FastQCFastQC Report
Fri 27 May 2016
SRR939049_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939049_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3583156
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175980.4911312820318177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142630.39805690849072717No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92550.25829185221073264No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA38650.1078658032192849No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152100.032.141711
GTATCAA214650.028.2592661
GTACATG160350.027.6464161
TACATGG166200.026.5589222
ACATGGG164800.025.6062563
CATGGGA104150.023.6921254
ATCAACG257850.023.2353363
TCAACGC258350.023.2270344
TATCAAC261150.023.0142692
CAACGCA261200.022.991735
AACGCAG266250.022.5271576
GAGTACT135400.022.41149512-13
GTATAGG9650.020.1658951
ACGCAGA294850.020.1172837
CGCAGAG293700.020.0026328
GTACTTT151800.019.85072314-15
CATGGGG62950.019.7872584
AGTACTT142600.019.76941112-13
TATCACG2451.813496E-719.3312322
GCAGAGT301750.018.8885489
Produced by FastQC (version 0.11.5)