##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939048_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3597000 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14035752015568 33.0 31.0 34.0 30.0 34.0 2 32.36412065610231 34.0 31.0 34.0 30.0 34.0 3 32.455235195996664 34.0 31.0 34.0 30.0 34.0 4 36.0411679177092 37.0 35.0 37.0 35.0 37.0 5 35.958806227411735 37.0 35.0 37.0 35.0 37.0 6 36.0017364470392 37.0 35.0 37.0 35.0 37.0 7 35.9388448707256 37.0 35.0 37.0 35.0 37.0 8 35.90733861551293 37.0 35.0 37.0 35.0 37.0 9 37.581750347511814 39.0 37.0 39.0 35.0 39.0 10-11 37.50402877397832 39.0 37.0 39.0 34.5 39.0 12-13 37.42791548512649 39.0 37.0 39.0 34.0 39.0 14-15 38.68318348623853 40.0 38.0 41.0 34.0 41.0 16-17 38.67297484014456 40.0 38.0 41.0 34.0 41.0 18-19 38.779602307478456 40.0 38.0 41.0 34.0 41.0 20-21 38.78474159021407 40.0 38.0 41.0 34.0 41.0 22-23 38.72068779538505 40.0 38.0 41.0 34.0 41.0 24-25 38.65720920211287 40.0 38.0 41.0 34.0 41.0 26-27 38.496125104253544 40.0 38.0 41.0 34.0 41.0 28-29 38.311962468723934 40.0 38.0 41.0 34.0 41.0 30-31 38.12505921601334 40.0 37.5 41.0 33.0 41.0 32-33 37.95759855435085 40.0 37.0 41.0 33.0 41.0 34-35 37.921223102585486 40.0 37.0 41.0 33.0 41.0 36-37 37.80692910758965 40.0 37.0 41.0 33.0 41.0 38-39 37.67664553794829 40.0 37.0 41.0 33.0 41.0 40-41 37.50560592160133 40.0 37.0 41.0 32.5 41.0 42-43 37.324975118154015 40.0 37.0 41.0 32.0 41.0 44-45 37.057465387823186 40.0 36.0 41.0 31.0 41.0 46-47 36.718514595496245 40.0 36.0 41.0 30.5 41.0 48-49 36.407241729218796 39.5 36.0 41.0 30.0 41.0 50-51 36.131861551292744 39.0 35.0 41.0 29.5 41.0 52-53 35.89428718376425 39.0 35.0 40.5 28.5 41.0 54-55 35.640613288851824 39.0 35.0 40.0 28.0 41.0 56-57 35.377420628301365 38.0 35.0 40.0 28.0 41.0 58-59 35.068409785932715 38.0 34.0 40.0 27.0 41.0 60-61 34.66812649430081 38.0 34.0 40.0 26.0 41.0 62-63 34.35361996107868 37.0 34.0 40.0 26.0 41.0 64-65 34.09301529051988 37.0 34.0 40.0 26.0 41.0 66-67 33.648447317208785 36.0 33.0 39.0 25.0 41.0 68-69 33.23847080900751 36.0 33.0 39.0 24.0 40.0 70-71 32.787859049207675 35.0 32.0 38.5 23.0 40.0 72-73 32.278873505699195 35.0 32.0 37.5 22.0 39.5 74-75 31.811205448985266 35.0 31.0 37.0 20.5 39.0 76-77 29.822126077286626 33.0 28.5 35.0 18.0 37.5 78-79 30.812161662496525 34.0 30.5 36.0 18.0 38.0 80-81 30.820635946622186 34.0 31.0 36.0 18.0 37.0 82-83 30.49797094801223 34.0 31.0 35.0 17.5 37.0 84-85 30.104393939393937 34.0 30.0 35.0 10.5 36.5 86-87 29.5295355852099 34.0 29.5 35.0 6.0 36.0 88-89 28.966852793994995 34.0 29.0 35.0 2.0 35.5 90-91 28.511931192660548 34.0 29.0 35.0 2.0 35.0 92-93 28.154917987211565 33.0 29.0 35.0 2.0 35.0 94-95 27.77129802613289 33.0 27.5 35.0 2.0 35.0 96-97 27.15676257992772 33.0 26.5 35.0 2.0 35.0 98-99 26.269512927439536 33.0 25.0 34.5 2.0 35.0 100-101 21.94588240200167 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1842101223451138 1101 2 0.17472381215674204 1101 3 0.16583252856249686 1101 4 0.12178066562749024 1101 5 0.10041021148963125 1101 6 0.12310372186132668 1101 7 0.1327863261378397 1101 8 0.20757276994767437 1101 9 0.19131953832856397 1101 10-11 0.2466652397510245 1101 12-13 0.20255137903348697 1101 14-15 0.32537157472965106 1101 16-17 0.26437542152398663 1101 18-19 0.26579823983980333 1101 20-21 0.25671062948084256 1101 22-23 0.27542384082290283 