##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939059_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 604308 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7986440689185 33.0 31.0 34.0 30.0 34.0 2 31.86284808408957 33.0 31.0 34.0 30.0 34.0 3 31.876387537480888 34.0 31.0 34.0 30.0 34.0 4 35.386221926567245 37.0 35.0 37.0 33.0 37.0 5 35.45217174023842 37.0 35.0 37.0 33.0 37.0 6 35.59103304937217 37.0 35.0 37.0 33.0 37.0 7 35.56079019308035 37.0 35.0 37.0 33.0 37.0 8 35.56476995174646 37.0 35.0 37.0 33.0 37.0 9 37.12209998874746 39.0 37.0 39.0 33.0 39.0 10-11 37.031026397135236 39.0 37.0 39.0 33.0 39.0 12-13 36.98149867286219 39.0 37.0 39.0 33.0 39.0 14-15 38.07709313793629 40.0 38.0 41.0 33.0 41.0 16-17 38.00206765424254 40.0 38.0 41.0 33.0 41.0 18-19 37.95289322663278 40.0 37.5 41.0 33.0 41.0 20-21 38.04721764398287 40.0 38.0 41.0 33.0 41.0 22-23 37.97630181960193 40.0 38.0 41.0 33.0 41.0 24-25 37.85381709327032 40.0 37.5 41.0 33.0 41.0 26-27 37.674617082679696 40.0 37.0 41.0 33.0 41.0 28-29 37.59506245159753 40.0 37.0 41.0 33.0 41.0 30-31 37.44538050133376 40.0 36.5 41.0 33.0 41.0 32-33 37.173540644836734 40.0 35.5 41.0 32.0 41.0 34-35 36.81417505642818 39.0 35.0 41.0 31.0 41.0 36-37 36.49642235416377 39.0 35.0 41.0 30.5 41.0 38-39 36.24752278639369 39.0 35.0 41.0 30.0 41.0 40-41 36.23746996564665 39.0 35.0 41.0 30.0 41.0 42-43 36.01633686795475 39.0 35.0 41.0 30.0 41.0 44-45 35.84924823103451 39.0 35.0 41.0 29.5 41.0 46-47 35.57059893299443 39.0 35.0 41.0 27.5 41.0 48-49 35.25738116986702 39.0 35.0 41.0 25.5 41.0 50-51 34.138570894312174 38.0 34.0 40.0 23.5 40.5 52-53 34.3692454840909 38.0 34.5 39.5 24.0 40.5 54-55 34.943878783666605 39.0 35.0 40.5 24.0 41.0 56-57 34.85353577976794 39.0 35.0 41.0 23.5 41.0 58-59 34.7085914798414 39.0 35.0 40.5 23.0 41.0 60-61 34.31011835024523 38.0 34.0 40.0 22.0 41.0 62-63 34.00440421109765 38.0 34.0 40.0 22.0 41.0 64-65 33.638951494933046 37.5 34.0 40.0 20.0 41.0 66-67 33.12829550494119 37.0 33.0 39.0 17.0 41.0 68-69 32.628520555743094 36.0 33.0 39.0 13.0 40.5 70-71 32.02997974542783 36.0 32.0 38.5 8.0 40.0 72-73 31.526756223647546 35.0 31.5 37.5 4.5 40.0 74-75 31.028428384201433 35.0 31.0 37.0 2.0 39.0 76-77 30.594239195906724 35.0 31.0 36.5 2.0 39.0 78-79 30.11192057692435 35.0 30.5 36.0 2.0 38.5 80-81 29.553952620187058 34.0 29.5 36.0 2.0 37.0 82-83 29.010089226023815 34.0 29.0 35.0 2.0 37.0 84-85 28.499029468416765 34.0 29.0 35.0 2.0 36.5 86-87 28.140279460142843 34.0 29.0 35.0 2.0 36.0 88-89 27.548249402622503 34.0 26.5 35.0 2.0 36.0 90-91 26.988207999894094 34.0 25.5 35.0 2.0 35.0 92-93 26.427558463564935 33.0 25.0 35.0 2.0 35.0 94-95 25.817969479139776 33.0 23.5 35.0 2.0 35.0 96-97 25.090222204571177 33.0 17.5 35.0 2.0 35.0 98-99 24.307241505987015 32.0 2.0 35.0 2.0 35.0 100-101 21.211051318201974 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06031937713127178 1101 2 0.12453552142934043 1101 3 0.15709412665652778 1101 4 -0.05618824772609088 1101 5 -0.28474604207629284 1101 6 -0.019852425325353806 1101 7 0.04490823242856834 1101 8 0.0355977662414233 1101 9 0.2118766922176789 1101 10-11 0.19772028583936674 1101 12-13 0.1840032412222854 1101 14-15 0.14113482767321273 1101 16-17 0.01960492241498457 1101 18-19 0.2551931580943858 1101 20-21 0.19027815952768634 1101 22-23 0.2658524682410146 1101 24-25 0.24499799402967426 1101 26-27 0.19626975111953726 1101 28-29 0.27783354532746074 1101 30-31 0.3357898691491954 1101 32-33 