##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939052_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2642499 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17287764347309 33.0 31.0 34.0 30.0 34.0 2 32.4029549301627 34.0 31.0 34.0 31.0 34.0 3 32.49946546810425 34.0 31.0 34.0 30.0 34.0 4 36.07015064149504 37.0 35.0 37.0 35.0 37.0 5 35.98131352178373 37.0 35.0 37.0 35.0 37.0 6 36.02081855092471 37.0 35.0 37.0 35.0 37.0 7 35.960784091119805 37.0 35.0 37.0 35.0 37.0 8 35.93084046578636 37.0 35.0 37.0 35.0 37.0 9 37.6114594556138 39.0 37.0 39.0 35.0 39.0 10-11 37.534990930933176 39.0 37.0 39.0 35.0 39.0 12-13 37.46140887848964 39.0 37.0 39.0 34.0 39.0 14-15 38.732556379396925 40.0 38.0 41.0 34.0 41.0 16-17 38.719860442709724 40.0 38.0 41.0 34.0 41.0 18-19 38.81039084593788 40.0 38.0 41.0 34.0 41.0 20-21 38.81060598319999 40.0 38.0 41.0 34.0 41.0 22-23 38.74934711422786 40.0 38.0 41.0 34.0 41.0 24-25 38.682690703004994 40.0 38.0 41.0 34.0 41.0 26-27 38.53350427001108 40.0 38.0 41.0 34.0 41.0 28-29 38.35282378536378 40.0 38.0 41.0 34.0 41.0 30-31 38.19162731944269 40.0 38.0 41.0 33.5 41.0 32-33 38.05152641495796 40.0 37.0 41.0 33.0 41.0 34-35 38.02905715385323 40.0 37.0 41.0 33.0 41.0 36-37 37.92565162749352 40.0 37.0 41.0 33.0 41.0 38-39 37.80386142057196 40.0 37.0 41.0 33.0 41.0 40-41 37.644582457741706 40.0 37.0 41.0 33.0 41.0 42-43 37.47671768276923 40.0 37.0 41.0 32.0 41.0 44-45 37.238195738200844 40.0 37.0 41.0 31.5 41.0 46-47 36.93031444855798 40.0 36.0 41.0 31.0 41.0 48-49 36.65516997357426 39.5 36.0 41.0 30.5 41.0 50-51 36.38865388406958 39.0 35.5 41.0 30.0 41.0 52-53 36.14835634753315 39.0 35.0 41.0 29.5 41.0 54-55 35.886500619300136 39.0 35.0 40.0 29.0 41.0 56-57 35.607326814504 38.0 35.0 40.0 28.0 41.0 58-59 35.29195072543074 38.0 34.5 40.0 28.0 41.0 60-61 34.88438160241499 38.0 34.0 40.0 27.0 41.0 62-63 34.566165587952916 37.0 34.0 40.0 26.0 41.0 64-65 34.293431709907935 37.0 34.0 39.5 26.0 41.0 66-67 33.85135623513954 36.0 33.0 39.0 26.0 40.5 68-69 33.43155058904469 36.0 33.0 39.0 26.0 40.0 70-71 32.97689573392459 35.0 32.5 38.0 25.0 40.0 72-73 32.475638779806545 35.0 32.0 37.0 23.5 39.5 74-75 32.01374324077322 35.0 31.5 37.0 22.5 39.0 76-77 30.02612016125645 33.0 29.5 35.0 20.0 37.0 78-79 31.024830283757915 34.0 31.0 36.0 20.0 37.5 80-81 31.037957630258326 34.0 31.0 35.5 20.0 37.0 82-83 30.73157378678289 34.0 31.0 35.0 19.5 37.0 84-85 30.354747910973664 34.0 30.5 35.0 18.0 36.0 86-87 29.80056567665683 34.0 30.0 35.0 11.0 36.0 88-89 29.252472754010505 34.0 29.0 35.0 6.0 35.5 90-91 28.803913643865144 34.0 29.0 35.0 2.0 35.0 92-93 28.46282344856138 33.5 29.0 35.0 2.0 35.0 94-95 28.085649985108795 33.0 28.5 35.0 2.0 35.0 96-97 27.487766882787845 33.0 27.0 35.0 2.0 35.0 98-99 26.63470109165604 33.0 26.0 34.5 2.0 35.0 100-101 22.380715186647187 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1272007488518625 1101 2 0.14406224651815336 1101 3 0.20245127030576526 1101 4 0.1404556088569322 1101 5 0.09534468706351618 1101 6 0.0910072539313731 1101 7 0.04964172070184247 1101 8 0.11541087173667819 1101 9 0.2221086176898197 1101 10-11 0.26687645848863184 1101 12-13 0.2455752818614556 1101 14-15 0.2631438551641878 1101 16-17 0.15926848029499752 