##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939058_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 609242 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.802605532776795 33.0 31.0 34.0 30.0 34.0 2 31.849294697345226 33.0 31.0 34.0 30.0 34.0 3 31.866173047820077 34.0 31.0 34.0 30.0 34.0 4 35.37716867845618 37.0 35.0 37.0 33.0 37.0 5 35.44149615423756 37.0 35.0 37.0 33.0 37.0 6 35.57908351689476 37.0 35.0 37.0 33.0 37.0 7 35.529887959136104 37.0 35.0 37.0 33.0 37.0 8 35.53636157717295 37.0 35.0 37.0 33.0 37.0 9 37.0860774536227 39.0 37.0 39.0 33.0 39.0 10-11 36.99510457256723 39.0 37.0 39.0 33.0 39.0 12-13 36.931368815675874 39.0 37.0 39.0 33.0 39.0 14-15 38.027040158098096 40.0 38.0 41.0 33.0 41.0 16-17 37.97466195698918 40.0 38.0 41.0 33.0 41.0 18-19 37.95113107763417 40.0 38.0 41.0 33.0 41.0 20-21 37.82352414968108 40.0 37.0 41.0 33.0 41.0 22-23 37.88962678213255 40.0 37.5 41.0 33.0 41.0 24-25 37.7768046195108 40.0 37.0 41.0 33.0 41.0 26-27 37.636724815426376 40.0 37.0 41.0 33.0 41.0 28-29 37.55543774066791 40.0 37.0 41.0 32.5 41.0 30-31 37.413017323165505 40.0 36.5 41.0 32.5 41.0 32-33 37.15541525370871 40.0 35.5 41.0 32.0 41.0 34-35 36.796236142616564 39.0 35.0 41.0 31.0 41.0 36-37 36.47199142541059 39.0 35.0 41.0 30.0 41.0 38-39 36.244866571904105 39.0 35.0 41.0 30.0 41.0 40-41 36.23296243528844 39.0 35.0 41.0 30.0 41.0 42-43 36.01251309003647 39.0 35.0 41.0 30.0 41.0 44-45 35.847055029036085 39.0 35.0 41.0 29.5 41.0 46-47 35.55157966784955 39.0 35.0 41.0 27.0 41.0 48-49 35.28907314334862 39.0 35.0 41.0 25.5 41.0 50-51 34.11714720915498 38.0 34.0 40.0 23.5 40.5 52-53 34.39179505024276 38.0 34.5 39.5 23.5 40.5 54-55 34.96678577642382 39.0 35.0 40.5 24.0 41.0 56-57 34.85954267762236 39.0 35.0 41.0 23.0 41.0 58-59 34.72137590645425 39.0 35.0 41.0 23.0 41.0 60-61 34.377764500805924 38.5 34.5 40.0 22.0 41.0 62-63 34.101263537313585 38.0 34.0 40.0 22.0 41.0 64-65 33.70962605992364 37.5 34.0 40.0 20.5 41.0 66-67 33.22675718351657 37.0 33.0 39.0 18.0 41.0 68-69 32.768387602955805 36.5 33.0 39.0 14.5 41.0 70-71 32.19280926134442 36.0 32.0 38.5 10.0 40.0 72-73 31.681612561182583 35.0 31.5 37.5 7.0 40.0 74-75 31.184974115376157 35.0 31.0 37.0 2.0 39.0 76-77 30.753794879538837 35.0 31.0 36.5 2.0 39.0 78-79 30.24153620400432 35.0 31.0 36.0 2.0 38.5 80-81 29.693026744708998 34.0 30.0 36.0 2.0 37.0 82-83 29.216516589466913 34.0 29.5 35.0 2.0 37.0 84-85 28.75954710935884 34.0 29.0 35.0 2.0 36.5 86-87 28.440297944002545 34.0 29.0 35.0 2.0 36.0 88-89 27.90882276665102 34.0 28.0 35.0 2.0 36.0 90-91 27.386479428535786 34.0 26.5 35.0 2.0 35.0 92-93 26.894849665650103 34.0 25.5 35.0 2.0 35.0 94-95 26.337433236710538 33.5 25.0 35.0 2.0 35.0 96-97 25.66879253235988 33.0 21.5 35.0 2.0 35.0 98-99 25.030886741229267 33.0 10.0 35.0 2.0 35.0 100-101 22.46198554925629 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2098329899054825 1101 2 0.3296582468328886 1101 3 0.26788219198762775 1101 4 0.23265487159147824 1101 5 0.021344378622991655 1101 6 0.06508748701495648 1101 7 -0.004038400386228602 1101 8 0.18244168111367287 1101 9 0.06687840887931884 1101 10-11 0.3311887230583679 1101 12-13 0.5303314803403865 1101 14-15 0.22899317587484802 1101 16-17 0.3348817548475438 1101 18-19 0.3576221651670366 1101 20-21 0.576482134305806 1101 22-23 0.37405777298633325 1101 24-25 0.48867175037104715 1101 26-27 0.5039149909293386 1101 28-29 0.3461390813523124 1101 30-31 0.3404903734948519 1101 32-33 0.2237438200937305 1101 34-35 0.20446191318749385 1101 36-37 -0.14943849102002815 1101 38-39 0.1589554693760178 1101 40-41 -0.11125670799300735 