0.1433630470846623 1101 34-35 0.15885800940981198 1101 36-37 0.4097441379448057 1101 38-39 0.4040340255840178 1101 40-41 0.3712265930250922 1101 42-43 0.43444764553631643 1101 44-45 0.23708571561147807 1101 46-47 0.3302693264007459 1101 48-49 0.1277009116533705 1101 50-51 -0.027686888145716182 1101 52-53 0.22426908769673304 1101 54-55 0.4405122654749576 1101 56-57 0.3745265723331812 1101 58-59 0.4326215649452365 1101 60-61 0.42788868184715056 1101 62-63 0.162084896183039 1101 64-65 0.019013744297929236 1101 66-67 -0.1542951768474765 1101 68-69 -0.25617642234411164 1101 70-71 -0.3143195490856172 1101 72-73 -0.35058099674622056 1101 74-75 -0.43930136670039843 1101 76-77 -0.22494371824735282 1101 78-79 -0.4456479378728666 1101 80-81 -0.6227958888124938 1101 82-83 -0.5839843320725748 1101 84-85 -0.5511582496499621 1101 86-87 -0.7739718627453804 1101 88-89 -0.6670580320703721 1101 90-91 -0.5394337731063708 1101 92-93 -0.5293429727910741 1101 94-95 -1.0344677686159187 1101 96-97 -0.747545200369462 1101 98-99 -0.778841896698971 1101 100-101 -0.1120892438932799 1102 1 0.38570919157387706 1102 2 0.5299306219917277 1102 3 0.679970878157814 1102 4 0.5271076385437752 1102 5 0.46877057761133045 1102 6 0.6038590552634489 1102 7 0.5686292449971617 1102 8 0.6859034704504765 1102 9 0.7001591656302679 1102 10-11 0.7282668767948834 1102 12-13 0.7573314000523084 1102 14-15 1.0468465261358375 1102 16-17 0.891883201756464 1102 18-19 0.8647360315777846 1102 20-21 0.8668853194062223 1102 22-23 1.040220293724083 1102 24-25 1.0216432818273802 1102 26-27 1.1424549166355575 1102 28-29 0.912666803766669 1102 30-31 1.1598499478561166 1102 32-33 1.2498931340980235 1102 34-35 1.2416126153986227 1102 36-37 1.167236954917179 1102 38-39 1.3090648683471997 1102 40-41 1.3830428436889193 1102 42-43 1.559095302711853 1102 44-45 1.6535617426823066 1102 46-47 2.0019607081293103 1102 48-49 1.8889690734791529 1102 50-51 1.6627586638240004 1102 52-53 1.6765921673432302 1102 54-55 1.9539115056400007 1102 56-57 1.9821017728451835 1102 58-59 1.8095262587644285 1102 60-61 1.9774719981590962 1102 62-63 2.0244328951481307 1102 64-65 2.1369243343274746 1102 66-67 1.9641022250071742 1102 68-69 1.9673416952260467 1102 70-71 1.7281181866606659 1102 72-73 1.9317732660913087 1102 74-75 2.003510158824877 1102 76-77 1.7823073949517187 1102 78-79 1.7497712649812804 1102 80-81 1.7318584407488977 1102 82-83 1.7967467707774851 1102 84-85 1.9708640279485259 1102 86-87 1.9580092911688567 1102 88-89 1.8595600214993553 1102 90-91 1.7914382668734774 1102 92-93 1.7432001594174835 1102 94-95 1.6108540718732058 1102 96-97 1.3591074406697992 1102 98-99 1.2143086949646325 1102 100-101 1.5862440213544815 1103 1 0.19970402354548966 1103 2 0.2151632844709006 1103 3 0.34730772431113976 1103 4 0.3271906238560476 1103 5 0.1601360570707797 1103 6 0.2998427010848346 1103 7 0.13025089944092372 1103 8 0.1026752743352688 1103 9 0.41571520095637027 1103 10-11 0.3080491584186902 1103 12-13 0.16965370690905246 1103 14-15 0.373243614228457 1103 16-17 0.3087545205482485 1103 18-19 0.3110755696936778 1103 20-21 0.12900927112631422 1103 22-23 0.042043609223448186 1103 24-25 0.5063011192166442 1103 26-27 0.49499069140127716 1103 28-29 0.25260635267740383 1103 30-31 0.352186962678779 1103 32-33 0.312585838811998 1103 34-35 0.5326987784411443 1103 36-37 0.6806933943750053 1103 38-39 0.6111499586527671 1103 40-41 0.5399458048477541 1103 42-43 0.5093564028407158 1103 44-45 