##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939060_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2486201 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.257889446589395 33.0 31.0 34.0 31.0 34.0 2 32.503481818243976 34.0 31.0 34.0 31.0 34.0 3 32.599782157597076 34.0 31.0 34.0 31.0 34.0 4 36.13426388292821 37.0 35.0 37.0 35.0 37.0 5 36.08755607450886 37.0 35.0 37.0 35.0 37.0 6 36.132714531126005 37.0 36.0 37.0 35.0 37.0 7 36.083224968536335 37.0 35.0 37.0 35.0 37.0 8 36.049471864905534 37.0 35.0 37.0 35.0 37.0 9 37.747600455474036 39.0 38.0 39.0 35.0 39.0 10-11 37.63555842830085 39.0 37.0 39.0 35.0 39.0 12-13 37.52225141893194 39.0 37.0 39.0 35.0 39.0 14-15 38.83488925473041 40.0 38.0 41.0 35.0 41.0 16-17 38.95276870212827 40.0 38.0 41.0 35.5 41.0 18-19 38.99651617065555 40.0 38.5 41.0 35.0 41.0 20-21 38.957812139887324 40.0 38.0 41.0 35.0 41.0 22-23 38.86696972610018 40.0 38.0 41.0 35.0 41.0 24-25 38.76618282270822 40.0 38.0 41.0 34.5 41.0 26-27 38.57729644546036 40.0 38.0 41.0 34.0 41.0 28-29 38.3804855279199 40.0 38.0 41.0 34.0 41.0 30-31 38.116995568741224 40.0 37.5 41.0 33.5 41.0 32-33 37.947496803355804 40.0 37.0 41.0 33.0 41.0 34-35 37.79381313095763 40.0 37.0 41.0 33.0 41.0 36-37 37.58811073601853 40.0 37.0 41.0 33.0 41.0 38-39 37.4123457837882 40.0 36.5 41.0 32.5 41.0 40-41 37.16179906612538 40.0 36.0 41.0 31.5 41.0 42-43 36.90104018138517 39.5 36.0 41.0 31.0 41.0 44-45 36.548873562515666 39.0 35.0 41.0 30.5 41.0 46-47 36.12738048934901 39.0 35.0 40.5 30.0 41.0 48-49 35.6768889562831 39.0 35.0 40.0 28.5 41.0 50-51 35.33583869526237 38.5 35.0 40.0 27.0 41.0 52-53 34.99390174004435 38.0 34.0 40.0 26.0 41.0 54-55 34.66731692248535 38.0 34.0 40.0 26.0 41.0 56-57 34.32137204514036 38.0 34.0 40.0 24.5 41.0 58-59 33.89279426723745 37.0 33.0 40.0 23.0 41.0 60-61 33.43124288824596 37.0 33.0 39.5 22.5 41.0 62-63 32.95300319644309 36.0 32.0 39.0 21.0 40.0 64-65 32.39849694373062 35.5 31.0 39.0 18.5 40.0 66-67 31.889249903768842 35.0 31.0 38.5 17.0 40.0 68-69 31.313161928580996 35.0 30.5 38.0 12.0 40.0 70-71 30.710375589101606 34.5 29.5 37.0 8.0 39.0 72-73 30.014943683153533 34.0 29.0 36.5 7.0 39.0 74-75 29.372743796660046 34.0 28.0 36.0 2.0 39.0 76-77 27.4789691179434 31.5 26.0 34.0 2.0 36.0 78-79 28.460056729122062 33.0 27.5 35.0 2.0 37.0 80-81 28.432281219418705 33.0 27.5 35.0 2.0 37.0 82-83 27.933316131720645 33.0 26.5 35.0 2.0 36.0 84-85 27.309547981036125 33.0 26.0 35.0 2.0 36.0 86-87 26.41516152555646 32.0 24.5 34.5 2.0 35.0 88-89 25.55047942624108 31.5 21.5 34.0 2.0 35.0 90-91 24.78503990626663 31.0 19.0 34.0 2.0 35.0 92-93 24.100846633075925 31.0 15.0 34.0 2.0 35.0 94-95 23.26039326667474 30.5 4.5 34.0 2.0 35.0 96-97 22.094753803091542 29.5 2.0 34.0 2.0 35.0 98-99 20.462775535847666 29.0 2.0 34.0 2.0 35.0 100-101 15.030134932774946 12.5 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21149751019084562 1101 2 0.17755912297737098 1101 3 0.2024203502448998 1101 4 0.1382441465256079 1101 5 0.1526314214744815 1101 6 0.14581490806904895 1101 7 0.19551146959003063 1101 8 0.1604339496483007 1101 9 0.09584373456080897 1101 10-11 0.2327646058265671 1101 12-13 0.266344644154195 1101 14-15 0.3754203889634269 1101 16-17 0.28626222351437747 1101 18-19 0.27976159265825373 1101 20-21 0.2712423413556593 1101 22-23 0.2931047570740333 1101 24-25 0.2858929860172097 1101 26-27 0.27957170837049716 1101 28-29 0.30654341629190185 1101 30-31 0.2544352886050447 1101 32-33 0.33526031123904687 1101 34-35 0.28275536653934097 