##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939058_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 609242 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.016655122266684 33.0 31.0 34.0 30.0 34.0 2 32.2442871633932 34.0 31.0 34.0 30.0 34.0 3 32.32336903890408 34.0 31.0 34.0 30.0 34.0 4 35.90064046799137 37.0 35.0 37.0 35.0 37.0 5 35.824790477347264 37.0 35.0 37.0 35.0 37.0 6 35.913098571667746 37.0 35.0 37.0 35.0 37.0 7 35.8743274429537 37.0 35.0 37.0 35.0 37.0 8 35.87661060793576 37.0 35.0 37.0 35.0 37.0 9 37.477398143923104 39.0 37.0 39.0 34.0 39.0 10-11 37.40328555811976 39.0 37.0 39.0 34.0 39.0 12-13 37.383548737611655 39.0 37.0 39.0 34.0 39.0 14-15 38.46661096904022 40.0 38.0 41.0 34.0 41.0 16-17 38.464409873252336 40.0 38.0 41.0 33.5 41.0 18-19 38.57177361376924 40.0 38.0 41.0 34.0 41.0 20-21 38.55700115881702 40.0 38.0 41.0 34.0 41.0 22-23 38.48701747417282 40.0 38.0 41.0 34.0 41.0 24-25 38.362529996290476 40.0 38.0 41.0 34.0 41.0 26-27 38.135678433200596 40.0 37.0 41.0 33.5 41.0 28-29 37.983269374074666 40.0 37.0 41.0 33.0 41.0 30-31 37.856559462413955 40.0 37.0 41.0 33.0 41.0 32-33 37.60431404926121 40.0 36.0 41.0 33.0 41.0 34-35 37.46542588987627 40.0 36.0 41.0 33.0 41.0 36-37 37.31099464580577 40.0 35.5 41.0 32.5 41.0 38-39 37.134098601212656 39.0 35.0 41.0 32.0 41.0 40-41 37.09415634509767 40.0 35.0 41.0 32.0 41.0 42-43 36.89035145311715 40.0 35.0 41.0 31.0 41.0 44-45 36.62834308862488 39.0 35.0 41.0 31.0 41.0 46-47 36.30029200219289 39.0 35.0 41.0 30.0 41.0 48-49 35.95559482110557 39.0 35.0 41.0 29.0 41.0 50-51 35.70013968176849 39.0 35.0 41.0 28.0 41.0 52-53 35.60558694246293 39.0 35.0 40.5 28.0 41.0 54-55 35.43934101719842 39.0 35.0 40.0 27.0 41.0 56-57 35.121809560076294 38.5 35.0 40.0 26.5 41.0 58-59 34.85796694909412 38.0 34.5 40.0 26.0 41.0 60-61 34.329612042505275 38.0 34.0 40.0 24.0 41.0 62-63 34.10591521923965 38.0 34.0 40.0 24.0 41.0 64-65 33.896720843277386 37.0 33.5 39.5 23.5 41.0 66-67 33.35989885792509 37.0 33.0 39.0 22.0 40.5 68-69 32.856396144717536 36.0 32.5 39.0 21.5 40.0 70-71 32.28046818833895 35.5 32.0 38.5 20.0 40.0 72-73 31.694554873104615 35.0 31.0 37.0 18.0 39.0 74-75 31.233757685780034 35.0 31.0 37.0 14.5 39.0 76-77 29.22019493075001 32.5 28.5 35.0 8.0 37.0 78-79 30.19874532615939 34.0 30.0 36.0 7.5 37.5 80-81 30.114683491945726 34.0 30.0 35.5 4.5 37.0 82-83 29.73424271471763 34.0 30.0 35.0 2.0 37.0 84-85 29.302623587999513 34.0 29.0 35.0 2.0 36.0 86-87 28.84064952843041 34.0 29.0 35.0 2.0 36.0 88-89 28.219917536873687 34.0 28.5 35.0 2.0 35.0 90-91 27.71746038519997 33.0 27.0 35.0 2.0 35.0 92-93 27.39225299634628 33.0 27.0 35.0 2.0 35.0 94-95 26.95844180145164 33.0 26.0 35.0 2.0 35.0 96-97 26.286439542907416 33.0 25.0 34.5 2.0 35.0 98-99 25.463378263481506 32.0 22.0 34.0 2.0 35.0 100-101 21.17931790651334 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.034298066157390394 1101 2 0.0769349480458601 1101 3 0.15354810156503618 1101 4 0.11700724795201722 1101 5 0.16063387423233166 1101 6 0.11402118968791797 1101 7 0.15435520935933766 1101 8 0.18729113900306515 1101 9 0.2636134275183508 1101 10-11 0.10713279139679344 1101 12-13 0.19012421670809942 1101 14-15 0.3828123380849391 1101 16-17 0.1654060975275442 1101 18-19 0.26591061071246 1101 20-21 0.40844575316539533 1101 22-23 0.3868228416209192 1101 24-25 0.4075894999206042 1101 26-27 0.4701301937802711 1101 28-29 0.45843296979668935 1101 30-31 0.2904476406066223 1101 32-33 0.4234364264850967 1101 34-35 0.4575185190314457 1101 36-37 0.387841310898537 1101 38-39 0.35706809957950725 1101 40-41 0.26887348714348036 1101 42-43 0.30979980075687763 1101 44-45 