0.6674803361149984 1103 46-47 0.6894231792025138 1103 48-49 1.0262237439330093 1103 50-51 0.631841574921765 1103 52-53 0.8022525077854112 1103 54-55 0.7662736184595431 1103 56-57 0.8678620330007618 1103 58-59 0.8776366224829815 1103 60-61 1.211264561865498 1103 62-63 0.9898489349271102 1103 64-65 1.0282742962082025 1103 66-67 1.0614514177557481 1103 68-69 1.1911321674327198 1103 70-71 1.284328928873208 1103 72-73 1.3383861159958492 1103 74-75 1.11159178128894 1103 76-77 1.286284617844867 1103 78-79 1.0739351710931793 1103 80-81 1.0603891959152385 1103 82-83 0.917679309790703 1103 84-85 0.9268953059897989 1103 86-87 0.861683921277006 1103 88-89 1.0874559052929307 1103 90-91 1.2651157306264373 1103 92-93 1.1485969034246644 1103 94-95 0.7833825601237017 1103 96-97 0.6638895536242764 1103 98-99 0.6536501424173871 1103 100-101 0.7074396845285733 1104 1 0.1960514921428249 1104 2 0.19056805520380848 1104 3 0.24049638747814228 1104 4 0.09216340062555872 1104 5 0.044845513321718045 1104 6 1.5623984459978146E-4 1104 7 0.08945508342703334 1104 8 -0.006152598089585126 1104 9 0.19746100038263137 1104 10-11 0.2570373128983263 1104 12-13 0.1066272122333558 1104 14-15 0.2654012422622074 1104 16-17 0.36202683741754527 1104 18-19 0.34587219232181354 1104 20-21 0.19203088708686522 1104 22-23 0.3560588568345011 1104 24-25 0.38791421759106015 1104 26-27 0.38969013002378716 1104 28-29 0.3512019332119749 1104 30-31 0.3671223359655329 1104 32-33 0.432395699020546 1104 34-35 0.7018096813937404 1104 36-37 0.8500726266603849 1104 38-39 0.613514509258053 1104 40-41 0.8017181444843828 1104 42-43 0.7501453886534151 1104 44-45 0.7687234327330685 1104 46-47 0.8256905718220011 1104 48-49 0.8515478532422449 1104 50-51 0.8962565519267542 1104 52-53 0.9146369635484817 1104 54-55 0.9202377371359205 1104 56-57 0.791528029657222 1104 58-59 0.8261482891171283 1104 60-61 0.9883479358547973 1104 62-63 1.0641192417657663 1104 64-65 0.9667667081799252 1104 66-67 1.099653383552699 1104 68-69 1.0074810671198264 1104 70-71 1.0239470230519174 1104 72-73 0.9951002037736849 1104 74-75 0.873245405523793 1104 76-77 1.0611644388285448 1104 78-79 0.8416221397197887 1104 80-81 1.1416198030225502 1104 82-83 1.1122522270672057 1104 84-85 1.0240711804181792 1104 86-87 0.7767292282782918 1104 88-89 0.8880839585554909 1104 90-91 0.825684979109159 1104 92-93 0.7480901675452891 1104 94-95 0.8324234047268675 1104 96-97 1.264428489023583 1104 98-99 1.2678023193000847 1104 100-101 1.6306438294849528 1105 1 0.07610869251597663 1105 2 0.12370130997869566 1105 3 0.09637231422238557 1105 4 -0.03797358567581455 1105 5 0.16454895516390877 1105 6 -0.027805659305990105 1105 7 0.009601644039790358 1105 8 0.22890902119651457 1105 9 0.09748309494737128 1105 10-11 0.10835930435509766 1105 12-13 0.269807847574846 1105 14-15 0.2799683115409408 1105 16-17 0.2775550202623478 1105 18-19 0.3148047793498492 1105 20-21 0.15077542481947148 1105 22-23 -0.03641119275552285 1105 24-25 0.34779417045598393 1105 26-27 0.09442695920825628 1105 28-29 0.23855033994836816 1105 30-31 0.2302495761089034 1105 32-33 0.3687694899568612 1105 34-35 0.485734044333789 1105 36-37 0.2834206897035543 1105 38-39 0.34464820592422285 1105 40-41 0.632234420813738 1105 42-43 0.7744954402073958 1105 44-45 0.8763331690712661 1105 46-47 0.877345822963548 1105 48-49 0.8185471658557901 1105 50-51 0.8377949473703623 1105 52-53 0.9022885568120884 1105 54-55 1.132735282184278 1105 56-57 1.0895806392708067 1105 