FastQCFastQC Report
Fri 27 May 2016
SRR939062_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939062_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2794642
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117120.41908766847417306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83200.29771255137509567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59870.21423137561090116No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36740.1314658550182814No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87450.029.7044561
GTATCAA127050.029.6409841
GTACATG106700.025.5916021
TACATGG110250.024.3617152
TATCAAC156600.023.8967462
ATCAACG156050.023.8296173
ACATGGG110350.023.5244463
TCAACGC158650.023.4390894
CAACGCA158850.023.3785025
AACGCAG162000.022.9988676
GAGTACT86950.021.53452312-13
CATGGGG64400.020.6695794
ACGCAGA183100.020.3226787
CGCAGAG185200.020.0922378
GTACTTT94650.019.80782714-15
ATGGGGA39400.019.3560835
AGAGTAC146450.019.34752310-11
GTACTAG3455.2750693E-1119.2709471
AGTACTT94050.019.17893612-13
GCAGAGT197050.018.5956429
Produced by FastQC (version 0.11.5)