FastQCFastQC Report
Fri 27 May 2016
SRR939049_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939049_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3583156
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172340.48097263976226545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148120.4133785969687058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97620.2724413896576091No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA40240.1123032321227432No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154650.033.3078651
GTATCAA220500.028.4608731
GTACATG164400.025.5065611
TACATGG168600.024.262152
TCAACGC267550.023.2351744
ACATGGG167150.023.2234363
ATCAACG267750.023.1823733
TATCAAC269950.023.02862
CAACGCA271850.022.8851055
AACGCAG275950.022.579486
GAGTACT150500.021.90175812-13
CATGGGA108850.020.7519134
GTACTTT161700.020.28216414-15
AGTACTT153400.020.23464812-13
ACGCAGA305350.020.1412627
CGCAGAG306400.019.8089478
GCAGAGT319050.018.5783679
CATGGGG61500.018.36464
AGTACAT124500.017.8384082
TATAGGG6600.017.256272
Produced by FastQC (version 0.11.5)