##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939052_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2642499 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.86766693194586 33.0 31.0 34.0 30.0 34.0 2 31.92621529847315 33.0 31.0 34.0 30.0 34.0 3 31.978585043930007 34.0 31.0 34.0 30.0 34.0 4 35.51891864481311 37.0 35.0 37.0 33.0 37.0 5 35.5570711663467 37.0 35.0 37.0 33.0 37.0 6 35.63808387439314 37.0 35.0 37.0 33.0 37.0 7 35.550039186391366 37.0 35.0 37.0 33.0 37.0 8 35.534870968730736 37.0 35.0 37.0 33.0 37.0 9 37.14805417144907 39.0 37.0 39.0 33.0 39.0 10-11 37.04179660995142 39.0 37.0 39.0 33.0 39.0 12-13 36.92010063201538 39.0 37.0 39.0 33.0 39.0 14-15 38.14917886440071 40.0 38.0 41.0 33.0 41.0 16-17 38.08141630327958 40.0 38.0 41.0 33.0 41.0 18-19 38.01545052618752 40.0 38.0 41.0 33.0 41.0 20-21 37.905571203622024 40.0 38.0 41.0 32.5 41.0 22-23 37.98056763692247 40.0 38.0 41.0 33.0 41.0 24-25 37.90780696605751 40.0 38.0 41.0 33.0 41.0 26-27 37.83128337986126 40.0 38.0 41.0 32.0 41.0 28-29 37.70623300141268 40.0 37.5 41.0 32.0 41.0 30-31 37.52695005750239 40.0 37.0 41.0 32.0 41.0 32-33 37.34529208904147 40.0 37.0 41.0 31.5 41.0 34-35 37.03376500804731 40.0 36.0 41.0 30.5 41.0 36-37 36.77154163539892 40.0 36.0 41.0 30.0 41.0 38-39 36.57497391673563 39.0 36.0 41.0 30.0 41.0 40-41 36.4161358244601 39.0 35.5 41.0 30.0 41.0 42-43 36.20682921734313 39.0 35.0 41.0 30.0 41.0 44-45 36.14215918340934 39.0 35.0 41.0 29.0 41.0 46-47 35.911083410059945 39.0 35.0 41.0 28.0 41.0 48-49 35.724510207950885 39.0 35.0 41.0 27.0 41.0 50-51 34.54338904196369 38.0 34.0 40.0 25.0 40.5 52-53 34.6724363944887 38.0 34.5 39.5 26.0 40.5 54-55 35.15825947332431 39.0 35.0 40.5 26.0 41.0 56-57 35.057379775735015 39.0 35.0 41.0 26.0 41.0 58-59 34.85208376616225 38.0 34.5 40.0 26.0 41.0 60-61 34.57482235565652 38.0 34.0 40.0 25.5 41.0 62-63 34.214597242988546 37.5 34.0 40.0 24.5 41.0 64-65 33.79756283729909 37.0 33.5 40.0 23.0 41.0 66-67 33.41936610004393 36.0 33.0 39.0 22.5 41.0 68-69 33.025545137387 36.0 33.0 39.0 22.0 41.0 70-71 32.60576011570865 35.0 32.5 38.5 20.5 40.0 72-73 32.17017925077739 35.0 32.0 37.5 20.0 40.0 74-75 31.71118664567139 35.0 31.5 37.0 18.0 39.0 76-77 31.307816767385724 35.0 31.0 36.5 17.0 39.0 78-79 30.891955872074124 35.0 31.0 36.0 12.5 38.5 80-81 30.459582009302558 34.5 31.0 35.5 7.5 37.0 82-83 30.095005523180898 34.0 30.5 35.0 6.5 37.0 84-85 29.752798771163206 34.0 30.0 35.0 2.0 36.5 86-87 29.364946779544667 34.0 30.0 35.0 2.0 36.0 88-89 28.966431775376265 34.0 29.0 35.0 2.0 36.0 90-91 28.549548741551085 34.0 29.0 35.0 2.0 35.0 92-93 28.133857761157145 34.0 29.0 35.0 2.0 35.0 94-95 27.663557488574263 34.0 27.0 35.0 2.0 35.0 96-97 27.081123020292534 33.5 26.5 35.0 2.0 35.0 98-99 26.388893430044817 33.0 25.0 35.0 2.0 35.0 100-101 23.795932562320743 31.0 12.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29807567715853267 1101 2 0.3158097379617324 1101 3 0.25662929417842406 1101 4 0.21555457084642882 1101 5 0.08685127415004956 1101 6 0.18760308903535616 1101 7 0.21999197157536088 1101 8 0.19317844030147313 1101 9 0.24963238255476483 1101 10-11 0.30086720392917954 1101 12-13 0.33493316716299404 1101 14-15 0.1472384516778007 1101 16-17 0.3846677872922939 1101 18-19 0.33188354505320916 1101 20-21 0.5090435074823034 1101 22-23 0.41827147337052395 1101 24-25 