1101 36-37 0.25485516262682495 1101 38-39 0.15356918404046382 1101 40-41 0.3779702401063929 1101 42-43 0.41943426807508644 1101 44-45 0.44707314041898627 1101 46-47 0.38927449892014465 1101 48-49 0.2657851388719976 1101 50-51 0.24043853156782546 1101 52-53 0.3015983132876059 1101 54-55 0.32353814685903615 1101 56-57 0.2827604656470939 1101 58-59 0.29217356095451663 1101 60-61 0.26751129355694303 1101 62-63 0.16871038437227526 1101 64-65 0.3097151690925912 1101 66-67 0.28020984460721365 1101 68-69 0.2295694800377781 1101 70-71 0.10149465408658642 1101 72-73 0.2820970753291441 1101 74-75 0.021993510644851 1101 76-77 0.23577204891451586 1101 78-79 0.3137999264035294 1101 80-81 0.35595533955208225 1101 82-83 0.378595777321717 1101 84-85 0.3541849885331061 1101 86-87 0.4376005402986536 1101 88-89 0.3721952779653286 1101 90-91 0.2695355521274081 1101 92-93 0.15804603660296834 1101 94-95 0.029233582045314677 1101 96-97 -0.10432924755068029 1101 98-99 -0.2581178566110154 1101 100-101 -1.1286861366862606 1102 1 0.05314098672707246 1102 2 0.07268807100762587 1102 3 0.1014530099909905 1102 4 0.028509403450442505 1102 5 0.08722830169482165 1102 6 0.12011792622789841 1102 7 0.20686458047531886 1102 8 0.1849828411225829 1102 9 0.19499089664090974 1102 10-11 0.11633505440991598 1102 12-13 0.17690932468246245 1102 14-15 0.2919646471825459 1102 16-17 0.28275160240055897 1102 18-19 0.1718092010929908 1102 20-21 0.30683058687319686 1102 22-23 0.21672178357109573 1102 24-25 0.2309015021282974 1102 26-27 0.22665547705124567 1102 28-29 0.23463411031917047 1102 30-31 0.13618750146488878 1102 32-33 0.2220993925213932 1102 34-35 0.22003722425791494 1102 36-37 0.3133289487487332 1102 38-39 0.33533489181823484 1102 40-41 0.2486682749600888 1102 42-43 0.22873318664143483 1102 44-45 0.10203967915656165 1102 46-47 0.04557243399323596 1102 48-49 -0.019758941459564028 1102 50-51 -0.03081913344332321 1102 52-53 0.12358378375929391 1102 54-55 0.0498960729764093 1102 56-57 0.04242252018701009 1102 58-59 -0.16563018204499969 1102 60-61 -0.27344363546183814 1102 62-63 -0.32650239572394213 1102 64-65 -0.18335478161007046 1102 66-67 -0.2148340445463468 1102 68-69 -0.24995361774108105 1102 70-71 -0.3399852476867693 1102 72-73 -0.3916356803126533 1102 74-75 -0.40514599613634417 1102 76-77 -0.2629843739507791 1102 78-79 -0.12401804760034807 1102 80-81 -0.01636126758018719 1102 82-83 -0.208603168438934 1102 84-85 -0.297222776769825 1102 86-87 -0.09972507366899563 1102 88-89 -0.021968987900912396 1102 90-91 -0.011382224973349508 1102 92-93 -0.4119068129716368 1102 94-95 -0.5498187271191668 1102 96-97 -1.02435683034507 1102 98-99 -1.0405976704629332 1102 100-101 -1.2143961431679102 1103 1 0.14777147449906636 1103 2 0.06119997951473266 1103 3 0.11895418870725649 1103 4 0.09097380703635594 1103 5 0.15376550704639413 1103 6 0.11616830746191198 1103 7 0.08855680846559721 1103 8 0.023250916746725636 1103 9 0.17146539510428482 1103 10-11 0.14493802436633274 1103 12-13 0.10467485452462455 1103 14-15 0.00609020314031028 1103 16-17 0.1415910346883109 1103 18-19 0.19721227302778033 1103 20-21 0.25722992041844606 1103 22-23 0.10962509451766778 1103 24-25 0.1441069239779793 1103 26-27 0.08620111768047423 1103 28-29 0.11195773391768427 1103 30-31 0.15507351858057916 1103 32-33 0.14985491246805083 1103 34-35 0.16964649105145924 1103 36-37 0.11415368242423085 1103 38-39 0.17587102590670867 1103 40-41 0.2904490680543219 1103 42-43 0.2728786643340797 1103 44-45 0.15501194398325424 1103 46-47 