58-59 0.9813288508100584 1105 60-61 1.1721777831568048 1105 62-63 1.1015449794787386 1105 64-65 1.205964920975127 1105 66-67 1.1762877676053378 1105 68-69 1.4185657875008317 1105 70-71 1.3839687493739135 1105 72-73 1.2073277041419281 1105 74-75 1.2328221718614962 1105 76-77 1.2180696040467929 1105 78-79 1.071657245528204 1105 80-81 1.3203648317454864 1105 82-83 1.2570544807128137 1105 84-85 1.0979805681532397 1105 86-87 1.235740742001468 1105 88-89 0.9350011530367581 1105 90-91 1.2513735930434642 1105 92-93 1.3082272765942413 1105 94-95 1.5496590064355118 1105 96-97 1.3668797582335799 1105 98-99 1.4822945125538745 1105 100-101 1.7186630517562769 1106 1 0.24065113002480487 1106 2 0.12030607390092385 1106 3 0.07052262255924902 1106 4 0.014843812996694794 1106 5 0.12889014991041847 1106 6 0.29714844322190004 1106 7 0.20740209595336268 1106 8 0.08561475955738729 1106 9 0.09824805281584759 1106 10-11 0.21455310937322025 1106 12-13 0.08086325406168271 1106 14-15 0.3885952710099403 1106 16-17 0.36361572261134967 1106 18-19 0.3459091431402683 1106 20-21 0.3473813655999649 1106 22-23 0.4694701563947845 1106 24-25 0.39489792228011567 1106 26-27 0.39435517085444616 1106 28-29 0.33250481907089835 1106 30-31 0.37780112250065656 1106 32-33 0.556319506432871 1106 34-35 0.4980351552879867 1106 36-37 0.6126820934600801 1106 38-39 0.7926428394505649 1106 40-41 0.7331559008306883 1106 42-43 0.473069676426384 1106 44-45 0.538566022246421 1106 46-47 0.6049001938151193 1106 48-49 0.4794531113437088 1106 50-51 0.3394631439527629 1106 52-53 0.4384887168648177 1106 54-55 0.41999755681592177 1106 56-57 0.2698194238115974 1106 58-59 0.1495695653983944 1106 60-61 0.4041924468107112 1106 62-63 0.18013557402752411 1106 64-65 0.32492175381418775 1106 66-67 0.4275087580492496 1106 68-69 0.2985703772480477 1106 70-71 0.45330629585981796 1106 72-73 0.19352609890726669 1106 74-75 0.4003950193877408 1106 76-77 0.07393094660669774 1106 78-79 0.5644725258558445 1106 80-81 0.23997364406013233 1106 82-83 0.12391944799615118 1106 84-85 0.24082468150822578 1106 86-87 0.48440206573163636 1106 88-89 0.4425771780803025 1106 90-91 0.5494129471836509 1106 92-93 0.3236176404788189 1106 94-95 0.20208863031161428 1106 96-97 0.3410182979878513 1106 98-99 0.7651141472570728 1106 100-101 1.1556687712874165 1107 1 0.10396976927031076 1107 2 0.25311194216930843 1107 3 0.06922884032445964 1107 4 0.35195897132402365 1107 5 0.38623573026834634 1107 6 0.345814559592057 1107 7 -0.022220833848706434 1107 8 0.15496675613852773 1107 9 0.299071155902773 1107 10-11 0.21746119229236882 1107 12-13 0.45668796705863457 1107 14-15 0.3922658658570555 1107 16-17 0.25220409496305507 1107 18-19 0.36266830645539727 1107 20-21 0.43078055888868505 1107 22-23 0.3326102419834527 1107 24-25 0.35184600121839793 1107 26-27 0.3502407091790829 1107 28-29 0.20869981137260396 1107 30-31 0.26165928650073056 1107 32-33 0.28234501911603616 1107 34-35 0.27280581628777156 1107 36-37 0.32014095294128 1107 38-39 0.5365573795724501 1107 40-41 0.2819712997663899 1107 42-43 0.33692251308784193 1107 44-45 0.18531224773920485 1107 46-47 0.4848331044738998 1107 48-49 0.6639970970125901 1107 50-51 0.6019238475127153 1107 52-53 0.6861850819807032 1107 54-55 0.7881356812414779 1107 56-57 0.6664997893489897 1107 58-59 0.6080338868652291 1107 60-61 0.7751610857635072 1107 62-63 0.6758898022366608 1107 64-65 0.6606509259691791 1107 66-67 0.6386967545601365 1107 68-69 0.6336526582856479 