##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939056_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 96429 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.836532578373724 31.0 31.0 34.0 30.0 34.0 2 32.006605896566384 33.0 31.0 34.0 30.0 34.0 3 32.03053023468044 33.0 31.0 34.0 30.0 34.0 4 35.63187422870713 37.0 35.0 37.0 33.0 37.0 5 35.57660040029452 37.0 35.0 37.0 33.0 37.0 6 35.712949423928485 37.0 35.0 37.0 33.0 37.0 7 35.69342210330917 37.0 35.0 37.0 33.0 37.0 8 35.74607223967894 37.0 35.0 37.0 33.0 37.0 9 37.24217818291178 39.0 37.0 39.0 33.0 39.0 10-11 37.15829262981053 39.0 37.0 39.0 33.0 39.0 12-13 37.156918561843426 39.0 37.0 39.0 33.0 39.0 14-15 38.03383836812577 40.0 37.0 41.0 33.0 41.0 16-17 37.999953333540745 39.5 37.0 41.0 33.0 41.0 18-19 37.8417903327837 40.0 36.0 41.0 33.0 41.0 20-21 37.74560038992419 40.0 35.5 41.0 33.0 41.0 22-23 37.62327722987898 39.5 35.0 41.0 33.0 41.0 24-25 37.41338186645096 39.0 35.0 41.0 32.5 41.0 26-27 37.05886196061351 38.5 35.0 41.0 32.5 41.0 28-29 36.825011148098596 38.0 35.0 41.0 31.5 41.0 30-31 36.69057026413216 38.0 35.0 41.0 32.0 41.0 32-33 36.22834935548435 38.0 35.0 40.0 31.0 41.0 34-35 35.870536871687975 38.0 35.0 40.0 30.0 41.0 36-37 35.63803938649161 37.5 35.0 40.0 30.0 41.0 38-39 35.36576652251916 37.0 35.0 40.0 30.0 41.0 40-41 35.40931151417105 38.0 35.0 40.0 30.0 41.0 42-43 35.02696802829024 38.0 35.0 40.0 27.5 41.0 44-45 34.66787999460743 37.5 35.0 40.0 24.5 41.0 46-47 34.25135073473747 37.0 34.0 40.0 23.5 41.0 48-49 33.77961505356272 37.0 33.0 40.0 22.5 41.0 50-51 33.397053790872036 37.0 33.0 40.0 20.0 41.0 52-53 33.32638521606571 37.0 33.0 40.0 20.0 41.0 54-55 33.168066660444474 37.0 33.0 40.0 17.0 41.0 56-57 32.600436590652194 35.5 32.5 40.0 10.5 41.0 58-59 32.2492559292329 36.0 32.0 39.0 4.5 41.0 60-61 31.41524333965923 35.0 31.0 39.0 2.0 40.0 62-63 31.166848147341568 35.0 31.0 39.0 2.0 40.0 64-65 30.93576621140943 35.0 31.0 38.5 2.0 39.5 66-67 30.16221779755053 35.0 30.0 37.5 2.0 39.0 68-69 29.535601323253378 35.0 29.0 37.0 2.0 39.0 70-71 28.796503126652773 34.5 28.5 35.5 2.0 38.0 72-73 28.173241452260214 34.0 27.0 35.0 2.0 37.0 74-75 27.854203610946914 34.0 26.5 35.0 2.0 37.0 76-77 26.014388824938557 31.5 25.0 34.0 2.0 35.0 78-79 26.951824658557072 33.0 26.0 35.0 2.0 35.0 80-81 26.821360793952028 33.0 26.0 35.0 2.0 35.0 82-83 26.476516400667848 33.0 25.0 35.0 2.0 35.0 84-85 26.06755747752232 33.0 24.5 35.0 2.0 35.0 86-87 25.759413661865207 33.0 23.0 35.0 2.0 35.0 88-89 25.12642980846011 32.0 19.0 35.0 2.0 35.0 90-91 24.610438768420288 32.0 16.5 34.0 2.0 35.0 92-93 24.293366103558057 32.0 9.0 34.0 2.0 35.0 94-95 23.818187474722336 31.0 2.0 34.0 2.0 35.0 96-97 23.10035881322009 31.0 2.0 34.0 2.0 35.0 98-99 22.32747410011511 31.0 2.0 34.0 2.0 35.0 100-101 18.339602194360616 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5970589037219192 1101 2 0.6175152155596564 1101 3 0.3540507076840669 1101 4 0.3979099655407765 1101 5 0.4417185019443082 1101 6 0.4343636219257405 1101 7 0.5310855746830114 1101 8 0.4121727444457264 1101 9 0.7336879578033475 1101 10-11 0.9254310326810113 1101 12-13 0.7819465241380215 1101 14-15 0.9529208728459864 1101 16-17 0.9147002512895241 1101 18-19 0.9587187928249676 1101 20-21 0.6491096215696714 1101 22-23 0.814122565355099 1101 24-25 0.5868064055273479 1101 26-27 0.5664068786194676 1101 28-29 0.26627512494396655 1101 30-31 0.04791194179801295 1101 32-33 -0.2109834180144574 1101 34-35 -0.004199621270352338 1101 36-37 0.32319295941699266 1101 38-39 0.5785150912620978 1101 40-41 1.166529858053238 1101 42-43 -0.039489259024414025 1101 44-45 -0.11999812501147744 1101 46-47 0.5352390945814918 1101 48-49 -0.3021110292779525 1101 50-51 -0.08195261556376465 1101 52-53 -0.1316466272097756 