0.24841775700235047 1103 48-49 0.22037855364784775 1103 50-51 0.2186503420953514 1103 52-53 0.2314668624599534 1103 54-55 -0.01079576197226828 1103 56-57 -0.018435397313474766 1103 58-59 -0.034188610009287856 1103 60-61 0.015286080199928165 1103 62-63 0.027539232367168154 1103 64-65 7.447649620999641E-4 1103 66-67 0.08881981245024306 1103 68-69 -0.015061205732148863 1103 70-71 -0.08189745640902402 1103 72-73 -0.3020562062366494 1103 74-75 -0.15899194098468072 1103 76-77 -0.21201214752939634 1103 78-79 -0.054687468554455165 1103 80-81 0.1158448357595212 1103 82-83 0.08874608866339173 1103 84-85 -0.12315539260474395 1103 86-87 -0.483165458408525 1103 88-89 -0.23827353468144352 1103 90-91 0.11622902990800554 1103 92-93 -0.13288393811330934 1103 94-95 -0.2906708061948926 1103 96-97 -0.4899594587393423 1103 98-99 -0.870422807802786 1103 100-101 -1.2282721054457202 1104 1 0.060586217620922866 1104 2 -0.0016969671755333593 1104 3 0.11946155137481895 1104 4 0.03491814416392458 1104 5 -0.05540932456047898 1104 6 0.020165996788513496 1104 7 0.07556588050268687 1104 8 0.06404563937378072 1104 9 0.04642643654177192 1104 10-11 0.1618069817831227 1104 12-13 0.054373441440468184 1104 14-15 0.08066109810962985 1104 16-17 0.06382193217341126 1104 18-19 0.12841966397785143 1104 20-21 0.17924248971261392 1104 22-23 0.15536004329267428 1104 24-25 0.11084953146555421 1104 26-27 0.06367004897799688 1104 28-29 0.11803967629347056 1104 30-31 0.0969148945369156 1104 32-33 0.041288916062249825 1104 34-35 0.062274389308122124 1104 36-37 0.1019112796443693 1104 38-39 0.0505759823973051 1104 40-41 -0.03265251825020954 1104 42-43 0.010482830917290187 1104 44-45 0.050783768189404555 1104 46-47 0.08410606366492601 1104 48-49 -0.056357135439988326 1104 50-51 -0.11915104094363471 1104 52-53 -0.1706316720571337 1104 54-55 -0.2837260307522982 1104 56-57 -0.1925445181439045 1104 58-59 -0.15408624012687966 1104 60-61 -0.14472665093751402 1104 62-63 -0.0580768657515236 1104 64-65 0.011576246845670823 1104 66-67 -0.21861011621853166 1104 68-69 -0.19240318619718622 1104 70-71 -0.36642235423543923 1104 72-73 -0.6163104932809347 1104 74-75 -0.7215757169166572 1104 76-77 -0.5585204187627149 1104 78-79 -0.47063151564604055 1104 80-81 -0.4039800650313481 1104 82-83 -0.49722013576516844 1104 84-85 -0.5635632136413236 1104 86-87 -0.5678306270900855 1104 88-89 -0.4878578097637458 1104 90-91 -0.4848639330338891 1104 92-93 -0.6738644400037472 1104 94-95 -0.8058439144111631 1104 96-97 -0.7863538988617336 1104 98-99 -0.7770970204792818 1104 100-101 -1.0743785726872783 1105 1 -0.02230210642356667 1105 2 0.07235335579627389 1105 3 0.020424636979832655 1105 4 -0.019369102530568227 1105 5 -0.04043835704420928 1105 6 0.054690167205691864 1105 7 0.05637412619444859 1105 8 0.04557678225091166 1105 9 0.10138994189354378 1105 10-11 0.131725131166327 1105 12-13 0.03634765033444154 1105 14-15 0.10158430777313043 1105 16-17 0.11124748542295748 1105 18-19 0.10230208824854259 1105 20-21 0.11064887452710792 1105 22-23 0.0997144494676121 1105 24-25 0.05333839651697758 1105 26-27 0.03141367698909647 1105 28-29 -0.047585396579229666 1105 30-31 -0.008319114277760775 1105 32-33 0.012437156623782641 1105 34-35 0.1263149181890313 1105 36-37 0.14441063836460444 1105 38-39 -0.0563486730605689 1105 40-41 0.14167646992692795 1105 42-43 0.17621867029419747 1105 44-45 0.12044423970492346 1105 46-47 0.11250309745453535 1105 48-49 0.003176945664414177 1105 50-51 0.021260077681461098 1105 52-53 -0.035140918145323496 