1101 24-25 0.29183253747361704 1101 26-27 0.250840171010978 1101 28-29 0.30415049728432564 1101 30-31 0.14539051164805272 1101 32-33 0.19546756744686178 1101 34-35 0.4347786285013058 1101 36-37 0.31559890169087623 1101 38-39 0.2951071288591649 1101 40-41 0.31263376490625916 1101 42-43 0.3624143456246429 1101 44-45 0.3927845210918264 1101 46-47 0.2925697467127577 1101 48-49 0.08052768424040124 1101 50-51 0.10385943353546168 1101 52-53 0.1439077098151742 1101 54-55 0.14077760799114714 1101 56-57 0.05720257229879877 1101 58-59 0.18919720741828883 1101 60-61 0.21079058649301885 1101 62-63 0.14296259326201977 1101 64-65 0.15186540182460107 1101 66-67 0.19435736368931344 1101 68-69 0.14304547652564992 1101 70-71 0.15808691618420312 1101 72-73 -0.04889723347866237 1101 74-75 0.12199322213666619 1101 76-77 0.06655619185183781 1101 78-79 0.19966430546219627 1101 80-81 0.2970251166017377 1101 82-83 0.27041104873255506 1101 84-85 0.18977783630847966 1101 86-87 0.3078817585306908 1101 88-89 0.47089449427272 1101 90-91 0.3770515586562162 1101 92-93 0.40690386648608623 1101 94-95 0.29286103445994627 1101 96-97 0.0974118224946956 1101 98-99 0.060436893218039245 1101 100-101 0.6302695997357439 1102 1 0.11501268717719171 1102 2 0.11667058395597252 1102 3 0.12735037032559404 1102 4 0.12093294720935432 1102 5 0.18279373246291897 1102 6 0.1552279919622208 1102 7 0.15353897622561163 1102 8 0.18938553256135293 1102 9 0.20295727999121738 1102 10-11 0.25276571567329853 1102 12-13 0.21866431912611972 1102 14-15 0.23881381471912277 1102 16-17 0.26108373864808954 1102 18-19 0.17880887889948838 1102 20-21 0.22868076192232678 1102 22-23 0.24531290196507882 1102 24-25 0.2581725035261684 1102 26-27 0.09552722162828786 1102 28-29 0.17768416699542655 1102 30-31 0.08015006347984155 1102 32-33 0.16289718068985337 1102 34-35 0.1420027184136501 1102 36-37 0.17236255276378643 1102 38-39 0.2252095518242072 1102 40-41 0.2164274015989207 1102 42-43 0.16358228096579808 1102 44-45 0.18391386395521891 1102 46-47 0.2028340103493136 1102 48-49 -0.007647127684172972 1102 50-51 0.06022128930333537 1102 52-53 0.02791689536680053 1102 54-55 0.11652957100120176 1102 56-57 0.11787395367337439 1102 58-59 0.04790161110256719 1102 60-61 0.04128125675184435 1102 62-63 0.14703328678471195 1102 64-65 0.24573376547645154 1102 66-67 0.22691371857752785 1102 68-69 0.15728250091787288 1102 70-71 0.09024718528641529 1102 72-73 0.22304667388390698 1102 74-75 0.21412502106095488 1102 76-77 0.08611081065918924 1102 78-79 0.06069412850581557 1102 80-81 0.2478235783218281 1102 82-83 0.26379719995545514 1102 84-85 0.23298679889854768 1102 86-87 0.2554504711406018 1102 88-89 0.38111230758499914 1102 90-91 0.4295351026611236 1102 92-93 0.5711952764663266 1102 94-95 0.5794222782447918 1102 96-97 0.4448007336410349 1102 98-99 0.3615288629246862 1102 100-101 0.6989459203281392 1103 1 0.055372583724910385 1103 2 0.05493715880465544 1103 3 0.04930349057398331 1103 4 0.006937711691790582 1103 5 -0.002835931190510621 1103 6 0.01338238968605765 1103 7 -0.014568976600855876 1103 8 0.028826795179568876 1103 9 0.03363716662023819 1103 10-11 0.045116653807127705 1103 12-13 -0.004093541762344444 1103 14-15 0.04986452651530726 1103 16-17 -0.027555234374027293 1103 18-19 0.1247902122691471 1103 20-21 0.13986134868229527 1103 22-23 