##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939056_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 96429 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.223241970776428 33.0 31.0 34.0 30.0 34.0 2 31.13433718072364 33.0 31.0 34.0 27.0 34.0 3 31.08567961920169 33.0 31.0 34.0 27.0 34.0 4 34.557093820323765 37.0 35.0 37.0 32.0 37.0 5 34.62572462640907 37.0 35.0 37.0 32.0 37.0 6 34.832591855147314 37.0 35.0 37.0 32.0 37.0 7 34.82247041864999 37.0 35.0 37.0 32.0 37.0 8 34.85666137780128 37.0 35.0 37.0 32.0 37.0 9 36.2476744547802 39.0 37.0 39.0 32.0 39.0 10-11 36.156934117329854 39.0 37.0 39.0 32.0 39.0 12-13 36.0980669715542 39.0 37.0 39.0 31.5 39.0 14-15 36.971569755986266 40.0 37.0 41.0 31.0 41.0 16-17 36.901051550881995 39.5 37.0 41.0 31.5 41.0 18-19 36.65211191653963 39.0 35.5 41.0 31.5 41.0 20-21 36.4456491304483 39.0 35.0 41.0 31.0 41.0 22-23 36.46730755270717 39.0 35.0 41.0 31.0 41.0 24-25 36.264930674382185 39.0 35.0 41.0 30.5 41.0 26-27 35.973731968598656 38.5 35.0 41.0 30.0 41.0 28-29 35.80682159931141 38.0 35.0 40.5 30.0 41.0 30-31 35.65905484864512 38.0 35.0 41.0 30.0 41.0 32-33 35.256152194879135 38.0 35.0 40.0 29.5 41.0 34-35 34.795673500710365 37.0 35.0 40.0 26.5 41.0 36-37 34.3918530732456 36.5 34.5 40.0 24.5 41.0 38-39 34.10591212187205 36.0 35.0 40.0 24.0 41.0 40-41 34.13627643136401 37.0 35.0 40.0 24.0 41.0 42-43 33.781906895228616 36.0 34.5 40.0 21.5 41.0 44-45 33.38719679764387 37.0 33.0 40.0 16.5 41.0 46-47 32.94011137728277 36.0 33.0 40.0 10.0 41.0 48-49 32.51514585861099 36.0 33.0 40.0 2.0 41.0 50-51 31.2265293635732 35.5 30.5 39.0 2.0 40.0 52-53 31.535938358792478 35.5 32.0 39.0 2.0 40.0 54-55 32.05796492756328 36.0 33.0 40.0 2.0 41.0 56-57 31.740664115566894 35.5 32.5 40.0 2.0 41.0 58-59 31.527517655477087 35.5 32.0 40.0 2.0 41.0 60-61 30.952011324394114 35.0 31.0 39.0 2.0 41.0 62-63 30.67513922160346 35.0 31.0 39.0 2.0 41.0 64-65 30.20814796378683 35.0 30.5 38.5 2.0 40.0 66-67 29.524365076895954 35.0 29.0 37.5 2.0 39.0 68-69 28.939483972663822 35.0 28.5 37.0 2.0 39.0 70-71 28.114737267834364 34.5 27.0 36.0 2.0 38.0 72-73 27.53098652894876 34.0 26.0 35.0 2.0 37.0 74-75 27.050254591461076 34.0 26.0 35.0 2.0 37.0 76-77 26.643105289902415 34.0 25.5 35.0 2.0 36.0 78-79 26.119409098922524 34.0 22.5 35.0 2.0 36.0 80-81 25.54417758143297 33.0 20.0 35.0 2.0 35.5 82-83 25.05450642441589 33.0 14.5 35.0 2.0 35.0 84-85 24.60086177394767 33.0 6.5 35.0 2.0 35.0 86-87 24.3555361976169 33.0 2.0 35.0 2.0 35.0 88-89 23.763369940578045 32.0 2.0 35.0 2.0 35.0 90-91 23.161403727094545 32.0 2.0 35.0 2.0 35.0 92-93 22.624988333385183 31.0 2.0 35.0 2.0 35.0 94-95 21.958083149260077 31.0 2.0 35.0 2.0 35.0 96-97 21.201967250515924 30.0 2.0 34.0 2.0 35.0 98-99 20.586073691524334 29.5 2.0 34.0 2.0 35.0 100-101 18.392319737838204 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.774596749541999 1101 2 0.7113148124920059 1101 3 0.46711746020795886 1101 4 0.6185732997524411 1101 5 0.45199161347527905 1101 6 0.9449613374966148 1101 7 0.966864727568705 1101 8 0.8964732480303468 1101 9 1.385366977455135 1101 10-11 1.170209115422054 1101 12-13 1.3828690743066687 1101 14-15 1.40655662853856 1101 16-17 1.5316556214092714 1101 18-19 1.162762214691206 1101 20-21 1.3652395960067096 1101 22-23 1.6535417773850796 1101 24-25 1.345472627761609 1101 26-27 1.1035380885454344 1101 28-29 1.283983935277142 1101 30-31 1.4732895780254935 1101 32-33 1.5669753225864866 1101 34-35 2.174348223215702 1101 36-37 1.7188661231725604 1101 38-39 1.878480814631935 1101 40-41 1.8551928201983046 1101 42-43 1.7036633975500877 1101 44-45 1.7447400062980023 1101 46-47 1.4219408428191471 1101 48-49 0.8313937289972984 