##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939049_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3583156 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16462665873325 33.0 31.0 34.0 30.0 34.0 2 32.398795363640325 34.0 31.0 34.0 31.0 34.0 3 32.48473301190347 34.0 31.0 34.0 30.0 34.0 4 36.046184704210475 37.0 35.0 37.0 35.0 37.0 5 35.97196047283456 37.0 35.0 37.0 35.0 37.0 6 36.014442854288234 37.0 35.0 37.0 35.0 37.0 7 35.94722501615894 37.0 35.0 37.0 35.0 37.0 8 35.920397548976375 37.0 35.0 37.0 35.0 37.0 9 37.59154890269919 39.0 37.0 39.0 35.0 39.0 10-11 37.505089507685405 39.0 37.0 39.0 34.5 39.0 12-13 37.41252404305032 39.0 37.0 39.0 34.0 39.0 14-15 38.721247414290644 40.0 38.0 41.0 34.0 41.0 16-17 38.80356451128558 40.0 38.0 41.0 34.5 41.0 18-19 38.853909933031105 40.0 38.0 41.0 35.0 41.0 20-21 38.83205168851147 40.0 38.0 41.0 34.0 41.0 22-23 38.7537987461333 40.0 38.0 41.0 34.0 41.0 24-25 38.677850615490925 40.0 38.0 41.0 34.0 41.0 26-27 38.515268802139786 40.0 38.0 41.0 34.0 41.0 28-29 38.3515445601587 40.0 38.0 41.0 34.0 41.0 30-31 38.13923061122652 40.0 37.5 41.0 33.0 41.0 32-33 38.02688733061022 40.0 37.0 41.0 33.0 41.0 34-35 37.922258617821825 40.0 37.0 41.0 33.0 41.0 36-37 37.782811995905284 40.0 37.0 41.0 33.0 41.0 38-39 37.63167400470423 40.0 37.0 41.0 33.0 41.0 40-41 37.44560005202118 40.0 37.0 41.0 32.0 41.0 42-43 37.250655846410254 40.0 36.5 41.0 31.5 41.0 44-45 36.9795126140196 40.0 36.0 41.0 31.0 41.0 46-47 36.62922169729702 40.0 36.0 41.0 30.5 41.0 48-49 36.269789956116895 39.0 35.5 41.0 30.0 41.0 50-51 35.99659224996065 39.0 35.0 41.0 29.0 41.0 52-53 35.72592443644653 39.0 35.0 40.0 28.0 41.0 54-55 35.41812818085509 38.5 35.0 40.0 28.0 41.0 56-57 35.28920928924111 38.0 35.0 40.0 27.5 41.0 58-59 35.01453927766472 38.0 34.0 40.0 27.0 41.0 60-61 34.657718781989956 38.0 34.0 40.0 26.0 41.0 62-63 34.236116150120175 37.0 34.0 40.0 26.0 41.0 64-65 33.81762097435892 36.5 33.0 39.5 25.0 41.0 66-67 33.36961926860008 36.0 33.0 39.0 23.5 40.0 68-69 32.882548652640295 35.5 32.0 39.0 22.5 40.0 70-71 32.364843450857286 35.0 31.5 38.0 21.5 40.0 72-73 31.859390715894033 35.0 31.0 37.0 20.0 39.5 74-75 31.32288030440204 35.0 31.0 37.0 18.5 39.0 76-77 29.41672076236703 32.5 28.5 35.0 13.5 37.0 78-79 30.391075493224406 34.0 30.0 36.0 15.5 38.0 80-81 30.377428445761222 34.0 30.0 35.5 13.0 37.0 82-83 29.965771515390344 34.0 30.0 35.0 7.5 37.0 84-85 29.51879809307772 34.0 29.5 35.0 4.5 36.0 86-87 28.811030834270124 34.0 29.0 35.0 2.0 36.0 88-89 28.12024190406446 33.0 27.5 35.0 2.0 35.0 90-91 27.533161966713145 33.0 26.5 35.0 2.0 35.0 92-93 27.11770308074781 33.0 26.0 34.5 2.0 35.0 94-95 26.559733653795703 32.0 25.0 34.0 2.0 35.0 96-97 25.703423741528418 32.0 23.5 34.0 2.0 35.0 98-99 24.475194353804298 32.0 10.5 34.0 2.0 35.0 100-101 18.881257053837455 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15718666999999442 1101 2 0.2117107774473581 1101 3 0.2417178785367824 1101 4 0.17308268147251482 1101 5 0.10961161749284543 1101 6 0.15264747488714647 1101 7 0.21566105448117412 1101 8 0.17781808928425136 1101 9 0.25415093445370474 1101 10-11 0.3465729795614223 1101 12-13 0.34184656553753 1101 14-15 0.4167936870906317 1101 16-17 0.3941575929426264 1101 18-19 0.37001065301883074 1101 20-21 0.38589830928441415 1101 22-23 0.43000469020619647 1101 24-25 0.29547051011549286 1101 26-27 0.3115305343104353 