##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939053_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2623182 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.862864643017527 33.0 31.0 34.0 30.0 34.0 2 31.937722963942267 33.0 31.0 34.0 30.0 34.0 3 31.987724069469827 34.0 31.0 34.0 30.0 34.0 4 35.52404026865082 37.0 35.0 37.0 33.0 37.0 5 35.56364865266688 37.0 35.0 37.0 33.0 37.0 6 35.64859434076629 37.0 35.0 37.0 33.0 37.0 7 35.57018232055572 37.0 35.0 37.0 33.0 37.0 8 35.56238148935148 37.0 35.0 37.0 33.0 37.0 9 37.17816605938894 39.0 37.0 39.0 33.0 39.0 10-11 37.06515636353101 39.0 37.0 39.0 33.0 39.0 12-13 36.95877335236366 39.0 37.0 39.0 33.0 39.0 14-15 38.1888023781804 40.0 38.0 41.0 33.0 41.0 16-17 38.10992641761037 40.0 38.0 41.0 33.0 41.0 18-19 38.019220359090596 40.0 38.0 41.0 33.0 41.0 20-21 38.12736478063665 40.0 38.0 41.0 33.0 41.0 22-23 38.06390216157324 40.0 38.0 41.0 33.0 41.0 24-25 37.98367040487469 40.0 38.0 41.0 33.0 41.0 26-27 37.8713238349455 40.0 38.0 41.0 33.0 41.0 28-29 37.74930447067722 40.0 38.0 41.0 32.0 41.0 30-31 37.558082893218995 40.0 37.0 41.0 32.0 41.0 32-33 37.370249376520576 40.0 37.0 41.0 31.5 41.0 34-35 37.05588518066989 40.0 36.5 41.0 30.5 41.0 36-37 36.79838798832868 40.0 36.0 41.0 30.0 41.0 38-39 36.56521373659929 39.5 35.5 41.0 30.0 41.0 40-41 36.41213457548885 39.0 35.5 41.0 30.0 41.0 42-43 36.20120601620475 39.0 35.0 41.0 29.5 41.0 44-45 36.14715505824606 39.0 35.0 41.0 29.5 41.0 46-47 35.92871177066631 39.0 35.0 41.0 28.0 41.0 48-49 35.69245671859596 39.0 35.0 41.0 27.0 41.0 50-51 34.56727630793441 38.0 34.0 40.0 25.5 40.5 52-53 34.65629624631459 38.0 34.5 39.5 26.0 40.5 54-55 35.137792574057 39.0 35.0 40.0 26.0 41.0 56-57 35.042470556751304 39.0 35.0 40.5 26.0 41.0 58-59 34.82657131682056 38.0 34.5 40.0 26.0 41.0 60-61 34.50949152594063 38.0 34.0 40.0 25.5 41.0 62-63 34.13096918170375 37.5 34.0 40.0 23.5 41.0 64-65 33.73627735322978 37.0 33.5 40.0 23.0 41.0 66-67 33.32608888746568 36.0 33.0 39.0 22.0 41.0 68-69 32.889736015266955 36.0 33.0 39.0 21.5 40.5 70-71 32.44330435326256 35.0 32.0 38.5 20.0 40.0 72-73 32.00400448767947 35.0 32.0 37.5 18.5 40.0 74-75 31.545762169761762 35.0 31.0 37.0 17.5 39.0 76-77 31.131811860557143 35.0 31.0 36.5 13.5 39.0 78-79 30.755124501464252 35.0 31.0 36.0 9.5 38.5 80-81 30.3174802587087 34.5 30.5 35.5 7.0 37.0 82-83 29.870037420201875 34.0 30.0 35.0 2.0 37.0 84-85 29.474352904220904 34.0 29.5 35.0 2.0 36.5 86-87 29.046801175061432 34.0 29.0 35.0 2.0 36.0 88-89 28.587135204495915 34.0 29.0 35.0 2.0 36.0 90-91 28.145531457596157 34.0 28.5 35.0 2.0 35.0 92-93 27.658014579240024 34.0 27.0 35.0 2.0 35.0 94-95 27.137028235173922 33.0 26.0 35.0 2.0 35.0 96-97 26.499857425066196 33.0 25.0 35.0 2.0 35.0 98-99 25.677901304598763 33.0 22.0 35.0 2.0 35.0 100-101 22.529527497520185 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.041922840085838686 1101 2 0.16596774566913197 1101 3 0.19629668156532176 1101 4 0.1337239893532569 1101 5 -0.0305828550381122 1101 6 0.13652955656407073 1101 7 0.20079942045107657 1101 8 0.08200948803286678 1101 9 0.1880311372131942 1101 10-11 0.07016711535167275 1101 12-13 0.1239345159650398 1101 14-15 0.24113182010862744 1101 16-17 0.2605923808349502 1101 18-19 0.34198625677429817 1101 20-21 0.23348383177793863 1101 22-23 