##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939051_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3813054 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16064682010798 33.0 31.0 34.0 30.0 34.0 2 32.396340046587326 34.0 31.0 34.0 31.0 34.0 3 32.48969618578703 34.0 31.0 34.0 30.0 34.0 4 36.04107862096891 37.0 35.0 37.0 35.0 37.0 5 35.96451532026559 37.0 35.0 37.0 35.0 37.0 6 36.00764138142287 37.0 35.0 37.0 35.0 37.0 7 35.940561030606965 37.0 35.0 37.0 35.0 37.0 8 35.91446462599271 37.0 35.0 37.0 35.0 37.0 9 37.58770922205665 39.0 37.0 39.0 35.0 39.0 10-11 37.497853820061295 39.0 37.0 39.0 34.5 39.0 12-13 37.41264757855514 39.0 37.0 39.0 34.0 39.0 14-15 38.71591970635611 40.0 38.0 41.0 34.0 41.0 16-17 38.80765968695958 40.0 38.0 41.0 35.0 41.0 18-19 38.84971705095181 40.0 38.0 41.0 35.0 41.0 20-21 38.82358917550079 40.0 38.0 41.0 34.0 41.0 22-23 38.750303037932326 40.0 38.0 41.0 34.0 41.0 24-25 38.671716162425184 40.0 38.0 41.0 34.0 41.0 26-27 38.51071477088969 40.0 38.0 41.0 34.0 41.0 28-29 38.354672003071556 40.0 38.0 41.0 34.0 41.0 30-31 38.17117289710558 40.0 38.0 41.0 33.5 41.0 32-33 38.085471776691335 40.0 37.5 41.0 33.0 41.0 34-35 37.988725966115354 40.0 37.0 41.0 33.0 41.0 36-37 37.85490226469386 40.0 37.0 41.0 33.0 41.0 38-39 37.71582975221437 40.0 37.0 41.0 33.0 41.0 40-41 37.55925027550095 40.0 37.0 41.0 32.5 41.0 42-43 37.373270087441725 40.0 37.0 41.0 32.0 41.0 44-45 37.13335190112702 40.0 36.5 41.0 31.0 41.0 46-47 36.82324902820679 40.0 36.0 41.0 31.0 41.0 48-49 36.504073768690404 39.0 36.0 41.0 30.0 41.0 50-51 36.24961264645085 39.0 35.5 41.0 30.0 41.0 52-53 35.995776089192546 39.0 35.0 40.0 29.0 41.0 54-55 35.69997487578198 39.0 35.0 40.0 28.0 41.0 56-57 35.576552023653484 38.5 35.0 40.0 28.0 41.0 58-59 35.30789414469346 38.0 34.5 40.0 28.0 41.0 60-61 34.940762968476186 38.0 34.0 40.0 27.0 41.0 62-63 34.537058090444035 37.5 34.0 40.0 26.0 41.0 64-65 34.129009187910796 37.0 33.5 39.5 26.0 41.0 66-67 33.67701768713478 36.0 33.0 39.0 25.5 40.5 68-69 33.187878404029945 36.0 32.5 39.0 24.5 40.0 70-71 32.65143425715975 35.0 32.0 38.0 22.5 40.0 72-73 32.13766366802044 35.0 31.0 37.5 22.0 39.5 74-75 31.60577335122975 35.0 31.0 37.0 20.0 39.0 76-77 29.688624656246674 32.5 28.5 35.0 18.0 37.0 78-79 30.65986109821681 34.0 30.0 36.0 18.0 38.0 80-81 30.632111294516154 34.0 30.0 35.5 18.0 37.0 82-83 30.209915726344292 34.0 30.0 35.0 15.0 37.0 84-85 29.752826081141258 34.0 30.0 35.0 9.0 36.0 86-87 29.061418616153876 34.0 29.0 35.0 4.5 36.0 88-89 28.362752927181205 33.0 28.5 35.0 2.0 35.0 90-91 27.773536252043638 33.0 26.5 35.0 2.0 35.0 92-93 27.358912960582252 33.0 26.0 34.5 2.0 35.0 94-95 26.794840041604445 32.0 25.5 34.0 2.0 35.0 96-97 25.920643924790994 32.0 24.5 34.0 2.0 35.0 98-99 24.696760129806712 32.0 11.0 34.0 2.0 35.0 100-101 19.117215360705618 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.23125005820265443 1101 2 0.17037703179612151 1101 3 0.17889327882711825 1101 4 0.10874336128112105 1101 5 0.13490304473582881 1101 6 0.16238234800469797 1101 7 0.15814877803569516 1101 8 0.15018167366481094 1101 9 0.29276000058532503 1101 10-11 0.3168876520999788 1101 12-13 0.19348575653891942 1101 14-15 0.2851285917235771 1101 16-17 0.30480098816116197 1101 18-19 0.2517567109233383 1101 20-21 0.3713063535412573 1101 22-23 0.30421857891040105 1101 24-25 0.28988341545176866 1101 26-27 0.18445403025136642 1101 28-29 0.2261682841540278 