1101 18-19 0.2649748217885701 1101 20-21 0.13005275381190273 1101 22-23 0.1245766253659113 1101 24-25 0.22283786331070132 1101 26-27 0.2464746453760469 1101 28-29 0.22663290990660556 1101 30-31 0.17614541102971515 1101 32-33 0.28561603683245806 1101 34-35 0.36418591994355864 1101 36-37 0.25893613582577757 1101 38-39 0.2578924004075631 1101 40-41 0.3015431480454609 1101 42-43 0.27001718868417157 1101 44-45 0.2943470361529208 1101 46-47 0.2607800084266785 1101 48-49 -0.10095288696535931 1101 50-51 0.01829157124737435 1101 52-53 0.1191628479453044 1101 54-55 0.16267970874464766 1101 56-57 -0.0022819781023883934 1101 58-59 -0.013501240220612942 1101 60-61 0.2623924704115055 1101 62-63 0.16392202541201328 1101 64-65 0.13386114137521332 1101 66-67 0.21235152044889105 1101 68-69 0.16359001640212512 1101 70-71 0.1094128199075044 1101 72-73 0.03273787123712424 1101 74-75 0.1970691753232643 1101 76-77 -0.03959773488249141 1101 78-79 -0.0010181390725136907 1101 80-81 0.056458932217363866 1101 82-83 0.03618191169938001 1101 84-85 -0.034159390904385134 1101 86-87 0.12654557610315464 1101 88-89 0.25627336772834397 1101 90-91 0.35455858041537525 1101 92-93 0.4815773306384763 1101 94-95 0.38269759092446876 1101 96-97 0.1287403270085541 1101 98-99 -0.03924835714588326 1101 100-101 0.3739959270976989 1102 1 0.1144938813788059 1102 2 0.024456282937364904 1102 3 0.07375862003038236 1102 4 0.029756805262444175 1102 5 0.06523855219118957 1102 6 0.07233318219974905 1102 7 0.08637716750922664 1102 8 0.1100053060793087 1102 9 0.17398063655824103 1102 10-11 0.1751737217769005 1102 12-13 0.143870107539378 1102 14-15 0.16814540337616535 1102 16-17 0.17702680111086977 1102 18-19 0.1644365710704605 1102 20-21 0.1991565367196486 1102 22-23 0.16062525898649227 1102 24-25 0.17441506308934152 1102 26-27 0.19236546019672573 1102 28-29 0.22497022906227215 1102 30-31 0.15576254150116142 1102 32-33 0.08362460799749982 1102 34-35 0.16829345808886842 1102 36-37 0.17339660042113536 1102 38-39 0.11523207811595171 1102 40-41 0.20327615724250592 1102 42-43 0.13934892110043506 1102 44-45 0.1959631059344531 1102 46-47 0.17489624291162187 1102 48-49 -0.006851245201843881 1102 50-51 0.07741350447003015 1102 52-53 0.09229807602658013 1102 54-55 0.02226347030683229 1102 56-57 -0.08034727311913059 1102 58-59 0.008075728973260254 1102 60-61 -0.08804448771430629 1102 62-63 0.12374180694941828 1102 64-65 -0.1235011006992579 1102 66-67 -0.03408923903143801 1102 68-69 0.030866250360013225 1102 70-71 0.003460768361925659 1102 72-73 -0.08437118876762639 1102 74-75 -0.030089756871639395 1102 76-77 -0.2511296082848702 1102 78-79 -0.19222231858604744 1102 80-81 -7.623237448974862E-4 1102 82-83 -0.035615595349931084 1102 84-85 -0.04567627629716853 1102 86-87 0.00785371640410304 1102 88-89 0.005977725121550037 1102 90-91 0.08470605584162882 1102 92-93 -0.05399860421803382 1102 94-95 -0.06023515079424513 1102 96-97 -0.06583697818026124 1102 98-99 0.09314023747292666 1102 100-101 0.39732945807511655 1103 1 0.03557430675812867 1103 2 0.061627547261025484 1103 3 0.08095166399306919 1103 4 0.0814966550381655 1103 5 0.07388017027189164 1103 6 0.021264691198155106 1103 7 0.08655846439428672 1103 8 0.11062243587347353 1103 9 