##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939055_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 972976 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.98791439871076 33.0 31.0 34.0 30.0 34.0 2 32.194750949663714 34.0 31.0 34.0 30.0 34.0 3 32.2952169426584 34.0 31.0 34.0 30.0 34.0 4 35.81073428327112 37.0 35.0 37.0 35.0 37.0 5 35.7532117955633 37.0 35.0 37.0 33.0 37.0 6 35.861010960188125 37.0 35.0 37.0 35.0 37.0 7 35.805251105885446 37.0 35.0 37.0 33.0 37.0 8 35.81901095196593 37.0 35.0 37.0 33.0 37.0 9 37.39749490223808 39.0 37.0 39.0 34.0 39.0 10-11 37.29745389403233 39.0 37.0 39.0 33.5 39.0 12-13 37.242137524461036 39.0 37.0 39.0 33.0 39.0 14-15 38.2788239381033 40.0 37.5 41.0 33.5 41.0 16-17 38.47196436499975 40.0 38.0 41.0 34.0 41.0 18-19 38.59963400947197 40.0 38.0 41.0 34.0 41.0 20-21 38.55801427784447 40.0 38.0 41.0 34.0 41.0 22-23 38.50707057522487 40.0 38.0 41.0 34.0 41.0 24-25 38.35607815609018 40.0 38.0 41.0 33.5 41.0 26-27 38.0573462243673 40.0 37.0 41.0 33.5 41.0 28-29 37.90032950453043 40.0 37.0 41.0 33.0 41.0 30-31 37.79308842150269 40.0 37.0 41.0 33.0 41.0 32-33 37.65488151814639 40.0 36.0 41.0 33.0 41.0 34-35 37.46536964940553 40.0 36.0 41.0 32.5 41.0 36-37 37.25803616944303 39.5 35.5 41.0 31.5 41.0 38-39 37.03647828928977 39.0 35.0 41.0 31.0 41.0 40-41 36.95373421338245 39.0 35.0 41.0 31.0 41.0 42-43 36.77817952344148 39.0 35.0 41.0 31.0 41.0 44-45 36.54751196329612 39.0 35.0 41.0 30.0 41.0 46-47 36.19772892651001 39.0 35.0 41.0 30.0 41.0 48-49 35.79924993011133 39.0 35.0 40.0 28.0 41.0 50-51 35.5183241929914 39.0 35.0 40.0 28.0 41.0 52-53 35.26719107151666 38.0 34.5 40.0 27.0 41.0 54-55 35.045874204502475 38.0 34.0 40.0 26.5 41.0 56-57 34.91576205374028 38.0 34.0 40.0 26.5 41.0 58-59 34.675887688904965 38.0 34.0 40.0 26.0 41.0 60-61 34.160689472299424 37.5 33.5 40.0 25.0 41.0 62-63 33.7561168004144 37.0 33.0 39.5 23.5 41.0 64-65 33.27175541842759 36.0 32.5 39.0 22.0 40.5 66-67 32.723721345644705 36.0 32.0 39.0 22.0 40.0 68-69 32.1956985578267 35.0 31.5 38.0 20.5 39.5 70-71 31.506464702109817 35.0 31.0 37.0 18.5 39.0 72-73 30.889472607751888 34.5 30.5 36.5 15.5 39.0 74-75 30.398629565374684 34.0 30.0 36.0 12.0 38.0 76-77 28.485289976320075 31.5 27.0 34.0 7.5 36.0 78-79 29.441717472990085 34.0 29.0 35.0 7.0 36.0 80-81 29.404509463748333 34.0 29.0 35.0 3.5 36.0 82-83 28.974065650129088 34.0 29.0 35.0 2.0 36.0 84-85 28.5419321750999 33.0 29.0 35.0 2.0 35.0 86-87 27.95061748696782 33.0 27.5 35.0 2.0 35.0 88-89 27.174705748137672 33.0 25.5 35.0 2.0 35.0 90-91 26.4971576893983 32.0 25.0 34.0 2.0 35.0 92-93 26.135354315008797 32.0 24.0 34.0 2.0 35.0 94-95 25.556011145187547 32.0 23.5 34.0 2.0 35.0 96-97 24.572143094999262 31.0 18.0 34.0 2.0 35.0 98-99 23.367256746312343 31.0 2.0 34.0 2.0 35.0 100-101 18.01065493907352 23.5 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10545973602924619 1101 2 0.19995180142952051 1101 3 0.20860866733443117 1101 4 0.002731323902288807 1101 5 0.05424406456816655 1101 6 0.13211988499534044 1101 7 0.13144574972920964 1101 8 0.10892630207197129 1101 9 0.20534425793977817 1101 10-11 0.20197009078953698 1101 12-13 0.13472191540820688 1101 14-15 0.2293931840490373 1101 16-17 0.048437208250625474 1101 18-19 0.29451186949029307 1101 20-21 0.31235666268484863 1101 22-23 0.23905708113525037 1101 24-25 0.1644695723256504 1101 26-27 -0.055813265540301416 1101 28-29 0.14651386397818555 1101 30-31 0.15439561339555752 1101 32-33 0.18645971579455534 1101 34-35 0.3590590302264829 1101 36-37 0.5465442641625273 1101 38-39 0.4043608105304699 1101 40-41 0.5573683215902534 1101 42-43 0.5164312656605503 1101 44-45 0.4727283629177208 1101 46-47 0.39993232973923654 1101 48-49 0.3682011635780498 1101 50-51 0.47418790030366864 1101 52-53 0.5246058877511288 1101 54-55 