FastQCFastQC Report
Fri 27 May 2016
SRR939052_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939052_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2642499
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84950.3214759967742656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73570.2784107013853175No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT63580.2406055782802567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45030.170406876218307No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33420.12647119260972284No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG109250.024.6848431
TACATGG115500.023.0075822
ACATGGG111800.022.7503413
TACGGTA1150.00374589920.63185
GAGTACT81150.020.34997612-13
CATGGGG58700.019.8058244
AGTACTT84350.019.52169612-13
TTTGCGT5050.018.7933223
GTACTTT88150.018.62631414-15
GTATCAA211700.018.6131551
GGTATCA170500.018.2763211
CATGGGA60900.017.2202684
AGTACAT84150.017.0865142
CCCTATA3351.4773832E-816.998142
CTCGCGC6150.016.97655160-61
AGAGTAC168650.016.62931310-11
ATGGGGA33350.016.0785755
CAACGCA241150.016.0768035
CCTCGCG6200.016.0737558-59
TACTTTT102300.016.04994814-15
Produced by FastQC (version 0.11.5)