FastQCFastQC Report
Fri 27 May 2016
SRR939062_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939062_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2794642
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114880.4110723305525359No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89170.31907485824660187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64700.23151444800443133No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40260.144061386037997No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97450.028.4133911
GTATCAA137200.027.8926771
GTACATG104750.025.5233481
TACATGG107450.024.380322
ACATGGG109950.022.962713
ATCAACG169000.022.4932673
TATCAAC171300.022.3020732
TCAACGC172000.022.1009434
GAGTACT94900.021.98053412-13
CAACGCA174150.021.828095
AACGCAG178000.021.409296
GGACCGA4350.020.7286516
CATGGGG65900.020.5962124
GTACTTT102300.020.50653314-15
AGTACTT101350.020.11337712-13
TATAACG1200.00478979219.7740442
AGAGTAC157500.019.33080910-11
ACGCAGA198350.019.117077
CGCAGAG200300.018.883578
GTAAGGT5700.018.3170094
Produced by FastQC (version 0.11.5)