1101 28-29 0.317478903976415 1101 30-31 0.2946813458778266 1101 32-33 0.4196943799309807 1101 34-35 0.534951486990046 1101 36-37 0.6181906164960012 1101 38-39 0.5554718385865698 1101 40-41 0.4890332153736239 1101 42-43 0.5253036802659565 1101 44-45 0.6248606026265406 1101 46-47 0.5709839300052622 1101 48-49 0.515130617094961 1101 50-51 0.4379491485188254 1101 52-53 0.45768675237913214 1101 54-55 0.5627572157345355 1101 56-57 0.4615655912790615 1101 58-59 0.5748580280206212 1101 60-61 0.639606060703052 1101 62-63 0.6882857517631891 1101 64-65 0.5111624959103906 1101 66-67 0.5236663836512108 1101 68-69 0.6041431562474955 1101 70-71 0.6404016352311643 1101 72-73 0.642759250329842 1101 74-75 0.663512950470885 1101 76-77 0.4027286644792376 1101 78-79 0.7495403165853709 1101 80-81 0.8801225538822557 1101 82-83 0.7493607532828257 1101 84-85 0.7016096285782716 1101 86-87 1.029351344316737 1101 88-89 1.038775291899956 1101 90-91 1.1066300039764094 1101 92-93 1.1062699358294203 1101 94-95 1.138148336525262 1101 96-97 1.182253781446608 1101 98-99 1.468000965362915 1101 100-101 1.6247796303929292 1102 1 0.1403648577263823 1102 2 0.14617266341214474 1102 3 0.14644512115682318 1102 4 0.08475919292892797 1102 5 0.11682633342482518 1102 6 0.11597885365218019 1102 7 0.1786988284326938 1102 8 0.13183094169743725 1102 9 0.21398814554922296 1102 10-11 0.17028389452781312 1102 12-13 0.16187362118846949 1102 14-15 0.3237705635438033 1102 16-17 0.30911578385959615 1102 18-19 0.22105949537765923 1102 20-21 0.26818543577159204 1102 22-23 0.2544910146459429 1102 24-25 0.29174418112948075 1102 26-27 0.26651310309801346 1102 28-29 0.23277927643216145 1102 30-31 0.2169050671514725 1102 32-33 0.14174148145288257 1102 34-35 0.2408803462360467 1102 36-37 0.19699670315035434 1102 38-39 0.32956321373772113 1102 40-41 0.279912324500728 1102 42-43 0.3548354575093171 1102 44-45 0.31035231982869504 1102 46-47 0.38698680955149456 1102 48-49 0.3769593900123809 1102 50-51 0.3169269040149132 1102 52-53 0.25488521795133323 1102 54-55 0.2828490807553905 1102 56-57 0.3772153245592733 1102 58-59 0.3767840998390213 1102 60-61 0.412027692506193 1102 62-63 0.5887002949074827 1102 64-65 0.5819084824332847 1102 66-67 0.36616185300913173 1102 68-69 0.26619529818738386 1102 70-71 0.37428145827762194 1102 72-73 0.5677767393494939 1102 74-75 0.452600408249463 1102 76-77 0.17991351924461796 1102 78-79 0.394889151084282 1102 80-81 0.5158019627864938 1102 82-83 0.5067534828668236 1102 84-85 0.6067967184324381 1102 86-87 0.7633070160503301 1102 88-89 0.5364984793424341 1102 90-91 0.504176282630965 1102 92-93 0.47249014727150396 1102 94-95 0.6992853619569956 1102 96-97 0.7378951103006877 1102 98-99 1.2303364466701545 1102 100-101 1.3262459575384327 1103 1 0.061159980532274005 1103 2 0.0873533233258712 1103 3 0.14113938697567363 1103 4 0.1039022691934548 1103 5 0.16101068385501804 1103 6 0.11810758957627598 1103 7 0.17990647132639737 1103 8 0.18704001417518867 1103 9 0.21766460450044178 1103 10-11 0.17590866911883296 1103 12-13 0.175271363384077 1103 14-15 0.20186445023799848 1103 16-17 0.22402001680992356 1103 18-19 0.2603286033101426 1103 20-21 0.24105424676467635 1103 22-23 0.26019841204517746 1103 24-25 0.27713181383101926 1103 26-27 0.21774986645865368 1103 28-29 0.18649323450129174 1103 30-31 0.29761735675191403 1103 32-33 0.3669685103106346 1103 34-35 0.31725598573658687 