##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939062_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2794642 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.451967729677 34.0 31.0 34.0 31.0 34.0 2 32.67872986951459 34.0 31.0 34.0 31.0 34.0 3 32.78263727518588 34.0 31.0 34.0 31.0 34.0 4 36.25071297146468 37.0 35.0 37.0 35.0 37.0 5 36.20401325107116 37.0 37.0 37.0 35.0 37.0 6 36.24105413144152 37.0 37.0 37.0 35.0 37.0 7 36.19205930491276 37.0 37.0 37.0 35.0 37.0 8 36.16549847887493 37.0 37.0 37.0 35.0 37.0 9 37.903311765871976 39.0 38.0 39.0 35.0 39.0 10-11 37.8100135902917 39.0 38.0 39.0 35.0 39.0 12-13 37.71476614893786 39.0 38.0 39.0 35.0 39.0 14-15 39.1162812625016 40.5 38.5 41.0 36.0 41.0 16-17 39.22025647650039 41.0 39.0 41.0 36.0 41.0 18-19 39.233204109864516 41.0 39.0 41.0 36.0 41.0 20-21 39.191426844654885 41.0 39.0 41.0 36.0 41.0 22-23 39.114231805004 40.5 39.0 41.0 35.5 41.0 24-25 39.025637809780285 40.0 38.5 41.0 35.0 41.0 26-27 38.87703559167865 40.0 38.0 41.0 35.0 41.0 28-29 38.711130083924886 40.0 38.0 41.0 35.0 41.0 30-31 38.50634428309601 40.0 38.0 41.0 34.0 41.0 32-33 38.424917932243204 40.0 38.0 41.0 34.0 41.0 34-35 38.30959260613703 40.0 38.0 41.0 34.0 41.0 36-37 38.15317310768249 40.0 38.0 41.0 33.5 41.0 38-39 38.01695369210081 40.0 38.0 41.0 33.0 41.0 40-41 37.81819657043729 40.0 37.0 41.0 33.0 41.0 42-43 37.616147971725894 40.0 37.0 41.0 33.0 41.0 44-45 37.35231596748349 40.0 37.0 41.0 32.5 41.0 46-47 37.029292124000136 40.0 36.0 41.0 31.5 41.0 48-49 36.68040503935745 39.5 36.0 41.0 31.0 41.0 50-51 36.39840237139498 39.0 35.0 41.0 30.0 41.0 52-53 36.10115857415727 39.0 35.0 40.0 30.0 41.0 54-55 35.81010966699849 38.5 35.0 40.0 29.0 41.0 56-57 35.52317076749007 38.0 35.0 40.0 28.5 41.0 58-59 35.146914345379486 38.0 34.0 40.0 28.0 41.0 60-61 34.72504456742581 37.5 34.0 40.0 27.0 41.0 62-63 34.286143627699005 37.0 34.0 39.5 26.0 41.0 64-65 33.7848973142177 36.0 33.0 39.0 26.0 40.5 66-67 33.327987627753394 35.5 33.0 39.0 25.0 40.0 68-69 32.8017356069221 35.0 32.0 38.0 23.5 40.0 70-71 32.254949650080405 35.0 31.5 37.5 22.0 39.5 72-73 31.64047237535255 35.0 31.0 37.0 20.5 39.0 74-75 31.062010268220405 34.0 30.5 36.0 18.5 39.0 76-77 29.097571352609744 32.5 28.0 34.5 14.0 36.0 78-79 30.115956176139914 34.0 29.5 35.0 16.5 37.0 80-81 30.123545341406878 34.0 30.0 35.0 13.5 37.0 82-83 29.72318457963489 34.0 29.5 35.0 7.5 36.0 84-85 29.219802035466437 34.0 29.0 35.0 4.5 36.0 86-87 28.477026574423483 33.5 28.5 35.0 2.0 35.5 88-89 27.76238262360617 33.0 26.5 35.0 2.0 35.0 90-91 27.159887026674614 32.5 25.5 34.5 2.0 35.0 92-93 26.626079476369426 32.0 25.0 34.0 2.0 35.0 94-95 25.990041658287538 32.0 24.5 34.0 2.0 35.0 96-97 25.06006744334337 31.5 21.0 34.0 2.0 35.0 98-99 23.6746712101228 31.0 2.0 34.0 2.0 35.0 100-101 17.79734381004794 23.5 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15181608110811595 1101 2 0.14219757068841687 1101 3 0.20042292857880284 1101 4 0.1285596151244519 1101 5 0.14140413456773615 1101 6 0.09859050469295028 1101 7 0.09625376963766286 1101 8 0.1540749904979961 1101 9 0.13586822903649676 1101 10-11 0.1690899271779429 1101 12-13 0.17487318364050708 1101 14-15 0.25077184289197163 1101 16-17 0.2406613862213547 1101 18-19 0.19410745759826398 1101 20-21 0.14705710190806087 1101 22-23 0.3021302163294095 1101 24-25 0.253928281899384 1101 26-27 0.21862230441574582 1101 28-29 0.2401839005292814 1101 30-31 0.2667724908564324 1101 