1101 50-51 0.5276867714509486 1101 52-53 0.12200873959035974 1101 54-55 0.36375571225066494 1101 56-57 0.5597920051170995 1101 58-59 0.6351132762858072 1101 60-61 0.0026423731010964957 1101 62-63 -0.5549475375403752 1101 64-65 -0.2212613874378242 1101 66-67 0.050805164919680124 1101 68-69 -0.47423353966842896 1101 70-71 -1.4246843416224095 1101 72-73 -0.894587507646321 1101 74-75 -0.9818348970080777 1101 76-77 -0.5045798943534443 1101 78-79 -0.47243983828582614 1101 80-81 -1.438581908685446 1101 82-83 -1.6216239891690876 1101 84-85 -1.682850110073879 1101 86-87 -1.7847487895671783 1101 88-89 -0.9488074567809797 1101 90-91 -1.3603374159923547 1101 92-93 -1.6100914148537342 1101 94-95 -1.6632646349725349 1101 96-97 -1.34030936543234 1101 98-99 -0.6659818639713428 1101 100-101 -0.5368205525961365 1102 1 0.3134118670087993 1102 2 1.3532964263837322 1102 3 0.6287619954479986 1102 4 1.1552178349924773 1102 5 0.5183858933527006 1102 6 0.688832634738695 1102 7 0.3285858715932193 1102 8 0.7530871397566017 1102 9 0.83914634415585 1102 10-11 0.863263252296413 1102 12-13 0.8271361836018656 1102 14-15 1.492230785841933 1102 16-17 1.1386525570578883 1102 18-19 0.7872514894613758 1102 20-21 1.1472876041783167 1102 22-23 1.4207787538917174 1102 24-25 1.1861263560557873 1102 26-27 0.9666637269519711 1102 28-29 0.6136570711300564 1102 30-31 0.8364152164320302 1102 32-33 0.8155069875507408 1102 34-35 0.8425683457897577 1102 36-37 0.18860565330535906 1102 38-39 0.35543433863602303 1102 40-41 1.1242811756630644 1102 42-43 1.3555403127696977 1102 44-45 1.152617942968071 1102 46-47 0.7887053984881973 1102 48-49 0.619698121234272 1102 50-51 1.104167874617442 1102 52-53 0.7555633871899516 1102 54-55 1.0167179185836517 1102 56-57 0.88837984985663 1102 58-59 0.6496689453358613 1102 60-61 0.5707603506291861 1102 62-63 1.4431372428477793 1102 64-65 1.7728014317654406 1102 66-67 1.9982643069012944 1102 68-69 2.0023369404132865 1102 70-71 2.589232920890357 1102 72-73 2.2044293462862647 1102 74-75 2.275251415555765 1102 76-77 1.9263445183125398 1102 78-79 1.452802756198345 1102 80-81 0.756195925839581 1102 82-83 1.024890311137348 1102 84-85 0.9574716468209097 1102 86-87 0.3482951328025905 1102 88-89 0.5808784472026751 1102 90-91 0.5901860773682195 1102 92-93 0.9498549589971361 1102 94-95 1.8655274998589277 1102 96-97 1.8850481422285377 1102 98-99 2.0695901482860606 1102 100-101 2.2215425730422673 1103 1 0.4370073726267769 1103 2 0.8364424937994634 1103 3 0.9770766021895732 1103 4 0.7731953630823654 1103 5 1.0689476911055138 1103 6 0.913551735862292 1103 7 0.7892600288965923 1103 8 0.6744354543633406 1103 9 1.0301575801109024 1103 10-11 0.9587688702739428 1103 12-13 0.9956755094445597 1103 14-15 1.3293094375273213 1103 16-17 0.9420233435747534 1103 18-19 0.7872514894613758 1103 20-21 1.0180741210322424 1103 22-23 1.1286439224310456 1103 24-25 1.4220814122355634 1103 26-27 1.6071131651542245 1103 28-29 1.7821963969727506 1103 30-31 2.021808474859 1103 32-33 1.708765414517032 1103 34-35 1.6178492446661608 1103 36-37 1.952650597125583 1103 38-39 1.962175911669732 1103 40-41 2.1916969060001463 1103 42-43 2.2375627846798096 1103 44-45 2.6189100777995407 1103 46-47 3.620166072645496 1103 48-49 3.9455408178634883 1103 50-51 3.8064150656286806 1103 52-53 3.542080241122534 1103 54-55 3.606605559033092 1103 56-57 3.6805146813173018 1103 58-59 3.256410518369563 1103 60-61 2.964018777595477 1103 62-63 2.7465080293646302 1103 64-65 2.845835139630612 1103 66-67 2.1724216102720817 1103 68-69 2.7551459291773313 1103 70-71 2.707210448980245 1103 72-73 2.063979908084015 1103 74-75 1.7078356852186865 1103 76-77 2.3027490126945622 1103 78-79 2.4359488236140727 