1101 42-43 -0.19771725219766978 1101 44-45 -0.05236309936535832 1101 46-47 0.06187904805582889 1101 48-49 0.4692052927724717 1101 50-51 0.22162658207823682 1101 52-53 0.10689103230100017 1101 54-55 0.06840039170968026 1101 56-57 0.14188546104026045 1101 58-59 0.39120834793397563 1101 60-61 0.37353389214585775 1101 62-63 0.3222562673653684 1101 64-65 0.28453872311857964 1101 66-67 0.4153828632599783 1101 68-69 0.4764484533357489 1101 70-71 0.4249144830982061 1101 72-73 0.39395323926627057 1101 74-75 0.446912232741262 1101 76-77 0.43882465455185127 1101 78-79 0.5193617377591764 1101 80-81 0.34396798625073544 1101 82-83 0.26778962840964127 1101 84-85 0.2283484939980056 1101 86-87 0.17415812859995938 1101 88-89 0.06744389157977082 1101 90-91 0.05117195470435121 1101 92-93 0.40678095714503115 1101 94-95 0.6016408571406231 1101 96-97 0.5556636299268867 1101 98-99 0.7241565072767706 1101 100-101 0.9198619592791957 1102 1 0.20719399540711336 1102 2 0.21731567595383794 1102 3 0.04724499941770688 1102 4 0.24840257604965643 1102 5 0.367186844070055 1102 6 0.3081689124553719 1102 7 0.4127054765714533 1102 8 0.2890877299702126 1102 9 0.5101368322558244 1102 10-11 0.37531360564547356 1102 12-13 0.39846642049975145 1102 14-15 0.5480798793042609 1102 16-17 0.5401370599932633 1102 18-19 0.5059457966279339 1102 20-21 0.7461956788012998 1102 22-23 0.6996509077667454 1102 24-25 0.765751795610953 1102 26-27 0.7982651145722954 1102 28-29 0.906000486217529 1102 30-31 0.8795736903211591 1102 32-33 0.9036427331858263 1102 34-35 1.0238872769278373 1102 36-37 1.0578232167225181 1102 38-39 0.786318903008187 1102 40-41 0.8773566377791724 1102 42-43 0.8846538811642048 1102 44-45 1.1272434795904118 1102 46-47 1.2418562364084664 1102 48-49 1.3692637595997326 1102 50-51 1.3185585113557323 1102 52-53 1.5100725222785698 1102 54-55 1.6324535166497824 1102 56-57 1.5126929422382247 1102 58-59 1.3371584818006355 1102 60-61 1.3657766540804488 1102 62-63 1.36408720558223 1102 64-65 1.4050139338228007 1102 66-67 1.4396753507519122 1102 68-69 1.2336785314832213 1102 70-71 1.285539790608162 1102 72-73 1.2857560622875077 1102 74-75 1.1582442540321338 1102 76-77 1.441760775549426 1102 78-79 1.2884289522461678 1102 80-81 1.1085096127148013 1102 82-83 0.8317069697345083 1102 84-85 0.7663982578942594 1102 86-87 0.9243705900214358 1102 88-89 0.8978134147077128 1102 90-91 0.5484083587708319 1102 92-93 0.8024032933899896 1102 94-95 0.7790365593495814 1102 96-97 0.7743870722492012 1102 98-99 1.0023899145189894 1102 100-101 0.8905119314834913 1103 1 0.3511201692785768 1103 2 0.3416395471058813 1103 3 0.43077384861691215 1103 4 0.4981153571360579 1103 5 0.5719661667923361 1103 6 0.4616462796049845 1103 7 0.24241726403566588 1103 8 0.3601876076304791 1103 9 0.6159048090547472 1103 10-11 0.49538974194560126 1103 12-13 0.5979034714290989 1103 14-15 0.5643519951606137 1103 16-17 0.48018282871527873 1103 18-19 0.39874948793436005 1103 20-21 0.6147984597013973 1103 22-23 0.5848285487834701 1103 24-25 0.5587321653626063 1103 26-27 0.5242116949594404 1103 28-29 0.45349407424934185 1103 30-31 0.5491477369096245 1103 32-33 0.5937133424625287 1103 34-35 0.292567374054407 1103 36-37 0.26045227892947054 1103 38-39 0.3438822308973002 1103 40-41 0.6391667689994023 1103 42-43 0.4314503811300554 1103 44-45 0.6963887161035487 1103 46-47 0.425252499147021 1103 48-49 0.3004787111295997 1103 50-51 0.36956703787613066 1103 52-53 0.4030532025371585 1103 54-55 0.3020850070209562 1103 56-57 0.39009859470644415 1103 58-59 0.6063664407143605 1103 60-61 0.3185454444075333 1103 62-63 0.4482966859799262 