FastQCFastQC Report
Fri 27 May 2016
SRR939051_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939051_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3813054
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT149860.3930182997670634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124160.32561825770104486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101670.26663666446895323No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT68750.1803016689509249No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63580.16674298344581534No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT57230.1500896656590754No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC52020.136426077364758No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40100.10516504618083038No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG157750.026.7301061
TACATGG162300.025.4674842
ACATGGG163900.024.1765273
GAGTACT125300.021.79862212-13
CATGGGG64100.020.2851814
CATGGGA100100.020.148434
GTACTTT135150.020.0695614-15
CGTATAG2401.3702811E-719.8525161
AGTACTT132000.019.39779312-13
AGTACAT120600.019.0451532
GTATCAA361450.016.8464891
GAGTACA163350.016.8008061
AGAGTAC240950.016.63430410-11
ATGGGGG35900.016.1269325
ACTTTTT172800.016.06650216-17
CTCCGAC8400.015.81984294-95
TACTTTT163100.015.69911414-15
GTATAGG10350.015.6518371
ATGGGAT46500.015.5123295
TACCTGG23350.015.4458952
Produced by FastQC (version 0.11.5)