FastQCFastQC Report
Fri 27 May 2016
SRR939047_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939047_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3189526
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90320.28317687330343133No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73600.2307552909115649No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT71540.22429665097572493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44950.14093003160971254No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT36500.11443706682434944No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG95550.022.7547262
GTACATG101050.022.2626861
ACATGGG96500.021.647163
GAGTACT86450.020.45745812-13
GTATCAA185350.020.3056141
CATGGGG69350.019.7397214
AGAGTAC130350.019.37950310-11
GGTATCA146950.019.1845861
GTACTTT92850.018.81861114-15
AGTACTT91700.018.07277312-13
CAACGCA207550.017.8701695
ATCAACG210100.017.743463
TCAACGC210050.017.702584
TATCAAC211150.017.6552262
AACGCAG212200.017.4943086
GTATAGG7400.017.31681
GAGTACA82500.015.992871
ACGCAGA230000.015.9757027
GTGTAGC16350.015.96542551
CGCAGAG231200.015.8723048
Produced by FastQC (version 0.11.5)