FastQCFastQC Report
Fri 27 May 2016
SRR939050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939050_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3828184
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT149320.3900543965493821No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125500.32783168207170815No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103170.2695011525046863No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT66250.1730585572689296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63950.16705048660147997No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT56510.1476156840945994No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC52890.13815950330496132No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41460.10830200429237466No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG155250.027.3144551
TACATGG161700.025.6198392
ACATGGG164550.024.1090763
GAGTACT125050.021.74503512-13
CATGGGA97000.020.6938254
TTTGCGT7300.020.1516693
GACCGTA1656.375055E-520.131977
GTACTTT136250.019.62666714-15
CATGGGG68300.019.5235064
AGTACTT135350.019.12606812-13
ACCGTAC1801.2434881E-418.4543068
TAGGACG3409.0403773E-1018.1441484
CTTTGCG8500.017.8650072
GTATAGG10200.017.7507861
AGTACAT119200.017.476742
GTATCAA361750.017.0567151
AGAGTAC242450.016.97995210-11
CCGTCCG1700.001800080116.7486639
TACTTTT160850.015.99069814-15
ACTTTTT168750.015.95878816-17
Produced by FastQC (version 0.11.5)