##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939059_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 604308 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0454172375676 33.0 31.0 34.0 30.0 34.0 2 32.28068302918379 34.0 31.0 34.0 30.0 34.0 3 32.35256855775532 34.0 31.0 34.0 30.0 34.0 4 35.903903307584876 37.0 35.0 37.0 35.0 37.0 5 35.8400633451816 37.0 35.0 37.0 35.0 37.0 6 35.92269835911489 37.0 35.0 37.0 35.0 37.0 7 35.87404601626985 37.0 35.0 37.0 35.0 37.0 8 35.88338893411969 37.0 35.0 37.0 35.0 37.0 9 37.486280175010094 39.0 37.0 39.0 34.0 39.0 10-11 37.40419703197707 39.0 37.0 39.0 34.0 39.0 12-13 37.36652170747367 39.0 37.0 39.0 34.0 39.0 14-15 38.50439510977846 40.0 38.0 41.0 34.0 41.0 16-17 38.60452699616752 40.0 38.0 41.0 34.0 41.0 18-19 38.637550719169695 40.0 38.0 41.0 34.0 41.0 20-21 38.60448314435685 40.0 38.0 41.0 34.0 41.0 22-23 38.51720728502684 40.0 38.0 41.0 34.0 41.0 24-25 38.38066929446573 40.0 38.0 41.0 34.0 41.0 26-27 38.14855255929096 40.0 37.0 41.0 33.5 41.0 28-29 38.01015128047287 40.0 37.0 41.0 33.0 41.0 30-31 37.8487600693686 40.0 37.0 41.0 33.0 41.0 32-33 37.63217349431085 40.0 36.0 41.0 33.0 41.0 34-35 37.44509256869014 40.0 36.0 41.0 33.0 41.0 36-37 37.24520443217697 40.0 35.5 41.0 32.0 41.0 38-39 37.06920560376497 39.0 35.0 41.0 31.5 41.0 40-41 37.03202009571278 40.0 35.0 41.0 31.0 41.0 42-43 36.7966244034499 39.5 35.0 41.0 31.0 41.0 44-45 36.534161387901534 39.0 35.0 41.0 30.5 41.0 46-47 36.194408314965216 39.0 35.0 41.0 30.0 41.0 48-49 35.80849417846529 39.0 35.0 41.0 28.0 41.0 50-51 35.55377969512236 39.0 35.0 40.0 27.5 41.0 52-53 35.42905769905413 39.0 35.0 40.0 27.0 41.0 54-55 35.21459752311735 38.5 35.0 40.0 26.5 41.0 56-57 35.07211554372935 38.5 35.0 40.0 26.5 41.0 58-59 34.8407550454404 38.0 34.5 40.0 26.0 41.0 60-61 34.37371754138618 38.0 34.0 40.0 25.0 41.0 62-63 34.028741138624675 37.5 34.0 40.0 23.0 41.0 64-65 33.64509736756753 37.0 33.0 39.5 22.5 41.0 66-67 33.10310470819516 36.5 33.0 39.0 22.0 40.0 68-69 32.53947407613336 36.0 32.0 39.0 20.5 40.0 70-71 31.904376576183004 35.0 31.0 37.5 18.0 40.0 72-73 31.315818258239176 35.0 31.0 37.0 15.0 39.0 74-75 30.79145816371784 34.5 30.0 37.0 9.5 39.0 76-77 28.861766681890693 32.5 28.0 35.0 7.0 36.5 78-79 29.81636930174679 34.0 29.5 35.5 4.5 37.0 80-81 29.72239238931141 34.0 29.5 35.0 2.0 37.0 82-83 29.244978553982406 34.0 29.0 35.0 2.0 36.5 84-85 28.763373147467846 34.0 29.0 35.0 2.0 36.0 86-87 28.185255697425816 33.5 28.5 35.0 2.0 36.0 88-89 27.429140438319532 33.0 26.0 35.0 2.0 35.0 90-91 26.801360399001833 32.5 25.0 34.5 2.0 35.0 92-93 26.418213890929792 32.0 25.0 34.0 2.0 35.0 94-95 25.832446368408164 32.0 24.0 34.0 2.0 35.0 96-97 24.903258768707346 31.5 19.0 34.0 2.0 35.0 98-99 23.769990633915157 31.0 2.0 34.0 2.0 35.0 100-101 18.258600746639132 23.5 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.029732044825188098 1101 2 0.14886183868355118 1101 3 0.23430519186534582 1101 4 0.10517496279507554 1101 5 0.06641119473429313 1101 6 0.10573853138289735 1101 7 0.12863082063136488 1101 8 0.19453127423554406 1101 9 0.01939188524215041 1101 10-11 0.20636456028792338 1101 12-13 -7.011149415063755E-4 1101 14-15 0.2217396090081678 1101 16-17 0.05617746992436423 1101 18-19 0.180588836123718 1101 20-21 0.30534561120365566 1101 22-23 0.22567857332469288 1101 24-25 0.28646681569282606 1101 26-27 0.3008085053090568 1101 28-29 0.3419720955306289 1101 30-31 0.3895860451627158 1101 32-33 0.26365312770510485 1101 34-35 0.5914133560545167 1101 36-37 0.7069144647291168 1101 38-39 0.7399577779668789 1101 40-41 0.6662712093680554 1101 42-43 0.7558399302062355 1101 