1101 54-55 -1.001326935912786 1101 56-57 -1.0502995618012463 1101 58-59 -1.0904475583225235 1101 60-61 -0.3269017392318112 1101 62-63 0.11141980408663699 1101 64-65 -0.1901451904600755 1101 66-67 -0.032898594117732216 1101 68-69 0.24907496868619106 1101 70-71 0.3577304756318469 1101 72-73 0.40224969063215354 1101 74-75 0.17525853276281111 1101 76-77 -0.14942543522851182 1101 78-79 0.3950088917943795 1101 80-81 0.9688510731507236 1101 82-83 0.3825507761705147 1101 84-85 0.22009358091539255 1101 86-87 0.3601067970135077 1101 88-89 0.7796037011297621 1101 90-91 1.5054428809353126 1101 92-93 1.4042557570920415 1101 94-95 0.5447800517513386 1101 96-97 0.9170273159228337 1101 98-99 0.6420275158356183 1101 100-101 0.2856596896927819 1102 1 -0.3067970717632704 1102 2 0.10449172219908576 1102 3 0.07940821534494091 1102 4 0.19489668668479965 1102 5 0.0815295336092774 1102 6 0.21909293755393833 1102 7 -0.01066621285529834 1102 8 -0.3088435987616265 1102 9 0.2842497555561536 1102 10-11 0.39651121654208765 1102 12-13 0.0966809470185126 1102 14-15 0.548298809516055 1102 16-17 0.5055455015448871 1102 18-19 -0.062093260290453145 1102 20-21 -0.1198510525876273 1102 22-23 -0.43306844588085625 1102 24-25 -0.2624019703664189 1102 26-27 -0.30054919901076715 1102 28-29 -0.34250934900905605 1102 30-31 -0.5066718171396829 1102 32-33 -0.10583786132395545 1102 34-35 0.1131395002822515 1102 36-37 0.1305857071187333 1102 38-39 0.13935523426516028 1102 40-41 0.2653551900246356 1102 42-43 -0.5517466645402536 1102 44-45 -0.8237136510584548 1102 46-47 -0.27240901573516396 1102 48-49 -0.4385385062953162 1102 50-51 0.0694774150797528 1102 52-53 0.010909552565507852 1102 54-55 0.5855601938114177 1102 56-57 0.20621218487904258 1102 58-59 0.1589140351401923 1102 60-61 -0.531638715738449 1102 62-63 -0.25215271889599933 1102 64-65 -0.7278111761597685 1102 66-67 -0.7825155501953596 1102 68-69 0.1320550504022293 1102 70-71 0.9733458995337898 1102 72-73 1.771950916372706 1102 74-75 0.9334939770937609 1102 76-77 0.7851378946999858 1102 78-79 0.1051084832141953 1102 80-81 1.0213919311691093 1102 82-83 0.5920758017067769 1102 84-85 0.6835128863290798 1102 86-87 0.01096226177346793 1102 88-89 0.123896346686454 1102 90-91 0.25167628236534156 1102 92-93 0.368249628389286 1102 94-95 0.44467790670537255 1102 96-97 0.039026805197604375 1102 98-99 0.6471986087876083 1102 100-101 0.6483384435232225 1103 1 0.378596186663696 1103 2 0.21685127276088423 1103 3 0.14682394568202284 1103 4 0.2398405069095162 1103 5 0.2388329043957924 1103 6 0.07302552182359534 1103 7 0.2814686186053805 1103 8 0.657448536069829 1103 9 0.38537335106177295 1103 10-11 0.3908932390139981 1103 12-13 0.6865685874679528 1103 14-15 0.25616397805537616 1103 16-17 0.7358825801965736 1103 18-19 0.9603786498219051 1103 20-21 1.0037444530303432 1103 22-23 1.078167509175323 1103 24-25 0.5578227487347007 1103 26-27 1.0477654077308145 1103 28-29 1.2417603139122946 1103 30-31 1.5495079581412199 1103 32-33 1.2593306780018807 1103 34-35 1.5906675901698861 1103 36-37 1.1699115498153603 1103 38-39 1.6056473690966229 1103 40-41 1.7541192349684565 1103 42-43 2.257242099504694 1103 44-45 2.507747023098844 1103 46-47 1.834332557298552 1103 48-49 2.190674976850751 1103 50-51 2.738016740922447 1103 52-53 2.6850668559362916 1103 54-55 2.74286356459794 1103 56-57 1.9421672410588187 1103 58-59 2.4566668441289607 1103 60-61 2.7492601606660436 1103 62-63 3.3040270563848964 1103 64-65 2.7609528687840488 1103 66-67 3.1275968093552002 1103 68-69 3.4635157245595316 1103 70-71 2.9901998321180585 1103 72-73 3.3562205792940496 1103 74-75 3.3548422917005034 1103 76-77 2.807609804812344 1103 78-79 3.54331073040521 1103 80-81 3.6899312570118035 1103 82-83 3.9291544533921687 1103 84-85 