##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939057_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 94682 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.20479077332545 33.0 31.0 34.0 30.0 34.0 2 31.14303669124015 33.0 31.0 34.0 27.0 34.0 3 31.094801546228428 33.0 31.0 34.0 27.0 34.0 4 34.54528843919647 37.0 35.0 37.0 32.0 37.0 5 34.630996387909 37.0 35.0 37.0 32.0 37.0 6 34.854998838216346 37.0 35.0 37.0 32.0 37.0 7 34.87149616611394 37.0 35.0 37.0 32.0 37.0 8 34.900994909275255 37.0 35.0 37.0 32.0 37.0 9 36.288808854903785 39.0 37.0 39.0 32.0 39.0 10-11 36.2067974905473 39.0 37.0 39.0 32.0 39.0 12-13 36.157622356942184 39.0 37.0 39.0 32.0 39.0 14-15 37.03556114150525 40.0 37.0 41.0 31.0 41.0 16-17 36.94055892355463 39.5 37.0 41.0 31.5 41.0 18-19 36.63171458144103 39.0 35.5 41.0 31.5 41.0 20-21 36.633589277793035 39.0 35.0 41.0 31.0 41.0 22-23 36.52385353076615 39.0 35.0 41.0 31.0 41.0 24-25 36.293904860480346 39.0 35.0 41.0 30.5 41.0 26-27 35.97333706512325 38.5 35.0 41.0 30.0 41.0 28-29 35.81198643881625 38.0 35.0 40.5 30.0 41.0 30-31 35.66731268878985 38.0 35.0 41.0 30.0 41.0 32-33 35.24167740436408 38.0 35.0 40.0 29.5 41.0 34-35 34.75870281574112 37.0 35.0 40.0 26.5 41.0 36-37 34.34804925962696 36.0 34.5 40.0 24.5 41.0 38-39 34.066401216704335 36.0 34.5 40.0 24.0 41.0 40-41 34.081662829260054 37.0 35.0 40.0 24.0 41.0 42-43 33.70013307703682 36.0 34.0 40.0 21.5 41.0 44-45 33.32629222027418 36.5 33.0 40.0 16.5 41.0 46-47 32.89634777465622 36.0 33.0 40.0 10.0 41.0 48-49 32.43584313808327 36.0 33.0 40.0 2.0 41.0 50-51 31.17770009083036 35.0 30.5 39.0 2.0 40.0 52-53 31.45529245263091 35.0 31.5 39.0 2.0 40.0 54-55 31.963810439154223 36.0 33.0 40.0 2.0 41.0 56-57 31.70707209395661 35.5 32.0 40.0 2.0 41.0 58-59 31.472101349781376 35.0 32.0 40.0 2.0 41.0 60-61 30.83850151031875 35.0 31.0 39.0 2.0 41.0 62-63 30.549639847067027 35.0 31.0 39.0 2.0 41.0 64-65 30.12084134259944 35.0 29.5 38.5 2.0 40.0 66-67 29.41602416510002 35.0 28.5 37.5 2.0 39.0 68-69 28.83906655964175 35.0 28.0 37.0 2.0 39.0 70-71 28.0030892883547 34.5 26.5 36.0 2.0 38.0 72-73 27.397546524154535 34.0 26.0 35.0 2.0 37.0 74-75 26.939735113326716 34.0 26.0 35.0 2.0 37.0 76-77 26.51603261443569 34.0 25.0 35.0 2.0 36.0 78-79 26.03908345831309 34.0 22.0 35.0 2.0 36.0 80-81 25.446737500264042 33.0 19.0 35.0 2.0 35.5 82-83 24.88452398555164 32.5 12.5 35.0 2.0 35.0 84-85 24.337746350943156 32.0 4.0 35.0 2.0 35.0 86-87 24.051847236011067 32.5 2.0 35.0 2.0 35.0 88-89 23.391367947445133 32.0 2.0 35.0 2.0 35.0 90-91 22.763825225491644 31.0 2.0 35.0 2.0 35.0 92-93 22.112502904459134 31.0 2.0 35.0 2.0 35.0 94-95 21.407416404385206 30.0 2.0 34.0 2.0 35.0 96-97 20.628641135590712 29.0 2.0 34.0 2.0 35.0 98-99 19.95414123064574 29.0 2.0 34.0 2.0 35.0 100-101 17.350958999598657 22.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.5402352066747405 1101 2 -0.3396110037592095 1101 3 0.18040622364208403 1101 4 -0.2193280051714126 1101 5 -0.6308645844477709 1101 6 -0.4209224889223222 1101 7 -0.20575247834901944 1101 8 -0.22710432806137248 1101 9 -0.7036360159177875 1101 10-11 -0.1796550847505145 1101 12-13 -0.5336163860377852 1101 14-15 -0.400221191361652 1101 16-17 -0.020626768256100547 1101 18-19 0.18445565406803155 1101 20-21 0.17953689904150139 1101 22-23 0.20788758737906932 1101 24-25 0.00433507197834615 1101 26-27 -0.1308992853200337 1101 28-29 -0.49919812101191496 1101 30-31 -0.8956491116851808 1101 32-33 -1.1882463065542126 1101 34-35 -1.042703590079448 1101 36-37 -0.42637707086148424 1101 38-39 -1.4558618684329687 1101 40-41 -1.555231475435967 1101 42-43 -0.922474117610939 1101 44-45 -1.2656219131449191 