1103 64-65 0.48577212965331285 1103 66-67 0.5755143715372455 1103 68-69 0.7024151408717074 1103 70-71 0.35033327244661194 1103 72-73 0.4683977931773562 1103 74-75 0.3196069447610199 1103 76-77 0.2497060269668907 1103 78-79 0.44699087043380104 1103 80-81 0.3104664274104749 1103 82-83 0.4145160638615799 1103 84-85 0.3791635084423035 1103 86-87 0.5501786843161831 1103 88-89 0.3675420937491154 1103 90-91 0.3346615958056134 1103 92-93 0.21038456361175406 1103 94-95 0.23236891976118912 1103 96-97 0.16979902179803474 1103 98-99 0.30597099098442726 1103 100-101 0.4655164338340292 1104 1 0.16133199057170344 1104 2 0.04302393954568373 1104 3 0.23198866069103374 1104 4 0.06147058175295683 1104 5 -0.14914196381538147 1104 6 -0.01737817080046966 1104 7 0.0989183585998461 1104 8 0.11120663146423482 1104 9 0.35851573003029813 1104 10-11 0.154140088814529 1104 12-13 0.12327269413437136 1104 14-15 0.27207715163703483 1104 16-17 0.10829154514607353 1104 18-19 0.17964240564163703 1104 20-21 0.2394354035548787 1104 22-23 0.09360664516677986 1104 24-25 0.17295841640321186 1104 26-27 0.24227354554371772 1104 28-29 0.1376352267791745 1104 30-31 0.23422312309036641 1104 32-33 0.21861390670265735 1104 34-35 0.28617727754775757 1104 36-37 0.3003283310985623 1104 38-39 0.3269704435202456 1104 40-41 0.3173448592161563 1104 42-43 0.1489865325807287 1104 44-45 0.21898298737045963 1104 46-47 0.574259683187961 1104 48-49 0.5291732506418327 1104 50-51 0.39843886595247113 1104 52-53 0.4252327727280161 1104 54-55 0.4269454886291939 1104 56-57 0.3369474831842325 1104 58-59 0.29956626805071807 1104 60-61 0.46460809664445435 1104 62-63 0.300730935047234 1104 64-65 0.43618683072937614 1104 66-67 0.5845113807562541 1104 68-69 0.2707221121663821 1104 70-71 -0.15775262771681042 1104 72-73 -0.07582722455149948 1104 74-75 -0.03224610614392631 1104 76-77 0.008359250535477258 1104 78-79 -0.0657307402713414 1104 80-81 0.005967922800973469 1104 82-83 0.11239322601123902 1104 84-85 0.3068390241207801 1104 86-87 0.27807773774941325 1104 88-89 0.20196690798153583 1104 90-91 0.26391266724519724 1104 92-93 0.6161842121179077 1104 94-95 0.7180917946108814 1104 96-97 0.6059412226428513 1104 98-99 0.36054811921801644 1104 100-101 0.7382957018635032 1105 1 0.18528112515312145 1105 2 0.1022197478584097 1105 3 0.2507335093899634 1105 4 0.35723768407251555 1105 5 0.2483854116703128 1105 6 0.3422061927930784 1105 7 0.32891364883886354 1105 8 0.3353264948811656 1105 9 0.4477539261918366 1105 10-11 0.3748659807255166 1105 12-13 0.5013134335668425 1105 14-15 0.7076240726308001 1105 16-17 0.6439297177588159 1105 18-19 0.5832159367849314 1105 20-21 0.5597020937703476 1105 22-23 0.5992495275405005 1105 24-25 0.7163529766292882 1105 26-27 0.5703054541743526 1105 28-29 0.7679778618904436 1105 30-31 0.8130910142948835 1105 32-33 0.7769678452402786 1105 34-35 0.8062526337234317 1105 36-37 0.7825872502084152 1105 38-39 0.7687607414126276 1105 40-41 0.9414500046300276 1105 42-43 0.8649761711065764 1105 44-45 0.7412307207979936 1105 46-47 0.8031393487949288 1105 48-49 0.9877420720241474 1105 50-51 1.0085560212568438 1105 52-53 0.932992103941956 1105 54-55 0.9775930807638957 1105 56-57 0.9379247585875063 1105 58-59 0.7336237271346704 1105 60-61 0.9828142664313333 1105 62-63 0.8987381174327354 1105 64-65 0.9738295953590708 1105 66-67 0.9143800961689408 1105 68-69 0.9595364298397584 1105 70-71 0.6591554142000646 1105 72-73 0.9170168823600768 1105 74-75 1.0662202779370773 1105 76-77 0.6912716489812567 1105 78-79 0.6101022987019284 1105 80-81 0.5156141020073797 1105 82-83 0.4117132542698094 