FastQCFastQC Report
Fri 27 May 2016
SRR939061_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939061_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504452
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149570.5972164768979401No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119780.4782682998116954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86340.34474607618752523No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31870.127253387168131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83150.044.6612431
GTATCAA137050.035.7813451
ATCAACG172350.028.2202363
TCAACGC175600.027.6979394
TATCAAC179150.027.2550282
CAACGCA179300.027.1528345
AACGCAG184450.026.4461546
ACGCAGA207850.023.3546687
CGCAGAG208950.023.209018
GAGTACT124450.022.53547512-13
TTTACGC1503.059189E-522.1439383
GTACATG120600.021.7916561
GCAGAGT221750.021.6553389
GTACTTT132350.020.97519514-15
TACATGG122150.020.8995482
AGTACTT129400.020.20651412-13
TAAGGTG13050.019.6350215
ACATGGG123500.019.5568523
CAGAGTA217650.019.38244210-11
AGAGTAC202200.019.36146510-11
Produced by FastQC (version 0.11.5)