##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939057_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 94682 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.836294121374706 31.0 31.0 34.0 30.0 34.0 2 32.00951606429945 33.0 31.0 34.0 30.0 34.0 3 32.04365138041022 34.0 31.0 34.0 30.0 34.0 4 35.61737183413954 37.0 35.0 37.0 33.0 37.0 5 35.56229272723432 37.0 35.0 37.0 33.0 37.0 6 35.724076381994465 37.0 35.0 37.0 33.0 37.0 7 35.695602120783256 37.0 35.0 37.0 33.0 37.0 8 35.740594833231235 37.0 35.0 37.0 33.0 37.0 9 37.23100483724467 39.0 37.0 39.0 33.0 39.0 10-11 37.1427092794829 39.0 37.0 39.0 33.0 39.0 12-13 37.12866225892989 39.0 37.0 39.0 33.0 39.0 14-15 38.024085887497094 40.0 37.0 41.0 32.5 41.0 16-17 38.119003612091 39.5 37.0 41.0 33.0 41.0 18-19 37.89305253374454 40.0 36.5 41.0 33.0 41.0 20-21 37.78633214338522 40.0 36.0 41.0 33.0 41.0 22-23 37.64738809911071 39.5 35.0 41.0 33.0 41.0 24-25 37.414455757166095 39.5 35.0 41.0 32.5 41.0 26-27 37.041882300754104 38.5 35.0 41.0 32.5 41.0 28-29 36.82833590333961 38.0 35.0 40.5 32.0 41.0 30-31 36.646210472951566 38.0 35.0 41.0 31.0 41.0 32-33 36.17058680636235 38.0 35.0 40.0 30.5 41.0 34-35 35.823778542912066 38.0 35.0 40.0 30.0 41.0 36-37 35.55746076339747 37.5 35.0 40.0 30.0 41.0 38-39 35.26845123677151 37.0 35.0 40.0 29.5 41.0 40-41 35.30890243129634 38.0 35.0 40.0 29.5 41.0 42-43 34.91739190131176 37.5 35.0 40.0 26.0 41.0 44-45 34.5613791428149 37.0 35.0 40.0 24.5 41.0 46-47 34.11321053632159 37.0 33.5 40.0 23.0 41.0 48-49 33.61185864261422 37.0 33.0 40.0 21.5 41.0 50-51 33.20421516233286 36.0 33.0 40.0 20.0 41.0 52-53 33.12263682642953 37.0 33.0 40.0 19.5 41.0 54-55 32.89035402716462 36.5 33.0 40.0 14.0 41.0 56-57 32.60777127648339 35.5 32.5 40.0 10.5 41.0 58-59 32.29922794195306 36.0 32.5 39.0 5.0 41.0 60-61 31.578816459305887 35.0 31.0 39.0 2.0 40.0 62-63 31.16858008914049 35.0 31.0 39.0 2.0 40.0 64-65 30.75480027882808 35.0 30.5 38.5 2.0 39.5 66-67 29.976722080226445 35.0 29.5 37.0 2.0 39.0 68-69 29.327358948902642 35.0 29.0 36.5 2.0 39.0 70-71 28.501483914577214 34.0 27.5 35.5 2.0 38.0 72-73 27.858172619927757 33.5 26.5 35.0 2.0 37.0 74-75 27.445052913964638 33.0 26.0 35.0 2.0 37.0 76-77 25.68962421579603 31.0 24.5 33.5 2.0 35.0 78-79 26.61536511691768 33.0 26.0 35.0 2.0 35.0 80-81 26.517194398090453 33.0 25.0 35.0 2.0 35.0 82-83 26.08424515747449 33.0 24.5 35.0 2.0 35.0 84-85 25.58280348957563 32.0 22.0 35.0 2.0 35.0 86-87 25.16187342895165 32.0 19.5 35.0 2.0 35.0 88-89 24.39886673285313 31.5 14.5 34.0 2.0 35.0 90-91 23.73348682959802 31.0 4.5 34.0 2.0 35.0 92-93 23.36747745083543 31.0 2.0 34.0 2.0 35.0 94-95 22.75226019729199 31.0 2.0 34.0 2.0 35.0 96-97 21.797443019792567 30.0 2.0 34.0 2.0 35.0 98-99 20.74084303246657 29.0 2.0 34.0 2.0 35.0 100-101 15.625958471515178 13.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.34220776018145926 1101 2 0.1086167585156872 1101 3 0.05581418245503045 1101 4 -0.05454976731662953 1101 5 0.11596908371221559 1101 6 0.18699216460667145 1101 7 0.39408564316684647 1101 8 0.48727995819498915 1101 9 0.4525601085982416 1101 10-11 0.25749945805362273 1101 12-13 0.14520570106090958 1101 14-15 0.48253839732536363 1101 16-17 0.4923976754935282 1101 18-19 0.08636293990905841 1101 20-21 0.25807872555450473 1101 22-23 0.24202976832637546 1101 24-25 0.2979251743534732 1101 26-27 0.043483909441398794 1101 28-29 -0.12796355993178565 1101 30-31 0.10769526393081463 1101 32-33 0.5944203825268275 1101 34-35 0.8238317647457336 1101 36-37 0.6046143881598454 1101 38-39 0.4512966360747228 1101 40-41 0.5305533588577376 1101 42-43 0.23776538929002555 1101 44-45 0.24860010939531207 1101 46-47 0.3904972729508245 