##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939061_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2504452 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24855297685881 33.0 31.0 34.0 31.0 34.0 2 32.48228315016618 34.0 31.0 34.0 31.0 34.0 3 32.55977834672016 34.0 31.0 34.0 31.0 34.0 4 36.11008476105751 37.0 35.0 37.0 35.0 37.0 5 36.063692975549145 37.0 35.0 37.0 35.0 37.0 6 36.10527133281053 37.0 35.0 37.0 35.0 37.0 7 36.05152783922391 37.0 35.0 37.0 35.0 37.0 8 36.036701042783015 37.0 35.0 37.0 35.0 37.0 9 37.734678484554706 39.0 38.0 39.0 35.0 39.0 10-11 37.651554911014465 39.0 37.0 39.0 35.0 39.0 12-13 37.56477764397161 39.0 37.0 39.0 35.0 39.0 14-15 38.816230656447004 40.0 38.0 41.0 35.0 41.0 16-17 38.73806605197464 40.0 38.0 41.0 35.0 41.0 18-19 38.78221343431617 40.0 38.0 41.0 34.5 41.0 20-21 38.87510501299286 40.0 38.0 41.0 35.0 41.0 22-23 38.824116014201906 40.0 38.0 41.0 35.0 41.0 24-25 38.74523728144919 40.0 38.0 41.0 34.0 41.0 26-27 38.57678605938544 40.0 38.0 41.0 34.0 41.0 28-29 38.38393528803906 40.0 38.0 41.0 34.0 41.0 30-31 38.125215815675446 40.0 37.5 41.0 33.5 41.0 32-33 37.92428343605707 40.0 37.0 41.0 33.0 41.0 34-35 37.82776152228112 40.0 37.0 41.0 33.0 41.0 36-37 37.67942068763945 40.0 37.0 41.0 33.0 41.0 38-39 37.50674758390258 40.0 37.0 41.0 33.0 41.0 40-41 37.281237572131545 40.0 36.0 41.0 32.0 41.0 42-43 37.06061425812912 40.0 36.0 41.0 31.0 41.0 44-45 36.767209153938666 40.0 36.0 41.0 31.0 41.0 46-47 36.382586290334174 39.0 35.0 41.0 30.0 41.0 48-49 35.97327459260549 39.0 35.0 41.0 29.0 41.0 50-51 35.652794303903605 39.0 35.0 40.0 28.0 41.0 52-53 35.36779223558686 38.5 35.0 40.0 27.0 41.0 54-55 35.07893403427177 38.0 34.5 40.0 26.5 41.0 56-57 34.758593297056606 38.0 34.0 40.0 26.0 41.0 58-59 34.41363959061703 38.0 34.0 40.0 25.5 41.0 60-61 34.004929621330334 37.0 33.0 40.0 24.5 41.0 62-63 33.56068313547235 36.5 33.0 39.5 23.0 41.0 64-65 33.09773195892754 36.0 32.0 39.0 22.0 40.0 66-67 32.70652601846631 35.5 32.0 39.0 21.5 40.0 68-69 32.18866322852265 35.0 31.0 38.5 19.5 40.0 70-71 31.681024631336516 35.0 31.0 38.0 17.5 40.0 72-73 31.14714117100268 35.0 30.5 37.0 13.5 39.0 74-75 30.590605250170498 34.0 30.0 36.5 8.0 39.0 76-77 28.674364092424213 32.5 27.0 34.5 7.0 37.0 78-79 29.662963993719984 34.0 29.0 35.0 6.5 37.5 80-81 29.68303904407032 34.0 29.0 35.0 2.0 37.0 82-83 29.34089633181231 34.0 29.0 35.0 2.0 37.0 84-85 28.88987690720365 34.0 29.0 35.0 2.0 36.0 86-87 28.217250919562442 33.5 27.5 35.0 2.0 36.0 88-89 27.5701221265171 33.0 26.0 35.0 2.0 35.0 90-91 26.98672104715922 33.0 25.5 35.0 2.0 35.0 92-93 26.567557693259843 33.0 25.0 35.0 2.0 35.0 94-95 26.071182039024904 32.0 24.5 34.5 2.0 35.0 96-97 25.30125791989625 32.0 21.5 34.0 2.0 35.0 98-99 24.27206191214685 32.0 8.5 34.0 2.0 35.0 100-101 20.60554165142714 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19307125173091322 1101 2 0.1970543105295235 1101 3 0.19741004631142545 1101 4 0.1681616235584542 1101 5 0.15351183451149097 1101 6 0.19979040023125805 1101 7 0.13690619876842192 1101 8 0.2613414152248055 1101 9 0.3601550365011619 1101 10-11 0.34124162304453165 1101 12-13 0.2578800688426597 1101 14-15 0.35233709684788295 1101 16-17 0.3068499846923487 