1105 84-85 0.36393898879757103 1105 86-87 0.22361229562060103 1105 88-89 0.2522700988445976 1105 90-91 0.32656426267672956 1105 92-93 0.4230634067487813 1105 94-95 0.4375283894536892 1105 96-97 0.32790048321038157 1105 98-99 0.48037032574103833 1105 100-101 1.042820016004562 1106 1 0.14155527605814555 1106 2 -0.014037906989848636 1106 3 0.12321673086647067 1106 4 0.10554288371999831 1106 5 0.2103653904288123 1106 6 -0.04020290941468119 1106 7 0.05073476831099555 1106 8 0.0066877585243076965 1106 9 0.17854053952878957 1106 10-11 0.0832558074039369 1106 12-13 0.02974088481308712 1106 14-15 -0.10133238229881414 1106 16-17 0.11578755790524298 1106 18-19 0.172695746063404 1106 20-21 0.09893389726389756 1106 22-23 0.21292261185117667 1106 24-25 -0.036850196036972704 1106 26-27 0.15700861554194745 1106 28-29 0.1580587603606034 1106 30-31 0.18184849966311134 1106 32-33 0.3383817606810311 1106 34-35 0.22849519354988246 1106 36-37 -0.09016431465724395 1106 38-39 -0.0029072810943233662 1106 40-41 0.23771522958652724 1106 42-43 0.23008701104962626 1106 44-45 0.43034219346650815 1106 46-47 0.33746188060068505 1106 48-49 0.5065345832663155 1106 50-51 0.4064753713574021 1106 52-53 0.473017638933932 1106 54-55 0.23181878297617686 1106 56-57 0.5198909529698028 1106 58-59 0.4221872143558798 1106 60-61 0.6180282632225129 1106 62-63 0.5117445802714045 1106 64-65 0.254980025838357 1106 66-67 0.4706446432053042 1106 68-69 0.4320015734458309 1106 70-71 0.5890132769438381 1106 72-73 0.3257942533857694 1106 74-75 0.1034352690111362 1106 76-77 0.07514110415943875 1106 78-79 0.33456165738947163 1106 80-81 0.2740965777717328 1106 82-83 0.12032340499404981 1106 84-85 -0.05959726863130044 1106 86-87 -0.17362348500444114 1106 88-89 0.1277333442742865 1106 90-91 0.22999453859022623 1106 92-93 0.4059148518485465 1106 94-95 0.5186500083269863 1106 96-97 0.5301127626038671 1106 98-99 0.36190378109999344 1106 100-101 0.702224463160686 1107 1 0.12253857847002081 1107 2 -0.09204587785496088 1107 3 0.18614190093449778 1107 4 0.08848454523308646 1107 5 0.15257661835433112 1107 6 0.17451226958620936 1107 7 0.12151055337249517 1107 8 0.2811672472895097 1107 9 0.07079643178467876 1107 10-11 0.017951402813814354 1107 12-13 0.15019714812648743 1107 14-15 -0.005097928921500738 1107 16-17 0.3388858881464287 1107 18-19 0.31468537019588183 1107 20-21 0.3908496534653736 1107 22-23 0.4709965484965366 1107 24-25 0.28419185714793826 1107 26-27 0.31181346677536936 1107 28-29 0.3374204040079647 1107 30-31 0.330383166054915 1107 32-33 0.5378852994702896 1107 34-35 0.4639878754711404 1107 36-37 0.39286323979888493 1107 38-39 0.4422586640716233 1107 40-41 0.49149451908010633 1107 42-43 0.3819886808084405 1107 44-45 0.25369476681908765 1107 46-47 0.3780548854794006 1107 48-49 0.20653630112518329 1107 50-51 0.13348183050671736 1107 52-53 0.121088483433347 1107 54-55 0.29398809514548674 1107 56-57 0.26573373452687576 1107 58-59 0.19285305645028217 1107 60-61 0.12527762761473582 1107 62-63 0.09517239584207715 1107 64-65 0.049532695391029335 1107 66-67 0.03884363997573104 1107 68-69 0.3839874182888252 1107 70-71 0.3434109956272735 1107 72-73 0.3196614972530121 1107 74-75 0.3560550037737258 1107 76-77 0.43289380119784937 1107 78-79 0.6752646052352773 1107 80-81 0.3968290040755882 1107 82-83 0.4119814823664143 1107 84-85 0.1771666288468836 1107 86-87 0.4892037671085241 1107 88-89 0.6418970905808905 1107 90-91 0.4261396633067811 1107 92-93 0.3435651644988589 1107 94-95 0.3308021419076965 1107 96-97 0.1441528230724991 1107 98-99 0.44044428821193193 1107 100-101 0.771978122200057 1108 1 