FastQCFastQC Report
Fri 27 May 2016
SRR939053_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939053_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2623182
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89260.34027375912155544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71720.27340840246692755No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT63650.24264423894339013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43930.16746836475700122No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32100.12237046457317868No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG109200.025.9500521
TACATGG117000.023.9649522
ACATGGG113350.023.6502463
GTATCAA188250.023.626051
GAGTACT79700.022.24451612-13
CATGGGG59850.021.208574
GGTATCA141800.020.4527421
AGTACTT83800.020.45003312-13
TATCAAC215900.020.3799842
ATCAACG216600.020.379723
CAACGCA216500.020.3012375
TCAACGC217500.020.2518394
AACGCAG219800.020.029226
GTACTTT90250.019.77607714-15
GTATAGG6750.018.986571
CATGGGA55600.018.8260084
ACGCAGA240800.018.066247
TTTGCGT4253.6379788E-1217.8308413
GTATATG9350.017.7681831
CGCAGAG242250.017.7431558
Produced by FastQC (version 0.11.5)