##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939055_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 972976 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.796717493545575 33.0 31.0 34.0 30.0 34.0 2 31.78461030899015 33.0 31.0 34.0 30.0 34.0 3 31.85202821035668 33.0 31.0 34.0 30.0 34.0 4 35.32447665718373 37.0 35.0 37.0 33.0 37.0 5 35.411927940668626 37.0 35.0 37.0 33.0 37.0 6 35.566641931558436 37.0 35.0 37.0 33.0 37.0 7 35.52124512834849 37.0 35.0 37.0 33.0 37.0 8 35.546422522241045 37.0 35.0 37.0 33.0 37.0 9 37.07621873509727 39.0 37.0 39.0 33.0 39.0 10-11 36.9785888860568 39.0 37.0 39.0 33.0 39.0 12-13 36.91396601766128 39.0 37.0 39.0 33.0 39.0 14-15 37.91193564897798 40.0 38.0 41.0 33.0 41.0 16-17 37.9087017562612 40.0 37.5 41.0 33.0 41.0 18-19 37.91484116771637 40.0 38.0 41.0 33.0 41.0 20-21 38.02176723783526 40.0 38.0 41.0 33.0 41.0 22-23 37.98864360477545 40.0 38.0 41.0 33.0 41.0 24-25 37.85453443867064 40.0 37.5 41.0 32.5 41.0 26-27 37.62779606074559 40.0 37.0 41.0 32.5 41.0 28-29 37.529009451415035 40.0 37.0 41.0 32.0 41.0 30-31 37.432422279686236 40.0 37.0 41.0 32.0 41.0 32-33 37.2047881962145 40.0 36.0 41.0 31.5 41.0 34-35 36.82186816529904 39.0 35.0 41.0 30.5 41.0 36-37 36.48152677969446 39.0 35.0 41.0 30.0 41.0 38-39 36.18175422620908 39.0 35.0 40.5 30.0 41.0 40-41 36.11639290177764 39.0 35.0 40.0 30.0 41.0 42-43 35.95104349953134 39.0 35.0 40.0 30.0 41.0 44-45 35.89120851901794 39.0 35.0 41.0 29.5 41.0 46-47 35.62295575635987 39.0 35.0 41.0 27.5 41.0 48-49 35.32090000164444 39.0 35.0 41.0 26.0 41.0 50-51 34.11232496998898 38.0 34.0 39.5 24.0 40.5 52-53 34.268624303168835 38.0 33.5 39.5 24.0 40.5 54-55 34.83735518656164 38.0 35.0 40.0 24.5 41.0 56-57 34.761629269375604 38.5 34.5 40.0 24.5 41.0 58-59 34.602246612454984 38.0 34.0 40.0 24.0 41.0 60-61 34.12573948381049 38.0 34.0 40.0 22.0 41.0 62-63 33.781927303448384 37.5 33.5 40.0 22.0 41.0 64-65 33.31923706237359 37.0 33.0 39.5 21.5 41.0 66-67 32.76658160119057 36.0 32.0 39.0 19.5 40.5 68-69 32.3050871758399 36.0 32.0 39.0 18.0 40.0 70-71 31.650709267237836 35.0 31.5 37.5 13.0 39.0 72-73 31.103462983670717 35.0 31.0 37.0 8.5 39.0 74-75 30.657872856062227 35.0 31.0 36.5 6.0 39.0 76-77 30.192645039548765 34.5 30.0 36.0 2.0 37.5 78-79 29.74366788081104 34.0 30.0 35.5 2.0 37.0 80-81 29.251402912302048 34.0 29.0 35.0 2.0 36.5 82-83 28.737330108861883 34.0 29.0 35.0 2.0 36.0 84-85 28.276999638223348 34.0 29.0 35.0 2.0 36.0 86-87 27.887147781651347 34.0 27.5 35.0 2.0 35.0 88-89 27.269031301902615 33.5 25.5 35.0 2.0 35.0 90-91 26.680872395619215 33.0 25.0 35.0 2.0 35.0 92-93 26.144262551183175 33.0 24.5 35.0 2.0 35.0 94-95 25.529199075825098 33.0 20.5 35.0 2.0 35.0 96-97 24.7246221900643 32.0 12.5 35.0 2.0 35.0 98-99 23.884476595517256 32.0 2.0 34.0 2.0 35.0 100-101 20.8620741929914 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.006811491397268554 1101 2 0.18501594759513296 1101 3 0.11134133721605366 1101 4 0.0795715744548744 1101 5 -0.012833012422611034 1101 6 0.2430217062092339 1101 7 0.21715173742004623 1101 8 0.07536383000340408 1101 9 0.16274775029489774 1101 10-11 0.01953181962822015 1101 12-13 0.1111687864751616 1101 14-15 0.23596553321397096 1101 16-17 0.3425279135050303 1101 18-19 0.4338114566670157 1101 20-21 0.11800002310425839 1101 22-23 0.17444369744521993 1101 24-25 0.2100855007838902 1101 26-27 0.19924093634511308 1101 28-29 0.2303095649654594 1101 30-31 0.1254896064518931 1101 32-33 0.06413485974864841 1101 34-35 -0.08091953343409841 1101 36-37 -0.14621965870806974 1101 38-39 0.09668393946351728 1101 40-41 0.04345576218813818 1101 42-43 -0.0974342392143086 1101 44-45 0.04856237822623655 1101 