32-33 0.32956235926477717 1101 34-35 0.31827574443761364 1101 36-37 0.3696320420100889 1101 38-39 0.28830057716358937 1101 40-41 0.3875668908437433 1101 42-43 0.4477176593905625 1101 44-45 0.3800277710783959 1101 46-47 0.4932029806162106 1101 48-49 0.5026889839889108 1101 50-51 0.4351004624075969 1101 52-53 0.4972946333854651 1101 54-55 0.540493162956011 1101 56-57 0.6031288121805929 1101 58-59 0.4604801126346558 1101 60-61 0.4318334525957752 1101 62-63 0.34491781084495443 1101 64-65 0.3596733631745508 1101 66-67 0.2820109342751138 1101 68-69 0.4114049494353722 1101 70-71 0.2832918761341432 1101 72-73 0.43179067157570117 1101 74-75 0.2703270125537429 1101 76-77 0.5740896219498062 1101 78-79 0.6262797761743712 1101 80-81 0.6873792681801909 1101 82-83 0.5381038705517582 1101 84-85 0.5658556195929165 1101 86-87 0.6096735414463481 1101 88-89 0.5343690783639197 1101 90-91 0.6641580856947904 1101 92-93 0.40055123312126284 1101 94-95 0.27191791001927257 1101 96-97 0.10854006235812363 1101 98-99 -0.12989568760518466 1101 100-101 -0.9079896414364867 1102 1 0.09554415679919259 1102 2 0.09581337551439617 1102 3 0.07391704614165917 1102 4 0.10608141546614291 1102 5 0.1071073840941068 1102 6 0.1298190021282366 1102 7 0.14208432968330698 1102 8 0.19938309892664563 1102 9 0.20855546046211515 1102 10-11 0.21995585750477886 1102 12-13 0.22523174290093806 1102 14-15 0.3061437906141009 1102 16-17 0.260473395656156 1102 18-19 0.26779077217036473 1102 20-21 0.27691418940899126 1102 22-23 0.10393114079272436 1102 24-25 0.15492293367157828 1102 26-27 0.19835787846329822 1102 28-29 0.16689523629799652 1102 30-31 0.22150042238766332 1102 32-33 0.17599123376495385 1102 34-35 0.17216616864583045 1102 36-37 0.37349470778112703 1102 38-39 0.23444077414114162 1102 40-41 0.3091188890933765 1102 42-43 0.383996965501197 1102 44-45 0.361621320078946 1102 46-47 0.41994601110142327 1102 48-49 0.2949344626353607 1102 50-51 0.2222722303900042 1102 52-53 0.30570543985241727 1102 54-55 0.20809763538723303 1102 56-57 0.1799251102377042 1102 58-59 -0.007749768121641409 1102 60-61 -0.05245516971668707 1102 62-63 -0.013628413854227972 1102 64-65 -0.03806951292671101 1102 66-67 0.016656633353200334 1102 68-69 -0.03358784768469292 1102 70-71 0.02312656262738244 1102 72-73 0.08395981707591815 1102 74-75 0.01262494084393495 1102 76-77 0.02278073239975953 1102 78-79 0.291181251614546 1102 80-81 0.2677611588415978 1102 82-83 0.11251386318017964 1102 84-85 -0.004686850815978971 1102 86-87 0.025666695387585037 1102 88-89 0.00706152408160321 1102 90-91 0.29906664643771563 1102 92-93 0.18072434761791456 1102 94-95 0.04830427270293214 1102 96-97 0.03629783497613914 1102 98-99 0.12752752172001536 1102 100-101 -0.24363413304812198 1103 1 0.05695787835024646 1103 2 0.05971117523414904 1103 3 0.11855729491279021 1103 4 0.051039977006198 1103 5 0.14352649342938406 1103 6 0.11764863750116916 1103 7 0.08555544992130848 1103 8 0.08353987560320064 1103 9 0.24848088196849716 1103 10-11 0.1398658877011414 1103 12-13 0.06311443040202391 1103 14-15 0.08748323092859778 1103 16-17 0.19686038471461842 1103 18-19 0.20405464726295008 1103 20-21 0.2352139781087672 1103 22-23 0.22739207171927234 1103 24-25 0.1912687195986038 1103 26-27 0.18972024885714944 1103 28-29 0.25365028739731343 1103 30-31 0.2402795546117389 1103 32-33 0.2022090587845824 1103 34-35 0.22939525392806104 1103 36-37 0.21518319394174057 1103 38-39 0.2846815110744174 1103 40-41 