##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939054_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 981708 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.801089529676847 33.0 31.0 34.0 30.0 34.0 2 31.77441459171159 33.0 31.0 34.0 30.0 34.0 3 31.84097715410285 33.0 31.0 34.0 30.0 34.0 4 35.31978246077245 37.0 35.0 37.0 33.0 37.0 5 35.40017703838616 37.0 35.0 37.0 33.0 37.0 6 35.5529098265472 37.0 35.0 37.0 33.0 37.0 7 35.49833351668725 37.0 35.0 37.0 33.0 37.0 8 35.51477730649032 37.0 35.0 37.0 33.0 37.0 9 37.0467226507271 39.0 37.0 39.0 33.0 39.0 10-11 36.94875054496856 39.0 37.0 39.0 33.0 39.0 12-13 36.87032956846639 39.0 37.0 39.0 33.0 39.0 14-15 37.878220407697604 40.0 37.5 41.0 33.0 41.0 16-17 37.883132255212345 40.0 37.5 41.0 33.0 41.0 18-19 37.915513574301116 40.0 38.0 41.0 33.0 41.0 20-21 37.771553761403595 40.0 37.0 41.0 32.5 41.0 22-23 37.89585956312875 40.0 38.0 41.0 33.0 41.0 24-25 37.77849981868336 40.0 37.5 41.0 32.5 41.0 26-27 37.58553001503502 40.0 37.0 41.0 32.5 41.0 28-29 37.485078556963984 40.0 37.0 41.0 32.0 41.0 30-31 37.396770730196764 40.0 37.0 41.0 32.0 41.0 32-33 37.18635021819115 40.0 36.0 41.0 31.5 41.0 34-35 36.803234261104116 39.0 35.0 41.0 30.5 41.0 36-37 36.458357271204875 39.0 35.0 41.0 30.0 41.0 38-39 36.18214428322882 39.0 35.0 40.5 30.0 41.0 40-41 36.11579410578298 39.0 35.0 40.5 30.0 41.0 42-43 35.94840013527444 39.0 35.0 40.5 30.0 41.0 44-45 35.884745260301436 39.0 35.0 41.0 29.0 41.0 46-47 35.603057630171094 39.0 35.0 41.0 27.0 41.0 48-49 35.34113453287536 39.0 35.0 41.0 26.0 41.0 50-51 34.078593125450745 38.0 34.0 40.0 24.0 40.5 52-53 34.27271856804671 38.0 33.5 39.5 23.5 40.5 54-55 34.849844352903304 38.5 35.0 40.0 24.5 41.0 56-57 34.7618899917287 38.5 34.5 40.0 24.0 41.0 58-59 34.60415775362939 38.0 34.0 40.0 23.5 41.0 60-61 34.188292241684906 38.0 34.0 40.0 22.5 41.0 62-63 33.861933996667034 37.5 34.0 40.0 22.0 41.0 64-65 33.37126925725369 37.0 33.0 39.5 21.5 41.0 66-67 32.8457066663407 36.0 33.0 39.0 19.5 40.5 68-69 32.41283202337151 36.0 32.0 39.0 18.5 40.0 70-71 31.783783976498103 35.0 31.5 37.5 15.0 39.5 72-73 31.23276422317023 35.0 31.0 37.0 10.0 39.0 74-75 30.784279541370754 35.0 31.0 37.0 7.0 39.0 76-77 30.326992343955638 34.5 30.5 36.0 2.0 37.5 78-79 29.841694271616404 34.0 30.0 35.5 2.0 37.0 80-81 29.351872960187755 34.0 29.0 35.0 2.0 36.5 82-83 28.925822647874927 34.0 29.0 35.0 2.0 36.0 84-85 28.5199020482669 34.0 29.0 35.0 2.0 36.0 86-87 28.17481522000432 34.0 29.0 35.0 2.0 35.0 88-89 27.632358603576627 34.0 26.5 35.0 2.0 35.0 90-91 27.064191185158926 33.5 25.5 35.0 2.0 35.0 92-93 26.58872037306409 33.0 25.0 35.0 2.0 35.0 94-95 26.021148345536556 33.0 24.0 35.0 2.0 35.0 96-97 25.289823959874013 33.0 19.5 35.0 2.0 35.0 98-99 24.579516516112733 32.0 7.5 35.0 2.0 35.0 100-101 22.049463282360946 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06088579585579268 1101 2 0.22350267847591354 1101 3 0.26221351599638254 1101 4 0.19750860162940853 1101 5 0.022865397718170755 1101 6 0.11725764521272453 1101 7 0.11561646702588035 1101 8 0.18154308834873234 1101 9 0.20108407636308812 1101 10-11 0.1974459857994404 1101 12-13 0.2672619141918773 1101 14-15 -0.11383887606753262 1101 16-17 0.32360865253863125 1101 18-19 0.37114299122541894 1101 20-21 0.5159571080259013 1101 22-23 0.5876514098127714 1101 24-25 0.49472624002749654 1101 26-27 0.49952197278044963 1101 28-29 0.3945750903784955 1101 30-31 0.3678508666661173 1101 32-33 0.4526411230127323 1101 34-35 0.3195932656471214 1101 36-37 0.37217352551404304 1101 38-39 0.4031322533367927 1101 40-41 0.6243938951139896 1101 42-43 0.41836318159222685 1101 44-45 0.6528268771093053 