1101 48-49 0.5320608357200527 1101 50-51 0.6421864920597571 1101 52-53 0.5949585806637074 1101 54-55 0.6537686337509214 1101 56-57 0.7727734974751925 1101 58-59 0.6235607158109246 1101 60-61 -0.38669128400732333 1101 62-63 0.5543631277620342 1101 64-65 0.9329956818316916 1101 66-67 -0.20988843181154238 1101 68-69 -0.038978552243573716 1101 70-71 0.025836548520143054 1101 72-73 -0.260322045000418 1101 74-75 -0.4283436482947458 1101 76-77 -0.131782473079177 1101 78-79 0.2433898230609124 1101 80-81 0.6129229910167879 1101 82-83 -0.057446117413082476 1101 84-85 0.19745042834188808 1101 86-87 0.5439964900649983 1101 88-89 1.3579400434089663 1101 90-91 1.5968202757743235 1101 92-93 0.41582845979944594 1101 94-95 0.389701640416277 1101 96-97 1.113297847159 1101 98-99 1.1615459186039097 1101 100-101 1.4235317106429939 1102 1 -0.03591972772709795 1102 2 -0.02904382295827901 1102 3 -0.411799069742397 1102 4 -0.14690946981831843 1102 5 -0.24427432399569682 1102 6 0.13831062302452324 1102 7 0.420319585019449 1102 8 -0.024417134435168464 1102 9 0.16398674821960668 1102 10-11 0.10827701045388949 1102 12-13 0.25223747928944107 1102 14-15 0.3480556387046789 1102 16-17 -0.180286570618712 1102 18-19 -0.0016019011997983057 1102 20-21 0.3178488404970352 1102 22-23 0.20416634709582127 1102 24-25 0.5156398464292025 1102 26-27 0.5226590277645897 1102 28-29 -0.010789793873641429 1102 30-31 0.5557682862431932 1102 32-33 0.26507623107314515 1102 34-35 -0.0779937930635981 1102 36-37 -0.20681225146151405 1102 38-39 0.07076925270757783 1102 40-41 0.18349453532832882 1102 42-43 0.36271467935088 1102 44-45 0.7544824623364832 1102 46-47 0.5878603561151223 1102 48-49 0.6791872725016646 1102 50-51 0.5933697239732112 1102 52-53 0.6723081411775595 1102 54-55 0.5981905404446266 1102 56-57 0.10860852113983555 1102 58-59 0.2064545630049821 1102 60-61 0.1486704468919342 1102 62-63 -0.425623349587525 1102 64-65 -0.07748437225890825 1102 66-67 -0.1139452269433896 1102 68-69 0.5505413936658243 1102 70-71 0.36944709618748206 1102 72-73 -0.12110635872590692 1102 74-75 0.24920875197571135 1102 76-77 0.15888689811757573 1102 78-79 0.6473790049405608 1102 80-81 -0.09275652216779662 1102 82-83 0.07000486297907926 1102 84-85 -0.05177201925784303 1102 86-87 0.4884860100109094 1102 88-89 0.6107459933751578 1102 90-91 0.7441495523125283 1102 92-93 0.8504464449245326 1102 94-95 1.4558274010653633 1102 96-97 1.2894979823855017 1102 98-99 1.813134830030549 1102 100-101 2.0147419878573274 1103 1 0.2684578372523809 1103 2 0.3429124013919207 1103 3 0.05728437759002247 1103 4 0.09166782156334818 1103 5 0.08910460897283912 1103 6 1.4372835968146092E-4 1103 7 0.12798032373353863 1103 8 0.327429650790549 1103 9 0.08902094794601112 1103 10-11 0.4599185973211988 1103 12-13 -0.06643557132615285 1103 14-15 0.6515440107977 1103 16-17 0.4070595306124787 1103 18-19 0.5283570591285169 1103 20-21 0.6538953521249411 1103 22-23 0.8454112171368564 1103 24-25 0.783423704158345 1103 26-27 0.7366809155894884 1103 28-29 0.41540719655044 1103 30-31 0.6866848389107716 1103 32-33 0.6148026332619239 1103 34-35 1.0409528553632157 1103 36-37 0.8635023860213877 1103 38-39 0.6353383361549092 1103 40-41 1.158460335601923 1103 42-43 0.8925368407735874 1103 44-45 0.7311582489301927 1103 46-47 0.3979150756773606 1103 48-49 0.8722105283156196 1103 50-51 0.6820154148076796 1103 52-53 1.0866720262664842 1103 54-55 1.0797910876402526 1103 56-57 0.5114128987048119 1103 58-59 -0.38198592947108523 1103 60-61 -0.2790997309466441 1103 62-63 -0.5287697244165237 1103 64-65 0.2563049574264511 1103 66-67 0.5439207101291146 1103 68-69 0.9645632814907223 1103 70-71 0.7595975749836548 1103 72-73 0.509057799960825 