0.1965945408501213 1103 42-43 0.20289202797884798 1103 44-45 0.18308815155813107 1103 46-47 0.2029028601105196 1103 48-49 0.09407365573107285 1103 50-51 0.12258088230446162 1103 52-53 0.18217564437352962 1103 54-55 0.03253307404369821 1103 56-57 0.168130610649321 1103 58-59 0.076742331033401 1103 60-61 0.1476707516769764 1103 62-63 0.16597058637119488 1103 64-65 0.16364615574065056 1103 66-67 0.03012287362113142 1103 68-69 -0.03222938291832378 1103 70-71 -0.10463658624968275 1103 72-73 0.027925952891020955 1103 74-75 0.09459307984133503 1103 76-77 0.07896363797192407 1103 78-79 0.06062058351278665 1103 80-81 0.2656552577109501 1103 82-83 0.19106156426838794 1103 84-85 -0.052554815835428315 1103 86-87 0.09189953188877098 1103 88-89 0.10349807249776077 1103 90-91 0.3058003445715194 1103 92-93 0.3190981698400286 1103 94-95 0.1773424066552245 1103 96-97 0.03621518099904364 1103 98-99 0.011965454032932143 1103 100-101 -0.4373108148005862 1104 1 0.06928510298536139 1104 2 0.11474873156468846 1104 3 0.14215197339589736 1104 4 0.03728245008996822 1104 5 0.0852799885572395 1104 6 0.0568464479104378 1104 7 0.09896082928422345 1104 8 0.11712004503656459 1104 9 0.10453050592974478 1104 10-11 0.09530709831533812 1104 12-13 0.1546607496122121 1104 14-15 0.0825031640332341 1104 16-17 0.06412238994294484 1104 18-19 0.20480838883069907 1104 20-21 0.26522457264699284 1104 22-23 0.13894598222909593 1104 24-25 0.12863748639276906 1104 26-27 0.22017427672308543 1104 28-29 0.17536924924350217 1104 30-31 0.1898795850403232 1104 32-33 0.1399573113196979 1104 34-35 0.2066382333626109 1104 36-37 0.16314245766491808 1104 38-39 0.18937393269107616 1104 40-41 0.19400525789512812 1104 42-43 0.2908632254152437 1104 44-45 0.33892049635844756 1104 46-47 0.33175542486834786 1104 48-49 0.35326915229953926 1104 50-51 0.24012716506373266 1104 52-53 0.20850881684738454 1104 54-55 0.14589899132705852 1104 56-57 0.2516368877349251 1104 58-59 0.32972025207912736 1104 60-61 0.3234725727964829 1104 62-63 0.1731644917778894 1104 64-65 0.2240581620387445 1104 66-67 0.32325900749425074 1104 68-69 0.36699896701998114 1104 70-71 0.33099386801590214 1104 72-73 0.18935454356386217 1104 74-75 0.11830535215187155 1104 76-77 0.05787939752582716 1104 78-79 0.3932479576759036 1104 80-81 0.49556608767455046 1104 82-83 0.3799969676968331 1104 84-85 0.08026931403760429 1104 86-87 0.042225173286237094 1104 88-89 0.2641448702450937 1104 90-91 0.30112313725058826 1104 92-93 0.1405361426619045 1104 94-95 0.21237118306926206 1104 96-97 -0.11655283031025121 1104 98-99 0.037278000834721325 1104 100-101 -0.46144155948489285 1105 1 0.0344583125649649 1105 2 0.060795037478655445 1105 3 0.07430057315212935 1105 4 0.06162687174398229 1105 5 0.052881308773244484 1105 6 0.04291370319415222 1105 7 0.052067097390363415 1105 8 0.040469202582066544 1105 9 0.08296181429565053 1105 10-11 0.10269531647707453 1105 12-13 0.15073942777336669 1105 14-15 0.11252406268927473 1105 16-17 0.13764634840258338 1105 18-19 0.1257720813538299 1105 20-21 0.19992614632393924 1105 22-23 0.17722115078684197 1105 24-25 0.14219848429706872 1105 26-27 0.23597459197790727 1105 28-29 0.17594431190182291 1105 30-31 0.17776385083730162 1105 32-33 0.17457018211947428 1105 34-35 0.1821730208883281 1105 36-37 0.2500171058386016 1105 38-39 0.14569790842411834 1105 40-41 0.15312540280722686 1105 42-43 0.17068396630556748 1105 44-45 0.09066685346252967 1105 46-47 0.05473197500568716 