44-45 0.8128782288931831 1101 46-47 0.6517299108796166 1101 48-49 0.5697552696621884 1101 50-51 0.7972613310377525 1101 52-53 0.6916345097359482 1101 54-55 0.747136868554584 1101 56-57 0.2825847959163994 1101 58-59 -0.014258820914001546 1101 60-61 -0.02214055190513875 1101 62-63 -0.17425562586863919 1101 64-65 -0.2649676561730274 1101 66-67 -0.20039533527270947 1101 68-69 0.08351462308879576 1101 70-71 -0.06278965180928964 1101 72-73 0.11123790756812824 1101 74-75 -0.04382574685472207 1101 76-77 -0.396450291607259 1101 78-79 0.053156691334617534 1101 80-81 0.06864569708948665 1101 82-83 -0.11898729560487808 1101 84-85 -0.16851040191795974 1101 86-87 0.3631792563248375 1101 88-89 0.25972982997337724 1101 90-91 0.08711492931990605 1101 92-93 0.11480509779791603 1101 94-95 0.1483784721183774 1101 96-97 0.06245804305813252 1101 98-99 0.7175331188036296 1101 100-101 1.2057312586974227 1102 1 0.23361428402341744 1102 2 0.14031029109855098 1102 3 0.2710918253062502 1102 4 0.19649813776137393 1102 5 0.21555263569729277 1102 6 0.1971706432611029 1102 7 0.21604588388285606 1102 8 0.04684005591235518 1102 9 0.09740433128433779 1102 10-11 0.34462933145232455 1102 12-13 0.2947766415027928 1102 14-15 0.4448560217156583 1102 16-17 0.522328728826551 1102 18-19 0.2616174729144376 1102 20-21 0.14061938328040213 1102 22-23 0.2238198372652107 1102 24-25 0.1980440860061563 1102 26-27 0.40895902888708235 1102 28-29 0.30957357707263355 1102 30-31 0.443761733167392 1102 32-33 0.5276787551136337 1102 34-35 0.6315906508786497 1102 36-37 0.6011571489346252 1102 38-39 0.6877974228325456 1102 40-41 0.8487950052747593 1102 42-43 0.8719496569107577 1102 44-45 0.8233804280570922 1102 46-47 0.8000781549527645 1102 48-49 1.1951825066135982 1102 50-51 1.2024283449408273 1102 52-53 1.266477572497223 1102 54-55 1.282861367104367 1102 56-57 1.4639783713620176 1102 58-59 1.4010495386322717 1102 60-61 1.5397322097817465 1102 62-63 1.6352818007691639 1102 64-65 1.2960710572820489 1102 66-67 1.2011052706888634 1102 68-69 1.4147951764883118 1102 70-71 1.561680982884397 1102 72-73 1.4464674240244122 1102 74-75 1.3323470270875681 1102 76-77 1.2600648165807122 1102 78-79 1.4454214897332456 1102 80-81 1.357481706185716 1102 82-83 1.306571346496515 1102 84-85 1.563988202327856 1102 86-87 1.8980254510223347 1102 88-89 1.5740230336717858 1102 90-91 1.8589329502085583 1102 92-93 2.0245985261787034 1102 94-95 1.9153658218194813 1102 96-97 2.079336455847322 1102 98-99 2.514845781357927 1102 100-101 2.4246638131318363 1103 1 0.05072949867200549 1103 2 0.1849334500727693 1103 3 0.08186507300054302 1103 4 0.05239380565819829 1103 5 0.20883622282175196 1103 6 0.13637546855341043 1103 7 0.18848868811173247 1103 8 0.04328113296725178 1103 9 -0.11396870456126607 1103 10-11 0.024778300208019743 1103 12-13 0.07215842116666238 1103 14-15 0.1323151194021932 1103 16-17 0.08193815811699778 1103 18-19 0.19579595205678402 1103 20-21 0.21893761508604115 1103 22-23 0.2715043446495571 1103 24-25 0.06796798240269908 1103 26-27 0.00806238270811832 1103 28-29 0.12977881391810797 1103 30-31 0.4071149469928841 1103 32-33 0.39393240680470853 1103 34-35 0.3257425477339808 1103 36-37 0.484475077135464 1103 38-39 0.5687489202788925 1103 40-41 0.36885990923443757 1103 42-43 0.4126377603252962 1103 44-45 0.5470545184233799 1103 46-47 0.5545074116607438 1103 48-49 0.5921238569128136 1103 50-51 0.7270709346223043 1103 52-53 0.571664491853042 1103 54-55 0.565399368305286 1103 56-57 0.6124512392129731 1103 58-59 0.717778500506256 1103 60-61 0.6895341919837534 1103 62-63 0.620287096986246 1103 64-65 0.6843984646994556 1103 66-67 0.6317197668060928 