0.11770660712886638 1103 24-25 0.09974647807227655 1103 26-27 0.09913103713590488 1103 28-29 0.08653044888318107 1103 30-31 0.12776009604964855 1103 32-33 0.14712195799406658 1103 34-35 -0.012471183000243968 1103 36-37 -0.06685135708863754 1103 38-39 -0.051856979747277876 1103 40-41 -0.040755089947580814 1103 42-43 -0.008225360061857145 1103 44-45 -0.0021424114938852767 1103 46-47 0.16356390434881973 1103 48-49 0.07946176053009424 1103 50-51 -0.04007425888067928 1103 52-53 0.010448847219450386 1103 54-55 0.07691419315997905 1103 56-57 6.993887597062098E-4 1103 58-59 -0.09646978114506766 1103 60-61 -0.05127827606963109 1103 62-63 0.04038944794141486 1103 64-65 0.0048258154957991906 1103 66-67 0.09991398120798323 1103 68-69 0.028661604019710296 1103 70-71 -0.010318758598472755 1103 72-73 0.13164681022268354 1103 74-75 0.11413735002905767 1103 76-77 0.022438501269935074 1103 78-79 0.13467995374976383 1103 80-81 0.09659577013905363 1103 82-83 0.1778973054501236 1103 84-85 0.1253739083680685 1103 86-87 0.11029921309564372 1103 88-89 0.18545809097813049 1103 90-91 0.32919005640320265 1103 92-93 0.411130175003084 1103 94-95 0.339306818022461 1103 96-97 0.11648916283072097 1103 98-99 0.37013554116490965 1103 100-101 1.0165130253571526 1104 1 0.024851446144850797 1104 2 0.045975468861165325 1104 3 0.08342066277254645 1104 4 0.025054310053192808 1104 5 0.0818639271390964 1104 6 0.03089779636275125 1104 7 0.009588977287570799 1104 8 0.06114968417872291 1104 9 0.1393554608580203 1104 10-11 0.06168950168765264 1104 12-13 0.15982645554214514 1104 14-15 0.16184987282620966 1104 16-17 0.15286957185606553 1104 18-19 0.11803446942531082 1104 20-21 0.09726258017435185 1104 22-23 0.036943021612273697 1104 24-25 0.06457706845588262 1104 26-27 0.08659124498514359 1104 28-29 0.06907439718504094 1104 30-31 0.07502526005262666 1104 32-33 0.08596922265439844 1104 34-35 0.07791507332190406 1104 36-37 0.053967894453336385 1104 38-39 0.16024284742422168 1104 40-41 0.22154654415098918 1104 42-43 0.1982809442444804 1104 44-45 0.22417295475021604 1104 46-47 0.15848825041500447 1104 48-49 0.05794508147717892 1104 50-51 -0.11030309555262363 1104 52-53 -0.15692507143010204 1104 54-55 -0.05393557213819378 1104 56-57 -0.030295459731824792 1104 58-59 0.05988638589364825 1104 60-61 0.04371174690991353 1104 62-63 0.05725299193122879 1104 64-65 0.0959416995132969 1104 66-67 0.15361916472558335 1104 68-69 0.11615878824037651 1104 70-71 -0.20860107675470374 1104 72-73 -0.10400213725332463 1104 74-75 -0.01880182340575587 1104 76-77 -0.1396520427308161 1104 78-79 0.0568901959441277 1104 80-81 0.1880439030928187 1104 82-83 0.21456474441176 1104 84-85 0.19122832219879626 1104 86-87 0.07108476386208551 1104 88-89 0.16835217720758067 1104 90-91 0.3087456747535562 1104 92-93 0.26912558152693933 1104 94-95 0.2959929674795987 1104 96-97 0.24828639337171055 1104 98-99 0.3585804685156333 1104 100-101 0.9934441289463969 1105 1 0.017311856237014922 1105 2 0.026659969843557008 1105 3 0.042812976657955915 1105 4 -0.005653942491932185 1105 5 0.13614968906404812 1105 6 0.07939930414461571 1105 7 0.003667249916041726 1105 8 0.01893485435628861 1105 9 0.0026512658148121204 1105 10-11 -0.02302335621062923 1105 12-13 0.009663920671926007 1105 14-15 0.05548702312868414 1105 16-17 0.0868837833211984 1105 18-19 