FastQCFastQC Report
Fri 27 May 2016
SRR939052_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939052_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2642499
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90500.34247884294374376No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72890.2758373796924805No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT62760.23750245506242387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44880.16983923172724003No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32920.12457904430616626No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGT4950.028.6840153
GTACATG108600.025.275351
TACATGG113750.023.757932
ACATGGG112250.022.9791553
GTATCAA201800.021.7069721
GAGTACT82700.021.6612912-13
CATGGGG61500.020.1627644
AGTACTT87400.019.46755212-13
CCGTCTA1951.0662272E-519.4168729
GGTATCA155950.019.3339311
GTACTTT92950.019.14585114-15
TATCAAC230750.018.8083862
ATCAACG230350.018.7383143
CAACGCA231100.018.7184625
TCAACGC232100.018.6174224
AACGCAG233800.018.5001936
CTTTGCG7950.018.4551892
CATGGGA56150.018.3751374
TATAGGG3451.1386874E-917.8339752
AGAGTAC168650.017.55354710-11
Produced by FastQC (version 0.11.5)