0.4522685717566617 1101 46-47 0.6176710820147449 1101 48-49 0.5925090005837603 1101 50-51 0.5780135743245225 1101 52-53 0.8238932242133785 1101 54-55 0.81065359176052 1101 56-57 0.4852325178649224 1101 58-59 0.8633108265350202 1101 60-61 0.9099385038826284 1101 62-63 0.8077228257484421 1101 64-65 0.8889581088330303 1101 66-67 0.8217759255450332 1101 68-69 0.9805922985214508 1101 70-71 0.9481784891068301 1101 72-73 1.040005535628918 1101 74-75 0.9103868209754999 1101 76-77 0.4519740686314613 1101 78-79 0.7743232032425134 1101 80-81 1.1177142127573028 1101 82-83 1.1372069885650937 1101 84-85 0.8559619033117336 1101 86-87 0.8942244406853526 1101 88-89 1.0101860840978851 1101 90-91 1.1804406630505397 1101 92-93 1.389066457453957 1101 94-95 1.5882651633726717 1101 96-97 1.261732222381486 1101 98-99 1.4813436124897734 1101 100-101 1.688714151019429 1102 1 0.027460961766948344 1102 2 0.17340840794017254 1102 3 0.19862187200445902 1102 4 0.0633807072073509 1102 5 -0.030054245180572536 1102 6 0.19976295161610835 1102 7 0.08071256627535917 1102 8 0.15361999733442389 1102 9 0.24082733942832846 1102 10-11 0.1250817878760131 1102 12-13 0.16047546495887843 1102 14-15 0.22020267284745643 1102 16-17 0.37605121347077386 1102 18-19 0.21633361661026385 1102 20-21 0.27462700671973295 1102 22-23 0.3652971449711018 1102 24-25 0.34066574960910145 1102 26-27 0.3224503364009834 1102 28-29 0.42308609857067836 1102 30-31 0.44635438761459767 1102 32-33 0.42584858247332136 1102 34-35 0.48598355714670305 1102 36-37 0.6090471971981302 1102 38-39 0.7160272622578674 1102 40-41 0.8954669478045716 1102 42-43 0.8794264437386161 1102 44-45 0.6311738682761288 1102 46-47 0.6315657394886003 1102 48-49 0.8957952835632028 1102 50-51 0.7169521617915322 1102 52-53 0.6488984958125528 1102 54-55 0.7491452115152768 1102 56-57 0.5506386865944464 1102 58-59 0.6609676805724192 1102 60-61 0.7950719552145884 1102 62-63 0.7378613569850643 1102 64-65 0.7858887601470954 1102 66-67 0.6767797913844333 1102 68-69 0.8936748102307988 1102 70-71 0.7788850750914023 1102 72-73 0.8626162232371506 1102 74-75 1.097389613325614 1102 76-77 0.49409095116745405 1102 78-79 0.6148557306069051 1102 80-81 0.8499585082480081 1102 82-83 0.8472243049696679 1102 84-85 0.8010094755006953 1102 86-87 0.9170121262296682 1102 88-89 0.9383469669150273 1102 90-91 1.0391394406785146 1102 92-93 1.1019096705096345 1102 94-95 1.1629094103007667 1102 96-97 0.9259521215501678 1102 98-99 1.2249314309422878 1102 100-101 1.7365211657607347 1103 1 0.11501770690906454 1103 2 0.151784705082882 1103 3 0.11139426421198095 1103 4 0.0863675355032214 1103 5 0.16254389053579388 1103 6 0.12354902475912155 1103 7 0.06500712557286903 1103 8 0.1445811404967543 1103 9 0.20529913608354633 1103 10-11 0.23293326619952381 1103 12-13 0.03005944776921865 1103 14-15 0.14452747552029876 1103 16-17 -0.04845057815423104 1103 18-19 0.146204073117552 1103 20-21 0.034664143348990706 1103 22-23 0.12780746932664755 1103 24-25 0.08724862080461548 1103 26-27 0.11871897457967151 1103 28-29 0.25475563225136 1103 30-31 0.2653552384035933 1103 32-33 0.20765225514932695 1103 34-35 0.10734674468668715 1103 36-37 0.02502402096060763 1103 38-39 0.17621561576380174 1103 40-41 0.11143538787983687 1103 42-43 0.36023090510224876 1103 44-45 0.4933212100262665 1103 46-47 0.23357347732138578 1103 48-49 0.004373348111386122 1103 50-51 -0.06410784677846237 1103 52-53 -0.04439828050445982 1103 54-55 -0.23822026135572827 1103 56-57 -0.1282164122399152 1103 58-59 0.13160614492932865 1103 60-61 0.016572076312293404 1103 62-63 0.018731956325545696 1103 64-65 0.26973463692552 1103 66-67 0.4961594606324482 1103 68-69 