##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939050_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3828184 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13634297619968 33.0 31.0 34.0 30.0 34.0 2 32.36109758569599 34.0 31.0 34.0 30.0 34.0 3 32.461521180800084 34.0 31.0 34.0 30.0 34.0 4 36.03849135778218 37.0 35.0 37.0 35.0 37.0 5 35.95143911577918 37.0 35.0 37.0 35.0 37.0 6 35.995383450743226 37.0 35.0 37.0 35.0 37.0 7 35.936449240684354 37.0 35.0 37.0 35.0 37.0 8 35.90390535042203 37.0 35.0 37.0 35.0 37.0 9 37.579265259977056 39.0 37.0 39.0 35.0 39.0 10-11 37.49869559561401 39.0 37.0 39.0 34.5 39.0 12-13 37.42826324439996 39.0 37.0 39.0 34.0 39.0 14-15 38.68056172326095 40.0 38.0 41.0 34.0 41.0 16-17 38.67466636922363 40.0 38.0 41.0 34.0 41.0 18-19 38.778484654865075 40.0 38.0 41.0 34.0 41.0 20-21 38.776405731803905 40.0 38.0 41.0 34.0 41.0 22-23 38.717732480988374 40.0 38.0 41.0 34.0 41.0 24-25 38.65138979735561 40.0 38.0 41.0 34.0 41.0 26-27 38.497056175983175 40.0 38.0 41.0 34.0 41.0 28-29 38.32254183706948 40.0 38.0 41.0 34.0 41.0 30-31 38.16541772809248 40.0 38.0 41.0 33.0 41.0 32-33 38.01962131391804 40.0 37.0 41.0 33.0 41.0 34-35 37.98974448459113 40.0 37.0 41.0 33.0 41.0 36-37 37.88516696167164 40.0 37.0 41.0 33.0 41.0 38-39 37.758792942032045 40.0 37.0 41.0 33.0 41.0 40-41 37.61719512437229 40.0 37.0 41.0 33.0 41.0 42-43 37.45692291697578 40.0 37.0 41.0 32.0 41.0 44-45 37.2169309259952 40.0 37.0 41.0 31.5 41.0 46-47 36.92185655130474 40.0 36.0 41.0 31.0 41.0 48-49 36.64237233633493 40.0 36.0 41.0 30.0 41.0 50-51 36.38657781339664 39.0 36.0 41.0 30.0 41.0 52-53 36.17205037688888 39.0 35.0 41.0 29.5 41.0 54-55 35.932151641613885 39.0 35.0 40.0 29.0 41.0 56-57 35.66009483870158 38.5 35.0 40.0 28.0 41.0 58-59 35.36568122117433 38.0 34.5 40.0 28.0 41.0 60-61 34.958572785425154 38.0 34.0 40.0 27.0 41.0 62-63 34.65973931765035 37.5 34.0 40.0 26.0 41.0 64-65 34.40677146135087 37.0 34.0 40.0 26.0 41.0 66-67 33.959193445247145 36.5 33.5 39.0 26.0 41.0 68-69 33.545132626853885 36.0 33.0 39.0 25.5 40.0 70-71 33.07882262190114 35.5 32.5 38.5 25.0 40.0 72-73 32.561291855354916 35.0 32.0 37.5 23.5 39.5 74-75 32.09390875673688 35.0 31.5 37.0 22.0 39.0 76-77 30.086992422516786 33.0 29.5 35.0 20.0 37.5 78-79 31.078705986964053 34.0 31.0 36.0 20.0 38.0 80-81 31.07453821446409 34.0 31.0 36.0 20.0 37.0 82-83 30.74824068017629 34.0 31.0 35.0 19.5 37.0 84-85 30.346293307740694 34.0 30.5 35.0 17.5 36.5 86-87 29.784971542642673 34.0 30.0 35.0 10.5 36.0 88-89 29.217893262183843 34.0 29.0 35.0 4.5 35.5 90-91 28.750896378021537 34.0 29.0 35.0 2.0 35.0 92-93 28.39705850084531 33.0 29.0 35.0 2.0 35.0 94-95 28.006442872129448 33.0 28.5 35.0 2.0 35.0 96-97 27.383479738695947 33.0 27.0 35.0 2.0 35.0 98-99 26.49652446172911 33.0 25.5 34.5 2.0 35.0 100-101 22.186420637043568 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17424368637535537 1101 2 0.1848547508984879 1101 3 0.19641088882689672 1101 4 0.10653911082701484 1101 5 0.10248920974510867 1101 6 0.13480550995681284 1101 7 0.11580019124965446 1101 8 0.14095667666222766 1101 9 0.20174460390389726 1101 10-11 0.2543025984626084 1101 12-13 0.23257213484075834 1101 14-15 0.3539509462641419 1101 16-17 0.3191970889019444 1101 18-19 0.3029288349625361 1101 20-21 0.21472647498486452 1101 22-23 0.29096887882503353 1101 24-25 0.32116601613049056 1101 26-27 