FastQCFastQC Report
Fri 27 May 2016
SRR939048_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939048_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3597000
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171340.47634139560745065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149760.41634695579649705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99250.2759243814289686No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA40830.11351125938281902No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA155500.031.6873871
GTATCAA221000.028.2040671
GTACATG160800.025.486411
TACATGG166250.023.9732192
ATCAACG269000.023.053243
TATCAAC270800.022.987612
TCAACGC270400.022.9865234
ACATGGG165850.022.9439163
CAACGCA274600.022.6349455
AACGCAG279150.022.249016
GAGTACT148400.021.67907312-13
CATGGGG62650.020.2208254
AGTACTT153150.019.93776512-13
GTACTTT161850.019.93614214-15
ACGCAGA308250.019.8407697
CGCAGAG306800.019.5788428
GCAGAGT317200.018.5781789
GTATAGG9350.018.3479331
CATGGGA107850.018.213314
AGTACAT122450.018.095342
Produced by FastQC (version 0.11.5)