46-47 -0.014114877698695238 1101 48-49 -0.28561133752914003 1101 50-51 -0.22572262532345633 1101 52-53 0.044702556266393856 1101 54-55 0.15321230667253616 1101 56-57 0.15907175893277525 1101 58-59 0.22806919002340464 1101 60-61 0.1692925404360892 1101 62-63 0.030700291411385194 1101 64-65 0.012240908172714171 1101 66-67 -0.12193452946349481 1101 68-69 -0.12382502790590166 1101 70-71 -0.26096362439059817 1101 72-73 -0.4424895113435561 1101 74-75 -0.38365311575430283 1101 76-77 -0.4142592835645509 1101 78-79 -0.5387846930593696 1101 80-81 -0.33403120760961613 1101 82-83 -0.3188974364041286 1101 84-85 -0.37312148123368516 1101 86-87 -0.4845553644774263 1101 88-89 -0.5701948181246799 1101 90-91 -0.7043631987034971 1101 92-93 -0.47621622656184215 1101 94-95 -0.5178756903022297 1101 96-97 -0.6831740240962318 1101 98-99 -0.6587372689656341 1101 100-101 -0.25062012758116126 1102 1 0.38735839504862923 1102 2 0.5194572411968537 1102 3 0.5001734076296387 1102 4 0.3927963328472188 1102 5 0.3208154051828487 1102 6 0.6473936296179303 1102 7 0.5197927634584047 1102 8 0.5803612731720165 1102 9 0.6659864033871088 1102 10-11 0.6486001515927526 1102 12-13 0.5723023667105949 1102 14-15 0.7900011945551952 1102 16-17 0.9068645061527221 1102 18-19 0.9308878878469216 1102 20-21 0.8443919466162626 1102 22-23 0.8537163176550351 1102 24-25 0.8415983185153877 1102 26-27 0.9208768952085364 1102 28-29 0.9057985912517594 1102 30-31 0.9027818445316314 1102 32-33 0.6443651295330994 1102 34-35 0.8168640294975233 1102 36-37 0.8383722038532042 1102 38-39 0.7509938790809798 1102 40-41 0.5270881673141901 1102 42-43 0.7912105884965754 1102 44-45 0.9492837145967741 1102 46-47 1.2168431851012187 1102 48-49 1.2637949094039556 1102 50-51 1.0947297013931063 1102 52-53 1.198174423373139 1102 54-55 1.3388305175104023 1102 56-57 1.2497850374104544 1102 58-59 1.2971410860559232 1102 60-61 1.4590254419490094 1102 62-63 1.5482494523058037 1102 64-65 1.3001155872774603 1102 66-67 1.184845205969033 1102 68-69 1.346626782279074 1102 70-71 1.1933220382419627 1102 72-73 1.1165681284169793 1102 74-75 1.0054152678633805 1102 76-77 1.1199751391339134 1102 78-79 1.0247254490384705 1102 80-81 0.9535464658287793 1102 82-83 1.0179147372124682 1102 84-85 0.8203802930523416 1102 86-87 0.8178080258293399 1102 88-89 0.4581472704191647 1102 90-91 0.7224094085930233 1102 92-93 0.5693883478419544 1102 94-95 0.1949921447589027 1102 96-97 0.432646563820029 1102 98-99 0.33962661721146503 1102 100-101 0.2914500793930479 1103 1 0.07456258321159126 1103 2 0.08200575053347237 1103 3 0.13125134215915324 1103 4 0.17695071048193967 1103 5 -0.09548221006011204 1103 6 0.051326119839970374 1103 7 -0.03148636116683434 1103 8 -0.008941562056037355 1103 9 -0.011272419605170114 1103 10-11 0.21008335785064247 1103 12-13 0.18429996216936217 1103 14-15 0.3188937359117858 1103 16-17 0.18478686077690298 1103 18-19 0.4043770598020586 1103 20-21 0.33523794944692753 1103 22-23 0.2364949324566652 1103 24-25 0.22460140789431904 1103 26-27 0.12645756147951914 1103 28-29 0.26904030852031724 1103 30-31 0.3193229576211678 1103 32-33 0.21128518614635539 1103 34-35 0.03359994086430618 1103 36-37 0.21411601368138378 1103 38-39 0.3387482201910288 1103 40-41 0.08233174125383869 1103 42-43 0.2265466916179193 1103 44-45 0.33351190251624274 1103 46-47 0.3106279025676244 1103 48-49 0.01842136848870979 1103 50-51 -0.02543648588717673 1103 52-53 -0.07168586973739366 1103 54-55 -0.02759189037892895 1103 56-57 -0.06963467678394863 1103 58-59 -0.025638137490318513 1103 60-61 0.015128867659342404 1103 62-63 -0.05328914971217813 1103 64-65 -0.23985991075173274 1103 66-67 -0.3195643878461283 1103 68-69 -0.08103533402159258 1103 70-71 -0.2249235597735364 1103 72-73 -0.30059314325052355 1103 74-75 -0.3907747517686353 1103 76-77 -0.2836339555106555 1103 78-79 -0.35582744325938975 1103 80-81 -0.4206374359198364 1103 82-83 -0.28615319868672273 1103 84-85 -0.05021962476713426 1103 86-87 -0.21359558706995685 1103 88-89 -0.2872072472268208 1103 90-91 -0.09513080659819906 1103 92-93 -0.20508318573792295 1103 94-95 -0.3293994910900686 1103 96-97 -0.40619971294571044 1103 98-99 -0.0695562953271498 1103 100-101 0.16707974960565153 1104 1 0.08931264023188135 1104 2 0.2628352865581647 1104 3 0.352608958856635 1104 4 0.3983422967136008 1104 5 0.2811550001676295 1104 6 0.24217181565735046 1104 7 0.17943290295600178 1104 8 0.35571653458243446 1104 9 0.25864949899411727 1104 10-11 0.30719012409657154 1104 12-13 0.22333580973349854 1104 14-15 0.4672605292362917 1104 16-17 0.5016316248584829 1104 18-19 0.5559758116643181 1104 20-21 0.5376903071752324 1104 22-23 0.5196746749675967 1104 24-25 0.4236835025528549 1104 26-27 0.5537008852528587 1104 28-29 0.6723860649975819 1104 30-31 0.6404454566142093 1104 32-33 0.48538657064750623 1104 34-35 0.5414189997140966 1104 36-37 0.8809064265082824 1104 38-39 0.6645961240281224 1104 40-41 0.6380344981241848 1104 42-43 0.6429476679993869 1104 44-45 0.9268686748281567 1104 46-47 1.1098060824799845 1104 48-49 1.2250604713047792 1104 50-51 0.9581477367200364 1104 52-53 1.1515182337823333 1104 54-55 1.0332208549902973 1104 56-57 0.9520221144538539 1104 58-59 0.8887367988168009 1104 60-61 1.134573029024338 1104 62-63 1.0873835441204065 1104 64-65 1.0496163275591073 1104 66-67 0.8139407342743468 1104 68-69 1.0196928272075212 1104 70-71 0.9976909296886269 1104 72-73 0.9891560645314144 1104 74-75 1.0146564914677931 1104 76-77 1.1981510949826308 1104 78-79 0.8193231145688884 1104 80-81 0.7164878679861744 1104 82-83 0.8944992104811682 1104 84-85 1.0204297271426803 1104 86-87 1.2389040853666806 1104 88-89 0.8023376580709716 1104 90-91 0.8135845626749045 1104 92-93 0.7601781127717864 1104 94-95 1.0040806391588326 1104 96-97 0.8381831819379926 1104 98-99 0.8853260972927224 1104 100-101 1.281554653399077 1105 1 0.16851857372433443 1105 2 0.11243155466805987 1105 3 0.16347284111540716 1105 4 0.15845131278791058 1105 5 0.2143911186343388 1105 6 0.03802301403613484 1105 7 0.28450355301391994 1105 8 0.25004252359860857 1105 9 0.15111824876740343 1105 10-11 0.19314584054837525 1105 12-13 0.2941890031935017 1105 14-15 0.10107969507469505 1105 16-17 0.2784678855200795 1105 18-19 0.20137188969738418 1105 20-21 0.3137569501427535 1105 22-23 -0.059878886312837665 1105 24-25 0.3411868941909191 1105 26-27 0.1594347486732275 1105 28-29 0.024859482051773796 1105 30-31 0.13827202722679033 1105 32-33 0.012434430832215071 1105 34-35 0.10081317214231689 1105 36-37 0.16531613638719733 1105 38-39 0.3785752349232041 1105 40-41 0.29936764766821256 1105 42-43 0.3199041171901058 1105 44-45 0.31522574358228184 1105 46-47 0.1801145583104784 1105 48-49 0.33979275558814237 1105 50-51 0.27278353162085267 1105 52-53 0.27181558552361906 1105 54-55 0.5212697139247524 1105 56-57 0.4272359508862138 1105 58-59 0.42260844133694064 1105 60-61 0.6827663318389767 1105 62-63 0.5237573812298919 1105 64-65 0.4787135593536931 1105 66-67 0.5738611244880545 1105 68-69 0.47701985366592936 1105 70-71 0.13798262265409633 1105 72-73 0.004747926534669489 1105 74-75 0.18505033259161152 1105 76-77 -0.01704622459552496 1105 78-79 -0.06796446973840631 1105 80-81 0.14331735746302598 1105 82-83 0.019814493770610397 1105 84-85 0.06032409666073946 1105 86-87 0.021995832501620072 1105 88-89 -0.33024032542184756 1105 90-91 -0.14350607688767525 1105 