1103 80-81 1.7505779483114914 1103 82-83 1.9406206482159973 1103 84-85 2.412527826596193 1103 86-87 2.235935582240792 1103 88-89 2.580878447202675 1103 90-91 1.477826526806421 1103 92-93 2.522888666862304 1103 94-95 2.719460084128592 1103 96-97 2.21650881638584 1103 98-99 1.4740845303085344 1103 100-101 2.3507560561883345 1104 1 -0.003415935447431906 1104 2 -0.08255410928389395 1104 3 0.6441787709902016 1104 4 1.0194718198370012 1104 5 0.012245313263861135 1104 6 0.3471812106467169 1104 7 0.42748840621564455 1104 8 0.5188305418992698 1104 9 0.6787073580048144 1104 10-11 0.04388776235651193 1104 12-13 0.17845575506776612 1104 14-15 0.22923104714320885 1104 16-17 -0.0037566407471771868 1104 18-19 -0.472808609833109 1104 20-21 -0.36388302555776875 1104 22-23 -0.13794870887284816 1104 24-25 -0.4351827633588954 1104 26-27 -0.08990800024948697 1104 28-29 -0.6018511598602814 1104 30-31 -0.19108674332757403 1104 32-33 -0.2032413793162533 1104 34-35 0.5296600625391648 1104 36-37 0.7233587235953962 1104 38-39 0.44362352076301903 1104 40-41 -0.027338892275260207 1104 42-43 -0.2909190548812006 1104 44-45 -1.0238910719260943 1104 46-47 -0.5988044003098238 1104 48-49 -0.9106781526094707 1104 50-51 -0.0957276048704081 1104 52-53 -0.7997671589297255 1104 54-55 -1.0342358310897168 1104 56-57 -1.5214712084135584 1104 58-59 -1.319432178259646 1104 60-61 -1.452299487364023 1104 62-63 -1.5626113853205048 1104 64-65 -1.4880425713701726 1104 66-67 -1.1892846870887759 1104 68-69 -1.3210887193724474 1104 70-71 -1.2848851872883706 1104 72-73 -1.1867386704370197 1104 74-75 -1.1476647067332344 1104 76-77 -1.1988187949876945 1104 78-79 -1.5560814873344526 1104 80-81 -0.9114941708418947 1104 82-83 -1.1748745177103146 1104 84-85 -0.5895625836468206 1104 86-87 -0.6357001637743629 1104 88-89 0.04788916055517234 1104 90-91 -0.7928162743433056 1104 92-93 -0.7109106537543681 1104 94-95 -1.2461134721818397 1104 96-97 -0.06837490454439177 1104 98-99 -0.08960765678317983 1104 100-101 -0.08901399868493343 1105 1 0.7846977596430094 1105 2 0.5254562266334197 1105 3 0.7979255410160384 1105 4 0.35771471389384857 1105 5 0.10552696701063269 1105 6 0.8828401253754024 1105 7 0.982268767972748 1105 8 0.8462207227778222 1105 9 0.5606195027076595 1105 10-11 0.5131384083513453 1105 12-13 0.41708624602383537 1105 14-15 0.4871121840941157 1105 16-17 1.1321606719143276 1105 18-19 1.2367521136810993 1105 20-21 0.8675123232794419 1105 22-23 0.8320771309204318 1105 24-25 0.9914322237212119 1105 26-27 0.827275462282806 1105 28-29 0.9693374706306841 1105 30-31 1.2303602850962037 1105 32-33 0.9974046155157836 1105 34-35 1.05149468786216 1105 36-37 0.5979065272129702 1105 38-39 0.6384555621066923 1105 40-41 0.48183423433972195 1105 42-43 0.5818199632066552 1105 44-45 0.08476525882326058 1105 46-47 0.06954185292016035 1105 48-49 0.5627068603104277 1105 50-51 0.4622827310469084 1105 52-53 0.24726126484288358 1105 54-55 0.710725409220359 1105 56-57 0.9318879647130593 1105 58-59 0.9139011550736882 1105 60-61 0.8595868175455408 1105 62-63 1.2412645836717466 1105 64-65 0.7169961883197509 1105 66-67 0.7580021346166497 1105 68-69 1.162761409826519 1105 70-71 0.4583964664583995 1105 72-73 1.0068013812425676 1105 74-75 1.3877358100626296 1105 76-77 1.686581721808171 1105 78-79 2.218595515249529 1105 80-81 1.7522009195973816 1105 82-83 1.2125931825480833 1105 84-85 1.13911958689582 1105 86-87 1.0228269680085837 1105 88-89 0.6210157755422507 1105 90-91 0.5084757153207775 1105 92-93 1.0306914134290963 1105 94-95 0.6381242539163523 1105 96-97 0.39984214971917353 1105 98-99 0.7499272269377464 1105 100-101 1.613053184777602 1106 1 0.009142204087453365 1106 2 -0.15232155114435741 1106 3 0.27348109657159725 1106 4 -0.1700630638839229 1106 5 0.09108252256618243 1106 6 0.04950679204206665 1106 7 -0.039953454249477716 1106 8 -0.2548903883332869 1106 9 -0.6182693861812325 1106 10-11 -0.09797270275976899 1106 12-13 0.2476418015793982 1106 14-15 -0.12844337146143658 1106 16-17 0.24438289413654246 1106 18-19 0.08230766923666266 1106 20-21 -0.24748767672056715 1106 22-23 -0.18236731352401137 1106 24-25 -0.5663455540565749 1106 26-27 -0.1732800932727443 1106 28-29 -0.245106973813769 1106 30-31 0.04147139620731366 1106 32-33 0.7412935044046733 1106 34-35 0.27593913230661116 1106 36-37 -0.2943156950092529 1106 38-39 -0.35265554900442453 1106 40-41 0.5090564565619431 1106 42-43 0.5607088520955443 1106 44-45 0.7058763699343658 1106 46-47 1.2739862973646012 1106 48-49 0.37548463808821 1106 50-51 0.07450495326912687 1106 52-53 0.09837237595399984 1106 54-55 0.012392075887021292 1106 56-57 0.07911018693527794 1106 58-59 0.11556782174035263 1106 60-61 -0.3248576268989041 1106 62-63 -0.2404020829949225 1106 64-65 0.17532952165307947 1106 66-67 0.04966880128331752 1106 68-69 0.31553918760429767 1106 70-71 -0.3260479779860468 1106 72-73 0.20791249235367815 1106 74-75 0.21384692117374016 1106 76-77 0.32269283291928375 1106 78-79 0.4791510708050808 1106 80-81 0.2516453640418277 1106 82-83 0.42814873810363707 1106 84-85 0.5507862535624852 1106 86-87 0.8361603013419163 1106 88-89 1.4932379977644743 1106 90-91 1.406253493098557 1106 92-93 2.06013585787354 1106 94-95 2.574235365027466 1106 96-97 2.9015088163858422 1106 98-99 3.5249272269377485 1106 100-101 4.057497629222045 1107 1 0.6054308638812671 1107 2 0.16726607772162083 1107 3 0.4514192409014939 1107 4 0.421174049518136 1107 5 0.2579897390610313 1107 6 0.6031150394647469 1107 7 0.28375788595671025 1107 8 0.17634672506877536 1107 9 0.36966875814866285 1107 10-11 0.08914069930208512 1107 12-13 0.5051160283835188 1107 14-15 0.6449586903942262 1107 16-17 0.4719086673324213 1107 18-19 -0.07212532045406306 1107 20-21 0.2767906737948991 1107 22-23 0.31392134626980095 1107 24-25 0.885303930479516 1107 26-27 1.004194133531378 1107 28-29 1.2228311705161303 1107 30-31 0.96157448899082 1107 32-33 0.5419636074974576 1107 34-35 1.037691709626202 1107 36-37 1.5205296658154879 1107 38-39 1.224251667490421 1107 40-41 1.5669430545000935 1107 42-43 1.5866882335388368 1107 44-45 1.2417526585941587 1107 46-47 1.5742955757151194 1107 48-49 1.4853299989129454 1107 50-51 1.9066699017227329 1107 52-53 1.915588870799354 1107 54-55 1.6887322820725927 1107 56-57 1.499986475595069 1107 58-59 1.8274750382351996 1107 60-61 0.8898846411423342 1107 62-63 1.1148041025720907 1107 64-65 1.4410511711376195 1107 66-67 1.20972034767507 1107 68-69 0.75497217729502 1107 70-71 0.571683980776843 1107 72-73 -0.154252456099929 1107 74-75 -0.42589534686749175 1107 76-77 -0.29148242481267417 1107 78-79 -0.31657057867945326 1107 80-81 0.3708721681655369 1107 82-83 0.3150559545984848 1107 84-85 -0.6168941588086518 1107 86-87 -0.7015716574209705 1107 88-89 -1.3995455073901653 1107 90-91 -1.1091073316437132 1107 92-93 -0.8624930081058437 1107 94-95 -0.08530071744676349 1107 96-97 0.45877685762295073 1107 98-99 1.372092175391355 1107 100-101 1.6172914436550307 1108 1 0.1501948356664009 1108 2 -0.4470583932496197 1108 3 -0.7780978507968257 1108 4 -0.3253262217786599 1108 5 -0.8599701090127638 1108 6 -0.12417741848425123 1108 7 -0.47153240161789256 1108 8 -0.45436407254381095 1108 9 -0.9651114914443895 1108 10-11 0.10992203408233792 1108 12-13 -0.5299897773679731 1108 14-15 -0.036864424093018044 1108 16-17 -0.39824868481082376 1108 18-19 -0.668218646552809 1108 20-21 -0.3851192556679308 