0.35511916428321655 1103 70-71 0.567332043612204 1103 72-73 0.40502681276561603 1103 74-75 0.42221557418931965 1103 76-77 0.5391594800477044 1103 78-79 0.25267423309406567 1103 80-81 0.3947460283533317 1103 82-83 0.5916367326991825 1103 84-85 0.0952955269082203 1103 86-87 0.09097518832484397 1103 88-89 0.6022402891529133 1103 90-91 0.5065020568857328 1103 92-93 0.14883503085292915 1103 94-95 0.3234927286262632 1103 96-97 0.21795439446092146 1103 98-99 0.47754900439270287 1103 100-101 0.6985073544123281 1104 1 -0.10980269206728366 1104 2 -0.18929074276344693 1104 3 -0.012214405969622533 1104 4 -0.001345596183639941 1104 5 0.08711146060331743 1104 6 -0.055478508925702386 1104 7 -0.020893037840941986 1104 8 0.05153085221013498 1104 9 -0.11698680724577315 1104 10-11 -0.019965016413870273 1104 12-13 -0.14702561990876006 1104 14-15 -0.18373694651898376 1104 16-17 0.015720173857609154 1104 18-19 -0.134903132506345 1104 20-21 -0.011136430140972209 1104 22-23 -0.1706832654188304 1104 24-25 -0.07060757504528681 1104 26-27 -0.2668435697424698 1104 28-29 -0.11148708350111036 1104 30-31 -0.06736143905455094 1104 32-33 0.03162937721636183 1104 34-35 -0.1884476622445348 1104 36-37 -0.34138365023847683 1104 38-39 0.06069901230436159 1104 40-41 -0.0021680407268220847 1104 42-43 -0.08150282289084743 1104 44-45 -0.2780382078505852 1104 46-47 -0.25429998489302363 1104 48-49 -0.01933707028962317 1104 50-51 -0.14514876189577564 1104 52-53 -0.16130235968307005 1104 54-55 -0.15450614301946786 1104 56-57 -0.4134397958307048 1104 58-59 -0.1833755095583811 1104 60-61 -0.19987876079806455 1104 62-63 -0.04561145933134725 1104 64-65 -0.31485204947413337 1104 66-67 -0.18877101141762154 1104 68-69 -0.1348355115099551 1104 70-71 -0.46146270247973575 1104 72-73 -0.5636840681273014 1104 74-75 -0.26500577443708195 1104 76-77 -0.4737008782293586 1104 78-79 -0.09469110467918895 1104 80-81 -0.18604359949484817 1104 82-83 0.10060599240380341 1104 84-85 -0.10027996718729781 1104 86-87 -0.08799376305184126 1104 88-89 0.07520147066557215 1104 90-91 -0.0037159309661483064 1104 92-93 0.09857757702503633 1104 94-95 -0.3683843718578075 1104 96-97 -0.14182730935926102 1104 98-99 0.16876628309032427 1104 100-101 0.8590244203636743 1105 1 0.18551109575798108 1105 2 0.21025594274226478 1105 3 0.24337843519368363 1105 4 0.17041938674347534 1105 5 0.14424674848746122 1105 6 0.2506736163539358 1105 7 0.2646656575552697 1105 8 0.12605104531033362 1105 9 0.2221100960863822 1105 10-11 0.14383163965583634 1105 12-13 0.2501279182991709 1105 14-15 0.3853169802442338 1105 16-17 0.3963277330239805 1105 18-19 0.16341901514466173 1105 20-21 0.2060218178279456 1105 22-23 0.17684705366747977 1105 24-25 0.3055257111904339 1105 26-27 0.03431032169753934 1105 28-29 0.2833204050188485 1105 30-31 0.16215110068405636 1105 32-33 0.027296397115101456 1105 34-35 0.22349452543944182 1105 36-37 0.3236581488902104 1105 38-39 0.27051003314505095 1105 40-41 0.36167571295267464 1105 42-43 0.4008650094416595 1105 44-45 0.3713053692128838 1105 46-47 0.6180154513485903 1105 48-49 0.6521865373872942 1105 50-51 0.30632892561158087 1105 52-53 0.2883973930544741 1105 54-55 0.3009748413205813 1105 56-57 0.5754952094677748 1105 58-59 0.5845127513623751 1105 60-61 0.6135882120030729 1105 62-63 0.539293756728938 1105 64-65 0.22730325489417424 1105 66-67 0.3768977746414848 1105 68-69 0.39806805613398666 1105 70-71 0.5477831470433969 1105 72-73 0.598885106487085 1105 74-75 0.6347381323096535 1105 76-77 0.6819096245376244 1105 78-79 0.7720491520027828 1105 80-81 0.6936514434817234 1105 82-83 0.8643496636624839 1105 84-85 1.046794093804106 1105 86-87 0.7850487425410009 