92-93 0.14731818198296764 1105 94-95 0.14467703543757793 1105 96-97 0.06805825357954731 1105 98-99 -0.14812355532131605 1105 100-101 0.1809608446802109 1106 1 0.26751024279518276 1106 2 0.23256932309030987 1106 3 0.2873112659678583 1106 4 0.10995454863619614 1106 5 0.23395936351165858 1106 6 0.10458140355604684 1106 7 0.12042449644979314 1106 8 0.3197388133254577 1106 9 0.005731247011460994 1106 10-11 0.14629816583571653 1106 12-13 0.17211364372406024 1106 14-15 0.3208235341406578 1106 16-17 0.33332593204362837 1106 18-19 0.2670665552670357 1106 20-21 0.2505448231023166 1106 22-23 0.22136209801191598 1106 24-25 0.48834764657461704 1106 26-27 0.6192243918216676 1106 28-29 0.4842128110397752 1106 30-31 0.47263470580394085 1106 32-33 0.45671978385489354 1106 34-35 0.44984491088095524 1106 36-37 0.5574574950516862 1106 38-39 0.7225314824077245 1106 40-41 0.8945981105886318 1106 42-43 0.8933455852002581 1106 44-45 0.8856521917000819 1106 46-47 0.9335703445485919 1106 48-49 0.7708729269794645 1106 50-51 0.8106128597682769 1106 52-53 0.8130980285979774 1106 54-55 0.644860597273933 1106 56-57 0.6246084492032864 1106 58-59 0.8802806251516415 1106 60-61 1.0971971110426395 1106 62-63 0.9889484155971999 1106 64-65 0.892627885061323 1106 66-67 0.6874007542799419 1106 68-69 0.6581899592005342 1106 70-71 0.6951005984413321 1106 72-73 0.5895709610729405 1106 74-75 0.6760266434040112 1106 76-77 0.6231156924798533 1106 78-79 0.712539232698898 1106 80-81 0.6358180965257887 1106 82-83 0.7668769419026553 1106 84-85 0.9570921663000433 1106 86-87 1.1473016976324608 1106 88-89 1.3171483788497582 1106 90-91 1.210631427965204 1106 92-93 1.4462058480049578 1106 94-95 1.1761379577166728 1106 96-97 1.3261122568064856 1106 98-99 1.3999618269076954 1106 100-101 1.201446239731517 1107 1 0.021722164836809554 1107 2 0.08254699322669623 1107 3 -0.014610653757664238 1107 4 0.09828428591953298 1107 5 0.19452028082023531 1107 6 0.1427228053395524 1107 7 0.05303596014741885 1107 8 0.0027422963409051704 1107 9 0.09331196420456678 1107 10-11 0.21375042259257526 1107 12-13 0.38503581943959375 1107 14-15 0.3957560181634463 1107 16-17 0.4064781219771305 1107 18-19 0.4486338346474241 1107 20-21 0.30992175895742946 1107 22-23 0.35207934652944317 1107 24-25 0.21893756500136163 1107 26-27 0.05514156187992114 1107 28-29 0.19358991312216034 1107 30-31 0.25620125600069343 1107 32-33 0.245023811320074 1107 34-35 0.21672445927470108 1107 36-37 0.22787812762403092 1107 38-39 0.396869537703779 1107 40-41 0.14186727354565676 1107 42-43 0.2963309161975545 1107 44-45 -0.14896463781391134 1107 46-47 0.07433880214789923 1107 48-49 0.29056808832519465 1107 50-51 0.15969179445093573 1107 52-53 0.05512438337795089 1107 54-55 0.13717868091728747 1107 56-57 0.10984081830761738 1107 58-59 0.3365440133732278 1107 60-61 0.3553452948988678 1107 62-63 0.24862533627021577 1107 64-65 0.2827023437976237 1107 66-67 0.3431063945929296 1107 68-69 0.32984178177175494 1107 70-71 0.2476045906663238 1107 72-73 0.20953275096388424 1107 74-75 0.3818619963987082 1107 76-77 0.313020931852666 1107 78-79 0.5107271337914554 1107 80-81 0.5655294484078652 1107 82-83 0.4484469917375904 1107 84-85 0.6528907165765894 1107 86-87 0.9138094510265447 1107 88-89 1.0720556849816596 1107 90-91 1.0679613303676305 1107 92-93 1.0314654783819925 1107 94-95 1.2238523881278418 1107 96-97 1.676155309670083 1107 98-99 1.5643966153945748 1107 100-101 1.803826171002079 1108 1 0.21849070304428864 1108 2 0.3010603172098669 1108 3 0.23903025471629746 1108 4 0.14671775648913865 1108 5 0.2185995913210732 1108 6 0.29507429608741376 1108 7 0.23428058757355785 1108 8 0.23599570085612953 1108 9 