1107 70-71 0.5265894000005034 1107 72-73 0.7470190182302865 1107 74-75 0.42823768141786545 1107 76-77 0.5776659378546363 1107 78-79 0.6670955083458772 1107 80-81 0.9275735316591387 1107 82-83 1.0470491716185073 1107 84-85 1.074636597184071 1107 86-87 0.8804062690603445 1107 88-89 0.898606144752236 1107 90-91 0.8924496472779033 1107 92-93 1.1145547251952088 1107 94-95 1.2940013205005272 1107 96-97 1.322718011716578 1107 98-99 1.297624084792563 1107 100-101 1.2735932167465904 1108 1 0.11624519305032166 1108 2 0.04256607243861765 1108 3 0.16473187897512886 1108 4 0.11521304601563287 1108 5 0.29658043286720925 1108 6 0.25984128521941585 1108 7 0.4177552435734526 1108 8 0.3034256830055142 1108 9 0.3447385112497088 1108 10-11 0.3087989235137485 1108 12-13 0.4636774677050255 1108 14-15 0.4354805528700041 1108 16-17 0.6399190054950239 1108 18-19 0.21822215037870052 1108 20-21 0.1636239626607221 1108 22-23 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2710.0 13 3938.0 14 3545.0 15 4050.0 16 5211.0 17 7048.0 18 5847.0 19 3971.0 20 3997.0 21 4122.0 22 4645.0 23 5188.0 24 5871.0 25 6816.0 26 7896.0 27 9236.0 28 10930.0 29 12519.0 30 15414.0 31 18088.0 32 21827.0 33 27441.0 34 37053.0 35 57410.0 36 71887.0 37 112260.0 38 109377.0 39 15121.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.19873402801872 23.45451315857187 16.636425662742806 17.7103271506666 2 13.858514722591202 26.282698926405264 37.40273549686468 22.456050854138855 3 21.720282986832927 28.235241501201752 33.57876306726983 16.465712444695487 4 10.816926606644413 19.913693853378945 43.7910099144393 25.478369625537344 5 9.260244105694088 41.71202022561636 37.174841904534695 11.852893764154866 6 22.988530488501514 41.7623011214523 18.388307622001197 16.860860768044983 7 21.08079736001576 37.04207185115151 25.609701577797907 16.267429211034816 8 21.638161849079285 31.256281739861873 22.793091286616228 24.31246512444261 9 20.95761186516735 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17.848567246038897 34-35 20.459234912809276 28.916859639949205 32.36871591842833 18.255189528813194 36-37 19.101368350513233 28.237839240015898 34.98855135097384 17.672241058497026 38-39 17.820967428220897 30.975274839451867 33.63880285869603 17.564954873631205 40-41 17.2138511287056 31.3639125025659 33.50131847839366 17.92091789033484 42-43 16.74352061755828 31.427155838531057 30.76327736171069 21.066046182199976 44-45 18.44423050383015 28.829190589495074 31.699929998187603 21.026648908487182 46-47 20.676207617519545 31.20855277104628 31.635692522560344 16.479547088873833 48-49 19.002172660398962 29.994187946349506 32.31742959224993 18.686209801001603 50-51 15.169335735647849 31.117151078921353 35.1790802416837 18.534432943747095 52-53 17.833639720402054 32.99144361106053 30.45462519757185 18.720291470965563 54-55 16.65854700783644 29.72052854822163 32.08825431769093 21.532670126251 56-57 19.338319391432712 29.39518971180828 30.9816904499089 20.284800446850106 58-59 21.557901910187528 28.772069792276977 31.003045828710572 18.666982468824926 60-61 18.59406060676545 30.595874872174868 33.535250661866286 17.2748138591934 62-63 17.186101087083927 30.356134912460526 33.37498119196005 19.082782808495498 64-65 17.106492875863076 32.729206300637244 31.534283661713253 18.630017161786423 66-67 18.438559202232575 29.698159586746897 29.6333808986858 22.229900312334728 68-69 16.958518140256707 29.535064176829138 32.015392791469594 21.49102489144456 70-71 