##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939054_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 981708 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.965535576770282 33.0 31.0 34.0 30.0 34.0 2 32.15902182726432 33.0 31.0 34.0 30.0 34.0 3 32.26867867023596 34.0 31.0 34.0 30.0 34.0 4 35.81535242658713 37.0 35.0 37.0 35.0 37.0 5 35.74610169215286 37.0 35.0 37.0 33.0 37.0 6 35.85259873608038 37.0 35.0 37.0 35.0 37.0 7 35.79999857391404 37.0 35.0 37.0 33.0 37.0 8 35.806059439263 37.0 35.0 37.0 33.0 37.0 9 37.39343368903992 39.0 37.0 39.0 34.0 39.0 10-11 37.30174960375183 39.0 37.0 39.0 33.5 39.0 12-13 37.25875667713821 39.0 37.0 39.0 33.0 39.0 14-15 38.25019608682011 40.0 37.5 41.0 33.0 41.0 16-17 38.32089990098889 40.0 37.5 41.0 33.5 41.0 18-19 38.52595883908454 40.0 38.0 41.0 34.0 41.0 20-21 38.51321523304282 40.0 38.0 41.0 34.0 41.0 22-23 38.46834751270235 40.0 38.0 41.0 34.0 41.0 24-25 38.33969011151992 40.0 38.0 41.0 33.5 41.0 26-27 38.04875380459363 40.0 37.0 41.0 33.5 41.0 28-29 37.87046657458225 40.0 37.0 41.0 33.0 41.0 30-31 37.7905996487754 40.0 37.0 41.0 33.0 41.0 32-33 37.59504506431648 40.0 36.0 41.0 33.0 41.0 34-35 37.484549377207884 40.0 36.0 41.0 32.5 41.0 36-37 37.29531693741927 39.5 35.5 41.0 31.5 41.0 38-39 37.09243532700151 39.0 35.5 41.0 31.0 41.0 40-41 37.02202284182262 39.0 35.0 41.0 31.0 41.0 42-43 36.870575058978844 39.0 35.0 41.0 31.0 41.0 44-45 36.64456793669808 39.0 35.0 41.0 30.5 41.0 46-47 36.29827810306119 39.0 35.0 41.0 30.0 41.0 48-49 35.95465912470918 39.0 35.0 41.0 29.0 41.0 50-51 35.66364692963692 39.0 35.0 40.0 28.0 41.0 52-53 35.45452568380822 38.5 35.0 40.0 28.0 41.0 54-55 35.277316167332856 38.0 35.0 40.0 27.0 41.0 56-57 34.97630048853631 38.0 34.0 40.0 26.5 41.0 58-59 34.71234878395613 38.0 34.0 40.0 26.0 41.0 60-61 34.10568723082628 37.5 33.0 40.0 25.0 41.0 62-63 33.837826013437805 37.0 33.0 40.0 24.5 41.0 64-65 33.53735071935851 36.5 33.0 39.0 23.5 40.5 66-67 32.980779926413966 36.0 32.0 39.0 22.0 40.0 68-69 32.5210021717252 35.5 32.0 38.0 22.0 40.0 70-71 31.89143615005684 35.0 31.5 37.0 20.5 39.0 72-73 31.28403761607321 35.0 31.0 37.0 18.5 39.0 74-75 30.858401378006494 34.0 31.0 36.0 17.0 38.0 76-77 28.85147925859828 32.5 28.0 34.5 11.0 36.0 78-79 29.835513207593298 34.0 29.5 35.0 9.5 36.5 80-81 29.82750318832076 34.0 30.0 35.0 7.0 36.0 82-83 29.49828564094415 34.0 29.5 35.0 4.5 36.0 84-85 29.117207968153462 34.0 29.0 35.0 2.0 35.5 86-87 28.65633569248697 34.0 29.0 35.0 2.0 35.0 88-89 28.023076108170656 33.0 27.5 35.0 2.0 35.0 90-91 27.461009281782363 33.0 26.5 35.0 2.0 35.0 92-93 27.150348168701896 33.0 26.0 35.0 2.0 35.0 94-95 26.753740419758216 33.0 25.0 34.5 2.0 35.0 96-97 26.036181838184064 32.0 24.5 34.0 2.0 35.0 98-99 25.157611020792334 32.0 20.5 34.0 2.0 35.0 100-101 20.97592308507214 27.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19990391052172285 1101 2 0.21461528906903027 1101 3 0.13755472493155452 1101 4 0.16413984072080723 1101 5 0.1274383257129017 1101 6 0.11277495521355263 1101 7 0.18243463986051012 1101 8 0.13703952455607293 1101 9 0.2554426170551167 1101 10-11 0.17001827030772887 1101 12-13 0.184396542606315 1101 14-15 0.40576992112853816 1101 16-17 0.31502890359848124 1101 18-19 0.24322612645286767 1101 20-21 0.30311705108599796 1101 22-23 0.3751896615355079 1101 24-25 0.38679967278165606 1101 26-27 0.30764143121749754 1101 28-29 0.26795653416353815 1101 30-31 0.38171273005055895 1101 32-33 0.42970399999708064 1101 34-35 0.47668113399097223 1101 36-37 0.6450317308683466 1101 38-39 0.5097337517537923 1101 40-41 0.4955731540839281 1101 42-43 0.41896307420560674 1101 44-45 0.5779439391072358 1101 46-47 0.5951421909413455 1101 48-49 0.09822616709112708 1101 50-51 0.4952754476400898 1101 52-53 