0.300767669384264 1101 24-25 0.14677770959941228 1101 26-27 0.24993143566506149 1101 28-29 0.29335915115151323 1101 30-31 0.1167596651673719 1101 32-33 0.29384592832044376 1101 34-35 0.058464583858835795 1101 36-37 0.17655960812752625 1101 38-39 0.26320246721756035 1101 40-41 0.2150176077182735 1101 42-43 0.23901329655408432 1101 44-45 0.446605363215987 1101 46-47 0.3862045845462063 1101 48-49 0.3812637275881485 1101 50-51 0.3472838738219082 1101 52-53 0.3089511881986553 1101 54-55 0.33407871655829524 1101 56-57 0.3940352544880952 1101 58-59 0.2949452504859238 1101 60-61 0.26584566941542676 1101 62-63 0.23098367656380958 1101 64-65 0.1817670132289848 1101 66-67 0.273277093808197 1101 68-69 0.21941498431994688 1101 70-71 0.1309008511070502 1101 72-73 0.07598028173208604 1101 74-75 -0.13203752110990052 1101 76-77 -0.0957903674680729 1101 78-79 -0.18781187646425934 1101 80-81 -0.3027210224076633 1101 82-83 -0.15800509587266376 1101 84-85 -0.2292866645915943 1101 86-87 -0.25704884704860476 1101 88-89 -0.41197947172285865 1101 90-91 -0.4361353932694563 1101 92-93 -0.4576126093934647 1101 94-95 -0.853266052404269 1101 96-97 -0.7926174195782174 1101 98-99 -0.9560149411841401 1101 100-101 -0.6711975471257894 1102 1 0.29444973289265874 1102 2 0.27091155430919756 1102 3 0.23154172400955986 1102 4 0.15227057491190976 1102 5 0.2465659919220613 1102 6 0.3044867342799833 1102 7 0.3410948117285315 1102 8 0.28511290075582707 1102 9 0.30679595832877027 1102 10-11 0.34719524539944047 1102 12-13 0.32033491806835457 1102 14-15 0.43581769837786766 1102 16-17 0.5534507511136795 1102 18-19 0.583022703830693 1102 20-21 0.4814252003038959 1102 22-23 0.5665248780312098 1102 24-25 0.5583171294708791 1102 26-27 0.5176725258118111 1102 28-29 0.4160333099995057 1102 30-31 0.49870282081916173 1102 32-33 0.45256804743925017 1102 34-35 0.3827544591037082 1102 36-37 0.34548724671569175 1102 38-39 0.35756485852937914 1102 40-41 0.44465167652261783 1102 42-43 0.21419114994103694 1102 44-45 0.32744402355898217 1102 46-47 0.4312895589421899 1102 48-49 0.4146305695306154 1102 50-51 0.31574711745526685 1102 52-53 0.2799954539305176 1102 54-55 0.22856311357517 1102 56-57 0.3286857339189666 1102 58-59 0.24217778304942073 1102 60-61 0.39394607495551526 1102 62-63 0.25493116255893256 1102 64-65 0.09775964133496728 1102 66-67 0.05814151282046964 1102 68-69 0.13492001920327112 1102 70-71 0.10581020602335656 1102 72-73 -0.017838239077619278 1102 74-75 0.06595539912242643 1102 76-77 -7.60811156425234E-4 1102 78-79 -0.10169818776783046 1102 80-81 -0.15923071412845857 1102 82-83 -0.07337265953902161 1102 84-85 -0.09664025774553053 1102 86-87 -0.19635014958838326 1102 88-89 -0.4526666559686596 1102 90-91 -0.33948281740252995 1102 92-93 -0.1992109700492044 1102 94-95 -0.20114813646791774 1102 96-97 -0.2721917743226925 1102 98-99 -0.2686549899864197 1102 100-101 -0.2728626672412666 1103 1 0.1965703661208451 1103 2 0.25527336514042176 1103 3 0.2329855909072407 1103 4 0.32708953621627046 1103 5 0.18265451947399924 1103 6 0.18886631578071444 1103 7 0.17524633630388564 1103 8 0.16171411212313558 1103 9 0.23328091989969835 1103 10-11 0.3019948070562606 1103 12-13 0.2646405291572549 1103 14-15 0.3450984305294469 1103 16-17 0.4217201100367731 1103 18-19 0.43304380650073426 1103 20-21 0.4012443096971836 