1103 68-69 0.7049654650478061 1103 70-71 0.7363650652220173 1103 72-73 0.8491536518359624 1103 74-75 0.7347802508982824 1103 76-77 0.559759862425036 1103 78-79 0.6525912857782821 1103 80-81 0.9219876062390142 1103 82-83 0.9456105385029403 1103 84-85 1.1197334171788569 1103 86-87 0.9294822817930175 1103 88-89 0.8668195037599666 1103 90-91 0.7613003929790558 1103 92-93 0.8365963132370418 1103 94-95 0.8868775713252788 1103 96-97 0.5287913165601879 1103 98-99 0.14805238779840124 1103 100-101 0.5311784974840457 1104 1 0.11390235480195088 1104 2 0.01427599442028793 1104 3 -0.006172967967657428 1104 4 0.027759546669983592 1104 5 -0.033691211819729006 1104 6 0.028165566713155954 1104 7 0.07658072019416551 1104 8 0.03895208639828951 1104 9 -0.0038268315249467832 1104 10-11 0.09018235539604547 1104 12-13 0.05852729106108967 1104 14-15 0.22701748525378918 1104 16-17 0.21400172452281652 1104 18-19 0.12702233094430682 1104 20-21 0.18425970785968815 1104 22-23 0.06349219339637813 1104 24-25 0.16076277063393718 1104 26-27 0.08570550794798493 1104 28-29 0.30773482742238656 1104 30-31 0.08274056089787507 1104 32-33 0.19587771315548963 1104 34-35 0.07721429346835862 1104 36-37 0.0121943103315445 1104 38-39 0.18904990447835957 1104 40-41 0.27953876618400386 1104 42-43 -0.07113238063704586 1104 44-45 -0.10688032796214486 1104 46-47 -0.29534362490360166 1104 48-49 -0.38225150492522886 1104 50-51 -0.3088673073489545 1104 52-53 -0.06311164017149196 1104 54-55 0.05299767441538705 1104 56-57 -0.1867424717979702 1104 58-59 -1.9761330440815073E-4 1104 60-61 0.014272855476008317 1104 62-63 0.23381479833081187 1104 64-65 0.2731000109269033 1104 66-67 -0.0400207636078207 1104 68-69 -0.06629434930082567 1104 70-71 -0.0040043510885041655 1104 72-73 -0.05503488773692311 1104 74-75 -0.0771078769110467 1104 76-77 -0.10847124556369536 1104 78-79 0.4441823323602563 1104 80-81 0.5214402144001298 1104 82-83 0.10023165748504326 1104 84-85 0.32319860592008354 1104 86-87 0.3979088636350916 1104 88-89 0.586101486990195 1104 90-91 0.2522454976762809 1104 92-93 0.49974542628663343 1104 94-95 0.7008716778373909 1104 96-97 0.4457539586121122 1104 98-99 0.6777816516328095 1104 100-101 0.8465402704741756 1105 1 0.06522680121035762 1105 2 -0.03803288759473844 1105 3 0.09773286008753246 1105 4 -0.01062362218159052 1105 5 0.012676687232662687 1105 6 0.0904906514089916 1105 7 -0.0976349227576705 1105 8 0.05656022701791841 1105 9 0.2593562587681717 1105 10-11 0.0450450008519212 1105 12-13 0.18235812034374987 1105 14-15 0.05705811470338773 1105 16-17 0.09962859642343602 1105 18-19 0.16805684756432981 1105 20-21 0.24020498698769188 1105 22-23 0.20941145003701678 1105 24-25 -0.09415940739915385 1105 26-27 0.037103602905524724 1105 28-29 0.22710807826934598 1105 30-31 0.35671623232714467 1105 32-33 0.2005666707830258 1105 34-35 0.19685329283007036 1105 36-37 0.00916244505928887 1105 38-39 0.04911152376446637 1105 40-41 0.2853815510811373 1105 42-43 0.34643341206683687 1105 44-45 0.4302354721065882 1105 46-47 0.36024200380951044 1105 48-49 0.360800981940784 1105 50-51 0.2946426597547003 1105 52-53 0.282582374656414 1105 54-55 0.4482602314930162 1105 56-57 0.18318191569160547 1105 58-59 0.10933619137963291 1105 60-61 0.08403951232286744 1105 62-63 0.47253736424626425 1105 64-65 0.41870626123377264 1105 66-67 0.21649713298962325 1105 68-69 0.47869877683459094 1105 70-71 0.8045738636706687 1105 72-73 0.6021556656091747 1105 74-75 0.4684867123755474 1105 76-77 0.3107853950804831 1105 78-79 0.6639311908077481 1105 80-81 0.6264446333130778 1105 82-83 0.41287895458329515 1105 84-85 0.60520503789337 1105 86-87 