0.033776311366857215 1103 10-11 0.12158658481090612 1103 12-13 0.06108356641178858 1103 14-15 0.16042998025405097 1103 16-17 0.054335835058616055 1103 18-19 0.08284736980129992 1103 20-21 0.08491778753208479 1103 22-23 0.21015012507492514 1103 24-25 0.19575046472797908 1103 26-27 0.15937132813255062 1103 28-29 0.1732733022245725 1103 30-31 0.12140147894933051 1103 32-33 0.13875997233620296 1103 34-35 0.1864724729544136 1103 36-37 0.16144931579223254 1103 38-39 0.17300785349030434 1103 40-41 0.11058982566691355 1103 42-43 0.22659604596396576 1103 44-45 0.27534092544119915 1103 46-47 0.2663558189090267 1103 48-49 0.12229777203296521 1103 50-51 -0.0014548427747342885 1103 52-53 0.08285803126666025 1103 54-55 0.16724134648385558 1103 56-57 0.12671908314165847 1103 58-59 0.08129395679421236 1103 60-61 0.04761250738387446 1103 62-63 0.1638749541032567 1103 64-65 0.13064531514537236 1103 66-67 0.12159375796215244 1103 68-69 0.18898284786972397 1103 70-71 0.005455834525285752 1103 72-73 -0.009795335431597607 1103 74-75 0.062415277818899995 1103 76-77 0.0782131925078744 1103 78-79 0.18976401491057615 1103 80-81 0.23666715857045162 1103 82-83 0.3088767013334177 1103 84-85 0.14693035268535581 1103 86-87 -0.03746370122388143 1103 88-89 0.27427600372163496 1103 90-91 0.3380023173637845 1103 92-93 0.30287110695561736 1103 94-95 0.21288570499109127 1103 96-97 0.1467408233969003 1103 98-99 0.17583542978369948 1103 100-101 0.5960676637722386 1104 1 -0.04663184528921249 1104 2 0.021412830809609318 1104 3 0.07154008637401432 1104 4 0.010430749397620787 1104 5 0.04442987779394514 1104 6 -5.70191759180716E-4 1104 7 -0.04413564589043517 1104 8 -0.013694705633682247 1104 9 -0.00941981531676106 1104 10-11 0.007964365868311063 1104 12-13 -0.051006189366539445 1104 14-15 -0.004706225346559734 1104 16-17 -0.027008556721121124 1104 18-19 -0.09202561136868326 1104 20-21 -0.06228848464115089 1104 22-23 -0.12230829134548316 1104 24-25 -0.02516820089073235 1104 26-27 -0.06892621340527683 1104 28-29 -0.09753879456714998 1104 30-31 -0.15338249465500553 1104 32-33 -0.021414175885418274 1104 34-35 0.01832339557847007 1104 36-37 -0.05282467191832296 1104 38-39 0.10599133446407905 1104 40-41 0.07117823660728817 1104 42-43 -0.15988784629798403 1104 44-45 -0.04644001527038455 1104 46-47 0.014787890005251825 1104 48-49 -0.2011852381617132 1104 50-51 -0.24136913009070327 1104 52-53 -0.0981902787908524 1104 54-55 -0.21888474222853915 1104 56-57 -0.2858893304758112 1104 58-59 -0.10017565192715239 1104 60-61 -0.1654756661648591 1104 62-63 -0.13201971493846543 1104 64-65 -0.1548175642379377 1104 66-67 -0.17060303570617918 1104 68-69 -0.1332356943053341 1104 70-71 -0.4037684611079655 1104 72-73 -0.30351515380124994 1104 74-75 -0.27947559524464083 1104 76-77 -0.4356670682911563 1104 78-79 -0.421441385491903 1104 80-81 -0.21929097126390218 1104 82-83 -0.18079161391810672 1104 84-85 -0.20513232328477216 1104 86-87 -0.2807552421873787 1104 88-89 -0.3665161178503453 1104 90-91 -0.19929534201181198 1104 92-93 -0.343434793056165 1104 94-95 -0.2341336284131792 1104 96-97 -0.11275484234651145 1104 98-99 -0.13811473284387787 1104 100-101 0.5568265390735547 1105 1 -0.018580901056900245 1105 2 0.032032990865289435 1105 