1105 54-55 -0.0375562198715258 1105 56-57 -7.785502778503428E-4 1105 58-59 -0.15726931966889168 1105 60-61 -0.3462925395180676 1105 62-63 -0.38634214569928105 1105 64-65 -0.29550348171810725 1105 66-67 -0.46791636811322235 1105 68-69 -0.5927541411210449 1105 70-71 -0.6268577248951388 1105 72-73 -0.7562727160731804 1105 74-75 -0.7447667250084464 1105 76-77 -0.8871471473031605 1105 78-79 -0.8301096305167377 1105 80-81 -0.42692622085066745 1105 82-83 -0.3705265742139723 1105 84-85 -0.7883317699456889 1105 86-87 -0.8655301816921401 1105 88-89 -0.8328430020310797 1105 90-91 -0.6338973495392644 1105 92-93 -0.704983997870503 1105 94-95 -0.9994287848726522 1105 96-97 -1.1635429947738665 1105 98-99 -1.1505258850643152 1105 100-101 -1.288239543235047 1106 1 0.023019041880445457 1106 2 0.006455296831532564 1106 3 0.02192392090393014 1106 4 0.0638813244737122 1106 5 0.0587157782941361 1106 6 0.10497395630373063 1106 7 0.06904742243270334 1106 8 0.090336295201908 1106 9 0.0658832888440628 1106 10-11 0.060851225089663785 1106 12-13 0.0854076539735118 1106 14-15 0.05843724955381191 1106 16-17 0.04757193970855411 1106 18-19 -0.03254102069477227 1106 20-21 0.013513288195930784 1106 22-23 0.08802484049201098 1106 24-25 0.057455649544159826 1106 26-27 -0.004330506361547748 1106 28-29 0.06969367639594282 1106 30-31 -0.07994596389595188 1106 32-33 -0.029658348532557 1106 34-35 -0.022080991080635215 1106 36-37 -0.018294866615640615 1106 38-39 0.1078841792693197 1106 40-41 0.016841365479621118 1106 42-43 -0.03585938784882359 1106 44-45 0.08789158318070633 1106 46-47 -0.054106892097799175 1106 48-49 -0.008107302720013365 1106 50-51 0.006008798135987092 1106 52-53 0.03659635096420999 1106 54-55 -0.004611959337289306 1106 56-57 -0.11835036329986792 1106 58-59 -0.18458310324962923 1106 60-61 -0.1504199173889802 1106 62-63 -0.14940686252648305 1106 64-65 -0.0539837720223133 1106 66-67 -0.12250585705806571 1106 68-69 -0.301781906670751 1106 70-71 -0.6090060846704546 1106 72-73 -0.6759986629661725 1106 74-75 -0.6857561276720112 1106 76-77 -0.7414403745163902 1106 78-79 -0.901413659030645 1106 80-81 -0.6616189978751983 1106 82-83 -0.7676513753855154 1106 84-85 -0.8886983186359174 1106 86-87 -1.0871195400411757 1106 88-89 -1.0926635501813138 1106 90-91 -0.8810154374992116 1106 92-93 -0.9961352512968098 1106 94-95 -1.1568310361096437 1106 96-97 -1.5811557473813806 1106 98-99 -1.4960928543024004 1106 100-101 -1.768854319343939 1107 1 0.0013701486002659635 1107 2 0.05195914110714028 1107 3 0.006959212148977656 1107 4 0.020019956888276624 1107 5 0.01794842794831908 1107 6 0.04114876011507107 1107 7 0.043472626571123385 1107 8 0.049522098070632126 1107 9 0.07814153103910115 1107 10-11 0.09927836556187941 1107 12-13 0.06431524372059272 1107 14-15 0.06443186359308584 1107 16-17 0.08898414315419956 1107 18-19 0.13818570071165226 1107 20-21 0.10635119547904992 1107 22-23 0.10374655071198902 1107 24-25 0.04041655093309515 1107 26-27 0.05694047531994073 1107 28-29 0.03572194914077187 1107 30-31 0.094249194133873 1107 32-33 -0.02799386804921511 1107 34-35 -0.04500149066610959 1107 36-37 0.03843375137579841 1107 38-39 -0.014483528247737354 1107 40-41 -0.010596299903731676 1107 42-43 -0.1460217332544289 1107 44-45 -0.029204441372684187 1107 46-47 0.006141977900952611 1107 48-49 0.016260478616224816 1107 50-51 -0.13818383300661452 1107 52-53 -0.2402053338165402 1107 54-55 -0.41324582189734116 1107 56-57 -0.38765194870865827 1107 58-59 -0.3106730008113274 1107 60-61 -0.3398601550867255 1107 62-63 -0.3962581907038256 1107 64-65 -0.4958589778642519 1107 66-67 -0.580596438866749 1107 68-69 -0.757052453954774 1107 70-71 -0.785062692622482 1107 72-73 -1.079203621911354 1107 74-75 -1.1028408664089078 1107 76-77 -0.8566210043643245 1107 78-79 -0.7720735882778023 1107 80-81 -0.8214538416624642 1107 82-83 -0.9299261559154246 1107 84-85 -1.0519309739685028 1107 86-87 -0.9928244138103857 1107 88-89 -1.1251762839893615 1107 90-91 -0.694614917357935 1107 92-93 -0.5485277024341926 1107 94-95 -0.6422819151256931 1107 96-97 -0.924364525049377 1107 98-99 -1.050448966530265 1107 100-101 -1.0821198384063209 1108 1 -0.11130124361079652 1108 2 -0.061145349984570885 1108 3 -0.08041660547055329 1108 4 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2316 8 -0.3608025714040295 2316 9 -0.470796906218645 2316 10-11 -0.5043672007217026 2316 12-13 -0.5034673363957225 2316 14-15 -0.6327458109455364 2316 16-17 -0.658280479540295 2316 18-19 -0.6053955122582195 2316 20-21 -0.745649112607154 2316 22-23 -0.5792727830877311 2316 24-25 -0.6234290044506849 2316 26-27 -0.6938978639508164 2316 28-29 -0.7496583315624648 2316 30-31 -0.8227269953625012 2316 32-33 -0.8851693570665518 2316 34-35 -0.7392118890760742 2316 36-37 -0.8664782772217166 2316 38-39 -0.8231653308078606 2316 40-41 -0.8190136710719784 2316 42-43 -0.7704971803409038 2316 44-45 -0.9378666131691205 2316 46-47 -0.8378223686281885 2316 48-49 -1.0490013835368828 2316 50-51 -0.7873456605695424 2316 52-53 -0.6949988270803544 2316 54-55 -0.7795451671307347 2316 56-57 -0.6701437821997374 2316 58-59 -0.5048272318804266 2316 60-61 -0.3329817412524321 2316 62-63 -0.21291950942401883 2316 64-65 -0.18986625626184406 2316 66-67 -0.24210242986440278 2316 68-69 0.09824420970518233 2316 70-71 -0.0018317859036471873 2316 72-73 0.1772552052033447 2316 74-75 0.00298340878214276 2316 76-77 -0.03969845189790888 2316 78-79 0.05671445397338459 2316 80-81 -0.09466958319926988 2316 82-83 -0.05858426253104909 2316 84-85 -0.05279753593775993 2316 86-87 -0.004044396259477168 2316 88-89 -0.15324688578071033 2316 90-91 -0.4087305208676675 2316 92-93 -0.24225150259412942 2316 94-95 0.0277716607319185 2316 96-97 -0.02040344846047759 2316 98-99 0.27794256766118863 2316 100-101 1.0376934413326158 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 13.0 4 30.0 5 76.0 6 173.0 7 385.0 8 883.0 9 1736.0 10 2755.0 11 4503.0 12 6637.0 13 9324.0 14 11969.0 15 16216.0 16 21524.0 17 30628.0 18 30746.0 19 22694.0 20 21047.0 21 22575.0 22 23938.0 23 26530.0 24 30192.0 25 34587.0 26 39710.0 27 47027.0 28 57064.0 29 67898.0 30 81373.0 31 99572.0 32 122892.0 33 157425.0 34 211470.0 35 292693.0 36 392827.0 37 428313.0 38 165194.0 39 3577.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.1981407895587 20.271128125605735 15.406853718420882 22.12387736641468 2 15.819557630296183 21.22366614766867 39.83704455110427 23.11973167093087 3 19.325830855992738 25.33990614596326 30.248680617536554 25.08558238050745 4 13.389906930292442 17.621744983611542 36.5639785359269 32.42436955016911 5 13.719405631322648 37.60552746942022 33.621255883977206 15.053811015279939 6 31.26135014827844 36.63806747724742 17.15858854533483 14.941993829139316 7 27.951440772487825 33.60251242759536 20.543632634690436 17.902414165226382 8 26.06265543292759 35.45015065153622 19.641493185788278 18.845700729747918 9 26.48719874217732 17.28826430365043 20.675641269551413 35.54889568462084 10-11 24.97635951397333 27.149916680107523 26.619911262202855 