0.32443011021379675 1101 28-29 0.3346864439532027 1101 30-31 0.27617330588881117 1101 32-33 0.3051574923794931 1101 34-35 0.4666022750228862 1101 36-37 0.4271541515760262 1101 38-39 0.5070467678755577 1101 40-41 0.5431609048981443 1101 42-43 0.508617544885908 1101 44-45 0.5417940360573681 1101 46-47 0.49397236595296334 1101 48-49 0.3551239814502978 1101 50-51 0.4497504965993997 1101 52-53 0.5260005869645852 1101 54-55 0.5155257091266847 1101 56-57 0.40950487131849655 1101 58-59 0.42765622275668846 1101 60-61 0.4619983004467656 1101 62-63 0.33192543009564446 1101 64-65 0.3579899209732318 1101 66-67 0.43831376214610174 1101 68-69 0.3693060610643286 1101 70-71 0.3244041799127828 1101 72-73 0.27629726710402736 1101 74-75 0.22891719305532376 1101 76-77 0.20193979618935032 1101 78-79 0.30330850609430726 1101 80-81 0.5065057890192541 1101 82-83 0.4194723905058346 1101 84-85 0.4220000924180205 1101 86-87 0.48134794287624416 1101 88-89 0.4762096211725506 1101 90-91 0.5010946727131014 1101 92-93 0.5222597194823777 1101 94-95 0.5358080229175641 1101 96-97 0.48299011601807607 1101 98-99 0.5097612129890656 1101 100-101 1.0298691850180113 1102 1 0.12587101853705462 1102 2 0.15470411040961807 1102 3 0.1386676463452332 1102 4 0.0981371534479365 1102 5 0.1011074692637095 1102 6 0.10612813976427304 1102 7 0.12955676271066352 1102 8 0.1719135789095887 1102 9 0.2337209644306455 1102 10-11 0.22806975380789396 1102 12-13 0.22327490395739602 1102 14-15 0.21646912732290957 1102 16-17 0.190155837733343 1102 18-19 0.12274232198068802 1102 20-21 0.20303999617681256 1102 22-23 0.1605004819917255 1102 24-25 0.22256406410729568 1102 26-27 0.18038808164131126 1102 28-29 0.2543289820549859 1102 30-31 0.26902123010052037 1102 32-33 0.18837368220513895 1102 34-35 0.15932185764693685 1102 36-37 0.1444434941081667 1102 38-39 0.22680625629983098 1102 40-41 0.26803079466667157 1102 42-43 0.17147674445167382 1102 44-45 0.10812405419945748 1102 46-47 0.3193066596165792 1102 48-49 0.24021541334033003 1102 50-51 0.25745339002072143 1102 52-53 0.2984175376468059 1102 54-55 0.24925796323423555 1102 56-57 0.07721235322136266 1102 58-59 0.07001722034373614 1102 60-61 0.029426996122914773 1102 62-63 0.09426538781605842 1102 64-65 0.1618307758057469 1102 66-67 0.17640511369773293 1102 68-69 0.17639786307054806 1102 70-71 0.13591843078521748 1102 72-73 0.22405420708244606 1102 74-75 0.295009913157557 1102 76-77 0.08909504554414482 1102 78-79 0.0857539669149503 1102 80-81 0.3660965348353642 1102 82-83 0.3280006510356621 1102 84-85 0.33597565595589884 1102 86-87 0.38767229336428954 1102 88-89 0.4779221480069751 1102 90-91 0.5991799409294352 1102 92-93 0.47698435663060934 1102 94-95 0.5691686020254991 1102 96-97 0.28716799242245017 1102 98-99 0.44710186018079767 1102 100-101 1.0738222418140602 1103 1 0.10688948099551965 1103 2 0.10288805861613781 1103 3 0.08316184599120646 1103 4 0.081059585101805 1103 5 0.08966233603171503 1103 6 0.0872241936895577 1103 7 0.12615300532428364 1103 8 0.1394064379474429 1103 9 0.06029298518284065 1103 10-11 0.1538834551285575 1103 12-13 0.1807962798186864 1103 14-15 0.280039698525421 1103 16-17 0.212067981464088 1103 18-19 0.25254293150477025 1103 20-21 0.21365693846953349 1103 22-23 0.20560291252483154 1103 24-25 0.18606684074537583 1103 26-27 0.11909697530847296 1103 28-29 0.27546097885570475 1103 30-31 0.3043944744466316 1103 32-33 