3 -2.057766629093294E-6 1105 4 0.02946304522301091 1105 5 0.08270029472452478 1105 6 0.0435253988762625 1105 7 0.011354601987562774 1105 8 0.09145036051863542 1105 9 -0.07889999331673181 1105 10-11 -0.025873292448281404 1105 12-13 0.0029425792359702996 1105 14-15 -0.04623085665583915 1105 16-17 -0.0035907147814882023 1105 18-19 -0.09200722148113982 1105 20-21 -0.030242867817797503 1105 22-23 -0.09356569283315963 1105 24-25 0.046110030681091985 1105 26-27 -0.07717867543988177 1105 28-29 0.02231208112745975 1105 30-31 -0.08120837495593491 1105 32-33 -0.10088536759609923 1105 34-35 -0.04586252830649329 1105 36-37 -0.03665138471531293 1105 38-39 0.07256329320990318 1105 40-41 0.044930031831647455 1105 42-43 0.10582622419399002 1105 44-45 -0.0786420345507608 1105 46-47 -0.161752112774451 1105 48-49 -0.2541574860531597 1105 50-51 -0.2207206460716904 1105 52-53 -0.00554405884992093 1105 54-55 -0.005131741051940253 1105 56-57 -0.04687560843684224 1105 58-59 -0.07592451820827506 1105 60-61 -0.10370182358725089 1105 62-63 -0.1767272998122209 1105 64-65 -0.251516455646005 1105 66-67 -0.03688636163051484 1105 68-69 -0.03241658395858593 1105 70-71 0.02348803998251725 1105 72-73 -0.07421818859243956 1105 74-75 0.059655326962243294 1105 76-77 -0.24064814615783092 1105 78-79 0.011008225332101773 1105 80-81 -0.10405135987052105 1105 82-83 0.12275372652645444 1105 84-85 -0.11826627487198138 1105 86-87 -0.18393725857488974 1105 88-89 -0.1186094705779368 1105 90-91 0.0777650238550045 1105 92-93 0.18641234807667217 1105 94-95 0.016857745668612978 1105 96-97 -0.1643709244446292 1105 98-99 -0.13343114677336843 1105 100-101 0.3342632787445403 1106 1 0.056798713684045765 1106 2 -0.007811405966499763 1106 3 -0.06350163286710142 1106 4 0.03646770945151445 1106 5 0.04369409732738205 1106 6 -0.004093944937643812 1106 7 0.06967838803993232 1106 8 0.04760072872286969 1106 9 0.07165111524657419 1106 10-11 0.11453830953642807 1106 12-13 0.1427024164376718 1106 14-15 0.15396415163620958 1106 16-17 0.09950789973502339 1106 18-19 0.035995128705003765 1106 20-21 0.07593998775922017 1106 22-23 0.15181853654366506 1106 24-25 0.09458312415982562 1106 26-27 0.1627748251691017 1106 28-29 0.14177441300636673 1106 30-31 0.16309717277781033 1106 32-33 0.11161357120575843 1106 34-35 0.10097644611800405 1106 36-37 0.04748990215449567 1106 38-39 0.15036748303030123 1106 40-41 0.1951031437184909 1106 42-43 0.34780957620160535 1106 44-45 0.23614043453999756 1106 46-47 0.15267421401741643 1106 48-49 0.09448943378246355 1106 50-51 0.08528266738817081 1106 52-53 0.26056299565910024 1106 54-55 0.2093046723757226 1106 56-57 0.2757448491097705 1106 58-59 0.20354077241712076 1106 60-61 0.17911592855552527 1106 62-63 0.14678069762913992 1106 64-65 0.19521803804270377 1106 66-67 0.2972106701247412 1106 68-69 0.3906913943181607 1106 70-71 0.2862740696557182 1106 72-73 0.34364826939293636 1106 74-75 0.3945783701338854 1106 76-77 0.3006048493628626 1106 78-79 0.5309368119506672 1106 80-81 0.6348603593713342 1106 82-83 0.6741539617131806 1106 84-85 0.4983123718881508 1106 86-87 0.5426040424162046 1106 88-89 0.6608896102773762 1106 90-91 0.7472704340742773 1106 92-93 0.8015441509871692 1106 94-95 0.9344304303864313 1106 