##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939053_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2623182 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20064410323035 33.0 31.0 34.0 30.0 34.0 2 32.4405024127186 34.0 31.0 34.0 31.0 34.0 3 32.5284776275531 34.0 31.0 34.0 31.0 34.0 4 36.07363728479381 37.0 35.0 37.0 35.0 37.0 5 35.99567738723428 37.0 35.0 37.0 35.0 37.0 6 36.036498801836856 37.0 35.0 37.0 35.0 37.0 7 35.969676141419086 37.0 35.0 37.0 35.0 37.0 8 35.94620693493627 37.0 35.0 37.0 35.0 37.0 9 37.622282403584656 39.0 37.0 39.0 35.0 39.0 10-11 37.538497710033084 39.0 37.0 39.0 35.0 39.0 12-13 37.44711098963015 39.0 37.0 39.0 34.0 39.0 14-15 38.769342348338775 40.0 38.0 41.0 34.5 41.0 16-17 38.8549991956334 40.0 38.0 41.0 35.0 41.0 18-19 38.88802473484493 40.0 38.0 41.0 35.0 41.0 20-21 38.860832568994454 40.0 38.0 41.0 34.5 41.0 22-23 38.78775186014543 40.0 38.0 41.0 34.0 41.0 24-25 38.707029477939386 40.0 38.0 41.0 34.0 41.0 26-27 38.54917291289739 40.0 38.0 41.0 34.0 41.0 28-29 38.39159825738359 40.0 38.0 41.0 34.0 41.0 30-31 38.2010350406491 40.0 38.0 41.0 33.5 41.0 32-33 38.12399825860348 40.0 38.0 41.0 33.0 41.0 34-35 38.02878736587854 40.0 37.5 41.0 33.0 41.0 36-37 37.90407127679284 40.0 37.0 41.0 33.0 41.0 38-39 37.76376915517109 40.0 37.0 41.0 33.0 41.0 40-41 37.59032198299622 40.0 37.0 41.0 33.0 41.0 42-43 37.40541029939973 40.0 37.0 41.0 32.0 41.0 44-45 37.16095680741939 40.0 36.5 41.0 31.0 41.0 46-47 36.843276028884006 40.0 36.0 41.0 31.0 41.0 48-49 36.52145733692897 39.0 36.0 41.0 30.0 41.0 50-51 36.25460509411852 39.0 35.0 41.0 30.0 41.0 52-53 35.982277440147115 39.0 35.0 40.0 29.0 41.0 54-55 35.67126623314738 38.5 35.0 40.0 28.0 41.0 56-57 35.538902180634054 38.0 35.0 40.0 28.0 41.0 58-59 35.24954902099816 38.0 34.5 40.0 28.0 41.0 60-61 34.87777287279343 38.0 34.0 40.0 27.0 41.0 62-63 34.456797126543265 37.0 34.0 40.0 26.0 41.0 64-65 34.04125828859759 36.5 33.5 39.0 26.0 41.0 66-67 33.58921798030026 36.0 33.0 39.0 25.5 40.0 68-69 33.107315847699475 35.5 32.5 38.5 24.5 40.0 70-71 32.58216852662149 35.0 32.0 38.0 23.0 40.0 72-73 32.07959207557844 35.0 31.0 37.0 22.0 39.0 74-75 31.561700636860117 35.0 31.0 36.5 20.5 39.0 76-77 29.654652822411865 32.5 28.5 35.0 18.0 37.0 78-79 30.635439897041074 34.0 30.0 35.0 19.0 37.0 80-81 30.622290599737266 34.0 31.0 35.0 18.0 37.0 82-83 30.234559401520748 34.0 30.0 35.0 16.5 36.5 84-85 29.80457265260283 34.0 30.0 35.0 9.5 36.0 86-87 29.128315343731394 34.0 29.0 35.0 4.5 36.0 88-89 28.466516238675013 33.0 28.5 35.0 2.0 35.0 90-91 27.89644504270005 33.0 27.0 35.0 2.0 35.0 92-93 27.50327312401503 33.0 26.5 35.0 2.0 35.0 94-95 26.963097299386774 32.5 25.5 34.0 2.0 35.0 96-97 26.13347205798149 32.0 24.5 34.0 2.0 35.0 98-99 24.943930691808653 32.0 19.0 34.0 2.0 35.0 100-101 19.45021790329455 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07826886320020066 1101 2 0.09501453842567997 1101 3 0.1582606985621453 1101 4 0.04793252954844718 1101 5 0.1009489861595867 1101 6 0.07347518604707659 1101 7 0.08683756532750664 1101 8 0.08807823734593256 1101 9 0.08087785168363126 1101 10-11 0.12006727817276186 1101 12-13 0.1050752927475358 1101 14-15 0.16096675527980153 1101 16-17 0.1971526225775122 