0.18594322747603087 1103 34-35 0.22723559161087792 1103 36-37 0.17384893577800398 1103 38-39 0.24015747485814387 1103 40-41 0.21299226736837795 1103 42-43 0.11269446806694816 1103 44-45 0.20813467404163077 1103 46-47 0.26528305221685855 1103 48-49 0.2700621466807931 1103 50-51 0.14933865497010856 1103 52-53 0.11924116714833133 1103 54-55 0.2463707377287392 1103 56-57 0.1797190278509575 1103 58-59 0.11210612370591377 1103 60-61 0.013892439901049158 1103 62-63 0.18594958114108096 1103 64-65 0.17057466792696374 1103 66-67 0.19955840739671515 1103 68-69 0.21723225348964803 1103 70-71 0.09534169889860777 1103 72-73 0.23241402144290646 1103 74-75 0.16434627490934872 1103 76-77 0.1819301789603216 1103 78-79 0.3919568952482564 1103 80-81 0.3239621346585473 1103 82-83 0.2718764310160431 1103 84-85 0.25956745900524325 1103 86-87 0.23531075004650148 1103 88-89 0.44139024361063406 1103 90-91 0.5560241157108727 1103 92-93 0.557951718446283 1103 94-95 0.5616289451443706 1103 96-97 0.38582405335447945 1103 98-99 0.551923621217103 1103 100-101 0.9542169942796477 1104 1 -0.011865241902626167 1104 2 -0.044723514221004734 1104 3 -0.004647862916534962 1104 4 0.02714961844281305 1104 5 0.02962742701787846 1104 6 -0.0061809846861962114 1104 7 -0.048039492687976804 1104 8 -0.010292528798728995 1104 9 0.01854888069977534 1104 10-11 -0.02270407016677467 1104 12-13 -0.003679197769706377 1104 14-15 0.005908442235288192 1104 16-17 -0.028309975151017852 1104 18-19 -0.056901085767364634 1104 20-21 -0.02780587921034794 1104 22-23 -0.047907126897221985 1104 24-25 0.03008720885494398 1104 26-27 -0.04366757245005459 1104 28-29 -0.036508566325927916 1104 30-31 -0.010691066807886784 1104 32-33 -0.003713883572032728 1104 34-35 0.021238793246780574 1104 36-37 0.010785649789944785 1104 38-39 0.10677539921352519 1104 40-41 0.09426480751051258 1104 42-43 0.05730421073774039 1104 44-45 0.09068222340236787 1104 46-47 0.05515913784807225 1104 48-49 0.01831534823968184 1104 50-51 -0.016111071213472883 1104 52-53 -0.08829002438823608 1104 54-55 -0.022172634995833107 1104 56-57 -0.10755301385503202 1104 58-59 -0.03724219721426181 1104 60-61 -0.07254152516468082 1104 62-63 -0.03786808087300386 1104 64-65 -0.024063724040395584 1104 66-67 0.045725778435603104 1104 68-69 0.12276177566558744 1104 70-71 -0.15915007952342108 1104 72-73 -0.0876494891186681 1104 74-75 0.026431295239568442 1104 76-77 -0.05253774353131746 1104 78-79 -0.014080304785135667 1104 80-81 0.1726440590780065 1104 82-83 0.24554237498109543 1104 84-85 0.11592543104168485 1104 86-87 -0.05034006493356813 1104 88-89 0.10123676039527041 1104 90-91 0.2721275310458857 1104 92-93 0.3670807859917744 1104 94-95 0.3271123881613818 1104 96-97 0.27969974251638874 1104 98-99 0.35082481287381384 1104 100-101 0.7536589799161852 1105 1 -0.0077555470167922635 1105 2 0.0015620395877959936 1105 3 -0.011101560869263949 1105 4 -0.005357060665772906 1105 5 0.063650612960096 1105 6 0.03465533777730201 1105 7 0.008197561431373401 1105 8 0.06600785426560662 1105 9 -0.04610801074353077 1105 10-11 -0.008825714639087323 1105 12-13 -0.020852827846645994 1105 14-15 0.023761498874719678 1105 16-17 0.006528894140529928 1105 18-19 0.02083965189151371 1105 20-21 0.11098599709167445 1105 22-23 0.02848530750784306 1105 24-25 0.07317213977557913 1105 26-27 0.036148072265945075 1105 28-29 0.003203749088470431 1105 30-31 -0.02822324391497233 