1103 74-75 0.5896328285830954 1103 76-77 -0.28755633033659933 1103 78-79 0.3180356396874835 1103 80-81 0.49965113857091836 1103 82-83 0.5898407042923459 1103 84-85 0.708009102493186 1103 86-87 0.1938895667824525 1103 88-89 0.8811290303108663 1103 90-91 0.42834927871471606 1103 92-93 0.12479665012289232 1103 94-95 0.24214751050448768 1103 96-97 -0.4217195278744157 1103 98-99 -1.5019130495864133 1103 100-101 -0.17383530352434207 1104 1 -0.03591972772709795 1104 2 0.006250294688776137 1104 3 0.24702445966936892 1104 4 -0.11161535217126328 1104 5 -0.10309785340746203 1104 6 0.2677223877304087 1104 7 0.22031958501944615 1104 8 0.15205345380012147 1104 9 0.4581043952784327 1104 10-11 0.3494534810421257 1104 12-13 -0.024233108945857396 1104 14-15 -0.1342973024717935 1104 16-17 0.20794872349893723 1104 18-19 0.3278098635060829 1104 20-21 0.44726060520292066 1104 22-23 -0.08995129996300477 1104 24-25 0.4332869052527357 1104 26-27 -0.18910567811776247 1104 28-29 0.2539160884793006 1104 30-31 0.5969447568314266 1104 32-33 -0.12315906304450408 1104 34-35 -0.44858202835771266 1104 36-37 -0.6126946044026909 1104 38-39 -0.8233483943512496 1104 40-41 0.130553358857739 1104 42-43 0.21565585582146696 1104 44-45 0.17801187410119468 1104 46-47 0.07609565023277298 1104 48-49 0.34977550779578337 1104 50-51 0.4580756063261546 1104 52-53 0.6076022588246204 1104 54-55 0.3099552463269859 1104 56-57 -0.1384503023895789 1104 58-59 0.5535133865343909 1104 60-61 0.21337632924487693 1104 62-63 0.38025900335365037 1104 64-65 0.7813391571528534 1104 66-67 0.4742900671742589 1104 68-69 0.44465904072464824 1104 70-71 0.7223882726580726 1104 72-73 0.4318348177446829 1104 74-75 0.8786205166815897 1104 76-77 0.3353574863528692 1104 78-79 1.0650260637640905 1104 80-81 1.013125830773376 1104 82-83 0.9876519218026054 1104 84-85 0.8188162160362786 1104 86-87 0.9237801276579667 1104 88-89 1.128393052198689 1104 90-91 0.3794436699595849 1104 92-93 1.1386817390421804 1104 94-95 1.2499450481241894 1104 96-97 0.6189097470913829 1104 98-99 1.3601936535599606 1104 100-101 2.4559184584455593 1105 1 -0.3869216669643336 1105 2 0.03611454808244474 1105 3 0.2370697085381437 1105 4 0.05205627032388804 1105 5 0.1735666585511666 1105 6 -0.6671192412288676 1105 7 -0.7208568855687858 1105 8 -0.24911008853988648 1105 9 0.013631221393495707 1105 10-11 -0.1285684969798524 1105 12-13 0.12108040107353446 1105 14-15 -0.35963666898762625 1105 16-17 -0.5641262603407498 1105 18-19 -0.03502142285461218 1105 20-21 -0.09533797268978361 1105 22-23 -0.18174186234180922 1105 24-25 -0.2736297075462346 1105 26-27 -0.08794213577775167 1105 28-29 -0.028178287732721685 1105 30-31 -0.4161774151142694 1105 32-33 -0.8287828510212307 1105 34-35 -0.5848457646214484 1105 36-37 -0.7037094718881463 1105 38-39 -0.33233352686578854 1105 40-41 -0.2606554323510508 1105 42-43 -0.28137064708739246 1105 44-45 -0.4217295609343594 1105 46-47 -0.4122689263671049 1105 48-49 -1.0449885516741588 1105 50-51 -0.013870095031307983 1105 52-53 -0.24184829062592428 1105 54-55 -0.08306349963293513 1105 56-57 -0.16779742585434576 1105 58-59 -0.06975746026586194 1105 60-61 -0.1780263856872537 1105 62-63 0.28071149204143353 1105 64-65 -0.997975645691362 1105 66-67 -0.020991122224575776 1105 68-69 -0.030389440206189278 1105 70-71 -0.039150188880388015 1105 72-73 -0.2346810193593889 1105 74-75 -0.2325624180307564 1105 76-77 -0.4714944852049854 1105 78-79 -0.8794471101208501 1105 80-81 -0.3929504458911346 1105 82-83 -0.6746622346291993 1105 84-85 -0.8699362080102624 1105 86-87 -0.44570920653724855 1105 88-89 -0.3286205224619465 1105 90-91 -0.9036849661102266 1105 92-93 -0.8576337102144471 1105 94-95 -0.3876761542028966 1105 96-97 -1.0949881197476579 1105 98-99 -0.4513124873579457 1105 100-101 -0.4446633127244439 1106 1 0.21832863828597127 1106 2 0.303635915603806 1106 3 0.09604406751250139 1106 4 0.2151820334496506 1106 5 0.27075835574286344 1106 6 0.21151323740360795 1106 7 0.5346986699867671 1106 8 0.43440639497659816 1106 9 0.6724834802457522 1106 10-11 0.20500903659768 1106 12-13 0.5296884596816014 1106 14-15 0.8147223053713475 1106 16-17 0.2370336908191959 1106 18-19 0.7304242425910488 1106 20-21 0.8011821738303624 1106 22-23 0.3678918372918929 1106 24-25 0.5617182778017522 1106 26-27 0.40076360292798796 1106 28-29 0.3977069381525027 1106 30-31 0.2923695934327313 1106 32-33 -0.16956429180266497 1106 34-35 0.512529082753403 1106 36-37 0.7069132387345647 1106 38-39 1.0737104291781705 1106 40-41 0.7749978033021776 1106 42-43 -0.17813499385173515 1106 44-45 -0.7680665572713536 1106 46-47 -1.1474337615319428 1106 48-49 -1.4208127274983369 1106 50-51 -0.8664341975954173 1106 52-53 -0.9146199633975982 1106 54-55 -1.9508290673985087 1106 56-57 -1.3391038971608182 1106 58-59 -0.6549833454917504 1106 60-61 -0.7895648472257122 1106 62-63 -0.5184338071038646 1106 64-65 -0.6882686859844007 1106 66-67 -0.8708079720414297 1106 68-69 -0.5880206978374432 1106 70-71 -0.26094506067525813 1106 72-73 -0.1769887116670823 1106 74-75 0.17012378465544842 1106 76-77 0.24254702883652968 1106 78-79 0.5405162598425228 1106 80-81 0.6876356346949457 1106 82-83 0.4147761048091425 1106 84-85 0.883848895774836 1106 86-87 0.8309696701416271 1106 88-89 0.41924272540130403 1106 90-91 0.10754824512298455 1106 92-93 0.23900853642780007 1106 94-95 -0.4595320760588173 1106 96-97 -0.03631901107855029 1106 98-99 0.04679496074950151 1106 100-101 -0.2140161820773141 1107 1 -0.36471229738654287 1107 2 0.10655989221199746 1107 3 -0.017991020206792996 1107 4 0.04442179953152703 1107 5 -0.2157913518594725 1107 6 -0.42825284446773537 1107 7 -0.01559372767405165 1107 8 -0.11296202607603334 1107 9 -0.44096681215190614 1107 10-11 -0.034757045273664744 1107 12-13 -0.11153961049384264 1107 14-15 0.042792480809943356 1107 16-17 -0.06852186473635413 1107 18-19 0.4696055291407575 1107 20-21 0.18626989312861753 1107 22-23 0.15122517062522434 1107 24-25 -0.007579967812283428 1107 26-27 -0.3188270403468607 1107 28-29 -0.22100651213989408 1107 30-31 0.25143392091811023 1107 32-33 -0.09003212805998118 1107 34-35 -0.30121360730507973 1107 36-37 -0.17027974372157928 1107 38-39 0.30265779759921685 1107 40-41 0.38318493780510465 1107 42-43 0.7379468774932931 1107 44-45 0.1275474778163641 1107 46-47 -0.22111797205825212 1107 48-49 0.27392411460692756 1107 50-51 -0.056785074788393786 1107 52-53 0.6865496272456753 1107 54-55 0.2526797045313174 1107 56-57 0.39189025488596485 1107 58-59 0.026887999537485996 1107 60-61 -0.2974595840678198 1107 62-63 0.1616831519604638 1107 64-65 0.949450612261213 1107 66-67 0.7572622033971719 1107 68-69 0.34063427292278803 1107 70-71 -0.28287488523666227 1107 72-73 0.2572218146487053 1107 74-75 0.4256793402110013 1107 76-77 0.2489797773745437 1107 78-79 0.17648117212322134 1107 80-81 -0.1246450670594399 1107 82-83 0.4916766896044642 1107 84-85 0.39993076711986575 1107 86-87 0.6479287344691116 1107 88-89 0.7373713803720605 1107 90-91 0.29058918079549656 1107 92-93 0.0445640919833572 1107 94-95 -0.7364326608541418 1107 96-97 -0.054155268388491606 1107 98-99 -0.4967722907124852 1107 100-101 -0.3186945446504126 1108 1 -0.2616230525352812 1108 2 -0.04080852884063546 1108 3 -0.059753033936772226 1108 4 -0.1836102370817443 1108 5 -0.36716434957114075 1108 6 -0.08099883989108037 1108 7 0.23566485356165146 1108 8 -0.03733273545818605 1108 9 