1101 46-47 0.6718140054513952 1101 48-49 0.6912347737086009 1101 50-51 0.5368408972384415 1101 52-53 0.19152038831912677 1101 54-55 0.31638960955005047 1101 56-57 0.4289687836611762 1101 58-59 0.3103236700568033 1101 60-61 0.4236083017336796 1101 62-63 0.367845656389548 1101 64-65 0.1492337301450064 1101 66-67 0.025650803155301105 1101 68-69 -0.07569471925985027 1101 70-71 0.06320255445082168 1101 72-73 0.021991816387071594 1101 74-75 -0.12106022971656927 1101 76-77 -0.0872386983372202 1101 78-79 -0.24116101488164432 1101 80-81 -0.1265799029883432 1101 82-83 -0.1130548000331757 1101 84-85 -0.3996834621872516 1101 86-87 -0.46004282497293403 1101 88-89 -0.37453247159909253 1101 90-91 -0.3085057478858495 1101 92-93 -0.3209413374956327 1101 94-95 -0.09810511763912189 1101 96-97 -0.19268646800489364 1101 98-99 -0.44906802452000605 1101 100-101 -0.06837653164441448 1102 1 0.29064855837168935 1102 2 0.2541474653925526 1102 3 0.04117065754098803 1102 4 0.29451677873062465 1102 5 0.22445923128442047 1102 6 0.2598757682996151 1102 7 0.2419178640480837 1102 8 0.1429146266205663 1102 9 0.3307776889396763 1102 10-11 0.23852914948169968 1102 12-13 0.2501998920155444 1102 14-15 0.4004988561045906 1102 16-17 0.18442687040445804 1102 18-19 0.32663980694325545 1102 20-21 0.40724240229999253 1102 22-23 0.26913494197232524 1102 24-25 0.18188744159232328 1102 26-27 0.4452362481326091 1102 28-29 0.37673694475742536 1102 30-31 0.3712189039200524 1102 32-33 0.3186093188763053 1102 34-35 0.3332376850535965 1102 36-37 0.34013606849097755 1102 38-39 0.3488229548459998 1102 40-41 0.29078956332967465 1102 42-43 0.28743345340468096 1102 44-45 0.3165677679346146 1102 46-47 0.42096232481772944 1102 48-49 0.4785370556566022 1102 50-51 0.403454049266756 1102 52-53 0.4773429582790456 1102 54-55 0.4890619645648755 1102 56-57 0.45829947994786835 1102 58-59 0.45310378775676696 1102 60-61 0.33580117687210276 1102 62-63 0.2549577760044386 1102 64-65 0.35421615252057137 1102 66-67 0.3897560609685158 1102 68-69 0.3340881837803309 1102 70-71 0.26603189917052106 1102 72-73 0.2273302642368975 1102 74-75 0.12964094099674028 1102 76-77 0.1000657596578165 1102 78-79 -0.07917546866966063 1102 80-81 -0.03966918861627633 1102 82-83 -0.12027444055020453 1102 84-85 -0.10000793497940919 1102 86-87 -0.3143999964986328 1102 88-89 -0.19285067737577322 1102 90-91 -0.5598137939406733 1102 92-93 -0.7627864803168514 1102 94-95 -0.5943393473426504 1102 96-97 -0.3738647491211573 1102 98-99 -0.5848225325641252 1102 100-101 -0.10594296152492788 1103 1 0.11643828217634322 1103 2 0.27339602923182227 1103 3 0.3591490316358694 1103 4 0.34922435737382074 1103 5 0.32270830626404035 1103 6 0.4158130763975407 1103 7 0.2772104889033926 1103 8 0.1972774548666365 1103 9 0.3389822695711686 1103 10-11 0.4005869741785162 1103 12-13 0.5293878611843326 1103 14-15 0.25931818175845933 1103 16-17 0.23431333021290612 1103 18-19 0.3426509490845575 1103 20-21 0.6101585357806485 1103 22-23 0.5047664516798065 1103 24-25 0.5836156226628901 1103 26-27 0.40207062549304595 1103 28-29 0.48317743281864267 1103 30-31 0.39097501695508186 1103 32-33 0.4304939902920708 1103 34-35 0.487303405492149 1103 36-37 0.5416845723580508 1103 38-39 0.5313467432219312 1103 40-41 0.2997039946052382 1103 42-43 0.33839486410415276 1103 44-45 0.47071548629747895 1103 46-47 0.37020261704157775 1103 48-49 0.21271474815121394 1103 50-51 0.2957871834907735 1103 52-53 0.5046644272509084 1103 54-55 0.581289878094772 1103 56-57 0.5812706298460597 1103 58-59 0.5286639772258397 1103 60-61 0.527777470494101 1103 62-63 0.2189660739549737 1103 64-65 0.14837981558930835 1103 66-67 0.1664356118832444 