1105 32-33 -0.01570403504440776 1105 34-35 0.1479370253261152 1105 36-37 0.1266941868645688 1105 38-39 0.1828070577424299 1105 40-41 0.15227186255112457 1105 42-43 0.10283299702706472 1105 44-45 0.13752357711428687 1105 46-47 0.21381337547871482 1105 48-49 0.16951097554096606 1105 50-51 0.15252111570053728 1105 52-53 0.22425555551292575 1105 54-55 0.23150979010750206 1105 56-57 0.19080209778044122 1105 58-59 0.17017599670722916 1105 60-61 0.10556801221603251 1105 62-63 0.1654238504072154 1105 64-65 0.1203249332136096 1105 66-67 0.23608007579932888 1105 68-69 0.13873346337751258 1105 70-71 0.13985570656085855 1105 72-73 0.12533197876960145 1105 74-75 0.13787575091079418 1105 76-77 -0.02381876535320515 1105 78-79 0.30572009462407124 1105 80-81 0.08799954515960806 1105 82-83 0.1231018331634921 1105 84-85 -0.01987563107774193 1105 86-87 0.04723554495299709 1105 88-89 0.20376129998997783 1105 90-91 0.3414993233649888 1105 92-93 0.4373023630043491 1105 94-95 0.4927873421728606 1105 96-97 0.3121979520100844 1105 98-99 0.47638135572560714 1105 100-101 1.0760316905198444 1106 1 -0.03581588512722789 1106 2 -0.10840941899684964 1106 3 -0.08341956245072879 1106 4 -0.04985583993587284 1106 5 -0.00455413490001888 1106 6 -0.009568272235568998 1106 7 0.033615149176746684 1106 8 -0.033226808691736665 1106 9 -0.06416225963391042 1106 10-11 -8.93279008778336E-4 1106 12-13 0.10204516473085334 1106 14-15 0.03136400555555241 1106 16-17 -0.06803800647531233 1106 18-19 -0.1327708082919159 1106 20-21 -0.11481974235897496 1106 22-23 0.021143901388605002 1106 24-25 -0.04180633151273838 1106 26-27 0.02823511636805165 1106 28-29 -0.005900212079041012 1106 30-31 0.07746310642008325 1106 32-33 0.09547523909748179 1106 34-35 0.13703515700333213 1106 36-37 -0.0059198633613917195 1106 38-39 0.13295236211685335 1106 40-41 0.2377640071686642 1106 42-43 0.28561175921272053 1106 44-45 0.2477577234979833 1106 46-47 0.2592073566742812 1106 48-49 0.09076163208009547 1106 50-51 0.046844433084530124 1106 52-53 0.10617504505736264 1106 54-55 0.07243577001019474 1106 56-57 0.1056328838381475 1106 58-59 0.1869258249969974 1106 60-61 0.1992077352863859 1106 62-63 0.11469501982605834 1106 64-65 0.08321041547406338 1106 66-67 0.164742986965976 1106 68-69 0.09691091075337965 1106 70-71 0.12082732675512631 1106 72-73 0.2013462235043022 1106 74-75 0.21351858208067398 1106 76-77 0.08266986136894516 1106 78-79 0.2705093960664371 1106 80-81 0.32060991588056 1106 82-83 0.38107445476030577 1106 84-85 0.24236896798607788 1106 86-87 0.25538535996768985 1106 88-89 0.4164478003911185 1106 90-91 0.6097192221280956 1106 92-93 0.5863689952327711 1106 94-95 0.6423401019459156 1106 96-97 0.6000579663240799 1106 98-99 0.5950993584614288 1106 100-101 1.0824451399042836 1107 1 5.099883480852441E-6 1107 2 -0.03181370165209785 1107 3 -0.06889850606243897 1107 4 -0.09280216399012176 1107 5 -0.1585495667344219 1107 6 -0.09491159843114616 1107 7 -0.1066085481822796 1107 8 -0.04695243324562881 1107 9 0.004763507675157541 1107 10-11 0.07729144618750894 1107 12-13 -0.009910509787353305 1107 14-15 0.061926817832492986 1107 16-17 -0.015627799480313342 1107 18-19 -0.038068524209116106 1107 20-21 0.026899743722395897 1107 22-23 0.04377659232365971 1107 24-25 -0.04725229867904801 1107 26-27 -0.04702641111231287 1107 28-29 0.047239687992330914 1107 30-31 -0.05232818551424856 1107 32-33 