0.043014881211938416 1108 10-11 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274.0 11 345.0 12 498.0 13 630.0 14 829.0 15 1045.0 16 1365.0 17 1555.0 18 1472.0 19 1158.0 20 1156.0 21 1251.0 22 1448.0 23 1454.0 24 1657.0 25 1687.0 26 1870.0 27 2048.0 28 2313.0 29 2688.0 30 3085.0 31 3611.0 32 4309.0 33 5635.0 34 9093.0 35 12571.0 36 11955.0 37 13270.0 38 3838.0 39 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.382340461642904 21.6936099796334 18.818949422946368 11.105100135777326 2 14.223400435140787 31.253036480006756 31.12735261190089 23.396210472951566 3 29.563169345810188 27.441329925434616 34.560951395196554 8.434549333558648 4 10.171943980904501 22.30202150355928 41.12502904459137 26.40100547094485 5 6.818613886483176 44.403371284932724 35.506220823387764 13.271794005196341 6 20.09991339430937 39.8280560190955 15.6471134957014 24.42491709089373 7 15.648169662660274 41.141927715933335 24.131302676327074 19.07859994507932 8 21.05257599121269 24.667835491434488 21.64614182209924 32.63344669525359 9 14.869774613970977 28.642191757673054 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21.209800514426657 29.36668480011831 32.28898736116026 17.134527324294776 36-37 16.956404608338747 28.656323788092486 39.078966134732106 15.308305468836664 38-39 15.905333819234283 32.219458220440586 36.20543047127111 15.669777489054024 40-41 15.214073334706443 34.53586197062598 33.257812211184515 16.992252483483057 42-43 14.29356063606741 34.34045766856262 28.946242692143166 22.419739003226812 44-45 17.61531068135489 27.65343951668269 31.885634618025115 22.845615183937305 46-47 21.887294638283493 33.13881950348805 32.46708667571464 12.506799182513822 48-49 18.764754033429274 30.267486995326237 33.823770167146364 17.14398880409812 50-51 10.008871896156569 32.77003833926553 40.149554820925 17.071534943652896 52-53 16.280285587545677 36.1895608457785 28.997063855854332 18.53308971082149 54-55 13.062672947339514 29.35880103926829 32.90963435499884 24.668891658393356 56-57 19.519549650408738 27.541137703048097 31.09091485181977 21.84839779472339 58-59 24.61739947824801 27.918484173170967 30.401030829839144 17.06308551874188 60-61 16.92798411499728 32.06204023003681 36.364404497230154 14.645571157735754 62-63 12.98267849598648 32.37114490916772 35.362272919307145 19.283903675538657 64-65 12.487655983481462 38.792689173703415 30.56140851169447 18.158246331120655 66-67 16.78082372636104 30.914295069154356 27.269894750183514 25.03498645430109 68-69 13.630194498281043 31.17359090404998 31.70010403519241 23.496110562476566 70-71 20.95804904839357 31.299507826197164 27.71540525126212 20.027037874147144 72-73 17.797997507445977 26.458566570203416 36.84808094463573 18.895354977714877 74-75 13.236940495553537 37.47808453560339 29.575315265837222 19.70965970300585 76-77 15.839218505518671 29.583033788641266 29.437666511608242 25.14008119423183 78-79 16.411683627395583 30.455906505632157 32.274674031865395 20.857735835106862 80-81 20.09579484904654 28.530388724302004 29.81680687357087 21.55700955308059 82-83 16.3515768572696 27.56490145962274 35.341458777803595 20.74206290530407 84-85 15.574237975539173 30.759806510213135 32.54367250374939 21.1222830104983 86-87 15.162262156448204 32.29439746300212 30.396405919661735 22.146934460887948 88-89 14.396920172656987 31.73950854270291 27.207792896458834 26.655778388181268 90-91 17.284114220288043 26.67967114225589 29.300355679475015 26.73585895798106 92-93 19.84696140765932 31.201812382501426 29.994613548509747 18.9566126613295 94-95 17.52261553329355 28.88503968610221 31.108834448487283 22.483510332116964 96-97 11.239200692845525 30.790963435499886 35.33512177605036 22.634714095604235 