1105 88-89 0.9216573755283974 1105 90-91 1.0362758269521386 1105 92-93 1.2688648724447944 1105 94-95 1.0340823654552693 1105 96-97 1.015705364607534 1105 98-99 1.3623374593531317 1105 100-101 1.557328906411005 1106 1 -0.19590937291080834 1106 2 -0.1955640016682807 1106 3 -0.23780363155884743 1106 4 -0.09025220614287832 1106 5 -0.05279638983084567 1106 6 -0.24032699377418965 1106 7 -0.2768738990849542 1106 8 -0.2206825094769158 1106 9 -0.4993720633152421 1106 10-11 -0.2296938840374736 1106 12-13 -0.13233485258641764 1106 14-15 -0.06551968619645976 1106 16-17 -0.1739838842674999 1106 18-19 -0.13877518637840325 1106 20-21 -0.22401964828734577 1106 22-23 -0.04473961842255392 1106 24-25 0.15467153181276672 1106 26-27 0.0357791486697252 1106 28-29 -0.09450143756809837 1106 30-31 -0.09106337065122005 1106 32-33 -0.18851947977459815 1106 34-35 0.047464086825115714 1106 36-37 -0.1160691012397237 1106 38-39 0.18444524657691375 1106 40-41 -0.07433709973799552 1106 42-43 -0.005435128402105249 1106 44-45 0.09118029294686636 1106 46-47 -0.05800368859672744 1106 48-49 -0.12083450073442492 1106 50-51 0.3486311424104471 1106 52-53 0.5803775977863594 1106 54-55 0.3904433519300312 1106 56-57 0.10926975582621168 1106 58-59 0.4876859825557389 1106 60-61 0.5040730378905849 1106 62-63 0.3042644931761842 1106 64-65 0.20599678979575486 1106 66-67 0.331581637882401 1106 68-69 0.22910211032240824 1106 70-71 0.271330138207837 1106 72-73 0.18763615161396885 1106 74-75 0.2683364194314315 1106 76-77 -0.13636258299632686 1106 78-79 0.37648557439222685 1106 80-81 0.2840520942850553 1106 82-83 0.43054574076460383 1106 84-85 0.24436826956620195 1106 86-87 0.22008704502896848 1106 88-89 0.4350632463168189 1106 90-91 0.6081749072404783 1106 92-93 0.50040284727136 1106 94-95 0.46953871869686026 1106 96-97 0.3822822068555176 1106 98-99 0.39816553880536887 1106 100-101 0.7656255190700385 1107 1 0.020111378824232418 1107 2 -0.007819206780631305 1107 3 0.01669715865622834 1107 4 -0.17542364845717628 1107 5 0.033405767799884245 1107 6 -0.05479823681685758 1107 7 -0.01033091825626542 1107 8 -0.09476345498642758 1107 9 -0.04190624870656734 1107 10-11 -0.03643476220692321 1107 12-13 0.02269332029889881 1107 14-15 -0.023165875985505124 1107 16-17 0.023776027778126263 1107 18-19 -0.01687592162199536 1107 20-21 0.2531671860423401 1107 22-23 0.06887097733089576 1107 24-25 0.16108773465754211 1107 26-27 0.0487489830681298 1107 28-29 0.1681763608239919 1107 30-31 -0.25219853178638374 1107 32-33 -0.043755513582070193 1107 34-35 0.01728989593663499 1107 36-37 -0.022926793811699042 1107 38-39 -0.02284019285138328 1107 40-41 0.08537223854278153 1107 42-43 -0.10571513939640198 1107 44-45 -0.11437010903210876 1107 46-47 -0.36620474679778425 1107 48-49 -0.34219779352628166 1107 50-51 -0.7370213003848605 1107 52-53 -0.4764921197976477 1107 54-55 -0.5237238300942977 1107 56-57 -0.39176954878451653 1107 58-59 -0.5295355525229368 1107 60-61 -0.6962894303290312 1107 62-63 -0.6110966007563334 1107 64-65 -0.7524997401293447 1107 66-67 -0.7662504242353805 1107 68-69 -0.8867331126556763 1107 70-71 -0.7283262900200143 1107 72-73 -0.5586446839525827 1107 74-75 -0.44191232653160384 1107 76-77 -0.9681154861778047 1107 78-79 -0.5610444881378349 1107 80-81 -0.46656633490480104 1107 82-83 -0.35881040573439904 1107 84-85 -0.370831345633416 1107 86-87 -0.3311910419634039 1107 88-89 -0.2512127792449199 1107 90-91 -0.17999591664462855 1107 92-93 -0.0991041272356128 1107 94-95 0.06834137107094307 1107 96-97 0.0828594074327178 1107 98-99 0.17632983482681297 1107 100-101 0.6022485521216403 1108 1 0.004615332690043772 1108 2 0.00104677616076998 