0.7012795122333166 1105 88-89 1.0515940730362523 1105 90-91 0.8227639834621137 1105 92-93 1.2205806008336921 1105 94-95 1.4409107115684279 1105 96-97 0.9111176596972008 1105 98-99 1.044318205862691 1105 100-101 1.051866288051631 1106 1 0.16873736939752604 1106 2 0.2187987745998683 1106 3 0.16117728884053406 1106 4 0.06635467655210903 1106 5 0.13759430715451515 1106 6 0.10942041650807965 1106 7 0.21946953518141754 1106 8 0.2865595209029621 1106 9 0.31686932189484907 1106 10-11 0.29057588161264647 1106 12-13 0.259863063148444 1106 14-15 0.19224676508233784 1106 16-17 0.0771358929446464 1106 18-19 0.10297658583105829 1106 20-21 0.1598167414479903 1106 22-23 0.07705314377142969 1106 24-25 0.3175173802485389 1106 26-27 0.08331294245266463 1106 28-29 0.27098723846329875 1106 30-31 0.12814387908137093 1106 32-33 0.21046404874282842 1106 34-35 0.12016038222421344 1106 36-37 0.12106989692578196 1106 38-39 0.08837739958237734 1106 40-41 0.348590608182306 1106 42-43 0.6066102938631985 1106 44-45 0.6940194244673705 1106 46-47 0.9096318028021244 1106 48-49 0.8821962650928796 1106 50-51 0.9043758659873404 1106 52-53 0.7243749651568123 1106 54-55 0.6973275713226101 1106 56-57 0.7350695963393434 1106 58-59 0.7770443776056908 1106 60-61 0.8334540253389235 1106 62-63 0.8078919516397605 1106 64-65 0.9141135718772802 1106 66-67 0.9249775736053536 1106 68-69 0.920662011782575 1106 70-71 0.6870498557621758 1106 72-73 0.8297247471889904 1106 74-75 1.3982459271757115 1106 76-77 0.9533382360163181 1106 78-79 1.156381645075033 1106 80-81 1.0540031231685596 1106 82-83 0.9127524423204285 1106 84-85 0.7861646481179143 1106 86-87 1.1537932814749468 1106 88-89 1.1254448709461506 1106 90-91 1.2940368733552496 1106 92-93 1.55705614571907 1106 94-95 1.460017005406396 1106 96-97 1.4630377039470694 1106 98-99 2.061100204638759 1106 100-101 1.743900134495167 1107 1 -0.02057341348708519 1107 2 0.020375900998296004 1107 3 0.1686238544633909 1107 4 0.19431325210371853 1107 5 0.2025351502790329 1107 6 0.21140089854441158 1107 7 0.17777871986444183 1107 8 0.1506157916488533 1107 9 0.207893049041445 1107 10-11 0.14070350357064854 1107 12-13 0.2383005137534724 1107 14-15 0.21503138253844867 1107 16-17 0.23231365628201672 1107 18-19 0.24142152983545628 1107 20-21 0.14723895352289418 1107 22-23 0.06256102647630968 1107 24-25 0.15559555747914544 1107 26-27 0.17919771972892562 1107 28-29 0.3270969933471761 1107 30-31 0.35806830112368004 1107 32-33 0.3621673335446971 1107 34-35 0.3884255431859458 1107 36-37 0.4070411410057133 1107 38-39 0.4894367203759771 1107 40-41 0.7203363946087151 1107 42-43 0.7405826151192798 1107 44-45 0.6414222005376686 1107 46-47 0.6526316213213548 1107 48-49 0.6058127669633038 1107 50-51 0.486640804488772 1107 52-53 0.46923496152719935 1107 54-55 0.4999028419338529 1107 56-57 0.46930125946420276 1107 58-59 0.42252316484583474 1107 60-61 0.5836325789957613 1107 62-63 0.6467033697070548 1107 64-65 0.4536456442120027 1107 66-67 0.4128286071675902 1107 68-69 0.5096461292884413 1107 70-71 0.5193923626555232 1107 72-73 0.31609466353221194 1107 74-75 0.33743084436779824 1107 76-77 0.3930705226154281 1107 78-79 0.6193668865908073 1107 80-81 0.7384402693542462 1107 82-83 0.5327434268243145 1107 84-85 0.9932327385304625 1107 86-87 1.2408118452010406 1107 88-89 1.4306882015280031 1107 90-91 1.1712797707249543 1107 92-93 1.1618188449820224 1107 94-95 1.7383045880240502 1107 96-97 1.8675466228485824 1107 98-99 1.6573494070015222 1107 100-101 2.0371379269865457 1108 1 0.04116918924106017 1108 2 0.08033639249853053 1108 3 0.12299274544650984 1108 4 0.021315559786735605 1108 5 0.11377694954685325 1108 