1103 68-69 -0.12919889155121922 1103 70-71 -0.07656560354379138 1103 72-73 0.004205532824990144 1103 74-75 -0.23756371983710167 1103 76-77 -0.08570861796596674 1103 78-79 -0.1476970041975072 1103 80-81 -0.4155363017027902 1103 82-83 -0.3488577199165306 1103 84-85 -0.5381641428208077 1103 86-87 -0.17591866987995175 1103 88-89 -0.35213733134573033 1103 90-91 -0.5067880705334353 1103 92-93 -0.33791273916454756 1103 94-95 -0.6354095339747161 1103 96-97 -0.8460782262281761 1103 98-99 -0.9354552333363415 1103 100-101 -0.7942969818771708 1104 1 0.04480259790085839 1104 2 0.061971853667756704 1104 3 -0.06711456806994676 1104 4 0.010032689227770675 1104 5 -0.04995110722619245 1104 6 0.051319826585633166 1104 7 -0.04913172914839947 1104 8 -0.005997464190286905 1104 9 0.03986059711379397 1104 10-11 -0.0312855795651501 1104 12-13 0.07557402604615504 1104 14-15 0.17028501834219156 1104 16-17 0.045451774584087445 1104 18-19 0.061328301083413805 1104 20-21 0.07989047258164561 1104 22-23 0.07854934669472868 1104 24-25 0.2130653787769461 1104 26-27 0.09947452935886503 1104 28-29 0.007071492484051589 1104 30-31 0.15251627467954876 1104 32-33 0.1658249058999317 1104 34-35 0.0626163994985589 1104 36-37 0.10415234306299226 1104 38-39 0.011983805431860617 1104 40-41 0.13438567483991193 1104 42-43 0.1798563199806651 1104 44-45 0.16340651619781 1104 46-47 0.22630990994986178 1104 48-49 0.304685532559418 1104 50-51 0.29582099783313254 1104 52-53 0.36227645839392153 1104 54-55 0.33262343122132876 1104 56-57 0.3744832459771885 1104 58-59 0.4441872964416902 1104 60-61 0.36803851721706593 1104 62-63 0.020560420196929385 1104 64-65 0.13033563715102048 1104 66-67 0.1542962629784057 1104 68-69 0.21276555266779695 1104 70-71 0.02669917121042431 1104 72-73 0.07964533061522161 1104 74-75 0.19568254156467546 1104 76-77 0.2327421779391159 1104 78-79 0.2053712246917776 1104 80-81 0.046591634861503195 1104 82-83 -0.09999261378176527 1104 84-85 -0.24760595256440965 1104 86-87 -0.07979294815508098 1104 88-89 -0.09253194472098869 1104 90-91 -0.09303577749834702 1104 92-93 0.06466391372277158 1104 94-95 -0.007168640599786613 1104 96-97 0.09681780312267207 1104 98-99 -0.12833646891241202 1104 100-101 0.6252798316282764 1105 1 0.1309201515480325 1105 2 0.08905139983462362 1105 3 0.30762860110059975 1105 4 0.24580513188919895 1105 5 0.351303968447489 1105 6 0.3637828255339315 1105 7 0.1829771740881938 1105 8 0.2595429775239211 1105 9 0.30169222752813596 1105 10-11 0.2626559988213586 1105 12-13 0.37802323448482866 1105 14-15 0.3679350597833704 1105 16-17 0.3621999306257848 1105 18-19 0.5021875612713202 1105 20-21 0.43583884871430456 1105 22-23 0.3160752039002688 1105 24-25 0.34192306489659074 1105 26-27 0.3040650974860739 1105 28-29 0.5956493984160574 1105 30-31 0.6552865651856905 1105 32-33 0.5294277113631907 1105 34-35 0.4278333214289489 1105 36-37 0.33162087910984894 1105 38-39 0.20470965985144574 1105 40-41 0.4005912648717427 1105 42-43 0.31293299660714524 1105 44-45 0.0070287537428228575 1105 46-47 0.44882639935972435 1105 48-49 0.39500466445380766 1105 50-51 0.4085357780555938 1105 52-53 0.28976658564271673 1105 54-55 0.20458238007803686 1105 56-57 0.07419315773186241 1105 58-59 0.034762388552543655 1105 60-61 0.23592647977374526 1105 62-63 0.2610393966346578 1105 64-65 0.4946192619655818 1105 66-67 0.20653652430159752 1105 68-69 0.326074090943429 1105 70-71 0.07080790728932129 1105 72-73 0.4105008994040915 1105 74-75 0.46345523496264107 1105 76-77 0.5073387358836321 1105 78-79 0.46941421207833756 1105 80-81 0.3161456024189846 1105 82-83 0.21921415331881278 1105 84-85 0.4607996416463642 1105 86-87 0.597542209983061 