1108 3 0.058851583878087865 1108 4 0.027276721030716544 1108 5 0.11022126557259782 1108 6 0.1303082931047541 1108 7 0.18144020643727288 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-1.5039859593886789 2316 60-61 -1.188061752336857 2316 62-63 -1.2544264122449604 2316 64-65 -1.1585730686433138 2316 66-67 -1.023882632432013 2316 68-69 -1.1908444029483611 2316 70-71 -1.0711500680325834 2316 72-73 -1.078976386115734 2316 74-75 -1.4247433335938275 2316 76-77 -1.058955909540611 2316 78-79 -1.1719988153728167 2316 80-81 -1.043625223855475 2316 82-83 -1.6126427288543823 2316 84-85 -1.9202020475926709 2316 86-87 -1.5534796849410313 2316 88-89 -1.8398590006762738 2316 90-91 -2.037097067731768 2316 92-93 -2.782654795459308 2316 94-95 -2.4292883347623473 2316 96-97 -2.6369083361769867 2316 98-99 -2.7520353804691204 2316 100-101 -3.436192846236068 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 19.0 5 53.0 6 172.0 7 245.0 8 312.0 9 521.0 10 843.0 11 1210.0 12 1678.0 13 2285.0 14 2806.0 15 3779.0 16 4825.0 17 6680.0 18 6362.0 19 4220.0 20 3831.0 21 4045.0 22 4458.0 23 4926.0 24 5703.0 25 6607.0 26 7579.0 27 8985.0 28 10838.0 29 12832.0 30 15553.0 31 18557.0 32 22739.0 33 29145.0 34 39911.0 35 58663.0 36 79539.0 37 130313.0 38 102369.0 39 6638.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.04885677841244 24.279244686005466 17.066582728240558 17.605315807341537 2 13.890046976406747 26.816273336375364 36.94623811227722 22.347441574940664 3 21.69121629828541 28.273329809829264 33.61521365894012 16.4202402329452 4 10.797351462965455 20.562764878324213 43.00589913367759 25.633984525032744 5 9.151073468583808 42.086862320267876 37.08144573567067 11.680618475477644 6 23.0721125595412 42.23691078422039 17.794406820278315 16.896569835960097 7 20.982630875743958 37.267292799905455 25.074929174285423 16.67514715006516 8 22.022119289215123 31.862379809665125 22.267013764645245 23.848487136474507 9 20.56145177121735 22.368615427038847 27.817845782135837 29.25208701960797 10-11 22.306406977851164 27.695070267644056 29.27350707928869 20.72501567521609 12-13 26.28175667468756 27.686042656284364 28.19757994360205 17.83462072542602 14-15 21.443713381300633 30.06174141484108 30.96426745366521 17.530277750193072 16-17 16.432243535239795 32.600627681681345 31.725911238194037 19.24121754488482 18-19 16.15359742105764 34.26011338679868 32.21010698540153 17.37618220674215 20-21 18.658267158206428 29.998588409860123 30.65095643438896 20.69218799754449 22-23 17.443889291939822 29.9740497208006 32.321885227873324 20.260175759386254 24-25 20.85722914703845 30.872953604643143 30.767987105288206 17.50183014303019 26-27 19.206325236933765 28.26774910462509 35.705598103873335 16.820327554567807 28-29 16.329389634989052 33.26001818653343 33.00191057084049 17.408681607637032 30-31 17.784312309394295 29.9920228743258 32.13673712580551 20.086927690474393 32-33 18.344762836442662 30.745664284471523 33.098588081583344 17.81098479750247 34-35 20.51460504296856 29.14180240617375 32.24993413757982 18.093658413277865 36-37 19.020772511898908 28.514866762585676 34.71871463054903 17.745646094966386 38-39 17.68244936955098 31.184121319371144 33.502033097547645 17.631396213530234 40-41 17.12707363693739 31.501389879445295 33.30367819469146 18.06785828892586 42-43 16.817715886307248 31.57408798032298 30.63956069749367 20.9686354358761 44-45 18.489900498655697 28.869259632524564 31.522081682363872 21.118758186455867 46-47 20.582019744255348 31.404921373414936 31.35280730680249 16.660251575527234 48-49 18.97081778803462 30.19289575061429 32.08278825620731 18.753498205143774 50-51 15.104486823326457 31.15162427912176 