1101 30-31 0.16419299575621693 1101 32-33 0.2684612317303774 1101 34-35 0.3389386346476684 1101 36-37 0.38053802167528517 1101 38-39 0.2937491816216351 1101 40-41 0.3076041110035348 1101 42-43 0.27435541307678335 1101 44-45 0.40751293377044107 1101 46-47 0.3941255863267372 1101 48-49 0.32605252030547405 1101 50-51 0.32881621332961686 1101 52-53 0.30450671519988504 1101 54-55 0.36924078921585846 1101 56-57 0.304910438197318 1101 58-59 0.17337260006421218 1101 60-61 0.08713004096139088 1101 62-63 0.14182139641518887 1101 64-65 0.1939900698472954 1101 66-67 0.07365596739671076 1101 68-69 0.16319054995104665 1101 70-71 0.22146761298672857 1101 72-73 0.3023150344432892 1101 74-75 0.3341325548557954 1101 76-77 0.08213405731472179 1101 78-79 0.36835731482787537 1101 80-81 0.4501028235536637 1101 82-83 0.24537836773891186 1101 84-85 0.4337221189832263 1101 86-87 0.4797763658403902 1101 88-89 0.5828056488924425 1101 90-91 0.6246157805218893 1101 92-93 0.48920739016437054 1101 94-95 0.49201187407691194 1101 96-97 0.7870826906258976 1101 98-99 1.011917055803277 1101 100-101 1.3321018673737264 1102 1 0.13307832525379837 1102 2 0.10043930901988318 1102 3 0.17969927826641907 1102 4 0.10139424838111921 1102 5 0.11984437198161402 1102 6 0.10538867585122347 1102 7 0.17017769389136106 1102 8 0.16064064278652523 1102 9 0.12394366833333237 1102 10-11 0.22753185090650163 1102 12-13 0.2626334997404953 1102 14-15 0.31664867662885854 1102 16-17 0.3172824651926831 1102 18-19 0.2219602633283202 1102 20-21 0.23346643203859685 1102 22-23 0.20253248815786407 1102 24-25 0.24614618128139654 1102 26-27 0.17023589976956544 1102 28-29 0.2467563133101791 1102 30-31 0.2470202014913241 1102 32-33 0.3138925665856007 1102 34-35 0.3392104717256501 1102 36-37 0.2962339846693993 1102 38-39 0.3149184314914635 1102 40-41 0.3992046957285922 1102 42-43 0.3091035007054401 1102 44-45 0.1993097618372346 1102 46-47 0.3410317499696518 1102 48-49 0.35499390762574734 1102 50-51 0.20785722418308694 1102 52-53 0.2409689283441807 1102 54-55 0.27489429956869316 1102 56-57 0.2878542885717792 1102 58-59 0.20788289282723582 1102 60-61 0.28355360618225944 1102 62-63 0.31042546546080274 1102 64-65 0.22199629756966743 1102 66-67 0.0457723917629167 1102 68-69 -0.004033808253119275 1102 70-71 0.061708011080362724 1102 72-73 0.12851001622069447 1102 74-75 0.12169463183124307 1102 76-77 -0.06450577607868624 1102 78-79 0.18051038463443447 1102 80-81 0.20645469988031095 1102 82-83 0.28961321895441827 1102 84-85 0.4402216784369486 1102 86-87 0.5471188705462247 1102 88-89 0.2853733328205159 1102 90-91 0.3130008780428142 1102 92-93 0.5101753904847719 1102 94-95 0.6767529530147947 1102 96-97 0.5831743442764257 1102 98-99 0.7573814316293017 1102 100-101 0.949709701087528 1103 1 0.05400691205582575 1103 2 0.08487367971468984 1103 3 0.11834354728810581 1103 4 0.09701107627076055 1103 5 0.11105075226549843 1103 6 0.09601334420599983 1103 7 0.14247595688863157 1103 8 0.1260515952369019 1103 9 0.08584847982989885 1103 10-11 0.13065449839502463 1103 12-13 0.08766231258199042 1103 14-15 0.14380572198850672 1103 16-17 0.08730292677341112 1103 18-19 0.15155291124332848 1103 20-21 0.2228204342464224 1103 22-23 0.1102076637872571 1103 24-25 0.09648132273211729 1103 26-27 0.13795150760150676 1103 28-29 0.1474379279401674 1103 30-31 0.07775628776520449 1103 32-33 0.1614866447284058 1103 34-35 0.08591893932880623 1103 36-37 