1103 22-23 0.4911272593009244 1103 24-25 0.5412091578447118 1103 26-27 0.4341133568867903 1103 28-29 0.28708859159057454 1103 30-31 0.44899492619922654 1103 32-33 0.5508768720575503 1103 34-35 0.46299088305264746 1103 36-37 0.34046703257912014 1103 38-39 0.5099020717922187 1103 40-41 0.4211221994245662 1103 42-43 0.2615499841851161 1103 44-45 0.47210838003861255 1103 46-47 0.5610228508106303 1103 48-49 0.6338650979414737 1103 50-51 0.44414390688764627 1103 52-53 0.6652950378006821 1103 54-55 0.6375069434506315 1103 56-57 0.4884422017022203 1103 58-59 0.40903696530244815 1103 60-61 0.5108178447661089 1103 62-63 0.3582083701943972 1103 64-65 0.29384602159768036 1103 66-67 0.2966442598698009 1103 68-69 0.3292793125229778 1103 70-71 0.3190378779175589 1103 72-73 0.2721161495373039 1103 74-75 0.17316436738697405 1103 76-77 0.3429331927235033 1103 78-79 0.21361642712695428 1103 80-81 0.2622414377515305 1103 82-83 0.41994482828066637 1103 84-85 0.3920013226593433 1103 86-87 0.40243070843877504 1103 88-89 0.405166136840144 1103 90-91 0.47135766122083567 1103 92-93 0.3972541476074376 1103 94-95 0.2579626636564747 1103 96-97 0.0584222763533333 1103 98-99 0.038877552552119 1103 100-101 0.2958653970885585 1104 1 0.12897183048637118 1104 2 0.1386423567250752 1104 3 0.17916659219093845 1104 4 0.24462269839000328 1104 5 0.25343733695130055 1104 6 0.10232730565377324 1104 7 0.19582590174866255 1104 8 0.19975195741386642 1104 9 0.1833058806756256 1104 10-11 0.26429571515306094 1104 12-13 0.2593987662133088 1104 14-15 0.3921869343092226 1104 16-17 0.44107897467805657 1104 18-19 0.40206570066705183 1104 20-21 0.35075460304572914 1104 22-23 0.4076031543230343 1104 24-25 0.36332024518365813 1104 26-27 0.3252748649930979 1104 28-29 0.33975107435575325 1104 30-31 0.4623364609303664 1104 32-33 0.44872014215692246 1104 34-35 0.43268255803957345 1104 36-37 0.38640734066641613 1104 38-39 0.5053491074029282 1104 40-41 0.4217248238718625 1104 42-43 0.41366986722605503 1104 44-45 0.2300048997932791 1104 46-47 0.3773002482337233 1104 48-49 0.31775826582051536 1104 50-51 0.25621303635087145 1104 52-53 0.3341249717140542 1104 54-55 0.52426822334413 1104 56-57 0.4281464759379432 1104 58-59 0.3893221124521631 1104 60-61 0.4223170365124105 1104 62-63 0.33829085961578187 1104 64-65 0.2793961095547033 1104 66-67 0.29690337649605425 1104 68-69 0.3933060799836312 1104 70-71 0.20832554491704514 1104 72-73 0.17981179447621543 1104 74-75 0.13803861262065453 1104 76-77 0.03164260174741784 1104 78-79 0.12476260218519641 1104 80-81 0.18286735892302275 1104 82-83 0.2722953013467979 1104 84-85 0.2659963542763286 1104 86-87 0.2360784286879074 1104 88-89 0.13759434489415412 1104 90-91 0.21889605327142192 1104 92-93 0.2031330046416251 1104 94-95 0.3660446063180096 1104 96-97 0.218409558243696 1104 98-99 0.33774540307272716 1104 100-101 0.3638032804147606 1105 1 0.2568047417632613 1105 2 0.25812083184655776 1105 3 0.2785203895582171 1105 4 0.2502925133695797 1105 5 0.21857148245810976 1105 6 0.16018725135678125 1105 7 0.17161240963085334 1105 8 0.21015393765706136 1105 9 0.13072278118437453 1105 10-11 0.31907578952624505 1105 12-13 0.27725061299540954 1105 14-15 0.35854989144197447 1105 16-17 0.35592794691357454 1105 18-19 0.41184286627287037 1105 20-21 0.3209528555039256 1105 