1103 36-37 0.23608734581709 1103 38-39 0.21264556302952542 1103 40-41 0.34275797876443903 1103 42-43 0.25267275081032636 1103 44-45 0.28365858949874223 1103 46-47 0.2596495615882901 1103 48-49 0.2800901753545091 1103 50-51 0.3121224459023111 1103 52-53 0.30843066273016007 1103 54-55 0.3543927522392849 1103 56-57 0.5027828617926033 1103 58-59 0.4594103991583225 1103 60-61 0.4029947619873795 1103 62-63 0.3870573066187859 1103 64-65 0.4171653057738993 1103 66-67 0.33346239875722006 1103 68-69 0.35512844083503126 1103 70-71 0.3334543867446129 1103 72-73 0.32521907607705103 1103 74-75 0.3996511044874289 1103 76-77 0.2000557030897241 1103 78-79 0.35535355550277004 1103 80-81 0.2833716680423066 1103 82-83 0.3344532285095987 1103 84-85 0.5701918353174129 1103 86-87 0.6352996622014686 1103 88-89 0.6309151232160666 1103 90-91 0.7294271440959292 1103 92-93 0.8248881544186766 1103 94-95 0.8799900549138826 1103 96-97 0.8091815571996825 1103 98-99 0.9686989193999729 1103 100-101 1.098721083651096 1104 1 -0.024388073282608502 1104 2 0.006867372244407477 1104 3 0.03614484482891811 1104 4 0.0074605199851802695 1104 5 -0.05304569150154492 1104 6 -0.014749765049423047 1104 7 0.020678746330325737 1104 8 -0.001029882282821859 1104 9 0.036656586172838956 1104 10-11 0.0307641819998139 1104 12-13 0.03328766956290963 1104 14-15 0.13154313316592692 1104 16-17 0.014671635074641642 1104 18-19 0.06610103553617819 1104 20-21 0.06793985644955569 1104 22-23 0.06845028186526747 1104 24-25 0.02030347791475151 1104 26-27 -0.12856835109182185 1104 28-29 -0.0737057188486574 1104 30-31 -0.07035551120397798 1104 32-33 0.04674781538890471 1104 34-35 0.05248034168744198 1104 36-37 0.08657698038798856 1104 38-39 0.028149370050620348 1104 40-41 0.1075167775875201 1104 42-43 0.06297964836889491 1104 44-45 0.17749422501297119 1104 46-47 0.19083452217046926 1104 48-49 0.017418208056703577 1104 50-51 0.039989435997313194 1104 52-53 -0.10718553563922484 1104 54-55 0.07237190907956403 1104 56-57 0.054106851640547404 1104 58-59 0.006935363783348691 1104 60-61 0.034052687104725976 1104 62-63 0.011781112292261753 1104 64-65 -0.012347081082339173 1104 66-67 0.01830313275986839 1104 68-69 0.0875183516676401 1104 70-71 0.10821875572244011 1104 72-73 0.004075128005265327 1104 74-75 -0.012797697255489737 1104 76-77 -0.12183814952577876 1104 78-79 0.08913789168856567 1104 80-81 0.018793809407444684 1104 82-83 0.07620926471560452 1104 84-85 0.30258134186459174 1104 86-87 0.2504914507152698 1104 88-89 0.20265247245129459 1104 90-91 0.34332733051643416 1104 92-93 0.38255062100897774 1104 94-95 0.6805013412153436 1104 96-97 0.42529614965751605 1104 98-99 0.5762233000571619 1104 100-101 0.8593770142936812 1105 1 0.060768656749665695 1105 2 0.07868472384257785 1105 3 0.0667435041119262 1105 4 0.11072966730213096 1105 5 0.044496413704798954 1105 6 0.034843291720548564 1105 7 0.1316173993523364 1105 8 0.13317334105126832 1105 9 0.1411923548181946 1105 10-11 0.18813965832136859 1105 12-13 0.20058324260607918 1105 14-15 0.13230205238826898 1105 16-17 0.07421039388592021 1105 18-19 0.08219758443132719 1105 20-21 0.18318719822444507 1105 22-23 0.1322248859651367 1105 24-25 0.05866603283341476 1105 26-27 0.12692938739279924 1105 28-29 0.14133656447226173 1105 30-31 0.14342800941953726 1105 32-33 0.2263216789639344 1105 34-35 0.2527044853881293 1105 36-37 0.23548184561462193 1105 