1105 88-89 0.2752080136288839 1105 90-91 0.3157727364001168 1105 92-93 0.5489180073450122 1105 94-95 0.31010884813447603 1105 96-97 -0.05063791750110269 1105 98-99 0.3006075820149654 1105 100-101 0.4668230453742126 1106 1 0.14640306626180788 1106 2 0.19384649657780173 1106 3 0.23122218627332458 1106 4 0.27243120514428654 1106 5 0.24443914183743942 1106 6 0.2302986022167559 1106 7 0.23877976191818107 1106 8 0.17641995545125155 1106 9 0.23757733749088317 1106 10-11 0.08757688172806155 1106 12-13 0.19809026702633048 1106 14-15 0.26234545383323393 1106 16-17 0.25990853936672664 1106 18-19 0.1627418482827636 1106 20-21 0.31290357117021017 1106 22-23 0.2103587435389329 1106 24-25 0.19240177338514997 1106 26-27 0.20796286618374182 1106 28-29 0.1958777363562092 1106 30-31 0.10186416670259746 1106 32-33 0.3302061968550589 1106 34-35 0.3610377973867003 1106 36-37 0.21193708042112291 1106 38-39 0.3172595670474223 1106 40-41 0.35915660824660023 1106 42-43 0.35310578566828354 1106 44-45 0.4067937392547094 1106 46-47 0.5729534407142651 1106 48-49 0.399798425887397 1106 50-51 0.3756504410902153 1106 52-53 0.21029897357685456 1106 54-55 0.37914740297859595 1106 56-57 0.17808024395279887 1106 58-59 0.5744678192566468 1106 60-61 0.6687470542239282 1106 62-63 0.6029520940935669 1106 64-65 0.40777072613254717 1106 66-67 0.4765695064485129 1106 68-69 0.41638441689249106 1106 70-71 0.34042694346285884 1106 72-73 0.42261215739925717 1106 74-75 0.3960020709465617 1106 76-77 0.40977767885256 1106 78-79 0.5832693844000367 1106 80-81 0.6649885566579243 1106 82-83 0.669634108185349 1106 84-85 0.37726267360032395 1106 86-87 0.5279759185383881 1106 88-89 0.5546649432180075 1106 90-91 0.3855793835712653 1106 92-93 0.47197746868112134 1106 94-95 0.7402320972097769 1106 96-97 0.6371733765474197 1106 98-99 0.5051569817933022 1106 100-101 0.8471993356171019 1107 1 0.048233859605588236 1107 2 0.059843984378073145 1107 3 0.12734717376790528 1107 4 0.05356414052739922 1107 5 -0.029615170586836825 1107 6 0.05389994057448888 1107 7 0.08903598405888857 1107 8 0.04927263605728882 1107 9 0.1976255806184568 1107 10-11 0.1168378948337434 1107 12-13 0.15206309969826037 1107 14-15 0.10741720714855774 1107 16-17 0.21004971165249486 1107 18-19 0.22838807275781647 1107 20-21 0.25126686192953684 1107 22-23 0.1079237995076312 1107 24-25 0.1448767347572968 1107 26-27 0.29939915524233385 1107 28-29 0.2646358648785707 1107 30-31 0.23738289148333536 1107 32-33 0.10819443620322033 1107 34-35 0.051081260665235106 1107 36-37 -0.02058312277803509 1107 38-39 0.13354718056883996 1107 40-41 0.13034968080026488 1107 42-43 0.13458720539461666 1107 44-45 0.24947390065798913 1107 46-47 0.13247145407560623 1107 48-49 0.047053903260923846 1107 50-51 0.17707661465431102 1107 52-53 0.06711025846697538 1107 54-55 0.11597502828289663 1107 56-57 0.3873814967178859 1107 58-59 0.17696112191001845 1107 60-61 0.0846422754864804 1107 62-63 -0.1433307395788006 1107 64-65 -0.018922507602070482 1107 66-67 -0.11871400529547316 1107 68-69 -0.15172454143388592 1107 70-71 0.017493672378723346 1107 72-73 -0.12164646578189675 1107 74-75 0.03749894181282798 1107 76-77 0.09541923463784485 1107 78-79 0.21511651815800192 1107 80-81 0.06617962834454261 1107 82-83 -0.06632647473391629 1107 84-85 0.1728146350058175 1107 86-87 0.22075612329378203 1107 88-89 0.09460169358761306 1107 90-91 -0.03442428468498093 1107 92-93 0.09581835528753757 1107 94-95 0.009657181412933369 1107 96-97 0.30347043860765055 1107 98-99 0.1681742892932192 1107 100-101 0.22534708853225638 1108 1 0.15917554926209831 1108 2 0.2086412333373069 1108 3 0.06917172857587062 1108 4 0.046365878725175946 1108 5 