0.3770520781349731 1101 54-55 0.40861983424253623 1101 56-57 0.3711308801155653 1101 58-59 0.34174864600320376 1101 60-61 0.4749890088851316 1101 62-63 0.4515783405148426 1101 64-65 0.18480485006336522 1101 66-67 0.21426880353632072 1101 68-69 0.21158125647592385 1101 70-71 0.3675865284490243 1101 72-73 0.06995140855921278 1101 74-75 0.13320165609842505 1101 76-77 -0.02358137793379811 1101 78-79 0.3300129869629451 1101 80-81 0.19018861475607807 1101 82-83 0.14246602861058477 1101 84-85 0.18631434155296844 1101 86-87 0.4740557261600813 1101 88-89 0.5927836895879359 1101 90-91 0.3962464632035463 1101 92-93 0.2687219655296893 1101 94-95 0.06602365947337319 1101 96-97 0.44851076355119446 1101 98-99 0.5747283242843757 1101 100-101 0.9629615459692218 1102 1 0.17185949981944404 1102 2 0.1683005456249731 1102 3 0.21294991925255147 1102 4 0.10816828407347145 1102 5 0.09376520666378951 1102 6 0.05465878331551011 1102 7 0.18351420097599913 1102 8 0.1569358480037053 1102 9 0.31027779358088736 1102 10-11 0.2320198950895147 1102 12-13 0.09556848900785297 1102 14-15 0.02424238446214133 1102 16-17 0.27768128733712416 1102 18-19 0.1209316782742178 1102 20-21 0.3195341170975965 1102 22-23 0.28283338944259384 1102 24-25 0.28222066534925005 1102 26-27 0.16969636915180075 1102 28-29 0.24379757634499555 1102 30-31 0.20591412078641014 1102 32-33 0.1117055522439756 1102 34-35 0.27576489729508324 1102 36-37 0.23675634486177444 1102 38-39 0.36048835651111233 1102 40-41 0.35814188090297705 1102 42-43 0.35249511287154434 1102 44-45 0.35476492780630053 1102 46-47 0.4075941365691804 1102 48-49 0.24766257090742982 1102 50-51 0.4972236141995623 1102 52-53 0.4876242817936358 1102 54-55 0.33308621915141856 1102 56-57 0.42351075022974527 1102 58-59 0.2430498012423996 1102 60-61 0.05733909133314086 1102 62-63 0.31810747294906605 1102 64-65 0.2665819067904991 1102 66-67 0.538239895960281 1102 68-69 0.581501395549509 1102 70-71 0.2615942993547904 1102 72-73 0.3412506778909723 1102 74-75 0.6576241573919646 1102 76-77 0.4700355507450489 1102 78-79 0.5771944015869472 1102 80-81 0.6678448428444916 1102 82-83 0.6301006412983909 1102 84-85 0.6454880723130856 1102 86-87 0.7854885564235765 1102 88-89 0.8702478210792073 1102 90-91 0.8254814134204267 1102 92-93 1.0572035440825722 1102 94-95 1.1622804958878312 1102 96-97 0.9014724844659092 1102 98-99 1.2727014573569058 1102 100-101 1.6518986437027472 1103 1 0.07815322955189075 1103 2 0.09511889448604194 1103 3 0.017658395683234573 1103 4 0.08674587745234419 1103 5 0.1437647876962913 1103 6 -0.018993308469497094 1103 7 0.15620829365400368 1103 8 0.12654514285746643 1103 9 0.0808128868969149 1103 10-11 0.14270141156032423 1103 12-13 0.21900170020219178 1103 14-15 0.09637541980983144 1103 16-17 0.03523497203654813 1103 18-19 0.1268349302999212 1103 20-21 0.009625056985335334 1103 22-23 0.13210384732618508 1103 24-25 0.16181120242966784 1103 26-27 0.12061432693663221 1103 28-29 0.2515700219300996 1103 30-31 0.14348197855770906 1103 32-33 0.16461157065220533 1103 34-35 -0.007074672868199627 1103 36-37 -0.1895556518535102 1103 38-39 0.13144840257359647 1103 40-41 0.2577327292164 1103 42-43 0.14452802695807065 1103 44-45 0.5103886088068421 1103 46-47 0.6037912284559326 1103 48-49 0.39311132699293694 1103 50-51 0.12103808581571229 1103 52-53 0.24640195010784538 1103 54-55 0.3874275004976653 1103 56-57 0.2431637972606353 1103 58-59 -0.021112796451838278 1103 60-61 0.06938680095754535 1103 62-63 0.12794052038696435 1103 64-65 0.08229306820430793 1103 66-67 0.30741274638769056 1103 68-69 0.21102699266796776 1103 70-71 0.13565985220634502 1103 72-73 0.12334388738549507 1103 74-75 -0.0306514561859359 1103 76-77 -0.09167836148963815 1103 78-79 0.12014036555335039 1103 80-81 0.2195609935626628 1103 