38-39 0.22058604222264933 1105 40-41 0.27811098940353673 1105 42-43 0.2915013405653184 1105 44-45 0.2940109947701899 1105 46-47 0.22885375854469459 1105 48-49 0.23812414628872602 1105 50-51 0.27900119155934533 1105 52-53 0.2410135640725528 1105 54-55 0.3146856282198698 1105 56-57 0.2558803114892001 1105 58-59 0.1283126104558434 1105 60-61 0.14444542434794272 1105 62-63 0.23691327330783452 1105 64-65 0.20228011971216375 1105 66-67 0.3038238642170654 1105 68-69 0.3255320610925949 1105 70-71 0.5051314795800792 1105 72-73 0.3829717675769295 1105 74-75 0.29292951757227925 1105 76-77 0.1252976601399034 1105 78-79 0.19594920898079238 1105 80-81 0.21188296384897498 1105 82-83 0.2631542650934229 1105 84-85 0.27998959126817624 1105 86-87 0.4503727508437301 1105 88-89 0.6823222804113946 1105 90-91 0.44530243765504807 1105 92-93 0.7908166054428456 1105 94-95 0.821533369454599 1105 96-97 0.5916024851390169 1105 98-99 0.9876836020963005 1105 100-101 1.0244666005281253 1106 1 0.08123114501402995 1106 2 0.059036343058537 1106 3 0.04939599690264629 1106 4 0.010519147737362289 1106 5 0.024532564173490812 1106 6 0.03724741877034177 1106 7 0.11949560927622116 1106 8 0.06434398697814459 1106 9 0.06489903368947836 1106 10-11 0.09160118490132874 1106 12-13 0.09692502235348144 1106 14-15 0.0035013171563633705 1106 16-17 -0.05151389146572427 1106 18-19 -0.037847675848013296 1106 20-21 0.061789233476361005 1106 22-23 -0.004199103695405881 1106 24-25 0.08296459755974439 1106 26-27 0.08585847089145204 1106 28-29 0.12149145643722647 1106 30-31 0.08631444100630148 1106 32-33 0.1433236815776766 1106 34-35 0.043862915505506805 1106 36-37 0.038288023228062684 1106 38-39 -0.019081684736889315 1106 40-41 0.21527654887955094 1106 42-43 0.14941150534106384 1106 44-45 0.22679730574347445 1106 46-47 0.2884808355345214 1106 48-49 0.3417294386421261 1106 50-51 0.12770794598196034 1106 52-53 0.08596218581494952 1106 54-55 0.16495801592374448 1106 56-57 0.25792422661921677 1106 58-59 0.2303255327541649 1106 60-61 0.25509642323717685 1106 62-63 0.32919573082784837 1106 64-65 0.2909154210511318 1106 66-67 0.34435181854242813 1106 68-69 0.3338467780202592 1106 70-71 0.3732154275094288 1106 72-73 0.3628455666466728 1106 74-75 0.4403718282608047 1106 76-77 0.2368092305732148 1106 78-79 0.44991468807019785 1106 80-81 0.3957555349578783 1106 82-83 0.36071801194209385 1106 84-85 0.39798388496068426 1106 86-87 0.6857304846801107 1106 88-89 0.7648128667165288 1106 90-91 0.9727480507758592 1106 92-93 0.9715934610840797 1106 94-95 0.9829538501759174 1106 96-97 0.8282386313096737 1106 98-99 0.9162746740117598 1106 100-101 1.1713548262069793 1107 1 -0.004694599757932849 1107 2 0.05640644584485699 1107 3 0.01818974394798545 1107 4 0.02181914459444556 1107 5 0.07097514775203706 1107 6 0.05283307098353873 1107 7 0.07115369186409737 1107 8 0.085891023277469 1107 9 -0.0333746727075237 1107 10-11 0.12260012023984501 1107 12-13 0.07211119897467455 1107 14-15 0.06887798208133233 1107 16-17 0.1309179171623569 1107 18-19 0.0740006282234944 1107 20-21 0.05439888837150875 1107 22-23 0.13797457854045803 1107 24-25 0.02532498023548868 1107 26-27 0.10327630174516145 1107 28-29 0.07457428494029728 1107 30-31 0.19217210080701363 1107 32-33 0.17970452657579727 1107 34-35 0.21080221343131456 1107 36-37 0.2425493210150549 1107 38-39 0.10077568434445539 1107 40-41 0.09028612493999333 1107 42-43 