4.228456706553796 1103 86-87 4.600849902222905 1103 88-89 4.258727807360611 1103 90-91 3.90897965315186 1103 92-93 3.452519291310633 1103 94-95 3.9109700415368316 1103 96-97 3.7862178164335596 1103 98-99 3.613490743619071 1103 100-101 3.0359788929614275 1104 1 -0.19496012376222538 1104 2 -0.006691972601018392 1104 3 -0.2984334360791472 1104 4 0.1204258217775589 1104 5 -0.14998340080410344 1104 6 0.2171331779511121 1104 7 -0.16156770227259187 1104 8 -0.36293296379951556 1104 9 -0.2895678561501427 1104 10-11 -0.5030837664733241 1104 12-13 -0.6078526301960068 1104 14-15 -0.33169857747113696 1104 16-17 -0.0582120108669244 1104 18-19 -0.5040190507268321 1104 20-21 0.29300497040687645 1104 22-23 -0.2730867369704839 1104 24-25 -0.11483207227392 1104 26-27 -0.2787305420993462 1104 28-29 -0.1946128243160814 1104 30-31 -1.0484146966797923 1104 32-33 -2.039075384187818 1104 34-35 -1.3342736170420295 1104 36-37 -0.8362943555935729 1104 38-39 -0.6520610186744804 1104 40-41 -0.3112714104457055 1104 42-43 0.5556481616943998 1104 44-45 0.7725758707601997 1104 46-47 0.34641774151594973 1104 48-49 0.5209597952463625 1104 50-51 -0.2790984929422038 1104 52-53 -0.3162396504655902 1104 54-55 -0.7858142509233588 1104 56-57 -1.307963409581376 1104 58-59 -0.8740099261872132 1104 60-61 -1.1415159041366714 1104 62-63 -0.6825812529958171 1104 64-65 -2.588929545639779 1104 66-67 -2.9348541966547295 1104 68-69 -2.1947675260283965 1104 70-71 -2.1897060994210094 1104 72-73 -2.0199226138075907 1104 74-75 -1.958065991550086 1104 76-77 -2.1477076762433107 1104 78-79 -1.6038018904466362 1104 80-81 -1.7968730283291912 1104 82-83 -1.952280090637096 1104 84-85 -2.186751027964103 1104 86-87 -2.0684080021408278 1104 88-89 -2.7236996815340895 1104 90-91 -2.149551833652424 1104 92-93 -2.4514524881510873 1104 94-95 -3.9805899271070153 1104 96-97 -3.440134417692388 1104 98-99 -2.240768467251062 1104 100-101 -2.296396335677194 1105 1 -0.0239006922376781 1105 2 -0.05268680464236297 1105 3 0.3553133339466896 1105 4 -0.24804963041881933 1105 5 -0.10398856876275886 1105 6 -0.12472728716516457 1105 7 -0.06260129400386205 1105 8 0.2528293101022925 1105 9 -0.12186759775220679 1105 10-11 0.0404562852062611 1105 12-13 0.28219904939054885 1105 14-15 0.3026683475934604 1105 16-17 0.3610386351279118 1105 18-19 -0.3546650455588676 1105 20-21 -0.32727926731921997 1105 22-23 -0.10254410131157243 1105 24-25 -0.23162793790699965 1105 26-27 0.07398263619523249 1105 28-29 0.04001249868133527 1105 30-31 -0.1822648258787538 1105 32-33 0.24697112744008365 1105 34-35 0.08342664135590638 1105 36-37 0.48670306042709655 1105 38-39 0.10439892964593867 1105 40-41 0.48976218128556326 1105 42-43 0.7696016500664982 1105 44-45 0.4270978345844867 1105 46-47 0.8767794986218931 1105 48-49 1.1613990717321485 1105 50-51 1.1175423339311834 1105 52-53 1.5280755950124458 1105 54-55 1.2371831650973206 1105 56-57 1.6070236705219827 1105 58-59 1.758289815414848 1105 60-61 1.7848406850106109 1105 62-63 1.7534652586320902 1105 64-65 2.6242487489338693 1105 66-67 2.490081203862072 1105 68-69 2.3454133525245773 1105 70-71 1.7030587584601307 1105 72-73 2.0667698926523563 1105 74-75 2.5693241893284693 1105 76-77 1.626710928407853 1105 78-79 1.6938725281580176 1105 80-81 1.0005429923426412 1105 82-83 1.4969447155644566 1105 84-85 1.4144117627335717 1105 86-87 1.7584653828720924 1105 88-89 2.0883158223418796 1105 90-91 1.5479933859858193 1105 92-93 1.5052658581021454 1105 94-95 1.4968002537715392 1105 96-97 2.280537416932937 1105 98-99 2.0078103441184467 1105 100-101 2.1474021139352075 1106 1 0.2738770855400965 1106 2 -0.2415756935312494 1106 3 -0.049131110497754094 1106 4 0.019728147358954118 1106 5 0.08490032012613113 1106 6 -0.224727287165166 1106 7 0.01517648377392078 1106 8 