0.07618488425408287 1101 56-57 0.3268836838394833 1101 58-59 0.3416247333719227 1101 60-61 0.37562919624667046 1101 62-63 0.2607109902497555 1101 64-65 0.30810827423335496 1101 66-67 0.27763387086201163 1101 68-69 0.2269062372362214 1101 70-71 0.582406992244799 1101 72-73 0.2497843412048475 1101 74-75 0.3369975874003721 1101 76-77 0.1163648245827602 1101 78-79 0.3106841566239815 1101 80-81 0.08614224928360059 1101 82-83 -0.038246613571427446 1101 84-85 0.4420838820561883 1101 86-87 0.5740602735505789 1101 88-89 0.5060166601319835 1101 90-91 0.48716137964942163 1101 92-93 0.30102430135782754 1101 94-95 0.1531187274525081 1101 96-97 0.5933768748650223 1101 98-99 0.6147290457837684 1101 100-101 0.9832085071852497 1102 1 0.2807059924665225 1102 2 0.2616050124481717 1102 3 0.24703345258642173 1102 4 0.1134627842920608 1102 5 0.20815756618749504 1102 6 0.16272179926142627 1102 7 0.22010839778007352 1102 8 0.1817159342498229 1102 9 0.2886556386803818 1102 10-11 0.32485166559104783 1102 12-13 0.4061437719260681 1102 14-15 0.4837217188038707 1102 16-17 0.33049703293980315 1102 18-19 0.3453192083711727 1102 20-21 0.36497057081453477 1102 22-23 0.3948985369278901 1102 24-25 0.4913449694093188 1102 26-27 0.4967916032864963 1102 28-29 0.4568228015243463 1102 30-31 0.39035499335103196 1102 32-33 0.4874917459186747 1102 34-35 0.5427492868910306 1102 36-37 0.608478984899719 1102 38-39 0.6601123898258194 1102 40-41 0.662016331135753 1102 42-43 0.6379569450287264 1102 44-45 0.5076691425705064 1102 46-47 0.660696848150998 1102 48-49 0.6728863633982982 1102 50-51 0.6238994174086088 1102 52-53 0.8543131692970078 1102 54-55 0.8074502833522175 1102 56-57 0.8790223829030168 1102 58-59 0.7383029186931296 1102 60-61 0.8622342406942138 1102 62-63 0.87556308142792 1102 64-65 0.9800280672074919 1102 66-67 0.5060867554326904 1102 68-69 0.5412441212128911 1102 70-71 0.7780200894198828 1102 72-73 0.8879035980191894 1102 74-75 0.8692614420690425 1102 76-77 0.5619365992303571 1102 78-79 0.8048654282214578 1102 80-81 0.8479488701855651 1102 82-83 0.7838676535477234 1102 84-85 0.9033930830334675 1102 86-87 1.1689936151484162 1102 88-89 0.9438624134674889 1102 90-91 0.9530883730739781 1102 92-93 1.0874578573162772 1102 94-95 1.5992845134328064 1102 96-97 1.8249457362744295 1102 98-99 2.05391132461785 1102 100-101 2.27935543976238 1103 1 -0.11236040635251143 1103 2 -0.054508457548330114 1103 3 0.014607148731549557 1103 4 0.13769842933307785 1103 5 0.08780875552228906 1103 6 0.07391356903421098 1103 7 0.10997127805522666 1103 8 -0.0020336282149884255 1103 9 0.11577216761858011 1103 10-11 -0.041289197913179976 1103 12-13 -0.08555066871372219 1103 14-15 -0.05441993606837059 1103 16-17 0.01499991686742419 1103 18-19 0.11022584216563303 1103 20-21 0.007123852708957656 1103 22-23 0.11580054426326569 1103 24-25 0.1417809827408263 1103 26-27 0.0656520333645716 1103 28-29 0.12444443630801771 1103 30-31 0.30480354682163835 1103 32-33 0.24355255950585075 1103 34-35 0.09346532274609132 1103 36-37 -0.05244265444952845 1103 38-39 -0.10090588773129383 1103 40-41 0.3215720997519256 1103 42-43 0.2946668325631592 1103 44-45 0.28119712199494273 1103 46-47 0.09957127935525278 1103 48-49 -0.13411496062123973 1103 50-51 0.18244543089230092 1103 52-53 0.3391554283482847 1103 54-55 0.49610023997916386 1103 56-57 0.4565764470342657 1103 58-59 0.2482026280175731 1103 60-61 0.040330087534066195 1103 62-63 0.31238680420561593 1103 64-65 0.2723510366637285 1103 66-67 0.11709894554363842 1103 68-69 -0.10588564792070798 1103 70-71 0.15748865536975387 1103 72-73 0.21878292961759982 1103 74-75 0.14385091991834997 1103 76-77 -0.24160248978332532 1103 78-79 -0.1977369550137169 1103 80-81 -0.09545309910327049 1103 82-83 0.05841908485850311 1103 84-85 0.2580781337875493 1103 86-87 0.4526115365899237 