0.03215995302386432 1103 38-39 0.05156596067872954 1103 40-41 0.06642381515658258 1103 42-43 0.22763853880683627 1103 44-45 0.11952472249605961 1103 46-47 0.09968130547826348 1103 48-49 0.018316598556701535 1103 50-51 0.024361576856229306 1103 52-53 0.01325672746958162 1103 54-55 0.11739579476175521 1103 56-57 0.24399752360422866 1103 58-59 0.11863809178462503 1103 60-61 0.07637687783330449 1103 62-63 0.18736896848739093 1103 64-65 0.16713771357454021 1103 66-67 0.16130283567904513 1103 68-69 0.2699636685155937 1103 70-71 0.09193285538687945 1103 72-73 0.12427227209261105 1103 74-75 0.23637655988146022 1103 76-77 0.004987350992394113 1103 78-79 0.33680529621719657 1103 80-81 0.23447196477297894 1103 82-83 0.16913078486937394 1103 84-85 0.3623626610093922 1103 86-87 0.5701026907223543 1103 88-89 0.4428475376406418 1103 90-91 0.34794624274594455 1103 92-93 0.4514819958162839 1103 94-95 0.6039596149801554 1103 96-97 0.3975500924928532 1103 98-99 0.6415623633800571 1103 100-101 0.8683848360516393 1104 1 0.044949144473207525 1104 2 0.06896554994829529 1104 3 0.0606834083292469 1104 4 0.041012635618670856 1104 5 0.08783895962416466 1104 6 0.1494183422200308 1104 7 0.11383110443853184 1104 8 0.10347896708001514 1104 9 0.14503950908441254 1104 10-11 0.18099977737787043 1104 12-13 0.142565579734395 1104 14-15 0.15201250988945958 1104 16-17 0.11801350289214696 1104 18-19 0.12764722171736054 1104 20-21 0.12383126972559921 1104 22-23 0.1047919857599382 1104 24-25 0.08574217096466441 1104 26-27 0.0673996651447979 1104 28-29 0.1805995373573026 1104 30-31 0.09713871226178128 1104 32-33 0.08558557924136778 1104 34-35 0.13097700377608135 1104 36-37 0.13401767740266024 1104 38-39 0.12359800330564497 1104 40-41 0.10610383515452071 1104 42-43 0.0725379924772298 1104 44-45 0.18900746412524683 1104 46-47 0.3541043018823373 1104 48-49 0.1996668686978822 1104 50-51 0.169477744960858 1104 52-53 0.20215881573297878 1104 54-55 0.3231924895230449 1104 56-57 0.222440153175107 1104 58-59 0.2081718097323133 1104 60-61 0.1597436205674967 1104 62-63 0.06576133641394932 1104 64-65 0.13695457346597806 1104 66-67 0.07339382922409499 1104 68-69 0.11347888561849118 1104 70-71 0.1317653095658713 1104 72-73 0.14516242465048634 1104 74-75 0.18310289537425817 1104 76-77 0.04732411565107597 1104 78-79 0.15296110424245413 1104 80-81 0.18782403324135188 1104 82-83 0.12358817294707336 1104 84-85 0.15111803086823983 1104 86-87 0.3672134020794431 1104 88-89 0.43949592594931275 1104 90-91 0.4547600789350206 1104 92-93 0.4356180885357226 1104 94-95 0.6867152466167141 1104 96-97 0.6534296417598178 1104 98-99 0.9767703824770599 1104 100-101 1.054468488664046 1105 1 0.04767278209250492 1105 2 0.08524497828773292 1105 3 0.041864705399383695 1105 4 0.10011631066696935 1105 5 0.08581552430124617 1105 6 0.06913657327169886 1105 7 0.08026265192332716 1105 8 0.058525979652380045 1105 9 0.017903440210282895 1105 10-11 0.10406825244728424 1105 12-13 0.10029564019698256 1105 14-15 0.08204956953019149 1105 16-17 0.00636164944645401 1105 18-19 0.04995616197257391 1105 20-21 0.04854588817349992 1105 22-23 0.07499983189209303 1105 24-25 0.06100927338610518 1105 26-27 0.049866395319519086 1105 28-29 0.04962265063432625 1105 30-31 0.06614509455463491 1105 32-33 0.2161693782105374 1105 34-35 0.25657797145408523 1105 36-37 0.24095719296954599 1105 38-39 0.22755195372755566 1105 40-41 0.21868060789620358 1105 42-43 