1101 46-47 -1.3415905467689768 1101 48-49 -1.1842365025594113 1101 50-51 -1.0785970223854768 1101 52-53 -1.2953220690921086 1101 54-55 -1.1271057096624908 1101 56-57 -1.2117448230648016 1101 58-59 -1.1510119661593379 1101 60-61 -0.802446009025843 1101 62-63 -0.8618210874128494 1101 64-65 -0.5335198310342619 1101 66-67 -0.05830908376891131 1101 68-69 0.10748456438513188 1101 70-71 -0.9687137735143381 1101 72-73 -1.249459143763449 1101 74-75 -0.6915396813620589 1101 76-77 -0.09595015260503459 1101 78-79 -0.16733519696416366 1101 80-81 -0.39308922494993226 1101 82-83 -0.05550692272813862 1101 84-85 -0.13905984735214005 1101 86-87 0.5540485892873264 1101 88-89 0.5659816104307858 1101 90-91 1.143330021411856 1101 92-93 0.021639664925618973 1101 94-95 -0.06417351627936796 1101 96-97 0.30847599129704406 1101 98-99 0.7456568402315824 1101 100-101 0.8495039437156038 1102 1 0.22893315032322903 1102 2 -0.09687942837043195 1102 3 0.2221912135000963 1102 4 -0.1160149558137391 1102 5 -0.1239004194714326 1102 6 -0.5162571745207032 1102 7 0.48052203145490324 1102 8 0.06025199377770463 1102 9 0.1812862288421826 1102 10-11 0.1743679037552326 1102 12-13 0.5284525794794561 1102 14-15 0.2573176862583608 1102 16-17 0.6320439552056953 1102 18-19 0.6177213741491627 1102 20-21 -0.05312030176985871 1102 22-23 0.32668407148995726 1102 24-25 0.4662011977389966 1102 26-27 0.6658552785744902 1102 28-29 1.0258863955533286 1102 30-31 0.40042931968736184 1102 32-33 0.6438699882395724 1102 34-35 0.3116574646872863 1102 36-37 0.3443463909370337 1102 38-39 0.8486006062120666 1102 40-41 0.9946671046857389 1102 42-43 1.2641384584539708 1102 44-45 1.1107810618381748 1102 46-47 0.7939064106346692 1102 48-49 0.326987297305358 1102 50-51 0.2984820851195984 1102 52-53 -0.045896781735784487 1102 54-55 0.21954743435373558 1102 56-57 0.21503002480537603 1102 58-59 -0.4737300188976761 1102 60-61 0.06313884560566407 1102 62-63 0.4727968977122359 1102 64-65 0.48115224469258067 1102 66-67 0.6439897668057988 1102 68-69 0.9291884183405053 1102 70-71 0.8185073218203449 1102 72-73 0.5932724316253264 1102 74-75 0.9244846594087335 1102 76-77 0.9315008277871257 1102 78-79 1.4764105772075737 1102 80-81 1.2664780502360031 1102 82-83 0.8161631246011396 1102 84-85 1.3468765961907962 1102 86-87 1.7036091031480467 1102 88-89 1.6312960120535074 1102 90-91 2.2943104135687165 1102 92-93 2.124682261274504 1102 94-95 2.6325134343629557 1102 96-97 2.860402968984669 1102 98-99 3.470538517040236 1102 100-101 3.6084626996317652 1103 1 -0.19500665815830232 1103 2 -0.3882610973170806 1103 3 -0.2931302639280844 1103 4 -0.1682721377562828 1103 5 -0.3047485726862078 1103 6 -0.5087332347122171 1103 7 -0.11660519152731297 1103 8 -0.38160847133857345 1103 9 0.036005791085685246 1103 10-11 0.18715860142964402 1103 12-13 0.049382812037592316 1103 14-15 0.24158581211334962 1103 16-17 -0.05564414328951983 1103 18-19 -0.04329093775233872 1103 20-21 0.27677025910566044 1103 22-23 -0.3002653129149664 1103 24-25 0.5552572852902955 1103 26-27 0.036101516604588824 1103 28-29 -0.05947612154653825 1103 30-31 -0.36851732873944343 1103 32-33 -0.018305114359606023 1103 34-35 0.40857948931108723 1103 36-37 0.3992711515389402 1103 38-39 0.15680854054858884 1103 40-41 0.19035793915906396 1103 42-43 0.35579372521183217 1103 44-45 1.0105074640269578 1103 46-47 0.5276957403200342 1103 48-49 0.7341008403970122 1103 50-51 1.1862385830676168 1103 52-53 0.9471264740781677 1103 54-55 0.6750193905780861 1103 56-57 0.9700915843129003 1103 58-59 1.3145052752199717 1103 60-61 1.3171744133211227 1103 62-63 1.1901703587245507 1103 64-65 1.133614624993534 1103 66-67 1.762594417968593 