1103 88-89 -4.4886809211774903E-4 1103 90-91 -0.13499697292124324 1103 92-93 -0.2839448424660951 1103 94-95 -0.24612859588250657 1103 96-97 -0.15217688010993058 1103 98-99 0.07382868753617089 1103 100-101 0.6848934939102129 1104 1 0.017866655719529945 1104 2 0.05912691347177912 1104 3 0.03051459630927411 1104 4 0.09743482574783258 1104 5 0.06863537987262447 1104 6 0.00945152513586578 1104 7 0.12407591392608452 1104 8 0.10155646626884618 1104 9 0.2848312600496783 1104 10-11 0.1544614560003481 1104 12-13 0.2191414945147372 1104 14-15 0.33188281983140655 1104 16-17 0.33970013572827185 1104 18-19 0.20712879120647187 1104 20-21 0.46253034671332927 1104 22-23 0.37514580575589207 1104 24-25 0.19792413035786183 1104 26-27 0.24936291380636533 1104 28-29 0.1883981503913006 1104 30-31 0.14493563978169988 1104 32-33 0.319445351655979 1104 34-35 0.3738777614189459 1104 36-37 0.36204389604132103 1104 38-39 0.31792027075114504 1104 40-41 0.37764488190530443 1104 42-43 0.3456526769729962 1104 44-45 0.4705542242966203 1104 46-47 0.6726409605754355 1104 48-49 0.39808815778199147 1104 50-51 0.4259244935272761 1104 52-53 0.5189877159052401 1104 54-55 0.7272010469982391 1104 56-57 0.7047032698637281 1104 58-59 0.8064530999514616 1104 60-61 0.5508375923747266 1104 62-63 0.6059333206298803 1104 64-65 0.8791124298028592 1104 66-67 0.6577313612142675 1104 68-69 0.531359762552583 1104 70-71 0.5323400386577077 1104 72-73 0.5518809026069675 1104 74-75 0.830515936636214 1104 76-77 0.7469910259178789 1104 78-79 0.9528172336724872 1104 80-81 0.9761073642411766 1104 82-83 0.9228733642918918 1104 84-85 1.2437344979797764 1104 86-87 1.2416777140952995 1104 88-89 1.3747579821070879 1104 90-91 1.219725034969393 1104 92-93 1.3655344531028213 1104 94-95 1.4629890697311048 1104 96-97 1.3544192755093576 1104 98-99 1.3484020059229138 1104 100-101 1.2502557304643425 1105 1 -0.10530482211571979 1105 2 -0.09737693038470496 1105 3 -0.070119718604019 1105 4 -0.04725813729842088 1105 5 -0.1613962754160383 1105 6 0.041770631726407714 1105 7 -0.055898549034324674 1105 8 0.050901958715350304 1105 9 -0.03319194093117517 1105 10-11 -0.0862825762885322 1105 12-13 0.01306290524271958 1105 14-15 -0.18851718790708816 1105 16-17 0.021614059460553392 1105 18-19 -0.049432003150229775 1105 20-21 0.12498316001967424 1105 22-23 0.10140131641203709 1105 24-25 -0.005464122120265813 1105 26-27 0.025026308539835895 1105 28-29 0.056256666486163454 1105 30-31 -0.11812186450470818 1105 32-33 -0.05331320482416402 1105 34-35 0.11317868496018235 1105 36-37 0.15131072183083205 1105 38-39 0.12826966388371375 1105 40-41 0.20959556349760078 1105 42-43 0.06354033334503839 1105 44-45 0.2961780801477687 1105 46-47 0.21999693384859853 1105 48-49 0.1868785823594621 1105 50-51 0.11545479095155997 1105 52-53 0.27690093349968237 1105 54-55 0.3443135236316053 1105 56-57 0.10412457539651143 1105 58-59 0.10682345957391703 1105 60-61 0.059583302950109385 1105 62-63 -0.04971505658314612 1105 64-65 -0.33764649425588544 1105 66-67 0.016182767081495797 1105 68-69 0.10446316579839987 1105 70-71 0.024738174647286115 1105 72-73 0.0053870462479785886 1105 74-75 0.056668065811070534 1105 76-77 -0.49668093920084644 1105 78-79 -0.22373899013451393 1105 80-81 -0.1350519109518622 1105 82-83 0.07211873993545481 1105 84-85 0.04231230735641489 1105 86-87 0.07263690077927976 1105 88-89 0.14381463515883652 1105 90-91 -0.029281051842030337 1105 92-93 0.11054312263756927 1105 94-95 0.29340474792571314 1105 96-97 0.05481248788031223 1105 98-99 0.04207481743972963 1105 100-101 -0.41079085286838435 1106 1 -0.11456180581359732 1106 2 -0.019146691854423636 1106 3 -0.03225121122585506 1106 4 -0.06101360903416975 1106 5 -0.18913873252368063 1106 6 -0.08315056459359482 1106 7 -0.04101756485204788 1106 8 -0.09335596884581321 