FastQCFastQC Report
Fri 27 May 2016
SRR939053_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939053_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2623182
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83430.3180488429701027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70150.26742330497845745No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT61340.2338381400909277No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45490.1734153406054174No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32740.12481024953663146No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG109000.024.210621
TACATGG114500.022.5453662
GAGTACT77100.022.43679812-13
TATAGCG2554.965841E-1022.3308355
ACATGGG111150.022.3283233
AGTACTT83900.020.0526612-13
GTACTTT87450.019.83578514-15
GTATCAA202000.019.596221
GGTATCA160100.019.5477471
CATGGGG58300.019.0463264
GTATAGG9550.018.4552971
CATGGGA58450.018.2667754
CGCGGAA5650.018.16676982-83
ATAGCGT1600.001199501217.7948866
TCGCGCT6650.017.48469260-61
ATCAACG226650.017.3565223
CAACGCA226850.017.341225
TATCAAC228400.017.3066432
TCAACGC227700.017.2764854
AACGCAG229300.017.1973236
Produced by FastQC (version 0.11.5)