1108 22-23 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-2.582899856412176 2316 94-95 -2.94005034925825 2316 96-97 -2.6495626121855906 2316 98-99 -3.2759656302051106 2316 100-101 -3.029109513635099 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1472.0 3 269.0 4 236.0 5 237.0 6 219.0 7 238.0 8 263.0 9 317.0 10 390.0 11 461.0 12 647.0 13 921.0 14 1024.0 15 1153.0 16 1419.0 17 1663.0 18 1383.0 19 1009.0 20 1065.0 21 1176.0 22 1240.0 23 1333.0 24 1472.0 25 1603.0 26 1800.0 27 2037.0 28 2325.0 29 2554.0 30 3023.0 31 3425.0 32 4042.0 33 5022.0 34 6877.0 35 13768.0 36 10508.0 37 13216.0 38 6585.0 39 37.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.067049908001295 20.75948314396499 18.42157239830764 10.751894549726083 2 14.316401183002128 30.60654802054688 30.959373216416747 24.117677580034243 3 29.877340085509115 27.178199327549706 34.07911668257856 8.865343904362625 4 10.412320848511266 21.170231534813247 42.4556596822234 25.96178793445209 5 7.350636681576189 43.36180325657178 35.55869197480256 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40.102374846229225 16.898625966931466 52-53 16.563540922762556 35.70270594306625 28.791109118678875 18.94264401549232 54-55 13.28572917752528 28.706348379026377 33.60471176899823 24.403210674450122 56-57 19.947786937354746 26.865757193626045 31.65247569122383 21.533980177795378 58-59 25.13235784558946 26.79464732366183 30.840941303985325 17.232053526763384 60-61 17.3950634645333 30.08536591717579 37.603712558008716 14.915858060282192 62-63 13.95833659073023 30.5179000474277 36.336392680498875 19.187370681343193 64-65 13.556839152964727 36.42178686490601 31.938985100770505 18.08238888135876 66-67 17.65883567428911 28.819303239799375 28.631609680816677 24.890251405094837 68-69 14.41836942183663 28.953528991590066 33.264858157322585 23.36324342925072 70-71 21.907944816942827 29.259429191128167 29.260471954869182 19.572154037059825 72-73 18.820640613921675 24.244067231096466 38.643804479292655 18.291487675689204 74-75 14.52589105581708 35.44048419636853 31.419589934470842 18.61403481334355 76-77 17.003742585198545 27.615900253328192 30.94617557832845 24.434181583144817 78-79 18.102226298674225 28.27013674643542 34.06883181856083 19.558805136329525 80-81 21.946363986950583 26.3672180357087 31.557278800954734 20.12913917638598 82-83 18.248958892543115 25.161703924154217 37.191641952851775 19.397695230450896 84-85 17.93242567032234 28.76235352987782 33.86430924481881 19.440911554981028 86-87 18.12188270366336 29.844219874604555 31.925283911754125 20.108613509977953 88-89 17.391032390563264 29.38242131709809 28.516101456376468 24.71044483596218 90-91 20.3654372474912 24.18610712889352 30.45901212042226 24.989443503193016 92-93 23.324922319771442 27.854357391612623 31.66798740433341 17.152732884282525 94-95 21.175912458228417 25.869969814979903 32.27763962526783 20.67647810152385 96-97 15.987384005838805 26.81003023668022 36.3366697945991 20.865915962881868 98-99 20.70818974730597 29.17424783513109 28.81347979542575 21.304082622137184 100-101 19.376563803169308 24.05025020850709 31.676396997497914 24.896788990825687 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8071.0 1 4571.5 2 956.0 3 710.0 4 494.5 5 513.5 6 900.0 7 1596.0 8 1858.0 9 1432.5 10 822.5 11 395.0 12 298.5 13 259.0 14 240.5 15 255.0 16 299.0 17 303.0 18 237.5 19 200.5 20 204.5 21 222.5 22 250.5 23 281.0 24 286.5 25 307.0 26 368.0 27 452.0 28 503.5 29 552.0 30 599.5 31 625.5 32 648.5 33 666.0 34 675.0 35 647.5 36 598.5 37 534.5 38 464.0 39 372.5 40 299.5 41 279.5 42 242.5 43 226.0 44 265.0 45 522.5 46 2250.5 47 7729.5 48 16609.0 49 16888.5 50 9085.0 51 4331.0 52 2253.0 53 1527.0 54 1055.5 55 654.0 56 444.5 57 325.0 58 243.5 59 185.0 60 148.5 61 145.0 62 136.0 63 120.0 64 111.5 65 98.0 66 83.5 67 84.5 68 75.5 69 62.5 70 53.5 71 45.0 72 47.0 73 37.0 74 26.0 75 20.0 76 17.0 77 17.5 78 12.5 79 8.5 80 8.0 81 6.5 82 4.5 83 3.0 84 0.5 85 0.5 86 0.