34.99658180403601 18.74730709351577 52-53 17.691163774001136 33.195101453937845 30.19038411665644 18.923350655404587 54-55 16.555490264952187 29.94704895591571 31.72491390941531 21.7725468697168 56-57 19.345760797843877 29.715449689942584 30.533925763489712 20.404863748723823 58-59 21.376762233664508 29.185330599877553 30.630981828209197 18.80692533824874 60-61 18.428963845256746 31.2622439692168 32.82577883339338 17.483013352133067 62-63 16.90510503215471 31.003115346611033 32.799692076383444 19.292087544850816 64-65 16.761689349279965 33.31935417950711 30.968065977229024 18.950890493983902 66-67 18.254248986636487 30.216000925753427 28.95055836555278 22.579191722057306 68-69 16.79223418259651 30.15064819100707 31.34887416001077 21.708243466385657 70-71 20.150203039186398 30.38464189927812 29.75287324248821 19.712281819047277 72-73 19.02766060120609 27.769999441929478 33.49055055298223 19.7117894038822 74-75 16.65988227994787 33.37992127922894 30.000886347297133 19.959310093526053 76-77 17.976150564353375 29.7279797003701 30.261600962391782 22.034268772884744 78-79 18.197530702085544 29.92103302136097 31.478049773324884 20.403386503228603 80-81 19.76808887108899 29.322830664990267 30.11250045138057 20.796580012540172 82-83 18.175454088851392 29.150813633991092 32.52902787398111 20.144704403176405 84-85 17.529979876633586 30.05981202871765 31.206811086563302 21.203397008085457 86-87 17.468854636340176 30.834855191935002 30.58631691588985 21.10997325583498 88-89 17.580717608904383 30.162156370664327 29.224740075906492 23.0323859445248 90-91 18.379414423537664 27.66866074207269 30.715100798782807 23.236824035606844 92-93 19.719914254105923 30.181356505296748 30.54902649521865 19.54970274537868 94-95 18.691423112654743 28.416212276894896 31.08756454742122 21.80480006302914 96-97 16.236744723125675 29.094845450509123 32.54144312941484 22.126966696950372 98-99 18.038726811349186 30.076225867553454 29.175680599827324 22.709366721270037 100-101 17.288478615194972 27.91916499382018 30.995862064437553 23.796494326547293 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10389.0 1 6129.0 2 1662.5 3 1330.5 4 1033.5 5 997.5 6 1645.5 7 2813.0 8 3559.0 9 3455.5 10 2626.0 11 1843.5 12 1640.5 13 1466.0 14 1342.5 15 1227.0 16 1150.0 17 1177.5 18 1172.0 19 1239.5 20 1389.0 21 1614.5 22 1800.0 23 1965.5 24 2126.5 25 2666.0 26 4204.0 27 5819.5 28 6006.0 29 6304.0 30 8146.5 31 10015.0 32 12872.0 33 17369.0 34 21421.5 35 23422.0 36 26291.0 37 29553.0 38 28476.0 39 25564.5 40 23046.0 41 20697.0 42 18506.5 43 17340.0 44 16711.5 45 16387.0 46 18051.5 47 31561.5 48 58494.0 49 56553.5 50 28951.5 51 12587.5 52 4728.5 53 1668.5 54 854.5 55 597.5 56 455.0 57 331.5 58 272.5 59 254.5 60 252.0 61 249.0 62 204.0 63 148.0 64 117.5 65 103.0 66 80.0 67 77.5 68 63.5 69 33.0 70 21.0 71 21.5 72 24.5 73 18.0 74 14.0 75 9.0 76 8.0 77 8.5 78 5.5 79 2.5 80 0.5 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4018107747003653 2 0.0 3 0.0 4 0.0 5 3.282767767159848E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001231037912684943 16-17 0.0026262142137278783 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0028724217962648665 36-37 0.007796573447004639 38-39 0.011982102350133444 40-41 0.002708283407906874 42-43 5.744843592529734E-4 44-45 6.565535534319696E-4 46-47 2.4620758253698856E-4 48-49 1.641383883579924E-4 50-51 4.10345970894981E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.641383883579924E-4 60-61 0.002215868242832897 62-63 0.0 64-65 2.4620758253698856E-4 66-67 0.0013951763010429353 68-69 0.0013131071068639391 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12441689837535824 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14526247369682327 88-89 0.42257428082765147 90-91 0.3189208885795792 92-93 0.0 94-95 0.0 96-97 5.744843592529734E-4 98-99 0.0 100-101 1.641383883579924E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 609242.