1101 18-19 0.11456656517691499 1101 20-21 0.2453026743168678 1101 22-23 0.18411039174238653 1101 24-25 0.1223666185079253 1101 26-27 0.02643881492794975 1101 28-29 0.11431451745222176 1101 30-31 0.045095272572154954 1101 32-33 0.14208719842832096 1101 34-35 0.18476365777801362 1101 36-37 0.1320965389574269 1101 38-39 0.1568933860713102 1101 40-41 0.2360631840534424 1101 42-43 0.21292142362470656 1101 44-45 0.14025686052855946 1101 46-47 0.18398598042109882 1101 48-49 0.045020368264552246 1101 50-51 0.03961260429844771 1101 52-53 0.08735526150667994 1101 54-55 0.09818552095216404 1101 56-57 -0.05677932807327579 1101 58-59 0.09414437213572313 1101 60-61 0.04167969680791117 1101 62-63 0.19414541649255312 1101 64-65 0.25029422365653886 1101 66-67 0.19612368922080492 1101 68-69 0.3857569204122129 1101 70-71 0.2511228511911838 1101 72-73 -0.05152548418685399 1101 74-75 -0.10587879084562601 1101 76-77 -0.1743170346340328 1101 78-79 0.1360628504828476 1101 80-81 0.22775134147185483 1101 82-83 0.027187840684472775 1101 84-85 0.0870785310130735 1101 86-87 0.26155324648721745 1101 88-89 0.4687613518654139 1101 90-91 0.22921128845319672 1101 92-93 0.08223115729664343 1101 94-95 0.06650393658548737 1101 96-97 0.20107046928169225 1101 98-99 0.5446756617257904 1101 100-101 0.8524938273202807 1102 1 0.10568062386280985 1102 2 0.13670440336021272 1102 3 0.13609776080224378 1102 4 0.09615055889855029 1102 5 0.04524736743600499 1102 6 0.08474267066748808 1102 7 0.1415846182882916 1102 8 0.08403658356719035 1102 9 0.09681763929060594 1102 10-11 0.21303562542505716 1102 12-13 0.19590209619806132 1102 14-15 0.2679013189805346 1102 16-17 0.3044373082839442 1102 18-19 0.19109421407543437 1102 20-21 0.1528004404095924 1102 22-23 0.21695913903592157 1102 24-25 0.2388270783491464 1102 26-27 0.2233044711811658 1102 28-29 0.17849725106736258 1102 30-31 0.1715932792395023 1102 32-33 0.2659457577299662 1102 34-35 0.14487423618816564 1102 36-37 0.22298864123600737 1102 38-39 0.2761140101906392 1102 40-41 0.12569871348947004 1102 42-43 0.228892571853045 1102 44-45 0.12548343027801678 1102 46-47 0.25575068124936706 1102 48-49 0.42110080611038114 1102 50-51 0.4468642109932972 1102 52-53 0.357665774617999 1102 54-55 0.36981275056346163 1102 56-57 0.3504563835121104 1102 58-59 0.3282909336369073 1102 60-61 0.19261321229060968 1102 62-63 0.3726470661471595 1102 64-65 0.3379806689945468 1102 66-67 0.1603395018475311 1102 68-69 0.21845573815974006 1102 70-71 0.35961169883871236 1102 72-73 0.4182600607146867 1102 74-75 0.3226323099551429 1102 76-77 0.12206704276226077 1102 78-79 0.31560498541085025 1102 80-81 0.30821496315106955 1102 82-83 0.2697111248718471 1102 84-85 0.359198509017677 1102 86-87 0.5447546933717753 1102 88-89 0.41459210761343357 1102 90-91 0.3781871278868181 1102 92-93 0.5166797258775979 1102 94-95 0.5861640390015417 1102 96-97 0.7417434768082423 1102 98-99 0.8656911444216036 1102 100-101 0.9156755986369092 1103 1 0.057744242859655515 1103 2 0.0540300656878685 1103 3 0.059065610699320814 1103 4 0.06656609638726252 1103 5 0.14181244510864133 1103 6 0.11054716368509077 1103 7 0.15848156321402485 1103 8 0.1653003743471757 1103 9 0.14535007816690637 1103 10-11 0.18178516264720201 