82-83 0.27355152095057633 1103 84-85 0.3056401934258126 1103 86-87 -0.09067063686049437 1103 88-89 0.25451702804555865 1103 90-91 0.27149086772124775 1103 92-93 0.24191733541520932 1103 94-95 0.3038316572058761 1103 96-97 0.26851554355109997 1103 98-99 0.5426242596030484 1103 100-101 1.0102517183855007 1104 1 -0.03939387805380434 1104 2 0.056240854365327664 1104 3 0.0812885344065748 1104 4 0.04009053859036982 1104 5 0.09140327856218988 1104 6 0.0262925495271773 1104 7 -0.019085257105551534 1104 8 -0.010479025943730846 1104 9 -0.01985241028888396 1104 10-11 -0.04534437305921557 1104 12-13 0.02256995867902134 1104 14-15 -0.09926591146082586 1104 16-17 0.026958911745573744 1104 18-19 0.06107448800833026 1104 20-21 -0.012785398409320692 1104 22-23 -0.024705938544492767 1104 24-25 -0.2385759717902758 1104 26-27 0.022975413391449706 1104 28-29 -0.13531254931249492 1104 30-31 -0.1475369321132476 1104 32-33 -0.06439606293557887 1104 34-35 -0.17275649681382532 1104 36-37 -0.23215749642588435 1104 38-39 -0.16327740537784052 1104 40-41 -0.15909666897284325 1104 42-43 -0.24299278999644258 1104 44-45 -0.4033848181922153 1104 46-47 -0.2279359236156182 1104 48-49 -0.39335110104592275 1104 50-51 -0.604714917320571 1104 52-53 -0.7208277253590083 1104 54-55 -0.4664057778873598 1104 56-57 -0.6619404777176001 1104 58-59 -0.7084273459179968 1104 60-61 -0.663107936685428 1104 62-63 -0.5569267160513718 1104 64-65 -0.5763595993246255 1104 66-67 -0.600307913330127 1104 68-69 -0.7937661903606106 1104 70-71 -0.8430836068318079 1104 72-73 -0.6695348121148967 1104 74-75 -0.5501665585165867 1104 76-77 -0.6053182218364093 1104 78-79 -0.5809915695569288 1104 80-81 -0.644150299582325 1104 82-83 -0.6479273493122477 1104 84-85 -0.6387267598174056 1104 86-87 -0.6053376078562422 1104 88-89 -0.5991133642853512 1104 90-91 -0.37956202636377867 1104 92-93 -0.48285927858561806 1104 94-95 -0.2759659639551373 1104 96-97 -0.3587024659676352 1104 98-99 -0.04441808440716244 1104 100-101 0.803637633021193 1105 1 0.05167572223036743 1105 2 0.08118913995424037 1105 3 0.06610504401466244 1105 4 -0.0579230708317553 1105 5 0.0561225581125413 1105 6 0.11996702385105351 1105 7 0.12766178455153465 1105 8 0.10865913685393025 1105 9 0.05904950297020406 1105 10-11 0.12258294120420743 1105 12-13 0.16730066067167826 1105 14-15 0.16283038529979876 1105 16-17 0.10692645529363887 1105 18-19 0.1300636018636112 1105 20-21 0.07407217627186213 1105 22-23 -0.010478381205921039 1105 24-25 0.1270205373999218 1105 26-27 0.038869323976015835 1105 28-29 0.00925447743970409 1105 30-31 0.019547628784387427 1105 32-33 0.16239339498596195 1105 34-35 0.11763298060943583 1105 36-37 0.1481105827232767 1105 38-39 0.17211057459108758 1105 40-41 0.1254840786399356 1105 42-43 0.3496386899759827 1105 44-45 0.2933388910369743 1105 46-47 0.2761733696555879 1105 48-49 0.30517303582837485 1105 50-51 0.2896988815469399 1105 52-53 0.3781487820203111 1105 54-55 0.40920974549033673 1105 56-57 0.5140487127516238 1105 58-59 0.2748116129681719 1105 60-61 0.06424916089983412 1105 62-63 -0.058110749705797105 1105 64-65 0.2782532564948994 1105 66-67 0.47399368093636696 1105 68-69 0.5895943060977373 1105 70-71 0.495220926624107 1105 72-73 0.4151880195364086 1105 74-75 0.4609267920897082 1105 76-77 0.05171946525832283 1105 78-79 0.4940187036762502 1105 80-81 0.5403500033909943 1105 82-83 0.5447427858765366 1105 84-85 0.41085464946123906 1105 86-87 0.17370778242214513 1105 88-89 0.5401673004450736 1105 90-91 0.5639682744504171 1105 92-93 0.2724288246621178 1105 94-95 0.2415089798032639 1105 96-97 0.12210051779717546 1105 98-99 0.01961375913323593 1105 100-101 1.0045751090793367 1106 1 -0.15064371053011882 1106 2 -0.16385352318720692 1106 3 -0.13728931296700608 1106 4 -0.05760927307774466 