21.253812543716297 12-13 26.06498830947297 24.547633115745672 27.666025393763416 21.721353181017946 14-15 22.72542889636802 25.67813440512594 26.57167478991343 25.02476190859261 16-17 21.676817544836286 29.078223570845918 27.197529036794148 22.04742984752365 18-19 21.56732299600877 26.879926441989205 30.480982832844166 21.07176772915786 20-21 22.337212477993535 26.104003658593978 30.47209376876608 21.08669009464641 22-23 21.983459905293255 26.044213641616267 30.49202377442532 21.48030267866516 24-25 21.865703053998683 26.315386194872936 30.521524921873727 21.29738582925465 26-27 21.475407297373994 26.625735309349313 30.457660118896424 21.441197274380272 28-29 21.339675006033303 26.77188078191618 30.620907408897114 21.267536803153405 30-31 21.49337483172117 26.48991372781203 30.436778844510158 21.57993259595664 32-33 21.331232670588182 26.553721855386105 30.325905061118384 21.789140412907333 34-35 22.007293882061322 26.609354528446023 30.06702953716513 21.31632205232753 36-37 22.039849603944045 26.317459110493953 30.033245888619938 21.60944539694207 38-39 21.56969550802068 26.65996977548451 30.147938402815587 21.622396313679218 40-41 21.827441693857327 26.499254121439126 30.203626757822676 21.469677426880875 42-43 21.796112335862773 26.572295198917615 29.800463919526678 21.831128545692934 44-45 21.76311670279064 26.79605062437792 29.658971861513216 21.781860811318225 46-47 21.977810028486637 27.125818256707955 29.032335268644026 21.864036446161382 48-49 22.096332777181583 27.16679967113572 28.766433158014138 21.970434393668558 50-51 22.09786395708125 27.37283131672369 28.635729587408182 21.89357513878688 52-53 22.380028807003136 27.356798585472376 28.431530676723245 21.83164193080125 54-55 21.81696089736912 27.288240170444787 28.682234461332772 22.212564470853323 56-57 21.958318702565645 27.692875751297464 28.398551362943998 21.9502541831929 58-59 22.037023741217133 27.117104017578907 28.645846217150417 22.200026024053546 60-61 21.98196404473593 27.230370714499756 28.888500067373386 21.899165173390934 62-63 21.85887455046334 27.364871523318392 28.817205252575146 21.959048673643125 64-65 21.674875856575053 27.156332727507117 28.761051535183206 22.407739880734624 66-67 21.635465864141963 27.484620823971483 28.579062278523377 22.300851033363177 68-69 21.87248790119921 27.225725822460017 28.67913053149449 22.222655744846282 70-71 21.753898925186167 27.34651463801355 28.511928655722514 22.387657781077774 72-73 21.862934654116863 27.32367576072892 28.41499943085857 22.39839015429565 74-75 21.793712575934126 27.63109257859682 28.095154012085104 22.480040833383946 76-77 21.741112738681316 27.36708637043368 28.44390623021662 22.44789466066839 78-79 21.777183740543844 27.493940619072614 28.319907153187184 22.40896848719635 80-81 21.693121480305802 27.784935095441256 28.178992023534814 22.34295140071813 82-83 21.913031166828425 27.66759405213014 28.002281392373344 22.417093388668093 84-85 21.715862876734423 27.551292111941073 28.003990827772974 22.72885418355153 86-87 21.853587518286513 27.416894633654753 28.03072959434592 22.698788253712816 88-89 21.714763585734143 27.395982487891978 28.0503848641891 22.838869062184784 90-91 21.553173721175156 27.51387533840319 28.105434803677692 22.827516136743963 92-93 21.63033077373873 27.188187922054574 28.21905791205136 22.96242339215534 94-95 21.405252713930352 27.068894885991696 28.215725159736714 23.310127240341235 96-97 21.66188494011546 26.88067054916316 28.210430291034395 23.247014219686985 