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.196752686124725 #Duplication Level Percentage of deduplicated Percentage of total 1 70.61727061619052 10.7315319692339 2 10.234919263379597 3.1107507361606714 3 4.250148512177999 1.9376536745661002 4 2.4420802505805477 1.4844675843096833 5 1.5909704595776855 1.208879230256614 6 1.1405735270292163 1.0399808286362398 7 0.8565102338391748 0.9111321937752157 8 0.7236593400658854 0.8797817615988391 9 0.5940487119943836 0.8124850223720623 >10 5.686666306637144 18.712761103141283 >50 1.0584867959172652 11.17880907750943 >100 0.737700491440298 20.589683574014924 >500 0.03132256845061295 3.1816585199313248 >1k 0.02376194847977534 9.491958860354343 >5k 0.00972079710536264 9.575012884863487 >10k+ 0.002160177134525031 5.153452979275887 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 19937 3.2724270486932947 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11460 1.8810259305825927 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8961 1.4708440980759698 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8056 1.3222988566119869 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7873 1.292261531542474 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6008 0.9861434372548182 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5926 0.9726840894094628 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5446 0.8938976629976265 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5432 0.8915997255606147 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5419 0.8894659265119608 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5214 0.8558175568985723 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4729 0.776210438544946 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4606 0.7560214167769129 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4357 0.7151509580757729 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4306 0.7067799002695153 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3923 0.6439148975284041 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3467 0.5690677924371597 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3110 0.5104703877933563 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3110 0.5104703877933563 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3023 0.496190348006211 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2844 0.46680957649013033 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2738 0.44941090732418315 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2232 0.36635688281503903 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1984 0.32565056250225694 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1924 0.31580225920077737 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1854 0.3043125720157179 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1770 0.29052494739364654 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1656 0.27181317112083536 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1389 0.22798822142925143 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1360 0.22322820816686964 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1160 0.19040053049527117 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1159 0.19023639210691318 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1128 0.18514810206781543 