1103 12-13 0.16482563789249838 1103 14-15 0.2907144159639614 1103 16-17 0.10810287909265526 1103 18-19 0.2082891879377371 1103 20-21 0.10776475098165861 1103 22-23 0.2103950979856748 1103 24-25 0.16632274674844894 1103 26-27 0.18239490908008094 1103 28-29 0.1582386878259996 1103 30-31 0.16858707431406827 1103 32-33 0.2660494199687733 1103 34-35 0.2811530579927748 1103 36-37 0.3269766756518706 1103 38-39 0.19104803658008507 1103 40-41 0.39626456122444864 1103 42-43 0.37813102462008885 1103 44-45 0.26313762785824935 1103 46-47 0.36046435133688703 1103 48-49 0.24893818967547787 1103 50-51 0.359969339312336 1103 52-53 0.24793374150531378 1103 54-55 0.3926536142179984 1103 56-57 0.3977874510850796 1103 58-59 0.4090808398966246 1103 60-61 0.31343895606717354 1103 62-63 0.37569954885777435 1103 64-65 0.25986006538413875 1103 66-67 0.18330624107776572 1103 68-69 0.3042066229909963 1103 70-71 0.25945916725874696 1103 72-73 0.23067398403424022 1103 74-75 0.1832084498695501 1103 76-77 -0.017416052888368228 1103 78-79 0.37632699290414706 1103 80-81 0.379706736811972 1103 82-83 0.3604951816195161 1103 84-85 0.4149151112656675 1103 86-87 0.4751436489006764 1103 88-89 0.42018986850907325 1103 90-91 0.5154174209547762 1103 92-93 0.7707540220850397 1103 94-95 0.9439246384654609 1103 96-97 0.487756182836943 1103 98-99 0.7632506391422353 1103 100-101 0.47413739910190955 1104 1 0.031727982697056234 1104 2 0.08033396907778467 1104 3 0.05821932153467202 1104 4 0.011355237261128082 1104 5 0.008570634620362227 1104 6 0.08018653990341562 1104 7 0.05733574473829606 1104 8 0.03620799570408906 1104 9 0.06908539885344567 1104 10-11 0.1061439084276472 1104 12-13 0.12225503455488251 1104 14-15 0.08590055203584512 1104 16-17 0.13110661132295576 1104 18-19 0.09291810440234372 1104 20-21 0.10317672264523736 1104 22-23 0.03545928283805466 1104 24-25 0.12912762479723483 1104 26-27 0.13696926842016666 1104 28-29 0.09758578524434114 1104 30-31 0.03574820206569029 1104 32-33 0.1402459207057163 1104 34-35 0.11857282692730564 1104 36-37 0.1950227223404113 1104 38-39 0.04845247246655049 1104 40-41 0.09689155214348233 1104 42-43 -0.025596451251161056 1104 44-45 0.1267596741664292 1104 46-47 0.20190637102024311 1104 48-49 0.0492656512834273 1104 50-51 0.010549380200465919 1104 52-53 0.04527482456527565 1104 54-55 0.24415126077341398 1104 56-57 0.21567184703430087 1104 58-59 0.29527396498577474 1104 60-61 0.3035218020710744 1104 62-63 0.34576325826679977 1104 64-65 0.3249797582477356 1104 66-67 0.25271918740020993 1104 68-69 0.2520718823453407 1104 70-71 0.2503532384801588 1104 72-73 0.35262876937157017 1104 74-75 0.2683805603625835 1104 76-77 0.14093867539575555 1104 78-79 0.28879787199890217 1104 80-81 0.15316511174659908 1104 82-83 0.20479045571260812 1104 84-85 0.3492557901987716 1104 86-87 0.5783564573445474 1104 88-89 0.5224149433914 1104 90-91 0.4296622980526692 1104 92-93 0.4067801476971731 1104 94-95 0.6490511063968079 1104 96-97 0.4953852657067195 1104 98-99 0.6894125038310577 1104 100-101 0.548070623083273 1105 1 0.17251688603472815 1105 2 0.1158608552137963 1105 3 0.14676676296008395 1105 4 0.1175708738819381 1105 5 0.09274180702471568 1105 6 0.11218469198061598 1105 7 0.15720114295424992 