1103 68-69 1.324605655002614 1103 70-71 1.6209013026685 1103 72-73 1.4867744836089116 1103 74-75 1.2844025800653682 1103 76-77 1.4466855063096986 1103 78-79 0.9211438193416335 1103 80-81 0.7555341377873042 1103 82-83 0.840376530893888 1103 84-85 0.2144552555615178 1103 86-87 0.3721453548580307 1103 88-89 -0.06453162132542545 1103 90-91 -0.4651971103710899 1103 92-93 0.19465490149338294 1103 94-95 0.5068636395613169 1103 96-97 0.4446026953868589 1103 98-99 0.6881855758637663 1103 100-101 0.599570770767194 1104 1 -0.053419790853240556 1104 2 0.3266499833942724 1104 3 0.49277944879421653 1104 4 0.4369262206568507 1104 5 0.3231584040579776 1104 6 0.0013898843028243846 1104 7 0.06875732557254821 1104 8 0.26025199377770747 1104 9 -0.006949065275463795 1104 10-11 0.6331914331669921 1104 12-13 0.15198199124416334 1104 14-15 0.8749647450818898 1104 16-17 0.13792630814687357 1104 18-19 0.8118390212079944 1104 20-21 0.7821738158772078 1104 22-23 0.7502134832546616 1104 24-25 0.50737766832723 1104 26-27 1.101149396221551 1104 28-29 1.478827572023917 1104 30-31 1.7298410843932501 1104 32-33 1.3556346941219175 1104 34-35 1.1998927588049284 1104 36-37 1.4090522732899728 1104 38-39 1.7250711944473522 1104 40-41 1.3358435752739766 1104 42-43 1.534726693748091 1104 44-45 1.2578398853675807 1104 46-47 1.5880240576934952 1104 48-49 0.8211049443641869 1104 50-51 1.2514232615901868 1104 52-53 1.5070443947348053 1104 54-55 1.4724886108243211 1104 56-57 1.2797359071583152 1104 58-59 0.7497993928670326 1104 60-61 0.9984329632527285 1104 62-63 1.3316204271240082 1104 64-65 1.5929169505749314 1104 66-67 1.2028132962175668 1104 68-69 1.3880119477522719 1104 70-71 1.494977910055642 1104 72-73 0.6109194904488575 1104 74-75 0.9362493652910864 1104 76-77 1.7726772983753634 1104 78-79 1.1058223419134556 1104 80-81 0.49588981494188644 1104 82-83 1.151457242248199 1104 84-85 1.1645236550143245 1104 86-87 1.180079691383341 1104 88-89 1.5489430708770335 1104 90-91 2.147251590039307 1104 92-93 1.3599763789215622 1104 94-95 1.5736899049511912 1104 96-97 0.8839323807493749 1104 98-99 1.6352443993931765 1104 100-101 0.4731685819847051 1105 1 0.2756041264059732 1105 2 -0.0128458149250541 1105 3 0.10234635247876511 1105 4 0.2799772872373296 1105 5 -0.19332511242554062 1105 6 -0.28678076857370627 1105 7 -0.02626529886183704 1105 8 0.24706518059089433 1105 9 -0.3677764731616975 1105 10-11 0.2194228488101757 1105 12-13 0.5866943377212124 1105 14-15 -0.21100810559683936 1105 16-17 -0.30583581208583865 1105 18-19 -0.08098580102859643 1105 20-21 0.29548991154624105 1105 22-23 0.0793666829960955 1105 24-25 0.43821154033757637 1105 26-27 0.6938449359759105 1105 28-29 0.7066879469431129 1105 30-31 0.6666219505858777 1105 32-33 0.6308770987760894 1105 34-35 0.6029955383783019 1105 36-37 0.4469966818226112 1105 38-39 0.6566807613510406 1105 40-41 0.6042986496114011 1105 42-43 0.05857931171835418 1105 44-45 0.2235153863372048 1105 46-47 0.1580305218176008 1105 48-49 0.6629278273635393 1105 50-51 0.2664200295281276 1105 52-53 0.4310262951872943 1105 54-55 0.6868389663511536 1105 56-57 0.5459285380568311 1105 58-59 0.08923054994524904 1105 60-61 -1.3315605726231645 1105 62-63 -0.4374810596245453 1105 64-65 -0.6927973351393533 1105 66-67 0.06926449208052432 1105 68-69 -0.04844571223479832 1105 70-71 0.40680725717910704 1105 72-73 -0.08752911976445787 1105 74-75 0.17412913258262108 1105 76-77 -0.1911882478431295 1105 78-79 -0.8424646651970811 1105 80-81 -0.7241489698027799 1105 82-83 -0.4311542638927186 1105 84-85 0.22237756580940982 1105 86-87 -0.4780974256173067 1105 88-89 -0.48460573326000755 1105 90-91 -1.3694904914086585 