FastQCFastQC Report
Fri 27 May 2016
SRR939060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2486201
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142830.5744909603044966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104730.4212451044786805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74250.2986484198180276No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26690.10735254309687753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75600.041.2052421
GTATCAA126650.036.8943861
ATCAACG158350.029.3767953
TCAACGC160900.028.8823624
TATCAAC162300.028.6898652
CAACGCA162850.028.5068555
AACGCAG166900.027.8300386
GTACATG124350.025.318511
TACATGG123800.024.7558082
ACGCAGA188500.024.6410277
CGCAGAG189100.024.5378028
ACATGGG126000.023.1214733
GAGTACT104800.022.99674612-13
GCAGAGT202350.022.8140569
GTACTTT112450.021.1594314-15
AGTACTT109100.021.07047712-13
CATGGGG54350.020.1340964
AGAGTAC181700.019.79068810-11
CATGGGA68050.019.3524684
CAGAGTA198000.019.07012410-11
Produced by FastQC (version 0.11.5)