22-23 0.2619141186649685 1105 24-25 0.32157743725355914 1105 26-27 0.3501750543481492 1105 28-29 0.431139982256191 1105 30-31 0.42057312052473605 1105 32-33 0.37160158992458747 1105 34-35 0.41344419881250616 1105 36-37 0.3069634855800629 1105 38-39 0.423065496881172 1105 40-41 0.47828209807166644 1105 42-43 0.4008362144986535 1105 44-45 0.5539369258923372 1105 46-47 0.44602672143884803 1105 48-49 0.36053824893826913 1105 50-51 0.3767215573282172 1105 52-53 0.4133917341989388 1105 54-55 0.5168423113600085 1105 56-57 0.5178152186660228 1105 58-59 0.43100674503492087 1105 60-61 0.5851324381504455 1105 62-63 0.6398655864197877 1105 64-65 0.6160015210026941 1105 66-67 0.6073156229770191 1105 68-69 0.562136071086222 1105 70-71 0.44071010677682665 1105 72-73 0.5257597674645993 1105 74-75 0.5048980564187033 1105 76-77 0.3555623653958122 1105 78-79 0.28884816557350845 1105 80-81 0.3688111683816473 1105 82-83 0.4464280894288386 1105 84-85 0.4130433850522266 1105 86-87 0.31209735278619277 1105 88-89 0.3502842702928284 1105 90-91 0.26504906584186116 1105 92-93 0.48607508024675994 1105 94-95 0.3285035413669455 1105 96-97 0.2718222267811008 1105 98-99 0.37824941831238945 1105 100-101 0.5652796224401762 1106 1 0.020364374346016234 1106 2 0.03834082163735886 1106 3 0.030071197454098808 1106 4 0.09067532996894556 1106 5 0.1050272492320019 1106 6 0.1635389284607882 1106 7 0.15591362104690631 1106 8 0.0822080977647417 1106 9 0.14972583681597484 1106 10-11 0.061664136205692444 1106 12-13 0.1917589635817265 1106 14-15 0.16607673694080205 1106 16-17 0.148644764151733 1106 18-19 0.04885846382494208 1106 20-21 0.11705120392291946 1106 22-23 0.23427530344584113 1106 24-25 0.16248417062225684 1106 26-27 0.027974974642219763 1106 28-29 0.08470721470663278 1106 30-31 -0.019996324157354195 1106 32-33 0.2112886728586787 1106 34-35 0.260355255407994 1106 36-37 0.14608387575413673 1106 38-39 0.3886276161748654 1106 40-41 0.31343260895958025 1106 42-43 0.2512246917874492 1106 44-45 0.37579711470556276 1106 46-47 0.2596467394618003 1106 48-49 0.4346168184520991 1106 50-51 0.12127608459648798 1106 52-53 0.26730753750352676 1106 54-55 0.3000357672037808 1106 56-57 0.38571226541162673 1106 58-59 0.4955721124521659 1106 60-61 0.6856119926527597 1106 62-63 0.4784498508438588 1106 64-65 0.4334668332389171 1106 66-67 0.443504253689035 1106 68-69 0.3396328343696027 1106 70-71 0.4414806984258135 1106 72-73 0.355867167283229 1106 74-75 0.4556921213925875 1106 76-77 0.4099320754316267 1106 78-79 0.4992280627115093 1106 80-81 0.5331551878703884 1106 82-83 0.5612755645046938 1106 84-85 0.48507530164474844 1106 86-87 0.5447270032493101 1106 88-89 0.5955576124380144 1106 90-91 0.7451844304644055 1106 92-93 0.6782426537644319 1106 94-95 0.5333006370197637 1106 96-97 0.6487275406998343 1106 98-99 0.7126357539499217 1106 100-101 1.0291678637480963 1107 1 0.20431674617364592 1107 2 0.3021068431839389 1107 3 0.37610067497070787 1107 4 0.34702007532749235 1107 5 0.3288205766038246 1107 6 0.2661579988917211 1107 7 0.2454000799125211 1107 8 0.3278008417606557 1107 9 0.33443005032304285 1107 10-11 0.2754054068580487 1107 12-13 0.35564514672770287 1107 14-15 0.44610449520174456 1107 16-17 0.37431436898906867 1107 18-19 0.3696393678446981 1107 20-21 0.33580186926953814 1107 22-23 0.27758271487490305 