0.18171819899117736 1106 9 0.4836879578033475 1106 10-11 -0.27843260368262435 1106 12-13 -0.16668983949833915 1106 14-15 -0.13344276351765672 1106 16-17 -0.39007247598320305 1106 18-19 -0.10355393444775274 1106 20-21 -0.398390378430328 1106 22-23 0.3191225653550944 1106 24-25 0.3822609509818946 1106 26-27 0.6073159695285639 1106 28-29 0.8894569431257793 1106 30-31 1.0799573963434668 1106 32-33 0.816971127440091 1106 34-35 1.1089821969114624 1106 36-37 0.82114750487154 1106 38-39 0.7082878185348278 1106 40-41 0.7119844035077847 1106 42-43 1.1646016500664942 1106 44-45 1.2643200568067101 1106 46-47 0.9895572763996654 1106 48-49 1.4508435161765902 1106 50-51 1.5103201117089569 1106 52-53 1.6308533727902272 1106 54-55 1.3516276095417652 1106 56-57 1.4942458927442104 1106 58-59 1.6477342598592983 1106 60-61 1.4615073516772803 1106 62-63 1.0184652586320908 1106 64-65 1.1875820822672019 1106 66-67 1.4411923149731756 1106 68-69 1.3143022414134649 1106 70-71 0.9275032029045747 1106 72-73 0.3006587815412445 1106 74-75 0.08821307821735758 1106 76-77 0.21671092840785278 1106 78-79 0.7588725281580153 1106 80-81 0.17498743678708806 1106 82-83 0.4452780488977872 1106 84-85 0.5894117627335724 1106 86-87 1.036243160649871 1106 88-89 1.436649155675216 1106 90-91 0.9002156082080397 1106 92-93 1.6847103025465877 1106 94-95 1.5956891426604294 1106 96-97 1.5799818613773802 1106 98-99 1.3822547885628929 1106 100-101 2.069068780601878 1107 1 0.040475023684429345 1107 2 -0.5054932193044444 1107 3 -0.2643888424565155 1107 4 -0.12388010006372241 1107 5 -0.6150996798738717 1107 6 -0.3948303799486723 1107 7 -0.24152454715391514 1107 8 -0.387147780390265 1107 9 -0.4415697741554183 1107 10-11 0.15053646848232205 1107 12-13 -0.4283393240344253 1107 14-15 -0.2800922480537409 1107 16-17 -0.47429928010691214 1107 18-19 0.10644606555224101 1107 20-21 -0.3038542959560999 1107 22-23 0.056390606592209735 1107 24-25 -0.23500709025521616 1107 26-27 0.4124706087038206 1107 28-29 -0.14234718058555984 1107 30-31 -0.3203518820070528 1107 32-33 0.1624350449658678 1107 34-35 -0.23627553504730514 1107 36-37 0.08836399971689701 1107 38-39 -0.04794929486723731 1107 40-41 -0.02770631814169633 1107 42-43 0.07063257790154154 1107 44-45 0.02767057227062253 1107 46-47 0.6236294413481289 1107 48-49 0.7826476398879336 1107 50-51 1.2200623797501962 1107 52-53 0.9321420325840393 1107 54-55 0.7728647229438224 1107 56-57 1.1503799133627624 1107 58-59 0.7831466309933219 1107 60-61 0.8587238465226399 1107 62-63 0.5318157740960068 1107 64-65 0.43180888639090753 1107 66-67 0.4625325211587459 1107 68-69 0.19512698368150438 1107 70-71 0.8485341307396261 1107 72-73 0.8520505341185647 1107 74-75 0.23037802667096585 1107 76-77 0.2122779387171292 1107 78-79 0.42376943537450984 1107 80-81 0.34086372544688004 1107 82-83 0.4374945437431492 1107 84-85 0.0675045462387267 1107 86-87 0.5903668719900779 1107 88-89 1.4216491556752153 1107 90-91 1.028463030888453 1107 92-93 1.184246385020817 1107 94-95 1.2284726478150674 1107 96-97 0.5894148510681028 1107 98-99 0.6228733452639226 1107 100-101 1.0743780589523908 1108 1 0.03808761185588949 1108 2 -0.23104937774177614 1108 3 -0.2575521631293327 1108 4 0.013938673674743995 1108 5 -0.17299441671598004 1108 6 -0.30367465558622087 1108 7 -0.40061298991029304 1108 8 -0.33722916942987524 1108 9 -0.18210151588086632 1108 10-11 -0.501064182629996 1108 12-13 0.11962594997534381 1108 14-15 0.0678730259560254 1108 16-17 -0.031651423351618746 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-1.9161775985107674 2316 64-65 -1.8325964891613715 2316 66-67 -2.155057685026822 2316 68-69 -1.7549834728722509 2316 70-71 -1.5444610828097112 2316 72-73 -1.6496983613158989 2316 74-75 -1.4712512074969268 2316 76-77 -1.794717643020718 2316 