20.486192256352894 29.844519941284343 30.28962787080892 19.379659931553846 72-73 19.27296162321733 27.204440563801917 34.090973348301524 19.431624464679224 74-75 17.031685406152473 32.80121010427087 30.75088500101036 19.416219488566295 76-77 18.253745240510117 29.190762017192355 30.954890843489686 21.60060189880784 78-79 18.607115783594 29.30439740929886 32.131576497499736 19.956910309607405 80-81 20.347661860935258 28.74835412565835 30.722655210937916 20.181328802468478 82-83 18.798096946651825 28.264897843477772 33.234600456061095 19.702404753809308 84-85 18.304272861947936 29.29243973284429 31.982369186312344 20.42091821889543 86-87 18.313100167679085 30.043736225915357 31.33861449023313 20.304549116172428 88-89 18.612469945827666 29.46015556671444 29.85489197125441 22.072482516203483 90-91 19.44296855057244 27.073381443316112 31.2110395198647 22.272610486246748 92-93 21.0262410162126 29.117990192776716 31.194686770060777 18.661082020949905 94-95 19.88450670024133 27.534191438793044 31.728224839375496 20.853077021590128 96-97 17.79288587244396 27.805916049735863 33.15369372680533 21.247504351014847 98-99 19.68282426275476 28.993115468699454 29.88314009135579 21.44092017718999 100-101 19.31853987557666 26.337409556969444 31.593435143316302 22.750615424137596 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11159.0 1 6431.0 2 1647.5 3 1397.0 4 1033.0 5 1044.5 6 1698.0 7 2878.0 8 3828.0 9 3801.0 10 2806.0 11 1953.0 12 1805.0 13 1672.0 14 1506.5 15 1422.0 16 1422.0 17 1403.5 18 1269.0 19 1269.0 20 1422.0 21 1617.0 22 1853.5 23 2051.0 24 2209.5 25 2734.0 26 4205.5 27 5726.0 28 5888.5 29 6276.5 30 8035.5 31 9900.5 32 12616.0 33 16790.5 34 20901.0 35 23368.0 36 26364.5 37 29586.5 38 28119.5 39 24711.0 40 22476.0 41 20345.5 42 17943.0 43 16556.0 44 16062.0 45 15711.0 46 18385.0 47 30845.0 48 55831.5 49 54234.0 50 26815.0 51 11381.0 52 5308.5 53 3179.0 54 2141.0 55 1499.0 56 999.0 57 656.5 58 490.0 59 433.5 60 410.0 61 368.5 62 316.5 63 253.0 64 211.5 65 188.0 66 161.5 67 161.0 68 137.0 69 108.0 70 86.5 71 65.5 72 63.0 73 52.0 74 45.5 75 42.0 76 26.5 77 16.5 78 15.5 79 13.0 80 7.5 81 5.0 82 5.5 83 4.0 84 3.5 85 3.0 86 1.0 87 0.5 88 0.5 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3185461718196681 2 0.08968936370195331 3 0.10011451114332426 4 0.10640269531430992 5 0.11864810659465042 6 0.05940679256273291 7 0.060399663747625384 8 0.062054449055779504 9 0.06437114848719527 10-11 0.062302666852002614 12-13 0.06114431713629474 14-15 0.0591585747665098 16-17 0.06106157787088703 18-19 0.0675979798380958 20-21 0.06917002588084223 22-23 0.08414583291963701 24-25 0.09283345578744613 26-27 0.08654527161646047 28-29 0.08571787896238342 30-31 0.07967791258762089 32-33 0.07214863943551963 34-35 0.31325085883357495 36-37 0.4843556596967109 38-39 0.4592029230127683 40-41 0.44141398095011153 42-43 0.47037272384280865 44-45 0.47906034671061776 46-47 0.47417873005156314 48-49 0.4917194543179968 50-51 0.48708605545516526 52-53 0.4872515339859807 54-55 0.47773651846409443 56-57 0.45738265917379883 58-59 0.4410830238884807 60-61 0.399051477061366 62-63 0.46937985265791615 64-65 0.4918021935834045 66-67 0.5013999483706985 68-69 0.5158793198170469 70-71 0.5138935774472619 72-73 0.5018963839631446 74-75 0.502641037351814 76-77 0.47624721168675577 78-79 0.4587064874203221 80-81 0.46400180040641525 82-83 0.47219298768177814 84-85 0.4734340766628937 86-87 0.4735168159283014 88-89 0.47889486817980237 90-91 0.49271232550288924 92-93 0.4991659882046903 94-95 0.5065297828259762 96-97 0.4983385955506132 98-99 0.5018136446977369 100-101 0.4788121289143947 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 604308.