0.0941175317467966 1108 6 0.20598671777667477 1108 7 0.1555187320036211 1108 8 0.15252843972497487 1108 9 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-2.0204137289618274 2316 88-89 -1.9872180367430197 2316 90-91 -2.248666646443567 2316 92-93 -2.653544228087629 2316 94-95 -2.7898972545047336 2316 96-97 -2.779555093917633 2316 98-99 -3.1299506658814167 2316 100-101 -3.5292739578709558 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3720.0 3 751.0 4 641.0 5 706.0 6 797.0 7 1001.0 8 1299.0 9 1675.0 10 2024.0 11 2998.0 12 4134.0 13 6493.0 14 6283.0 15 6087.0 16 7469.0 17 9995.0 18 8372.0 19 6245.0 20 6566.0 21 6951.0 22 7914.0 23 8688.0 24 10158.0 25 11401.0 26 13470.0 27 15527.0 28 18678.0 29 22044.0 30 26272.0 31 31655.0 32 38614.0 33 48485.0 34 64716.0 35 95296.0 36 133521.0 37 192910.0 38 141800.0 39 16351.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.61150679390708 17.807564356476096 13.602216050637411 18.97871279897941 2 14.248488617480042 24.976297036364933 35.77577506142381 24.999439284731213 3 27.01922086819641 25.02811305577447 30.416440249943673 17.53622582608545 4 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28.387384925377397 25.035638972618663 24-25 24.1774296192648 26.48996862901877 28.107624086712818 21.22497766500361 26-27 23.70999383471215 22.84343687515267 33.32707795442991 20.119491335705277 28-29 19.468357799657444 29.891934807006777 30.820735585741083 19.8189718075947 30-31 20.24520796176363 27.50696740995904 29.101543180606075 23.146281447671257 32-33 20.642025964915142 28.04458615612394 29.686563176806956 21.626824702153954 34-35 23.829967354108238 25.20266933586621 28.946313713421624 22.021049596603927 36-37 22.770237909246678 24.484188473093383 32.517678636830475 20.22789498082947 38-39 22.604793945955688 25.81854665651923 31.907929441867182 19.668729955657895 40-41 20.70071879095675 27.719681766055043 31.130252703145477 20.449346739842724 42-43 19.027430335959526 29.43395646685597 27.740562740524332 23.79805045666017 44-45 21.170685033808372 26.499864008437115 27.819468767271054 24.50998219048346 46-47 23.024458548097645 28.407889669187842 27.314333008284645 21.253318774429868 48-49 22.608494308969743 26.74387213405823 28.611187808864987 22.036445748107045 50-51 19.479090580337434 28.291616255471812 31.99902882683716 20.230264337353592 52-53 21.077912099170167 29.907898780862617 27.867943858211248 21.146245261755965 54-55 18.061836914689724 26.519161289595612 30.528480976999894 24.89052081871477 56-57 22.127864608401964 27.01103753663647 27.74407415979338 23.117023695168186 58-59 24.57310797872068 25.85135197407159 27.351554219766825 22.223985827440902 60-61 21.00521630015757 26.260000296816845 31.875928512174863 20.858854890850722 62-63 21.50078586840123 26.135079229567292 30.457329829659376 21.906805072372105 64-65 20.15644739713704 29.763633178036912 29.284782624287516 20.795136800538533 66-67 20.66524159302192 26.595368578970668 27.977847141381517 24.761542686625894 68-69 18.94369329209681 28.20536074783211 29.078152636616473 23.772793323454604 70-71 22.60252951830532 27.761271266116903 26.63049409377714 23.005705121800634 72-73 21.327118623227907 24.848559987868406 30.189535679594737 23.63478570930895 74-75 19.522163808181084 30.446073343490614 27.90801684045726 22.12374600787104 76-77 21.691137865947514 25.6267111271606 28.008267193226434 24.673883813665455 78-79 21.141184477424833 26.270174825873145 29.924777607703625 22.663863088998397 80-81 22.706666427699073 27.090270861230664 28.287805739830862 21.915256971239398 82-83 21.19157780015303 26.00337813960587 29.950720953172098 22.854323107068996 84-85 20.76911729906976 