0.4336415146875936 1101 26-27 0.39965592914067827 1101 28-29 0.33838202808532714 1101 30-31 0.3173420326016796 1101 32-33 0.2539521306945218 1101 34-35 0.2387825794070224 1101 36-37 0.14259310323812002 1101 38-39 0.19073777774462997 1101 40-41 0.13536168146125505 1101 42-43 0.04383295019890454 1101 44-45 0.05921900961406834 1101 46-47 -0.03713011920100229 1101 48-49 -0.10075666227263014 1101 50-51 -0.14818598782056114 1101 52-53 -0.0894622076663012 1101 54-55 -0.13263460996582666 1101 56-57 -0.1071346564021951 1101 58-59 -0.19612537201070523 1101 60-61 -0.1565485516902143 1101 62-63 -0.1959501335581635 1101 64-65 -0.31618632178302875 1101 66-67 -0.2656890922795867 1101 68-69 -0.25309579148214567 1101 70-71 -0.42546772006706846 1101 72-73 -0.5042304252820742 1101 74-75 -0.40814389926364925 1101 76-77 -0.4012146109329855 1101 78-79 -0.5116628615796408 1101 80-81 -0.7565708134167011 1101 82-83 -0.7918051789582563 1101 84-85 -0.8172273087692652 1101 86-87 -0.7869586623277733 1101 88-89 -0.8493685164589344 1101 90-91 -0.6640175579417615 1101 92-93 -0.8697596378837211 1101 94-95 -1.0129067496348405 1101 96-97 -1.0160959320376115 1101 98-99 -1.1378436405842187 1101 100-101 -0.9983237153363937 1102 1 0.11905131423129589 1102 2 0.011808433510790195 1102 3 0.04611199373306363 1102 4 0.11271541360079596 1102 5 0.12102558305457478 1102 6 0.17022358604434373 1102 7 0.21093493149314213 1102 8 -0.03241251550531388 1102 9 0.2588047150184636 1102 10-11 0.15019900222680604 1102 12-13 0.2621243812247158 1102 14-15 0.30736145217850463 1102 16-17 0.2921851799715611 1102 18-19 0.2996428540894911 1102 20-21 0.40193606157090045 1102 22-23 0.38419269243922827 1102 24-25 0.33684614878252006 1102 26-27 0.35701586536751506 1102 28-29 0.3081194249810011 1102 30-31 0.287268838409787 1102 32-33 0.30138423995216357 1102 34-35 0.2623606949365822 1102 36-37 0.1508035678334707 1102 38-39 0.21628739299747934 1102 40-41 0.11613173574750135 1102 42-43 0.010305925664681581 1102 44-45 0.2848486707203435 1102 46-47 0.27767408139455796 1102 48-49 0.2754028496782439 1102 50-51 0.19322192980138908 1102 52-53 0.13565357882539075 1102 54-55 0.11799702758128205 1102 56-57 0.058815550465283195 1102 58-59 0.055949890855920614 1102 60-61 -0.07220311595089868 1102 62-63 -0.07652122613465195 1102 64-65 -0.009836346817948538 1102 66-67 -0.08919704415983887 1102 68-69 -0.1452175336488537 1102 70-71 -0.21775778911073473 1102 72-73 -0.24165037942864842 1102 74-75 -0.27941583775681167 1102 76-77 -0.36773287983434955 1102 78-79 -0.5297464716146862 1102 80-81 -0.4696558399010087 1102 82-83 -0.5220004183711247 1102 84-85 -0.7031545559824863 1102 86-87 -0.671368991207526 1102 88-89 -0.6515902006500447 1102 90-91 -0.6322281542998383 1102 92-93 -0.6419847149649129 1102 94-95 -0.7342681220753491 1102 96-97 -1.1118166082439025 1102 98-99 -1.2125547508169099 1102 100-101 -0.848484795263662 1103 1 0.07206920699318431 1103 2 0.21590730394715152 1103 3 0.19716111438314243 1103 4 0.2585563509977362 1103 5 0.20945063740362002 1103 6 0.19268679038074055 1103 7 0.20947795288377336 1103 8 0.21170313990093348 1103 9 0.22394428560498625 1103 10-11 0.3504675257257688 1103 12-13 0.3326758839477648 1103 14-15 0.23105747533328014 1103 16-17 0.332660409934455 1103 18-19 0.30793708652730345 1103 20-21 0.2887326656761289 1103 22-23 0.32521577517464095 