96-97 0.7225894090195517 1106 98-99 0.8191389700550893 1106 100-101 1.1797160131894735 1107 1 -0.08822609262205106 1107 2 -0.08400100787138598 1107 3 -0.07552661871121558 1107 4 -0.07902690968820991 1107 5 -0.11285038870236264 1107 6 -0.08596279768965331 1107 7 -0.1180888169429366 1107 8 -0.14242816796315338 1107 9 -0.06505563741420417 1107 10-11 -0.023957649233189215 1107 12-13 -0.038749761862270304 1107 14-15 -0.048721201066648234 1107 16-17 -0.05446082490319526 1107 18-19 -0.04321079328775568 1107 20-21 -0.030080466577594223 1107 22-23 -0.0720118463272641 1107 24-25 -0.0668970328262688 1107 26-27 -0.037998244451841856 1107 28-29 -0.029822914882878138 1107 30-31 -0.045001129572348475 1107 32-33 0.06983076672428723 1107 34-35 -0.14005961015458723 1107 36-37 0.008392694092670183 1107 38-39 -0.023532887175484518 1107 40-41 -0.04463544566504396 1107 42-43 -0.08748491896751887 1107 44-45 -0.13034176560508826 1107 46-47 -0.24351072477310964 1107 48-49 -0.13287248644578398 1107 50-51 -0.11092802414194125 1107 52-53 -0.0337825313662421 1107 54-55 -0.07230519067982044 1107 56-57 -0.09518047038261557 1107 58-59 -0.09949187669489135 1107 60-61 -0.07467463911770977 1107 62-63 -0.13292719968810474 1107 64-65 -0.20136204688447634 1107 66-67 -4.589134022126018E-5 1107 68-69 -0.11955600258354337 1107 70-71 -0.0906207673668078 1107 72-73 -0.05611361931460834 1107 74-75 -0.17823071757734255 1107 76-77 -0.508358647550466 1107 78-79 -0.18255388659848748 1107 80-81 0.08856400139363174 1107 82-83 0.03701574911578476 1107 84-85 -0.053536910406556615 1107 86-87 0.05999656534791953 1107 88-89 0.10626463759396287 1107 90-91 0.29634556951987534 1107 92-93 0.3293680769882563 1107 94-95 0.19887109631816102 1107 96-97 0.16160334718210478 1107 98-99 0.12383612352435946 1107 100-101 0.8865825709418225 1108 1 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2316 98-99 -3.035552940350076 2316 100-101 -3.8448319091728926 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 26.0 5 74.0 6 120.0 7 338.0 8 762.0 9 1378.0 10 2353.0 11 3760.0 12 5412.0 13 7231.0 14 8851.0 15 11381.0 16 14892.0 17 21172.0 18 20523.0 19 13909.0 20 13228.0 21 14628.0 22 15437.0 23 17087.0 24 19698.0 25 23606.0 26 28620.0 27 35075.0 28 42922.0 29 51902.0 30 63466.0 31 78122.0 32 97115.0 33 123915.0 34 167676.0 35 242693.0 36 386815.0 37 612222.0 38 462552.0 39 33528.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.71197247695964 19.526303669966705 14.568265362965036 23.193458490108622 2 15.681633181318139 21.168598360869765 38.948926754560745 24.200841703251356 3 19.155882367410545 24.836338632483873 30.50059810807875 25.507180892026827 4 13.221386271101712 16.961633665708103 36.596494454680965 33.22048560850922 5 13.773939132247964 37.622001730947744 34.02640622475149 14.577652912052793 6 32.04655895801663 35.88130023890265 17.21423546423291 14.85790533884781 7 28.079026709187023 33.21825287351102 20.883262396693432 17.819458020608522 8 26.12038831424345 34.93060924526367 19.721483338309685 19.227519102183198 9 26.33764478245782 16.735408414534877 20.66021595467018 36.266730848337126 10-11 25.226026575601352 26.2942767433403 27.163889182171875 21.31580749888647 12-13 26.08704866113478 23.808296616195506 