1105 92-93 -1.1378904601217492 1105 94-95 -0.7008414460507453 1105 96-97 -1.4588601208666567 1105 98-99 -0.5151111274329345 1105 100-101 0.39320736672937073 1106 1 1.2145540653559088 1106 2 1.0554081533289121 1106 3 1.0600997102321266 1106 4 1.2009785082385491 1106 5 1.0231584040579804 1106 6 1.2196905378975984 1106 7 1.1053586327620906 1106 8 1.0713631048888175 1106 9 1.5205019151166965 1106 10-11 1.446590125977444 1106 12-13 1.1228970239238976 1106 14-15 1.4154876208988867 1106 16-17 1.585311929061909 1106 18-19 1.959551439508644 1106 20-21 1.4119123779687115 1106 22-23 1.3590370126664268 1106 24-25 1.936135838261869 1106 26-27 1.4736984158293964 1106 28-29 1.6069321471873081 1106 30-31 1.5376842216481492 1106 32-33 0.8634778313768265 1106 34-35 1.19139602677879 1106 36-37 1.4789869138128466 1106 38-39 1.8878162924865691 1106 40-41 1.8629677582805115 1106 42-43 1.6576025107415546 1106 44-45 1.0039183167401333 1106 46-47 1.1720109857980745 1106 48-49 0.49430755874326593 1106 50-51 0.15665201976011645 1106 52-53 0.5318809960419912 1106 54-55 0.051246780758965826 1106 56-57 -0.4996758575475617 1106 58-59 -0.1966058358911411 1106 60-61 0.5435310024684092 1106 62-63 -0.005307677451160231 1106 64-65 -0.473749716091735 1106 66-67 0.2773231001391352 1106 68-69 0.8739596601705735 1106 70-71 1.3577230080948546 1106 72-73 0.6566711244357855 1106 74-75 -0.033685275231789547 1106 76-77 0.4429387362838568 1106 78-79 0.49013606740365034 1106 80-81 0.2475238018699919 1106 82-83 0.10962717688871848 1106 84-85 0.4253079687398156 1106 86-87 0.34772675020686705 1106 88-89 0.006786208131938309 1106 90-91 0.4655522436340753 1106 92-93 0.18677376454247607 1106 94-95 -0.5233689185782211 1106 96-97 -0.6539761159826512 1106 98-99 -0.700703313025123 1106 100-101 0.6326457061677146 1107 1 0.012214884069361887 1107 2 -0.26096611567693273 1107 3 -0.5901926874286936 1107 4 0.5582884497590186 1107 5 0.7442110356369227 1107 6 0.9729069121666072 1107 7 0.8644229602474738 1107 8 1.0286730464092813 1107 9 0.6345370028359909 1107 10-11 0.01647316691312284 1107 12-13 0.6389788952689273 1107 14-15 0.730692299261456 1107 16-17 0.538235905670092 1107 18-19 0.12855728746185946 1107 20-21 0.32273109141900136 1107 22-23 -0.10148930312305282 1107 24-25 0.17239314820339047 1107 26-27 -0.3771787771530555 1107 28-29 0.6952362407545678 1107 30-31 0.8025965023498998 1107 32-33 0.8178637962891031 1107 34-35 0.7258989507554006 1107 36-37 0.9257705395438407 1107 38-39 1.4334303275742926 1107 40-41 1.766184132549519 1107 42-43 1.446491399630439 1107 44-45 1.1083042816524156 1107 46-47 1.4892624477863734 1107 48-49 1.765067792661398 1107 50-51 1.7613303823332167 1107 52-53 2.390945323527376 1107 54-55 1.8375040907004845 1107 56-57 1.7292715108734917 1107 58-59 1.6776631699568156 1107 60-61 1.2891450375561284 1107 62-63 1.3721777026657946 1107 64-65 1.3455485295223006 1107 66-67 0.8703055562794866 1107 68-69 0.8350707712816821 1107 70-71 0.7507054642352067 1107 72-73 0.6660278495819831 1107 74-75 0.1300574148266911 1107 76-77 0.9426463386230353 1107 78-79 0.03399571652645861 1107 80-81 -0.02732999929959945 1107 82-83 0.2458844868302421 1107 84-85 0.4270623547047272 1107 86-87 0.5240425396805506 1107 88-89 0.6901195414652683 1107 90-91 0.6608738810609758 1107 92-93 0.3896977411506626 1107 94-95 -0.5628426027887485 1107 96-97 -0.2510521393744618 1107 98-99 0.2829224179690293 1107 100-101 0.30165155412093014 1108 1 -0.0994555964798387 1108 2 0.007340520478674506 1108 3 0.2523702416330913 1108 4 0.9007369623448298 1108 5 0.6709844910145009 1108 6 0.5112364316174052 1108 7 0.47540694194083954 1108 8 0.8645998198646581 1108 9 