1107 24-25 0.19264233682051923 1107 26-27 0.2495360641073887 1107 28-29 0.31378871700606936 1107 30-31 0.36716953877059666 1107 32-33 0.388606873548504 1107 34-35 0.428681961889005 1107 36-37 0.47874254761583046 1107 38-39 0.4862233196325363 1107 40-41 0.30281833434708005 1107 42-43 0.5041335872056081 1107 44-45 0.15891607335826308 1107 46-47 0.4754585687924049 1107 48-49 0.5418853310564273 1107 50-51 0.47155113888877054 1107 52-53 0.5959947553965534 1107 54-55 0.6186622788712981 1107 56-57 0.4507512281096311 1107 58-59 0.5466015367410435 1107 60-61 0.5538959838808353 1107 62-63 0.5967817470284871 1107 64-65 0.5415597103041492 1107 66-67 0.25070809460199683 1107 68-69 0.45636390658898307 1107 70-71 0.3481185955046797 1107 72-73 0.5924118626077046 1107 74-75 0.6055441483045243 1107 76-77 0.6512985377038163 1107 78-79 0.5683263051318832 1107 80-81 0.582599091166248 1107 82-83 0.6288827651519462 1107 84-85 0.7082698170594988 1107 86-87 0.785073489154609 1107 88-89 0.7765165898268727 1107 90-91 0.6865740467989312 1107 92-93 0.5027029245871155 1107 94-95 0.6308610771066334 1107 96-97 0.8155991341251472 1107 98-99 0.7992851748322245 1107 100-101 0.7284238731587571 1108 1 0.20414588235702524 1108 2 0.20419039788594162 1108 3 0.2387099327068043 1108 4 0.11539105117305581 1108 5 0.18318491729250042 1108 6 0.21176148595657907 1108 7 0.24916826657845093 1108 8 0.23648134675669752 1108 9 0.3096428266075364 1108 10-11 0.33743323568726424 1108 12-13 0.37892386595090244 1108 14-15 0.392242136664521 1108 16-17 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-1.6591691088465446 2316 76-77 -1.6625149084934847 2316 78-79 -1.6509592519725373 2316 80-81 -1.6678953479284004 2316 82-83 -1.530994911752682 2316 84-85 -1.6152097429419925 2316 86-87 -1.4981793585872474 2316 88-89 -1.6828487004122827 2316 90-91 -1.899468187620215 2316 92-93 -1.7529940367607004 2316 94-95 -1.8545089815713531 2316 96-97 -2.062066119923344 2316 98-99 -2.2166987907920976 2316 100-101 -2.021404039303473 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6823.0 3 1896.0 4 1969.0 5 2172.0 6 2601.0 7 3233.0 8 4001.0 9 4868.0 10 6224.0 11 8060.0 12 10913.0 13 16038.0 14 14046.0 15 13993.0 16 17767.0 17 23741.0 18 20540.0 19 15119.0 20 15438.0 21 16643.0 22 17966.0 23 20276.0 24 23294.0 25 26600.0 26 31061.0 27 36038.0 28 43234.0 29 51004.0 30 61960.0 31 75557.0 32 93420.0 33 120412.0 34 161596.0 35 231754.0 36 356783.0 37 536574.0 38 462261.0 39 67299.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.99083907452928 18.834972919199735 14.049620914692706 23.124567091578275 2 15.483968943691744 20.758069710331398 39.7129031421084 24.045058203868454 3 19.257980464975706 24.70002182769553 30.359233496812088 25.682764210516673 4 12.95286049189218 16.463355761455155 37.03956424491036 33.54421950174231 5 13.785058232399084 37.471857043897096 34.01088887659082 14.732195847112997 6 31.985889565403802 35.64345672080397 17.465553557628414 14.905100156163817 7 28.13749738704002 33.21350591330008 20.9740962210114 17.674900478648492 8 26.114066561333455 34.59833638626721 19.98278099516235 19.30481605723698 9 26.673899335441337 16.727072420910805 20.775268850429097 35.823759393218765 10-11 25.229641878637548 26.13577389518134 27.258516265803046 21.376067960378062 12-13 26.201253564905063 