1101 18-19 0.3487493817179299 1101 20-21 0.37529305377384503 1101 22-23 0.32693705653687744 1101 24-25 0.2677500513626825 1101 26-27 0.36767111707816724 1101 28-29 0.21042243022491647 1101 30-31 0.19646685518011253 1101 32-33 0.3222354016503104 1101 34-35 0.46750831142265525 1101 36-37 0.4089257148613754 1101 38-39 0.4693855836796885 1101 40-41 0.43513642530085406 1101 42-43 0.46511279861707777 1101 44-45 0.536662858473079 1101 46-47 0.5631750540307294 1101 48-49 0.44952496497447214 1101 50-51 0.4826747122468831 1101 52-53 0.5056584103804695 1101 54-55 0.45378531944512446 1101 56-57 0.21000294165560973 1101 58-59 0.29508676616713814 1101 60-61 0.30200455126776404 1101 62-63 0.18156760160835006 1101 64-65 0.1388118594098131 1101 66-67 0.132174455216429 1101 68-69 0.026180308071175773 1101 70-71 0.1633457014973736 1101 72-73 -0.046859138441799786 1101 74-75 0.08585476497286137 1101 76-77 0.19315096613274463 1101 78-79 0.306761405920561 1101 80-81 0.09975347623219477 1101 82-83 0.23614241442971107 1101 84-85 0.2557925684754281 1101 86-87 0.34808900024886924 1101 88-89 0.4585464892884126 1101 90-91 0.3640903308869383 1101 92-93 0.28799495720697976 1101 94-95 0.12115958273339089 1101 96-97 0.027155806610895894 1101 98-99 -0.08278124400132825 1101 100-101 -0.5610396225508829 1102 1 0.14377574500817758 1102 2 0.11857780572609755 1102 3 0.17648964951405333 1102 4 0.10791598520269474 1102 5 0.10457894773571041 1102 6 0.14579351598865742 1102 7 0.16293379038148004 1102 8 0.18622994426788608 1102 9 0.30046878399799226 1102 10-11 0.26386625433137567 1102 12-13 0.11002485684473129 1102 14-15 0.14503510021886967 1102 16-17 0.17419508751987678 1102 18-19 0.26129288125009253 1102 20-21 0.20740001833576827 1102 22-23 0.2739753234433806 1102 24-25 0.2603923341166805 1102 26-27 0.14410918258199956 1102 28-29 0.11148028970512769 1102 30-31 0.27501201998989444 1102 32-33 0.21605324675253712 1102 34-35 0.1606803165286621 1102 36-37 0.3132160438468432 1102 38-39 0.22904346067662118 1102 40-41 0.21177363454603437 1102 42-43 0.2851454106339446 1102 44-45 0.2753253377710436 1102 46-47 0.0451186612788419 1102 48-49 0.014682914693551652 1102 50-51 0.19522964520579222 1102 52-53 0.2679777790809368 1102 54-55 0.24627308250806124 1102 56-57 0.07708598717174198 1102 58-59 0.0949312557111952 1102 60-61 0.14336327830134366 1102 62-63 0.12225189517118906 1102 64-65 0.054683239105393966 1102 66-67 -0.05042975442465547 1102 68-69 0.03545000338719717 1102 70-71 0.07562813448302919 1102 72-73 -0.16471151288910235 1102 74-75 -0.021805115746971637 1102 76-77 -0.012142651003209437 1102 78-79 -0.02174957750077411 1102 80-81 -0.08064747046346454 1102 82-83 0.09068891558316139 1102 84-85 0.06525926748065558 1102 86-87 0.2080540936205324 1102 88-89 0.27642111631497457 1102 90-91 0.37660864517578574 1102 92-93 0.4728662350519315 1102 94-95 0.31382411806664123 1102 96-97 0.31837350817111343 1102 98-99 0.0011598245108537242 1102 100-101 -0.4919294126395073 1103 1 0.06215583769105848 1103 2 0.10207118264577275 1103 3 0.11323180288967194 1103 4 0.03690070676709922 1103 5 0.1753802413955654 1103 6 0.13631375825193004 1103 7 0.1848782580430779 1103 8 0.1579402282291653 1103 9 0.12277938618907314 1103 10-11 