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 994 0.16315355802784443 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 950 0.15593146894009277 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 922 0.15133559406606897 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 884 0.14509833530846528 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 858 0.14083073721115746 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 822 0.13492175523026975 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 793 0.13016174196788796 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 751 0.12326792965685228 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 716 0.11752308606432255 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 713 0.11703067089924857 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 711 0.11670239412253258 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 675 0.11079341214164486 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 660 0.10833133631627496 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 644 0.1057051221025471 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 629 0.1032430462771772 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 618 0.1014375240052393 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.20691941789962E-5 0.0 12-13 0.0 0.0 0.0 3.282767767159848E-4 0.0 14-15 0.0 0.0 0.0 4.1034597089498095E-4 0.0 16-17 0.0 0.0 0.0 0.0010668995243269504 0.0 18-19 0.0 0.0 0.0 0.0016413838835799238 0.0 20-21 0.0 0.0 0.0 0.0026262142137278783 0.0 22-23 0.0 0.0 0.0 0.004513805679844791 0.0 24-25 0.0 0.0 0.0 0.008699334582973596 0.0 26-27 0.0 0.0 0.0 0.014854524146398311 0.0 28-29 0.0 0.0 0.0 0.042429773390541034 0.0 30-31 0.0 0.0 0.0 0.09905751737404841 0.0 32-33 0.0 0.0 0.0 0.20771713046703938 0.0 34-35 0.0 0.0 0.0 0.35929893211564534 0.0 36-37 0.0 0.0 0.0 0.5542132682907613 0.0 38-39 0.0 0.0 0.0 0.8160139977217591 0.0 40-41 0.0 0.0 0.0 1.0919306285515444 0.0 42-43 0.0 0.0 0.0 1.3735100337796804 0.0 44-45 0.0 0.0 0.0 1.7153282275351995 0.0 46-47 0.0 0.0 0.0 2.119601078060935 0.0 48-49 0.0 0.0 0.0 2.460844787457201 0.0 50-51 0.0 0.0 0.0 2.8161223290580755 0.0 52-53 0.0 0.0 0.0 3.1501439493665897 0.0 54-55 0.0 0.0 0.0 3.532914671017428 0.0 56-57 0.0 0.0 0.0 4.071370654025822 0.0 58-59 0.0 0.0 0.0 4.529809172709695 0.0 60-61 0.0 0.0 0.0 5.046680957649013 0.0 62-63 0.0 0.0 0.0 5.559449282879381 0.0 64-65 0.0 0.0 0.0 6.084774194819136 0.0 66-67 0.0 0.0 0.0 6.704806956841452 0.0 68-69 0.0 0.0 0.0 7.406088221100974 0.0 70-71 0.0 0.0 0.0 8.146598560178058 0.0 72-73 0.0 0.0 0.0 8.827444595087009 0.0 74-75 0.0 0.0 0.0 9.653388965304426 0.0 76-77 0.0 0.0 0.0 10.395951034235985 0.0 78-79 0.0 0.0 0.0 11.418286986123741 0.0 80-81 0.0 0.0 0.0 12.459580921866845 0.0 82-83 0.0 0.0 0.0 13.35429927680626 0.0 84-85 0.0 0.0 0.0 14.344299966187492 0.0 86-87 0.0 0.0 0.0 15.32888408875291 0.0 88-89 0.0 0.0 0.0 16.410227791255362 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGGT 55 8.0130485E-7 51.977245 1 TCAGGCC 50 2.840192E-5 47.458103 3 GTCAGGC 70 7.967901E-8 47.4581 2 ATGTATT 75 1.3289718E-7 44.469418 1 GTAAGCG 70 0.0 40.67837 94-95 GGGAGCC 75 6.958946E-6 37.96648 7 CGCAGAA 300 0.0 37.96648 9 GAGCCAC 50 0.0016222371 37.96648 9 TAGGCAT 115 2.5193003E-9 37.14112 5 GCTTTGC 525 0.0 36.301567 1 CTTTGCG 540 0.0 36.033005 2 CATTCCG 120 3.8198777E-9 35.593575 9 CTCCGAC 70 4.6020432E-10 33.898643 94-95 GGCATTC 130 8.345523E-9 32.855606 7 TTTGCGT 610 0.0 31.898067 3 TCTCTAG 60 0.0038906806 31.76387 1 GTAAGGT 60 0.0039666044 31.638735 4 TTGCGTT 615 0.0 31.638733 4 CTTTAGG 155 1.36788E-9 30.61813 2 CCTTTAG 130 2.9525836E-7 29.320494 1 >>END_MODULE