0.2941849075713705 1105 44-45 0.1995787506129787 1105 46-47 0.2666936326854099 1105 48-49 0.3075032483172322 1105 50-51 0.39247366932768557 1105 52-53 0.3049712242292699 1105 54-55 0.3878760931742775 1105 56-57 0.38908421323564113 1105 58-59 0.3367160632247206 1105 60-61 0.38908317341563503 1105 62-63 0.35407387559894943 1105 64-65 0.20254659332877623 1105 66-67 0.35451711402317443 1105 68-69 0.3546940580199234 1105 70-71 0.573281678975313 1105 72-73 0.4769623987799392 1105 74-75 0.42817464214087053 1105 76-77 0.2667763169817903 1105 78-79 0.358946435043638 1105 80-81 0.4287171204450644 1105 82-83 0.36908550282659647 1105 84-85 0.4264700691641963 1105 86-87 0.5045696346736008 1105 88-89 0.6804338858254475 1105 90-91 0.5544260172131708 1105 92-93 0.7053371918932818 1105 94-95 0.721372470480425 1105 96-97 0.8016778314893322 1105 98-99 1.0949903849383773 1105 100-101 0.8373165815440338 1106 1 -0.009969435649111347 1106 2 -9.34027505195445E-4 1106 3 -0.03140799093058888 1106 4 -0.041941656079337974 1106 5 0.02650359124556445 1106 6 0.047044038424481016 1106 7 0.13276114469860545 1106 8 0.08290394393643652 1106 9 7.13534723381315E-4 1106 10-11 0.05066157756908751 1106 12-13 0.06391751176273175 1106 14-15 -0.1114118512501392 1106 16-17 -0.21238523879079452 1106 18-19 -0.11053302071030657 1106 20-21 -0.06520544780997284 1106 22-23 -0.1123807141366413 1106 24-25 0.014609533853857215 1106 26-27 -0.05653529651382172 1106 28-29 -0.0578812464241949 1106 30-31 0.001098541075158721 1106 32-33 0.07927610782558503 1106 34-35 -0.04793588746967714 1106 36-37 -0.08454661839290623 1106 38-39 0.022389550641051414 1106 40-41 0.13937124270486123 1106 42-43 0.20501385702819874 1106 44-45 0.16749206361663482 1106 46-47 0.22557151821357735 1106 48-49 0.19565495327655924 1106 50-51 0.08493185779746426 1106 52-53 -0.03248484899621218 1106 54-55 0.09027185628864487 1106 56-57 0.11783739892633349 1106 58-59 0.061197873632444555 1106 60-61 0.09572722819110879 1106 62-63 0.09515341361269236 1106 64-65 0.16624827689425814 1106 66-67 0.1780258517020954 1106 68-69 0.08630252177321296 1106 70-71 0.15965576400390802 1106 72-73 0.21617880185075222 1106 74-75 0.21821718238935617 1106 76-77 -0.03002829117297523 1106 78-79 0.21491029242849535 1106 80-81 0.2819316882925058 1106 82-83 0.1940725729808932 1106 84-85 0.21145231740636916 1106 86-87 0.45379803821031217 1106 88-89 0.4516343820177049 1106 90-91 0.62390334366507 1106 92-93 0.559483402648862 1106 94-95 0.7172127414730944 1106 96-97 0.6441266808990243 1106 98-99 0.6373009287522997 1106 100-101 0.9631770876697914 1107 1 7.228117044419946E-4 1107 2 0.027572573701284853 1107 3 0.07798932796853819 1107 4 0.04921290383777688 1107 5 0.0755610558112636 1107 6 0.006927231433429881 1107 7 0.03704961102001647 1107 8 0.014181859806555508 1107 9 -0.01917750232313864 1107 10-11 -0.020390722882574153 1107 12-13 0.007512700556787877 1107 14-15 0.08334861126846249 1107 16-17 0.08802263644633257 1107 18-19 0.09294929782826244 1107 20-21 0.12606460194741942 1107 22-23 0.061855044078619414 1107 24-25 0.06935547092200522 1107 26-27 0.13082252110714876 1107 28-29 0.07410152907002754 1107 30-31 0.10323331871214947 1107 32-33 0.19712938753101383 1107 34-35 0.22966703057116433 1107 36-37 0.2766633648741248 1107 38-39 0.17976993642641048 1107 40-41 0.24526873011098616 1107 42-43 0.16795795391163182 1107 44-45 0.13128439824063065 1107 46-47 0.1781592779829353 1107 