1105 48-49 0.012118762656932347 1105 50-51 -0.039001902386573306 1105 52-53 -0.1372210970299932 1105 54-55 -0.14630614345075799 1105 56-57 -0.023307453975846215 1105 58-59 -0.07974779652057862 1105 60-61 -0.1323637829967197 1105 62-63 -0.18486633815800957 1105 64-65 0.07095272379319795 1105 66-67 0.011086043645590848 1105 68-69 -0.1430112264343535 1105 70-71 -0.23006427801097828 1105 72-73 -0.20828576319564363 1105 74-75 -0.16080196987722317 1105 76-77 -0.5069068203952583 1105 78-79 -0.5334360100474385 1105 80-81 -0.1576584509344805 1105 82-83 -0.17373604494148154 1105 84-85 -0.3596105129272402 1105 86-87 -0.1914714853108066 1105 88-89 -0.058678048107228875 1105 90-91 0.002827901434173441 1105 92-93 0.09446335804776851 1105 94-95 -0.15881992967698366 1105 96-97 -0.32409120713672834 1105 98-99 -0.3594914901702815 1105 100-101 -1.2767301120036194 1106 1 0.06270556018697704 1106 2 0.006362904370682543 1106 3 0.06795888755164015 1106 4 0.045412520335119666 1106 5 0.02080784252420642 1106 6 0.03327063426504395 1106 7 0.07897032705808016 1106 8 0.07759186954140063 1106 9 0.0659395670114904 1106 10-11 0.039907698491241206 1106 12-13 0.0722461060574986 1106 14-15 0.034351671669455186 1106 16-17 0.007131289535344365 1106 18-19 -0.015948523005747006 1106 20-21 0.034416127152745446 1106 22-23 0.1677820607152185 1106 24-25 0.05939212728521426 1106 26-27 0.06209572200443603 1106 28-29 0.12625166133356913 1106 30-31 0.10472575691316166 1106 32-33 -0.0449422556899961 1106 34-35 -0.15232163801402265 1106 36-37 -0.009743694607998066 1106 38-39 0.11113288535526777 1106 40-41 -0.054320204192123356 1106 42-43 -0.1032684014011096 1106 44-45 0.0106769937438429 1106 46-47 -0.058600965270464656 1106 48-49 -0.01473233990559919 1106 50-51 -0.14271508984618464 1106 52-53 -0.09532233974908877 1106 54-55 -0.29055023433844696 1106 56-57 -0.23168889844912144 1106 58-59 -0.26107935743333144 1106 60-61 -0.16954763618777946 1106 62-63 -0.24675398390041892 1106 64-65 -0.24360732715176425 1106 66-67 -0.2078613868371164 1106 68-69 -0.4763378520537671 1106 70-71 -0.39453353720164586 1106 72-73 -0.46951325108054576 1106 74-75 -0.3405354117616639 1106 76-77 -0.40233836228384234 1106 78-79 -0.3397347316264714 1106 80-81 -0.10702981438203452 1106 82-83 -0.13299886074671718 1106 84-85 -0.37048406370832154 1106 86-87 -0.26236137816212945 1106 88-89 -0.36930822025587773 1106 90-91 -0.13411846898222635 1106 92-93 -0.24772925650674438 1106 94-95 -0.41626827669083255 1106 96-97 -0.8217467497405373 1106 98-99 -0.9274815714662914 1106 100-101 -1.4631514911858332 1107 1 -0.08387671012756925 1107 2 -0.10662163914879841 1107 3 0.002618313938569372 1107 4 -0.03842910619553663 1107 5 -0.02613257181900508 1107 6 -0.019895770764975396 1107 7 -0.0013232535622691444 1107 8 0.018167926796692768 1107 9 0.06345914641855899 1107 10-11 0.056052742630789965 1107 12-13 0.05166259404056461 1107 14-15 0.03776706374856076 1107 16-17 -0.0010560878735432766 1107 18-19 -0.015294277436581183 1107 20-21 0.04182236036230336 1107 22-23 -0.02748018674336805 1107 24-25 -0.03777456593117279 1107 26-27 -0.09769757675316271 1107 28-29 -0.052911441489989386 1107 30-31 0.08786628627513693 1107 32-33 -0.02347429006261592 1107 34-35 -0.11289453204031474 1107 36-37 0.02867674854816471 1107 38-39 -0.004332291912739095 1107 40-41 0.13205743391031888 1107 42-43 0.132681767368382 1107 44-45 0.07323318688725777 1107 46-47 0.04771088448951133 1107 48-49 -0.03593258210719341 