98-99 16.428677045267314 32.522549164571934 27.393802412285336 23.654971377875412 100-101 14.508114045510476 28.58689395499649 30.04018736500795 26.86480463448509 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7496.0 1 4337.5 2 1024.5 3 726.0 4 505.5 5 504.5 6 850.0 7 1671.5 8 1949.5 9 1462.0 10 879.5 11 425.5 12 311.0 13 275.0 14 249.5 15 225.5 16 219.5 17 191.5 18 182.5 19 207.0 20 211.5 21 217.5 22 238.5 23 249.5 24 270.0 25 309.0 26 367.5 27 452.5 28 533.5 29 603.0 30 675.5 31 715.5 32 714.0 33 718.0 34 712.0 35 666.0 36 595.5 37 487.0 38 379.5 39 313.0 40 270.0 41 268.0 42 294.5 43 341.0 44 405.5 45 653.0 46 2084.0 47 7932.5 48 17101.5 49 17287.5 50 9804.5 51 4928.0 52 2106.0 53 693.5 54 250.5 55 123.5 56 93.5 57 85.0 58 74.0 59 61.0 60 60.0 61 54.0 62 40.5 63 35.0 64 34.0 65 25.5 66 20.0 67 26.5 68 32.0 69 26.0 70 20.0 71 17.5 72 13.0 73 11.5 74 9.5 75 8.0 76 6.0 77 3.5 78 3.5 79 2.5 80 1.5 81 1.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4330284531378721 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.002112333917745717 16-17 0.003696584356055005 18-19 5.280834794364293E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.014258253944783592 36-37 0.029044591369003614 38-39 0.013202086985910733 40-41 0.006865085232673582 42-43 0.006865085232673582 44-45 0.003168500876618576 46-47 0.001584250438309288 48-49 0.004752751314927864 50-51 0.0010561669588728586 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0010561669588728586 60-61 0.001584250438309288 62-63 0.002112333917745717 64-65 5.280834794364293E-4 66-67 0.0026404173971821465 68-69 0.0026404173971821465 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.09927969413404872 78-79 0.0026404173971821465 80-81 5.280834794364293E-4 82-83 0.0 84-85 0.0 86-87 0.08660569062757441 88-89 0.41296128091928774 90-91 0.37546735387930125 92-93 0.0 94-95 0.001584250438309288 96-97 0.0 98-99 0.0 100-101 5.280834794364293E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 94682.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.37605880737627 #Duplication Level Percentage of deduplicated Percentage of total 1 81.71650863116946 14.199108595086713 2 8.309020179917335 2.8875604655583955 3 3.099927060539752 1.6159354470754739 4 1.811329929491855 1.2589510149764476 5 0.8935083880379285 0.7762827147715511 6 0.6078288353999514 0.6337001753237151 7 0.5166545100899587 0.6284193405293509 8 0.3768538779479698 0.523858811600938 9 0.23705324580598103 0.3707146025643734 >10 1.7930950644298567 6.288418073129 >50 0.1884269389739849 2.2147821127563847 >100 0.2735229759299781 10.121247966878604 >500 0.042548018477996594 5.333643142307936 >1k 0.11548747872599076 34.19023679263218 >5k 0.01823486506199854 18.95714074480894 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7086 7.483999070573076 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5618 5.9335459749477195 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5245 5.539595699288143 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4043 4.270083014722967 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2767 2.9224139752012 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2142 2.2623096259056634 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2096 2.2137259457975116 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1982 2.0933229124860055 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1964 2.0743119072262943 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1828 1.9306732008195857 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1776 1.875752518958197 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1625 1.7162713081683953 