27.74678438856552 22.357870334104195 14-15 22.917613542829958 24.999914852101885 26.327427348975064 25.75504425609309 16-17 22.333329612023135 28.41419015049925 26.828666021933905 22.42381421554371 18-19 22.219270470868675 27.154977163662124 28.757267268596888 21.868485096872316 20-21 22.990207375669776 26.175544437292125 28.58659549161608 22.247652695422023 22-23 22.825420179913028 26.123907710088062 28.534788471064697 22.515883638934206 24-25 22.79003700663652 26.29635810647421 28.601127190587395 22.312477696301872 26-27 22.649015193572446 26.360861442142458 28.71798626981505 22.272137094470047 28-29 22.263300761892435 26.718818058209294 28.74027577683095 22.277605403067323 30-31 22.458683995717692 26.359915367990677 28.627257758659514 22.55414287763212 32-33 22.35465368198815 26.606462291944105 28.501959697997993 22.536924328069755 34-35 22.85210096309043 26.404549447081116 28.342178092824373 22.401171497004086 36-37 22.885424521652084 26.19474150810043 28.373508282091624 22.546325688155857 38-39 22.645464917554538 26.40221063187215 28.593880605516624 22.358443845056687 40-41 22.666709556440892 26.411194987951443 28.513765252281754 22.408330203325914 42-43 22.511327414405315 26.62813724818665 28.073368873139394 22.787166464268644 44-45 22.652336490331653 26.50885207090739 27.949614927441512 22.889196511319444 46-47 22.837396889489753 26.73547796256754 27.54374894157694 22.88337620636577 48-49 22.902838952215127 26.739373371090664 27.482782331355022 22.875005345339186 50-51 22.456742854493832 26.966271299809936 27.702416193863154 22.874569651833077 52-53 22.917435351914985 27.000842762854404 27.25442847849706 22.827293406733553 54-55 22.48574171645855 26.669395144520397 27.46984956285698 23.37501357616408 56-57 22.621503357238733 27.13276334257837 27.2107198526849 23.03501344749799 58-59 23.055042519499104 26.653764711247163 27.294087951398932 22.9971048178548 60-61 22.8077734244764 26.61009393215889 27.751255563515375 22.830877079849333 62-63 22.624909224185135 26.70127784343532 27.727862905529953 22.945950026849584 64-65 22.402647655297713 26.815986745829406 27.618412035016988 23.162953563855897 66-67 22.477691677305444 26.90419952199763 27.21131916885639 23.406789631840535 68-69 22.22449073814775 26.729757058974013 27.630303218374447 23.415448984503787 70-71 22.370964000364808 26.721429222868203 27.424854276198403 23.48275250056859 72-73 22.51206149936102 26.529773521200955 27.352290388757005 23.605874590681015 74-75 22.197718901691164 27.055469084378082 27.061050921873576 23.685761092057177 76-77 22.310487657795516 26.55348692782163 27.317790238508028 23.81823517587483 78-79 22.26445083150583 26.733730811221967 27.28932315860579 23.71249519866641 80-81 22.242112909430222 26.92907867308156 27.172427912447255 23.656380505040968 82-83 22.18087878178951 26.662394195797233 27.405421913120875 23.751305109292378 84-85 22.131607239964897 26.534390363061632 27.117266647972244 24.21673574900123 86-87 22.087483094443154 26.356523484156387 27.421500135206557 24.1344932861939 88-89 22.110066757978146 26.3013738722345 27.207482245289338 24.381077124498013 90-91 21.989703643708708 26.30793836311629 27.32690399623626 24.375453996938738 92-93 22.06447381815471 26.36267790451387 