1106 5 -0.16909075759470937 1106 6 -0.1349569302677054 1106 7 0.0038919385127300643 1106 8 -0.03448605135795901 1106 9 -0.09188054016029668 1106 10-11 -0.025509569044295688 1106 12-13 -0.1225249478956627 1106 14-15 0.033181838784287265 1106 16-17 -0.11324606670559234 1106 18-19 -0.10318160103668106 1106 20-21 -0.196705240972328 1106 22-23 -0.15546963492810306 1106 24-25 -0.20475382198531378 1106 26-27 -0.10609188528034963 1106 28-29 -0.16013955906766597 1106 30-31 -0.35384499229481037 1106 32-33 -0.28975244752762563 1106 34-35 -0.15165556644019773 1106 36-37 -0.32079339516399585 1106 38-39 -0.07651071825565481 1106 40-41 -0.20163005027085035 1106 42-43 -0.14942993158393136 1106 44-45 -0.27423111256836563 1106 46-47 -0.15737494852197642 1106 48-49 -0.2978661288019495 1106 50-51 -0.21196357889451178 1106 52-53 -0.2167683620195362 1106 54-55 -0.10803911615823125 1106 56-57 -0.11616066379232137 1106 58-59 0.08357524981737896 1106 60-61 0.10510629612663536 1106 62-63 0.12421576019553271 1106 64-65 0.12498975985320016 1106 66-67 0.14179202248185163 1106 68-69 0.030727182755185822 1106 70-71 0.05336030623927002 1106 72-73 -0.0938052905625355 1106 74-75 0.09621034508146664 1106 76-77 -0.09194983196823969 1106 78-79 -0.13311612253320604 1106 80-81 0.10311289246682875 1106 82-83 0.2988688925143599 1106 84-85 0.03502175010352815 1106 86-87 0.05750246521935054 1106 88-89 0.0967376912901905 1106 90-91 0.43963759838317173 1106 92-93 0.41713966337007236 1106 94-95 0.3108783130831867 1106 96-97 0.23196311444020523 1106 98-99 -0.15509480954841237 1106 100-101 0.022104385742128585 1107 1 -0.02898877116750853 1107 2 -0.019611018101670652 1107 3 -0.038991161545830266 1107 4 -0.07653525211310352 1107 5 -0.2159410593921507 1107 6 0.0047958514932773255 1107 7 -0.0471557377025178 1107 8 0.0553295353963037 1107 9 0.08157795852246608 1107 10-11 -0.037582578459485205 1107 12-13 0.06839278312110508 1107 14-15 -0.013554524751022257 1107 16-17 0.01010294008624868 1107 18-19 -0.03139215894510983 1107 20-21 -0.011392716656239088 1107 22-23 -0.015183288622480973 1107 24-25 -0.03370970286702146 1107 26-27 -0.14031449285474196 1107 28-29 -0.10740615236920092 1107 30-31 -0.07977734075691956 1107 32-33 -0.014358665777763235 1107 34-35 -0.04744818768718062 1107 36-37 0.005196756123780233 1107 38-39 -0.186336087026568 1107 40-41 -0.3359782678317913 1107 42-43 -0.4077240812710201 1107 44-45 -0.3019358957521874 1107 46-47 -0.4716100059357444 1107 48-49 -0.355903500551932 1107 50-51 -0.2208101902038564 1107 52-53 -0.2359077668727494 1107 54-55 -0.3512834109081808 1107 56-57 -0.2467578114451996 1107 58-59 -0.14515831250407274 1107 60-61 -0.14857696662055275 1107 62-63 -0.14905765828581252 1107 64-65 -0.1981102501511245 1107 66-67 0.2556196811339291 1107 68-69 0.16633539075643 1107 70-71 0.1371260491723234 1107 72-73 0.004932495811559079 1107 74-75 -0.21542025039879675 1107 76-77 -0.36452517017034936 1107 78-79 -0.033140021778436335 1107 80-81 0.19169183222961195 1107 82-83 0.06342631184051228 1107 84-85 -0.1934617038376274 1107 86-87 -0.028345846826979937 1107 88-89 -0.08830471597518041 1107 90-91 0.1753225062877064 1107 92-93 0.3953941291941483 1107 94-95 0.005789441238718496 1107 96-97 -0.005429200007167623 1107 98-99 0.15853832036343718 1107 100-101 0.40230525056133004 1108 1 0.1036737564064758 1108 2 0.0366791680469305 1108 3 -0.10436155136875414 1108 4 0.028056265879790487 1108 5 -0.03179160976616657 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-1.2791347520829603 2316 56-57 -1.2342640869956512 2316 58-59 -0.8940699527536609 2316 60-61 -0.8409526210708123 2316 62-63 -0.9877509093795496 2316 64-65 -0.924152439780336 2316 66-67 -0.9143576372337066 2316 68-69 -1.080717790051139 2316 70-71 -0.9421507384630523 2316 