0.21300370823438186 1107 44-45 0.22575642071250712 1107 46-47 0.15996097855011726 1107 48-49 0.16881500638956481 1107 50-51 0.09028326947273513 1107 52-53 0.14215802787313692 1107 54-55 0.17773100730823188 1107 56-57 0.22307227742438585 1107 58-59 0.29616184858016226 1107 60-61 0.3218022371663025 1107 62-63 0.3186341955545515 1107 64-65 0.26364230984311376 1107 66-67 0.35269964692108857 1107 68-69 0.27569074912903346 1107 70-71 0.5363653882885089 1107 72-73 0.47532908272725294 1107 74-75 0.3485712522566793 1107 76-77 0.4255825192248146 1107 78-79 0.5564562586488755 1107 80-81 0.5392257502997424 1107 82-83 0.36922470470802793 1107 84-85 0.5406269987746981 1107 86-87 0.5976586383632743 1107 88-89 0.7272515062677165 1107 90-91 0.8457946981398088 1107 92-93 0.9058807337303953 1107 94-95 0.9711827848072758 1107 96-97 1.2082193285909248 1107 98-99 1.0281156937296068 1107 100-101 1.1890202841620159 1108 1 0.014907193598702406 1108 2 0.010430549851342619 1108 3 0.09594614583424033 1108 4 0.10549113960539103 1108 5 0.07778173912245734 1108 6 0.07599364778979378 1108 7 0.11620766851093833 1108 8 0.16113197679683822 1108 9 0.12727094979725706 1108 10-11 0.15838671523678016 1108 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-1.1068386148371658 2316 52-53 -1.093880783942197 2316 54-55 -1.0521875083994559 2316 56-57 -1.0382476287010718 2316 58-59 -1.18303370680308 2316 60-61 -1.0567215009556392 2316 62-63 -0.8201409006715537 2316 64-65 -0.9118921567045888 2316 66-67 -0.8953781479696445 2316 68-69 -0.8430692391386074 2316 70-71 -0.9478417761001481 2316 72-73 -0.8430051897953099 2316 74-75 -1.1005920796029294 2316 76-77 -0.9776005816869251 2316 78-79 -1.0636494983657094 2316 80-81 -1.1676988155096204 2316 82-83 -1.0512034507099735 2316 84-85 -1.4359535770690428 2316 86-87 -1.2806843433608428 2316 88-89 -1.5656725415843304 2316 90-91 -1.8650947930310942 2316 92-93 -1.7386285075913719 2316 94-95 -1.6388052478725932 2316 96-97 -1.3977011793205207 2316 98-99 -1.546823774578339 2316 100-101 -0.8277005474205339 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 8.0 4 29.0 5 87.0 6 202.0 7 566.0 8 1137.0 9 2118.0 10 3618.0 11 5779.0 12 8650.0 13 11907.0 14 14350.0 15 18928.0 16 24798.0 17 35214.0 18 34764.0 19 23468.0 20 21171.0 21 22836.0 22 24654.0 23 26935.0 24 31307.0 25 37049.0 26 44197.0 27 53861.0 28 64948.0 29 78829.0 30 94908.0 31 114284.0 32 142123.0 33 181034.0 34 242882.0 35 348159.0 36 542203.0 37 802766.0 38 499680.0 39 23704.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.81163578491394 20.05474938287863 14.234055012762713 22.89955981944472 2 15.687762408334999 21.48580748368198 39.321536656511746 23.504893451471272 3 18.802474689910238 25.630086995933194 30.8539455161874 24.713492797969163 4 12.845938050143504 17.189846046334573 37.144210299523664 32.82000560399826 5 13.025639966554625 38.22577080093638 34.63851420367966 14.110075028829334 6 31.100599583160765 36.784332024617406 17.47548808927102 14.639580302950804 7 27.50625426300167 33.83916301718373 21.32008765457044 17.33449506524416 8 25.92253867819319 35.51263188094518 20.1579278155905 18.40690162527113 9 26.510511962080358 16.407239874568678 20.929286919129396 36.15296124422158 10-11 24.575751097635713 26.986349463992077 27.57987651109804 20.85802292727417 12-13 25.507039046025348 24.631079417139528 28.2625009907467 21.59938054608842 14-15 22.49790999155903 