27.18721937075473 24.38562890657669 94-95 21.959156843578747 26.045989042947603 27.250398202610484 24.744455910863163 96-97 21.643457132861137 25.910028989763234 27.401523074212925 25.044990803162705 98-99 21.577094258124603 26.018269070300498 27.126330038346282 25.27830663322862 100-101 21.67852478700984 25.44587629938849 27.745617490755887 25.129981422845777 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 779.0 1 524.5 2 329.5 3 407.0 4 377.0 5 413.5 6 686.5 7 1309.5 8 2072.5 9 2535.5 10 2517.0 11 2412.0 12 2593.5 13 2826.0 14 3054.0 15 3242.0 16 3423.5 17 3597.0 18 3813.5 19 4116.5 20 4532.0 21 5247.0 22 6391.5 23 7665.5 24 9056.5 25 11006.0 26 13803.5 27 16923.0 28 19503.0 29 22717.0 30 26515.5 31 31003.0 32 35644.5 33 39579.0 34 45007.0 35 49696.0 36 53930.5 37 59213.5 38 62889.0 39 64294.5 40 66234.5 41 71255.0 42 75857.5 43 81030.5 44 87587.0 45 91695.5 46 94323.5 47 101851.0 48 119185.0 49 120529.5 50 107652.5 51 102938.5 52 99423.0 53 98829.0 54 98032.0 55 92602.5 56 84888.0 57 76334.5 58 69523.0 59 62144.5 60 52627.5 61 41690.5 62 32763.5 63 26122.0 64 19844.5 65 14644.5 66 9700.0 67 7016.5 68 4705.5 69 2376.5 70 1519.5 71 959.0 72 540.5 73 304.5 74 195.0 75 134.5 76 78.0 77 34.0 78 18.5 79 12.0 80 8.0 81 5.5 82 5.5 83 5.0 84 2.5 85 3.0 86 4.5 87 3.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4091959921271493 2 0.0 3 0.0 4 0.0 5 6.054874571381105E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0014380327107030126 16-17 0.0022894994473034807 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00257332169283697 36-37 0.00762535766333308 38-39 0.01356670333650079 40-41 0.002516557243730272 42-43 6.054874571381105E-4 44-45 3.784296607113191E-4 46-47 2.4597927946235745E-4 48-49 3.784296607113191E-4 50-51 6.244089401736766E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.081363133912255E-4 60-61 0.0022705779642679144 62-63 0.0 64-65 3.784296607113191E-4 66-67 0.0016461690240942382 68-69 0.0017786194053431997 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12728481638025216 78-79 1.5137186428452762E-4 80-81 1.8921483035565953E-5 82-83 0.0 84-85 0.0 86-87 0.1492337367015087 88-89 0.43812693968852967 90-91 0.33854317447234605 92-93 0.0 94-95 0.0 96-97 5.487230080314127E-4 98-99 0.0 100-101 2.2705779642679145E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2642499.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.96105420284424 #Duplication Level Percentage of deduplicated Percentage of total 1 65.92336508967016 26.343671655821986 2 15.612134442387337 12.477547013486635 3 6.66255765275172 7.987284824735585 4 3.4660630138254036 5.540301278638025 5 2.058723706484744 4.113438481175863 6 1.355715980617387 3.2505503871068124 7 0.9239738503854114 2.584607838208348 8 0.6617239245432568 2.1154548492793515 9 0.5092739732794437 1.8316012365285925 >10 2.553277482113194 18.029700488320685 >50 0.16415758332271096 4.520616626490616 >100 0.10021212356782988 7.1497669172456675 >500 0.00644989289786583 1.7285181370014964 >1k 0.0020867300551900146 1.4851011803128955 >5k 2.84554098435002E-4 0.841839085647365 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8495 0.3214759967742656 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7357 