0.6980381470007515 1108 10-11 0.9428461646247897 1108 12-13 0.9099743186098905 1108 14-15 0.719593901092118 1108 16-17 0.8930413976609444 1108 18-19 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-2.449846891393406 2316 76-77 -2.1989804556353363 2316 78-79 -2.0447124174448312 2316 80-81 -2.5519711476249576 2316 82-83 -2.4560293887678455 2316 84-85 -2.223176879745033 2316 86-87 -2.711364158884038 2316 88-89 -2.596244094898367 2316 90-91 -2.9915184634366305 2316 92-93 -2.5440343162656056 2316 94-95 -2.273368918578221 2316 96-97 -3.507511469518004 2316 98-99 -3.21635987868169 2316 100-101 -2.306243182721179 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1452.0 3 249.0 4 237.0 5 224.0 6 207.0 7 246.0 8 256.0 9 289.0 10 378.0 11 491.0 12 731.0 13 930.0 14 949.0 15 1137.0 16 1399.0 17 1641.0 18 1339.0 19 1055.0 20 1040.0 21 1221.0 22 1183.0 23 1307.0 24 1493.0 25 1635.0 26 1830.0 27 2024.0 28 2251.0 29 2662.0 30 3016.0 31 3444.0 32 4228.0 33 5081.0 34 7299.0 35 13735.0 36 10124.0 37 11931.0 38 5936.0 39 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.05511160099201 20.939228861521503 18.43059117790449 10.575068359582 2 14.382381239360972 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31.815334968100807 22.78006008301222 46-47 21.781290137675544 33.357393824238116 32.73482862207692 12.126487416009427 48-49 18.443081986357726 30.438198370358577 33.86787695410993 17.25084268917377 50-51 10.328683355627746 32.811007070514044 39.98874662929698 16.871562944561227 52-53 16.76477925993556 35.732978040119114 28.680549288971225 18.821693410974103 54-55 13.277957645560216 28.92521628363675 33.60968101480813 24.187145055994904 56-57 19.82349163893798 26.976166595023166 31.69933079662267 21.501010969416186 58-59 25.124969487280175 26.99181728452713 30.85233966229052 17.03087356590217 60-61 17.344968436687708 30.115113256591165 37.55981115060209 14.980107156119038 62-63 13.82070239314697 30.510633555009477 36.469351902469576 19.19931214937398 64-65 13.53275294617263 36.5638602824079 31.894574795625864 18.00881197579361 66-67 17.67500875972861 28.797740520911862 28.655461292617407 24.871789426742126 68-69 14.197953668158672 29.28686344159671 33.32005925548352 23.195123634761096 70-71 21.903356146563947 29.510303120403947 29.175803462867883 19.410537270164223 72-73 18.75836147034465 24.563079994054064 38.50311099785522 18.175447537746066 74-75 14.396215797241481 35.7550886059821 31.228697932704048 18.619997664072372 76-77 16.952031165412336 27.684248516564587 31.151082191344592 24.212638126678485 78-79 18.22533566841798 28.359072334553947 34.02483680942525 19.390755187602824 80-81 21.961523152447924 26.302772986599443 31.540931405068328 20.194772455884305 82-83 18.177716918243 25.401248328131963 36.93395325138526 19.48708150223977 84-85 17.84976939448139 28.919365025448872 33.70927251787259 19.521593062197148 86-87 18.094727568015117 29.757873618733218 31.811330368231662 20.336068445020008 88-89 17.41558124475866 29.429529845121706 28.354723310297974 24.80016559982166 90-91 20.348763138337404 24.223909119864103 30.574901794245672 24.85242594755282 92-93 23.301037878586786 28.048204284235396 31.586547394685848 17.06421044249197 94-95 21.128218741704273 25.962835147332093 32.238916910007966 20.670029200955668 96-97 15.943790877431411 27.063513972649282 36.15794614796569 20.83474900195362 98-99 20.573433701041548 29.172815781371103 28.8898680284965 21.36388248909085 100-101 19.550240961297582 24.189011315627454 31.596182833365177 24.664564889709784 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8170.0 1 4570.0 2 912.0 3 729.0 4 505.5 5 510.5 6 889.5 7 1602.5 8 1846.5 9 