25.515811013280466 26.866659066213117 25.11961992894739 16-17 21.44990097147451 29.00395011691827 27.606540613277737 21.939608298329485 18-19 21.36013777871178 27.33322783918114 30.1629968376872 21.143637544419875 20-21 22.09638653745469 26.63273661543064 30.1197324369913 21.151144410123365 22-23 21.924889678261287 26.668459313521375 29.852537818615765 21.55411318960157 24-25 21.715277259340763 26.799222193306033 30.07029424508167 21.41520630227153 26-27 21.64705219118469 26.894316262799826 30.037065121137722 21.421566424877756 28-29 21.370350607118418 26.84546807339675 30.359828039862062 21.424353279622768 30-31 21.485905162934575 26.934272468181682 30.12951431642943 21.450308052454318 32-33 21.35001936840037 27.082340260931982 29.934281398856204 21.633358971811443 34-35 21.7711244484731 26.870523150697075 29.906236065724773 21.45211633510506 36-37 21.84443145471731 26.711447727421184 29.757185152183585 21.68693566567792 38-39 21.702151829820824 26.955531120512045 29.942308145870978 21.40000890379616 40-41 21.647442637015693 26.875595357876197 30.031197938893012 21.4457640662151 42-43 21.541277428764964 27.043176276171426 29.641682038022722 21.77386425704089 44-45 21.65791605412379 27.223333540324568 29.34446806625301 21.774282339298633 46-47 21.762145421455863 27.291909443459655 28.93477020448301 22.01117493060148 48-49 21.915503791267547 27.424378735745503 28.748325617112748 21.9117918558742 50-51 21.85213460687519 27.62454507308623 28.54561116319933 21.97770915683925 52-53 21.958658791300184 27.708045644677487 28.471227599356546 21.862067964665783 54-55 21.590324283955262 27.59563077912321 28.576777008871506 22.23726792805002 56-57 21.691003685019574 27.96859807387677 28.33885825791565 22.001539983188007 58-59 21.79804729166655 27.45689099406496 28.587942483741852 22.15711923052664 60-61 21.83476854507067 27.491261046664967 28.652296408551827 22.021673999712537 62-63 21.818615671860478 27.429914236515206 28.74636837830823 22.005101713316087 64-65 21.622550901383832 27.397321252274025 28.738453354811767 22.24167449153038 66-67 21.420083068708347 27.68145254695849 28.441585372048124 22.456879012285036 68-69 21.420218601653843 27.537567885657094 28.58978684901426 22.4524266636748 70-71 21.370820041390903 27.5443048855415 28.473779179770226 22.611095893297374 72-73 21.501171034696785 27.43009235433791 28.280292568897362 22.788444042067944 74-75 21.3680342134141 27.727804762058923 28.080943168536344 22.82321785599064 76-77 21.398446294935795 27.389624646980444 28.44312439417514 22.76880466390862 78-79 21.24708696271033 27.42428893980793 28.325090775126082 23.00353332235566 80-81 21.119237273507313 27.721913609145933 28.253904286052578 22.90494483129418 82-83 21.162684516108147 27.55333566275094 28.239155649377253 23.044824171763665 84-85 21.07883023022065 27.311084843096832 28.109488593763672 23.500596332918846 86-87 21.066539248429805 27.118309564859832 28.414215606853098 23.400935579857265 88-89 21.154490870255028 26.947281819557894 28.31165591204947 23.58657139813761 90-91 20.836791233250082 27.04604942055777 28.512432469386027 23.60472687680612 92-93 20.824839889750823 27.125054002672506 28.291707087271668 23.758399020305003 94-95 20.67542802425986 26.816792792762623 28.371471179939384 24.136308003038135 96-97 20.718277607304177 26.641186328807994 28.253061896568806 24.387474167319027 98-99 20.493874673611753 