FastQCFastQC Report
Fri 27 May 2016
SRR939060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2486201
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145820.586517341116024No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117120.4710801741291231No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82410.3314695794909583No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30650.12328045882050566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79400.041.7068521
GTATCAA133700.037.8117751
ATCAACG171700.029.15683
TCAACGC173850.028.850814
TATCAAC176450.028.4794752
CAACGCA177600.028.2149095
AACGCAG181650.027.5597176
ACGCAGA205700.024.429777
CGCAGAG205800.024.3256688
GCAGAGT220500.022.5761979
GTACATG119700.022.4107991
TACATGG119900.021.9254382
GAGTACT123700.021.86743212-13
AGTACTT126700.021.83657612-13
ACATGGG120650.021.0025313
AGAGTAC196900.020.55871410-11
GTACTTT130750.020.54316514-15
ATTCGCG3150.019.60558980-81
CGTAGAC1951.04243045E-519.4676283
TCGCGGA2950.019.4156982-83
Produced by FastQC (version 0.11.5)