72-73 -1.120275493860678 2316 74-75 -1.330635770731579 2316 76-77 -1.5650751773424076 2316 78-79 -1.5131976671919034 2316 80-81 -1.3575698940846657 2316 82-83 -1.5654008774300898 2316 84-85 -1.8413997372594153 2316 86-87 -1.7095971915877932 2316 88-89 -2.1060878329514416 2316 90-91 -2.1573807318963247 2316 92-93 -2.4583765801223514 2316 94-95 -2.727670897282401 2316 96-97 -3.0347902593200864 2316 98-99 -3.277892594791119 2316 100-101 -3.9328315564385967 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 21.0 5 61.0 6 138.0 7 251.0 8 486.0 9 756.0 10 1203.0 11 1903.0 12 2741.0 13 3728.0 14 4288.0 15 5533.0 16 7134.0 17 9613.0 18 9421.0 19 6690.0 20 6493.0 21 7165.0 22 7380.0 23 8321.0 24 9404.0 25 10859.0 26 12922.0 27 15493.0 28 18745.0 29 22775.0 30 27450.0 31 33180.0 32 40635.0 33 51546.0 34 68692.0 35 100129.0 36 148548.0 37 207708.0 38 123921.0 39 6369.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.40190493136704 18.512030580345503 14.235752129856024 18.850312358431427 2 14.559217201041449 25.510233185427843 35.08069609293191 24.849853520598796 3 26.864811125100335 25.025058367661256 30.677248224522973 17.432882282715433 4 12.277887111035053 15.563894763004885 40.34549988387586 31.812718242084202 5 11.154765275958383 41.49674138693255 34.02169284897922 13.326800488129848 6 27.49717838705603 37.163596507311745 16.31839610148843 19.0208290041438 7 24.435677411205774 36.0231351888749 21.146919450590197 18.39426794932913 8 23.354500523577276 28.255448667017074 20.871481132882693 27.518569676522958 9 22.342386941942003 22.161579614304863 24.52939163172756 30.966641812025568 10-11 24.930070856099775 24.181935972814728 26.990510416539337 23.89748275454616 12-13 31.583780513146475 22.815643755577014 25.66501444421355 19.935561287062956 14-15 24.45114370421529 25.443605254352015 30.635209007227765 19.470042034204933 16-17 19.335799522551472 28.55018939538636 30.859656923640323 21.254354158421844 18-19 18.44087040138208 32.223787521340356 29.989874789652323 19.34546728762524 20-21 21.934882877597005 26.624719366654855 27.016943938523468 24.423453817224676 22-23 19.677388795853755 26.970901734527985 28.37478150325759 24.97692796636067 24-25 24.171902439421906 26.78759875645304 28.02717304941999 21.013325754705065 26-27 23.68616737359785 23.028283359206608 33.34331593508457 19.942233332110973 28-29 19.49342370643817 29.95447729874871 30.66298736487835 19.889111629934767 30-31 20.31734487240605 27.66616957384477 28.956420850191705 23.060064703557472 32-33 20.677991826490157 28.013828959323956 29.74575943152139 21.5624197826645 34-35 23.811614494969586 25.273854933157853 29.002672463540762 21.9118581083318 36-37 22.80076403922068 24.53279001655418 32.55504902584999 20.111396918375142 38-39 22.49504627719454 25.882577667751644 32.01897950763306 19.603396547420754 40-41 20.674578961147176 27.717410681088218 31.032292633335334 20.575717724429268 42-43 19.053078495706924 29.402656827921447 27.87947808743431 23.66478658893732 44-45 21.259106614621107 26.473000008149093 27.72785890655432 24.54003447067548 46-47 23.02426343595597 28.487980332147128 27.28405266967063 21.203703562226274 48-49 22.70863170847484 26.787704261764723 28.625780019680025 21.87788401008041 50-51 19.33920749719874 28.4951105225629 31.87903636548844 20.286645614749922 52-53 21.000389117741733 30.043200218394876 27.68771365823646 21.268697005626926 54-55 17.974438427719853 26.66352927754485 30.228540462133342 25.133491832601955 56-57 22.117320017764957 27.029320327429335 27.60556092035514 23.24779873445057 58-59 24.531860385757433 25.968238931247168 27.198089038967915 22.301811644027485 60-61 20.821973206313828 26.726964259975055 31.448634569182726 21.00242796452839 62-63 21.266303218472295 26.4768648111251 30.101516948013057 