1103 24-25 0.38774484904794093 1103 26-27 0.3076957086757801 1103 28-29 0.21542749041116593 1103 30-31 0.35525797180267205 1103 32-33 0.14858728525957332 1103 34-35 0.07952108544926517 1103 36-37 0.12243160585698831 1103 38-39 0.2177716862037684 1103 40-41 0.1584052267010705 1103 42-43 0.050157622411418856 1103 44-45 0.06338482043875615 1103 46-47 -0.0015955603019648379 1103 48-49 -0.18819589749224264 1103 50-51 -0.2632132891796388 1103 52-53 -0.1638733883858876 1103 54-55 -0.013531361189677682 1103 56-57 0.030655901949110387 1103 58-59 -0.04054827776538872 1103 60-61 -0.10381370865163575 1103 62-63 -0.12886709976952204 1103 64-65 -0.2803907310808995 1103 66-67 -0.26848555594053636 1103 68-69 -0.3545751143399727 1103 70-71 -0.35491638666527336 1103 72-73 -0.3296129866459445 1103 74-75 -0.4462352860699923 1103 76-77 -0.4008230632283549 1103 78-79 -0.37038260756574104 1103 80-81 -0.3208873380136019 1103 82-83 -0.3971079758498419 1103 84-85 -0.4870263570729847 1103 86-87 -0.2761386801292858 1103 88-89 -0.36489348992783377 1103 90-91 -0.45870962599831344 1103 92-93 -0.4914774839006313 1103 94-95 -0.455946186833156 1103 96-97 -0.46452289448326667 1103 98-99 -0.7096552012976503 1103 100-101 -0.5038674317445953 1104 1 -0.008506665092188115 1104 2 0.07304158066915889 1104 3 0.07069772360674875 1104 4 0.16363573133118337 1104 5 0.24998912173519727 1104 6 0.1413541366167408 1104 7 0.06937684120444487 1104 8 0.14125960078622768 1104 9 0.23720618407458005 1104 10-11 0.17321176168504593 1104 12-13 0.13608986179655602 1104 14-15 0.2646552572372869 1104 16-17 0.21053327784287745 1104 18-19 0.2891940152171415 1104 20-21 0.17232495611079202 1104 22-23 0.03696597509844679 1104 24-25 0.25475404611503194 1104 26-27 0.2911006027190268 1104 28-29 0.1772251686636892 1104 30-31 0.25438768831700287 1104 32-33 0.20643019033838783 1104 34-35 0.09467010447836088 1104 36-37 0.12924703414011418 1104 38-39 0.20919698563185563 1104 40-41 0.3069543347377177 1104 42-43 0.18945617872701348 1104 44-45 0.28222731022519554 1104 46-47 0.44250442791425826 1104 48-49 0.4000125449554446 1104 50-51 0.31725618568716385 1104 52-53 0.3473804055901937 1104 54-55 0.4605071454509968 1104 56-57 0.5268733664233096 1104 58-59 0.3693984893036415 1104 60-61 0.40512279966623055 1104 62-63 0.4381702982807738 1104 64-65 0.3358954225223698 1104 66-67 0.3019817223385317 1104 68-69 0.33889421294359323 1104 70-71 0.2870112105995233 1104 72-73 0.35049004704764997 1104 74-75 0.28105577210182986 1104 76-77 0.2833345053320677 1104 78-79 0.18828687805519095 1104 80-81 0.28782545013122984 1104 82-83 0.07443162622455901 1104 84-85 0.16059692355368327 1104 86-87 0.23243630645665903 1104 88-89 0.1625621407159379 1104 90-91 0.17783061102722897 1104 92-93 0.1298731201037917 1104 94-95 0.2105706084826231 1104 96-97 0.16532633036151267 1104 98-99 -0.08887862744825625 1104 100-101 0.4048603435353293 1105 1 -0.019999991154442398 1105 2 -0.018616978532666195 1105 3 0.048266481451186394 1105 4 0.02508083698639041 1105 5 0.07351940722759309 1105 6 0.1190740805558761 1105 7 0.05612339071338113 1105 8 0.07988175968122135 1105 9 0.14432310591862318 1105 10-11 0.01459942369730527 1105 12-13 0.23701745479721836 1105 14-15 0.16706551723846275 1105 16-17 0.1619858314025393 1105 18-19 0.0847509830217561 1105 20-21 0.15235795888589365 1105 22-23 