4114744403150974E-4 0.0 0.0 0.0 0.0 6 1.764343050393872E-4 0.0 0.0 0.0 0.0 7 2.470080270551421E-4 0.0 0.0 0.0 0.0 8 3.1758174907089694E-4 0.0 0.0 0.0 0.0 9 3.1758174907089694E-4 0.0 0.0 0.0 0.0 10-11 3.528686100787744E-4 0.0 0.0 0.0 0.0 12-13 3.8815547108665184E-4 0.0 0.0 0.0 0.0 14-15 5.64589776126039E-4 0.0 0.0 0.0 0.0 16-17 5.998766371339164E-4 0.0 0.0 5.2930291511816154E-5 0.0 18-19 7.233806506614875E-4 0.0 0.0 7.057372201575487E-5 0.0 20-21 8.115978031811811E-4 0.0 0.0 8.82171525196936E-5 0.0 22-23 8.115978031811811E-4 0.0 0.0 2.2936459655120334E-4 1.7643430503938718E-5 24-25 0.0010233189692284458 0.0 0.0 5.293029151181615E-4 3.5286861007877436E-5 26-27 0.0010586058302363233 0.0 0.0 0.0010409623997323844 3.5286861007877436E-5 28-29 0.0012173967047717717 0.0 0.0 0.003087600338189276 3.5286861007877436E-5 30-31 0.0012703269962835878 0.0 0.0 0.009333374736583583 3.5286861007877436E-5 32-33 0.0013056138572914652 0.0 0.0 0.020201727927009833 3.5286861007877436E-5 34-35 0.0015702653148505462 0.0 0.0 0.03556915589594046 3.5286861007877436E-5 36-37 0.0017290561893859946 0.0 0.0 0.060358175753974355 3.5286861007877436E-5 38-39 0.0018702036334175042 3.5286861007877436E-5 0.0 0.09762110097829294 3.5286861007877436E-5 40-41 0.001887847063921443 3.5286861007877436E-5 0.0 0.13952424842514738 3.5286861007877436E-5 42-43 0.001993707646945075 3.5286861007877436E-5 0.0 0.18326231264441148 3.5286861007877436E-5 44-45 0.002187785382488401 3.5286861007877436E-5 0.0 0.23758643516603878 3.5286861007877436E-5 46-47 0.0024877237010553594 3.5286861007877436E-5 0.0 0.3042609590404232 3.5286861007877436E-5 48-49 0.0025406539925671755 3.5286861007877436E-5 0.0 0.3799689193328243 3.5286861007877436E-5 50-51 0.0025406539925671755 3.5286861007877436E-5 0.0 0.46116398651195023 3.5286861007877436E-5 52-53 0.0025406539925671755 3.5286861007877436E-5 0.0 0.5446879865175962 3.5286861007877436E-5 54-55 0.002575940853575053 7.057372201575487E-5 0.0 0.6406682484590227 3.5286861007877436E-5 56-57 0.0026112277145829304 7.057372201575487E-5 0.0 0.7571678200765302 3.5286861007877436E-5 58-59 0.0026112277145829304 7.057372201575487E-5 0.0 0.8872528331820704 3.5286861007877436E-5 60-61 0.0026112277145829304 7.057372201575487E-5 0.0 1.0287884326846668 3.5286861007877436E-5 62-63 0.0026112277145829304 7.057372201575487E-5 0.0 1.1754229836029015 3.5286861007877436E-5 64-65 0.0026112277145829304 7.057372201575487E-5 0.0 1.3359252708972318 5.2930291511816154E-5 66-67 0.0026818014365986853 7.057372201575487E-5 0.0 1.5243041783877858 7.057372201575487E-5 68-69 0.002717088297606563 7.057372201575487E-5 0.0 1.741000791837161 7.057372201575487E-5 70-71 0.002840592311134134 7.057372201575487E-5 0.0 1.9809867335517355 7.057372201575487E-5 72-73 0.002911166033149889 7.057372201575487E-5 0.0 2.2255952540583417 7.057372201575487E-5 74-75 0.002964096324661705 7.057372201575487E-5 0.0 2.4860652185879895 7.057372201575487E-5 76-77 0.003017026616173521 7.057372201575487E-5 0.0 2.775523339435608 7.057372201575487E-5 78-79 0.00303467004667746 7.057372201575487E-5 0.0 3.102526680395608 7.057372201575487E-5 80-81 0.0033169649347404794 7.057372201575487E-5 0.0 3.463899423977281 8.82171525196936E-5 82-83 0.0033522517957483566 7.057372201575487E-5 0.0 3.8318531671369227 1.0586058302363232E-4 84-85 0.0033698952262522955 7.057372201575487E-5 0.0 4.2132688477710705 1.0586058302363232E-4 86-87 0.0033875386567562343 7.057372201575487E-5 0.0 4.6192441836667 1.0586058302363232E-4 88-89 0.0033875386567562343 7.057372201575487E-5 0.0 5.064846664474176 1.0586058302363232E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13785 0.0 28.560991 1 GGTATCA 9765 0.0 27.35049 1 GTACATG 10775 0.0 25.140247 1 TACATGG 11050 0.0 23.875042 2 ACATGGG 11035 0.0 22.961514 3 ATCAACG 17165 0.0 22.833256 3 TCAACGC 17285 0.0 22.619837 4 CAACGCA 17340 0.0 22.548088 5 TATCAAC 17515 0.0 22.458256 2 AACGCAG 17745 0.0 22.086946 6 GAGTACT 9060 0.0 21.68375 12-13 CATGGGG 6560 0.0 21.193144 4 AGTACTT 9625 0.0 21.15041 12-13 ATGGGGA 3950 0.0 20.78168 5 AGAGTAC 15540 0.0 20.291128 10-11 GCACCGT 660 0.0 20.130089 6 ACGCAGA 19575 0.0 19.900911 7 CGCAGAG 19675 0.0 19.727413 8 GTACTTT 10130 0.0 19.58074 14-15 TACTTTT 11080 0.0 18.437227 14-15 >>END_MODULE