FastQCFastQC Report
Fri 27 May 2016
SRR939050_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939050_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3828184
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT143250.3741983143965912No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123800.32339093418707143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95990.25074552320369137No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT68420.17872704133343642No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58690.15331029020548645No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT58260.15218704221113719No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC54820.14320105825634297No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40420.10558531146883222No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG162850.026.900521
TACATGG167700.025.8557342
ACATGGG167950.024.7462253
CATGGGG70950.023.3512484
GAGTACT110850.021.6071712-13
AGTACAT119950.020.2052482
CATGGGA103050.019.7521724
GTACTTT123150.019.37246514-15
AGTACTT121100.018.5275212-13
GTATCAA351200.017.5630231
ATGGGGA40600.017.4887875
GAGTACA168400.017.2581751
AGAGTAC230000.016.3921310-11
ATGGGAT47500.016.3434855
CAACGCA387000.015.7910025
ATCAACG387000.015.766543
TCAACGC389950.015.6715434
AACGCAG390450.015.6621656
TATCAAC391750.015.6357862
GTATAGA12250.015.4810491
Produced by FastQC (version 0.11.5)