98-99 21.345392179300354 26.92976461640007 27.95643305820094 23.76841014609864 100-101 21.824590307019857 25.83743565960942 28.945282160541968 23.39269187282876 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1795.0 1 1251.0 2 928.5 3 1196.5 4 1008.5 5 881.0 6 1636.0 7 3671.0 8 5877.5 9 6369.5 10 5075.5 11 3864.0 12 3731.5 13 4013.5 14 4303.0 15 4537.5 16 4666.0 17 4839.5 18 5152.0 19 5336.5 20 5801.0 21 6740.5 22 7871.5 23 9570.0 24 11675.5 25 14006.0 26 17230.0 27 20921.5 28 23979.0 29 27501.5 30 31767.0 31 36632.0 32 42327.0 33 46539.5 34 50165.5 35 53839.5 36 58215.5 37 62976.0 38 65685.5 39 66906.5 40 68165.0 41 72583.0 42 77543.0 43 81100.0 44 83997.0 45 87278.5 46 88345.5 47 89836.0 48 97337.5 49 98631.0 50 93386.5 51 89034.0 52 84042.5 53 81658.0 54 79552.5 55 72901.5 56 64635.5 57 57732.0 58 52656.0 59 46405.0 60 38740.0 61 32392.5 62 27339.5 63 21254.0 64 15788.0 65 12389.0 66 9223.0 67 7239.5 68 5637.0 69 4001.5 70 3094.0 71 2561.0 72 2193.5 73 1983.0 74 1698.0 75 1470.5 76 1058.0 77 625.5 78 409.5 79 273.5 80 171.0 81 103.0 82 64.5 83 37.0 84 19.5 85 11.5 86 6.0 87 3.5 88 2.0 89 3.0 90 2.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.39743367491204457 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.45217220972882E-4 16-17 0.0018099904231395612 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.033301410465204E-5 26-27 0.003519425822771369 28-29 4.022200940310135E-5 30-31 0.0 32-33 6.033301410465204E-5 34-35 0.011342606651674582 36-37 0.02535997692865541 38-39 0.01892445542415919 40-41 0.00830584494174043 42-43 0.008426510969949735 44-45 0.0034792038133682674 46-47 0.004947307156581467 48-49 0.0027753186488139936 50-51 6.033301410465203E-4 52-53 0.0 54-55 0.0 56-57 8.04440188062027E-5 58-59 0.0015887693714225037 60-61 0.002252432526573676 62-63 0.0012066602820930406 64-65 0.0025943196065000376 66-67 0.001890434441945764 68-69 0.004323866010833397 70-71 1.2066602820930407E-4 72-73 0.0 74-75 0.0 76-77 0.09116318431212923 78-79 0.015525695629597125 80-81 4.826641128372163E-4 82-83 0.0 84-85 0.0 86-87 0.099408696239765 88-89 0.3918830376144165 90-91 0.39679012276159487 92-93 0.0 94-95 2.0111004701550677E-5 96-97 0.0 98-99 1.608880376124054E-4 100-101 4.0222009403101356E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2486201.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.96088940313875 #Duplication Level Percentage of deduplicated Percentage of total 1 72.86250444992767 35.674130220086134 2 14.548601130502604 14.246249018418347 3 5.232718481095174 7.685956524919829 4 2.4525748678812223 4.8032098743700065 5 1.3133520781642685 3.2151442923191595 6 0.7941790810647543 2.3330228492578686 7 0.5190132553464484 1.778794541564633 8 0.3790412924453397 1.484655903891124 9 0.25887189713012376 1.140713849247183 >10 1.4354846212516783 12.82100527326584 >50 0.12377115383983006 4.1722981774026175 >100 0.0732308958366503 6.547569655582746 >500 0.004766594318993564 1.5663197750155782 >1k 0.0016436532134451551 1.1413327617925357 >5k 8.218266067225775E-5 0.3315960969480799 >10k+ 1.643653213445155E-4 1.0580011859183125 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14582 0.586517341116024 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11712 0.4710801741291231 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8241 0.3314695794909583 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3065 0.12328045882050566 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.022200940310136E-5 0.0 3 0.0 0.0 0.0 