6 0.2290921281407492 1108 7 0.3146841290250961 1108 8 0.29343237313663906 1108 9 0.4344024066356553 1108 10-11 0.18066508246078428 1108 12-13 0.1762921046049044 1108 14-15 0.28276369130885115 1108 16-17 0.10333864208553933 1108 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66-67 -1.012345568947083 2316 68-69 -1.0994991447525635 2316 70-71 -0.9131812142698195 2316 72-73 -0.7814815568754767 2316 74-75 -0.868341346197905 2316 76-77 -1.1248499379379417 2316 78-79 -1.0436337595937637 2316 80-81 -1.2537655698045427 2316 82-83 -1.107892183820109 2316 84-85 -1.2116277443456482 2316 86-87 -1.5350549232886515 2316 88-89 -2.048643291158367 2316 90-91 -2.1679918030282046 2316 92-93 -2.361044340120486 2316 94-95 -2.1414345883843353 2316 96-97 -1.7820979756358213 2316 98-99 -2.1102779207315443 2316 100-101 -1.5003278546030678 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 2.0 4 11.0 5 62.0 6 144.0 7 222.0 8 312.0 9 517.0 10 890.0 11 1278.0 12 1823.0 13 2381.0 14 2721.0 15 3700.0 16 4860.0 17 6719.0 18 6654.0 19 4319.0 20 4011.0 21 4375.0 22 4929.0 23 5532.0 24 6245.0 25 7312.0 26 8314.0 27 9646.0 28 11573.0 29 13969.0 30 16745.0 31 20413.0 32 24675.0 33 31271.0 34 43352.0 35 60731.0 36 83660.0 37 128680.0 38 78894.0 39 3362.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.15446587265993 24.301589674590122 16.983272703111243 17.560671749638708 2 13.87669863711882 26.779059684796497 36.98163850222072 22.362603175863963 3 21.70515697293433 28.280777351946355 33.59214175552864 16.42192391959067 4 10.805748062246403 20.54780012841134 43.051887448122486 25.594564361219774 5 9.072029494893332 42.259079806985845 37.08622093369606 11.582669764424763 6 22.92357539532821 42.33999880855458 17.855795389106216 16.880630407010994 7 21.002700609622906 37.24524580180967 25.123116026926667 16.628937561640754 8 21.918127842093767 31.810103457177462 22.28731044434295 23.984458256385814 9 20.52364026291229 22.435082772361113 27.778219053859953 29.263057910866646 10-11 22.260089226023815 27.75099783554082 29.290444607716594 20.69846833071877 12-13 26.24175089523885 27.636652170747368 28.24222085426637 17.879376079747413 14-15 21.446555503061784 30.083922922963506 31.017971899927932 17.451549674046777 16-17 16.41765852084445 32.61795721478246 31.805029807338276 19.159354457034823 18-19 16.05680882663214 34.27567918650687 32.3150051918932 17.35250679496779 20-21 18.541124724478248 30.03253307915831 30.680216048769832 20.74612614759361 22-23 17.359608014456203 29.869867683366756 32.47582358664787 20.294700715529167 24-25 20.743892187427605 30.87506701880498 30.88979460804755 17.491246185719863 26-27 19.197329838426764 28.151786837175745 35.863582808766395 16.787300515631102 28-29 16.2225222899581 33.29767270994261 33.063851545900434 17.415953454198853 30-31 17.763789325972848 29.97271259026854 32.152147580372926 20.11135050338569 32-33 18.354961377310907 30.77619359664277 33.11415701926832 17.754688006778 34-35 20.57381783397959 29.126306783108348 32.246036829066064 18.053838553846003 36-37 18.98778278633241 28.5641815426808 34.83772034720952 17.610315323777264 38-39 17.721179624664877 31.052196074537452 33.583391255419855 17.64323304537782 40-41 17.148095989091093 31.50277108015033 33.30633136789916 18.042801562859417 42-43 16.768287889933003 31.557961331255953 30.691650400927372 20.98210037788367 44-45 18.369307373885388 28.94234086792768 31.573808769298108 21.114542988888825 46-47 20.632698467070604 31.39721049378579 31.397624195247232 16.572466843896372 48-49 18.890820437419897 30.154304158492522 32.123545925944924 18.83132947814266 50-51 15.07534694843411 31.17451549674047 