0.19345820122909885 1103 12-13 0.21903800781505112 1103 14-15 0.26870175837257904 1103 16-17 0.22704158807553654 1103 18-19 0.23174818126216223 1103 20-21 0.1984469030579561 1103 22-23 0.2069879311322893 1103 24-25 0.1806252524820664 1103 26-27 0.15071436635351887 1103 28-29 0.21828858488493807 1103 30-31 0.23820480053824866 1103 32-33 0.3225284725006716 1103 34-35 0.26163034315100475 1103 36-37 0.2912029888407588 1103 38-39 0.23583883164031505 1103 40-41 0.26615682052782574 1103 42-43 0.2665725575242348 1103 44-45 0.24143525706821833 1103 46-47 0.2570277266490635 1103 48-49 0.2707987001918042 1103 50-51 0.21550209077704352 1103 52-53 0.23995544727374352 1103 54-55 0.19646735204994314 1103 56-57 0.23055350792014195 1103 58-59 0.23249637983010984 1103 60-61 0.11687318196562302 1103 62-63 0.05892449382088216 1103 64-65 -0.017936634933796824 1103 66-67 0.06991646365561621 1103 68-69 0.07421829850508743 1103 70-71 0.016810471955206907 1103 72-73 -0.12114829489859247 1103 74-75 -0.15791268624199262 1103 76-77 -0.23848272582435825 1103 78-79 -0.18175679029683067 1103 80-81 -0.13396837487998425 1103 82-83 -0.06415112596006978 1103 84-85 -0.1493804254956892 1103 86-87 0.10221840374600077 1103 88-89 -0.07011165315106282 1103 90-91 0.2351927624750445 1103 92-93 0.06909998691317298 1103 94-95 -0.3355209066518867 1103 96-97 -0.30770463844986295 1103 98-99 -0.40429111841519116 1103 100-101 -0.7570889570570181 1104 1 0.07735587370654429 1104 2 0.07673278713570397 1104 3 0.09356659685107616 1104 4 0.0358678876548808 1104 5 0.1053267433914229 1104 6 0.05445168255938171 1104 7 -0.0017127260800933186 1104 8 0.07401138882822522 1104 9 0.05849324314756643 1104 10-11 0.09162047350617541 1104 12-13 0.012103713568528462 1104 14-15 0.0877819003336171 1104 16-17 0.13307193112305526 1104 18-19 0.12849759601050437 1104 20-21 0.07465004540916453 1104 22-23 0.14693717180577437 1104 24-25 0.13754191414658123 1104 26-27 0.15468338553176153 1104 28-29 0.10910932530331507 1104 30-31 0.20616846241285458 1104 32-33 0.20448283787778365 1104 34-35 0.20755215202881772 1104 36-37 0.24137237417645707 1104 38-39 0.220783795474766 1104 40-41 0.3066669273737759 1104 42-43 0.28634974921666867 1104 44-45 0.3842216552194202 1104 46-47 0.29825176548576593 1104 48-49 0.13389258056035658 1104 50-51 0.16003974196704718 1104 52-53 0.12299965628361775 1104 54-55 0.2664989031169043 1104 56-57 0.40181577569529026 1104 58-59 0.38062282420118976 1104 60-61 0.3195396410430291 1104 62-63 0.31231538940598824 1104 64-65 0.16021153127767462 1104 66-67 0.35574905917169986 1104 68-69 0.3734895476422224 1104 70-71 0.165855873978078 1104 72-73 0.1087642549978618 1104 74-75 0.09122725643335983 1104 76-77 0.09960083760791605 1104 78-79 0.3495046521234073 1104 80-81 0.2573076237994485 1104 82-83 0.40592563204587506 1104 84-85 0.31504040045947335 1104 86-87 0.244449000402561 1104 88-89 0.3072771865500066 1104 90-91 0.5405241799845726 1104 92-93 0.43910096233260276 1104 94-95 0.3510995276348545 1104 96-97 0.4459650019955319 1104 98-99 0.3274555951716529 1104 100-101 0.02813662744431511 1105 1 0.025350771461774002 1105 2 0.07704350075949407 1105 3 0.029075167822789183 1105 4 0.054560491943817624 1105 5 0.12795650721272978 1105 6 