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2405915232969335 2 0.0663700753922575 3 0.06740710782026155 4 0.0746663348162897 5 0.07259226996028166 6 0.06014788082423338 7 0.0663700753922575 8 0.06429601053624948 9 0.06844414024826556 10-11 0.06222194568024142 12-13 0.06688859160625953 14-15 0.07570336724429373 16-17 0.0663700753922575 18-19 0.07311078617428368 20-21 0.07725891588629977 22-23 0.08918478880834604 24-25 0.10214769415839632 26-27 0.10370324280040237 28-29 0.09436995094836616 30-31 0.08866627259434402 32-33 0.08399962666832592 34-35 0.33288740938929157 36-37 0.5278495058540481 38-39 0.5097014383639776 40-41 0.48170156280786897 42-43 0.5014051789399455 44-45 0.5278495058540481 46-47 0.5138495680759937 48-49 0.5335531842080702 50-51 0.52577544099804 52-53 0.5294050544960541 54-55 0.5174791815740079 56-57 0.4936274357299153 58-59 0.4946644681579193 60-61 0.44644246025573214 62-63 0.5117755032199858 64-65 0.5371827977060842 66-67 0.5475531219861245 68-69 0.5501457030561345 70-71 0.5496271868421326 72-73 0.5402938949900964 74-75 0.5366642814920822 76-77 0.524738408570036 78-79 0.5029607275819515 80-81 0.5039977600099556 82-83 0.5159236329320017 84-85 0.5237013761420319 86-87 0.5107384707919816 88-89 0.524738408570036 90-91 0.5356272490640782 92-93 0.5423679598461043 94-95 0.5397753787760943 96-97 0.5382198301340884 98-99 0.5418494436321024 100-101 0.5278495058540481 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 96429.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.01753621835755 #Duplication Level Percentage of deduplicated Percentage of total 1 85.18280949326491 17.903327837061465 2 6.828835052055064 2.8705057607151376 3 2.506537721419056 1.5804374202781322 4 1.287807766319633 1.0826618548362006 5 0.8388019933882666 0.88147756380342 6 0.5772931366260423 0.7279967644588247 7 0.3947303498297725 0.5807381596822533 8 0.2269699511521192 0.38162793350548074 9 0.24670646864360782 0.46666459260181065 >10 1.4111610006414368 5.892418255918862 >50 0.157892139931909 2.2513974011967353 >100 0.19736517491488625 8.465295709796845 >500 0.0345389056101051 4.927978097875121 >1k 0.09374845808457098 33.44740690041378 >5k 0.01480238811861647 18.540065747855934 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6833 7.086042580551494 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5632 5.8405666345186615 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5413 5.61345653278578 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4391 4.553609391365668 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2843 2.9482831928154396 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2161 2.241027076916695 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2004 2.0782129857200635 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1862 1.9309543809434921 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1855 1.923695153947464 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1783 1.8490288191311741 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1745 1.8096215868670213 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1596 1.6551037550944219 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1528 1.584585549990148 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1396 1.447697269493617 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1259 1.305623826857066 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1256 