35.07668733229688 18.673450222528544 52-53 17.656062802412016 33.20690773579036 30.16789451736532 18.96913494443231 54-55 16.525182522819488 29.96865836626356 31.73282498328667 21.77333412763028 56-57 19.25872237335928 29.70654037345195 30.594829126869076 20.439908126319693 58-59 21.339094230718313 29.14336161931743 30.806514601922213 18.711029548042045 60-61 18.35935657732631 31.245351022133892 33.004578027948064 17.390714372591738 62-63 16.920197551375686 30.852550519321163 32.932565611200424 19.294686318102723 64-65 16.749171982005763 33.26667267908811 31.001506690749448 18.982648648156683 66-67 18.139635327418457 30.188045418177033 29.111171603958656 22.56114765044585 68-69 16.667590607575267 30.193340840697967 31.371572351606126 21.767496200120636 70-71 20.086115027436342 30.369364628633083 29.865730720096373 19.678789623834202 72-73 18.96524619895815 27.739331599118334 33.49988747459904 19.795534727324476 74-75 16.57954222019235 33.36527068978071 30.096656009849283 19.958531080177657 76-77 17.93113614267661 29.68923648204369 30.32366576069404 22.055961614585662 78-79 18.067454256310285 29.98695992493683 31.54589427941174 20.399691539341145 80-81 19.762273542630577 29.291189261105266 30.1688046492848 20.777732546979355 82-83 18.051970187387887 29.120911852896203 32.54970975065695 20.277408209058954 84-85 17.443991491058743 30.00535325261809 31.300393592313817 21.250261664009347 86-87 17.333596135483344 30.754973710123156 30.81998411592887 21.09144603846463 88-89 17.579375960779426 30.111678923096097 29.22024180481117 23.088703311313306 90-91 18.339158399723587 27.665383158692087 30.690403258493408 23.305055183090918 92-93 19.678624145303388 30.08308677032242 30.60020717912058 19.638081905253614 94-95 18.57855722681476 28.44134922716558 31.116090229794235 21.86400331622543 96-97 16.234271265645994 29.120580895834575 32.5147937806549 22.130354057864533 98-99 17.920497494655045 30.026493112783548 29.16972801948675 22.883281373074656 100-101 17.28719993447023 27.761868789875322 31.11588426714634 23.835047008508113 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10141.0 1 6085.0 2 1779.0 3 1365.5 4 1031.0 5 1034.5 6 1685.0 7 2784.0 8 3579.5 9 3557.0 10 2739.0 11 1859.0 12 1611.0 13 1522.0 14 1358.0 15 1211.0 16 1129.0 17 1118.0 18 1156.5 19 1231.5 20 1359.5 21 1606.0 22 1842.0 23 1984.5 24 2122.5 25 2680.5 26 4133.0 27 5693.5 28 5952.0 29 6339.0 30 8103.0 31 9996.0 32 12850.5 33 17083.5 34 20984.5 35 23195.0 36 26323.0 37 29497.5 38 28292.0 39 25435.0 40 23077.5 41 20727.0 42 18278.5 43 16893.0 44 16340.0 45 16081.5 46 17948.5 47 31409.5 48 57590.5 49 55645.0 50 28732.0 51 12584.5 52 4724.5 53 1708.0 54 872.5 55 589.0 56 444.5 57 315.5 58 260.5 59 259.0 60 246.0 61 241.5 62 205.0 63 151.5 64 121.0 65 95.0 66 80.0 67 73.5 68 49.5 69 36.5 70 33.0 71 28.5 72 19.0 73 16.0 74 19.5 75 13.5 76 6.0 77 4.5 78 2.5 79 1.5 80 1.0 81 1.0 82 1.5 83 0.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3802696638138168 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 5.79174857853942E-4 16-17 0.0017375245735618261 18-19 8.273926540770601E-5 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.009432276256478484 36-37 0.019774684432441736 38-39 0.007942969479139778 40-41 0.005129834455277773 42-43 0.0035577884125313586 44-45 0.001075610450300178 46-47 0.00124108898111559 48-49 0.002399438696823474 50-51 5.79174857853942E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 6.619141232616481E-4 60-61 0.001406567511931002 62-63 0.0015720460427464142 64-65 5.79174857853942E-4 66-67 0.0020684816351926503 68-69 0.0020684816351926503 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.08886197104787626 78-79 0.002813135023862004 80-81 0.0 82-83 0.0 84-85 4.1369632703853E-4 86-87 0.0924197594604076 88-89 0.4274310450962092 90-91 0.3825863632452326 92-93 0.0 94-95 0.001075610450300178 96-97 0.0 98-99 0.0 100-101 4.1369632703853E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 604308.