0.08384955226316748 1105 7 0.0710117054593411 1105 8 0.06942554646723664 1105 9 0.10465293177980328 1105 10-11 0.06817231543834623 1105 12-13 0.10121401872201119 1105 14-15 0.15756509127168528 1105 16-17 0.09260902233476287 1105 18-19 0.14627314084957277 1105 20-21 0.11929632247849753 1105 22-23 0.126386827113528 1105 24-25 0.024871157931393384 1105 26-27 0.032143928535624866 1105 28-29 0.12451526992724382 1105 30-31 0.0709967704227239 1105 32-33 0.08677761243779258 1105 34-35 0.1306763320900899 1105 36-37 0.044249291606583085 1105 38-39 -0.059258032756929424 1105 40-41 -0.02024541504354005 1105 42-43 -0.06149905862421434 1105 44-45 -0.01763463155290168 1105 46-47 -0.0078925509987684 1105 48-49 -0.109703629823656 1105 50-51 -0.08110710020132927 1105 52-53 -0.16360204519145327 1105 54-55 -0.14062752280424462 1105 56-57 -0.11144842537095201 1105 58-59 -0.11110910444006805 1105 60-61 -0.005725192352691977 1105 62-63 -0.1691141113081116 1105 64-65 -0.21132292159379773 1105 66-67 -0.257577829730927 1105 68-69 -0.348513156111423 1105 70-71 -0.32876623647607417 1105 72-73 -0.23717666217060795 1105 74-75 -0.4042564937892301 1105 76-77 -0.14637587522364726 1105 78-79 0.1325258787699184 1105 80-81 -0.13607708327573675 1105 82-83 -0.1568325237324899 1105 84-85 -0.07236297850576179 1105 86-87 -0.0020273980812994807 1105 88-89 0.1798518940976237 1105 90-91 -0.021054535950494113 1105 92-93 0.013200710491034329 1105 94-95 -0.2829414211500918 1105 96-97 -0.0423754238456695 1105 98-99 0.029187051382837836 1105 100-101 -0.42434435724531383 1106 1 0.11930450394768854 1106 2 0.0778222968920943 1106 3 0.10065699753662471 1106 4 0.02192305728027577 1106 5 0.06657561854863303 1106 6 0.1073930905494791 1106 7 0.1275922239437861 1106 8 0.10266406420977603 1106 9 0.17937712359130842 1106 10-11 0.17162081341891167 1106 12-13 0.1375663974121153 1106 14-15 0.15365081567209415 1106 16-17 0.08842358873432943 1106 18-19 0.07800159177411814 1106 20-21 0.15491605395708063 1106 22-23 0.15756329105789746 1106 24-25 0.19371955969631216 1106 26-27 0.18652301112683034 1106 28-29 0.17358710306121594 1106 30-31 0.2600188686085687 1106 32-33 0.36545665207432876 1106 34-35 0.21547775407687908 1106 36-37 0.24182535261704174 1106 38-39 0.30679415028998847 1106 40-41 0.2120829895026617 1106 42-43 0.2684034303821008 1106 44-45 0.2855642210596159 1106 46-47 0.3400362536576935 1106 48-49 0.3519284395645599 1106 50-51 0.26598714136630264 1106 52-53 0.3816585753253463 1106 54-55 0.23707713045411793 1106 56-57 0.19795123628598077 1106 58-59 0.23139415775039396 1106 60-61 0.35546591218247414 1106 62-63 0.2698703971255796 1106 64-65 0.30087636480093494 1106 66-67 0.40648908496928016 1106 68-69 0.3071330364349336 1106 70-71 0.1289912647972109 1106 72-73 0.025751035970621672 1106 74-75 -0.051220777171486986 1106 76-77 0.17443289388315364 1106 78-79 0.3556560886125055 1106 80-81 0.3524963379811439 1106 82-83 0.3295044837491048 1106 84-85 0.38516430759531417 1106 86-87 0.18888248216445547 1106 88-89 0.4273334689423507 1106 90-91 0.47211057059439554 1106 92-93 0.42110034691165765 1106 94-95 0.17918902129011371 1106 96-97 0.2960099957071449 1106 98-99 0.35949093893971096 1106 100-101 -0.06704555683490199 1107 1 0.047570604595527755 