0.49969684408512904 1108 10-11 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-1.5694215750466647 2316 74-75 -1.896756931488465 2316 76-77 -1.7912128553771929 2316 78-79 -1.709261879798646 2316 80-81 -1.7217593646579203 2316 82-83 -1.5400105788366965 2316 84-85 -1.411666946444317 2316 86-87 -1.4049514069852727 2316 88-89 -1.8246269551508938 2316 90-91 -1.9152736760857678 2316 92-93 -2.104313149924536 2316 94-95 -1.9479000335716599 2316 96-97 -1.956858852080238 2316 98-99 -1.654157019276031 2316 100-101 -1.5916287601542152 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3325.0 3 730.0 4 669.0 5 694.0 6 823.0 7 1047.0 8 1280.0 9 1666.0 10 2079.0 11 3002.0 12 4400.0 13 6306.0 14 5516.0 15 5968.0 16 7670.0 17 9888.0 18 8726.0 19 6504.0 20 6615.0 21 7317.0 22 8244.0 23 9080.0 24 10124.0 25 11894.0 26 13949.0 27 16127.0 28 19041.0 29 22350.0 30 26717.0 31 32450.0 32 39291.0 33 49964.0 34 65950.0 35 96796.0 36 135093.0 37 185410.0 38 131845.0 39 14425.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.59105507070061 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18.33051719864702 32.26652735112966 30.19767105832844 19.20528439189488 20-21 21.928674344916526 26.658624352989698 26.993686475234917 24.41901482685886 22-23 19.578547627851815 26.991147484158507 28.43728640791928 24.993018480070397 24-25 24.1809353831594 26.601379466421154 28.088857799745913 21.128827350673532 26-27 23.72425533665752 22.86766426567438 33.372901274728896 20.035179122939205 28-29 19.505944516474774 29.86494013311068 30.848124122800023 19.780991227614525 30-31 20.220930830547697 27.58544613011057 29.08434044741579 23.109282591925943 32-33 20.64185862346471 28.06673643018206 29.670508802287284 21.620896144065945 34-35 23.839323362609065 25.293745289817345 28.962661847288533 21.90426950028506 36-37 22.740095125625 24.52358441121204 32.55741901903183 20.17890144413112 38-39 22.61684375506563 25.81181720471185 31.92758769153889 19.643751348683633 40-41 20.64457587865155 27.721944907400477 31.145915540646907 20.48756367330106 42-43 19.039419854075497 29.47304237923436 27.723228760302586 23.764309006387553 44-45 21.101602411517288 26.516828057536866 27.865406520142965 24.51616301080288 46-47 23.00011875319472 28.377934623784718 27.364195395473956 21.257751227546613 48-49 22.630455349175396 26.80562555772218 28.65029166804376 21.913627425058664 50-51 19.503676109881553 28.329747934221437 32.07737645864467 20.08919949725234 52-53 21.116861308139416 29.944819452304923 27.80653450049366 21.131784739062002 54-55 17.978347850197732 26.60068889918124 30.49517403972607 24.925789210894955 56-57 22.12217653572452 26.98678188956257 27.795820072633788 23.09522150207912 58-59 24.589206936473467 25.861626676012474 27.346288423055302 22.202877964458754 60-61 20.975116708149137 26.34663249006278 31.83815850844715 20.840092293340927 62-63 21.512560528390452 26.191942696090305 30.437229367943946 21.858267407575298 64-65 20.14382284743417 29.80830505936243 29.29906373133789 20.748808361865514 66-67 20.64699857919992 26.560181548398358 28.007837899674264 24.784981972727454 68-69 18.884849073376284 28.266065153690047 29.107694342014263 23.74139143091941 70-71 22.608306698703977 27.77891983579157 26.60530888250255 23.00746458300191 72-73 21.321547134809293 24.88059553493407 30.18159601213877 23.61626131811787 74-75 19.530340544164144 30.529827181764375 27.87227295592417 22.06755931814731 76-77 21.667667495039318 25.66213445453649 28.0046924479367 24.665505602487492 78-79 21.084179367067303 26.325114424922486 29.91118581698827 22.67952039102194 80-81 22.63438881182133 27.128772574527364 28.264720261975775 21.972118351675526 82-83 21.078648529477064 