1105 8 0.08547909282270183 1105 9 0.15769614874508164 1105 10-11 0.2043681344673871 1105 12-13 0.18923966941902393 1105 14-15 0.1234715601803913 1105 16-17 0.1010473523171882 1105 18-19 0.12911457737931897 1105 20-21 0.16777417205549483 1105 22-23 0.08459292643990324 1105 24-25 0.06537599930959459 1105 26-27 0.14378348373739414 1105 28-29 0.18131180217222465 1105 30-31 0.25277646844034507 1105 32-33 0.27938253010958647 1105 34-35 0.3618723957453156 1105 36-37 0.29530418835916095 1105 38-39 0.34359454579030313 1105 40-41 0.422781007576603 1105 42-43 0.4486090560150515 1105 44-45 0.4176635023324238 1105 46-47 0.38708534576529985 1105 48-49 0.49108944842256363 1105 50-51 0.41104900377175113 1105 52-53 0.48145361679943477 1105 54-55 0.5163651179130184 1105 56-57 0.5862074594724191 1105 58-59 0.47838828524675847 1105 60-61 0.4958788732275039 1105 62-63 0.4323966166953781 1105 64-65 0.31042080722910015 1105 66-67 0.43998762670393177 1105 68-69 0.5450372280894129 1105 70-71 0.6103850638172403 1105 72-73 0.5840109471828043 1105 74-75 0.6793853969012638 1105 76-77 0.4159785996537373 1105 78-79 0.522166566817873 1105 80-81 0.566195248857909 1105 82-83 0.505395878567537 1105 84-85 0.5366434319298854 1105 86-87 0.5406080714252646 1105 88-89 0.8675293549078376 1105 90-91 0.5859700456401029 1105 92-93 0.7857346454500096 1105 94-95 0.8308564813426251 1105 96-97 0.7985538259239071 1105 98-99 1.0435757484184691 1105 100-101 0.8405833988458262 1106 1 -0.08321171028539709 1106 2 -0.07701252215028376 1106 3 -0.009269713553045733 1106 4 -0.053063622387995224 1106 5 -0.004011602221744681 1106 6 -2.1367939483241116E-4 1106 7 0.06142017456286197 1106 8 0.0477759781602316 1106 9 0.013082109379759288 1106 10-11 0.08411814088378122 1106 12-13 0.0012846398180457186 1106 14-15 -0.08574144357818625 1106 16-17 -0.20679086674721958 1106 18-19 -0.1612348561239756 1106 20-21 -0.06753325542494082 1106 22-23 -0.07458731803914276 1106 24-25 0.022206023920041673 1106 26-27 -0.11392163070264161 1106 28-29 -0.18040215335214782 1106 30-31 -0.04916133740798756 1106 32-33 0.005075964565357083 1106 34-35 -0.11524022131830947 1106 36-37 -0.038063900466603684 1106 38-39 -0.03723831700713731 1106 40-41 0.03128025828383585 1106 42-43 -0.025226385461685652 1106 44-45 0.047469652236607374 1106 46-47 -0.008016874593792522 1106 48-49 0.22899426970447934 1106 50-51 0.1647708714285372 1106 52-53 0.20197712719684802 1106 54-55 0.19287658972078248 1106 56-57 0.12384070668342417 1106 58-59 -0.03231649554054883 1106 60-61 0.07556127575528393 1106 62-63 0.15864703019662585 1106 64-65 0.11127361789685608 1106 66-67 0.04311118301424699 1106 68-69 0.10549841743306132 1106 70-71 0.2734206726906834 1106 72-73 0.09208874744174977 1106 74-75 0.18681531913451366 1106 76-77 -0.06288533776213612 1106 78-79 0.1684579597182001 1106 80-81 0.08891072578168746 1106 82-83 0.13125701273015267 1106 84-85 0.21985611914614012 1106 86-87 0.37262729067788314 1106 88-89 0.49010957058438365 1106 90-91 0.6296348857719671 1106 92-93 0.7621529547147183 1106 94-95 0.8500379485020702 1106 96-97 0.8316928314961949 1106 98-99 0.7859996748836906 1106 100-101 1.0693562590481847 1107 1 0.0773590506582238 1107 2 0.10745882004014362 1107 3 