4.022200940310136E-5 0.0 4 0.0 0.0 0.0 8.044401880620272E-5 0.0 5 0.0 0.0 0.0 8.044401880620272E-5 0.0 6 0.0 0.0 0.0 8.044401880620272E-5 0.0 7 0.0 0.0 0.0 8.044401880620272E-5 0.0 8 0.0 0.0 0.0 8.044401880620272E-5 0.0 9 0.0 0.0 0.0 8.044401880620272E-5 0.0 10-11 0.0 0.0 0.0 8.044401880620272E-5 0.0 12-13 0.0 0.0 0.0 1.6088803761240544E-4 0.0 14-15 0.0 0.0 0.0 1.809990423139561E-4 0.0 16-17 0.0 0.0 0.0 3.016650705232602E-4 0.0 18-19 0.0 0.0 0.0 4.826641128372163E-4 0.0 20-21 0.0 0.0 0.0 5.832191363449697E-4 0.0 22-23 0.0 0.0 0.0 7.038851645542738E-4 0.0 24-25 0.0 0.0 0.0 9.251062162713312E-4 0.0 26-27 0.0 0.0 0.0 0.0013273263103023448 0.0 28-29 2.011100470155068E-5 0.0 0.0 0.0030367617099341525 0.0 30-31 4.022200940310136E-5 0.0 0.0 0.007260072697259796 0.0 32-33 6.033301410465204E-5 0.0 0.0 0.015244141563775414 0.0 34-35 8.044401880620272E-5 0.0 0.0 0.02908051279844228 0.0 36-37 8.044401880620272E-5 0.0 0.0 0.049895402664547235 0.0 38-39 8.044401880620272E-5 0.0 0.0 0.08386288960546634 0.0 40-41 8.044401880620272E-5 0.0 0.0 0.12082691624691648 0.0 42-43 8.044401880620272E-5 0.0 0.0 0.16173269980987054 0.0 44-45 8.044401880620272E-5 0.0 0.0 0.21124599338508832 0.0 46-47 1.0055502350775339E-4 0.0 0.0 0.2740928830774342 0.0 48-49 1.2066602820930407E-4 0.0 0.0 0.3421083009780786 0.0 50-51 1.2066602820930407E-4 2.011100470155068E-5 0.0 0.41545313512463394 0.0 52-53 1.2066602820930407E-4 4.022200940310136E-5 0.0 0.49082918074604587 0.0 54-55 1.2066602820930407E-4 4.022200940310136E-5 0.0 0.5796795995174968 0.0 56-57 1.6088803761240544E-4 4.022200940310136E-5 0.0 0.6853428182194441 0.0 58-59 1.6088803761240544E-4 4.022200940310136E-5 0.0 0.8024894206059767 0.0 60-61 1.6088803761240544E-4 4.022200940310136E-5 0.0 0.9362276018712887 0.0 62-63 1.6088803761240544E-4 4.022200940310136E-5 0.0 1.0734449869499691 0.0 64-65 1.6088803761240544E-4 4.022200940310136E-5 0.0 1.2225881978166688 0.0 66-67 1.6088803761240544E-4 4.022200940310136E-5 0.0 1.40183758272159 0.0 68-69 1.6088803761240544E-4 4.022200940310136E-5 0.0 1.601419193379779 0.0 70-71 1.6088803761240544E-4 4.022200940310136E-5 0.0 1.8222782470122088 0.0 72-73 1.809990423139561E-4 4.022200940310136E-5 0.0 2.0516643666380956 0.0 74-75 2.0111004701550678E-4 4.022200940310136E-5 0.0 2.295510298644398 0.0 76-77 2.0111004701550678E-4 4.022200940310136E-5 0.0 2.5695428487077274 0.0 78-79 2.0111004701550678E-4 4.022200940310136E-5 0.0 2.8795338751774295 0.0 80-81 2.2122105171705746E-4 4.022200940310136E-5 0.0 3.2174590871775854 0.0 82-83 2.4133205641860815E-4 4.022200940310136E-5 0.0 3.564394029284036 0.0 84-85 2.4133205641860815E-4 4.022200940310136E-5 0.0 3.9225710230186537 0.0 86-87 2.4133205641860815E-4 4.022200940310136E-5 0.0 4.3085213142461125 0.0 88-89 2.4133205641860815E-4 4.022200940310136E-5 0.0 4.718906476185956 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7940 0.0 41.706852 1 GTATCAA 13370 0.0 37.811775 1 ATCAACG 17170 0.0 29.1568 3 TCAACGC 17385 0.0 28.85081 4 TATCAAC 17645 0.0 28.479475 2 CAACGCA 17760 0.0 28.214909 5 AACGCAG 18165 0.0 27.559717 6 ACGCAGA 20570 0.0 24.42977 7 CGCAGAG 20580 0.0 24.325668 8 GCAGAGT 22050 0.0 22.576197 9 GTACATG 11970 0.0 22.410799 1 TACATGG 11990 0.0 21.925438 2 GAGTACT 12370 0.0 21.867432 12-13 AGTACTT 12670 0.0 21.836576 12-13 ACATGGG 12065 0.0 21.002531 3 AGAGTAC 19690 0.0 20.558714 10-11 GTACTTT 13075 0.0 20.543165 14-15 ATTCGCG 315 0.0 19.605589 80-81 CGTAGAC 195 1.04243045E-5 19.467628 3 TCGCGGA 295 0.0 19.41569 82-83 >>END_MODULE