1106 9 -0.11033228449741017 1106 10-11 0.10899205231017106 1106 12-13 -0.06015595350191205 1106 14-15 -0.07069099409812196 1106 16-17 -0.11653012723204625 1106 18-19 -0.07688417777561796 1106 20-21 -0.054860194292359665 1106 22-23 -0.10650149060175806 1106 24-25 -0.0176257115148033 1106 26-27 -0.015944481562769397 1106 28-29 -0.08797989058550826 1106 30-31 0.1115539095522351 1106 32-33 0.24213737413801084 1106 34-35 -0.008512005698428027 1106 36-37 -0.05350583032562639 1106 38-39 0.014703320979961632 1106 40-41 0.1132182176411618 1106 42-43 0.17346656953881023 1106 44-45 0.10395408892391345 1106 46-47 0.3374064031124959 1106 48-49 0.4388010359765886 1106 50-51 0.43392672971798163 1106 52-53 0.3608442162205705 1106 54-55 0.43615320221944387 1106 56-57 0.4983869551429905 1106 58-59 0.18775884589894076 1106 60-61 0.2512480996989481 1106 62-63 0.4986695124987435 1106 64-65 0.767540835893243 1106 66-67 0.7003500837672263 1106 68-69 0.4838303143695981 1106 70-71 0.613124738308187 1106 72-73 0.4746491588633326 1106 74-75 0.39619884109681536 1106 76-77 0.5288122738878656 1106 78-79 0.8561955449326781 1106 80-81 0.504864245494371 1106 82-83 0.1844349092039792 1106 84-85 0.29201935356297426 1106 86-87 0.5707598594523162 1106 88-89 0.6955739607061879 1106 90-91 0.7706555490391445 1106 92-93 0.8511303124538152 1106 94-95 0.786801266672974 1106 96-97 0.8856739679291792 1106 98-99 0.8989103465908599 1106 100-101 1.0944431361828002 1107 1 -0.06517568313122268 1107 2 -0.053590090914021005 1107 3 0.013219705096446432 1107 4 0.02958025451983559 1107 5 0.04550103635391878 1107 6 -0.010636687386785582 1107 7 0.144098666797845 1107 8 0.11533572278264614 1107 9 0.0541717653530398 1107 10-11 0.020432256481591082 1107 12-13 -0.004011584007500346 1107 14-15 0.03191001944497174 1107 16-17 0.19710305793738314 1107 18-19 0.15660917701211474 1107 20-21 0.12652257281547463 1107 22-23 0.18209288049952477 1107 24-25 0.19978183010572792 1107 26-27 0.14124501420342028 1107 28-29 0.23456695503035974 1107 30-31 0.049203721881646345 1107 32-33 0.3795790699221513 1107 34-35 0.20264095774598445 1107 36-37 0.4013811960790221 1107 38-39 0.48918133963952215 1107 40-41 0.45782674687333014 1107 42-43 0.5301952833054244 1107 44-45 0.47399649904205887 1107 46-47 0.2480143627392195 1107 48-49 0.5087539050039069 1107 50-51 0.4918209183017197 1107 52-53 0.44982733750331505 1107 54-55 0.16528863201113353 1107 56-57 0.1546322800009463 1107 58-59 0.3242530462294013 1107 60-61 0.3319010182619877 1107 62-63 0.5258936611103664 1107 64-65 0.3340084731055484 1107 66-67 0.3571400799883193 1107 68-69 0.3513649090354747 1107 70-71 0.29704080905008823 1107 72-73 0.3053666290172288 1107 74-75 0.142254544964036 1107 76-77 0.2766216754807651 1107 78-79 0.519850173420263 1107 80-81 0.6919735421424527 1107 82-83 0.5269711384380393 1107 84-85 0.6818344977540569 1107 86-87 0.8039484642403281 1107 88-89 0.9102664568746519 1107 90-91 1.29404261810879 1107 92-93 1.4974745868662325 1107 94-95 1.433689104266712 1107 96-97 1.5062879228195278 1107 98-99 1.3021062321334078 1107 100-101 1.4273376295165328 1108 1 0.08747667175273222 1108 2 0.1183978745205394 1108 3 0.09105642409386405 1108 4 0.01032268767370681 1108 5 -0.03391578489206637 1108 6 0.1374506763194958 1108 7 0.11851616668473497 1108 8 0.1731706320709776 1108 9 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-0.8521083724342304 2316 74-75 -1.1096593680289537 2316 76-77 -0.9173550029613082 2316 78-79 -1.003675928235289 2316 80-81 -1.1453690864677704 2316 82-83 -1.1854412717391298 2316 84-85 -1.3707823668317438 2316 86-87 -1.5334812517654441 2316 88-89 -1.63375867788751 2316 90-91 -1.7980395745285271 2316 92-93 -2.074389485340383 2316 94-95 -1.8940422020888796 2316 96-97 -1.6085647208411764 2316 98-99 -1.9248703167940775 2316 100-101 -0.9677877093427547 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 15.0 5 61.0 6 176.0 7 223.0 8 442.0 9 749.0 10 1266.0 11 1901.0 12 2767.0 13 3718.0 14 4382.0 15 5643.0 16 7201.0 17 9953.0 18 9856.0 19 7010.0 20 7100.0 21 7611.0 22 8454.0 23 9384.0 24 10266.0 25 12196.0 26 14013.0 27 16968.0 28 20135.0 29 24340.0 30 29083.0 31 35513.0 32 43421.0 33 54995.0 34 74539.0 35 106392.0 36 153556.0 37 192613.0 38 93791.0 39 3236.