3.5767291620913966E-5 1107 34-35 -0.06206477161907742 1107 36-37 0.052706046702844844 1107 38-39 -3.5484701947297026E-4 1107 40-41 -0.13491843394483283 1107 42-43 -0.09344948275730047 1107 44-45 -0.10584677329002545 1107 46-47 -0.18999507016369677 1107 48-49 -0.2027280181697293 1107 50-51 -0.14555399660854107 1107 52-53 -0.13451317764072002 1107 54-55 -0.34299299515753745 1107 56-57 -0.2611741825430087 1107 58-59 -0.2402743177581783 1107 60-61 -0.2099856265979767 1107 62-63 -0.2302778566907122 1107 64-65 -0.24668423120687777 1107 66-67 -0.19231597092124986 1107 68-69 -0.23755332907531823 1107 70-71 -0.20637609936906642 1107 72-73 -0.17299039319804876 1107 74-75 -0.10928255996072522 1107 76-77 -0.47030429994440226 1107 78-79 -0.1770069494010862 1107 80-81 -0.06623333893742611 1107 82-83 0.00424469030634711 1107 84-85 -0.09073888354854276 1107 86-87 -0.14020129242346258 1107 88-89 0.09367792173301481 1107 90-91 0.25355862255636197 1107 92-93 0.26910254269886735 1107 94-95 0.21220441315219674 1107 96-97 0.21744718830837684 1107 98-99 0.35725538986756433 1107 100-101 0.8297283661712349 1108 1 0.035810247539942 1108 2 0.024374431645114214 1108 3 0.06933756365670263 1108 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1992.0 10 3431.0 11 5272.0 12 7836.0 13 10924.0 14 13169.0 15 17080.0 16 22205.0 17 31242.0 18 30089.0 19 20692.0 20 18960.0 21 20240.0 22 22042.0 23 24544.0 24 28636.0 25 34417.0 26 41970.0 27 51108.0 28 62960.0 29 76661.0 30 93496.0 31 113620.0 32 141084.0 33 180616.0 34 241160.0 35 344215.0 36 542379.0 37 881127.0 38 693465.0 39 49753.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.85948084831162 19.949921278263698 14.156929938834928 23.03366793458975 2 15.615576471768335 21.679025877544024 39.06747951509123 23.637918135596408 3 19.58769484434395 25.324592548320563 30.23890701178418 24.84880559555131 4 12.927748509476034 16.920189834135453 37.48858466573184 32.66347699065666 5 13.150676772806207 38.223400853881024 34.4968663862192 14.129055987093567 6 31.3644798682613 36.53870869320806 17.434637415547424 14.662174022983221 7 27.68351782463957 33.45424357867856 21.28476583152743 17.577472765154447 8 25.67394879660957 35.10894460663333 20.222199350919393 18.994907245837712 9 26.20461816882365 17.031391385576033 21.104105758761857 35.65988468683846 10-11 24.792172998999003 26.77846728370423 27.546390142166626 20.88296957513014 12-13 25.868309882701563 24.33306497284352 27.991614300671024 21.807010843783896 14-15 22.573619082610602 25.573618168327155 26.816103248200918 25.03665950086132 16-17 21.61590535032368 28.923442479426125 27.460756922077834 21.99989524817236 18-19 21.453710166491476 27.88335147944822 29.49108506801136 21.171853286048947 20-21 22.45427597001607 26.829339969029704 29.091430819417248 21.62495324153698 22-23 22.077269535633608 26.83034566781534 29.07356333969318 22.01882145685787 24-25 22.185062160021566 26.974160594161617 29.20203156379108 21.638745682025736 26-27 22.003043740844223 26.823149044037592 29.500384516522715 21.67342269859547 28-29 21.568281461915102 27.474345538249988 29.418152314517798 21.53922068531711 30-31 21.87234208178081 26.972214501706294 29.206550677814857 21.948892738698035 32-33 21.672547610041732 27.2344276032709 29.15098908516414 21.94203570152323 34-35 22.274571883477595 27.057212380538154 29.084259705520093 21.58395603046416 36-37 22.23611433124451 