25.976226692152082 30.03509924132355 22.910025537047307 84-85 20.77998050384347 26.657328200446923 29.162243544996052 23.400447750713553 86-87 21.471100904168093 27.21550096860401 28.526627316698406 22.78677081052949 88-89 21.598404291023776 26.413568689736223 27.34412354249045 24.643903476749554 90-91 21.76928010675337 24.4567569326171 28.77770697150317 24.99625598912636 92-93 22.73601480763808 27.350542116967535 28.482289399668854 21.431153675725533 94-95 22.092130815635073 26.53256962234526 28.12675605321874 23.248543508800925 96-97 19.381753804647587 25.901970546129867 30.101130187428083 24.61514546179446 98-99 21.96010886911433 27.13232142211572 26.765998543591547 24.141571165178412 100-101 22.310884103648686 24.476531164416098 28.234167881794566 24.978416850140654 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6506.0 1 4099.5 2 1790.0 3 1718.0 4 1317.5 5 1294.0 6 2097.0 7 3411.0 8 4564.5 9 4684.0 10 3559.5 11 2636.0 12 2550.5 13 2606.5 14 2577.5 15 2659.5 16 2875.0 17 2765.0 18 2502.5 19 2394.0 20 2436.5 21 2661.0 22 2968.0 23 3349.5 24 3692.0 25 4081.0 26 4792.5 27 5567.0 28 6045.0 29 6686.5 30 7480.0 31 8537.0 32 9592.0 33 10129.5 34 10992.0 35 11643.0 36 12022.0 37 13225.5 38 14573.5 39 14917.5 40 15069.0 41 16026.5 42 17483.0 43 18619.5 44 19967.5 45 22589.0 46 33689.5 47 64547.0 48 131722.0 49 134087.0 50 70300.0 51 40238.5 52 27107.5 53 23178.0 54 21033.5 55 17895.5 56 15306.0 57 13244.5 58 11846.0 59 10503.0 60 8950.0 61 7377.0 62 5913.0 63 4528.0 64 3133.5 65 2250.5 66 1574.5 67 1154.5 68 876.5 69 601.0 70 431.0 71 291.5 72 186.0 73 121.5 74 94.0 75 80.0 76 58.0 77 38.5 78 26.0 79 20.5 80 15.0 81 10.0 82 13.0 83 12.0 84 4.5 85 1.5 86 1.0 87 1.5 88 3.0 89 3.0 90 1.5 91 1.5 92 2.0 93 1.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31254624985611157 2 0.08366085083290851 3 0.09321915442929733 4 0.09743303020835047 5 0.10760799855289338 6 0.051799838844945816 7 0.0548831625857164 8 0.055705382249921886 9 0.05806926378451267 10-11 0.055756770978934736 12-13 0.05406094292151092 14-15 0.05257066978013846 16-17 0.05406094292151092 18-19 0.061974807189488744 20-21 0.06361924651789973 22-23 0.07877892157668843 24-25 0.08849139136011577 26-27 0.08309557481376724 28-29 0.07990947361497097 30-31 0.07461643452664814 32-33 0.06613729423952903 34-35 0.3086407064511355 36-37 0.48074155991514694 38-39 0.4562291361760208 40-41 0.4379861373764615 42-43 0.4655818848563582 44-45 0.475140188452747 46-47 0.4708235352156682 48-49 0.4883984805380605 50-51 0.4827457203466478 52-53 0.4830540527207249 54-55 0.4747804673496571 56-57 0.4541221982864942 58-59 0.4389111344986926 60-61 0.4000098666359705 62-63 0.46511938629524263 64-65 0.49035125224054854 66-67 0.49944705727582184 68-69 0.5110609100327244 70-71 0.5075150877308382 72-73 0.49723734192826957 74-75 0.49836789396655207 76-77 0.4739582476854517 78-79 0.45602358125996945 80-81 0.4614193978063179 82-83 0.4706179802996168 84-85 0.47072075775764255 86-87 0.469898538093437 88-89 0.47575685320090116 90-91 0.4895290325763431 92-93 0.49610678988998697 94-95 0.5031984344937593 96-97 0.49518179276775587 98-99 0.49754567430234664 100-101 0.47565407574287544 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 972976.