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.010888487327653 #Duplication Level Percentage of deduplicated Percentage of total 1 70.3831907575624 10.565142278440796 2 10.262148337595908 3.080879286721341 3 4.1471911103271895 1.86759069878274 4 2.427462739218626 1.457534899422149 5 1.6172061028309375 1.213785023531047 6 1.264441308757386 1.1388232490716654 7 0.9612840638504276 1.0100809520972749 8 0.7209630478878207 0.8657836732262357 9 0.6382837992768322 0.862308624079112 >10 5.6673868947879 18.474850572886673 >50 1.1001852015168887 11.486692216551825 >100 0.7419084575359379 20.505106667460964 >500 0.0319693094629156 3.1962178226996834 >1k 0.024252579592556663 9.529908589659577 >5k 0.009921509833318635 9.567968651747123 >10k+ 0.002204779962959697 5.177326793621796 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 19693 3.258768707347909 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11594 1.9185580862738867 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8953 1.4815292863903837 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7943 1.3143959702668175 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7808 1.292056368606737 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6004 0.9935330990157337 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5766 0.9541492086816656 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5483 0.9073187844609041 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5360 0.8869649251706083 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5359 0.8867994466397929 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5144 0.8512215625144793 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4797 0.7938005123215315 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4794 0.7933040767290851 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4312 0.7135434248760566 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4248 0.7029527989038702 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3805 0.6296458097526426 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3439 0.5690806674742019 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3056 0.5057023901718991 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3044 0.5037166478021142 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3033 0.5018963839631446 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2896 0.47922582524143315 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2820 0.4666494568994619 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2231 0.3691826022491842 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1950 0.3226831350900534 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1940 0.3210283497818993 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1760 0.29124221423512514 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1694 0.28032063120130796 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1639 0.27121931200646027 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1408 0.2329937713881001 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1337 0.22124479570020586 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1188 0.19658849460870947 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1126 0.18632882569815393 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1073 