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.39459148280281 18.49712056090772 14.21747554385393 18.890812412435544 2 14.562846360033546 25.465890217230434 35.13169903471412 24.839564388021902 3 26.893469109207214 25.025386032132346 30.681640657117953 17.399504201542484 4 12.224967522323265 15.52504892207002 40.49072125108944 31.75926230451728 5 11.169443028399467 41.37296295078193 34.130338261169854 13.327255759648748 6 27.544769860715988 37.18036210554011 16.37553238723257 18.89933564651132 7 24.484982157833286 36.06563779579353 21.050981730279062 18.398398316094127 8 23.386393908996727 28.123304171942575 21.000415220930424 27.489886698130274 9 22.27280015128842 22.242378023712813 24.576967982766277 30.90785384223249 10-11 24.91736692374735 24.1533193007844 27.054521385933466 23.87479238953479 12-13 31.548671293022647 22.863924701123153 25.616767525612143 19.970636480242064 14-15 24.48715932671643 25.41920915800498 30.662625403021494 19.431006112257094 16-17 19.32444904897868 28.594671484982847 30.82869282617678 21.2521866398617 18-19 18.42456059931776 32.179156050579 30.120928329974213 19.27535502012903 20-21 21.890005508871752 26.69022668596142 27.063411636052688 24.35635616911414 22-23 19.63404030520794 26.939256466757662 28.47022948150828 24.95647374652612 24-25 24.127984657381067 26.728254345430923 28.13697357385999 21.006787423328017 26-27 23.62992509578859 22.984534048116295 33.42086546841854 19.96467538767657 28-29 19.426429839996054 29.909987502261103 30.847317919455364 19.81626473828748 30-31 20.352763069181563 27.59086555064051 29.092803933498875 22.963567446679054 32-33 20.702309203926923 27.990669862360427 29.824682212099784 21.482338721612866 34-35 23.731074953924086 25.24148096676675 29.12193131322268 21.90551276608648 36-37 22.677311945458452 24.58820904989191 32.630764122401864 20.103714882247782 38-39 22.51242330035015 25.90588974163544 32.03476930873052 19.546917649283895 40-41 20.68480133864447 27.730312064296868 31.053077602630008 20.53180899442865 42-43 19.075421664456847 29.40694602900517 27.86670161265456 23.650930693883424 44-45 21.203165397085282 26.54758656201678 27.82790838458167 24.421339656316277 46-47 23.01127531112325 28.46428936659176 27.33639852965133 21.188036792633664 48-49 22.692997706449127 26.839632846855245 28.603349314016047 21.864020132679578 50-51 19.303749656977768 28.45296201626157 31.936269295321846 20.307019031438823 52-53 21.086234398381873 30.01168579697752 27.70345825590765 21.19862154873296 54-55 17.985952377037048 26.674347568696454 30.321713999112003 25.017986055154495 56-57 22.10599233691273 27.07137688904968 27.64456677245893 23.17806400157866 58-59 24.469059860683938 26.018803300213005 27.275320090342177 22.23681674876088 60-61 20.810105245847723 26.681209916132214 31.586241983226444 20.92244285479362 62-63 21.245971090518907 26.44912268440483 30.173397275141628 22.131508949934634 64-65 19.885608277726245 30.129577146322223 28.834011238755465 21.150803337196066 66-67 20.454741671702905 26.836172128417964 27.62331594288698 25.085770256992152 68-69 18.79496005435034 28.503762280987555 28.591793797606652 24.109483867055452 70-71 22.296798687737414 28.08630428705333 26.201674039236323 23.41522298597293 72-73 21.01536934107316 25.201700761375413 29.654534130338263 24.128395767213167 74-75 19.14980431171992 30.818848563582247 