0.039711649618006106 1105 20-21 0.08075013986802304 1105 22-23 0.0016061917857612684 1105 24-25 0.05676959290671846 1105 26-27 0.019182829717991012 1105 28-29 -0.02145648767296393 1105 30-31 0.033570105929491945 1105 32-33 -0.03726761513468091 1105 34-35 0.04826883549458216 1105 36-37 0.024027569404012183 1105 38-39 0.02947186421160808 1105 40-41 0.08725570184163445 1105 42-43 0.07959433420498385 1105 44-45 0.030425876907024474 1105 46-47 0.1288881205640564 1105 48-49 0.12591887241034527 1105 50-51 0.05341612014219521 1105 52-53 0.15480908529074355 1105 54-55 -0.035835043819041346 1105 56-57 0.04877954960887365 1105 58-59 -0.023588957738382987 1105 60-61 0.054058649032370454 1105 62-63 0.051769460475377116 1105 64-65 0.07558758004633859 1105 66-67 0.20167005636196933 1105 68-69 0.1324451973783951 1105 70-71 0.11943794930872542 1105 72-73 0.020024065688467374 1105 74-75 0.1006810424618898 1105 76-77 -0.16819900515094233 1105 78-79 0.09870485617087965 1105 80-81 0.056572027597283636 1105 82-83 0.2063251979917453 1105 84-85 0.05848065090681587 1105 86-87 0.08685488276281461 1105 88-89 0.16285137652810988 1105 90-91 0.2857499595626969 1105 92-93 0.4481659163082803 1105 94-95 0.2980964950290286 1105 96-97 0.3023126065219053 1105 98-99 0.4896309919687667 1105 100-101 1.003274825901638 1106 1 0.004796348551316498 1106 2 -0.05805544037706056 1106 3 -0.019465393527639208 1106 4 0.006253151809666235 1106 5 0.09182413917100973 1106 6 0.03935536226023828 1106 7 0.012469916164214112 1106 8 0.017916922433173 1106 9 -0.03578912767980569 1106 10-11 0.056672891383726665 1106 12-13 0.04435806202933179 1106 14-15 0.0672340847216617 1106 16-17 -0.013666316208819751 1106 18-19 -0.12098200441399598 1106 20-21 -0.06674517272231384 1106 22-23 0.08165992147709744 1106 24-25 0.06399675262555604 1106 26-27 0.04921533184487714 1106 28-29 0.029870028611135524 1106 30-31 0.09794637070411483 1106 32-33 0.14691475194525339 1106 34-35 0.1653799869867072 1106 36-37 0.049142462564105927 1106 38-39 0.1300892394273987 1106 40-41 0.18830704446443036 1106 42-43 0.14591490346714409 1106 44-45 0.1946333949339234 1106 46-47 0.2789504816969597 1106 48-49 0.11617108395913078 1106 50-51 0.16187394171501523 1106 52-53 0.2344268239356424 1106 54-55 0.20910899635639169 1106 56-57 0.19355864252283084 1106 58-59 0.2571783732877293 1106 60-61 0.16318567576919207 1106 62-63 0.207708016175026 1106 64-65 0.1395511462771708 1106 66-67 0.34465864138373803 1106 68-69 0.3702933253054468 1106 70-71 0.27089711722123866 1106 72-73 0.3490268657446265 1106 74-75 0.4439309660591739 1106 76-77 0.210949083955267 1106 78-79 0.32944980326716333 1106 80-81 0.47561918252546675 1106 82-83 0.5226278848892996 1106 84-85 0.3958188217019796 1106 86-87 0.5095406239517253 1106 88-89 0.5760823439929439 1106 90-91 0.807210490334402 1106 92-93 0.8081208180585193 1106 94-95 0.8988437135573051 1106 96-97 0.8311182049822925 1106 98-99 1.0029574008760846 1106 100-101 1.1916632655517496 1107 1 -0.06910770367538532 1107 2 -0.06512279501124141 1107 3 -0.0833508575584645 1107 4 -0.06005269959098314 1107 5 -0.1367322320863238 1107 6 -0.034620428146617144 1107 7 -0.17159276895174713 1107 8 -0.07298205174353711 1107 9 -0.04132456985417576 1107 10-11 -0.015432640545078868 1107 12-13 -0.06774983938377233 1107 14-15 -0.004365317187819073 1107 16-17 -0.006934733842683727 1107 18-19 -0.009278917139347698 1107 20-21 -0.09530967376203137 1107 22-23 -0.02514928192678667 1107 24-25 -0.0963898001424468 1107 26-27 -0.13783944001055204 1107 28-29 -0.10466888199802327 1107 30-31 -0.09510948784583917 1107 32-33 -0.009451398745781603 1107 34-35 -0.13851037942544053 1107 36-37 -0.08637124571968968 1107 38-39 -0.029602033108240278 1107 40-41 -0.1715091979419796 1107 42-43 -0.1902060055977728 1107 44-45 -0.311207751569647 1107 46-47 -0.43517695570575654 1107 48-49 -0.3125095626649639 1107 50-51 -0.2972323541682158 1107 52-53 -0.2973021642537006 1107 54-55 -0.4909697685703378 1107 56-57 -0.4142647818722409 1107 58-59 -0.3687945247141471 1107 60-61 -0.5136182984279358 1107 62-63 -0.5020095249539125 1107 64-65 -0.498656502254768 1107 66-67 -0.3170107011660761 1107 68-69 -0.49528668768586925 1107 70-71 -0.52739097121772 1107 72-73 -0.47096337034588487 1107 74-75 -0.5289376215239123 1107 76-77 -0.6859447672329466 1107 78-79 -0.5020071844646274 1107 80-81 -0.41392064728019307 1107 82-83 -0.40244411883650955 1107 84-85 -0.4623567979385932 1107 86-87 -0.44114852830398377 1107 88-89 -0.318902969289649 1107 90-91 -0.13162221181733358 1107 92-93 -0.046062261372313174 1107 94-95 -0.07062298906015485 1107 96-97 -0.046188222448169114 1107 98-99 -0.20908035563080318 1107 100-101 0.3783081574746703 1108 1 0.012735061004462978 1108 2 -0.00709385343462543 1108 3 -0.011694775964350868 1108 4 0.031212284416696434 1108 5 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-0.9357214361544735 2316 50-51 -1.0271081422423904 2316 52-53 -1.0094651068882996 2316 54-55 -1.0741733470521595 2316 56-57 -1.211232937182686 2316 58-59 -1.0473573564902239 2316 60-61 -0.8793544900503676 2316 62-63 -0.9868877065641968 2316 64-65 -1.0082592334054326 2316 66-67 -1.1736816897643152 2316 68-69 -1.155388572862023 2316 70-71 -1.1394313299186614 2316 72-73 -1.1179455767791673 2316 74-75 -1.3341278155844556 2316 76-77 -1.3510983702315542 2316 78-79 -1.3525035282655615 2316 80-81 -1.3150764248111884 2316 82-83 -1.4734958986777045 2316 84-85 -1.624148112600409 2316 86-87 -1.6452097873233527 2316 88-89 -1.8331821079267456 2316 90-91 -2.0065806883267605 2316 92-93 -2.4670718878450195 2316 94-95 -2.618171698004229 2316 96-97 -2.9295570227536096 2316 98-99 -3.2586876994259235 2316 100-101 -3.8758300435552293 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 17.0 4 32.0 5 79.0 6 221.0 7 536.0 8 1188.0 9 2087.0 10 3695.0 11 5659.0 12 8170.0 13 11957.0 14 14081.0 15 18385.0 16 23941.0 17 34190.0 18 33097.0 19 21773.0 20 19121.0 21 20225.0 22 21909.0 23 24184.0 24 27792.0 25 33112.0 26 39457.0 27 48267.0 28 58848.0 29 71789.0 30 87288.0 31 106346.0 32 132539.0 33 167719.0 34 225609.0 35 322869.0 36 507332.0 37 817902.0 38 637985.0 39 47594.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.807901602350626 20.05177031243632 14.22899193325521 22.911336151957844 2 15.726105087572979 21.4868501529052 39.23742007228246 23.549624687239366 3 18.753655824298026 25.629051987767582 30.88885182096191 24.728440366972475 4 12.84890186266333 17.18868501529052 37.1147067000278 32.84770642201835 5 12.998038081295215 38.268312747133784 34.60905774246182 14.124591429109179 6 31.09963858771198 36.764442591048095 17.478704475952185 14.65721434528774 7 27.502085070892413 33.825132054489856 