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.149319500924758 #Duplication Level Percentage of deduplicated Percentage of total 1 69.42301519651228 20.930566658772037 2 13.974068967463054 8.426173400560028 3 6.026936772729901 5.451241271187183 4 3.20485396974719 3.8649666515086043 5 1.9289796166592394 2.9078711386715383 6 1.2335218522931155 2.23139066616946 7 0.8097578730999095 1.7089554184134923 8 0.5793531633806235 1.3973682901307103 9 0.45023210600569813 1.2216772452186018 >10 2.1164478748100968 11.260199990890134 >50 0.14144960428532038 2.9619461701658985 >100 0.08575807900747391 4.751330229459591 >500 0.006149981761491739 1.413501428913648 >1k 0.01195829786956727 9.050859171408597 >5k 0.005466650454659323 11.290190723890289 >10k+ 0.002049993920497246 11.13176154464024 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 36199 3.720441203071813 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18951 1.947735607044778 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14585 1.4990092253046323 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 14099 1.449059380704149 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12203 1.254193320287448 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 12028 1.2362072651329528 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9007 0.9257165644373551 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8881 0.9127666047261186 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8865 0.9111221653977076 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8254 0.8483251385440134 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8175 0.8402057193599842 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 7648 0.7860419989804477 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7500 0.7708309351926461 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7363 0.756750423443127 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6461 0.6640451563039581 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5757 0.5916898258538752 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5723 0.5881953922810018 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5356 0.550476065185575 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5327 0.5474955189028301 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5147 0.5289955764582066 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5106 0.5247817006791534 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5033 0.5172789462432783 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4905 0.5041234316159905 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4857 0.4991901136307576 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4739 0.4870623735837266 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4729 0.4860345990034698 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4379 0.4500624886944796 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3981 0.4091570604002565 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3814 0.39199322490996696 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3583 0.36825163210603346 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3356 0.3449211491342027 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3330 0.34224893522553484 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3112 0.3198434493759353 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3107 0.3193295620858069 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2925 0.300624064725132 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2826 0.2904490963805891 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2720 0.2795546858298663 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2511 0.2580741971024979 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2247 0.23094094818371674 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2170 0.22302708391573894 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 2037 0.2093576819983227 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2017 0.20730213283780893 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1907 