1107 2 0.07475060253956656 1107 3 0.031806818911192636 1107 4 0.03252884225695851 1107 5 0.030038728850783514 1107 6 0.147487006682951 1107 7 0.05512191224918439 1107 8 0.06650143891997118 1107 9 0.1270021517158355 1107 10-11 0.16831949506244115 1107 12-13 0.058175643998005455 1107 14-15 0.12924083324602975 1107 16-17 0.14830850242884708 1107 18-19 0.12200559492238483 1107 20-21 0.13556436270771144 1107 22-23 0.2079438408866423 1107 24-25 0.10402904640715605 1107 26-27 0.14520979069098416 1107 28-29 -0.027470210719947374 1107 30-31 0.19595570677774532 1107 32-33 0.07264616365086596 1107 34-35 0.11043541625714681 1107 36-37 0.09127404465213118 1107 38-39 0.0899558868701078 1107 40-41 0.05844526230405478 1107 42-43 0.16851597895948345 1107 44-45 0.05950767717938987 1107 46-47 0.005810143767675413 1107 48-49 0.09974070086371967 1107 50-51 -0.11272670505369575 1107 52-53 0.05473886732563926 1107 54-55 0.007339702852149799 1107 56-57 0.021403276843180663 1107 58-59 -0.15643952278161066 1107 60-61 0.11191065609827433 1107 62-63 0.08266431627134097 1107 64-65 0.13552865482287046 1107 66-67 -0.02669041402462824 1107 68-69 -0.22690844868796844 1107 70-71 -0.08129568863833825 1107 72-73 -0.20769125587383286 1107 74-75 0.0823910275049009 1107 76-77 -0.017123758288061453 1107 78-79 0.038883590719002825 1107 80-81 0.1338066942172489 1107 82-83 0.016089006333960043 1107 84-85 0.11210619547316725 1107 86-87 0.0989030246358098 1107 88-89 0.20730941437648553 1107 90-91 0.22051771709249124 1107 92-93 0.18787682473017853 1107 94-95 0.0724182348485165 1107 96-97 0.17290746492919595 1107 98-99 0.10241630667889368 1107 100-101 -0.0639762184633561 1108 1 -0.04807073573551435 1108 2 -0.05728266350807587 1108 3 -0.024557008299140648 1108 4 -0.03803425826787077 1108 5 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 29.0 5 75.0 6 136.0 7 393.0 8 921.0 9 1624.0 10 2764.0 11 4132.0 12 6180.0 13 8901.0 14 11101.0 15 14903.0 16 19938.0 17 28260.0 18 27503.0 19 19066.0 20 17494.0 21 17909.0 22 19357.0 23 21204.0 24 24243.0 25 27923.0 26 32771.0 27 38679.0 28 46776.0 29 56751.0 30 67939.0 31 82788.0 32 102193.0 33 130326.0 34 176578.0 35 251985.0 36 363771.0 37 514101.0 38 344566.0 39 21162.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.10464335668818 20.313864379569292 15.46422351431332 22.117268749429215 2 15.84709948523669 21.291404267280825 39.75704066198913 23.10445558549335 3 19.29731534084103 25.429035972739744 30.309385047108112 24.964263639311117 4 13.447253131623205 17.664982199698777 36.55957470935758 32.32818995932044 5 13.710703978355346 37.63981102452752 33.674432570478494 14.97505242663864 6 31.24647627504939 36.67724516181583 17.189229420248424 14.887049142886347 7 27.967555377383956 33.621207353944094 20.54453429333044 17.86670297534151 8 26.043342016536954 35.41018154869808 19.657114610301974 18.889361824463 9 26.483757724244665 17.29244561285263 20.662524176945695 35.561272485957005 10-11 24.977859427930742 27.18914157668025 26.61805856131401 21.214940434075 12-13 26.07576427897201 24.53476848428319 27.70332591720664 21.686141319538166 14-15 22.691542355106048 25.732038852008632 26.55490189071231 25.021516902173012 16-17 21.63959372418301 29.13442184117837 