1107 50-51 0.011740011272699746 1107 52-53 -0.02846750879781723 1107 54-55 -0.20481443614961847 1107 56-57 -0.11986896604218344 1107 58-59 -0.17245871143830271 1107 60-61 -0.22929045970077055 1107 62-63 -0.15598906368384746 1107 64-65 -0.1570098830010096 1107 66-67 -0.1589020337614997 1107 68-69 -0.35921723739262035 1107 70-71 -0.40125213046567154 1107 72-73 -0.567166649569387 1107 74-75 -0.65907811528227 1107 76-77 -0.6055405135069378 1107 78-79 -0.36510472355355716 1107 80-81 -0.29338450273221284 1107 82-83 -0.5043027605135677 1107 84-85 -0.46075410768800396 1107 86-87 -0.5411975287255757 1107 88-89 -0.6304233963231063 1107 90-91 -0.36715657708138494 1107 92-93 -0.12016607825452397 1107 94-95 -0.2906204435524451 1107 96-97 -0.35286405594267123 1107 98-99 -0.36014976934800913 1107 100-101 -1.168581686778829 1108 1 -0.12828301270386788 1108 2 -0.023564975038141256 1108 3 -0.008608155147051377 1108 4 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-0.9884469410179761 2316 50-51 -0.8654556689978676 2316 52-53 -0.960329619318415 2316 54-55 -0.9383980991324705 2316 56-57 -0.8115201113290951 2316 58-59 -0.6939780126074666 2316 60-61 -0.6683218523778223 2316 62-63 -0.6901641866911135 2316 64-65 -0.5953686389683099 2316 66-67 -0.5531173057530907 2316 68-69 -0.29450780755944805 2316 70-71 -0.24128766618733977 2316 72-73 -0.23045015234335864 2316 74-75 -0.3627128118294891 2316 76-77 -0.336609843731285 2316 78-79 -0.3173846534026765 2316 80-81 -0.26302714225072066 2316 82-83 -0.35465123967987466 2316 84-85 -0.3478145335831613 2316 86-87 -0.39564940564875073 2316 88-89 -0.6740125042112126 2316 90-91 -0.8778629031616418 2316 92-93 -0.9244392566303539 2316 94-95 -0.6562305056955005 2316 96-97 -0.5917679300895209 2316 98-99 -0.22859583732615718 2316 100-101 0.7355753231574695 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 10.0 4 22.0 5 65.0 6 122.0 7 317.0 8 743.0 9 1418.0 10 2271.0 11 3586.0 12 5299.0 13 7452.0 14 9410.0 15 12340.0 16 16123.0 17 22702.0 18 22819.0 19 17097.0 20 16953.0 21 18472.0 22 20120.0 23 22027.0 24 25000.0 25 29304.0 26 34429.0 27 40536.0 28 48955.0 29 59445.0 30 72469.0 31 89034.0 32 112416.0 33 146695.0 34 205743.0 35 308206.0 36 474190.0 37 632314.0 38 308152.0 39 8382.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.676525131791756 19.164610424259674 14.555860623236383 23.60300382071219 2 15.962223426113257 20.523559010420655 38.9961934301424 24.518024133323696 3 19.43848263927902 24.077681506253754 29.256162327768635 27.227673526698588 4 13.775574832125187 16.648322039101966 35.25986512762637 34.31623800114648 5 14.84057707570415 36.46406230207661 33.0764011991518 15.618959423067427 6 33.18213925075197 34.98065226243648 16.70439362179485 15.1328148650167 7 29.627229534230143 31.911350362586692 20.151811931546153 18.309608171637013 8 26.438162741417326 34.835195348813905 19.364483894538193 19.362158015230573 9 26.613534041211718 17.067445490334716 20.077526924736695 36.24149354371687 10-11 25.83440025591829 26.060457833239465 26.2719339364398 21.83320797440245 12-13 26.411737174206927 23.316653796801166 27.23162752152154 23.039981507470365 14-15 23.325458667666684 24.632203494147316 25.363538317965688 26.67879952022031 16-17 23.208233504730494 28.069730655556118 25.806636879260815 22.915398960452578 18-19 23.01752782646221 26.29175758469242 28.342592718494892 22.34812187035048 20-21 23.470126048345367 25.61048964411184 28.214454660024437 