26.667721781566627 29.138995510127568 23.424165409236046 86-87 21.528114585803376 27.12455372758779 28.569065377147883 22.77826630946095 88-89 21.614076855582947 26.40701199757621 27.34154548122712 24.637365665613725 90-91 21.83496333833494 24.418723191493612 28.742304841245485 25.004008628925963 92-93 22.834540379697028 27.286266722408026 28.531532805429865 21.34766009246508 94-95 22.10716523480147 26.546519437896638 28.118796054886484 23.227519272415407 96-97 19.337902783320672 25.904621483450647 30.15852360896724 24.598952124261444 98-99 21.99033538231699 27.07722164784191 26.73436954046956 24.198073429371544 100-101 22.23071355727579 24.670481279423374 28.144302385319598 24.95450277798124 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6752.0 1 4193.0 2 1782.5 3 1819.5 4 1396.0 5 1286.0 6 2127.5 7 3445.0 8 4542.0 9 4601.5 10 3524.0 11 2660.0 12 2486.0 13 2476.0 14 2511.0 15 2590.5 16 2914.5 17 2994.0 18 2688.5 19 2535.0 20 2550.5 21 2706.5 22 2956.5 23 3386.0 24 3681.5 25 4028.0 26 4739.0 27 5510.0 28 6109.0 29 6684.5 30 7459.0 31 8464.0 32 9608.0 33 10374.5 34 11191.0 35 11695.5 36 11959.5 37 13129.5 38 14502.0 39 15123.5 40 15347.0 41 16273.5 42 17536.5 43 18476.5 44 20123.5 45 22673.0 46 33660.0 47 65403.5 48 133880.0 49 135458.0 50 70812.5 51 40851.0 52 27468.5 53 23373.0 54 21101.5 55 18199.5 56 15396.0 57 13176.0 58 12008.0 59 10715.0 60 9134.0 61 7472.0 62 5919.5 63 4503.0 64 3148.5 65 2282.0 66 1577.0 67 1169.5 68 826.0 69 564.0 70 426.0 71 288.0 72 176.0 73 117.5 74 97.0 75 75.5 76 49.0 77 32.0 78 23.5 79 18.0 80 14.5 81 9.5 82 9.5 83 11.5 84 10.5 85 6.0 86 1.5 87 1.0 88 2.0 89 2.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27187310279635085 2 0.0833241656378475 3 0.08627820084994724 4 0.10115024019362172 5 0.10288191600761123 6 0.0777216850631756 7 0.08841732979664013 8 0.08525956801818871 9 0.08994527904427793 10-11 0.08296764414673202 12-13 0.08724590204011784 14-15 0.09463099007036715 16-17 0.08469931996072153 18-19 0.0943254002208396 20-21 0.09585334946847739 22-23 0.10593781450288679 24-25 0.1206061272802096 26-27 0.12412041054977652 28-29 0.11637880102841171 30-31 0.10909557628133823 32-33 0.1018632831758527 34-35 0.37047676091057624 36-37 0.5704853174263631 38-39 0.5536269440607594 40-41 0.5162431191352215 42-43 0.5424729145530036 44-45 0.5647809735685153 46-47 0.5545437136093421 48-49 0.5759859347178591 50-51 0.5672766240063237 52-53 0.5712492920501819 54-55 0.5597387410513106 56-57 0.5319300647443028 58-59 0.526938763868686 60-61 0.4761599172055235 62-63 0.5500107975080166 64-65 0.5831672961817567 66-67 0.59610393314509 68-69 0.5983449253749588 70-71 0.5939138725568092 72-73 0.5851026985620978 74-75 0.5831672961817567 76-77 0.5664107860993289 78-79 0.5435934106679379 80-81 0.5383983832259694 82-83 0.553219490928056 84-85 0.5613685535821242 86-87 0.5527611061537646 88-89 0.5655958798339221 90-91 0.579449286345838 92-93 0.5879548704910218 94-95 0.5873946224335546 96-97 0.5840331340887515 98-99 0.588464186906901 100-101 0.5652902899843946 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 981708.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.29160961156436 #Duplication Level Percentage of deduplicated Percentage of total 1 69.42565151096576 21.030147325986885 2 14.040256152440545 8.506039164321875 3 6.052747120437652 5.500423585494532 4 3.1616187716879187 3.8308208629025633 5 1.9146248825692764 2.89985347476879 6 1.2281552091884187 2.232167888348685 7 0.8263605886509696 1.7522254644858386 8 0.5701775628174399 1.3817276913711285 9 0.43708421106923395 1.191598586017905 >10 2.097342248946771 11.303674348199701 >50 0.13545844982360558 