78-79 -1.2855917575562685 2316 80-81 -1.3103697060700554 2316 82-83 -2.3849005225307813 2316 84-85 -2.307909665837858 2316 86-87 -2.0307211250644137 2316 88-89 -3.0828151300390694 2316 90-91 -2.9860343917919607 2316 92-93 -3.027968268881981 2316 94-95 -2.935739428768141 2316 96-97 -3.1937681386226195 2316 98-99 -3.165602354294254 2316 100-101 -2.806466933683838 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 11.0 5 33.0 6 97.0 7 118.0 8 102.0 9 158.0 10 294.0 11 375.0 12 475.0 13 691.0 14 834.0 15 1065.0 16 1295.0 17 1592.0 18 1468.0 19 1111.0 20 1052.0 21 1220.0 22 1390.0 23 1389.0 24 1521.0 25 1730.0 26 1739.0 27 1873.0 28 2143.0 29 2581.0 30 2904.0 31 3587.0 32 4139.0 33 5372.0 34 8104.0 35 13159.0 36 11882.0 37 15451.0 38 5453.0 39 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.20631302881763 21.75632499713801 18.949493688011906 11.08786828603245 2 14.369121322423753 31.390971595681798 30.917047776083955 23.322859305810493 3 29.529498387414577 27.253212207945744 34.704290203154656 8.51299920148503 4 10.021881384230886 22.39886341245891 41.278038764272154 26.30121643903805 5 6.875524997666677 44.488691161372614 35.604434350662146 13.03134949029856 6 19.93591139594935 39.91226705659086 15.446598015119934 24.705223532339858 7 15.553412355204346 41.11315060821952 24.140040859077665 19.19339617749847 8 21.157535596138093 24.851445104688423 21.557830113347645 32.43318918582584 9 14.836822947453568 28.660465212747205 29.857200634663844 26.645511205135385 10-11 23.694116915035934 26.37640129006834 25.31966524593224 24.609816548963487 12-13 32.16978294911282 27.20187910275954 24.979000093332917 15.64933785479472 14-15 21.700353635392577 32.034596119344165 34.14448235452726 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15.203729245958083 34.46648760202018 33.197652109886235 17.1321310421355 42-43 14.512307046153367 34.223284609840455 28.832243579439687 22.432164764566494 44-45 17.792365367265035 27.79195055429383 31.701044291655002 22.714639786786133 46-47 21.940204090098312 32.98263989712532 32.48226656157963 12.594889451196748 48-49 18.654139314936376 30.16519926578104 33.95866388741976 17.22199753186282 50-51 9.958207159746133 32.73271249014809 40.21964574604887 17.089434604056912 52-53 16.200002074064855 36.01095106243972 29.008389592342553 18.78065727115287 54-55 13.09823808190482 29.18572213753124 32.89155751900362 24.82448226156032 56-57 19.665245932240303 27.568470066058964 30.939862489500047 21.826421512200685 58-59 24.64974229744164 28.037208723516784 30.284976511215504 17.028072467826068 60-61 17.016411293458816 32.2330248113868 36.12662362915143 14.623940266002954 62-63 13.090978854908794 32.63541050928663 34.87903016727333 19.39458046853125 64-65 12.463574235966359 38.80782752076657 30.306754192203588 18.421844051063477 66-67 16.89913718007218 30.903368316256692 27.20008711162733 24.997407392043804 68-69 13.794229891732693 31.117517733438422 31.516260837101257 23.571991537727634 70-71 20.946499497039273 31.34586068506362 27.693950989847455 20.013688828049652 72-73 17.682439929896606 26.42306774932852 36.904873015379195 18.98961930539568 74-75 13.232015265117338 37.521907310041584 29.46157276338031 19.784504661460765 76-77 15.91534380547971 29.489508819651366 29.3291043304021 25.266043044466823 78-79 16.61118543176845 30.43379066463408 32.277115805411235 20.67790809818623 80-81 20.1811695651723 28.491428926982547 29.941200261332174 21.38620124651298 82-83 16.223335303694945 27.551359030996895 35.55880492382997 20.666500741478185 84-85 15.6441526926547 30.981862302834212 32.299930518827324 21.074054485683767 86-87 15.397716356763436 32.27268714918453 30.28189857051618 22.047697923535857 88-89 14.467209913826768 31.620629507172428 27.11098383275624 26.801176746244565 