0.17755846356493707 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 990 0.1638237455072579 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 980 0.16216896019910376 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 935 0.15472242631241023 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 932 0.15422599071996398 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 855 0.14148414384717725 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 805 0.13321021730640667 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 793 0.13122447493662173 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 749 0.1239434195807436 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 746 0.12344698398829737 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 720 0.11914454218709665 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 697 0.11533853597834216 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 665 0.11004322299224899 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 626 0.10358956029044791 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 614 0.10160381792066298 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 611 0.10110738232821674 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.4821779622311805E-4 0.0 12-13 0.0 0.0 0.0 6.619141232616481E-4 0.0 14-15 0.0 0.0 0.0 9.928711848924722E-4 0.0 16-17 0.0 0.0 0.0 0.0013238282465232961 0.0 18-19 0.0 0.0 0.0 0.0018202638389695322 0.0 20-21 0.0 0.0 0.0 0.00330957061630824 0.0 22-23 0.0 0.0 0.0 0.005791748578539421 0.0 24-25 0.0 0.0 0.0 0.011335279360855722 0.0 26-27 0.0 0.0 0.0 0.018119899124287613 0.0 28-29 0.0 0.0 0.0 0.04583755303586913 0.0 30-31 0.0 0.0 0.0 0.10044546820495509 0.0 32-33 0.0 0.0 0.0 0.20742733837711896 0.0 34-35 0.0 0.0 0.0 0.34105125201056413 0.0 36-37 0.0 0.0 0.0 0.528538427424426 0.0 38-39 0.0 0.0 0.0 0.7805622298562984 0.0 40-41 0.0 0.0 0.0 1.05136784553572 0.0 42-43 0.0 0.0 0.0 1.3214288078264727 0.0 44-45 0.0 0.0 0.0 1.6357552771103476 0.0 46-47 0.0 0.0 0.0 2.020410121990773 0.0 48-49 0.0 0.0 0.0 2.341024775445634 0.0 50-51 0.0 0.0 0.0 2.6925839141629764 0.0 52-53 0.0 0.0 0.0 3.0198177088504536 0.0 54-55 0.0 0.0 0.0 3.3927235780429847 0.0 56-57 0.0 0.0 0.0 3.9224203551831183 0.0 58-59 0.0 0.0 0.0 4.391800207841035 0.0 60-61 0.0 0.0 0.0 4.914546886686921 0.0 62-63 0.0 0.0 0.0 5.409327693825003 0.0 64-65 0.0 0.0 0.0 5.9410929526003295 0.0 66-67 0.0 0.0 0.0 6.571648894272457 0.0 68-69 0.0 0.0 0.0 7.28676436519126 0.0 70-71 0.0 0.0 0.0 8.049703131515717 0.0 72-73 0.0 0.0 0.0 8.720139399114359 0.0 74-75 0.0 0.0 0.0 9.537106905750047 0.0 76-77 0.0 0.0 0.0 10.276382242167902 0.0 78-79 0.0 0.0 0.0 11.312939759195643 0.0 80-81 0.0 0.0 0.0 12.35355150022836 0.0 82-83 0.0 0.0 0.0 13.234972894616652 0.0 84-85 0.0 0.0 0.0 14.212289097612476 0.0 86-87 0.0 0.0 0.0 15.2025126260119 0.0 88-89 0.0 0.0 0.0 16.2643883582544 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTTGC 505 0.0 39.627823 1 GTAAGCG 175 0.0 39.31621 94-95 CTTTGCG 525 0.0 38.861084 2 CTCCGAC 110 0.0 34.50953 94-95 TTTGCGT 610 0.0 33.446014 3 TTGCGTT 615 0.0 33.174095 4 ACTGCAC 60 0.003971259 31.631113 3 AGGGGTG 140 1.7218554E-8 30.501434 8 GTAGGGG 145 2.4223482E-8 29.44966 6 TGTAGAT 130 3.0628144E-7 29.197952 5 CCTTTAG 135 4.1064231E-7 28.235695 1 GTAGATA 135 4.2611646E-7 28.116549 6 TAGATAT 135 4.2611646E-7 28.116549 7 TGGGATA 85 6.445834E-4 27.909807 6 CATTCCG 120 5.4198154E-6 27.679514 9 GGGTAGG 70 0.00826234 27.227278 1 GTATAGT 210 4.1836756E-11 27.227278 1 TGAAAGC 35 0.008434437 27.11463 94-95 AAGCAGG 390 0.0 26.767006 94-95 AAGACAT 130 1.0098431E-5 25.548208 5 >>END_MODULE