2.906573861370836 >100 0.08491423607751532 4.722824600299073 >500 0.006065302576964538 1.338048577594892 >1k 0.012130605153929075 9.089115240605107 >5k 0.005391380068412922 11.246589707354914 >10k+ 0.002021767525654846 11.068169620877239 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 36468 3.7147502108569963 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 19375 1.973601111532146 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14504 1.4774250591825675 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 13555 1.3807568034486832 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12470 1.2702351412028832 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 12064 1.228878648233487 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9166 0.9336788535898657 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8977 0.9144266930696296 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8738 0.8900813683906008 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8331 0.8486230121380288 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8266 0.8420018987315984 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7680 0.7823100147905487 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 7657 0.7799671592775042 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7381 0.7518528931209687 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6498 0.6619076140766909 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5893 0.6002803277552999 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5683 0.5788890382883709 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5497 0.5599424676176623 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5267 0.5365139124872161 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5089 0.5183822480819144 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5043 0.5136965370558252 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 5018 0.5111499549764288 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4910 0.5001487203934367 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4796 0.48853630611138954 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4709 0.47967420047509035 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4632 0.4718307276705497 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4552 0.46368166501648145 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4088 0.4164171016228858 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3849 0.392071776943857 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3517 0.35825316692947395 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3389 0.3452146666829648 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3310 0.3371674673120724 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3251 0.33115753360469713 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3022 0.30783084175742687 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3022 0.30783084175742687 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2847 0.29000476720165264 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2691 0.27411409502621964 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2495 0.2541488915237525 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2252 0.2293961137120203 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2099 0.2138110313861148 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2054 0.20922718364320145 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1982 0.20189302725454003 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1830 0.18640980821181044 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1628 0.1658334250102882 