90-91 17.21174604000312 26.849429084193826 29.192394725206128 26.74643015059693 92-93 19.867985771915087 31.061713799790517 30.16364371713903 18.90665671115536 94-95 17.517551773843966 28.808242333737777 31.10682471040869 22.567381182009562 96-97 11.375326668602481 30.674700294520264 35.35176504749658 22.59820798938068 98-99 16.33014964377936 32.48192970994203 27.357433966960148 23.83048667931846 100-101 14.553194578394466 28.69365025044333 29.94068174511817 26.812473426044033 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7814.0 1 4492.0 2 995.5 3 724.5 4 519.5 5 502.0 6 857.5 7 1604.0 8 1844.0 9 1394.5 10 856.5 11 435.0 12 317.0 13 277.0 14 235.5 15 222.5 16 238.5 17 234.0 18 223.0 19 220.5 20 226.5 21 239.5 22 254.5 23 274.0 24 295.5 25 336.0 26 386.5 27 442.5 28 536.0 29 600.5 30 643.5 31 698.0 32 748.0 33 770.0 34 731.5 35 647.5 36 610.0 37 554.5 38 410.0 39 307.0 40 280.5 41 282.0 42 297.0 43 319.0 44 395.0 45 684.5 46 2041.0 47 7965.5 48 17612.0 49 17758.5 50 9946.5 51 5050.5 52 2131.0 53 669.0 54 241.0 55 133.5 56 104.5 57 92.5 58 88.0 59 75.5 60 64.5 61 56.5 62 43.0 63 38.0 64 38.0 65 29.0 66 25.5 67 21.5 68 20.0 69 22.0 70 19.5 71 16.0 72 11.5 73 8.5 74 6.5 75 6.0 76 6.5 77 7.0 78 4.5 79 2.5 80 1.5 81 1.5 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3546650903773761 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.002074064856008047 16-17 0.0036296134980140825 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.003111097284012071 36-37 0.0067407107820261535 38-39 0.015036970206058344 40-41 0.002074064856008047 42-43 5.185162140020118E-4 44-45 0.0 46-47 0.0010370324280040235 48-49 0.0 50-51 0.0010370324280040235 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0015555486420060355 62-63 0.0 64-65 0.0 66-67 0.0010370324280040235 68-69 0.0010370324280040235 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.1140735670804426 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14051789399454523 88-89 0.4163685198436155 90-91 0.3230356013232534 92-93 0.0 94-95 0.0 96-97 0.0010370324280040235 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 96429.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.289404639683084 #Duplication Level Percentage of deduplicated Percentage of total 1 81.33397312859884 14.06215972373456 2 8.523272552783109 2.947246160387435 3 3.2449616122840688 1.6831036306505305 4 1.6854606525911708 1.1656244490765226 5 0.9956813819577736 0.8607369152433396 6 0.6837811900191938 0.7093301807547522 7 0.4678502879078695 0.5662197056901969 8 0.31190019193857965 0.4314054900496738 9 0.2579174664107486 0.4013315496375572 >10 1.859404990403071 6.373601302512729 >50 0.19193857965451055 2.1715459042404257 >100 0.26991362763915544 10.27076916695185 >500 0.04798464491362764 6.284416513704384 >1k 0.10796545105566219 33.178815501560734 >5k 0.017994241842610365 18.89369380580531 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7099 7.361893206400564 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5842 6.058343444399506 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5278 5.473457155005237 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4231 4.3876842028850245 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2817 2.921320349687335 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2098 2.1756940339524418 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2086 2.1632496448163936 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2023 2.0979166018521402 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1986 2.059546402015991 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1964 2.0367316885999025 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1769 1.834510365139118 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1674 