1.3025127295730539 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1251 1.2973275674330336 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1175 1.2185131029047278 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1061 1.100291406112269 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1047 1.0857729521202129 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1028 1.0660693359881364 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1012 1.049476817140072 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 885 0.9177736987835609 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 813 0.8431073639672713 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 674 0.6989598564747119 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 654 0.6782192079146315 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 630 0.6533304296425348 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 593 0.6149602298063861 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 503 0.5216273112860239 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 473 0.4905163384459032 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 422 0.437627684617698 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 419 0.4345165873336859 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 413 0.42829439276566184 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 386 0.4002945172095531 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 294 0.30488753383318296 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 284 0.2945172095531427 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 277 0.28725798255711454 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 266 0.27585062584907033 No Hit GTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 250 0.2592581070010059 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 245 0.2540729448609858 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 242 0.2509618475769737 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 240 0.24888778272096568 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 229 0.2374804260129214 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 216 0.2239990044488691 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 206 0.21362868016882888 No Hit TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 186 0.1928880316087484 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 172 0.1783695776166921 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 171 0.17733254518868805 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 166 0.17214738304866792 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 159 0.16488815605263976 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 157 0.16281409119663173 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 157 0.16281409119663173 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 156 0.1617770587686277 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 148 0.15348079934459552 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 136 0.1410364102085472 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 133 0.13792531292453517 No Hit TGATACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 131 0.1358512480685271 No Hit ACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 130 0.1348142156405231 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 127 0.13170311835651102 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 127 0.13170311835651102 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 127 0.13170311835651102 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 126 0.13066608592850698 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 