27.209594926064334 22.016389508574285 18-19 21.60873213505318 26.948626055895296 30.408786278666717 21.03385553038481 20-21 22.33702223081137 26.072949291900983 30.532906999215797 21.05712147807185 22-23 21.969756258055654 26.10816657695975 30.460935166655222 21.461141998329374 24-25 21.834213702778385 26.346118754644248 30.515991392889667 21.303676149687696 26-27 21.430111558452293 26.65144562009593 30.47912756091513 21.43931526053664 28-29 21.33089394406441 26.78893426585936 30.622108149806827 21.25806364026941 30-31 21.487077614175192 26.531489892687798 30.427711504866483 21.553720988270527 32-33 21.332990211032193 26.587253419111246 30.36137246790915 21.718383901947412 34-35 22.015203636605413 26.637631486418734 30.018347151100137 21.328817725875712 36-37 22.010945373976927 26.373079292554607 30.015286779382116 21.600688554086343 38-39 21.605303005644906 26.641848431980257 30.16246205727497 21.590386505099865 40-41 21.82167749577049 26.51270510354588 30.20108967919616 21.46452772148747 42-43 21.794536460931052 26.62084632982736 29.770254065069057 21.814363144172532 44-45 21.79006023434895 26.828243261319646 29.64106956398477 21.740626940346633 46-47 21.961068908032782 27.144933467762062 29.018577118500254 21.875420505704902 48-49 22.076622825134518 27.202765971506963 28.794022128331065 21.926589075027454 50-51 22.046996346503224 27.416977779552397 28.641957315977564 21.89406855796682 52-53 22.374200498592206 27.338201598234185 28.44764612131638 21.839951781857224 54-55 21.83603438995836 27.293835138385564 28.70687879025032 22.16325168140575 56-57 21.96793949335024 27.72323047117693 28.378523525306136 21.930306510166695 58-59 22.031900087467786 27.201543873351593 28.559181312679677 22.20737472650094 60-61 21.969573086787282 27.270153412420544 28.847544971992022 21.912728528800145 62-63 21.898975782139075 27.394134206480725 28.787529698324914 21.919360313055282 64-65 21.687787081146272 27.202100934371952 28.722199784020948 22.38791220046083 66-67 21.66759172004025 27.538093564822947 28.542821319618582 22.251493395518214 68-69 21.8549189240127 27.262467062692984 28.67217781334215 22.210436199952163 70-71 21.74507376869173 27.37060033141009 28.563996086965204 22.32032981293298 72-73 21.89369171379607 27.372055842954868 28.472036996516604 22.26221544673246 74-75 21.79860105124794 27.690568635374124 28.114813140758937 22.396017172619 76-77 21.815115876167223 27.40356723026312 28.43575376524523 22.34556312832442 78-79 21.780746107212035 27.568908645808506 28.351778581949887 22.298566665029572 80-81 21.663580069446443 27.859714355991272 28.23166329656569 22.245042277996593 82-83 21.870816796839048 27.737807581788392 28.050093524069595 22.341282097302965 84-85 21.748909541887805 27.598213102107767 28.080574113618468 22.57230324238596 86-87 21.831729440423135 27.50360079004541 28.078188355443697 22.586481414087757 88-89 21.744257283019458 27.480210640271636 28.051741818370562 22.723790258338344 90-91 21.61656440488452 27.58966107332791 28.161973044569038 22.631801477218534 92-93 21.684164839254255 27.338056389182146 28.239650829802287 22.73812794176131 94-95 21.461401536144432 27.18904175444369 28.25610153438756 23.093455175024317 96-97 21.68995885925521 26.98351315647358 