26.737862999742028 29.29727364457782 21.728749024435643 38-39 21.87101548381113 27.161270812882123 29.366428861139465 21.601284842167274 40-41 22.023618812763797 27.111844867907447 29.180564471731024 21.68397184759773 42-43 21.682563341804766 27.290298025761206 28.766307773527693 22.26083085890633 44-45 21.889485547790603 27.08796293998515 28.720388258721073 22.302163253503178 46-47 22.190318412903906 27.434267616884707 28.324757443104726 22.050656527106657 48-49 22.197243672472002 27.37087281963579 28.310002145931996 22.121881361960213 50-51 21.69905841224306 27.64666537710073 28.52570352635332 22.128572684302895 52-53 22.14650601956437 27.839113793903326 27.894988851110604 22.119391335421703 54-55 21.711717618588867 27.39166926145661 28.207264854562897 22.689348265391633 56-57 21.93764719773135 27.678633001966467 27.991757971926113 22.39196182837607 58-59 22.362775070585172 27.2701245619161 27.9501350055307 22.41696536196803 60-61 22.094894819272685 27.31453175941129 28.479508706861612 22.111064714454407 62-63 21.908952129782687 27.319480986284884 28.485895662277468 22.28567122165497 64-65 21.678535678610388 27.54369182608114 28.287361918574277 22.49041057673419 66-67 21.7277120717595 27.473469030037062 27.868793576377755 22.930025321825685 68-69 21.591713841706593 27.472029996977625 28.12757388158519 22.808682279730593 70-71 21.865406678466865 27.35347882964873 27.948317008795815 22.832797483088587 72-73 21.916396913001044 27.10368676113792 28.055874534766357 22.92404179109468 74-75 21.541663096653664 27.862440781320856 27.636654873433457 22.959241248592022 76-77 21.704222344363014 27.256096675874304 27.893674218602982 23.146006761159697 78-79 21.679462554428564 27.335892772106245 27.902780509709174 23.081864163756016 80-81 21.731021810863847 27.46951295966965 27.755954259251908 23.043510970214598 82-83 21.765933925851005 27.28447744413539 27.884122079816436 23.06546655019717 84-85 21.568999818190555 27.293920563901843 27.75775668045214 23.379322937455463 86-87 21.538402979457956 27.216264849775985 27.876749033929883 23.368583136836182 88-89 21.573083291999833 27.06537742001033 27.66051423903571 23.701025048954126 90-91 21.485731359557157 26.915350058710057 27.845393357376498 23.753525224356288 92-93 21.68426334784326 27.14158985043561 27.667753169649107 23.506393632072022 94-95 21.503459603822595 26.91494191501767 27.62756440129315 23.95403407986659 96-97 21.18010095701229 26.79723230248234 27.914047047096208 24.108619693409164 98-99 21.219055823857996 26.926030723706067 27.463687743326865 24.391225709109072 100-101 21.278597975146138 26.294000212895043 28.09470041553716 24.332701396421665 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1054.0 1 736.0 2 534.0 3 681.5 4 573.5 5 578.5 6 1030.5 7 1868.5 8 2933.5 9 3579.5 10 3413.5 11 3172.5 12 3451.5 13 3893.5 14 4253.0 15 4511.0 16 4767.0 17 5105.5 18 5508.0 19 6027.0 20 6860.0 21 8046.0 22 9711.0 23 11887.5 24 14226.0 25 17144.0 26 21583.0 27 27093.0 28 32015.5 29 37244.5 30 43779.5 31 51867.0 32 59571.5 33 66814.0 34 75723.0 35 84002.0 36 93803.5 37 103756.5 38 108708.0 39 111200.5 40 113658.5 41 118189.0 42 123208.0 43 127920.0 44 134596.5 45 139378.5 46 141162.5 47 153219.0 48 182917.5 49 182934.0 50 156800.0 51 145003.0 52 135526.5 53 129655.0 54 124155.5 55 113343.5 56 101725.5 57 89799.5 58 79232.5 59 69090.0 60 57680.0 61 45340.5 62 35019.0 63 