22.704929647518355 22-23 23.35615796227209 25.372230146115314 28.24851984619139 23.023092045421205 24-25 23.605952178822285 25.627213947554257 28.025071561082722 22.741762312540736 26-27 22.93633364620388 26.080432183608615 28.101578320396857 22.881655849790647 28-29 22.80118526809516 26.15234795726966 28.38572883396156 22.660737940673616 30-31 22.829399973234494 25.79949417492473 28.126590096334343 23.244515755506427 32-33 22.708400575100494 25.955793264396654 28.14258498941904 23.19322117108381 34-35 23.583589136127646 25.85644207017302 27.726583420042356 22.833385373656977 36-37 23.305817188693155 25.60284587815288 27.768303885325913 23.323033047828055 38-39 22.984300445368298 26.052130710083993 28.088355716275625 22.875213128272083 40-41 23.10308671465559 25.800200043300965 28.145537763988273 22.951175478055177 42-43 23.06532159797882 25.92648524462274 27.576407142128645 23.431786015269797 44-45 23.142680471691566 26.015534589259477 27.44634354587413 23.395441393174828 46-47 23.23389052225368 26.26949183110849 27.222630563420196 23.27398708321763 48-49 23.40902865336331 26.345825288681556 27.048369178888187 23.196776879066945 50-51 23.13924464628432 26.364901617372926 27.09342525265917 23.402428483683586 52-53 23.589372091309013 26.341495619116866 26.806277870296086 23.262854419278035 54-55 23.121673545305622 26.195233593426277 26.96492073045492 23.71817213081318 56-57 23.126118882532676 26.682729552740405 26.875688147422157 23.315463417304763 58-59 23.36126398030774 26.000585772456564 27.075122186516467 23.563028060719233 60-61 23.432680108560472 26.078380889520986 27.3401485701833 23.148790431735243 62-63 23.092289217156193 26.38105335778997 27.18928893733142 23.337368487722422 64-65 22.952510012280968 26.091492495441155 27.11517486781499 23.840822624462888 66-67 22.91861947844427 26.52758561523434 26.806106746215846 23.747688160105547 68-69 22.861467135154825 26.2167648766998 27.1977916787886 23.72397630935677 70-71 22.93169991963181 26.3217812110191 27.061698151961007 23.68482071738808 72-73 23.10535660739372 26.115348584899245 26.963149483905273 23.81614532380176 74-75 22.96310225066395 26.555279710245532 26.675223517001463 23.806394522089054 76-77 22.97233311437456 26.36067237774185 26.926144752045307 23.740849755838283 78-79 22.902475463052184 26.38996179413497 26.90946564576787 23.798097097044973 80-81 22.71669480100657 26.758485701582767 26.844829946030373 23.67998955138028 82-83 22.991907371319833 26.406799153523064 26.71263439109553 23.888659084061572 84-85 22.75930154917875 26.320741619141195 26.685367929058536 24.234588902621518 86-87 22.88662293804204 26.256123368594192 26.915240686533675 23.94201300683009 88-89 22.621747309394163 26.31266284111306 26.71451123594698 24.35107861354579 90-91 22.56719011275421 26.270452015685418 26.999391352899625 24.16296651866075 92-93 22.769267047442927 26.00896644364466 26.925559696018308 24.296206812894102 94-95 22.483921461840453 25.75886746117525 27.08427896744538 24.672932109538916 96-97 22.703238090330743 25.72652444446353 26.9998006899634 24.570436775242328 98-99 22.336353522836777 25.897853638952036 26.749949993505417 25.015842844705773 100-101 22.755196390794925 24.603489833366254 27.60787466829264 25.03343910754618 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1881.0 1 1285.5 2 955.0 3 1234.0 4 1080.5 5 1037.0 6 1754.5 7 3532.0 8 5331.0 9 5733.0 10 4702.0 11 3692.0 12 3581.5 13 3700.5 14 3757.5 15 3817.5 