48-49 0.23999628223018732 1107 50-51 0.06819333650892645 1107 52-53 -0.03330681730511742 1107 54-55 0.061969045533039946 1107 56-57 0.12276026834297227 1107 58-59 0.13495585196053383 1107 60-61 0.20480419983336873 1107 62-63 0.2095467442675769 1107 64-65 0.16900112201351902 1107 66-67 0.15761520235412974 1107 68-69 0.16211209120471182 1107 70-71 0.24645423404866307 1107 72-73 0.3323158488134297 1107 74-75 0.34262818052473776 1107 76-77 0.24334793418195844 1107 78-79 0.526125242604877 1107 80-81 0.49940130184488396 1107 82-83 0.3672325722156238 1107 84-85 0.5906983626427724 1107 86-87 0.5699794930217408 1107 88-89 0.6977126943575662 1107 90-91 0.6558265162471422 1107 92-93 0.8892515988292722 1107 94-95 1.0794473061584426 1107 96-97 1.1074948782567766 1107 98-99 1.0679910936831227 1107 100-101 0.9696838860809684 1108 1 0.04334457007447412 1108 2 0.08596392314217383 1108 3 0.05958738452797263 1108 4 0.06112511700655432 1108 5 0.06077684253080662 1108 6 0.10644442725302383 1108 7 0.13188937488851593 1108 8 0.12833680912476098 1108 9 0.12255323578209243 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-0.7515197233736544 2316 50-51 -0.7872154463645629 2316 52-53 -0.8280161431041861 2316 54-55 -0.8008951133494762 2316 56-57 -0.7876888132038715 2316 58-59 -0.9067602969109672 2316 60-61 -0.8289665370464476 2316 62-63 -0.7756410823814122 2316 64-65 -0.8045315631007881 2316 66-67 -0.7792913362117062 2316 68-69 -0.5975816920245123 2316 70-71 -0.5948767436831304 2316 72-73 -0.6371977983315169 2316 74-75 -0.8822623614379133 2316 76-77 -0.6921867036471632 2316 78-79 -0.6877986083750365 2316 80-81 -0.812460969184734 2316 82-83 -0.8073724773551625 2316 84-85 -1.0820512363992165 2316 86-87 -1.1708869934602681 2316 88-89 -1.2125949768057147 2316 90-91 -1.4967569163077634 2316 92-93 -1.428592882894101 2316 94-95 -1.2295434397334688 2316 96-97 -1.1947696594535628 2316 98-99 -1.2187860210935924 2316 100-101 -0.8796128765670304 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 28.0 5 69.0 6 182.0 7 440.0 8 1127.0 9 1968.0 10 3366.0 11 5454.0 12 8051.0 13 11106.0 14 13742.0 15 18026.0 16 23014.0 17 32327.0 18 31680.0 19 22618.0 20 21254.0 21 23168.0 22 24976.0 23 28369.0 24 32483.0 25 38713.0 26 46872.0 27 56085.0 28 68938.0 29 83413.0 30 100689.0 31 123469.0 32 152137.0 33 192443.0 34 261362.0 35 371044.0 36 580662.0 37 863724.0 38 544928.0 39 25120.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.8634388372611 19.932591641300334 14.159994018442784 23.04397550299578 2 15.583597819490624 21.716162425184642 39.029685915803974 23.67055383952076 3 19.586950512633706 25.301477503334596 30.23272159271807 24.878850391313627 4 12.916523080973938 16.932411657427355 37.43888756886212 32.71217769273658 5 13.149328595923373 38.17470195806301 34.54996965686822 14.125999789145393 6 31.336954577616787 36.57739963819028 17.44971353670837 14.635932247484561 7 27.733438865539277 33.406109643346255 21.3032650468627 17.557186444251773 8 25.637350008680706 35.1164447185904 20.24563512606955 19.000570146659346 9 26.185965370540252 17.047935854042457 21.058841548008502 35.70725722740879 10-11 24.814046693280503 26.803174043693062 27.506258762661112 20.876520500365324 12-13 25.883661757740644 24.343951069143003 27.977941041485384 21.79444613163097 14-15 22.564612221765596 25.57213880766857 26.81258698820383 25.050661982361998 16-17 21.63730497112349 28.90262974103328 27.48243425516285 