FastQCFastQC Report
Fri 27 May 2016
SRR939048_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939048_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3597000
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176400.49040867389491244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141350.3929663608562691No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92530.2572421462329719No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA40530.11267723102585488No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA36520.1015290519877676No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145500.030.5635931
GTATCAA211750.029.3971941
ACGCGTC1606.48688E-826.616218
GTACATG162500.026.1408181
TACATGG170100.024.3407212
ATCAACG255250.024.2110253
TCAACGC256900.024.0739424
TATCAAC257750.024.0676522
CAACGCA258150.023.9390435
AACGCAG263600.023.4783676
ACATGGG170300.023.228833
GAGTACT132450.022.096512-13
AGTACTT137100.021.19174212-13
ACGCAGA291150.021.0132547
CGCAGAG291950.020.745568
GTACTTT144650.020.47890714-15
CATGGGA108850.019.8231264
AGTACAT123000.019.658282
CATGGGG65500.019.5777914
GCAGAGT299650.019.3916079
Produced by FastQC (version 0.11.5)