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1511 1.5958682748568893 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1390 1.4680720728332735 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1262 1.3328827020975476 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1240 1.3096470290023448 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1194 1.2610633488941931 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1151 1.2156481696626602 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1138 1.2019179991973132 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1135 1.1987494983206946 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1099 1.1607274878012717 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1029 1.0867958006801717 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 938 0.9906846074227414 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 819 0.8650007393168713 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 738 0.7794512156481697 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 685 0.7234743668279081 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 624 0.6590481823366637 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 623 0.657992015377791 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 623 0.657992015377791 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 499 0.5270273124775565 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 435 0.4594326271096935 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 430 0.4541517923153292 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 411 0.4340846200967449 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 401 0.42352295050801625 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 367 0.38761327390633915 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 361 0.381276272153102 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 310 0.3274117572505862 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 303 0.32001858853847615 RNA PCR Primer, Index 33 (95% over 22bp) GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 296 0.31262541982636616 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 270 0.28516507889567183 No Hit GTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 267 0.28199657801905326 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 247 0.2608732388415961 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 245 0.2587609049238504 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 237 0.25031156925286746 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 225 0.2376375657463932 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 215 0.2270758961576646 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 213 0.2249635622399189 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 201 0.2122895587334446 RNA PCR Primer, Index 33 (95% over 24bp) GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 191 0.201727889144716 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 187 0.19750322130922454 No Hit TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 185 0.19539088739147886 RNA PCR Primer, Index 33 (96% over 29bp) CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 184 0.19433472043260597 No Hit TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 183 0.19327855347373313 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 178 0.18799771867936882 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 166 0.17532371517289452 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 163 0.17215521429627595 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 158 0.16687437950191167 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 156 0.16476204558416593 