0.08414761313746766 1107 4 0.06432747367740888 1107 5 0.039796149864784525 1107 6 0.07948819209214264 1107 7 0.14776752808014493 1107 8 0.1683447696778586 1107 9 0.011965657753123082 1107 10-11 0.1341289194990125 1107 12-13 0.10219243874497153 1107 14-15 0.16520135258089397 1107 16-17 0.14100909812247409 1107 18-19 0.11169897648545657 1107 20-21 0.16431409276957964 1107 22-23 0.17556105425689594 1107 24-25 0.07848037560629706 1107 26-27 0.18138248592996575 1107 28-29 0.22680993785888148 1107 30-31 0.15748001947329726 1107 32-33 0.16306784201383806 1107 34-35 0.21481494922333866 1107 36-37 0.33963607611319446 1107 38-39 0.20970907270501016 1107 40-41 0.16446734843031408 1107 42-43 0.19208580049470925 1107 44-45 0.1408224403050724 1107 46-47 0.15703965325495517 1107 48-49 0.17349704286918666 1107 50-51 0.011828974818079985 1107 52-53 0.016370038327835346 1107 54-55 0.05140726486130376 1107 56-57 0.14015234439420254 1107 58-59 0.1637099213816171 1107 60-61 0.2497703116946397 1107 62-63 0.2259880921962818 1107 64-65 0.2176172987008087 1107 66-67 0.2456313829375958 1107 68-69 0.3468555648525822 1107 70-71 0.4198282001561964 1107 72-73 0.326159684036881 1107 74-75 0.24456008684870412 1107 76-77 0.13244778798305035 1107 78-79 0.47939572585684687 1107 80-81 0.4759614837445554 1107 82-83 0.45475851907489684 1107 84-85 0.4062456556390721 1107 86-87 0.453941962369246 1107 88-89 0.40631249065762276 1107 90-91 0.5087267674786631 1107 92-93 0.7812350957469079 1107 94-95 0.8024896177236691 1107 96-97 0.9430538911538306 1107 98-99 0.88767727984877 1107 100-101 0.7161723093377432 1108 1 0.06137262531292009 1108 2 0.05473109891087802 1108 3 0.062350342236001666 1108 4 0.05776610634709556 1108 5 -0.010342705682155895 1108 6 0.03156384311279936 1108 7 0.0718657869089867 1108 8 0.13418266484553243 1108 9 0.09479476245295615 1108 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-1.6037091354408552 2316 98-99 -1.6967931497380988 2316 100-101 -1.0131648535684583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 20.0 5 51.0 6 115.0 7 320.0 8 838.0 9 1435.0 10 2374.0 11 3743.0 12 5530.0 13 7591.0 14 9136.0 15 11977.0 16 15287.0 17 21869.0 18 21506.0 19 15287.0 20 14499.0 21 16090.0 22 17232.0 23 19189.0 24 22064.0 25 26423.0 26 31134.0 27 38482.0 28 46247.0 29 56623.0 30 68576.0 31 82767.0 32 103110.0 33 132119.0 34 179739.0 35 258955.0 36 410295.0 37 598243.0 38 367028.0 39 17280.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.742658377942256 19.497295141512286 14.5897778012528 23.170268679292658 2 15.646493457182917 21.142833398521336 39.042811364213385 24.167861780082358 3 19.13260307519646 24.768620705692552 30.543324862704914 25.555451356406074 4 13.19237475706985 16.964320432207906 36.60821094380794 33.235093866914305 5 13.745786605733038 37.63463610226054 34.0202090438254 14.599368248181028 6 31.994272604798297 35.88283237686139 17.216304472964513 14.906590545375806 7 28.103844872372562 33.221865657815584 20.860504532281787 17.81378493753007 8 26.157544539418158 34.86269728901769 19.78227206499587 19.19748610656828 9 26.372016886361678 16.65725062157334 20.656515636353102 36.31421685571188 10-11 25.239537325278995 26.258433459820935 27.151890337765355 21.350138877134718 