27.137462794560196 22.89388433013764 76-77 21.208316595592347 25.86810368638351 27.51849209627154 25.4050876217526 78-79 20.65171809862108 26.425787513258836 29.32284343471719 23.599650953402897 80-81 22.10384536917939 27.12334483242384 27.784923669712136 22.987886128684636 82-83 20.506466757658977 26.369715183108315 29.44409728503067 23.679720774202035 84-85 19.927510909849595 26.845938329287318 28.49223103597835 24.734319724884735 86-87 20.637505361920645 27.48610529629176 27.828805317790877 24.047584023996716 88-89 20.522201080547717 26.58994767564577 26.664416600738704 26.22343464306781 90-91 20.69553717098493 24.62367882032472 28.168522976443526 26.512261032246816 92-93 21.533110785877554 27.614452977257404 27.78948298827515 23.062953248589892 94-95 20.650966269223378 26.5824502116717 27.659257386412108 25.10732613269282 96-97 17.63481319169229 26.51992443801286 29.572003831543636 26.273258538751215 98-99 20.393308776372695 27.241627748269227 26.03157734620381 26.333486129154267 100-101 20.098379290604328 24.955985236939718 27.91142369386897 27.034211778586986 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5522.0 1 3613.5 2 1662.0 3 1564.0 4 1279.5 5 1227.0 6 2040.5 7 3349.5 8 4346.5 9 4444.0 10 3490.0 11 2574.0 12 2438.0 13 2423.5 14 2368.5 15 2339.5 16 2314.5 17 2282.5 18 2235.0 19 2316.0 20 2443.5 21 2606.5 22 2941.0 23 3359.5 24 3621.0 25 4018.0 26 4770.5 27 5437.0 28 5987.5 29 6712.5 30 7439.0 31 8338.5 32 9457.5 33 10228.5 34 11045.0 35 11639.0 36 12108.0 37 13326.0 38 14462.0 39 14839.5 40 15021.0 41 15956.5 42 17375.5 43 18567.5 44 20211.0 45 22570.5 46 31459.5 47 64700.5 48 131997.0 49 127359.5 50 62748.0 51 42840.0 52 38342.5 53 30734.5 54 22622.0 55 17582.5 56 14964.5 57 13053.5 58 11807.5 59 10630.5 60 9099.5 61 7388.0 62 5869.0 63 4433.0 64 2997.5 65 2154.0 66 1481.0 67 1070.0 68 724.5 69 454.0 70 339.0 71 212.0 72 115.5 73 67.5 74 57.0 75 48.0 76 30.0 77 15.5 78 8.5 79 8.5 80 6.0 81 2.0 82 0.5 83 0.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.37924882011478184 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0013361069543339197 16-17 0.002261104076565095 18-19 3.083323740770584E-4 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.012847182253210767 36-37 0.021891598559471148 38-39 0.010945799279735574 40-41 0.005293039088322836 42-43 0.004727763069181563 44-45 0.0017986055154495077 46-47 9.763858512440183E-4 48-49 0.0031347124697834274 50-51 7.19442206179803E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.736083932183322E-4 60-61 0.0016444393284109785 62-63 8.222196642054892E-4 64-65 3.597211030899015E-4 66-67 0.002877768824719212 68-69 0.002980546282744898 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.08957055466938547 78-79 0.004111098321027446 80-81 5.138872901284308E-5 82-83 0.0 84-85 2.055549160513723E-4 86-87 0.08664139711565343 88-89 0.42298062850471135 90-91 0.3725168966140994 92-93 0.0 94-95 7.19442206179803E-4 96-97 0.0 98-99 0.0 100-101 7.19442206179803E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 972976.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.85310506333289 #Duplication Level Percentage of deduplicated Percentage of total 1 67.75050499077415 19.54812438592666 2 14.739811525107779 8.505786610953194 3 6.360207337717967 5.505351916192718 4 3.388251922283328 3.910463547787219 5 2.0071081510059283 2.8955651177222936 6 1.2445920739805483 2.154620752293128 7 0.8746480117273492 1.7665417683063098 8 0.6472359430572301 1.4939813332636485 9 0.4645542412962517 1.2063449098563885 >10 2.251170154606613 11.486907513969092 >50 0.1493219676082046 3.0013831892105616 >100 0.09479627645937287 4.998568967061228 >500 0.0071275395834112945 1.5253182765034372 >1k 0.012829571250140332 9.410413781113764 >5k 0.0057020316667290355 11.500451484242308 >10k+ 