0.19723901702107227 1108 2 0.35240144422107633 1108 3 0.2315077630153688 1108 4 0.13005193360975653 1108 5 0.024257270310542367 1108 6 0.17236267878542577 1108 7 0.23145910131958658 1108 8 0.2955151184176543 1108 9 0.5132668209555789 1108 10-11 0.33757138278549803 1108 12-13 0.1861099904207677 1108 14-15 0.3424796808025903 1108 16-17 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sequence quality scores pass #Quality Count 2 3054.0 3 576.0 4 505.0 5 471.0 6 568.0 7 684.0 8 959.0 9 1072.0 10 1423.0 11 1857.0 12 2683.0 13 4021.0 14 3957.0 15 4005.0 16 5175.0 17 6824.0 18 5887.0 19 3941.0 20 3799.0 21 3987.0 22 4388.0 23 4955.0 24 5706.0 25 6628.0 26 7683.0 27 8871.0 28 10322.0 29 12283.0 30 14762.0 31 17417.0 32 21528.0 33 26784.0 34 36004.0 35 56403.0 36 70955.0 37 113964.0 38 117431.0 39 17709.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.30542588703147 23.354983770402217 16.637312931664503 17.70227741090181 2 13.767425509335768 26.31822766594276 37.29428617425673 22.620060650464737 3 21.826007672025035 28.123968096204237 33.613819502057645 16.43620472971308 4 10.697303780622052 19.896981745888308 43.90499975354485 25.500714719944796 5 9.261638663332336 41.653220903752434 37.231071049492456 11.85406938342277 6 23.066586942809085 41.74118876156434 18.223362594617328 16.968861701009253 7 20.944188804904776 37.09055006218037 25.692239199754237 16.27302193316062 8 21.77887150641589 31.123351672271294 22.780969340734618 24.316807480578195 9 20.934087507659754 22.187576494863652 28.210566435737743 28.667769561738847 10-11 22.21257582166662 27.335882282884118 29.591250888093995 20.860291007355265 12-13 26.413973813474396 27.547436381856716 28.40145553711948 17.637134267549406 14-15 21.721972357423038 29.610334134269813 31.41847904216212 17.24921446614503 16-17 16.35879765576551 32.261972721784375 32.19502979576247 19.18419982668764 18-19 16.09684128898279 34.090289255519444 32.604499019166504 17.208370436331254 20-21 18.745913086338618 29.778608395629675 30.9316520167584 20.54382650127331 22-23 17.455239184446107 29.583382629385707 32.71705051859447 20.244327667573714 24-25 20.870419794638515 30.440534570936485 31.101928057743127 17.587117576681873 26-27 19.120656077177816 27.80948780948781 36.12377047159656 16.946085641737817 28-29 16.22789286688556 32.888561314940986 33.3982455621399 17.48530025603355 30-31 17.743564126222246 29.597419488416538 32.41570490410583 20.243311481255386 32-33 18.30609734957497 30.449531342810264 33.37049509489394 17.873876212720823 34-35 20.510342861897197 28.853582046396454 32.36523717614457 18.27083791556178 36-37 19.11366669885915 28.12013243491547 35.13000397040603 17.636196895819353 38-39 17.87201029991912 30.891091560338708 33.624449102883645 17.612449036858525 40-41 17.21579870062906 31.351634526142107 33.49563782613179 17.93692894709704 42-43 16.81377589334196 31.306290322314446 30.795149026487913 21.084784757855683 44-45 18.427603602651804 28.70656810438182 31.76850849158765 21.097319801378728 46-47 20.69435939251187 31.16496296870951 31.60848014076347 16.53219749801515 48-49 18.908854669697856 30.02519386564042 32.37931791653666 18.68663354812507 50-51 15.184048573371808 30.970972193791642 35.336781320778385 18.50819791205816 52-53 17.804993206689964 32.86071815935141 30.468862207239706 18.865426426718923 54-55 16.634271513476797 29.587545734940797 32.25863960165104 21.519543149931373 56-57 19.37940105253951 29.34428339684106 30.96723727356291 20.309078277056518 58-59 21.49174990470187 28.753879995313476 31.08823039210024 18.666139707884412 60-61 18.64265886858551 30.46339866370445 