125 0.12962905350050294 No Hit CCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 124 0.12859202107249895 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 111 0.11511059950844664 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 111 0.11511059950844664 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 108 0.11199950222443456 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 108 0.11199950222443456 No Hit TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 105 0.10888840494042248 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 98 0.10162917794439433 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0010370324280040237 0.0 12-13 0.0 0.0 0.0 0.0010370324280040237 0.0 14-15 0.0 0.0 0.0 0.0015555486420060355 0.0 16-17 0.0 0.0 0.0 0.003111097284012071 0.0 18-19 0.0 0.0 0.0 0.005185162140020119 0.0 20-21 0.0 0.0 0.0 0.008814775638034202 0.0 22-23 0.0 0.0 0.0010370324280040237 0.01711103506206639 0.0 24-25 0.0 0.0 0.0010370324280040237 0.03007394041211669 0.0 26-27 0.0 0.0 0.0010370324280040237 0.061703429466239405 0.0 28-29 0.0 0.0 0.0010370324280040237 0.1705918344066619 0.0 30-31 0.0 0.0 0.0010370324280040237 0.4070352279915793 0.0 32-33 0.0 0.0 0.0010370324280040237 0.7710336102209916 0.0 34-35 0.0 0.0 0.0010370324280040237 1.2185131029047278 0.0 36-37 0.0 0.0 0.0010370324280040237 1.7059183440666188 0.0 38-39 0.0 0.0 0.0010370324280040237 2.3820634871252424 0.0 40-41 0.0 0.0 0.0010370324280040237 3.035912432981779 0.0 42-43 0.0 0.0 0.0010370324280040237 3.691835443694324 0.0 44-45 0.0 0.0 0.0010370324280040237 4.397536010951063 0.0 46-47 0.0 0.0 0.0010370324280040237 5.125532775409887 0.0 48-49 0.0 0.0 0.0010370324280040237 5.660641508259964 0.0 50-51 0.0 0.0 0.0010370324280040237 6.219083470740131 0.0 52-53 0.0 0.0 0.0010370324280040237 6.717377552396064 0.0 54-55 0.0 0.0 0.0010370324280040237 7.2426344771801014 0.0 56-57 0.0 0.0 0.0010370324280040237 8.011075506331082 0.0 58-59 0.0 0.0 0.0010370324280040237 8.524406558193075 0.0 60-61 0.0 0.0 0.0010370324280040237 9.102033620591316 0.0 62-63 0.0 0.0 0.0010370324280040237 9.680179199203558 0.0 64-65 0.0 0.0 0.0010370324280040237 10.276472845305872 0.0 66-67 0.0 0.0 0.0010370324280040237 10.983728961204616 0.0 68-69 0.0 0.0 0.0010370324280040237 11.831502971097906 0.0 70-71 0.0 0.0 0.0010370324280040237 12.673054786423172 0.0 72-73 0.0 0.0 0.0010370324280040237 13.327940764707712 0.0 74-75 0.0 0.0 0.0010370324280040237 14.259195885055327 0.0 76-77 0.0 0.0 0.0010370324280040237 14.879859793215735 0.0 78-79 0.0 0.0 0.0010370324280040237 16.104595090688484 0.0 80-81 0.0 0.0 0.0010370324280040237 17.161331134824586 0.0 82-83 0.0 0.0 0.0010370324280040237 17.896068610065434 0.0 84-85 0.0 0.0 0.0010370324280040237 18.86102728432318 0.0 86-87 0.0 0.0 0.0010370324280040237 19.66213483495629 0.0 88-89 0.0 0.0 0.0010370324280040237 20.671685903618204 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTTTGC 135 3.092282E-10 35.10318 1 TTTGCGT 135 3.1468517E-10 35.04853 3 CTTTGCG 145 6.7484507E-10 32.63139 2 CGCAGAA 475 0.0 31.875713 9 CAGAAGC 30 0.0040186914 31.54368 10-11 AAGCAGG 95 3.8380676E-10 27.536089 94-95 TTGCGTT 190 4.0199666E-10 27.393194 4 CGTATCA 35 0.008321044 27.178476 94-95 CAGAAAA 405 0.0 24.533972 10-11 GTCGCCG 65 2.8930538E-4 21.963305 90-91 GCCGTAT 55 0.002842665 21.624886 92-93 AGTACTT 675 0.0 20.678633 12-13 GGTCGCC 70 4.7721068E-4 20.394497 88-89 GAAAAAA 615 0.0 19.233948 12-13 GAGTACT 695 0.0 18.381567 12-13 GGGCTTG 235 2.5728732E-6 18.120836 9 TACTTTT 800 0.0 17.447596 14-15 AGAAAAA 505 0.0 17.333406 12-13 TGGGCTT 250 1.490771E-7 17.033587 8 TGGTCGC 85 0.0017585405 16.79547 88-89 >>END_MODULE