0.002138261875023388 11.090176445598065 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 37566 3.860937988192926 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18704 1.9223495749124335 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14010 1.4399121869398628 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 13493 1.3867762411405833 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 12109 1.2445322392330336 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 11972 1.2304517274835145 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9589 0.9855330450083044 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 9090 0.934247093453487 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9025 0.9275665586818175 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8778 0.9021805265494729 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8433 0.8667223035306112 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8002 0.8224252191215407 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7530 0.7739142589334167 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 7043 0.7238616368749076 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6345 0.6521229711729786 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5956 0.6121425400009867 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5768 0.5928203778921577 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5330 0.5478038512769071 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5313 0.5460566344904705 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5302 0.544926082452188 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 5191 0.5335177846113368 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5149 0.529201131374258 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4943 0.5080289750209666 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4918 0.5054595385703244 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4886 0.5021706599135024 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4767 0.48994014240844586 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4592 0.4719540872539508 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4412 0.4534541448093273 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3726 0.38294880860370656 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3664 0.37657660620611405 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3538 0.3636266464948776 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3352 0.34451003930209995 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3248 0.33382118366742863 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3103 0.3189184522537041 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3079 0.31645179326108763 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2910 0.2990824028547467 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2826 0.2904490963805891 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2458 0.25262699182713655 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2452 0.2520103270789824 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2214 0.22754929206886912 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2057 0.2114132311588364 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2033 0.20894657216621992 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1800 0.18499942444623504 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1679 0.17256335202512704 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1642 0.16876058607817665 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1615 0.16598559471148314 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1598 0.16423837792504645 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1546 0.15889395010771076 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1494 