28.25670610910213 23.069821875169076 98-99 21.35698803096843 27.109693795553184 28.004930479953156 23.528387693525232 100-101 21.556840918737425 26.465648766569096 28.781119269520456 23.196391045173026 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1944.0 1 1311.5 2 975.5 3 1276.0 4 1018.0 5 913.0 6 1710.5 7 3863.5 8 6244.0 9 6783.5 10 5379.5 11 3978.0 12 3754.5 13 4032.0 14 4293.0 15 4542.5 16 4706.0 17 4884.5 18 5103.5 19 5357.5 20 5905.0 21 6732.5 22 7882.5 23 9567.0 24 11755.0 25 14160.5 26 17332.5 27 21065.0 28 24233.0 29 27769.5 30 31972.5 31 37085.5 32 43197.5 33 47033.5 34 50495.5 35 54321.5 36 58484.0 37 63699.0 38 66519.0 39 67440.5 40 68837.0 41 73607.5 42 78523.5 43 81767.0 44 84877.0 45 88463.5 46 89148.0 47 90565.0 48 98569.0 49 99464.5 50 93837.0 51 89412.5 52 84179.0 53 81861.5 54 79872.5 55 73306.5 56 64916.5 57 57988.5 58 52530.0 59 45766.0 60 38286.0 61 32413.0 62 27402.5 63 21072.0 64 15728.5 65 12501.0 66 9334.5 67 7262.5 68 5703.5 69 4069.0 70 3078.5 71 2540.0 72 2151.5 73 1955.0 74 1684.0 75 1386.0 76 995.0 77 602.5 78 380.0 79 241.0 80 156.5 81 95.5 82 65.5 83 47.0 84 24.5 85 13.5 86 5.0 87 5.5 88 5.5 89 2.5 90 1.5 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3152785519546791 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010181868129235459 16-17 0.0017968002581003747 18-19 5.9893341936679164E-5 20-21 0.0 22-23 0.0 24-25 4.3921784086898056E-4 26-27 9.982223656113194E-4 28-29 0.0 30-31 0.0030745248860828637 32-33 0.0 34-35 0.009922330314176515 36-37 0.02613346153170434 38-39 0.0205034873896565 40-41 0.012797210727137114 42-43 0.010022152550737646 44-45 0.002675235939838336 46-47 0.0010780801548602248 48-49 6.18897866679018E-4 50-51 7.985778924890555E-5 52-53 1.9964447312226387E-5 54-55 0.0 56-57 0.0 58-59 0.0040527828043819564 60-61 0.010161903681923231 62-63 0.0040727472516941835 64-65 0.0016969780215392429 66-67 0.0036734583054496553 68-69 0.002914809307585053 70-71 7.985778924890555E-5 72-73 0.0 74-75 0.0 76-77 0.205474091737434 78-79 5.190756301178861E-4 80-81 0.0030345959914584108 82-83 6.588267613034707E-4 84-85 0.0 86-87 0.10263722363215584 88-89 0.3788253877494957 90-91 0.36822426622670346 92-93 0.0 94-95 0.0 96-97 1.9964447312226387E-5 98-99 0.0012178312860458096 100-101 5.989334193667916E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2504452.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.62062458616438 #Duplication Level Percentage of deduplicated Percentage of total 1 72.45564698521869 35.228388112159706 2 14.751755301456338 14.344791129981374 3 5.371634247980207 7.835166365556871 4 2.485557764498175 4.833974838195669 5 1.3176919522932942 3.2033502866331136 6 0.793467639599104 2.3147335335730803 7 0.5260630410344946 1.7904259538755902 8 0.3733826298827304 1.4523277339618397 9 0.2765725560212271 1.2102417375429597 >10 1.4452296132650813 13.032435917296818 >50 0.12381993139177892 4.16267665170711 >100 0.07260817861679454 6.503467376450183 >500 0.0048454920723419925 1.606529950451012 >1k 0.0014782857169847615 1.0611970264003625 >5k 8.212698427693119E-5 0.3447612554914483 >10k+ 1.6425396855386238E-4 1.0755321307229744 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14957 0.5972164768979401 