FastQCFastQC Report
Fri 27 May 2016
SRR939051_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939051_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3813054
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT145600.38184615271643146No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124420.3263001258308956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94880.24882941600092734No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT68890.18066882871315224No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT58930.15454803420040733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58310.15292204096768627No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC55520.14560507142044146No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40910.10728932766228855No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG157250.026.9851841
TACATGG160700.025.7324312
ACATGGG165250.024.135323
TTTGCGT6800.022.9872743
TAGGACG2955.456968E-1222.4795934
GAGTACT108800.021.7589712-13
CATGGGG68950.021.4339794
GTACTTT121750.019.62059614-15
CATGGGA98750.019.5226944
AGTACTT116850.019.26721812-13
CTTTGCG8550.018.2822782
AGTACAT117150.017.9119912
CGTATAC1350.00955649417.54363
GTATCAA349500.017.0984611
AGAGTAC226550.016.93793710-11
GAGTACA165200.016.0612831
TTGCGTT9850.015.8693894
ACTTTTT154200.015.81580516-17
CAACGCA385850.015.3452495
ATCAACG387900.015.2763633
Produced by FastQC (version 0.11.5)