0.17589529816314808 1105 24-25 0.2748587906706561 1105 26-27 0.04134258888346665 1105 28-29 0.11542842713956958 1105 30-31 0.1692251800636555 1105 32-33 0.05217616728524632 1105 34-35 0.18470310371694154 1105 36-37 0.32812403090172637 1105 38-39 0.2793731501449557 1105 40-41 0.16239523052328053 1105 42-43 0.01780302332291228 1105 44-45 -0.14730918326885956 1105 46-47 0.1470193398143138 1105 48-49 0.34834385716941085 1105 50-51 0.32074301446068887 1105 52-53 0.3247314024105137 1105 54-55 0.28874630310927074 1105 56-57 0.2213580092757752 1105 58-59 0.17588842207799615 1105 60-61 0.2850386906863349 1105 62-63 0.222010697397927 1105 64-65 0.21742944682035414 1105 66-67 0.11144542370656296 1105 68-69 0.04439881907995158 1105 70-71 0.03218607544268792 1105 72-73 0.08164191533644782 1105 74-75 0.018636939583842604 1105 76-77 0.06338243220821838 1105 78-79 0.1825582512599233 1105 80-81 0.08590377761339596 1105 82-83 -0.002458880738664959 1105 84-85 0.04745773792155816 1105 86-87 0.16393972220144448 1105 88-89 0.18913535138872106 1105 90-91 -0.024890314295326732 1105 92-93 0.1084550900214758 1105 94-95 0.07452062901210255 1105 96-97 -0.1393727102875637 1105 98-99 5.576729873375541E-4 1105 100-101 0.06267793777731256 1106 1 0.24299726948338574 1106 2 0.26155513309613454 1106 3 0.25181647291957177 1106 4 0.21755486179783645 1106 5 0.23923409160415332 1106 6 0.29889946407918444 1106 7 0.23434314992857708 1106 8 0.2285799532488113 1106 9 0.32366702069327147 1106 10-11 0.29471824426698134 1106 12-13 0.24931558549144484 1106 14-15 0.29287259519891506 1106 16-17 0.40747697911880465 1106 18-19 0.2813072374504202 1106 20-21 0.39579925004252203 1106 22-23 0.3641735346493675 1106 24-25 0.31480287708622967 1106 26-27 0.305090379279811 1106 28-29 0.3639788514927389 1106 30-31 0.21449341312882808 1106 32-33 0.30013870888210903 1106 34-35 0.16917590468324306 1106 36-37 0.22615705286622045 1106 38-39 0.20223324934702447 1106 40-41 0.19425714784144787 1106 42-43 0.3205654166462324 1106 44-45 0.3849308147810646 1106 46-47 0.27769422820990286 1106 48-49 0.39204723274612974 1106 50-51 0.3246237635278888 1106 52-53 0.11905800599647876 1106 54-55 0.04191926453713535 1106 56-57 0.19143818962483294 1106 58-59 0.24708510895435865 1106 60-61 0.32949352062986037 1106 62-63 0.15814640635813504 1106 64-65 -0.01011345710527678 1106 66-67 0.1861689868442724 1106 68-69 0.17263391838707776 1106 70-71 0.23280220077922564 1106 72-73 0.09094313842509649 1106 74-75 0.1432300068709509 1106 76-77 0.05059367073782184 1106 78-79 0.23237925550219174 1106 80-81 0.20970008472792045 1106 82-83 0.19806150561311142 1106 84-85 0.3753959539150955 1106 86-87 0.4543382535872702 1106 88-89 0.6680579810082925 1106 90-91 0.6445628975837074 1106 92-93 0.5636706792533168 1106 94-95 0.5862746697604315 1106 96-97 0.4951847763917705 1106 98-99 0.46359032090574814 1106 100-101 0.7953220737014526 1107 1 0.006566810410337354 1107 2 -0.09249645524673156 1107 3 0.017952351522623644 1107 4 0.04642100198210386 1107 5 0.012660838870665714 1107 6 0.040090118028167865 1107 7 -0.02235089696415571 1107 8 0.03441583274548066 1107 9 -1.1868076829557594E-4 1107 10-11 -0.017983055637074585 1107 12-13 -0.050326475800076764 1107 14-15 -0.024748209115195152 1107 16-17 0.06115795548070935 1107 18-19 0.16400196512535814 1107 20-21 0.07261203574967823 1107 22-23 -0.027775200338695072 1107 24-25 0.05653596334490629 1107 26-27 0.12540807222428896 1107 28-29 0.026752575899429587 1107 30-31 0.06732568642957659 1107 32-33 0.16058306956019663 1107 34-35 0.0030365328063695074 1107 36-37 -0.08910311073272936 1107 38-39 0.09065418396837543 1107 40-41 0.0250867516310862 1107 42-43 7.714710584423301E-4 1107 44-45 0.2008476326198263 1107 46-47 0.3269128315990244 1107 48-49 0.17129596765295219 1107 50-51 0.1904364736828299 1107 52-53 0.15458321971578215 1107 54-55 0.19205862991488942 1107 56-57 0.2106122822063341 1107 58-59 0.16798780340581487 1107 60-61 -0.04514336168011823 1107 62-63 -0.12726251383843135 1107 64-65 -0.06188718719869257 1107 66-67 -0.08795259695732938 1107 68-69 -0.034522174897951174 1107 70-71 0.10322538246926882 1107 72-73 0.15581047119679425 1107 74-75 0.05388302973076975 1107 76-77 0.17991491138620574 1107 78-79 0.22868020347922524 1107 80-81 0.06836880619782448 1107 82-83 0.15272303581327407 1107 84-85 0.13141539276534075 1107 86-87 0.22651432758618384 1107 88-89 0.2531239916977128 1107 90-91 0.2364110587812327 1107 92-93 0.250190216808857 1107 94-95 0.22023660282442847 1107 96-97 0.12317272378469113 1107 98-99 0.013678343282197858 1107 100-101 0.33887799898438686 1108 1 0.2250322624881811 1108 2 0.24215127485154042 1108 3 0.1393624231868813 1108 4 0.16799937475130378 1108 5 0.2438856382761898 1108 6 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2316 56-57 -1.2189585580892626 2316 58-59 -1.0947207901362859 2316 60-61 -0.9002430785633209 2316 62-63 -1.1333906775157416 2316 64-65 -1.5311171021634067 2316 66-67 -1.4869395385195574 2316 68-69 -1.5824313133092858 2316 70-71 -1.4820769048876485 2316 72-73 -1.304891576473807 2316 74-75 -1.468121428715488 2316 76-77 -1.3179883624072382 2316 78-79 -1.2043218559874482 2316 80-81 -1.0993161021966174 2316 82-83 -1.2950063434272252 2316 84-85 -1.4538488553321685 2316 86-87 -1.3170355562520548 2316 88-89 -1.4103885724524616 2316 90-91 -1.6357545847611696 2316 92-93 -1.6341111757622038 2316 94-95 -1.780491545458954 2316 96-97 -1.8093397821091664 2316 98-99 -2.1054891327165883 2316 100-101 -2.8061899799012053 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7456.0 3 1794.0 4 1854.0 5 2147.0 6 2578.0 7 3149.0 8 4039.0 9 4814.0 10 5954.0 11 7947.0 12 10579.0 13 15648.0 14 15374.0 15 13820.0 16 17564.0 17 23309.0 18 20161.0 19 14316.0 20 14629.0 21 16094.0 22 17257.0 23 19433.0 24 22489.0 25 25939.0 26 30052.0 27 35147.0 28 41731.0 29 50066.0 30 60320.0 31 73361.0 32 91931.0 33 118322.0 34 159445.0 35 228439.0 36 352768.0 37 544059.0 38 490734.0 39 77769.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.98332422503567 18.84912863952394 14.054558702978415 23.11298843246197 2 15.443284693484108 20.738521230411685 39.697343632467266 24.120850443636936 3 19.198286745190845 24.745570373479094 30.37171402371331 25.684428857616755 4 12.923793663589581 16.471431905105373 37.00510052550869 33.59967390579636 5 13.757380646246467 37.471218036772726 34.01999640864743 14.751404908333377 6 31.945623793245282 35.66244727016838 17.464264153258704 14.927664783327641 7 28.104906905348564 33.18760404612783 21.022743844351726 17.684745204171882 8 26.11223015696859 34.62751681157027 19.985144760933675 19.275108270527465 9 26.696858288516857 16.713433882848122 20.790666455557634 35.79904137307739 