1.7359922844787359 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1541 1.5980669715542004 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1391 1.442512107353597 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1336 1.3854753238133757 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1257 1.3035497620010577 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1212 1.2568833027408768 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1174 1.2174760704767238 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1172 1.2154020056207158 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1154 1.1967354219166433 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1109 1.1500689626564622 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 991 1.0276991361519874 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 926 0.960292028331726 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 834 0.8648850449553557 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 711 0.7373300563108609 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 690 0.7155523753227764 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 678 0.7031079861867281 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 630 0.6533304296425348 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 600 0.6222194568024142 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 491 0.5091829221499756 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 443 0.4594053656057825 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 436 0.4521461386097543 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 435 0.4511091061817503 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 384 0.3982204523535451 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 379 0.393035290213525 No Hit ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT 351 0.36399838222941233 RNA PCR Primer, Index 33 (95% over 22bp) AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 345 0.3577761876613882 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 309 0.32044302025324334 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 290 0.30073940412116684 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 284 0.2945172095531427 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 278 0.28829501498511856 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 252 0.26133217185701396 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 249 0.2582210745730019 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 239 0.2478507502929617 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 226 0.23436932872890937 No Hit GTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 220 0.2281471341608852 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 220 0.2281471341608852 No Hit TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 203 0.2105175828848168 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 196 0.20325835588878866 No Hit ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT 195 0.20222132346078464 RNA PCR Primer, Index 33 (95% over 24bp) TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG 194 0.2011842910327806 RNA PCR Primer, Index 33 (96% over 29bp) GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 192 0.19911022617677254 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 179 0.18562880461272024 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 172 0.1783695776166921 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 171 0.17733254518868805 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 167 0.17318441547667196 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 162 0.16799925333665183 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 160 0.16592518848064378 