22.155315022389548 64-65 19.88380431615983 30.205682760419105 28.735232340435935 21.175280582985128 66-67 20.41973428543052 26.8384957611472 27.593772999182537 25.147996954239744 68-69 18.823476681515224 28.48127054494855 28.55828067748544 24.136972096050783 70-71 22.41858067775754 28.051671169023784 26.12553834745158 23.404209805767092 72-73 21.047297159644213 25.20021228308214 29.664727189755 24.08776336751865 74-75 19.203418939236514 30.80804068012077 27.09160972509137 22.896930655551344 76-77 21.274935708592537 25.86852681269132 27.484136863313637 25.3724006154025 78-79 20.676729066448 26.426863398721107 29.217307599259453 23.67909993557144 80-81 22.062110118283645 27.144731427267576 27.69586272089053 23.097295733558248 82-83 20.533651554229976 26.346327013735245 29.400646628121603 23.71937480391318 84-85 20.056880457325395 26.84871672635855 28.390315653941904 24.704087162374147 86-87 20.61223240654313 27.46099269075199 27.84956108807312 24.077213814631758 88-89 20.561279270274543 26.551221605717245 26.713273692572663 26.17422543143555 90-91 20.701597135845976 24.675273573518634 28.099963308903263 26.523165981732127 92-93 21.59552534969665 27.547804438794426 27.77679309937374 23.07987711213518 94-95 20.654817929567653 26.568134312850667 27.595731113528664 25.18131664405302 96-97 17.749365772118015 26.44133092867464 29.64351325321087 26.165790045996484 98-99 20.423690140041643 27.212827032070635 26.116829036740047 26.246653791147672 100-101 20.006386837613892 25.196481840550106 27.80892252419639 26.98820879763962 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5574.0 1 3628.5 2 1666.5 3 1533.0 4 1254.5 5 1289.0 6 2058.0 7 3297.0 8 4279.5 9 4411.5 10 3475.0 11 2557.5 12 2365.0 13 2349.0 14 2335.5 15 2316.0 16 2267.5 17 2236.0 18 2300.0 19 2336.5 20 2369.5 21 2558.0 22 2930.0 23 3431.5 24 3739.5 25 4127.5 26 4778.5 27 5338.0 28 5959.5 29 6689.0 30 7441.0 31 8452.5 32 9494.5 33 10257.0 34 11133.5 35 11731.0 36 12110.5 37 13352.5 38 14720.5 39 15044.5 40 15085.5 41 15964.0 42 17359.0 43 18761.5 44 20474.0 45 22570.0 46 31320.5 47 65116.0 48 134639.0 49 129309.5 50 62388.0 51 43079.5 52 39198.5 53 31495.0 54 23041.0 55 17669.0 56 15054.5 57 13269.0 58 11883.5 59 10762.0 60 9251.0 61 7465.5 62 5933.0 63 4411.0 64 2999.0 65 2174.0 66 1467.0 67 1052.0 68 741.0 69 452.5 70 315.5 71 216.5 72 139.5 73 81.5 74 53.0 75 44.5 76 32.0 77 14.5 78 7.0 79 8.0 80 4.5 81 2.0 82 2.0 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38962705814763654 2 0.0 3 0.0 4 0.0 5 2.0372656635170538E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.639746238188952E-4 16-17 0.0032086934200393604 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.003004966853687655 36-37 0.008454652503595774 38-39 0.012529183830629882 40-41 0.0029540352120997282 42-43 0.0011204961149343796 44-45 4.0745313270341076E-4 46-47 4.5838477429133715E-4 48-49 2.0372656635170538E-4 50-51 8.149062654068215E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 3.055898495275581E-4 60-61 0.002190060588280833 62-63 0.0 64-65 2.0372656635170538E-4 66-67 0.0015788808892257167 68-69 0.0019354023803412012 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12340736756754556 78-79 5.0931641587926345E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14698871762275545 88-89 0.4323587054399068 90-91 0.33299107270186246 92-93 0.0 94-95 0.0 96-97 2.5465820793963177E-4 98-99 0.0 100-101 1.5279492476377905E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 981708.