0.1535495222903751 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1355 0.13926345562480472 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1349 0.1386467908766506 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1292 0.13278847576918648 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1264 0.12991070694446727 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1261 0.12960237457039023 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1172 0.12045518080610415 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1115 0.11459686569864004 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1098 0.11284964891220338 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1060 0.10894410550722732 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.541661870385292E-4 5.138872901284307E-5 14-15 0.0 0.0 0.0 2.0555491605137228E-4 1.0277745802568614E-4 16-17 0.0 0.0 0.0 3.597211030899015E-4 1.0277745802568614E-4 18-19 0.0 0.0 0.0 6.166647481541168E-4 1.0277745802568614E-4 20-21 0.0 0.0 0.0 0.001387495683346763 1.0277745802568614E-4 22-23 0.0 0.0 0.0 0.003031935011757741 1.0277745802568614E-4 24-25 0.0 0.0 0.0 0.005293039088322836 1.0277745802568614E-4 26-27 0.0 0.0 0.0 0.008787472661196165 1.0277745802568614E-4 28-29 0.0 0.0 0.0 0.02810963477002516 1.0277745802568614E-4 30-31 0.0 0.0 0.0 0.07317755011428853 1.0277745802568614E-4 32-33 0.0 0.0 0.0 0.1713300225288188 1.0277745802568614E-4 34-35 0.0 0.0 0.0 0.32806564601799015 1.0277745802568614E-4 36-37 0.0 0.0 0.0 0.5417913699824045 1.0277745802568614E-4 38-39 0.0 0.0 0.0 0.8400515531729457 1.0277745802568614E-4 40-41 0.0 0.0 0.0 1.1612311095032148 1.0277745802568614E-4 42-43 0.0 0.0 0.0 1.4864703754254989 1.0277745802568614E-4 44-45 0.0 0.0 0.0 1.8445470391869891 1.0277745802568614E-4 46-47 0.0 0.0 0.0 2.306942822844551 1.0277745802568614E-4 48-49 0.0 0.0 0.0 2.7341887158573286 1.0277745802568614E-4 50-51 0.0 0.0 0.0 3.21338861390209 1.0277745802568614E-4 52-53 0.0 0.0 0.0 3.638784512670405 1.0277745802568614E-4 54-55 0.0 0.0 0.0 4.09002894213218 1.0277745802568614E-4 56-57 0.0 0.0 0.0 4.6949256713423555 1.0277745802568614E-4 58-59 0.0 0.0 0.0 5.303316834125405 1.0277745802568614E-4 60-61 0.0 0.0 0.0 6.094086596175034 1.0277745802568614E-4 62-63 0.0 0.0 0.0 6.800219121540511 1.0277745802568614E-4 64-65 0.0 0.0 0.0 7.400182532765454 1.0277745802568614E-4 66-67 0.0 0.0 0.0 8.113920590024831 1.0277745802568614E-4 68-69 0.0 0.0 0.0 8.944465228330401 1.0277745802568614E-4 70-71 0.0 0.0 0.0 9.821054167831477 1.0277745802568614E-4 72-73 0.0 0.0 0.0 10.657765453618588 1.0277745802568614E-4 74-75 0.0 0.0 0.0 11.597408363618424 1.0277745802568614E-4 76-77 0.0 0.0 0.0 12.376923994014241 1.0277745802568614E-4 78-79 0.0 0.0 0.0 13.50536909440726 1.0277745802568614E-4 80-81 0.0 0.0 0.0 14.675233510384635 1.0277745802568614E-4 82-83 0.0 0.0 0.0 15.648124928055779 1.0277745802568614E-4 84-85 0.0 0.0 0.0 16.88232803275723 1.0277745802568614E-4 86-87 0.0 0.0 0.0 18.107024222591306 1.0277745802568614E-4 88-89 0.0 0.0 0.0 19.264658121063626 1.0277745802568614E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 365 0.0 35.10671 94-95 GCACCGT 85 6.440779E-4 27.915663 6 CTACGTT 70 0.008424196 27.12086 9 ATAGGAC 105 6.947837E-5 27.118073 3 CTCCGAC 325 0.0 26.28502 94-95 CTTTGCG 865 0.0 25.78568 2 TTTGCGT 875 0.0 25.490988 3 TATAGAG 170 1.13243004E-7 25.124098 5 GTGTAAG 155 1.3807003E-6 24.575834 1 ATGGGAG 730 0.0 23.403269 5 ATGGGGA 460 0.0 22.696648 5 CATGGGG 1160 0.0 22.500988 4 TTAGGGT 170 3.1732216E-6 22.332531 4 ACTTACC 150 3.0540607E-5 22.146425 8 GAGTACT 4695 0.0 22.090319 12-13 GTACATG 5195 0.0 21.44768 1 TAGTACT 155 3.933238E-5 21.432026 4 AGTACTT 4760 0.0 21.19035 12-13 TACATGG 5110 0.0 21.081516 2 TCAACGA 215 0.0 20.970285 94-95 >>END_MODULE