FastQCFastQC Report
Fri 27 May 2016
SRR939061_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939061_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504452
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146810.5861961019815911No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107150.4278381059010115No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75570.30174265667698963No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27570.11008396247961631No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83000.040.7290731
GTATCAA135050.036.9145281
ATCAACG167150.029.6227973
TCAACGC169500.029.2120974
TATCAAC170950.029.1583392
CAACGCA172450.028.6574295
AACGCAG176350.028.1210216
GTACATG119200.025.8107931
TACATGG120500.025.2050232
ACGCAGA199350.024.8290427
CGCAGAG199800.024.6782938
ACATGGG121550.023.7788583
GAGTACT113800.023.38987512-13
GCAGAGT212300.023.0226279
GTACTTT122350.021.52438214-15
AGTACTT117350.021.49167812-13
CATGGGG53650.021.1961824
CAGAGTA207300.019.51118910-11
AGAGTAC192700.019.50250410-11
ACTTTTT142150.018.6786316-17
Produced by FastQC (version 0.11.5)