26495.0 64 18784.0 65 13265.0 66 9107.5 67 6703.0 68 4773.0 69 2817.5 70 1694.5 71 1030.0 72 620.5 73 397.5 74 267.5 75 208.5 76 139.5 77 63.5 78 26.5 79 15.0 80 13.5 81 7.5 82 5.0 83 6.0 84 4.5 85 2.0 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4019399276523804 2 0.0 3 0.0 4 0.0 5 4.179527420834526E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0012930412958206817 16-17 0.002311801104649097 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0020636416640370473 36-37 0.007092135592228588 38-39 0.012447155100172823 40-41 0.002390167243789745 42-43 6.530511595053947E-4 44-45 3.9183069570323685E-4 46-47 2.6122046380215786E-4 48-49 2.742814869922658E-4 50-51 5.877460435548552E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.9591534785161842E-4 60-61 0.0021550688263678027 62-63 0.0 64-65 2.089763710417263E-4 66-67 0.0016587499451437024 68-69 0.0016979330147140262 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.12349197426247015 78-79 2.6122046380215788E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14653161916982047 88-89 0.4364340898974553 90-91 0.3349891227798873 92-93 0.0 94-95 0.0 96-97 5.224409276043157E-4 98-99 0.0 100-101 1.6979330147140264E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3828184.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.77227934770031 #Duplication Level Percentage of deduplicated Percentage of total 1 68.64205074968397 29.35976969664504 2 15.484498709895389 13.246146087575012 3 6.061732280817908 7.7782231933834804 4 3.051078165604858 5.220062704436799 5 1.7052835278251854 3.646943170958535 6 1.1210541682060902 2.8770025227848817 7 0.7461641340307106 2.234059854599751 8 0.5369768570164704 1.8374179305246878 9 0.39096101166520664 1.5050064244503363 >10 2.004868705366987 15.388516465578297 >50 0.1540991604206247 4.563087406667204 >100 0.09365084795687714 7.432822251739381 >500 0.004952550029256872 1.465013404235847 >1k 0.0022011333325629395 1.8308025471313476 >5k 2.445703702847711E-4 0.6266038486596448 >10k+ 1.834277777135783E-4 0.9885224906296292 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14932 0.3900543965493821 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12550 0.32783168207170815 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10317 0.2695011525046863 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6625 0.1730585572689296 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6395 0.16705048660147997 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5651 0.1476156840945994 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5289 0.13815950330496132 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4146 0.10830200429237466 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.6122046380215788E-5 0.0 4 0.0 0.0 0.0 2.6122046380215788E-5 0.0 5 0.0 0.0 0.0 2.6122046380215788E-5 0.0 6 0.0 0.0 0.0 2.6122046380215788E-5 0.0 7 0.0 0.0 0.0 5.2244092760431575E-5 0.0 8 0.0 0.0 0.0 5.2244092760431575E-5 0.0 9 0.0 0.0 0.0 1.5673227828129474E-4 0.0 10-11 1.3061023190107894E-5 0.0 0.0 2.4815944061205E-4 0.0 12-13 2.6122046380215788E-5 0.0 0.0 3.3958660294280523E-4 1.3061023190107894E-5 14-15 2.6122046380215788E-5 0.0 0.0 3.3958660294280523E-4 2.6122046380215788E-5 16-17 2.6122046380215788E-5 0.0 0.0 4.963188812240999E-4 2.6122046380215788E-5 18-19 2.6122046380215788E-5 0.0 0.0 6.530511595053948E-4 2.6122046380215788E-5 20-21 