1407.5 10 832.0 11 391.5 12 269.5 13 249.5 14 239.0 15 246.5 16 281.5 17 256.5 18 202.5 19 200.0 20 214.5 21 224.5 22 214.5 23 234.5 24 267.0 25 294.0 26 332.0 27 378.5 28 435.0 29 512.5 30 593.0 31 653.5 32 688.0 33 697.0 34 674.5 35 638.5 36 591.5 37 534.0 38 478.0 39 391.0 40 318.5 41 263.5 42 241.0 43 254.0 44 284.0 45 529.5 46 2247.0 47 7626.0 48 16170.0 49 16447.5 50 8892.5 51 4246.5 52 2207.5 53 1433.0 54 1028.0 55 677.0 56 431.5 57 293.5 58 235.0 59 196.0 60 147.5 61 133.5 62 128.5 63 124.5 64 104.5 65 98.5 66 102.5 67 83.5 68 77.5 69 64.0 70 56.0 71 51.5 72 43.0 73 33.0 74 27.0 75 26.0 76 19.5 77 18.5 78 14.0 79 9.5 80 8.0 81 6.0 82 3.5 83 3.0 84 2.5 85 0.5 86 0.0 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34642276251029763 2 0.1066728628461587 3 0.11512219851714159 4 0.1225153672292516 5 0.12462770114699732 6 0.07076318624448152 7 0.06970701928560867 8 0.07287552016222724 9 0.07498785407997297 10-11 0.07287552016222724 12-13 0.0702351027650451 14-15 0.06759468536786295 16-17 0.0702351027650451 18-19 0.08079677235377368 20-21 0.08026868887433726 22-23 0.09822352717517586 24-25 0.11089753068165016 26-27 0.10033586109292156 28-29 0.09663927673686656 30-31 0.08924610802475655 32-33 0.0829091062715194 34-35 0.32952409116833187 36-37 0.5106567246150271 38-39 0.4948142202319343 40-41 0.47949979932827785 42-43 0.5043197228617899 44-45 0.5059039733000993 46-47 0.5016793054646079 48-49 0.5169937263682642 50-51 0.515409475929955 52-53 0.5148813924505186 54-55 0.5037916393823535 56-57 0.4916457193553157 58-59 0.4847806341226421 60-61 0.45098329143871063 62-63 0.5022073889440443 64-65 0.5196341437654464 66-67 0.5291396463953021 68-69 0.5423417333812128 70-71 0.5402293994634673 72-73 0.5280834794364293 74-75 0.5291396463953021 76-77 0.5016793054646079 78-79 0.493229969793625 80-81 0.4958703871908071 82-83 0.5027354724234807 84-85 0.5000950550262986 86-87 0.5016793054646079 88-89 0.5059039733000993 90-91 0.5196341437654464 92-93 0.5275553959569929 94-95 0.5354766481485393 96-97 0.5259711455186835 98-99 0.5228026446420649 100-101 0.5027354724234807 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 94682.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.807545256754185 #Duplication Level Percentage of deduplicated Percentage of total 1 85.20887264605858 17.729874738598678 2 6.629105121567433 2.7587080965759068 3 2.568397543271915 1.6032614435689996 4 1.5481447642251662 1.2885236898248875 5 0.8882797827521445 0.9241460890137512 6 0.43145018019389875 0.5386451490251579 7 0.3299324907365109 0.4805559662871507 8 0.27409776153494747 0.45626412623307494 9 0.2233389168062535 0.41824211571365205 >10 1.3958682300390843 5.786738767664392 >50 0.1573524186589513 2.2042204431676558 >100 0.20303537891477588 9.069305675841237 >500 0.030455306837216384 4.441182062060371 >1k 0.09644180498451856 33.321011385479814 >5k 0.015227653418608192 18.97932025094527 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6810 7.192496989924167 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5644 5.961006315878414 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5516 5.825816945142688 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4317 4.559472761454131 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2806 2.9636044865972413 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2021 2.1345134238820473 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1961 2.071143406349676 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1817 1.919055364271984 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1738 1.835618174521028 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1721 