23.840906390404047 27.749509023908825 22.20833102078206 14-15 23.110033912308616 24.892961951499736 26.4247851848546 25.57221895133705 16-17 22.411467904343855 28.28672812457385 26.828064400156016 22.473739570926284 18-19 22.266550314960256 27.082156702859677 28.80421917991121 21.847073802268856 20-21 23.07829643421132 26.068724706144458 28.664389055334283 22.188589804309938 22-23 22.95678281980969 26.053606442723986 28.58088832029254 22.40872241717378 24-25 22.89656780339303 26.251187925695234 28.598706537087608 22.253537733824132 26-27 22.72161605647437 26.244930220720814 28.775894531420658 22.257559191384154 28-29 22.276892634737415 26.64615047673337 28.798137403147138 22.27881948538207 30-31 22.497672171108444 26.315178016672615 28.657028250046878 22.53012156217206 32-33 22.39479146180931 26.574406067064967 28.548762043228106 22.48204042789762 34-35 22.917616443972573 26.385616725463617 28.331308841572557 22.365457988991253 36-37 22.948520134424907 26.144157723589075 28.34848634045395 22.55883580153207 38-39 22.70852605390622 26.34588387161027 28.59641965874334 22.349170415740172 40-41 22.729447836196595 26.362208747126992 28.525696200665262 22.382647216011158 42-43 22.51319230005254 26.580839698589777 28.0904958550252 22.815472146332482 44-45 22.642342917206317 26.463236129392147 28.044193002059504 22.850227951342028 46-47 22.870020869988306 26.656693002376247 27.639584209301365 22.83370191833408 48-49 23.000389689363548 26.70812879750752 27.50306444511798 22.78841706801095 50-51 22.510025991999896 26.962346180792974 27.70850260446851 22.819125222738624 52-53 22.99596796905264 26.96845528729418 27.29692231472218 22.738654428931003 54-55 22.528075423583356 26.655197171615626 27.504700947698797 23.312026457102224 56-57 22.686750338417834 27.105506692131314 27.27178533698909 22.935957632461758 58-59 23.151499576998685 26.59613580927294 27.327608294272576 22.9247563194558 60-61 22.86886703446866 26.51296261806928 27.89351211600865 22.724658231453407 62-63 22.695744182830314 26.588216905659163 27.8686091008312 22.847429810679326 64-65 22.50034295155991 26.74020135663207 27.687858901681345 23.071596790126677 66-67 22.5611762174359 26.793869741491456 27.31643206737666 23.328521973695985 68-69 22.362823836927422 26.67783890891461 27.74817037765245 23.21116687650552 70-71 22.56283839486002 26.66243930594632 27.514311896436944 23.260410402756715 72-73 22.661974578261955 26.419956474403705 27.522026258680306 23.396042688654028 74-75 22.394378800208546 26.950595684156813 27.297580723504716 23.35744479212992 76-77 22.687993563619123 26.480477748032925 27.358564840311665 23.472963848036283 78-79 22.594450777424356 26.690445984788592 27.343553690390827 23.371549547396224 80-81 22.613804411802167 26.862746337368353 27.277414599885347 23.246034650944132 82-83 22.65881947304403 26.553313348210438 27.46716342147858 23.320703757266955 84-85 22.579210361680353 26.478306725874663 27.282219633266457 23.660263279178526 86-87 22.564511009678494 26.304007073593688 27.567534641511426 23.56394727521639 88-89 22.735272352360507 26.22549127598522 27.303740078553584 23.73549629310069 90-91 22.65065817935325 26.276018805233857 27.414176884000796 23.65914613141209 92-93 22.807057545854235 26.30781764762012 27.310646138234674 23.574478668290975 94-95 22.808113472415812 