12-13 26.10893944834937 23.822784694314006 27.754269433077845 22.314006424258782 14-15 22.92641039203633 25.000338332070545 26.32446522089272 25.748786055000405 16-17 22.30004959755832 28.435965206226342 26.79299366821025 22.470991528005086 18-19 22.18222400547429 27.11476275387913 28.807388763034076 21.89562447761251 20-21 22.996002564823943 26.12735220049543 28.662060047682548 22.214585186998082 22-23 22.806652378675974 26.1312215469609 28.537955048486914 22.524171025876207 24-25 22.780071218875097 26.34290199570674 28.585293961796765 22.291732823621395 26-27 22.62218176245491 26.313118952478327 28.745069918900022 22.31962936616674 28-29 22.22941450497907 26.711432908582022 28.766494280610345 22.29265830582857 30-31 22.450767807952328 26.36873842531704 28.653349252930223 22.527144513800415 32-33 22.349821705089468 26.581304690257863 28.50963066992683 22.55924293472584 34-35 22.8768940040212 26.400428236541973 28.32732110630061 22.39535665313622 36-37 22.87822181753291 26.185832467776105 28.399523676612564 22.536422038078417 38-39 22.67344895167069 26.377557916079137 28.615109583377098 22.333883548873075 40-41 22.661971847094566 26.39842884172635 28.56740650994479 22.3721928012343 42-43 22.464482656541556 26.63417141003248 28.076404963002034 22.824940970423928 44-45 22.642157151464588 26.498864244802373 27.986372843737733 22.872605759995306 46-47 22.835949173389967 26.723903118898683 27.595354217738304 22.844793489973043 48-49 22.92353313546339 26.723669119422848 27.52430470242596 22.828493042687796 50-51 22.490619163781794 26.932330271004478 27.659161616473522 22.917888948740206 52-53 22.959234242991908 26.953085984884005 27.307617237385738 22.78006253473834 54-55 22.486011264182203 26.70072072772686 27.445484148640848 23.367783859450086 56-57 22.636038978614522 27.100826400913093 27.233508769120863 23.029625851351526 58-59 23.018511448308253 26.60058353272703 27.34066405772731 23.04024096123741 60-61 22.820779676265847 26.571856629840575 27.82552589644472 22.78183779744886 62-63 22.60168000721272 26.67454144235723 27.776234887645302 22.947543662784746 64-65 22.380849814727274 26.823487701855775 27.60723707284344 23.188425410573508 66-67 22.42571430314176 26.906320574461823 27.20480146512773 23.463163657268684 68-69 22.231553794459565 26.719483026662203 27.637475916029576 23.41148726284866 70-71 22.43468707244149 26.733059390914608 27.35915086236967 23.47310267427423 72-73 22.478234449611197 26.49854260970074 27.379457468067407 23.643765472620657 74-75 22.202024106600305 27.014309338810648 27.10921316172496 23.674453392864088 76-77 22.365019530810297 26.545914098013114 27.29391150789608 23.795154863280512 78-79 22.24693862846438 26.747243421782485 27.277496112407928 23.728321837345206 80-81 22.262095461452425 26.844292448766264 27.248001848142746 23.645610241638565 82-83 22.212774409095516 26.629547625746135 27.409249529769568 23.748428435388778 84-85 22.117588710178534 26.515442915213118 27.13635031109224 24.23061806351611 86-87 22.06859000114852 26.352154965676 27.44475745785289 24.134497575322587 88-89 22.141265574540025 26.230585608256824 27.24965152735451 24.378497289848646 90-91 21.99504205451444 26.27915854614133 27.361238280850102 24.364561118494134 