33.56308644357689 17.330856024133148 62-63 17.12512306977489 30.349728606903792 33.404747501673256 19.12040082164807 64-65 17.114847210240196 32.69036287309967 31.474153908388057 18.72063600827207 66-67 18.476942524123388 29.670260715061236 29.5599540609114 22.29284269990398 68-69 16.954130470502655 29.443097194389605 32.070193516851084 21.532578818256656 70-71 20.44630966407057 29.783260415281994 30.313488449665076 19.456941470982358 72-73 19.282253909965128 27.152957571594634 34.05260950544225 19.512179012997994 74-75 17.041678534130828 32.70664866939928 30.826429565052276 19.425243231417618 76-77 18.230577607852656 29.12471502599227 31.002530717763065 21.642176648392013 78-79 18.688129841922674 29.288288942509233 32.025924813419905 19.997656402148188 80-81 20.303945247936444 28.850538385939668 30.698437034549286 20.14707933157461 82-83 18.79136305902801 28.27068885406118 33.2285200948436 19.709427992067205 84-85 18.313269917256463 29.325629861956543 31.963270948971378 20.397829271815613 86-87 18.345451394333534 29.97779960220849 31.401843705898376 20.274905297559606 88-89 18.697298653618855 29.376646659139084 29.81608592011516 22.109968767126905 90-91 19.47362685805707 27.015320148000654 31.196784403465237 22.31426859047704 92-93 21.00623738384885 29.02988247629505 31.377136747726514 18.586743392129584 94-95 19.950613247736236 27.62752052415014 31.58721195189126 20.834654276222366 96-97 17.859575286610955 27.790138123074914 33.17405686896264 21.176229721351493 98-99 19.65521454766414 29.001458002750883 29.89277157571625 21.450555873868726 100-101 19.242137479684928 26.47417870988883 31.463122524326497 22.820561286099746 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11130.0 1 6399.0 2 1616.5 3 1364.5 4 987.5 5 1012.0 6 1709.0 7 2914.5 8 3872.0 9 3851.5 10 2882.5 11 2005.0 12 1790.0 13 1695.5 14 1495.0 15 1378.0 16 1515.5 17 1504.5 18 1337.5 19 1287.0 20 1434.0 21 1674.5 22 1842.0 23 2027.5 24 2210.0 25 2714.5 26 4238.5 27 5802.5 28 6001.0 29 6397.0 30 8120.0 31 10025.5 32 12815.0 33 17069.5 34 21204.5 35 23365.0 36 26307.0 37 29516.5 38 28131.0 39 25069.5 40 22663.0 41 20348.0 42 18182.5 43 16814.0 44 16115.0 45 15845.5 46 18407.5 47 30947.0 48 56643.0 49 55013.0 50 26969.0 51 11395.0 52 5315.0 53 3251.0 54 2246.0 55 1549.0 56 988.5 57 639.5 58 517.0 59 451.0 60 408.0 61 391.0 62 351.5 63 277.5 64 223.0 65 208.5 66 166.5 67 122.5 68 112.5 69 99.5 70 84.5 71 71.0 72 61.0 73 49.5 74 37.5 75 30.0 76 22.0 77 23.0 78 17.5 79 7.5 80 8.5 81 8.0 82 3.5 83 3.0 84 2.5 85 1.5 86 1.0 87 1.0 88 0.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28067664409216697 2 0.08338230128586013 3 0.08814231454824192 4 0.10045269367509133 5 0.10242235433538724 6 0.07780159608168839 7 0.08682920744137797 8 0.08518782355779805 9 0.08879886810167388 10-11 0.08116643304302723 12-13 0.08732162260645196 14-15 0.09511819605345659 16-17 0.08444920081018709 18-19 0.09454371169420361 20-21 0.0971699259079315 22-23 0.11062927375328688 24-25 0.12376034482192626 26-27 0.1275355277541601 28-29 0.11949274672461846 30-31 0.11169617327761383 32-33 0.10529477613165211 34-35 0.3738251794853277 36-37 0.5762078123307323 38-39 0.56004018107747 40-41 0.5218780057842368 42-43 0.5487146322807686 44-45 0.5689036540488016 46-47 0.5585629355822481 48-49 0.580885756398935 50-51 0.5708733147090975 52-53 0.5743202208646153 54-55 0.5648822635340308 56-57 0.538209775425857 58-59 0.5332856237751172 60-61 0.48240272338413964 62-63 0.5558546521743413 64-65 0.5891747450110136 66-67 0.6003361554193571 68-69 0.6052603070700969 70-71 0.5996796018659252 72-73 0.5925395819723525 74-75 0.5899954369528037 76-77 0.5720222834276035 78-79 0.5475656635622627 80-81 0.5433801346591338 82-83 0.5584808663880692 84-85 0.5670160625826848 86-87 0.5575781052521002 88-89 0.5695602076022336 90-91 0.5858099080496748 92-93 0.5940988966617534 94-95 0.5931961355257845 96-97 0.5886823298459397 98-99 0.5940168274675744 100-101 0.5699705535731286 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 609242.