0.19599661245498348 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1594 0.1638272680929437 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1570 0.16136060910032723 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1547 0.15899672756573646 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1503 0.1544745194126063 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1477 0.15180230550393845 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1453 0.14933564651132195 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1350 0.1387495683346763 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1304 0.13402180526549473 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1239 0.12734127049382513 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1187 0.12199684267648946 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1173 0.12055795826412985 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1120 0.1151107529887685 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1088 0.11182187433194653 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1007 0.10349690023186596 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 985 0.10123579615530084 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 978 0.10051635394912105 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.541661870385292E-4 0.0 14-15 0.0 0.0 0.0 2.0555491605137228E-4 0.0 16-17 0.0 0.0 0.0 3.597211030899015E-4 0.0 18-19 0.0 0.0 0.0 6.166647481541168E-4 0.0 20-21 0.0 0.0 0.0 0.001387495683346763 0.0 22-23 0.0 0.0 0.0 0.0029805462827448984 0.0 24-25 0.0 0.0 0.0 0.00519026163029715 0.0 26-27 0.0 0.0 0.0 0.008736083932183322 0.0 28-29 0.0 0.0 0.0 0.02826380095706369 0.0 30-31 0.0 0.0 0.0 0.07245810790810873 0.0 32-33 0.0 0.0 0.0 0.16896614099422802 0.0 34-35 0.0 0.0 0.0 0.32374899278091135 0.0 36-37 0.0 0.0 0.0 0.5352136126687606 0.0 38-39 0.0 0.0 0.0 0.8313154692407624 0.0 40-41 0.0 0.0 0.0 1.1484867047080298 0.0 42-43 0.0 0.0 0.0 1.4706940356185558 0.0 44-45 0.0 0.0 0.0 1.8260470967423656 0.0 46-47 0.0 0.0 0.0 2.2848970580980414 0.0 48-49 0.0 0.0 0.0 2.7103443455953693 0.0 50-51 0.0 0.0 0.0 3.1851248129450265 0.0 52-53 0.0 0.0 0.0 3.6085679400108535 0.0 54-55 0.0 0.0 0.0 4.055752659880614 0.0 56-57 0.0 0.0 0.0 4.655099406357403 0.0 58-59 0.0 0.0 0.0 5.2585058624262055 0.0 60-61 0.0 0.0 0.0 6.04177287003996 0.0 62-63 0.0 0.0 0.0 6.741841525381921 0.0 64-65 0.0 0.0 0.0 7.337950781930901 0.0 66-67 0.0 0.0 0.0 8.046755521205045 0.0 68-69 0.0 0.0 0.0 8.870671013467959 0.0 70-71 0.0 0.0 0.0 9.74104191675848 0.0 72-73 0.0 0.0 0.0 10.572305997270231 0.0 74-75 0.0 0.0 0.0 11.504805873937281 0.0 76-77 0.0 0.0 0.0 12.278000690664518 0.0 78-79 0.0 0.0 0.0 13.401820805446384 0.0 80-81 0.0 0.0 0.0 14.570554669385473 0.0 82-83 0.0 0.0 0.0 15.540516929502886 0.0 84-85 0.0 0.0 0.0 16.772561707585798 0.0 86-87 0.0 0.0 0.0 17.999056502935325 0.0 88-89 0.0 0.0 0.0 19.154377908602065 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCGAT 35 2.8345693E-4 54.102867 7 GGACCGA 80 7.2759576E-11 53.257507 6 GGGACCG 75 3.132996E-4 31.571367 5 GTAAGCG 280 0.0 31.427675 94-95 CTACACT 155 1.4006218E-9 30.552937 4 CTTTGCG 790 0.0 29.373362 2 TTTGCGT 800 0.0 29.006195 3 GTCTAAT 100 4.9829054E-5 28.474272 1 TCACGTA 35 0.008426843 27.120451 28-29 CAACCGT 140 5.9781814E-7 27.051434 6 GCTTTGC 915 0.0 25.932852 1 GGATAGG 95 0.0012507772 24.915794 6 TACACCG 100 0.0016827232 23.678526 5 AACCGTG 160 1.9144281E-6 23.670004 7 GCTTATA 1090 0.0 23.510868 1 GAGTACT 4170 0.0 22.875326 12-13 ATTCGCG 75 3.0075285E-5 22.192408 80-81 TTGCGTT 1100 0.0 21.956451 4 ACTATCC 110 0.002927805 21.525932 3 CCTATAA 310 1.0913936E-11 21.432247 1 >>END_MODULE