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.819530493283928 #Duplication Level Percentage of deduplicated Percentage of total 1 67.60527202402919 19.483521986032642 2 14.706198382114705 8.476514654272751 3 6.468038443126274 5.592174934302312 4 3.344362630975495 3.8553184329599017 5 2.0914433610593854 3.0137207859513593 6 1.3374283947849361 2.312643504365295 7 0.8357535760246976 1.6860217968380404 8 0.6119877734369579 1.4109760238466678 9 0.46575128633256524 1.2080460058872822 >10 2.255568498014374 11.453199261014642 >50 0.15694900490145997 3.1183568644204 >100 0.09402799505303262 4.996094144893709 >500 0.00671628536093058 1.4657600129833355 >1k 0.011665127205826797 7.888189016215781 >5k 0.00671628536093058 12.921059597353931 >10k+ 0.002120932219241236 11.118402978661958 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 37514 3.821299205058938 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 19056 1.9411067241990492 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14276 1.4542002306184731 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 13770 1.4026574093314916 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 12311 1.2540388791779224 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12212 1.243954414143513 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9514 0.9691272761350626 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 9145 0.9315397246431729 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9030 0.9198254470779498 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8856 0.9021012358053514 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8677 0.8838677081168738 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8122 0.8273335859542756 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 7463 0.7602056823413886 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6839 0.6966429936396565 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6199 0.6314504924071109 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6078 0.6191250351428327 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 5739 0.5845933821462187 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5366 0.5465983775216255 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5255 0.5352915530891059 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5195 0.5291797560985548 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5156 0.5252070880546965 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 5079 0.5173636152501558 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5067 0.5161412558520456 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5043 0.5136965370558252 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 5011 0.5104369119941978 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4956 0.504834431419526 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4565 0.4650058876977676 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 4388 0.4469760865756417 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3742 0.38117240564404076 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3654 0.37220843672456577 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3556 0.36222583497333216 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3374 0.34368671743532697 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 3261 0.33217616643645564 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3161 0.3219898381188704 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3152 0.3210730685702877 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2880 0.2933662555464558 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2777 0.2828743373793429 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 2640 0.2689190675842511 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2542 0.2589364658330176 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 2235 0.22766443789803076 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2078 0.21167190243942194 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1966 