25.979740463006145 27.31636249640235 23.895783568175695 96-97 22.62283837134555 25.815845668503812 27.50336021827288 24.057955741877755 98-99 22.65621048081389 25.893507242022583 27.22085371405622 24.2294285631073 100-101 23.032787701757503 25.204017985765866 27.695317049696865 24.067877262779763 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2308.0 1 1354.0 2 474.0 3 543.0 4 508.5 5 549.5 6 820.0 7 1516.0 8 2466.0 9 3021.5 10 2983.0 11 2968.0 12 3265.5 13 3545.5 14 3855.0 15 4163.0 16 4360.5 17 4502.5 18 4655.0 19 4800.0 20 5045.0 21 5738.5 22 6777.5 23 7896.5 24 9153.0 25 10935.5 26 13578.0 27 16671.0 28 19308.5 29 22235.5 30 25940.5 31 30537.5 32 35325.5 33 39466.0 34 44777.0 35 49264.0 36 53413.0 37 58791.0 38 62666.0 39 63970.5 40 65540.0 41 70721.0 42 75282.5 43 79830.0 44 86197.5 45 90365.0 46 93087.0 47 99822.0 48 116470.5 49 118663.5 50 105846.5 51 100359.5 52 96733.0 53 96793.5 54 96275.0 55 90972.0 56 83585.0 57 75100.5 58 68237.5 59 60930.0 60 51718.0 61 41357.0 62 32851.5 63 26435.5 64 20351.5 65 15079.0 66 10365.0 67 7660.5 68 5241.5 69 2837.0 70 1850.5 71 1233.0 72 749.5 73 453.0 74 331.0 75 256.0 76 160.0 77 100.5 78 66.0 79 44.5 80 35.0 81 30.5 82 28.0 83 27.0 84 20.5 85 13.0 86 12.0 87 14.0 88 8.0 89 7.0 90 7.5 91 6.5 92 7.5 93 4.5 94 3.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3278079828239138 2 0.09126320628915569 3 0.10132731926339841 4 0.10715993019165272 5 0.11730028644600338 6 0.0600797047250248 7 0.06229075984815389 8 0.06328192248955658 9 0.06644601861403442 10-11 0.06307225346925986 12-13 0.06169987442731766 14-15 0.05975567078456623 16-17 0.061166171466562363 18-19 0.06936232407816156 20-21 0.0712302844408051 22-23 0.08779413704424627 24-25 0.09692426983716723 26-27 0.09082480742853527 28-29 0.08863281312543315 30-31 0.08300987121747558 32-33 0.07424189400506713 34-35 0.3253300762204071 36-37 0.5050736090747802 38-39 0.477931001356368 40-41 0.45850802574888055 42-43 0.4899583787933891 44-45 0.49884072092595944 46-47 0.49460921887997095 48-49 0.51124931476352 50-51 0.5085617391397166 52-53 0.508199583559204 54-55 0.49824983550512314 56-57 0.4766729872345876 58-59 0.46047129021165895 60-61 0.4192427364933123 62-63 0.48982495305320023 64-65 0.5140321944874584 66-67 0.5235054220408649 68-69 0.5351134614372925 70-71 0.533283622714703 72-73 0.5216184008581943 74-75 0.5223998944793004 76-77 0.49632469268239865 78-79 0.4769017170749113 80-81 0.4826771455430847 82-83 0.49203600817632936 84-85 0.49363711705859525 86-87 0.4921694339165182 88-89 0.5000224917676318 90-91 0.5139940728474044 92-93 0.5208559680571154 94-95 0.5277178632668262 96-97 0.5186258521139593 98-99 0.521885252338572 100-101 0.5000796742277128 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2623182.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.54363027915412 #Duplication Level Percentage of deduplicated Percentage of total 1 67.8897462139131 28.203865164564096 2 14.807626705757098 12.303251383514038 3 6.2175720808871295 7.749015472870976 4 3.2428423360241556 5.388777722455041 5 1.9630323390325375 4.077574485939543 6 1.2546852141143179 3.1274507191131935 7 0.8870271660317874 2.579523004323331 8 0.5993385457357306 1.9918959164872878 9 0.4751612931135676 1.7765932575667351 >10 2.404228831340014 17.541738042954726 >50 