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.538390148591834 #Duplication Level Percentage of deduplicated Percentage of total 1 72.95310699702317 12.06526946068734 2 9.449292300909336 3.125521654010472 3 3.8465186803996194 1.9084567995088655 4 2.2163847017024865 1.466217396645042 5 1.4785772571749936 1.2226643771997423 6 1.1016845177676802 1.0932053025303086 7 0.8317617933420562 0.9629200734288336 8 0.7407878462048586 0.9801150734296795 9 0.5388456802636571 0.8020476081075357 >10 5.147525802719874 18.491405182020248 >50 0.9807191323496003 11.344898108696016 >100 0.6558121822847479 19.72465051470386 >500 0.02599255600518818 2.786416891234737 >1k 0.02199370123515923 9.420049983866324 >5k 0.00899742323256514 9.434764936890705 >10k+ 0.0019994273850144754 5.171396637040296 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 19091 3.1335659721424323 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12187 2.0003545389188533 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8329 1.3671086366337186 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8237 1.3520079049047833 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8189 1.3441292622635996 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6093 1.0000952002652477 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5485 0.9002990601435883 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5272 0.8653375834233359 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5260 0.8633679227630399 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5125 0.841209240334711 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5074 0.8328381825284534 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4887 0.8021443039055088 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4570 0.7501124347960252 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4317 0.7085854225414532 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4287 0.7036612708907134 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3607 0.5920471668072785 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3367 0.5526539536013604 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3315 0.5441187574067448 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2931 0.48108961627727576 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2930 0.4809254778889177 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2925 0.48010478594712774 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2746 0.4507240144310471 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2320 0.38080106099054234 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1916 0.3144891520939134 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1863 0.3057898175109398 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1721 0.2824821663641049 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1636 0.2685304033536755 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1619 0.2657400507515897 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1380 0.2265109759340295 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1306 0.21436473519553806 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1183 