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.583803770425394 #Duplication Level Percentage of deduplicated Percentage of total 1 73.4309925856493 12.1776517170797 2 9.187999072284809 3.0474394731524366 3 3.7150096381451205 1.8482697253271316 4 2.2129998595440923 1.4679982165863275 5 1.4989998494275658 1.2429559677401982 6 1.0289998961090205 1.0238839414116068 7 0.8049999187189831 0.9344972481070808 8 0.6659999327537757 0.88358497567241 9 0.5629999431537166 0.8403012522019698 >10 5.214999473439843 18.701387785585936 >50 0.9529999037752963 11.063054378210659 >100 0.6649999328547451 19.982486497498353 >500 0.0249999974757423 2.706974016122509 >1k 0.021999997778653222 9.45376222281932 >5k 0.008999999091267228 9.496714270247839 >10k+ 0.001999999798059384 5.129038312236535 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 19066 3.1550136685266454 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11862 1.9629063325324172 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8583 1.4203022299886812 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8303 1.373968241360366 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8101 1.3405415781356527 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5986 0.9905544854610563 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5558 0.9197296742720599 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5382 0.8906054528485474 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5158 0.8535382619458952 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5142 0.8508906054528486 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5052 0.8359975376794615 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4829 0.7990958253076245 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4629 0.7660001191445421 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4368 0.7228102226017197 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4194 0.694016958239838 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3530 0.5841392137784044 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3339 0.5525328143926607 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3289 0.54425888785189 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2961 0.48998192974443494 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2851 0.47177929135473967 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2834 0.4689661563308777 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2779 0.45986483713602994 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2283 0.3777874858515856 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1941 0.3211938283127147 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1928 0.3190426074121143 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1792 0.2965375272212183 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1727 0.2857814227182165 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1586 0.26244894987324346 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1469 0.24308796176784023 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1280 0.21181251944372737 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1253 0.20734459911171127 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1132 