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 152 0.16053737774867452 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 138 0.1457510403244545 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 133 0.1404702055300902 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 133 0.1404702055300902 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 128 0.1351893707357259 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 124 0.1309647029002345 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 120 0.12674003506474302 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 118 0.12462770114699732 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 117 0.12357153418812446 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 117 0.12357153418812446 No Hit TGATACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 109 0.11512219851714159 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 103 0.10878519676390444 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 102 0.10772902980503159 No Hit ACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 102 0.10772902980503159 No Hit TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 102 0.10772902980503159 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 101 0.1066728628461587 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.280834794364293E-4 0.0 12-13 0.0 0.0 0.0 0.0010561669588728586 0.0 14-15 0.0 0.0 0.0 0.0010561669588728586 0.0 16-17 0.0 0.0 0.0 0.0015842504383092879 0.0 18-19 0.0 0.0 0.0 0.004224667835491434 0.0 20-21 0.0 0.0 0.0 0.008977419150419299 0.0 22-23 0.0 0.0 0.0 0.018482921780275025 0.0 24-25 0.0 0.0 0.0 0.03010075832787647 0.0 26-27 0.0 0.0 0.0 0.06389810101180796 0.0 28-29 0.0 0.0 0.0 0.18324496736444096 0.0 30-31 0.0 0.0 0.0 0.43408462009674487 0.0 32-33 0.0 0.0 0.0 0.7609682938678946 0.0 34-35 0.0 0.0 0.0 1.1839631608964747 0.0 36-37 0.0 0.0 0.0 1.6983164698675566 0.0 38-39 0.0 0.0 0.0 2.384296909655478 0.0 40-41 0.0 0.0 0.0 3.0475697598276335 0.0 42-43 0.0 0.0 0.0 3.6738767664392387 0.0 44-45 0.0 0.0 0.0 4.363025707103779 0.0 46-47 0.0 0.0 0.0 5.051118480809446 0.0 48-49 0.0 0.0 0.0 5.535899114932089 0.0 50-51 0.0 0.0 0.0 6.058701759574154 0.0 52-53 0.0 0.0 0.0 6.551931729367778 0.0 54-55 0.0 0.0 0.0 7.103250881899411 0.0 56-57 0.0 0.0 0.0 7.887454848862508 0.0 58-59 0.0 0.0 0.0 8.426099997887665 0.0 60-61 0.0 0.0 0.0 9.026530914006887 0.0 62-63 0.0 0.0 0.0 9.644388584947508 0.0 64-65 0.0 0.0 0.0 10.197291987917449 0.0 66-67 0.0 0.0 0.0 10.897530681650156 0.0 68-69 0.0 0.0 0.0 11.816924019348978 0.0 70-71 0.0 0.0 0.0 12.64179041422868 0.0 72-73 0.0 0.0 0.0 13.282883758264507 0.0 74-75 0.0 0.0 0.0 14.1859065081008 0.0 76-77 0.0 0.0 0.0 14.813797765150715 0.0 78-79 0.0 0.0 0.0 15.984558839061279 0.0 80-81 0.0 0.0 0.0 17.059208719714412 0.0 82-83 0.0 0.0 0.0 17.85080585538962 0.0 84-85 0.0 0.0 0.0 18.798715700978008 0.0 86-87 0.0 0.0 0.0 19.69804186645825 0.0 88-89 0.0 0.0 0.0 20.65176063032044 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCA 35 2.7420992E-4 54.41623 1 ACCCATG 35 2.785477E-4 54.24375 3 CGCAGAA 335 0.0 49.58851 9 TACCCAT 40 5.38349E-4 47.463284 2 GTAAGCG 65 0.0 43.812263 94-95 GCTTTGC 215 0.0 35.433823 1 TTTGCGT 225 0.0 33.751667 3 CTTTGCG 230 0.0 33.017937 2 GTTGATC 95 0.0 32.47488 94-95 CAGAAAA 340 0.0 32.107517 10-11 TCCGCCC 30 0.003947416 31.658915 80-81 CAAAAGG 40 4.4999606E-4 29.664553 94-95 TTGCGTT 260 0.0 29.208176 4 TGCTTTG 160 6.1618266E-8 26.698097 7 CTTACTT 45 8.960214E-4 26.36849 94-95 GCAGAAA 330 0.0 24.450785 10-11 AAGCAGG 90 1.9476647E-7 23.731642 94-95 AGTACTT 780 0.0 22.210382 12-13 GAGTACT 815 0.0 20.965378 12-13 TACTTTT 830 0.0 20.87241 14-15 >>END_MODULE