16 3859.0 17 3836.5 18 3861.0 19 3914.5 20 4011.0 21 4374.5 22 5104.0 23 6069.0 24 7157.0 25 8486.0 26 10231.5 27 12194.5 28 13958.5 29 16800.5 30 20969.5 31 25185.5 32 30331.5 33 34919.0 34 39083.5 35 43284.5 36 48995.0 37 56255.5 38 61851.5 39 64023.0 40 65394.5 41 73056.5 42 81323.5 43 87995.0 44 93313.5 45 96565.5 46 96698.0 47 99910.0 48 115862.0 49 122645.0 50 117860.0 51 115224.5 52 110766.5 53 111536.5 54 112026.0 55 103560.5 56 93474.0 57 84785.0 58 76812.0 59 68833.0 60 59918.0 61 49356.0 62 39876.5 63 31242.5 64 23892.0 65 18733.5 66 14743.0 67 12650.5 68 9993.5 69 6998.0 70 5507.0 71 4393.5 72 3620.5 73 3060.5 74 2505.0 75 2143.5 76 1461.0 77 786.5 78 473.0 79 301.0 80 192.0 81 117.0 82 63.5 83 35.5 84 19.5 85 8.0 86 5.0 87 4.0 88 4.5 89 4.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4169764857180276 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.303517230471738E-4 16-17 0.002164856894013616 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.367413786810619E-5 26-27 0.0031846655135076337 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011969332744587679 36-37 0.025280518935878012 38-39 0.0196805172183056 40-41 0.008838341368948151 42-43 0.008480513783160776 44-45 0.0037214068921886953 46-47 0.004938020683865769 48-49 0.0029878603413245777 50-51 6.261982751279054E-4 52-53 0.0 54-55 0.0 56-57 3.5782758578737454E-5 58-59 0.0014849844810176044 60-61 0.0025226844798009905 62-63 0.0012881793088345484 64-65 0.002647924134826572 66-67 0.002164856894013616 68-69 0.004329713788027232 70-71 1.4313103431494982E-4 72-73 0.0 74-75 0.0 76-77 0.09446648264786688 78-79 0.015654956878197637 80-81 5.18849999391693E-4 82-83 0.0 84-85 0.0 86-87 0.1029827791896064 88-89 0.4026991650451113 90-91 0.40863910296918177 92-93 0.0 94-95 1.7891379289368727E-5 96-97 3.5782758578737454E-5 98-99 1.7891379289368728E-4 100-101 3.5782758578737457E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2794642.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.364458772297866 #Duplication Level Percentage of deduplicated Percentage of total 1 66.07802953309798 24.02891780711022 2 15.662532127589712 11.391190076470535 3 6.538086644642338 7.1326194671642265 4 3.4525683002074037 5.022031104457385 5 1.9870241927746932 3.6128529668856886 6 1.3359477742256805 2.9148610654663707 7 0.8779356458189141 2.2347958217978823 8 0.6297606821809698 1.8320725090867245 9 0.48958117109965593 1.6023018880932067 >10 2.550706195818352 16.954706551465346 >50 0.22253499921793987 5.603066877036418 >100 0.1601404060194912 11.50525404770746 >500 0.010134348783177483 2.553019161418999 >1k 0.004722803316430189 2.650733309606551 >5k 1.9678347151792452E-4 0.5505410465669973 >10k+ 9.839173575896226E-5 0.41103629966606 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11488 0.4110723305525359 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8917 0.31907485824660187 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6470 0.23151444800443133 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4026 0.144061386037997 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.0734827573621236E-4 0.0 0.0 0.0 0.0 3 1.0734827573621236E-4 0.0 0.0 0.0 0.0 4 1.0734827573621236E-4 0.0 0.0 0.0 0.0 5 1.0734827573621236E-4 0.0 0.0 0.0 0.0 6 1.7891379289368728E-4 0.0 0.0 0.0 0.0 7 2.8626206862989963E-4 0.0 0.0 0.0 0.0 8 2.8626206862989963E-4 0.0 0.0 0.0 0.0 9 2.8626206862989963E-4 0.0 0.0 0.0 0.0 10-11 2.8626206862989963E-4 