26.633643637061855 28.27839480056129 24.594086888765098 100-101 20.512347141624783 25.999061716032628 29.0772722337996 24.411318908542988 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1892.0 1 1364.5 2 1026.5 3 1257.5 4 1161.0 5 1189.0 6 1974.5 7 3643.5 8 5489.0 9 6331.5 10 5770.5 11 5154.0 12 5316.0 13 5680.5 14 6041.0 15 6330.5 16 6636.0 17 6941.5 18 7301.0 19 7894.5 20 8685.0 21 10030.0 22 11939.0 23 14207.0 24 16566.0 25 19161.0 26 23560.5 27 28999.5 28 32908.0 29 37200.0 30 43249.0 31 49548.5 32 56147.0 33 63819.0 34 73579.5 35 80866.0 36 88453.0 37 98165.0 38 102400.5 39 103069.0 40 104623.5 41 108638.0 42 112702.0 43 117524.0 44 123269.0 45 127021.0 46 128598.5 47 131460.5 48 145787.5 49 148708.0 50 137436.0 51 132386.0 52 125755.5 53 120845.0 54 115040.0 55 104806.5 56 94485.5 57 83648.5 58 74135.5 59 65494.0 60 55951.5 61 45209.0 62 35976.5 63 28148.0 64 20629.5 65 14996.5 66 10126.0 67 7061.0 68 4771.0 69 2741.5 70 1800.0 71 1164.5 72 780.0 73 539.0 74 391.0 75 244.5 76 123.0 77 61.5 78 29.5 79 16.5 80 9.0 81 6.0 82 4.0 83 2.0 84 1.5 85 2.0 86 1.5 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3953497977760388 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0010186550627435701 16-17 0.0027210648936300845 18-19 5.302587997843242E-4 20-21 0.0 22-23 0.0 24-25 9.767925259184921E-5 26-27 8.372507365015645E-5 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01363323282603381 36-37 0.023387203906277035 38-39 0.011568014342663285 40-41 0.0063351972395285045 42-43 0.005386313071493398 44-45 0.0020512643044288334 46-47 0.001297738641577425 48-49 0.0033350487670645657 50-51 7.116631260263299E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 8.372507365015646E-4 60-61 0.0017024098308865145 62-63 0.0012837844626357323 64-65 7.395714839097153E-4 66-67 0.0027350190725717776 68-69 0.0030420110092890178 70-71 4.1862536825078226E-5 72-73 0.0 74-75 0.0 76-77 0.09282319832014012 78-79 0.0035164530933065714 80-81 2.930377577755476E-4 82-83 0.0 84-85 1.8140432624200566E-4 86-87 0.09332554876204106 88-89 0.43804958533761856 90-91 0.3916658945354319 92-93 0.0 94-95 9.907467048601848E-4 96-97 2.7908357883385485E-5 98-99 0.0 100-101 5.302587997843242E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3583156.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.848955582908324 #Duplication Level Percentage of deduplicated Percentage of total 1 65.72885951379503 26.192264032804395 2 15.600695553267663 12.43342848329277 3 6.800838509353353 8.130189350572628 4 3.4936605962548004 5.568749036876581 5 2.104757708462908 4.193619821866117 6 1.3640739532235593 3.261415342428467 7 0.920728139847754 2.5683038324108867 8 0.6526826517437547 2.0806977599257364 9 0.4992786922913075 1.790616098934798 >10 2.5558020290044796 17.999646433548953 >50 0.16993663475813864 4.6638235218493 >100 0.10182648802182619 7.433893101770065 >500 0.005039654678184566 1.3795002264804872 >1k 0.0016098896861502993 1.1377292685751654 >5k 6.999520374566518E-5 0.2722851953868783 >10k+ 1.3999040749133036E-4 0.8938384932767773 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17234 0.48097263976226545 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14812 0.4133785969687058 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9762 0.2724413896576091 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4024 0.1123032321227432 