1.8176633362201897 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1699 1.7944276631249867 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1574 1.6624067932658793 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1508 1.5926997739802706 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1345 1.4205445596839947 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1260 1.3307703681798018 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1227 1.2959168585369976 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1213 1.2811305211127775 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1161 1.226209839251389 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1114 1.1765699921843646 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1055 1.1142561416108658 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1007 1.0635601275849687 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1005 1.061447793667223 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 820 0.8660569062757442 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 797 0.8417650662216682 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 705 0.7445977060053653 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 684 0.7224181998690353 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 675 0.7129126972391796 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 524 0.5534314864493779 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 487 0.5143533089710822 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 474 0.5006231385057349 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 464 0.4900614689170064 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 400 0.42246678354914347 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 397 0.4192982826725249 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 357 0.37705160431761053 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 294 0.3105130859086204 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 286 0.3020637502376376 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 286 0.3020637502376376 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 281 0.2967829154432733 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 262 0.27671574322468895 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 261 0.2756595762658161 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 251 0.26509790667708755 No Hit GTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 241 0.25453623708835893 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 217 0.22918823007541034 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 199 0.21017722481569884 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 198 0.20912105785682603 No Hit TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 178 0.18799771867936882 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 177 0.18694155172049598 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 176 0.18588538476162314 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 176 0.18588538476162314 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 171 0.18060454996725883 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 163 0.17215521429627595 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 162 0.1710990473374031 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 158 0.16687437950191167 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 149 0.15736887687205595 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 145 0.1531442090365645 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 138 0.1457510403244545 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 