21.977631032680375 18-19 21.42689578439208 27.885783821669495 29.511838674335976 21.175481719602445 20-21 22.430694661024994 26.798387853935456 29.15175080132618 21.61916668371337 22-23 22.04690780670822 26.82057479385291 29.106026298080227 22.026491101358648 24-25 22.166941337280477 26.96675786220592 29.22864676379971 21.637654036713897 26-27 22.00620841013639 26.781410226738227 29.558226759734683 21.654154603390694 28-29 21.514880198392156 27.43303661579406 29.479099955049154 21.57298323076463 30-31 21.902509117363667 26.942616076247543 29.198065382761428 21.95680942362736 32-33 21.649300009913315 27.243621517030707 29.16679123872885 21.94028723432713 34-35 22.264968602940012 27.035189283990178 29.09167999221516 21.608162120854647 36-37 22.200451111863387 26.729840340187188 29.32940622666581 21.740302321283618 38-39 21.878267524817943 27.177924754784417 29.317375255221844 21.6264324651758 40-41 22.060619333247658 27.10961207088735 29.14184027003706 21.687928325827933 42-43 21.701324550381198 27.266374338714883 28.72861732235884 22.30368378854508 44-45 21.928611254918067 27.101281883837895 28.679062738822264 22.29104412242177 46-47 22.219379612993105 27.434476254567592 28.303291863875412 22.04285226856389 48-49 22.17252867909431 27.385782911310493 28.298808880477733 22.142879529117472 50-51 21.722836766866045 27.641396044249312 28.49860124399723 22.137165944887414 52-53 22.17665944410963 27.848399209662382 27.869576460233713 22.105364885994273 54-55 21.742663492308264 27.340931966869604 28.210510525159098 22.705894015663038 56-57 21.957425727513957 27.656859829417574 27.989362332660384 22.39635211040809 58-59 22.350542587435807 27.27059820730307 27.977058116047466 22.401801089213656 60-61 22.086464131902733 27.320438558543863 28.52344412517049 22.06965318438292 62-63 21.915211119853133 27.275727773406764 28.516181484395485 22.29287962234461 64-65 21.63803193630677 27.577066724153802 28.245197319609854 22.539704019929573 66-67 21.74511919008493 27.49742128324499 27.826091289589172 22.93136823708091 68-69 21.572678914830437 27.518045399201803 28.11363372609546 22.795641959872302 70-71 21.917686431318945 27.337516682826074 27.903310024801648 22.841486861053333 72-73 21.92617780917868 27.08772285941925 28.061850160002976 22.924249171399094 74-75 21.5383259717801 27.852319951409026 27.64096705685259 22.968387019958282 76-77 21.728998334240675 27.265692550071215 27.864982673058115 23.14032644263 78-79 21.712362700119307 27.352486143796096 27.859290259179794 23.075860896904807 80-81 21.76528778582151 27.424126236151487 27.77138160482806 23.039204373198945 82-83 21.778972970222817 27.264156238018135 27.891107757718615 23.065763034040433 84-85 21.541888236056977 27.286928519473474 27.74451550604366 23.426667738425884 86-87 21.532179547857 27.190516352731702 27.908449482313774 23.36885461709752 88-89 21.567840628098164 27.0511488688312 27.66554004578829 23.71547045728235 90-91 21.467083469244354 26.877250429847404 27.83006238612104 23.825603714787196 92-93 21.65257822207606 27.136568220644136 27.60926018881453 23.601593368465277 94-95 21.524685022503874 26.87623073684166 27.604185721492243 23.994898519162223 96-97 21.21522538102004 26.7285357091717 27.90891500618664 24.14732390362161 98-99 21.237543449424003 26.920783183243667 27.412501894806628 24.429171472525695 100-101 21.352768572158773 26.043029451963452 28.271422312829436 24.33277966304834 