21.33705865999444 17.335724214623298 8 25.94359188212399 35.49894356408118 20.14756741729219 18.40989713650264 9 26.484820683903255 16.349735891020295 20.950291909924935 36.21515151515152 10-11 24.57631359466222 26.98550180706144 27.577175423964416 20.861009174311924 12-13 25.512663330553238 24.656005004170144 28.217778704475954 21.613552960800668 14-15 22.4730171637875 25.55183714036496 26.868670440684326 25.106475255163215 16-17 21.44027194445456 28.988137321162522 27.600251994002633 21.971338740380286 18-19 21.357061440088962 27.357339449541286 30.172449263274952 21.1131498470948 20-21 22.112149013066446 26.647192104531552 30.04228523769808 21.19837364470392 22-23 21.94118710036141 26.679441201000838 29.87121212121212 21.50815957742563 24-25 21.758673894912427 26.756602724492634 30.06488740617181 21.41983597442313 26-27 21.636196830692246 26.906491520711707 30.005018070614405 21.452293577981653 28-29 21.378329163191548 26.863775368362525 30.31516541562413 21.442730052821794 30-31 21.46120378092855 26.94348067834306 30.14246594384209 21.452849596886296 32-33 21.346594384209062 27.083569641367806 29.918306922435363 21.651529051987765 34-35 21.772217285132697 26.889757316217594 29.87225054967113 21.465774848978576 36-37 21.889043905118946 26.684782064727212 29.746805176664747 21.679368853489088 38-39 21.65440288675324 26.965357098221315 29.95505574552841 21.425184269497034 40-41 21.660401110507507 26.83846486182322 30.023764816172978 21.4773692114963 42-43 21.557215096335007 27.05873456586764 29.630573322780034 21.753477015017324 44-45 21.664875582554107 27.2280926504111 29.358891971564503 21.748139795470287 46-47 21.733977575695235 27.281643818859656 28.97287732242792 22.01150128301719 48-49 21.922299990992464 27.38436992283544 28.754446776024828 21.93888331014727 50-51 21.84745865905806 27.632174110552217 28.55714475953293 21.963222470856795 52-53 21.932554906866834 27.68190158465388 28.497942730052824 21.887600778426467 54-55 21.564512093411174 27.590436474840146 28.584417570197385 22.260633861551295 56-57 21.694036697247707 28.011537392271336 28.30048651654156 21.993939393939392 58-59 21.797256264536443 27.4853669480683 28.530420123024946 22.186956664370307 60-61 21.886348677237276 27.46287972565436 28.59466669076134 22.056104906347024 62-63 21.80561579093689 27.43122046149569 28.7210175145955 22.04214623297192 64-65 21.65878855738509 27.376078765802266 28.728694874954563 22.23643780185808 66-67 21.43899496822439 27.702330952884687 28.41805022656752 22.440623852323405 68-69 21.383592720479076 27.570240259776362 28.578889278108615 22.467277741635947 70-71 21.362996941896025 27.59306366416458 28.48052543786489 22.563413956074506 72-73 21.495246038365305 27.48614122880178 28.279413400055603 22.739199332777314 74-75 21.36099527383931 27.6899638587712 28.11121768140117 22.837823185988324 76-77 21.391130143449573 27.413401097068824 28.415243277488933 22.780225481992673 78-79 21.24140741807474 27.42448008033366 28.330596792102746 23.003515709488855 80-81 21.07146232971921 27.726285793716986 28.29667778704476 22.905574089519043 82-83 21.181804281345563 27.538851820961913 28.217125382263 23.062218515429525 84-85 21.057019738671116 27.352474284125663 28.083833750347516 23.506672226855713 86-87 21.04486440336548 27.162370581845565 28.398874015247095 