137 0.14469487336558162 No Hit TGATACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 133 0.1404702055300902 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 130 0.13730170465347163 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 128 0.1351893707357259 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 128 0.1351893707357259 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 117 0.12357153418812446 No Hit CCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 117 0.12357153418812446 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 116 0.1225153672292516 No Hit ACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 111 0.11723453243488731 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 111 0.11723453243488731 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 106 0.11195369764052301 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 105 0.11089753068165016 No Hit TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 100 0.10561669588728587 No Hit ACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 98 0.10350436196954013 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 95 0.10033586109292156 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.280834794364293E-4 0.0 12-13 0.0 0.0 0.0 0.0010561669588728586 0.0 14-15 0.0 0.0 0.0 0.0010561669588728586 0.0 16-17 0.0 0.0 0.0 0.0015842504383092879 0.0 18-19 0.0 0.0 0.0 0.0031685008766185758 0.0 20-21 0.0 0.0 0.0 0.00739316871211001 0.0 22-23 0.0 0.0 0.0 0.01584250438309288 0.0 24-25 0.0 0.0 0.0 0.026932257451257893 0.0 26-27 0.0 0.0 0.0 0.06072960013518937 0.0 28-29 0.0 0.0 0.0 0.18218880040556812 0.0 30-31 0.0 0.0 0.0 0.4251072009463256 0.0 32-33 0.0 0.0 0.0 0.7445977060053652 0.0 34-35 0.0 0.0 0.0 1.1517500686508524 0.0 36-37 0.0 0.0 0.0 1.6571259584715152 0.0 38-39 0.0 0.0 0.0 2.3404659808622545 0.0 40-41 0.0 0.0 0.0 2.990536744048499 0.0 42-43 0.0 0.0 0.0 3.6099786654274304 0.0 44-45 0.0 0.0 0.0 4.291734437379861 0.0 46-47 0.0 0.0 0.0 4.9808833780444015 0.0 48-49 0.0 0.0 0.0 5.456158509537188 0.0 50-51 0.0 0.0 0.0 5.974736486343762 0.0 52-53 0.0 0.0 0.0 6.46004520394584 0.0 54-55 0.0 0.0 0.0 7.005027354724234 0.0 56-57 0.0 0.0 0.0 7.77919773557804 0.0 58-59 0.0 0.0 0.0 8.310449715891089 0.0 60-61 0.0 0.0 0.0 8.900847045901017 0.0 62-63 0.0 0.0 0.0 9.513423882047274 0.0 64-65 0.0 0.0 0.0 10.062102617181726 0.0 66-67 0.0 0.0 0.0 10.752835808284573 0.0 68-69 0.0 0.0 0.0 11.662195559874105 0.0 70-71 0.0 0.0 0.0 12.481253036480005 0.0 72-73 0.0 0.0 0.0 13.117065545721466 0.0 74-75 0.0 0.0 0.0 14.016919794681144 0.0 76-77 0.0 0.0 0.0 14.63108088126571 0.0 78-79 0.0 0.0 0.0 15.78177478295769 0.0 80-81 0.0 0.0 0.0 16.856424663610824 0.0 82-83 0.0 0.0 0.0 17.650662216683216 0.0 84-85 0.0 0.0 0.0 18.60068439618935 0.0 86-87 0.0 0.0 0.0 19.50001056166959 0.0 88-89 0.0 0.0 0.0 20.44633615681967 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 295 0.0 43.35104 9 CATAAGC 30 8.378105E-5 39.49172 16-17 CATTAAA 25 0.0016056014 38.03266 94-95 TACATAA 25 0.0016351052 37.892025 14-15 GGCTTGA 160 1.8189894E-12 35.523773 8 GGGCTTG 165 1.8189894E-12 34.447296 7 CAGAAAA 250 0.0 34.10282 10-11 GTAAGCG 45 2.3882953E-5 31.693884 94-95 TGGGCTT 180 5.456968E-12 31.576687 6 GCTTTGC 185 9.094947E-12 30.77203 1 GCTTGAT 185 9.094947E-12 30.723263 9 CTTTGCG 190 1.2732926E-11 29.930567 2 TTTGCGT 190 1.2732926E-11 29.930567 3 GAGTAAA 40 4.5549026E-4 29.603144 12-13 TGCTTTG 125 7.460707E-6 26.524416 7 ATGGGCT 200 7.166818E-10 26.064535 5 CATGGGC 200 7.166818E-10 26.064535 4 TTGCGTT 250 1.4551915E-11 24.642834 4 GAGTACT 760 0.0 24.305738 12-13 CGTATCA 50 0.0016403194 23.770412 92-93 >>END_MODULE