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 155 0.16074002634062368 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 152 0.15762892905661158 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 142 0.14725860477657135 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 142 0.14725860477657135 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 138 0.14311047506455526 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 136 0.1410364102085472 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 134 0.13896234535253918 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 131 0.1358512480685271 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 130 0.1348142156405231 No Hit TGATACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 129 0.13377718321251905 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 124 0.12859202107249895 No Hit ACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 121 0.12548092378848685 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 119 0.12340685893247882 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 117 0.12133279407647077 No Hit TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 111 0.11511059950844664 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 101 0.10474027522840638 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0010370324280040237 0.0 12-13 0.0 0.0 0.0 0.0010370324280040237 0.0 14-15 0.0 0.0 0.0 0.0015555486420060355 0.0 16-17 0.0 0.0 0.0 0.003111097284012071 0.0 18-19 0.0 0.0 0.0 0.005185162140020119 0.0 20-21 0.0 0.0 0.0 0.008814775638034202 0.0 22-23 0.0 0.0 0.0 0.01711103506206639 0.0 24-25 0.0 0.0 0.0 0.03007394041211669 0.0 26-27 0.0 0.0 0.0 0.06377749432224745 0.0 28-29 0.0 0.0 0.0 0.17162886683466594 0.0 30-31 0.0 0.0 0.0 0.4122203901315994 0.0 32-33 0.0 0.0 0.0 0.7819224507150339 0.0 34-35 0.0 0.0 0.0 1.2309574920407762 0.0 36-37 0.0 0.0 0.0 1.7240664115566893 0.0 38-39 0.0 0.0 0.0 2.4079892978253428 0.0 40-41 0.0 0.0 0.0 3.0711715355339164 0.0 42-43 0.0 0.0 0.0 3.7421315164525195 0.0 44-45 0.0 0.0 0.0 4.46131350527331 0.0 46-47 0.0 0.0 0.0 5.196569496728163 0.0 48-49 0.0 0.0 0.0 5.739974489002272 0.0 50-51 0.0 0.0 0.0 6.3030830974084555 0.0 52-53 0.0 0.0 0.0 6.8013771790643895 0.0 54-55 0.0 0.0 0.0 7.335967395700464 0.0 56-57 0.0 0.0 0.0 8.122037976127514 0.0 58-59 0.0 0.0 0.0 8.634850511775504 0.0 60-61 0.0 0.0 0.0 9.219736801169773 0.0 62-63 0.0 0.0 0.0 9.80410457435004 0.0 64-65 0.0 0.0 0.0 10.407657447448383 0.0 66-67 0.0 0.0 0.0 11.111802466063114 0.0 68-69 0.0 0.0 0.0 11.969428284022442 0.0 70-71 0.0 0.0 0.0 12.814091196631718 0.0 72-73 0.0 0.0 0.0 13.480903047838307 0.0 74-75 0.0 0.0 0.0 14.413195200613924 0.0 76-77 0.0 0.0 0.0 15.044229433054372 0.0 78-79 0.0 0.0 0.0 16.277260989951156 0.0 80-81 0.0 0.0 0.0 17.338145163799272 0.0 82-83 0.0 0.0 0.0 18.078586317394148 0.0 84-85 0.0 0.0 0.0 19.03835982951187 0.0 86-87 0.0010370324280040237 0.0 0.0 19.841541445000985 0.0 88-89 0.0010370324280040237 0.0 0.0 20.854203610946914 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 20 5.397561E-4 47.463173 94-95 GTACCCA 45 9.495574E-4 42.299187 1 CGCAGAA 345 0.0 38.52084 9 CTTTGAT 25 0.0016186369 37.970543 22-23 GCTTTGC 200 0.0 35.68994 1 CTTTGCG 210 0.0 33.902267 2 GTTGATC 50 1.2996225E-6 33.224224 94-95 TTTGCGT 215 0.0 33.113842 3 TATCTCT 30 0.0038435785 31.831987 84-85 CCACTGT 30 0.0039578695 31.642117 94-95 CATGGGG 30 0.0039578695 31.642117 16-17 AAGCAGG 30 0.0039578695 31.642117 94-95 TTGCGTT 235 0.0 30.295645 4 CAGAAAA 290 0.0 29.459902 10-11 ATAAGCA 65 0.0058399905 29.208105 5 GGGGGTT 50 0.0016561347 23.731588 54-55 GAGTACT 810 0.0 21.973692 12-13 CACCCAC 55 0.0027833332 21.703625 84-85 GCTTCCA 110 0.0028713872 21.574171 9 GTACTTT 850 0.0 20.66044 14-15 >>END_MODULE