23.39389099954186 88-89 21.172626248594252 26.968937760338314 28.30903218195183 23.549403809115603 90-91 20.889290143116746 27.069276282086065 28.481461185278516 23.559972389518673 92-93 20.791951626355296 27.14339727550737 28.31330275229358 23.75134834584376 94-95 20.69314706700028 26.852919099249373 28.353183208229083 24.10075062552127 96-97 20.724181200012843 26.679583034298293 28.254234482114406 24.342001283574458 98-99 20.522393661384484 26.633986655546288 28.2527661940506 24.590853489018627 100-101 20.497872108553043 26.260665868220855 28.874380453868625 24.367081569357474 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1922.0 1 1392.0 2 1060.5 3 1280.0 4 1133.0 5 1129.5 6 1915.5 7 3649.5 8 5546.0 9 6465.5 10 5896.5 11 5164.0 12 5260.5 13 5635.0 14 6050.5 15 6382.5 16 6678.5 17 6959.5 18 7340.0 19 7811.5 20 8674.0 21 10057.0 22 11874.5 23 14122.5 24 16471.0 25 19286.0 26 23675.5 27 28959.5 28 32727.0 29 37170.5 30 43510.0 31 49901.5 32 56133.0 33 63641.5 34 73855.5 35 81063.0 36 88812.5 37 99008.5 38 102944.0 39 103491.0 40 105032.5 41 108964.5 42 113056.0 43 117443.5 44 123687.0 45 128394.5 46 129492.0 47 131918.0 48 146924.0 49 149829.5 50 138323.0 51 133002.5 52 126235.0 53 121664.0 54 115982.0 55 105394.5 56 94628.0 57 83964.0 58 74477.0 59 65865.5 60 56181.0 61 44895.5 62 35387.5 63 27746.0 64 20454.0 65 14885.0 66 10181.5 67 7184.0 68 4800.5 69 2746.5 70 1793.0 71 1154.5 72 768.0 73 505.0 74 360.5 75 262.0 76 159.5 77 72.5 78 34.0 79 19.5 80 13.5 81 10.0 82 4.0 83 4.0 84 4.5 85 3.0 86 1.0 87 1.0 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.40695023630803445 2 0.0 3 0.0 4 0.0 5 3.058103975535168E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001042535446205171 16-17 0.0024742841256602723 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0021267723102585488 36-37 0.0067834306366416465 38-39 0.012051709758131777 40-41 0.002390881289963859 42-43 5.838198498748957E-4 44-45 4.587155963302752E-4 46-47 2.7800945232137893E-4 48-49 3.336113427856547E-4 50-51 5.838198498748957E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 1.6680567139282736E-4 60-61 0.0021545732554906867 62-63 0.0 64-65 2.085070892410342E-4 66-67 0.0016402557686961356 68-69 0.0016680567139282735 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.125604670558799 78-79 5.560189046427579E-5 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.14733110925771478 88-89 0.43710036141228803 90-91 0.33721156519321654 92-93 0.0 94-95 0.0 96-97 3.7531276063386155E-4 98-99 0.0 100-101 3.336113427856547E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3597000.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.76244159960262 #Duplication Level Percentage of deduplicated Percentage of total 1 65.65194958955367 26.104818114546823 2 15.672854765694602 12.463819446399704 3 6.6861468033967055 7.975725653892936 4 3.5270675390560884 5.609792681582873 5 2.1333279775523484 4.241316456011181 6 1.3470916309039251 3.2138191381878456 7 0.9372629567159316 2.6087504505921677 8 0.7002773884579465 2.227579100966507 9 0.5385099591405826 1.9271223721018627 >10 2.528757945250927 17.901608016419477 >50 0.16890905990158056 4.652105486532173 >100 0.1010543649504415 7.405689906447309 >500 0.0050400144215885885 1.3854825025661133 >1k 0.0015400044033031996 1.1123773655676104 >5k 