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11978 0.4782682998116954 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8634 0.34474607618752523 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3187 0.127253387168131 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.985778924890555E-5 0.0 14-15 0.0 0.0 0.0 1.597155784978111E-4 0.0 16-17 1.9964447312226387E-5 0.0 0.0 1.9964447312226388E-4 0.0 18-19 5.989334193667916E-5 0.0 0.0 3.5936005162007494E-4 0.0 20-21 7.985778924890555E-5 0.0 0.0 4.192533935567541E-4 0.0 22-23 7.985778924890555E-5 0.0 0.0 5.390400774301124E-4 0.0 24-25 7.985778924890555E-5 0.0 0.0 8.385067871135082E-4 0.0 26-27 7.985778924890555E-5 0.0 0.0 0.0011978668387335831 0.0 28-29 1.3975113118558472E-4 0.0 0.0 0.0027550937290872412 0.0 30-31 1.597155784978111E-4 0.0 0.0 0.007865992241017198 0.0 32-33 1.597155784978111E-4 0.0 0.0 0.01718938913582692 0.0 34-35 1.7968002581003747E-4 0.0 0.0 0.02940763089090947 0.0 36-37 1.9964447312226388E-4 0.0 0.0 0.050310407226810495 0.0 38-39 1.9964447312226388E-4 0.0 0.0 0.08273266966186615 0.0 40-41 1.9964447312226388E-4 0.0 0.0 0.11838917256150247 0.0 42-43 1.9964447312226388E-4 0.0 0.0 0.156441409138606 0.0 44-45 1.9964447312226388E-4 0.0 0.0 0.205653771763244 0.0 46-47 1.9964447312226388E-4 0.0 0.0 0.2644091402031263 0.0 48-49 1.9964447312226388E-4 0.0 0.0 0.33087078530552794 0.0 50-51 1.9964447312226388E-4 0.0 0.0 0.4036811246532176 0.0 52-53 1.9964447312226388E-4 0.0 0.0 0.4791866643880578 0.0 54-55 1.9964447312226388E-4 0.0 0.0 0.5666109791682972 0.0 56-57 1.9964447312226388E-4 0.0 0.0 0.6730813766844004 0.0 58-59 1.9964447312226388E-4 0.0 0.0 0.791390691456654 0.0 60-61 1.9964447312226388E-4 0.0 0.0 0.9223375013775469 0.0 62-63 1.9964447312226388E-4 0.0 0.0 1.056837982920016 0.0 64-65 1.9964447312226388E-4 0.0 0.0 1.2045948574778036 0.0 66-67 1.9964447312226388E-4 0.0 0.0 1.3843946699717145 0.0 68-69 1.9964447312226388E-4 0.0 0.0 1.5820426983627556 0.0 70-71 1.9964447312226388E-4 0.0 0.0 1.803328632371473 0.0 72-73 1.9964447312226388E-4 0.0 0.0 2.036134052479345 0.0 74-75 1.9964447312226388E-4 0.0 0.0 2.282076078918662 0.0 76-77 1.9964447312226388E-4 0.0 0.0 2.551536224291781 0.0 78-79 1.9964447312226388E-4 0.0 0.0 2.8550956456741834 0.0 80-81 1.9964447312226388E-4 0.0 0.0 3.190897649465831 0.0 82-83 1.9964447312226388E-4 0.0 0.0 3.54303057115888 0.0 84-85 1.9964447312226388E-4 0.0 0.0 3.9100370060995377 0.0 86-87 1.9964447312226388E-4 0.0 0.0 4.303136973677276 0.0 88-89 1.9964447312226388E-4 0.0 0.0 4.723089122889958 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8315 0.0 44.661243 1 GTATCAA 13705 0.0 35.781345 1 ATCAACG 17235 0.0 28.220236 3 TCAACGC 17560 0.0 27.697939 4 TATCAAC 17915 0.0 27.255028 2 CAACGCA 17930 0.0 27.152834 5 AACGCAG 18445 0.0 26.446154 6 ACGCAGA 20785 0.0 23.354668 7 CGCAGAG 20895 0.0 23.20901 8 GAGTACT 12445 0.0 22.535475 12-13 TTTACGC 150 3.059189E-5 22.143938 3 GTACATG 12060 0.0 21.791656 1 GCAGAGT 22175 0.0 21.655338 9 GTACTTT 13235 0.0 20.975195 14-15 TACATGG 12215 0.0 20.899548 2 AGTACTT 12940 0.0 20.206514 12-13 TAAGGTG 1305 0.0 19.635021 5 ACATGGG 12350 0.0 19.556852 3 CAGAGTA 21765 0.0 19.382442 10-11 AGAGTAC 20220 0.0 19.361465 10-11 >>END_MODULE