10-11 25.231082379106912 26.15626410886514 27.24781681882778 21.36483669320017 12-13 26.227601907733295 23.813845440876875 27.74144808721954 22.21710456417029 14-15 23.13209326128695 24.912481601864638 26.41654988392436 25.538875252924054 16-17 22.38277170893227 28.36009740285565 26.797905740124968 22.45922514808711 18-19 22.257770881859713 27.045153826660005 28.852390272765465 21.844685018714816 20-21 23.11149682443116 26.08633395483518 28.657150693109664 22.145018527624003 22-23 22.926324000728165 26.02669170162108 28.630584583991837 22.416399713658922 24-25 22.889545956856438 26.255060548495347 28.589965275008332 22.265428219639883 26-27 22.70516280819844 26.2453644006431 28.786394688769263 22.263078102389198 28-29 22.263464085384484 26.619870318304965 28.80631422965523 22.31035136665532 30-31 22.518957658844922 26.348678471551718 28.638808744305976 22.493555125297384 32-33 22.389822716590327 26.535818189085003 28.55446199458374 22.51989709974093 34-35 22.9271345687935 26.367213357633524 28.33575670431677 22.369895369256206 36-37 22.93943745184445 26.158626276407514 28.37309623800489 22.528840033743144 38-39 22.733482774691748 26.322435177320948 28.57593067971205 22.368151368275257 40-41 22.76845987096234 26.326136356070123 28.551032462968017 22.354371309999514 42-43 22.5240059202742 26.59337618897381 28.08120853362846 22.80140935712353 44-45 22.698376475500957 26.457143738093734 28.006478034237503 22.8380017521678 46-47 22.874534338796774 26.687004305730667 27.615405476128174 22.823055879344388 48-49 23.012760809112844 26.695786262395764 27.5015337373682 22.789919191123186 50-51 22.550829470957808 26.953338011770704 27.694388787281653 22.80144372998983 52-53 23.011364231175165 26.96588065891794 27.301068852527667 22.721686257379233 54-55 22.569794359126323 26.65288355708524 27.50898937756566 23.268332706222782 56-57 22.71597332195269 27.107161795739376 27.247428366916193 22.929436515391743 58-59 23.089673374320252 26.598320467368065 27.38275939091162 22.929246767400066 60-61 22.910886020877257 26.478837512131747 27.883268225007075 22.727008241983917 62-63 22.71203748300859 26.55387078744804 27.870270973865914 22.86382075567746 64-65 22.504115222812164 26.753774040525357 27.690561895673323 23.05154884098916 66-67 22.554007972326655 26.820670628142956 27.315204670457554 23.31011672907283 68-69 22.362023596170282 26.68118477015658 27.769966374686256 23.186825258986886 70-71 22.56800656300625 26.644240396567636 27.527253363226567 23.26049967719955 72-73 22.650662960812497 26.421153827849114 27.563673745228872 23.36450946610952 74-75 22.363256915322648 26.95461958420211 27.31682606835874 23.365297432116506 76-77 22.617523575900638 26.507142718469883 27.40362269270608 23.471711012923397 78-79 22.6361517883546 26.686157744525556 27.372316250223115 23.305374216896723 80-81 22.655878950252696 26.852527705047795 27.298472416732633 23.193120927966877 82-83 22.6755969701318 26.587622544588395 27.462453027032602 23.2743274582472 84-85 22.63681161202183 26.526067724486268 27.227666710885757 23.609453952606145 86-87 22.640267757213618 26.317971016313102 27.529269055311612 23.51249217116167 88-89 22.70868061252341 26.261025539319267 27.32118270143272 23.70911114672461 90-91 22.672251513012007 26.25587182671037 27.44336234450855 23.628514315769074 92-93 22.83617839963022 26.291245338708464 