7.000020015014545E-5 0.27625035289024225 >10k+ 1.400004003002909E-4 0.8937429552952826 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17134 0.47634139560745065 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14976 0.41634695579649705 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9925 0.2759243814289686 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4083 0.11351125938281902 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.7800945232137893E-5 0.0 2 0.0 0.0 0.0 2.7800945232137893E-5 0.0 3 0.0 0.0 0.0 2.7800945232137893E-5 0.0 4 0.0 0.0 0.0 5.560189046427579E-5 0.0 5 0.0 0.0 0.0 5.560189046427579E-5 0.0 6 0.0 0.0 0.0 5.560189046427579E-5 0.0 7 0.0 0.0 0.0 8.340283569641368E-5 0.0 8 0.0 0.0 0.0 8.340283569641368E-5 0.0 9 0.0 0.0 0.0 1.1120378092855157E-4 0.0 10-11 0.0 0.0 0.0 1.1120378092855157E-4 0.0 12-13 0.0 0.0 0.0 1.2510425354462053E-4 0.0 14-15 0.0 0.0 0.0 1.6680567139282736E-4 0.0 16-17 0.0 0.0 0.0 2.3630803447317208E-4 0.0 18-19 0.0 0.0 0.0 2.50208507089241E-4 0.0 20-21 0.0 0.0 0.0 2.6410897970531E-4 0.0 22-23 0.0 0.0 0.0 6.255212677231026E-4 0.0 24-25 0.0 0.0 0.0 0.0011120378092855157 0.0 26-27 0.0 0.0 0.0 0.0019738671114817906 0.0 28-29 0.0 0.0 0.0 0.00619961078676675 0.0 30-31 0.0 0.0 0.0 0.017500695023630802 0.0 32-33 0.0 0.0 0.0 0.03894912427022519 0.0 34-35 0.0 0.0 0.0 0.07218515429524604 0.0 36-37 0.0 0.0 0.0 0.12955240478176258 0.0 38-39 0.0 0.0 0.0 0.2130247428412566 0.0 40-41 0.0 0.0 0.0 0.3052821795941062 0.0 42-43 0.0 0.0 0.0 0.3992215735335002 0.0 44-45 0.0 0.0 0.0 0.5151098137336669 0.0 46-47 0.0 0.0 0.0 0.6570614400889631 0.0 48-49 0.0 0.0 0.0 0.811884904086739 0.0 50-51 0.0 0.0 0.0 0.9787878787878788 0.0 52-53 0.0 0.0 0.0 1.143522379760912 0.0 54-55 0.0 0.0 0.0 1.3282457603558522 0.0 56-57 0.0 0.0 0.0 1.5465387823185988 0.0 58-59 0.0 0.0 0.0 1.7948151237142063 0.0 60-61 0.0 0.0 0.0 2.073547400611621 0.0 62-63 0.0 0.0 0.0 2.3572282457603557 0.0 64-65 0.0 0.0 0.0 2.6515012510425358 0.0 66-67 0.0 0.0 0.0 2.985098693355574 2.7800945232137893E-5 68-69 0.0 0.0 0.0 3.3627884348067836 2.7800945232137893E-5 70-71 0.0 0.0 0.0 3.779677509035307 2.7800945232137893E-5 72-73 0.0 0.0 0.0 4.205309980539338 2.7800945232137893E-5 74-75 0.0 0.0 0.0 4.640770086182931 2.7800945232137893E-5 76-77 0.0 0.0 0.0 5.113274951348346 2.7800945232137893E-5 78-79 0.0 0.0 0.0 5.6446483180428135 2.7800945232137893E-5 80-81 0.0 0.0 0.0 6.232276897414512 2.7800945232137893E-5 82-83 0.0 0.0 0.0 6.8305810397553515 2.7800945232137893E-5 84-85 0.0 0.0 0.0 7.449902696691687 2.7800945232137893E-5 86-87 0.0 0.0 0.0 8.104434250764527 2.7800945232137893E-5 88-89 0.0 0.0 0.0 8.803767028078955 2.7800945232137893E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15550 0.0 31.687387 1 GTATCAA 22100 0.0 28.204067 1 GTACATG 16080 0.0 25.48641 1 TACATGG 16625 0.0 23.973219 2 ATCAACG 26900 0.0 23.05324 3 TATCAAC 27080 0.0 22.98761 2 TCAACGC 27040 0.0 22.986523 4 ACATGGG 16585 0.0 22.943916 3 CAACGCA 27460 0.0 22.634945 5 AACGCAG 27915 0.0 22.24901 6 GAGTACT 14840 0.0 21.679073 12-13 CATGGGG 6265 0.0 20.220825 4 AGTACTT 15315 0.0 19.937765 12-13 GTACTTT 16185 0.0 19.936142 14-15 ACGCAGA 30825 0.0 19.840769 7 CGCAGAG 30680 0.0 19.578842 8 GCAGAGT 31720 0.0 18.578178 9 GTATAGG 935 0.0 18.347933 1 CATGGGA 10785 0.0 18.21331 4 AGTACAT 12245 0.0 18.09534 2 >>END_MODULE