0.15759219609049296 4.483742257329653 >100 0.09305156893605818 6.867577300532643 >500 0.005795648505896099 1.5946768418107689 >1k 0.0020238772560255964 1.4558314943934134 >5k 2.7598326218530856E-4 0.8584869361446786 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8926 0.34027375912155544 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7172 0.27340840246692755 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6365 0.24264423894339013 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4393 0.16746836475700122 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3210 0.12237046457317868 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.9060820026974872E-5 0.0 12-13 0.0 0.0 0.0 7.624328010789949E-5 0.0 14-15 0.0 0.0 0.0 9.530410013487437E-5 0.0 16-17 0.0 0.0 0.0 1.5248656021579898E-4 0.0 18-19 0.0 0.0 0.0 2.4779066035067335E-4 0.0 20-21 0.0 0.0 0.0 3.6215558051252256E-4 0.0 22-23 0.0 0.0 0.0 5.908854208362211E-4 0.0 24-25 0.0 0.0 0.0 0.0011436492016184924 0.0 26-27 0.0 0.0 0.0 0.0016582913423468138 0.0 28-29 0.0 0.0 0.0 0.005527637807822713 0.0 30-31 0.0 0.0 0.0 0.015744237342281246 0.0 32-33 0.0 0.0 0.0 0.0363299229714141 0.0 34-35 0.0 0.0 0.0 0.0684855263569207 0.0 36-37 0.0 0.0 0.0 0.12242764703325962 0.0 38-39 0.0 0.0 0.0 0.19512561461614175 0.0 40-41 0.0 0.0 0.0 0.2767249851516212 0.0 42-43 0.0 0.0 0.0 0.3632801688941141 0.0 44-45 0.0 0.0 0.0 0.47009700432528123 0.0 46-47 0.0 0.0 0.0 0.6021884871122172 0.0 48-49 0.0 0.0 0.0 0.7454496104349603 0.0 50-51 0.0 0.0 0.0 0.897288102769842 0.0 52-53 0.0 0.0 0.0 1.0512423461277183 1.9060820026974872E-5 54-55 0.0 0.0 0.0 1.2250960855937558 3.8121640053949745E-5 56-57 0.0 0.0 0.0 1.4361946673925026 3.8121640053949745E-5 58-59 0.0 0.0 0.0 1.6709096052046712 3.8121640053949745E-5 60-61 0.0 0.0 0.0 1.925981498805649 3.8121640053949745E-5 62-63 0.0 0.0 0.0 2.1859139015135054 3.8121640053949745E-5 64-65 0.0 0.0 0.0 2.4544236732335003 3.8121640053949745E-5 66-67 0.0 0.0 0.0 2.755679933759838 3.8121640053949745E-5 68-69 0.0 0.0 0.0 3.1080573135985228 3.8121640053949745E-5 70-71 0.0 0.0 0.0 3.5003099289336386 3.8121640053949745E-5 72-73 0.0 0.0 0.0 3.8947926602119107 3.8121640053949745E-5 74-75 0.0 0.0 0.0 4.31083317894069 3.8121640053949745E-5 76-77 0.0 0.0 0.0 4.761754998318835 3.8121640053949745E-5 78-79 0.0 0.0 0.0 5.2747388477048105 3.8121640053949745E-5 80-81 0.0 0.0 0.0 5.834497949436981 3.8121640053949745E-5 82-83 0.0 0.0 0.0 6.414614006957962 3.8121640053949745E-5 84-85 0.0 0.0 0.0 7.0070242933963405 3.8121640053949745E-5 86-87 0.0 0.0 0.0 7.62829266135556 3.8121640053949745E-5 88-89 0.0 0.0 0.0 8.288387919709727 3.8121640053949745E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10920 0.0 25.950052 1 TACATGG 11700 0.0 23.964952 2 ACATGGG 11335 0.0 23.650246 3 GTATCAA 18825 0.0 23.62605 1 GAGTACT 7970 0.0 22.244516 12-13 CATGGGG 5985 0.0 21.20857 4 GGTATCA 14180 0.0 20.452742 1 AGTACTT 8380 0.0 20.450033 12-13 TATCAAC 21590 0.0 20.379984 2 ATCAACG 21660 0.0 20.37972 3 CAACGCA 21650 0.0 20.301237 5 TCAACGC 21750 0.0 20.251839 4 AACGCAG 21980 0.0 20.02922 6 GTACTTT 9025 0.0 19.776077 14-15 GTATAGG 675 0.0 18.98657 1 CATGGGA 5560 0.0 18.826008 4 ACGCAGA 24080 0.0 18.06624 7 TTTGCGT 425 3.6379788E-12 17.830841 3 GTATATG 935 0.0 17.768183 1 CGCAGAG 24225 0.0 17.743155 8 >>END_MODULE