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1095.0 1 777.5 2 535.5 3 634.0 4 597.0 5 617.5 6 1029.0 7 1839.0 8 2854.0 9 3422.0 10 3318.0 11 3211.5 12 3393.5 13 3788.0 14 4217.5 15 4495.5 16 4831.0 17 5162.5 18 5476.0 19 6043.5 20 6835.0 21 7961.0 22 9611.0 23 11785.5 24 14121.5 25 16969.0 26 21474.0 27 27173.5 28 31970.0 29 37252.5 30 43842.5 31 51500.5 32 59274.0 33 66706.0 34 75289.0 35 83333.0 36 93264.5 37 103407.0 38 108058.5 39 110541.5 40 113303.5 41 118074.0 42 122949.0 43 127239.0 44 133982.5 45 138789.0 46 140823.5 47 152226.5 48 180934.5 49 181679.5 50 156268.0 51 144041.5 52 134647.5 53 129224.0 54 123581.0 55 113025.5 56 101717.5 57 89603.5 58 78929.5 59 68746.0 60 57628.5 61 45655.5 62 35163.5 63 26725.5 64 19094.5 65 13365.0 66 9182.0 67 6760.0 68 4855.5 69 2947.0 70 1784.0 71 1062.0 72 651.5 73 435.5 74 302.5 75 203.5 76 134.0 77 69.0 78 29.0 79 16.5 80 10.5 81 7.5 82 10.0 83 8.0 84 3.0 85 0.0 86 1.5 87 2.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3858324587063283 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 9.703508001722504E-4 16-17 0.0024389898490816023 18-19 5.638524919919834E-4 20-21 0.0 22-23 0.0 24-25 5.245139460390542E-5 26-27 1.3112848650976356E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.013519346959156624 36-37 0.024062077274541613 38-39 0.011526193964208217 40-41 0.006399070141676463 42-43 0.0055860735253159275 44-45 0.0018882502057405953 46-47 0.0012326077731917777 48-49 0.003370002103300924 50-51 6.031910379449124E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 7.34319524454676E-4 60-61 0.0016391060813720445 62-63 0.0014161876543054464 64-65 7.080938271527232E-4 66-67 0.002911052400516751 68-69 0.0026619082761482004 70-71 2.622569730195271E-5 72-73 0.0 74-75 0.0 76-77 0.08864285688060017 78-79 0.0036978233195753326 80-81 2.6225697301952714E-4 82-83 0.0 84-85 2.2291842706659805E-4 86-87 0.09197352043794817 88-89 0.43141272061712216 90-91 0.383236114673435 92-93 0.0 94-95 0.001140817832634943 96-97 0.0 98-99 0.0 100-101 3.802726108783143E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3813054.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.81301253946277 #Duplication Level Percentage of deduplicated Percentage of total 1 69.04266572593751 29.55924513482499 2 15.114911275232885 12.942297719188254 3 6.062359892945284 7.786436703462078 4 3.020796016385606 5.173175109147045 5 1.7155660464314153 3.6724275329072373 6 1.1103165997918485 2.852159910579724 7 0.7277391341636854 2.180969327348535 8 0.5121411731945479 1.754104517596268 9 0.3997162022210471 1.5401749300124512 >10 2.038273295447987 15.638373160282104 >50 0.15439104329656367 4.576845022322829 >100 0.09352618150097927 7.417540491593162 >500 0.005024096000969651 1.4973352391955796 >1k 0.0021444312080507342 1.7897355665444803 >5k 2.4507785234865537E-4 0.6336958158053255 >10k+ 1.8380838926149154E-4 0.9854838191899279 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14986 0.3930182997670634 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12416 0.32561825770104486 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10167 0.26663666446895323 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6875 0.1803016689509249 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6358 0.16674298344581534 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5723 0.1500896656590754 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5202 