0.2784107013853175 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6358 0.2406055782802567 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4503 0.170406876218307 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3342 0.12647119260972284 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.784296607113191E-5 0.0 8 0.0 0.0 0.0 3.784296607113191E-5 0.0 9 0.0 0.0 0.0 3.784296607113191E-5 0.0 10-11 0.0 0.0 0.0 3.784296607113191E-5 0.0 12-13 0.0 0.0 0.0 1.1352889821339573E-4 0.0 14-15 0.0 0.0 0.0 1.1352889821339573E-4 0.0 16-17 0.0 0.0 0.0 1.5137186428452765E-4 0.0 18-19 0.0 0.0 0.0 3.2166521160462125E-4 0.0 20-21 0.0 0.0 0.0 3.784296607113191E-4 0.0 22-23 0.0 0.0 0.0 5.865659741025445E-4 0.0 24-25 0.0 0.0 0.0 0.0011542104651695233 0.0 26-27 0.0 0.0 0.0 0.001986755718734425 0.0 28-29 0.0 0.0 0.0 0.006187324952630068 0.0 30-31 0.0 0.0 0.0 0.018088937782001054 0.0 32-33 0.0 0.0 0.0 0.04119206856842708 0.0 34-35 0.0 3.784296607113191E-5 0.0 0.07695367150564675 0.0 36-37 0.0 3.784296607113191E-5 0.0 0.1322233234525349 0.0 38-39 0.0 3.784296607113191E-5 0.0 0.21409658054742878 0.0 40-41 0.0 3.784296607113191E-5 0.0 0.3063198888627773 0.0 42-43 0.0 3.784296607113191E-5 0.0 0.39799447417009426 0.0 44-45 0.0 3.784296607113191E-5 0.0 0.5121856242897349 0.0 46-47 0.0 3.784296607113191E-5 0.0 0.6477202072734938 0.0 48-49 0.0 3.784296607113191E-5 0.0 0.7921668087670043 0.0 50-51 0.0 3.784296607113191E-5 0.0 0.9527534352898526 0.0 52-53 0.0 3.784296607113191E-5 0.0 1.110274781560939 0.0 54-55 0.0 3.784296607113191E-5 0.0 1.288969267348824 0.0 56-57 0.0 3.784296607113191E-5 0.0 1.5053175043774851 0.0 58-59 0.0 3.784296607113191E-5 0.0 1.7452797522345325 0.0 60-61 0.0 3.784296607113191E-5 0.0 2.0051474002449954 0.0 62-63 0.0 3.784296607113191E-5 0.0 2.2699346338447053 0.0 64-65 0.0 3.784296607113191E-5 0.0 2.545961228367542 0.0 66-67 0.0 3.784296607113191E-5 0.0 2.8617607802311373 0.0 68-69 0.0 3.784296607113191E-5 0.0 3.221117586042606 0.0 70-71 0.0 3.784296607113191E-5 0.0 3.6273807482992426 0.0 72-73 0.0 3.784296607113191E-5 0.0 4.037390364196922 0.0 74-75 0.0 3.784296607113191E-5 0.0 4.459282671440935 0.0 76-77 0.0 3.784296607113191E-5 0.0 4.917220403867702 0.0 78-79 0.0 3.784296607113191E-5 0.0 5.435271687898463 0.0 80-81 0.0 3.784296607113191E-5 0.0 5.999775212781538 0.0 82-83 0.0 3.784296607113191E-5 0.0 6.578223870661825 0.0 84-85 0.0 3.784296607113191E-5 0.0 7.185962984281167 0.0 86-87 0.0 3.784296607113191E-5 0.0 7.810409767420914 0.0 88-89 0.0 3.784296607113191E-5 0.0 8.479000370482638 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10925 0.0 24.684843 1 TACATGG 11550 0.0 23.007582 2 ACATGGG 11180 0.0 22.750341 3 TACGGTA 115 0.003745899 20.6318 5 GAGTACT 8115 0.0 20.349976 12-13 CATGGGG 5870 0.0 19.805824 4 AGTACTT 8435 0.0 19.521696 12-13 TTTGCGT 505 0.0 18.793322 3 GTACTTT 8815 0.0 18.626314 14-15 GTATCAA 21170 0.0 18.613155 1 GGTATCA 17050 0.0 18.276321 1 CATGGGA 6090 0.0 17.220268 4 AGTACAT 8415 0.0 17.086514 2 CCCTATA 335 1.4773832E-8 16.99814 2 CTCGCGC 615 0.0 16.976551 60-61 AGAGTAC 16865 0.0 16.629313 10-11 ATGGGGA 3335 0.0 16.078575 5 CAACGCA 24115 0.0 16.076803 5 CCTCGCG 620 0.0 16.07375 58-59 TACTTTT 10230 0.0 16.049948 14-15 >>END_MODULE