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.7908357883385485E-5 0.0 2 0.0 0.0 0.0 2.7908357883385485E-5 0.0 3 0.0 0.0 0.0 2.7908357883385485E-5 0.0 4 0.0 0.0 0.0 5.581671576677097E-5 0.0 5 0.0 0.0 0.0 5.581671576677097E-5 0.0 6 0.0 0.0 0.0 5.581671576677097E-5 0.0 7 0.0 0.0 0.0 5.581671576677097E-5 0.0 8 0.0 0.0 0.0 8.372507365015645E-5 0.0 9 0.0 0.0 0.0 8.372507365015645E-5 0.0 10-11 0.0 0.0 0.0 9.76792525918492E-5 0.0 12-13 0.0 0.0 0.0 1.1163343153354194E-4 0.0 14-15 0.0 0.0 0.0 1.1163343153354194E-4 1.3954178941692742E-5 16-17 0.0 0.0 0.0 1.8140432624200566E-4 2.7908357883385485E-5 18-19 0.0 0.0 0.0 2.651293998921621E-4 2.7908357883385485E-5 20-21 0.0 0.0 0.0 3.907170103673968E-4 2.7908357883385485E-5 22-23 0.0 0.0 0.0 5.023504419009387E-4 2.7908357883385485E-5 24-25 0.0 0.0 0.0 8.930674522683355E-4 2.7908357883385485E-5 26-27 0.0 0.0 0.0 0.0018279974413617493 2.7908357883385485E-5 28-29 0.0 0.0 0.0 0.005009550240067695 2.7908357883385485E-5 30-31 0.0 0.0 0.0 0.015935672351413112 2.7908357883385485E-5 32-33 0.0 0.0 0.0 0.03558315630131649 2.7908357883385485E-5 34-35 0.0 0.0 0.0 0.06822198084593581 2.7908357883385485E-5 36-37 0.0 0.0 0.0 0.12127576918225164 2.7908357883385485E-5 38-39 0.0 0.0 0.0 0.19971220901350653 2.7908357883385485E-5 40-41 0.0 0.0 0.0 0.28624207263094326 2.7908357883385485E-5 42-43 0.0 0.0 0.0 0.37660933545734543 2.7908357883385485E-5 44-45 0.0 0.0 0.0 0.48388906316107927 2.7908357883385485E-5 46-47 0.0 0.0 0.0 0.6185608441273559 2.7908357883385485E-5 48-49 0.0 0.0 0.0 0.7677031086561679 2.7908357883385485E-5 50-51 0.0 0.0 0.0 0.9296971719902789 2.7908357883385485E-5 52-53 0.0 0.0 0.0 1.0913981975666145 2.7908357883385485E-5 54-55 0.0 0.0 0.0 1.27189550217741 2.7908357883385485E-5 56-57 0.0 0.0 0.0 1.4862177365428688 2.7908357883385485E-5 58-59 0.0 0.0 0.0 1.7267877814976518 2.7908357883385485E-5 60-61 0.0 0.0 0.0 1.9952103676200534 2.7908357883385485E-5 62-63 0.0 0.0 0.0 2.267609894740837 2.7908357883385485E-5 64-65 0.0 0.0 0.0 2.5490796381737217 2.7908357883385485E-5 66-67 0.0 0.0 0.0 2.876821438977259 2.7908357883385485E-5 68-69 0.0 0.0 0.0 3.249411970899397 2.7908357883385485E-5 70-71 0.0 0.0 0.0 3.662790567868103 2.7908357883385485E-5 72-73 0.0 0.0 0.0 4.085281243685734 2.7908357883385485E-5 74-75 0.0 0.0 0.0 4.521098160392682 2.7908357883385485E-5 76-77 0.0 0.0 0.0 4.99368433861099 2.7908357883385485E-5 78-79 0.0 0.0 0.0 5.5288410552038485 2.7908357883385485E-5 80-81 0.0 0.0 0.0 6.1086930069469485 2.7908357883385485E-5 82-83 0.0 0.0 0.0 6.708904105765978 2.7908357883385485E-5 84-85 0.0 0.0 0.0 7.328483604956078 2.7908357883385485E-5 86-87 0.0 0.0 0.0 7.97990654049112 2.7908357883385485E-5 88-89 0.0 0.0 0.0 8.672564074798863 2.7908357883385485E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15465 0.0 33.307865 1 GTATCAA 22050 0.0 28.460873 1 GTACATG 16440 0.0 25.506561 1 TACATGG 16860 0.0 24.26215 2 TCAACGC 26755 0.0 23.235174 4 ACATGGG 16715 0.0 23.223436 3 ATCAACG 26775 0.0 23.182373 3 TATCAAC 26995 0.0 23.0286 2 CAACGCA 27185 0.0 22.885105 5 AACGCAG 27595 0.0 22.57948 6 GAGTACT 15050 0.0 21.901758 12-13 CATGGGA 10885 0.0 20.751913 4 GTACTTT 16170 0.0 20.282164 14-15 AGTACTT 15340 0.0 20.234648 12-13 ACGCAGA 30535 0.0 20.141262 7 CGCAGAG 30640 0.0 19.808947 8 GCAGAGT 31905 0.0 18.578367 9 CATGGGG 6150 0.0 18.3646 4 AGTACAT 12450 0.0 17.838408 2 TATAGGG 660 0.0 17.25627 2 >>END_MODULE