0.1873216968830464 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1012 0.16746427318519694 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 997 0.16498209522296578 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 970 0.16051417489094966 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 909 0.15041998451120953 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 823 0.13618883086108408 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 788 0.13039708228254468 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 740 0.12245411280340489 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 722 0.11947549924872748 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 711 0.11765523540975793 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 699 0.115669493039973 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 636 0.10524434559860203 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 621 0.10276216763637086 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 617 0.1021002535131092 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.4821779622311805E-4 0.0 12-13 0.0 0.0 0.0 6.619141232616481E-4 0.0 14-15 0.0 0.0 0.0 9.928711848924722E-4 0.0 16-17 0.0 0.0 0.0 0.0013238282465232961 0.0 18-19 0.0 0.0 0.0 0.0017375245735618261 0.0 20-21 0.0 0.0 0.0 0.002978613554677416 0.0 22-23 0.0 0.0 0.0 0.005212573720685478 0.0 24-25 0.0 0.0 0.0 0.01034240817596325 0.0 26-27 0.0 0.0 0.0 0.017044288673987436 0.0 28-29 0.0 0.0 0.0 0.04451372478934583 0.0 30-31 0.0 0.0 0.0 0.09870794363139326 0.0 32-33 0.0 0.0 0.0 0.20304215731051053 0.0 34-35 0.0 0.0 0.0 0.3340184144509092 0.0 36-37 0.0 0.0 0.0 0.5182787585138704 0.0 38-39 0.0 0.0 0.0 0.7655864228175037 0.0 40-41 0.0 0.0 0.0 1.0334961642076557 0.0 42-43 0.0 0.0 0.0 1.3005785129437308 0.0 44-45 0.0 0.0 0.0 1.6115954116112976 0.0 46-47 0.0 0.0 0.0 1.9941817748565303 0.0 48-49 0.0 0.0 0.0 2.313307121534052 0.0 50-51 0.0 0.0 0.0 2.662384082289164 0.0 52-53 0.0 0.0 0.0 2.9866392634219636 0.0 54-55 0.0 0.0 0.0 3.354415298159217 0.0 56-57 0.0 0.0 0.0 3.8789822408440733 0.0 58-59 0.0 0.0 0.0 4.346790047459242 0.0 60-61 0.0 0.0 0.0 4.866475373485043 0.0 62-63 0.0 0.0 0.0 5.358112088537633 0.0 64-65 0.0 0.0 0.0 5.885078469919312 0.0 66-67 0.0 0.0 0.0 6.5082706169701545 0.0 68-69 0.0 0.0 0.0 7.218090774902864 0.0 70-71 0.0 0.0 0.0 7.973086571748182 0.0 72-73 0.0 0.0 0.0 8.637482872972061 0.0 74-75 0.0 0.0 0.0 9.448741370294618 0.0 76-77 0.0 0.0 0.0 10.184872614626979 0.0 78-79 0.0 0.0 0.0 11.217706864711372 0.0 80-81 0.0 0.0 0.0 12.259145998398168 0.0 82-83 0.0 0.0 0.0 13.139988217928606 0.0 84-85 0.0 0.0 0.0 14.118876466967176 0.0 86-87 0.0 0.0 0.0 15.108603559774156 0.0 88-89 0.0 0.0 0.0 16.171223945405323 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCCGA 25 0.0016053338 38.050518 88-89 CATATAG 130 2.1100277E-10 36.438484 3 GTAAGCG 125 0.0 36.147995 94-95 ATACCTT 225 0.0 35.76994 6 GCTTTGC 535 0.0 33.715847 1 CTTTGCG 555 0.0 33.28705 2 AAGCTGC 30 0.003922214 31.714043 66-67 CGGCGGT 30 0.0039254106 31.708767 94-95 CAGTCCA 30 0.00392621 31.707449 84-85 TACAATC 60 0.004014185 31.561712 7 GACATAT 170 1.09139364E-10 30.714825 1 TTGCGTT 620 0.0 29.797277 4 ATAACGC 80 4.5637824E-4 29.606268 3 CGCAGAA 210 1.8189894E-12 29.307302 9 GTCTTCT 65 0.005937442 29.133886 8 TTTGCGT 635 0.0 29.093403 3 CTTACAT 35 0.008337513 27.178946 92-93 AGGGTCT 70 0.008501325 27.068588 5 AATACCT 310 0.0 25.977114 5 TAATACC 350 0.0 25.715158 4 >>END_MODULE