0.20026321472372638 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1782 0.18152037061936951 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1709 0.17408435094753225 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1674 0.17051913603637742 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1603 0.16328684293089188 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1595 0.16247193666548504 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1554 0.15829554205527507 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1539 0.1567675928076373 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1394 0.14199741674713867 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1379 0.14046946749950087 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1309 0.13333903767719119 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1261 0.12844960008475026 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1245 0.1268197875539366 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1154 0.11755022878493403 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1123 0.11439246700648259 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1112 0.1132719708915482 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1071 0.10909557628133823 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.0186328317585269E-4 0.0 9 0.0 0.0 0.0 2.0372656635170538E-4 0.0 10-11 0.0 0.0 0.0 3.055898495275581E-4 0.0 12-13 0.0 0.0 0.0 4.0745313270341076E-4 0.0 14-15 0.0 0.0 0.0 4.5838477429133715E-4 0.0 16-17 0.0 0.0 0.0 7.639746238188951E-4 0.0 18-19 0.0 0.0 0.0 9.167695485826743E-4 0.0 20-21 0.0 0.0 0.0 0.0016807441724015697 0.0 22-23 0.0 0.0 0.0 0.0035142832695669183 0.0 24-25 0.0 0.0 0.0 0.006111796990551161 0.0 26-27 0.0 0.0 0.0 0.010848439658228314 0.0 28-29 0.0 0.0 0.0 0.030304326744816177 0.0 30-31 0.0 0.0 0.0 0.0773142319304722 0.0 32-33 0.0 0.0 0.0 0.18309925150859524 0.0 34-35 0.0 0.0 0.0 0.3535165242617968 0.0 36-37 0.0 0.0 0.0 0.5871908958672029 0.0 38-39 0.0 0.0 0.0 0.9035782534114014 0.0 40-41 0.0 0.0 0.0 1.2283184001760197 0.0 42-43 0.0 0.0 0.0 1.5631939436166356 0.0 44-45 0.0 0.0 0.0 1.9371849877967786 0.0 46-47 0.0 0.0 0.0 2.413446768285478 0.0 48-49 0.0 0.0 0.0 2.874021603165096 0.0 50-51 0.0 0.0 0.0 3.3661231241876406 0.0 52-53 0.0 0.0 0.0 3.7964445639640303 0.0 54-55 0.0 0.0 0.0 4.260992066887507 0.0 56-57 0.0 0.0 0.0 4.89213696944509 0.0 58-59 0.0 0.0 0.0 5.5032657368586175 0.0 60-61 0.0 0.0 0.0 6.2947434471349935 0.0 62-63 0.0 0.0 0.0 7.0083976090650175 0.0 64-65 0.0 0.0 0.0 7.613974827545462 0.0 66-67 0.0 0.0 0.0 8.327119673059606 0.0 68-69 0.0 0.0 0.0 9.169732751490258 0.0 70-71 0.0 0.0 0.0 10.05074828767821 0.0 72-73 0.0 0.0 0.0 10.903089309652158 0.0 74-75 0.0 0.0 0.0 11.834781829220095 0.0 76-77 0.0 0.0 0.0 12.626208607854881 0.0 78-79 0.0 0.0 0.0 13.77176309045052 0.0 80-81 0.0 0.0 0.0 14.967892693142971 0.0 82-83 0.0 0.0 0.0 15.97516776882739 0.0 84-85 0.0 0.0 0.0 17.19365636217694 0.0 86-87 0.0 0.0 0.0 18.4209561295212 0.0 88-89 0.0 0.0 0.0 19.5970695970696 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTAACG 25 0.0016238082 37.96319 20-21 AACATCG 60 0.003969463 31.635986 6 GTAAGCG 195 0.0 27.985683 94-95 TCGTATA 35 0.008135319 27.31691 84-85 CCTATAC 160 6.295704E-8 26.694227 3 TTTGCGT 765 0.0 25.434149 3 CTCCGAC 195 0.0 24.335377 94-95 CATGGGG 1145 0.0 24.03905 4 CTTTGCG 810 0.0 24.021141 2 GTCTTAC 100 0.0016290983 23.81067 1 CTAGACT 145 2.3505194E-5 22.909986 4 GCTAATC 105 0.0022077449 22.598286 3 GGGTATA 110 0.002834785 21.646063 1 TACATGG 5375 0.0 21.454708 2 GTACATG 5465 0.0 21.087584 1 GCTTTGC 950 0.0 21.053646 1 GAGTACT 4370 0.0 20.686462 12-13 ATGGGGA 460 0.0 20.633217 5 CCATATA 115 0.0037427554 20.633217 2 ACATGGG 5430 0.0 20.188635 3 >>END_MODULE