92-93 22.03282120722085 26.319980847688036 27.21852696457966 24.428670980511455 94-95 21.982242001619422 25.99441283705582 27.238772684506756 24.784572476818 96-97 21.654101774104884 25.89989943511354 27.402730729320346 25.043268061461234 98-99 21.61304476776678 25.989999931381046 27.13523118106178 25.26172411979039 100-101 21.71963692783922 25.211900105597422 27.870591688682016 25.19787127788134 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 768.0 1 536.5 2 351.5 3 412.0 4 380.0 5 404.0 6 632.5 7 1205.0 8 1933.0 9 2412.0 10 2459.5 11 2422.5 12 2629.0 13 2837.0 14 3037.5 15 3230.5 16 3340.0 17 3507.0 18 3874.5 19 4180.0 20 4552.5 21 5301.5 22 6454.0 23 7832.0 24 9205.5 25 10943.5 26 13683.0 27 16860.5 28 19509.0 29 22481.5 30 26160.5 31 30886.0 32 35675.0 33 39500.5 34 44656.5 35 49310.0 36 53541.0 37 58821.0 38 62551.5 39 63882.0 40 65548.0 41 70643.5 42 75203.5 43 80169.0 44 86671.0 45 90769.5 46 93619.5 47 101019.5 48 118067.5 49 119295.0 50 106595.5 51 102029.5 52 98481.5 53 97898.5 54 97022.5 55 91835.0 56 84453.5 57 76275.0 58 69181.0 59 61538.5 60 52131.0 61 41225.5 62 32562.5 63 26146.0 64 20000.5 65 14706.5 66 9801.0 67 7108.5 68 4772.0 69 2456.0 70 1575.5 71 964.0 72 550.0 73 330.0 74 217.5 75 148.0 76 90.5 77 42.5 78 15.0 79 8.5 80 9.0 81 7.0 82 8.5 83 6.5 84 2.0 85 3.0 86 2.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3993623012051775 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 7.4337198105202E-4 16-17 0.0026303931637225325 18-19 3.6215558051252256E-4 20-21 0.0 22-23 0.0 24-25 3.8121640053949745E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01267544531793829 36-37 0.02264425419204615 38-39 0.010311903634593406 40-41 0.005680124368038512 42-43 0.005051117307148341 44-45 0.0014295615020231156 46-47 0.0010864667415375678 48-49 0.002992548744235055 50-51 5.718246008092462E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.196152611599196E-4 60-61 0.0016011088822658894 62-63 0.0014676831420770652 64-65 8.386760811868943E-4 66-67 0.0025160282435606834 68-69 0.0025922715236685826 70-71 1.1436492016184923E-4 72-73 0.0 74-75 0.0 76-77 0.09343613977223084 78-79 0.0035453125250173266 80-81 3.0497312043159796E-4 82-83 0.0 84-85 1.9060820026974872E-4 86-87 0.09229249057061234 88-89 0.43563504171651074 90-91 0.38901227593053017 92-93 0.0 94-95 9.911626414026934E-4 96-97 0.0 98-99 0.0 100-101 4.574596806473969E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2623182.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.21826304414736 #Duplication Level Percentage of deduplicated Percentage of total 1 66.46866397069063 26.73254211766276 2 15.476372007042965 12.448656006966656 3 6.373626849269409 7.690086035074718 4 3.4536869421870913 5.556051596520696 5 2.0058925665707084 4.033675744032031 6 1.325879527649924 3.19947429647247 7 0.887117521057518 2.4974828069074233 8 0.6652782883239369 2.1405069757897763 9 0.5019818706831662 1.8169955026675868 >10 2.57059846034309 18.200970639531644 >50 0.1639580179060356 4.577642149528528 >100 0.09804315249589386 7.065740853861103 >500 0.006533584880883192 1.7465027645031008 >1k 0.002083171991004548 1.4752028431240962 >5k 2.8406890786425654E-4 0.8184696673573864 