27.304697881296885 23.567878380364434 94-95 22.77581324512767 25.995402517668897 27.331908716655168 23.89687552054827 96-97 22.593520738411492 25.832732724843645 27.498731202667575 24.07501533407729 98-99 22.65219012793316 25.92471740422908 27.203877687817652 24.219214780020106 100-101 22.977922388139245 25.375825685934338 27.63472919975676 24.011522726169666 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2904.0 1 1731.5 2 558.5 3 551.5 4 519.0 5 543.0 6 867.5 7 1581.5 8 2460.5 9 3093.0 10 3146.5 11 3158.0 12 3462.0 13 3745.0 14 4044.0 15 4285.5 16 4579.0 17 4767.0 18 4844.0 19 5011.0 20 5359.5 21 5985.0 22 6904.0 23 7874.5 24 9081.5 25 11074.0 26 13737.0 27 16623.0 28 19099.5 29 22160.5 30 25950.0 31 30637.0 32 35460.0 33 39562.5 34 44866.5 35 49381.0 36 53727.5 37 59107.0 38 62922.5 39 64364.0 40 65867.0 41 70974.5 42 75691.0 43 80557.5 44 86873.0 45 90728.5 46 93493.5 47 100677.0 48 117905.0 49 119767.5 50 106951.0 51 101562.0 52 97357.0 53 97431.5 54 97159.5 55 91893.0 56 84541.5 57 75882.0 58 68503.0 59 61210.0 60 52134.5 61 41555.0 62 32937.0 63 26399.0 64 20221.0 65 15061.5 66 10233.5 67 7485.5 68 5202.5 69 2773.5 70 1813.5 71 1197.0 72 703.5 73 406.0 74 299.0 75 227.5 76 150.5 77 82.0 78 60.0 79 49.0 80 31.0 81 25.0 82 22.5 83 23.5 84 17.0 85 9.5 86 13.0 87 13.0 88 9.5 89 7.0 90 7.0 91 8.0 92 5.5 93 3.0 94 1.5 95 2.0 96 2.5 97 2.5 98 2.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28529812121026343 2 0.08616843374396736 3 0.08999057331715167 4 0.10486285898310653 5 0.10690637915094765 6 0.08147590595114701 7 0.09248820907784638 8 0.08904449916537338 9 0.0935856550939092 10-11 0.08760646645467036 12-13 0.09091772598589441 14-15 0.09935670741975683 16-17 0.08817411094573735 18-19 0.09884582737779656 20-21 0.10054876085099748 22-23 0.11313154706964884 24-25 0.12614952739811822 26-27 0.12912020023470208 28-29 0.12073798324994636 30-31 0.11347213376428902 32-33 0.10806058961611717 34-35 0.3839736552407399 36-37 0.5901610558793021 38-39 0.573642601189253 40-41 0.5372376678288241 42-43 0.5645413678491459 44-45 0.5853360777052328 46-47 0.5747022042392447 48-49 0.5958564222730075 50-51 0.5885905727873502 52-53 0.5909746796498315 54-55 0.581665309996333 56-57 0.5542291595947624 58-59 0.5489500658278395 60-61 0.4932830627372044 62-63 0.5702367342428512 64-65 0.6043710896390122 66-67 0.6164429958157033 68-69 0.6188271026781845 70-71 0.6154590786978539 72-73 0.6063956883238177 74-75 0.6039169740461586 76-77 0.5887608661346702 78-79 0.5665848880169869 80-81 0.5607949142081038 82-83 0.5748346546204937 84-85 0.5838602020284587 86-87 0.5749671050017426 88-89 0.5877012630846785 90-91 0.6004732641336855 92-93 0.6085527373898723 94-95 0.6078147995514852 96-97 0.605336085273826 98-99 0.6088365596354057 100-101 0.5871903830427183 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2642499.