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1584 0.16135144055055067 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1571 0.16002721786926458 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1528 0.15564709669270294 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1476 0.15035020596755858 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1447 0.14739617075545886 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1343 0.13680238930517016 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1322 0.13466326035847725 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1228 0.1250881117399471 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1180 0.12019867414750618 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1179 0.12009681086433033 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1105 0.11255892790931724 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1098 0.11184588492708625 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1057 0.10766949031687631 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1004 0.1022707363085561 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.0186328317585269E-4 0.0 9 0.0 0.0 0.0 2.0372656635170538E-4 0.0 10-11 0.0 0.0 0.0 3.055898495275581E-4 0.0 12-13 0.0 0.0 0.0 4.0745313270341076E-4 0.0 14-15 0.0 0.0 0.0 4.5838477429133715E-4 0.0 16-17 0.0 0.0 0.0 7.639746238188951E-4 0.0 18-19 0.0 0.0 0.0 9.167695485826743E-4 0.0 20-21 0.0 0.0 0.0 0.001629812530813643 0.0 22-23 0.0 0.0 0.0 0.0034633516279789917 0.0 24-25 0.0 0.0 0.0 0.006162728632139089 0.0 26-27 0.0 0.0 0.0 0.010797508016640386 0.0 28-29 0.0 0.0 0.0 0.03010060017846447 0.0 30-31 0.0 0.0 0.0 0.07583721432442234 0.0 32-33 0.0 0.0 0.0 0.17953403659744038 0.0 34-35 0.0 0.0 0.0 0.3480668386118887 0.0 36-37 0.0 0.0 0.0 0.5780232003813761 0.0 38-39 0.0 0.0 0.0 0.889317393766782 0.0 40-41 0.0 0.0 0.0 1.2089643763726077 0.0 42-43 0.0 0.0 0.0 1.5394597986366618 0.0 44-45 0.0 0.0 0.0 1.90866326850754 0.0 46-47 0.0 0.0 0.0 2.3776927558907537 0.0 48-49 0.0 0.0 0.0 2.833989332876986 0.0 50-51 0.0 0.0 0.0 3.322474707346787 0.0 52-53 0.0 0.0 0.0 3.748772547437731 0.0 54-55 0.0 0.0 0.0 4.209652972166877 0.0 56-57 0.0 0.0 0.0 4.838200361003476 0.0 58-59 0.0 0.0 0.0 5.44377757948392 0.0 60-61 0.0 0.0 0.0 6.23072237365897 0.0 62-63 0.0 0.0 0.0 6.940556662469899 0.0 64-65 0.0 0.0 0.0 7.542466802756013 0.0 66-67 0.0 0.0 0.0 8.253981835739344 0.0 68-69 0.0 0.0 0.0 9.095270691488711 0.0 70-71 0.0 0.0 0.0 9.972466354557568 0.0 72-73 0.0 0.0 0.0 10.818950237748902 0.0 74-75 0.0 0.0 0.0 11.74300301107865 0.0 76-77 0.0 0.0 0.0 12.52551675243555 0.0 78-79 0.0 0.0 0.0 13.664144531775232 0.0 80-81 0.0 1.0186328317585269E-4 0.0 14.859662954768627 0.0 82-83 0.0 1.0186328317585269E-4 0.0 15.863678405391418 0.0 84-85 0.0 1.0186328317585269E-4 0.0 17.0795185533784 0.0 86-87 0.0 1.0186328317585269E-4 0.0 18.303049379245152 0.0 88-89 0.0 1.0186328317585269E-4 0.0 19.47294918651982 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATAC 55 0.0026190768 34.438194 3 GAATTCG 60 0.004013271 31.565126 7 CTTTGCG 710 0.0 29.345222 2 TATAGGG 85 6.5240555E-4 27.85442 2 TTTGCGT 765 0.0 27.235437 3 GTATACT 105 7.052538E-5 27.058582 4 GTCTATA 125 7.4835953E-6 26.556673 1 GCTTTGC 840 0.0 25.404978 1 GTAAGCG 210 0.0 24.9142 94-95 CGGGAGT 175 1.5310252E-7 24.352722 4 GAGTACT 4335 0.0 22.17261 12-13 CATGGGG 1160 0.0 22.043413 4 TTGCGTT 955 0.0 21.816866 4 ATGGGAG 715 0.0 21.192736 5 GTATAGT 135 3.8374012E-4 21.076723 1 AGTACTT 4355 0.0 21.037914 12-13 TACATGG 5380 0.0 20.947767 2 GTACATG 5505 0.0 20.76086 1 AGTCCGT 80 5.11559E-5 20.715145 12-13 CCGTATC 165 2.7284841E-11 20.178442 94-95 >>END_MODULE