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8343 0.3180488429701027 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7015 0.26742330497845745 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6134 0.2338381400909277 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4549 0.1734153406054174 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3274 0.12481024953663146 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.8121640053949745E-5 0.0 3 0.0 0.0 0.0 3.8121640053949745E-5 0.0 4 0.0 0.0 0.0 3.8121640053949745E-5 0.0 5 0.0 0.0 0.0 3.8121640053949745E-5 0.0 6 0.0 0.0 0.0 3.8121640053949745E-5 0.0 7 0.0 0.0 0.0 3.8121640053949745E-5 0.0 8 0.0 0.0 0.0 3.8121640053949745E-5 0.0 9 0.0 0.0 0.0 7.624328010789949E-5 0.0 10-11 0.0 0.0 0.0 7.624328010789949E-5 0.0 12-13 0.0 0.0 0.0 1.9060820026974872E-4 0.0 14-15 0.0 0.0 0.0 2.096690202967236E-4 0.0 16-17 0.0 0.0 0.0 2.859123004046231E-4 0.0 18-19 0.0 0.0 0.0 4.002772205664723E-4 0.0 20-21 0.0 0.0 0.0 5.146421407283216E-4 0.0 22-23 0.0 0.0 0.0 8.196152611599195E-4 0.0 24-25 0.0 0.0 0.0 0.0014295615020231156 0.0 26-27 0.0 0.0 0.0 0.0020013861028323615 0.0 28-29 0.0 0.0 0.0 0.005927915028389186 0.0 30-31 0.0 0.0 0.0 0.016487609323333263 0.0 32-33 0.0 0.0 0.0 0.0375498154531405 0.0 34-35 0.0 0.0 0.0 0.07033442589953728 0.0 36-37 0.0 0.0 0.0 0.12473400625652356 0.0 38-39 0.0 0.0 0.0 0.19827064992059262 0.0 40-41 0.0 0.0 0.0 0.2811280345778524 0.0 42-43 0.0 0.0 0.0 0.36859813768164007 0.0 44-45 0.0 0.0 0.0 0.476368014114156 0.0 46-47 0.0 0.0 0.0 0.6097175110228723 0.0 48-49 0.0 0.0 0.0 0.7546178648679353 0.0 50-51 0.0 0.0 0.0 0.9077524929646513 0.0 52-53 0.0 0.0 0.0 1.0632697235647393 0.0 54-55 0.0 0.0 0.0 1.2393345181539064 0.0 56-57 0.0 0.0 0.0 1.4521295129350538 0.0 58-59 0.0 0.0 0.0 1.6888648976700815 0.0 60-61 0.0 0.0 0.0 1.9456332042534603 0.0 62-63 0.0 0.0 0.0 2.20724295912369 0.0 64-65 0.0 0.0 0.0 2.4782878199072726 0.0 66-67 0.0 0.0 0.0 2.7835277918192487 0.0 68-69 0.0 0.0 0.0 3.1394123625428962 0.0 70-71 0.0 0.0 0.0 3.5351149863028946 0.0 72-73 0.0 0.0 0.0 3.9346869565283686 3.8121640053949745E-5 74-75 0.0 0.0 0.0 4.355492680263893 3.8121640053949745E-5 76-77 0.0 0.0 0.0 4.809559534946489 3.8121640053949745E-5 78-79 0.0 0.0 0.0 5.3266033389982095 3.8121640053949745E-5 80-81 0.0 0.0 0.0 5.888706921593698 3.8121640053949745E-5 82-83 0.0 0.0 0.0 6.472730447220208 3.8121640053949745E-5 84-85 0.0 0.0 0.0 7.069105384224198 3.8121640053949745E-5 86-87 0.0 0.0 0.0 7.693938125528462 3.8121640053949745E-5 88-89 0.0 0.0 0.0 8.35975162989072 3.8121640053949745E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10900 0.0 24.21062 1 TACATGG 11450 0.0 22.545366 2 GAGTACT 7710 0.0 22.436798 12-13 TATAGCG 255 4.965841E-10 22.330835 5 ACATGGG 11115 0.0 22.328323 3 AGTACTT 8390 0.0 20.05266 12-13 GTACTTT 8745 0.0 19.835785 14-15 GTATCAA 20200 0.0 19.59622 1 GGTATCA 16010 0.0 19.547747 1 CATGGGG 5830 0.0 19.046326 4 GTATAGG 955 0.0 18.455297 1 CATGGGA 5845 0.0 18.266775 4 CGCGGAA 565 0.0 18.166769 82-83 ATAGCGT 160 0.0011995012 17.794886 6 TCGCGCT 665 0.0 17.484692 60-61 ATCAACG 22665 0.0 17.356522 3 CAACGCA 22685 0.0 17.34122 5 TATCAAC 22840 0.0 17.306643 2 TCAACGC 22770 0.0 17.276485 4 AACGCAG 22930 0.0 17.197323 6 >>END_MODULE