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.81165209708416 #Duplication Level Percentage of deduplicated Percentage of total 1 68.16380282141922 28.50041209183425 2 14.773300062505054 12.35392165078586 3 6.131325998505685 7.690826086299804 4 3.193670290935021 5.341305244694746 5 1.942016941747599 4.059946836749698 6 1.2324800692174935 3.091921672242723 7 0.8581083224276113 2.5115248647269066 8 0.631000708353364 2.110654567254762 9 0.46566169983970607 1.7523076489771163 >10 2.35355944422708 17.3590420985499 >50 0.15691041898898203 4.526366827243534 >100 0.09008483357852783 6.76302179229304 >500 0.005809178070821384 1.6069159231236034 >1k 0.00199690496184267 1.473553400889602 >5k 2.7230522206945503E-4 0.8582792943343125 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9050 0.34247884294374376 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7289 0.2758373796924805 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6276 0.23750245506242387 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4488 0.16983923172724003 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3292 0.12457904430616626 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.784296607113191E-5 0.0 5 0.0 0.0 0.0 3.784296607113191E-5 0.0 6 0.0 0.0 0.0 3.784296607113191E-5 0.0 7 0.0 0.0 0.0 3.784296607113191E-5 0.0 8 0.0 0.0 0.0 3.784296607113191E-5 0.0 9 0.0 0.0 0.0 3.784296607113191E-5 0.0 10-11 0.0 0.0 0.0 3.784296607113191E-5 0.0 12-13 0.0 0.0 0.0 1.1352889821339573E-4 0.0 14-15 0.0 0.0 0.0 1.1352889821339573E-4 0.0 16-17 0.0 0.0 0.0 2.459792794623574E-4 0.0 18-19 0.0 0.0 0.0 4.919585589247148E-4 0.0 20-21 0.0 0.0 0.0 6.054874571381106E-4 0.0 22-23 0.0 0.0 0.0 7.379378383870723E-4 0.0 24-25 0.0 0.0 0.0 0.0012109749142762212 0.0 26-27 0.0 0.0 0.0 0.002024598684805557 0.0 28-29 0.0 0.0 0.0 0.0061684034695945016 0.0 30-31 0.0 0.0 0.0 0.017748351087360864 0.0 32-33 0.0 0.0 0.0 0.04034060183182661 0.0 34-35 0.0 0.0 0.0 0.07538318841369476 0.0 36-37 0.0 0.0 0.0 0.13019872476772934 0.0 38-39 0.0 0.0 0.0 0.2107474780501336 0.0 40-41 0.0 0.0 0.0 0.3016084395869213 0.0 42-43 0.0 0.0 0.0 0.39167469883621525 0.0 44-45 0.0 0.0 0.0 0.5039926221353348 0.0 46-47 0.0 0.0 0.0 0.6371620197396479 0.0 48-49 0.0 0.0 0.0 0.7799624522090642 0.0 50-51 0.0 0.0 0.0 0.939262417885494 0.0 52-53 0.0 0.0 0.0 1.0955917107253401 0.0 54-55 0.0 0.0 0.0 1.272450812658775 0.0 56-57 0.0 0.0 0.0 1.486623079138346 0.0 58-59 0.0 0.0 0.0 1.7250148439034414 0.0 60-61 0.0 0.0 0.0 1.9829525006442767 0.0 62-63 0.0 0.0 0.0 2.246226015601141 0.0 64-65 0.0 0.0 0.0 2.5198306602954252 0.0 66-67 0.0 0.0 0.0 2.8338137497876064 0.0 68-69 0.0 0.0 0.0 3.1912405643294472 0.0 70-71 0.0 0.0 0.0 3.5942870744700377 0.0 72-73 0.0 0.0 0.0 4.000379943379354 0.0 74-75 0.0 0.0 0.0 4.41897991257518 0.0 76-77 0.0 0.0 0.0 4.8730765839457275 0.0 78-79 0.0 0.0 0.0 5.38736249285241 0.0 80-81 0.0 0.0 0.0 5.948176328543549 0.0 82-83 0.0 0.0 0.0 6.524297644010462 3.784296607113191E-5 84-85 0.0 0.0 0.0 7.13008784487714 3.784296607113191E-5 86-87 0.0 0.0 0.0 7.750561116579419 3.784296607113191E-5 88-89 0.0 3.784296607113191E-5 0.0 8.413550960662615 3.784296607113191E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCGT 495 0.0 28.684015 3 GTACATG 10860 0.0 25.27535 1 TACATGG 11375 0.0 23.75793 2 ACATGGG 11225 0.0 22.979155 3 GTATCAA 20180 0.0 21.706972 1 GAGTACT 8270 0.0 21.66129 12-13 CATGGGG 6150 0.0 20.162764 4 AGTACTT 8740 0.0 19.467552 12-13 CCGTCTA 195 1.0662272E-5 19.416872 9 GGTATCA 15595 0.0 19.333931 1 GTACTTT 9295 0.0 19.145851 14-15 TATCAAC 23075 0.0 18.808386 2 ATCAACG 23035 0.0 18.738314 3 CAACGCA 23110 0.0 18.718462 5 TCAACGC 23210 0.0 18.617422 4 AACGCAG 23380 0.0 18.500193 6 CTTTGCG 795 0.0 18.455189 2 CATGGGA 5615 0.0 18.375137 4 TATAGGG 345 1.1386874E-9 17.833975 2 AGAGTAC 16865 0.0 17.553547 10-11 >>END_MODULE