0.0 0.0 0.0 0.0 12-13 3.5782758578737457E-4 0.0 0.0 1.7891379289368727E-5 0.0 14-15 5.367413786810619E-4 0.0 0.0 3.5782758578737454E-5 0.0 16-17 5.546327579704306E-4 0.0 0.0 5.367413786810618E-5 0.0 18-19 6.261982751279054E-4 0.0 0.0 1.0734827573621236E-4 0.0 20-21 6.798724129960116E-4 0.0 0.0 1.4313103431494982E-4 0.0 22-23 6.798724129960116E-4 0.0 0.0 2.683706893405309E-4 0.0 24-25 8.230034473109615E-4 0.0 0.0 5.90415516549168E-4 0.0 26-27 8.230034473109615E-4 0.0 0.0 0.0010198086194940174 0.0 28-29 0.0010198086194940174 0.0 0.0 0.0032741224099544775 0.0 30-31 0.0010734827573621237 0.0 0.0 0.00914249481686742 0.0 32-33 0.0010913741366514924 0.0 0.0 0.02057508618277404 0.0 34-35 0.0012523965502558108 0.0 0.0 0.03658787064675905 0.0 36-37 0.0015386586188857105 0.0 0.0 0.06072334130811746 0.0 38-39 0.001646006894621923 0.0 0.0 0.0984562602293961 3.5782758578737454E-5 40-41 0.0016817896532006604 0.0 0.0 0.14075148086946376 3.5782758578737454E-5 42-43 0.0018249206875156103 0.0 0.0 0.1848537308177577 3.5782758578737454E-5 44-45 0.00196805172183056 0.0 0.0 0.24348378074901902 3.5782758578737454E-5 46-47 0.002182748273302985 0.0 0.0 0.3125623961852717 3.5782758578737454E-5 48-49 0.002182748273302985 0.0 0.0 0.3870978823047818 3.5782758578737454E-5 50-51 0.002182748273302985 0.0 0.0 0.47242187013578124 3.5782758578737454E-5 52-53 0.002182748273302985 0.0 0.0 0.5586762096898279 5.367413786810618E-5 54-55 0.002182748273302985 0.0 0.0 0.6567388595748579 7.156551715747491E-5 56-57 0.0022185310318817222 0.0 0.0 0.7726034318528098 7.156551715747491E-5 58-59 0.0022185310318817222 0.0 0.0 0.9026379765279418 7.156551715747491E-5 60-61 0.002236422411171091 0.0 0.0 1.046520448773045 7.156551715747491E-5 62-63 0.0022543137904604597 0.0 0.0 1.1954125072191715 7.156551715747491E-5 64-65 0.0022543137904604597 0.0 0.0 1.3557371570312047 7.156551715747491E-5 66-67 0.0022543137904604597 0.0 0.0 1.545421560257092 8.945689644684364E-5 68-69 0.002307987928328566 0.0 0.0 1.7588120410413928 1.0734827573621236E-4 70-71 0.0024153362040647782 0.0 0.0 1.9978229769680698 1.0734827573621236E-4 72-73 0.002504793100511622 0.0 0.0 2.24467033702349 1.4313103431494982E-4 74-75 0.0025584672383797283 0.0 0.0 2.505276167752435 1.4313103431494982E-4 76-77 0.002576358617669097 0.0 0.0 2.7890334432818227 1.4313103431494982E-4 78-79 0.002576358617669097 0.0 0.0 3.117680189448237 1.4313103431494982E-4 80-81 0.00309520861706079 0.0 0.0 3.479604901092877 1.4313103431494982E-4 82-83 0.0031846655135076337 0.0 0.0 3.850940478243725 1.4313103431494982E-4 84-85 0.003220448272086371 0.0 0.0 4.236929095032566 1.4313103431494982E-4 86-87 0.003292013789243846 0.0 0.0 4.641041679041537 1.4313103431494982E-4 88-89 0.0033277965478225834 0.0 0.0 5.083227118178286 1.4313103431494982E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9745 0.0 28.413391 1 GTATCAA 13720 0.0 27.892677 1 GTACATG 10475 0.0 25.523348 1 TACATGG 10745 0.0 24.38032 2 ACATGGG 10995 0.0 22.96271 3 ATCAACG 16900 0.0 22.493267 3 TATCAAC 17130 0.0 22.302073 2 TCAACGC 17200 0.0 22.100943 4 GAGTACT 9490 0.0 21.980534 12-13 CAACGCA 17415 0.0 21.82809 5 AACGCAG 17800 0.0 21.40929 6 GGACCGA 435 0.0 20.728651 6 CATGGGG 6590 0.0 20.596212 4 GTACTTT 10230 0.0 20.506533 14-15 AGTACTT 10135 0.0 20.113377 12-13 TATAACG 120 0.004789792 19.774044 2 AGAGTAC 15750 0.0 19.330809 10-11 ACGCAGA 19835 0.0 19.11707 7 CGCAGAG 20030 0.0 18.88357 8 GTAAGGT 570 0.0 18.317009 4 >>END_MODULE