0.194175713427505 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1107 0.18170119591229758 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1042 0.17103220066902808 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 989 0.16233286608605446 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 963 0.15806526798874668 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 890 0.14608316563861323 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 878 0.14411350497831732 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 858 0.14083073721115746 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 764 0.1254017287055062 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 752 0.12343206804521027 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 689 0.11309134957865676 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 668 0.10964444342313892 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 661 0.10849547470463297 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 627 0.10291476950046122 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 614 0.10078097045180734 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.20691941789962E-5 0.0 12-13 0.0 0.0 0.0 3.282767767159848E-4 0.0 14-15 0.0 0.0 0.0 4.1034597089498095E-4 0.0 16-17 0.0 0.0 0.0 0.0010668995243269504 0.0 18-19 0.0 0.0 0.0 0.0015593146894009279 0.0 20-21 0.0 0.0 0.0 0.0022979374370118936 0.0 22-23 0.0 0.0 0.0 0.003775182932233825 0.0 24-25 0.0 0.0 0.0 0.007796573447004639 0.0 26-27 0.0 0.0 0.0 0.013869693816250358 0.0 28-29 0.0 0.0 0.0 0.0409525278953191 0.0 30-31 0.0 0.0 0.0 0.09667751074285752 0.0 32-33 0.0 0.0 0.0 0.20344953236973157 0.0 34-35 0.0 0.0 0.0 0.35224098141625165 0.0 36-37 0.0 0.0 0.0 0.5432159962707758 0.0 38-39 0.0 0.0 0.0 0.7994360204976019 0.0 40-41 0.0 0.0 0.0 1.0710029840359003 0.0 42-43 0.0 0.0 0.0 1.3490534139143395 0.0 44-45 0.0 0.0 0.0 1.6852088332715078 0.0 46-47 0.0 0.0 0.0 2.0843933937581456 0.0 48-49 0.0 0.0 0.0 2.422846750552326 0.0 50-51 0.0 0.0 0.0 2.7751698011627566 0.0 52-53 0.0 0.0 0.0 3.1051700309565 0.0 54-55 0.0 0.0 0.0 3.484822123228536 0.0 56-57 0.0 0.0 0.0 4.020077407663949 0.0 58-59 0.0 0.0 0.0 4.4736738438912615 0.0 60-61 0.0 0.0 0.0 4.990053213665506 0.0 62-63 0.0 0.0 0.0 5.498800148381103 0.0 64-65 0.0 0.0 0.0 6.020842292553698 0.0 66-67 0.0 0.0 0.0 6.637674356003034 0.0 68-69 0.0 0.0 0.0 7.337150097990618 0.0 70-71 0.0 0.0 0.0 8.073967323329645 0.0 72-73 0.0 0.0 0.0 8.751858867248155 0.0 74-75 0.0 0.0 0.0 9.572140463067221 0.0 76-77 0.0 0.0 0.0 10.310681141484007 0.0 78-79 0.0 0.0 0.0 11.331375709488185 0.0 80-81 0.0 0.0 0.0 12.368237908745622 0.0 82-83 0.0 0.0 0.0 13.257950042840118 0.0 84-85 0.0 8.20691941789962E-5 0.0 14.245406587201803 0.0 86-87 0.0 1.641383883579924E-4 0.0 15.231714162844977 0.0 88-89 0.0 1.641383883579924E-4 0.0 16.317407532638917 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCCTC 45 9.781842E-4 42.080658 6 GCTTTGC 435 0.0 37.07506 1 ATGTATT 65 1.338306E-4 36.4879 1 CTTTGCG 480 0.0 33.53027 2 GTAAGCG 95 0.0 32.543434 94-95 GGCGGCG 30 0.0039252923 31.70898 94-95 GGATGAC 105 1.9501767E-6 31.560493 8 CCGCGGA 40 4.4587767E-4 29.72717 86-87 GTACCCA 80 4.5277423E-4 29.646418 1 CTATACA 160 1.9736035E-9 29.585533 4 ACAATCA 145 2.4756446E-8 29.38391 8 GTATGGT 65 0.0058815954 29.19032 1 GGTATGG 65 0.005938599 29.132763 8 TGGGATA 65 0.005938599 29.132763 6 TTGCGTT 555 0.0 28.999153 4 TTTGCGT 560 0.0 28.740232 3 GATGACC 100 5.063936E-5 28.404446 9 TACAATC 150 3.44171E-8 28.404444 7 GCGTAAA 85 6.455733E-4 27.902514 1 CGTGTCA 85 6.530677E-4 27.847494 9 >>END_MODULE