2.6122046380215788E-5 0.0 0.0 8.097834377866894E-4 2.6122046380215788E-5 22-23 5.2244092760431575E-5 0.0 0.0 0.001123247994349279 2.6122046380215788E-5 24-25 5.2244092760431575E-5 0.0 0.0 0.0014628345972920842 2.6122046380215788E-5 26-27 5.2244092760431575E-5 0.0 0.0 0.0022987400814589894 2.6122046380215788E-5 28-29 7.836613914064737E-5 0.0 0.0 0.006360718293582545 2.6122046380215788E-5 30-31 1.3061023190107896E-4 0.0 0.0 0.018220127350200514 2.6122046380215788E-5 32-33 1.3061023190107896E-4 0.0 0.0 0.04253975253018141 2.6122046380215788E-5 34-35 1.3061023190107896E-4 0.0 0.0 0.08193179847154683 2.6122046380215788E-5 36-37 1.3061023190107896E-4 0.0 0.0 0.14371043816075718 2.6122046380215788E-5 38-39 1.3061023190107896E-4 0.0 0.0 0.2374624626193516 2.6122046380215788E-5 40-41 1.4367125509118684E-4 0.0 0.0 0.3405400576356832 2.6122046380215788E-5 42-43 1.6979330147140264E-4 0.0 0.0 0.44545925692181987 2.6122046380215788E-5 44-45 1.8285432466151052E-4 0.0 0.0 0.5728825991645125 2.6122046380215788E-5 46-47 1.8285432466151052E-4 0.0 0.0 0.7296279384689973 2.6122046380215788E-5 48-49 1.8285432466151052E-4 0.0 0.0 0.8949674310325731 2.6122046380215788E-5 50-51 1.8285432466151052E-4 0.0 0.0 1.073890387713861 2.6122046380215788E-5 52-53 1.8285432466151052E-4 0.0 0.0 1.2510370452412947 2.6122046380215788E-5 54-55 1.8285432466151052E-4 0.0 0.0 1.4514845681398802 2.6122046380215788E-5 56-57 2.089763710417263E-4 0.0 0.0 1.6898482413593494 2.6122046380215788E-5 58-59 2.089763710417263E-4 0.0 0.0 1.9501805555845801 2.6122046380215788E-5 60-61 2.089763710417263E-4 0.0 0.0 2.2437662348518255 2.6122046380215788E-5 62-63 2.089763710417263E-4 0.0 0.0 2.542902848974866 2.6122046380215788E-5 64-65 2.089763710417263E-4 0.0 0.0 2.84958873450179 2.6122046380215788E-5 66-67 2.089763710417263E-4 0.0 0.0 3.2006951598982702 2.6122046380215788E-5 68-69 2.089763710417263E-4 0.0 0.0 3.601211436022929 2.6122046380215788E-5 70-71 2.089763710417263E-4 0.0 0.0 4.041616076970177 2.6122046380215788E-5 72-73 2.089763710417263E-4 0.0 0.0 4.487310432309419 2.6122046380215788E-5 74-75 2.089763710417263E-4 0.0 0.0 4.954803635352951 2.6122046380215788E-5 76-77 2.089763710417263E-4 0.0 0.0 5.453630233029552 2.6122046380215788E-5 78-79 2.089763710417263E-4 0.0 0.0 6.025258973967814 2.6122046380215788E-5 80-81 2.089763710417263E-4 0.0 0.0 6.641882939795997 2.6122046380215788E-5 82-83 2.089763710417263E-4 0.0 0.0 7.272416895321646 2.6122046380215788E-5 84-85 2.089763710417263E-4 0.0 0.0 7.922646873817977 2.6122046380215788E-5 86-87 2.350984174219421E-4 0.0 0.0 8.602081300167391 2.6122046380215788E-5 88-89 2.612204638021579E-4 0.0 0.0 9.333746758254044 2.6122046380215788E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 15525 0.0 27.314455 1 TACATGG 16170 0.0 25.619839 2 ACATGGG 16455 0.0 24.109076 3 GAGTACT 12505 0.0 21.745035 12-13 CATGGGA 9700 0.0 20.693825 4 TTTGCGT 730 0.0 20.151669 3 GACCGTA 165 6.375055E-5 20.13197 7 GTACTTT 13625 0.0 19.626667 14-15 CATGGGG 6830 0.0 19.523506 4 AGTACTT 13535 0.0 19.126068 12-13 ACCGTAC 180 1.2434881E-4 18.454306 8 TAGGACG 340 9.0403773E-10 18.144148 4 CTTTGCG 850 0.0 17.865007 2 GTATAGG 1020 0.0 17.750786 1 AGTACAT 11920 0.0 17.47674 2 GTATCAA 36175 0.0 17.056715 1 AGAGTAC 24245 0.0 16.979952 10-11 CCGTCCG 170 0.0018000801 16.748663 9 TACTTTT 16085 0.0 15.990698 14-15 ACTTTTT 16875 0.0 15.958788 16-17 >>END_MODULE