0.136426077364758 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4010 0.10516504618083038 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.6225697301952714E-5 0.0 5 0.0 0.0 0.0 2.6225697301952714E-5 0.0 6 0.0 0.0 0.0 2.6225697301952714E-5 0.0 7 0.0 0.0 0.0 2.6225697301952714E-5 0.0 8 0.0 0.0 0.0 2.6225697301952714E-5 0.0 9 0.0 0.0 0.0 5.245139460390543E-5 0.0 10-11 1.3112848650976357E-5 0.0 0.0 1.180156378587872E-4 0.0 12-13 2.6225697301952714E-5 0.0 0.0 1.9669272976464535E-4 0.0 14-15 2.6225697301952714E-5 0.0 0.0 2.0980557841562172E-4 0.0 16-17 2.6225697301952714E-5 0.0 0.0 2.6225697301952714E-4 0.0 18-19 3.933854595292907E-5 0.0 0.0 3.933854595292907E-4 0.0 20-21 5.245139460390543E-5 0.0 0.0 6.163038865958887E-4 0.0 22-23 5.245139460390543E-5 0.0 0.0 8.916737082663922E-4 0.0 24-25 7.867709190585813E-5 0.0 0.0 0.0012981720164466594 0.0 26-27 7.867709190585813E-5 0.0 0.0 0.0021242814814581698 0.0 28-29 7.867709190585813E-5 0.0 0.0 0.005940120438892289 0.0 30-31 7.867709190585813E-5 0.0 0.0 0.01705981609492024 0.0 32-33 7.867709190585813E-5 0.0 0.0 0.03962702862325055 0.0 34-35 7.867709190585813E-5 0.0 0.0 0.07584471659724724 0.0 36-37 7.867709190585813E-5 0.0 0.0 0.13434113442925277 0.0 38-39 1.0490278920781086E-4 0.0 0.0 0.22294465276390002 0.0 40-41 1.180156378587872E-4 0.0 0.0 0.3197830400513604 0.0 42-43 1.3112848650976357E-4 0.0 0.0 0.41690991000914224 0.0 44-45 1.5735418381171627E-4 0.0 0.0 0.5404853956959434 0.0 46-47 1.5735418381171627E-4 0.0 0.0 0.6890670837601566 0.0 48-49 1.5735418381171627E-4 0.0 0.0 0.8476669881937156 0.0 50-51 1.5735418381171627E-4 0.0 0.0 1.0196419982512706 0.0 52-53 1.5735418381171627E-4 0.0 0.0 1.1917612496439862 0.0 54-55 1.5735418381171627E-4 0.0 0.0 1.3856740554946245 0.0 56-57 1.83579881113669E-4 0.0 0.0 1.6213381714499717 0.0 58-59 1.83579881113669E-4 0.0 0.0 1.8804742864905664 0.0 60-61 1.83579881113669E-4 0.0 0.0 2.167540769157741 0.0 62-63 1.83579881113669E-4 0.0 0.0 2.4575969813173377 0.0 64-65 1.83579881113669E-4 0.0 0.0 2.765801376009886 0.0 66-67 1.83579881113669E-4 0.0 0.0 3.112911592649881 0.0 68-69 1.83579881113669E-4 0.0 0.0 3.5133124262074444 0.0 70-71 1.9669272976464535E-4 0.0 0.0 3.945774174716645 0.0 72-73 2.0980557841562172E-4 0.0 0.0 4.392686282439221 0.0 74-75 2.0980557841562172E-4 0.0 0.0 4.8591627603490535 0.0 76-77 2.0980557841562172E-4 0.0 0.0 5.356257739858917 0.0 78-79 2.0980557841562172E-4 0.0 0.0 5.924725954576044 0.0 80-81 2.0980557841562172E-4 0.0 0.0 6.536046958684561 0.0 82-83 2.360312757175744E-4 0.0 0.0 7.154475126761908 0.0 84-85 2.360312757175744E-4 0.0 0.0 7.792284085145399 0.0 86-87 2.360312757175744E-4 0.0 0.0 8.464120885778172 0.0 88-89 2.360312757175744E-4 0.0 0.0 9.176777984261435 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 15775 0.0 26.730106 1 TACATGG 16230 0.0 25.467484 2 ACATGGG 16390 0.0 24.176527 3 GAGTACT 12530 0.0 21.798622 12-13 CATGGGG 6410 0.0 20.285181 4 CATGGGA 10010 0.0 20.14843 4 GTACTTT 13515 0.0 20.06956 14-15 CGTATAG 240 1.3702811E-7 19.852516 1 AGTACTT 13200 0.0 19.397793 12-13 AGTACAT 12060 0.0 19.045153 2 GTATCAA 36145 0.0 16.846489 1 GAGTACA 16335 0.0 16.800806 1 AGAGTAC 24095 0.0 16.634304 10-11 ATGGGGG 3590 0.0 16.126932 5 ACTTTTT 17280 0.0 16.066502 16-17 CTCCGAC 840 0.0 15.819842 94-95 TACTTTT 16310 0.0 15.699114 14-15 GTATAGG 1035 0.0 15.651837 1 ATGGGAT 4650 0.0 15.512329 5 TACCTGG 2335 0.0 15.445895 2 >>END_MODULE